Rmu_ssc0000091.1_g000016

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000091.1
OG0010272
Physical Location & Seq
Reverse (-)
84103 .. 88915
4813 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1296 bp
atgatgatccgcagtgccatcgccagaagactttcctcaaagccaacctcgtcttcatcttcttatgcttggttgaccaccaccactagtacgacgtcgtttgacttggttgatcaaaacctccatcagaagaggaggagcagcacgacctcattacctgttgggctgccttcttacatgagagcagccgtcttctgggaaccaaacaagcctctctccattgaagaattccacatccctcgccccaaatccggtgaaattctcatcaaaaccaaggcctgtggagtctgccattctgatctccatgttttgaagggtgagcttccattcgctagcccttgtgtcgtgggccacgagattactggtgaggtcgtcgaacatggacccgctacagacaaaaagatcacagaaagattaccgatcggatctaatgttgttggagcttttatcatgccttgtggtagctgtttcttctgttcaaagggccacgacgatttatgtgaggatttctttgcctacaaccgtgccaaaggaaccctttatgatggtgaaactcggttattcctccggagcagtggaaaacctgtatacatgtatagtatgggaggccttgctgagtactgtgttgtaccagcaaatggattggctattttaccgaagtcattgccatatacagagtctgccattttaggctgtgcagtatttactgcttatggtgctatggcacatgcggctgaggttcgtcctggggattcagttgctattattggaactgggggtgttggatccagctgtctgcagatagccagagcatttggtgcctccgacattattgcagtcgatattcaagacgacaaactgcagaaagctaagatatttggtgctactcatgttataaatgcggctaaagaagatgctgttgaaaaattattggaaataactggtgggaaaggtgtggacgttgctgtggaagccctcggaaaaccacagacctttttgcaatgcacgcaaagtgtgagagatggaggaaaagctgtcttcatcgggcttacagcagccggagcaattggagaagtagatattaaccgtcttgttcgtagaaagataaaaataattggatcatacggaggaagggcaaggcaggatcttccaaagatagttagactagcagaaagtggtatatttgatctcaccagtgctgtttcaagaaaatacaaattcgaggagtctgacactgcattccaggatctcaaccagggaaaaattgttggtcgggcagtggttgagataatgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

431

Amino Acids

46.2

Weight (kDa)

