Rh2AG476600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0017466
Physical Location & Seq
Reverse (-)
69575779 .. 69581753
5975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG476600.1

Sequence Viewer

Length: 546 bp
ATGAGGGGGACACTTGCAGAAAAGGATAGGTTGCTAGCGGAACAAAATCAGACGATAGAAAAAATGAAAGAAGATTTGAAAGAACTCTCTGACAGTGGAGAGGATTCTCTATGTCATTGGGCTTTGTTCCTGGTGAGGTTTGCTATGGAAATAACTCTCTCCGTTCTTGAACCTTTCACTTTAGGTAAGCATAATCTACGCTTTTTAATCCCATCAATGGTGATTTTTGTGGTGGTTCTTGGGATGAACTGTGTTAAGCTTTACAGATATCATAAGAAAAAACCATGGTTTGAAAATGGCAAGCTAAAGATGAGAAGGGTCTTGTGGATGCCAGAGACATACGGGTTCCTCTCCACGGTTGCTGAAGCTCTGACGGCGGCTGCCGCCTATGGAGAAGAGGTCGCCAACGAACACATTAGGGAGGGCGTGGCTGCTGTGGTTGTGGAGACTTTGTTCCCAGCTCACTCCGACCCGTACAGTATGATCTCAACCCGAGTAACTTCGGCTGCATTTGGAATTGATCGGTCTATGTTTTCATTGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.54

Weight (kDa)

