Rmu_ssc0000116.1_g000009

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000116.1
OG0010532
Physical Location & Seq
Forward (+)
43002 .. 52908
9907 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 7014 bp
atgaggacgaggacgagcaacagacgagacgaggatgagcgccgtttgtgtttctactttctaggatggagactgagagagcgggtgttgactcttgagcttgctagtaaatccaacaagaacaagaagcaagccttccagttccaatcagaaaaccgaatttcaacttcatttctcgcattagcctacttgatcaaataccccagcttcgatcttgtgctagcttgttcatcaatggctgctgctgattcattcggtagccgcagttggaaatacgatgtgttcctgagttttagagggccagacactcgcaagatcttcgtaggccatcttcacagagctctggaacggaaatcactcaccgccttcatagacactgaagagctcccaaaaggtgaagtcattcaggtgctgctcaaggctattgaggactcaaagctttcggttgtagttttctctcaaaactacgcttcttcaagatggtgcttgaaagaactggaccaaattgtagaatgcatggatacaaagaagcaaatagtggtgccggttttttacgaagtagatccggccaatgtccgttatcagaagggaaaattcgaggatgcttttgccaaatatgtgaaagatggtagggaaagcgaagaagtgttggagaggtggaaatctgctctaaaaagagccgctgatttgtctggctgggattcacgctcatatggggatgatgccgagcttattgagcttgttgttgaagatatttttgaaaaattgataaagacctggtcaagtgaaagtgaatccaatcacttggttggaatggaatgccatattcagaaaatggaagtcctattaagtcctggggggaatgatgtccgcactgttggcatatggggtatgggcggtttaggcaagacaacaatcgctagagctgtttacgacaaattcactagtaactttgatgcttgttgctttcttgaaaatgtcaggcataatttcaaggaaaaaggtgaaggacaaatgcaggaagtgcttttatccaaaatcttgaaggaaaaggtgcggactttaggcacttcgaatatggcttctgagatgataatggaaatgcttaggaagaaaaaacttttacttgttctcgatgatgtggacaaatcagctcaaattgaaggcttacttggaaagaatcgctcatttggttgtgggagtagaatcattataacaactagagaccagcacttgatgagtgaatatttgaactacgatcctgggtatctaggagatgaagaagctcttgaactctttagagagtatgctaaaccttcagaacagcataattatcttctaagcaatgccgtaggatacgcccaagggctccctttagcaattaaagtattgggagcttctcttcgtgacaaaagtatcggtgagtgggaagatgagcttgagaaaataaagaaattccagcacccggacattgaaaatgtgctgatgataagctttgaaggactagatgatccacaaaagacaatatttctggatgttgcatgtttctttaaaggactcaaaaaggactatgcaacaataattctggaagcttgcgacttgtttcccacaagtggattaagagttctagtcgatagagctcttgtaactacatctggataccactctgaggtactggggatgcatgatttactacaagacatggctttgggaattgtgcgtcgagaatcgagaccacccagtaggttgaccacttctgataagatcgtggagatgttaactgagaatacggggatggaagtagttgaaagcatcgacctagacttgtccggctcaaaagacgtggtcataaatattgaagctttcgttaatatgaaaagactaagactgctcagaattcattataaccgctctatagactctgttgatgattattacagttcaagtgaggaattatacaatccaagtgaggagtgcaaacaacacgtgagtggggacttaaagtctctttctcagaggttgaggtgtctcatttggcatggatgccccttgaagtctttgccatcacattttttcccaatgcatcttgtcgacattgacatgcgttgcagcagtatcacaaaactttgggaaggaatcaagcctcttggaaaattgaaattcatcaatttaagtcgatctccataccttgaagaattccccaatttaacaaaagcaataaatcttgagaaagtaaaccttgaaggttgtataagtttatcgaaggttgactcgtccatttcagaacttaaaaaccttgttttcttgaatctaaaagggtgcaaagaacttaagattctttcaagccacatcggtatgaaacgtcttaagacctttgagctttctggttgctcaaatcttgagaactttcctgagatttcagaagttatggaggagctaccacggctttgtctggatatgactgcaattaaaagactgcctccatcaattaaaaatcttaagggacttgtcactttgagcctaaaagattgcagagaacttgagagtctcccaagcagcatgtgccaactcaagtccttaagatatgttactctctctgggtgttctaagtttaaggtgtttccggaaattgtgggggcagaagatatggaaaaactaaaagagcttcatttggatggaacatctatcaaagagtttttgccgtcaacttcagctcttcgaaatcttgaagtcttgagtctaagagattgcaaggagcttgagactcttccaagtagcattcatatggtatctcttgaatcactttttctctctggctgctcaaaccttaagaagtttccaaagattttaccgagtatgcgctacctatcagagcttcatttagacaacattgcaattgaagaactgcccccgtcaataaaaaatctttgcaatctgagaactttgatccttagatattgtggagaaattaaaagtcttccgagcagcatttttcaactcatgtatcttaaagaaattactctctctggttgttcaaagtttgaggtgtttccagaatttgttgaagagctttgttgggataaaacatctatcgggaggttttcccttgttactctgaggctagaagattgcggagaacttaagagtctgccgagcagcttttttctactcaagtcccttagattcgttacgctttctggttgttcaaagtttgaggtgtttccagaaatattagaagatatggaacaattaagagagcttcatttggatggaacgtctatcaaaggcctttcctcatcaattgaacggcttaagaggattatgttattgaacatgagtaactgcagaagcctagtctctcttcctgacaatatctgtaatttgtcataccttacaggtctcaatctctcagggtgcttgaatctccataacctgcccgagaacttggggagattagaattcttgctggatcttgaagtacagggaagtggtataaagcaaccattctctcccttacatctgatgaggtcaccatcatcagcaaccaattgtttggtcggaatggatcgccacataaatcaaatgcagacgctattatgtcctcttcaggtggatgttgttcttactgttggaatatttggaaggtgtggtattggcaaaacaaccattgctagtgctgtttatgatgaaattgctcctcaatttgaacactgttgctttcttgagaatatcaacgaaggtttcacgaagcatggggcagtatatatgcaagcaaaacttctatcaggaatgttgaaggaaaagatgcagagtttaggcaatttgaatgcaggttacaatatgatgatggaaaaactaggtatgaaaaaagttctagtcgtttttgatgatgtacataatactacccagattgaagccttacttggaaaaccatattcatttggttgtgggagtagaatcattataacaactagaaaggaaacaataatgagtggatgtcagatatattgtcccaatctattagatgatgaagctgttaaactcttcaggcattttgctttcagaacaatcaattgctcagatgagtatgatcttctctcacggcgtgccattggatgtgctcaacgtctgcctttagcacttaaagtcttaggatcttttctttttgacaaaagtatcgaagagtgggaagttgccttgaaaggactagaaagattctctagcagaggagttgaaagtgtgttttttaaaagctttgatgacctacatgattcagagaagaccatatttcttgatgttgcatgtttctttaagggaaggaaaaaagactatgtaacagaaattctgcttagttgtggcttctttccctatagtggattaagagttctaattgatagatctctcttaactatctcacattgggacgggactctcaaaatgtacgattcaatacacgaagttggtcgtgaaagggcccgcctagaatctcttcacggtcctggggaacgcagcaggttatggagtaatgatgggatttattgtttgtcatctgaaaatcgggttaccaatgcggttgaagctataaaccttgatttgtccaactcaaaagaggtaaacttaagcgcaaagtcttttgttgaaatgaagaaactaagactgctccaaatccttgatgacttcccaggtcgtaaacaacttgtgagtgaaaacttcgagtttctttctcatgagttgaggtgtctcatctggcatagatgccctctgaggtctttgccatccaattttcacccctggaatcttgttgagctagacatgcgtggtagtctcattgaacacctctgtgaaggaaccaagcttcttagaaacttgaaattcatcaatttaagtcactgtccacgtcttgagaagatcccagacttgacggaagctacgaatcttgagaagctaattcttgatggtagtacaattttattggaggtgcacccatccatttggaatcataagaaccttgtcttgttaagtctagaaggctgcagagaacttaagattcatccggccagcatccatatgaattctctcaagaccctcaacctttctggctgcttaaaccttgagaagtttcccgagatttcagaagttatgatggagctatcagagcttcgtttagatgagactgcaattaaaaaactaccctcttcaattgaacggctacaggggcttaatgtattaagtatgagaaactgcacaagccttgtctctcttccggacagcatctgtagtttggcacaccttacatttctcaatctgtctgggtgcccaaaacttactgactttcctgactgcttgaggaatatgcaatcattgacaatcagtggtatagaacaactcccacccgcaatgagaagcaaaagaacagagagggaggaagtcattggagtctcgggggagggaagttcatcagagttgcaagctatagcagtcagcagacattcagttctcttctgccttcgcgactcgtctaactcccaaaacgtggcaaatcatgaaactttgtccagcaaatggagagttggagttggagttggatttgcagcaaaagatttaaggtgtgaatttgatttcctcgatcttgctgatgaatttgatgctttgaatgatgaatatcttgaattcgatgctttgaatgatgaatatcttgaaaaggtgcagagtttaggcaatttgaatgcaggttacaatatgatgatggaaaaactagggaaaatgactgtaacagaaattctgcttagttgtggcttctttccctatagtggattaagagttctaattgatagatctctcttaactatctcacattgggacgggactctcaaaatgtacgattcaatacacgaagttggtcgtgaaagggcccgcctagaatctcttcacggtcctggggaacgcagcaggttatggagtaatgatgggatttattgtttgtcatctgaaaatcggagttttttggttattcaggttaccaatgcggttgaagctataaaccttgatttgtccaactcaaaagaggtaaacttaagcgcaaagtcttttgttgaaatgaagaaactaagactgctccaaatccttgatgacttcccaggtcgtaaacaacttgtgagtgaaaacttcgagtttctttctcatgagttgaggtgtctcatctggcatagatgccctctgaggtctttgccatccaattttcacccctggaatcttgttgagctagacatgcgtggtagtctcattgaacacctctgtgaaggaaccaagcttcttagaaacttgaaattcatcaatttaagtcactgtccatatcttaagaacactcctgacttgactgaggctacgaagcttgagaagctgattcttgaaagttgtacaagtttattcgaggtgcacccatccattttcgatcggagaaatcttgttttcttaaatctaaaaggttgcagagaacttaagattcttccgagcagcatccatatgaaatctcttaagacacttcaactttttggctgctcaaatcttcagaattttcccaacatttcagaagttatgatggagctatcagaaattaatttggatgagactgcaattaaagaactgccctcatcaattgaactgcttcatgggcttaatgtattaagtatgagagactgcacaagccttgtctctcttccggatagtatctataatttgcaataccttcaacagcttcatctggatgggtgctcaaaactttcaaattttcctgaaaacttgaggaatgcacaatcattgacaatcagtggtatggaacaactcccattttttatgagagacaacgaaacagagagggagggagtcattggagtcaccagggaaacaatggaggcagcaatgcatggggaggagtgctcggcaggaggaggaatgttagggtcctcatgcagctgcttgagttggctaggtgaactatgtgatcgaggaagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