8.53

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 84 - 219 7.5e-23 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 242 - 418 3.7e-06 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 260 - 391 4.8e-33 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 293 - 428 3.2e-14 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63620 AT5G63620 AT5G63620
fragaria_vesca FvH4_1g25480 FvH4_1g25480 FvH4_1g25480 FvH4_1g25480
malus_domestica MD00G1129200.v1.1
prunus_persica Prupe.4G281800_v2.0.a1
pyrus_communis pycom520g00640
rosa_chinensis RchiOBHm_Chr2g0123611
rosa_laevigata RLG00000018760
rosa_multiflora Rmu_sc0005477.1_g000001 Rmu_ssc0000091.1_g000016
rosa_roxburghii Rroxscaffold_2G00120540
rosa_rugosa Rorug02G0246400
rosa_samantha Rh2AG305700 Rh2BG313600 Rh2CG291800 Rh2DG329500
rosa_wichuraiana Rw2G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 896
AatII GACGTC 1 cut(s) 98
AccB1I GGYRCC 1 cut(s) 818
AccB7I CCANNNNNTGG 1 cut(s) 638
AccI GTMKAC 1 cut(s) 588
AccIII TCCGGA 1 cut(s) 567
AciI CCGC 4 cut(s) 10, 387, 731, 902
AclWI GGATC 6 cut(s) 433, 780, 793, 1126, 1152, 1254
AcsI RAATTY 3 cut(s) 227, 258, 1217
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 3 cut(s) 91, 620, 630
AfiI CCNNNNNNNGG 3 cut(s) 195, 251, 638
AflIII ACRYGT 1 cut(s) 591
AgsI TTSAA 6 cut(s) 224, 313, 480, 848, 923, 1206
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 3 cut(s) 745, 1242, 1254
AjuI GAANNNNNNNTTGG 2 cut(s) 37, 69
AluBI AGCT 6 cut(s) 322, 443, 465, 792, 869, 1034
AluI AGCT 6 cut(s) 322, 443, 465, 792, 869, 1034
AlwI GGATC 6 cut(s) 433, 780, 793, 1126, 1152, 1254
AlwNI CAGNNNCTG 1 cut(s) 680
Aor13HI TCCGGA 1 cut(s) 567
AoxI GGCC 4 cut(s) 276, 349, 484, 607
ApeKI GCWGC 4 cut(s) 141, 166, 185, 1055
ApoI RAATTY 3 cut(s) 227, 258, 1217
Asp700I GAANNNNTTC 1 cut(s) 31
AspS9I GGNCC 3 cut(s) 349, 383, 484
AsuHPI GGTGA 5 cut(s) 266, 329, 377, 560, 1183
AsuNHI GCTAGC 1 cut(s) 332
AvaII GGWCC 1 cut(s) 383
BamHI GGATCC 1 cut(s) 785
BanI GGYRCC 1 cut(s) 818
BauI CACGAG 1 cut(s) 353
BbsI GAAGAC 4 cut(s) 34, 45, 184, 1030
BbvCI CCTCAGC 1 cut(s) 735
BbvI GCAGC 4 cut(s) 153, 153, 197, 1067
BccI CCATC 4 cut(s) 26, 132, 539, 1016
BceAI ACGGC 1 cut(s) 173
BcgI CGANNNNNNTGC 5 cut(s) 35, 646, 680, 815, 849
BciT130I CCWGG 3 cut(s) 747, 1244, 1256
BclI TGATCA 1 cut(s) 112
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 3 cut(s) 87, 333, 1166
BfmI CTRYAG 3 cut(s) 390, 797, 860
BisI GCNGC 6 cut(s) 142, 167, 186, 732, 903, 1056
BlsI GCNGC 6 cut(s) 143, 168, 187, 733, 904, 1057
BmcAI AGTACT 1 cut(s) 620
Bme1390I CCNGG 3 cut(s) 747, 1244, 1256
Bme18I GGWCC 1 cut(s) 383
BmgT120I GGNCC 3 cut(s) 349, 383, 484
BmiI GGNNCC 5 cut(s) 201, 385, 535, 787, 820
BmrFI CCNGG 3 cut(s) 747, 1244, 1256
BmrI ACTGGG 1 cut(s) 783
BmsI GCATC 1 cut(s) 904
BmtI GCTAGC 1 cut(s) 336
BmuI ACTGGG 1 cut(s) 783
BpiI GAAGAC 4 cut(s) 34, 45, 184, 1030
Bpu10I CCTNAGC 1 cut(s) 735
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 4 cut(s) 273, 746, 976, 1255
BsaWI WCCGGW 2 cut(s) 251, 567
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 251, 638
Bse1I ACTGG 4 cut(s) 367, 778, 946, 1194
Bse3DI GCAATG 2 cut(s) 662, 1007
BseAI TCCGGA 1 cut(s) 567
BseBI CCWGG 3 cut(s) 747, 1244, 1256
BseDI CCNNGG 4 cut(s) 273, 746, 976, 1255
BseGI GGATG 1 cut(s) 234
BseLI CCNNNNNNNGG 3 cut(s) 195, 251, 638
BseMI GCAATG 2 cut(s) 662, 1007
BseMII CTCAG 2 cut(s) 606, 726
BseNI ACTGG 4 cut(s) 367, 778, 946, 1194
BseRI GAGGAG 3 cut(s) 148, 151, 1238
BseXI GCAGC 4 cut(s) 153, 153, 197, 1067
BsgI GTGCAG 1 cut(s) 717
Bsh1285I CGRYCG 1 cut(s) 423
BshFI GGCC 4 cut(s) 278, 351, 486, 609
BshNI GGYRCC 1 cut(s) 818
BsiEI CGRYCG 1 cut(s) 423
BsiSI CCGG 3 cut(s) 252, 568, 1059
BslI CCNNNNNNNGG 3 cut(s) 195, 251, 638
BsmI GAATGC 1 cut(s) 1238
BsnI GGCC 4 cut(s) 278, 351, 486, 609
Bsp13I TCCGGA 1 cut(s) 567
BspACI CCGC 4 cut(s) 10, 387, 731, 902
BspANI GGCC 4 cut(s) 278, 351, 486, 609
BspCNI CTCAG 2 cut(s) 607, 727
BspEI TCCGGA 1 cut(s) 567
BspLI GGNNCC 5 cut(s) 201, 385, 535, 787, 820
BspMAI CTGCAG 2 cut(s) 801, 864
BspOI GCTAGC 1 cut(s) 336
BspPI GGATC 6 cut(s) 433, 780, 793, 1126, 1152, 1254
BspT107I GGYRCC 1 cut(s) 818
BsrDI GCAATG 2 cut(s) 662, 1007
BsrI ACTGG 4 cut(s) 367, 778, 946, 1194
BssECI CCNNGG 4 cut(s) 273, 746, 976, 1255
BssNAI GTATAC 1 cut(s) 589
BssNI GRCGYC 1 cut(s) 95
BssSI CACGAG 1 cut(s) 353
BssT1I CCWWGG 1 cut(s) 273
Bst1107I GTATAC 1 cut(s) 589
Bst2BI CACGAG 1 cut(s) 353
Bst2UI CCWGG 3 cut(s) 747, 1244, 1256
Bst4CI ACNGT 3 cut(s) 524, 623, 1088
Bst6I CTCTTC 1 cut(s) 125
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 818
BstC8I GCNNGC 2 cut(s) 334, 1007
BstDEI CTNAG 3 cut(s) 615, 735, 870
BstF5I GGATG 1 cut(s) 234
BstMCI CGRYCG 1 cut(s) 423
BstMWI GCNNNNNNNGC 6 cut(s) 716, 731, 818, 971, 1006, 1061
BstNI CCWGG 3 cut(s) 747, 1244, 1256
BstNSI RCATGY 2 cut(s) 595, 731
BstSCI CCNGG 3 cut(s) 745, 1242, 1254
BstSFI CTRYAG 3 cut(s) 390, 797, 860
BstV1I GCAGC 4 cut(s) 153, 153, 197, 1067
BstV2I GAAGAC 4 cut(s) 34, 45, 184, 1030
BstX2I RGATCY 4 cut(s) 425, 785, 1144, 1246
BstYI RGATCY 4 cut(s) 425, 785, 1144, 1246
BstZ17I GTATAC 1 cut(s) 589
BsuRI GGCC 4 cut(s) 278, 351, 486, 609
BtgZI GCGATG 1 cut(s) 4
BtsCI GGATG 1 cut(s) 234
BtsI GCAGTG 4 cut(s) 19, 580, 1233, 1284
BtsIMutI CAGTG 5 cut(s) 19, 580, 1201, 1233, 1284
Cac8I GCNNGC 2 cut(s) 334, 1007
CaiI CAGNNNCTG 1 cut(s) 680
Cfr13I GGNCC 3 cut(s) 349, 383, 484
Csp6I GTAC 3 cut(s) 90, 619, 629
CspCI CAANNNNNGTGG 2 cut(s) 262, 297
CviAII CATG 7 cut(s) 178, 305, 380, 451, 592, 728, 890
CviQI GTAC 3 cut(s) 90, 619, 629
DdeI CTNAG 3 cut(s) 615, 735, 870
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Eco130I CCWWGG 1 cut(s) 273
Eco147I AGGCCT 2 cut(s) 278, 609
Eco47I GGWCC 1 cut(s) 383
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 3 cut(s) 745, 1242, 1254
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 7 cut(s) 181, 308, 383, 454, 595, 731, 893
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FatI CATG 7 cut(s) 177, 304, 379, 450, 591, 727, 889
FauI CCCGC 1 cut(s) 394
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 1 cut(s) 588
Fnu4HI GCNGC 6 cut(s) 142, 167, 186, 732, 903, 1056
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 6 cut(s) 142, 167, 186, 732, 903, 1056
FspBI CTAG 3 cut(s) 87, 333, 1166
GluI GCNGC 6 cut(s) 142, 167, 186, 732, 903, 1056
HaeIII GGCC 4 cut(s) 278, 351, 486, 609
HapII CCGG 3 cut(s) 252, 568, 1059
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 7 cut(s) 181, 308, 383, 454, 595, 731, 893
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HinfI GANTC 4 cut(s) 285, 677, 752, 1226
HpaII CCGG 3 cut(s) 252, 568, 1059
HphI GGTGA 5 cut(s) 266, 329, 377, 560, 1183
Hpy166II GTNNAC 3 cut(s) 75, 589, 958
Hpy188I TCNGA 6 cut(s) 129, 298, 425, 826, 980, 1231
Hpy188III TCNNGA 3 cut(s) 568, 848, 1206
Hpy8I GTNNAC 3 cut(s) 75, 589, 958
Hpy99I CGWCG 4 cut(s) 97, 100, 377, 494
HpyAV CCTTC 3 cut(s) 180, 307, 1125
HpyCH4III ACNGT 3 cut(s) 524, 623, 1088
HpyCH4IV ACGT 2 cut(s) 95, 960