6.52

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 38, 377, 384
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 2 cut(s) 217, 355
AgsI TTSAA 3 cut(s) 79, 170, 293
AjnI CCWGG 1 cut(s) 129
AloI GAACNNNNNNTCC 2 cut(s) 437, 469
AluBI AGCT 4 cut(s) 259, 304, 368, 461
AluI AGCT 4 cut(s) 259, 304, 368, 461
Alw26I GTCTC 2 cut(s) 329, 440
Ama87I CYCGRG 1 cut(s) 492
ApeKI GCWGC 3 cut(s) 380, 431, 506
AsuHPI GGTGA 2 cut(s) 145, 232
AsuNHI GCTAGC 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 492
BbvI GCAGC 3 cut(s) 367, 418, 493
BccI CCATC 1 cut(s) 220
BceAI ACGGC 1 cut(s) 390
BciT130I CCWGG 1 cut(s) 131
BcoDI GTCTC 2 cut(s) 329, 440
BfaI CTAG 1 cut(s) 35
BisI GCNGC 5 cut(s) 378, 381, 384, 432, 507
BlsI GCNGC 5 cut(s) 379, 382, 385, 433, 508
Bme1390I CCNGG 1 cut(s) 131
BmeT110I CYCGRG 1 cut(s) 492
BmiI GGNNCC 1 cut(s) 347
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 1 cut(s) 318
BmtI GCTAGC 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 284, 354
BsaXI ACNNNNNCTCC 4 cut(s) 384, 414, 437, 467
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 355
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 2 cut(s) 284, 354
BseGI GGATG 2 cut(s) 249, 333
BseLI CCNNNNNNNGG 2 cut(s) 217, 355
BseXI GCAGC 3 cut(s) 367, 418, 493
BseYI CCCAGC 1 cut(s) 457
BsiHKCI CYCGRG 1 cut(s) 492
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 2 cut(s) 217, 355
BsmAI GTCTC 2 cut(s) 329, 440
BsmFI GGGAC 1 cut(s) 22
BsoBI CYCGRG 1 cut(s) 492
Bsp143I GATC 2 cut(s) 483, 520
Bsp19I CCATGG 1 cut(s) 284
BspACI CCGC 3 cut(s) 38, 377, 384
BspLI GGNNCC 1 cut(s) 347
BspOI GCTAGC 1 cut(s) 38
BssECI CCNNGG 2 cut(s) 284, 354
BssMI GATC 2 cut(s) 483, 520
BssT1I CCWWGG 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 4 cut(s) 95, 251, 358, 479
Bst6I CTCTTC 1 cut(s) 390
BstC8I GCNNGC 2 cut(s) 36, 302
BstDSI CCRYGG 2 cut(s) 284, 354
BstF5I GGATG 2 cut(s) 249, 333
BstKTI GATC 2 cut(s) 486, 523
BstMAI GTCTC 2 cut(s) 329, 440
BstMBI GATC 2 cut(s) 483, 520
BstMWI GCNNNNNNNGC 2 cut(s) 374, 383
BstNI CCWGG 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 129
BstV1I GCAGC 3 cut(s) 367, 418, 493
BtgI CCRYGG 2 cut(s) 284, 354
BtsCI GGATG 2 cut(s) 249, 333
BtsIMutI CAGTG 1 cut(s) 100
Cac8I GCNNGC 2 cut(s) 36, 302
Csp6I GTAC 1 cut(s) 475
CviAII CATG 1 cut(s) 285
CviJI RGCY 8 cut(s) 122, 259, 304, 368, 380, 431, 461, 506
CviKI_1 RGCY 8 cut(s) 122, 259, 304, 368, 380, 431, 461, 506
CviQI GTAC 1 cut(s) 475
DpnI GATC 2 cut(s) 485, 522
DpnII GATC 2 cut(s) 483, 520
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 284
Eco32I GATATC 1 cut(s) 269
Eco57I CTGAAG 1 cut(s) 384
Eco88I CYCGRG 1 cut(s) 492
EcoRII CCWGG 1 cut(s) 129
EcoRV GATATC 1 cut(s) 269
EcoT14I CCWWGG 1 cut(s) 284
ErhI CCWWGG 1 cut(s) 284
FaeI CATG 1 cut(s) 288
FaiI YATR 9 cut(s) 112, 146, 192, 273, 286, 340, 390, 482, 530
FaqI GGGAC 1 cut(s) 22
FatI CATG 1 cut(s) 284
Fnu4HI GCNGC 5 cut(s) 378, 381, 384, 432, 507
FokI GGATG 2 cut(s) 256, 340
Fsp4HI GCNGC 5 cut(s) 378, 381, 384, 432, 507
FspBI CTAG 1 cut(s) 35
GluI GCNGC 5 cut(s) 378, 381, 384, 432, 507
GsaI CCCAGC 1 cut(s) 461
Hin1II CATG 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 1 cut(s) 104
HphI GGTGA 2 cut(s) 145, 232
Hpy188I TCNGA 4 cut(s) 51, 91, 372, 469
Hpy188III TCNNGA 1 cut(s) 167
HpyAV CCTTC 1 cut(s) 309
HpyCH4III ACNGT 4 cut(s) 95, 251, 358, 479
HpyCH4V TGCA 2 cut(s) 17, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 374, 383
Hsp92II CATG 1 cut(s) 288
Kzo9I GATC 2 cut(s) 483, 520
LpnPI CCDG 4 cut(s) 116, 143, 345, 471
Lsp1109I GCAGC 3 cut(s) 367, 418, 493
LweI GCATC 1 cut(s) 318
MaeI CTAG 1 cut(s) 35
MaeIII GTNAC 1 cut(s) 496
MalI GATC 2 cut(s) 485, 522
MboI GATC 2 cut(s) 483, 520
MboII GAAGA 2 cut(s) 83, 407
MluCI AATT 1 cut(s) 516
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 5 cut(s) 94, 129, 359, 391, 415
MseI TTAA 2 cut(s) 206, 255
MspR9I CCNGG 1 cut(s) 131
MvaI CCWGG 1 cut(s) 131
MwoI GCNNNNNNNGC 2 cut(s) 374, 383
NcoI CCATGG 1 cut(s) 284
NdeII GATC 2 cut(s) 483, 520
NheI GCTAGC 1 cut(s) 34
NlaIII CATG 1 cut(s) 288
NlaIV GGNNCC 1 cut(s) 347
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 5 cut(s) 379, 382, 385, 433, 508
Psp6I CCWGG 1 cut(s) 129
PspFI CCCAGC 1 cut(s) 457
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 1 cut(s) 347
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
SaqAI TTAA 2 cut(s) 206, 255
SatI GCNGC 5 cut(s) 378, 381, 384, 432, 507
Sau3AI GATC 2 cut(s) 483, 520
ScrFI CCNGG 1 cut(s) 131
SetI ASST 9 cut(s) 32, 140, 175, 187, 261, 306, 370, 402, 463
SfaNI GCATC 1 cut(s) 318
Sse9I AATT 1 cut(s) 516
SsiI CCGC 3 cut(s) 38, 377, 384
SspMI CTAG 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 129
StyI CCWWGG 1 cut(s) 284
TaaI ACNGT 4 cut(s) 95, 251, 358, 479
TaqII GACCGA 1 cut(s) 513
TasI AATT 1 cut(s) 516
TauI GCSGC 2 cut(s) 380, 386
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 2 cut(s) 206, 255
Tru9I TTAA 2 cut(s) 206, 255
TscAI CASTG 1 cut(s) 100
TseI GCWGC 3 cut(s) 380, 431, 506
TspDTI ATGAA 3 cut(s) 80, 260, 525
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 1 cut(s) 100
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.