HpyCH4V TGCA 7 cut(s) 698, 799, 836, 862, 1000, 1005, 1238
HpyF10VI GCNNNNNNNGC 6 cut(s) 716, 731, 818, 971, 1006, 1061
HpyF3I CTNAG 3 cut(s) 615, 735, 870
HpySE526I ACGT 2 cut(s) 95, 960
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 7 cut(s) 181, 308, 383, 454, 595, 731, 893
Kpn2I TCCGGA 1 cut(s) 567
Ksp22I TGATCA 1 cut(s) 112
LmnI GCTCC 4 cut(s) 138, 440, 570, 1061
Lsp1109I GCAGC 4 cut(s) 153, 153, 197, 1067
LweI GCATC 1 cut(s) 904
MaeI CTAG 3 cut(s) 87, 333, 1166
MaeII ACGT 2 cut(s) 95, 960
MfeI CAATTG 1 cut(s) 1065
MflI RGATCY 4 cut(s) 425, 785, 1144, 1246
MluCI AATT 7 cut(s) 227, 258, 926, 1065, 1113, 1217, 1263
MlyI GAGTC 3 cut(s) 294, 686, 1235
MmeI TCCRAC 3 cut(s) 418, 763, 849
MroI TCCGGA 1 cut(s) 567
MroXI GAANNNNTTC 1 cut(s) 31
MseI TTAA 1 cut(s) 1083
MspA1I CMGCKG 1 cut(s) 792
MspI CCGG 3 cut(s) 252, 568, 1059
MspR9I CCNGG 3 cut(s) 747, 1244, 1256
MunI CAATTG 1 cut(s) 1065
Mva1269I GAATGC 1 cut(s) 1238
MvaI CCWGG 3 cut(s) 747, 1244, 1256
MwoI GCNNNNNNNGC 6 cut(s) 716, 731, 818, 971, 1006, 1061
NheI GCTAGC 1 cut(s) 332
NlaIII CATG 7 cut(s) 181, 308, 383, 454, 595, 731, 893
NlaIV GGNNCC 5 cut(s) 201, 385, 535, 787, 820
NspI RCATGY 2 cut(s) 595, 731
PceI AGGCCT 2 cut(s) 278, 609
PciI ACATGT 1 cut(s) 591
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PctI GAATGC 1 cut(s) 1238
PdmI GAANNNNTTC 1 cut(s) 31
PfeI GAWTC 1 cut(s) 752
PflMI CCANNNNNTGG 1 cut(s) 638
PfoI TCCNGGA 1 cut(s) 1242
PkrI GCNGC 6 cut(s) 143, 168, 187, 733, 904, 1057
Ple19I CGATCG 1 cut(s) 423
PleI GAGTC 3 cut(s) 293, 685, 1234
PpsI GAGTC 3 cut(s) 293, 685, 1234
PscI ACATGT 1 cut(s) 591
PsiI TTATAA 1 cut(s) 896
Psp6I CCWGG 3 cut(s) 745, 1242, 1254
PspGI CCWGG 3 cut(s) 745, 1242, 1254
PspN4I GGNNCC 5 cut(s) 201, 385, 535, 787, 820
PspPI GGNCC 3 cut(s) 349, 383, 484
PstI CTGCAG 2 cut(s) 801, 864
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 4 cut(s) 425, 785, 1144, 1246
PvuI CGATCG 1 cut(s) 423
PvuII CAGCTG 1 cut(s) 792
RsaI GTAC 3 cut(s) 91, 620, 630
RsaNI GTAC 3 cut(s) 90, 619, 629
SaqAI TTAA 1 cut(s) 1083
SatI GCNGC 6 cut(s) 142, 167, 186, 732, 903, 1056
Sau96I GGNCC 3 cut(s) 349, 383, 484
ScaI AGTACT 1 cut(s) 620
SchI GAGTC 3 cut(s) 294, 686, 1235
ScrFI CCNGG 3 cut(s) 747, 1244, 1256
SfaNI GCATC 1 cut(s) 904
SfcI CTRYAG 3 cut(s) 390, 797, 860
SinI GGWCC 1 cut(s) 383
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 7 cut(s) 227, 258, 926, 1065, 1113, 1217, 1263
SseBI AGGCCT 2 cut(s) 278, 609
SsiI CCGC 4 cut(s) 10, 387, 731, 902
SspMI CTAG 3 cut(s) 87, 333, 1166
StuI AGGCCT 2 cut(s) 278, 609
StyD4I CCNGG 3 cut(s) 745, 1242, 1254
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 3 cut(s) 524, 623, 1088
TaiI ACGT 2 cut(s) 98, 963
TaqI TCGA 3 cut(s) 375, 840, 1221
TasI AATT 7 cut(s) 227, 258, 926, 1065, 1113, 1217, 1263
TatI WGTACW 1 cut(s) 618
TauI GCSGC 2 cut(s) 734, 905
TfiI GAWTC 1 cut(s) 752
Tru1I TTAA 1 cut(s) 1083
Tru9I TTAA 1 cut(s) 1083
TscAI CASTG 5 cut(s) 19, 580, 1201, 1240, 1284
TseI GCWGC 4 cut(s) 141, 166, 185, 1055
TspDTI ATGAA 2 cut(s) 45, 1030
TspGWI ACGGA 1 cut(s) 1140
TspRI CASTG 5 cut(s) 19, 580, 1201, 1240, 1284
Van91I CCANNNNNTGG 1 cut(s) 638
VpaK11BI GGWCC 1 cut(s) 383
XapI RAATTY 3 cut(s) 227, 258, 1217
XceI RCATGY 2 cut(s) 595, 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 359
XmiI GTMKAC 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 31
XspI CTAG 3 cut(s) 87, 333, 1166
ZraI GACGTC 1 cut(s) 96
ZrmI AGTACT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.