2337

Amino Acids

264.81

Weight (kDa)

6.0

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 91 - 271 2e-50 TIR domain
TIR_2 PF13676 94 - 190 3.8e-15 TIR domain
NB-ARC PF00931 281 - 440 2.7e-20 NB-ARC domain
WHD_ROQ1 PF23282 506 - 577 2.8e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 695 - 714 8e-06 Leucine Rich Repeat
LRR_13 PF23286 789 - 871 2e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 789 - 915 8.8e-10 Leucine-rich repeat region
LRR_13 PF23286 930 - 1022 5.2e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 935 - 1020 2.6e-07 Leucine-rich repeat region
LRR_14 PF23598 1039 - 1161 4.3e-07 Leucine-rich repeat region
LRR_13 PF23286 1057 - 1143 3.5e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
NB-ARC PF00931 1192 - 1355 1.2e-12 NB-ARC domain
WHD_ROQ1 PF23282 1425 - 1493 1.4e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 1685 - 1789 5e-11 Leucine-rich repeat region
LRR_13 PF23286 1690 - 1777 9.7e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 2167 - 2276 2.5e-09 Leucine-rich repeat region
LRR_13 PF23286 2175 - 2262 1.2e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1214, 1905, 3965
Acc36I ACCTGC 5 cut(s) 3441, 3821, 4503, 5720, 5940
AccB1I GGYRCC 2 cut(s) 541, 5313
AccB7I CCANNNNNTGG 2 cut(s) 5533, 5562
AccBSI CCGCTC 2 cut(s) 82, 1911
AccI GTMKAC 1 cut(s) 2091
AccII CGCG 1 cut(s) 5511
AccIII TCCGGA 3 cut(s) 2623, 5263, 6718
AclWI GGATC 8 cut(s) 557, 1253, 1505, 2941, 3477, 3573, 4162, 4903
AcoI YGGCCR 2 cut(s) 567, 5055
AcuI CTGAAG 6 cut(s) 399, 1302, 2694, 3590, 4030, 6551
AcvI CACGTG 1 cut(s) 1987
AdeI CACNNNGTG 1 cut(s) 4106
AfaI GTAC 7 cut(s) 1674, 3480, 3894, 4442, 4963, 5879, 6418
AfiI CCNNNNNNNGG 7 cut(s) 1465, 1613, 1669, 4373, 5533, 5562, 5810
AflIII ACRYGT 1 cut(s) 1984
AhdI GACNNNNNGTC 1 cut(s) 2002
AhlI ACTAGT 1 cut(s) 944
AjiI CACGTC 2 cut(s) 1843, 4898
AjuI GAANNNNNNNTTGG 4 cut(s) 1155, 1187, 3906, 3938
AleI CACNNNNGTG 3 cut(s) 1989, 4839, 6294
AloI GAACNNNNNNTCC 6 cut(s) 1608, 1640, 4368, 4400, 5805, 5837
Alw21I GWGCWC 8 cut(s) 343, 387, 1642, 4123, 4983, 6438, 6773, 6938
Alw44I GTGCAC 2 cut(s) 4979, 6434
AlwI GGATC 8 cut(s) 557, 1253, 1505, 2941, 3477, 3573, 4162, 4903
AlwNI CAGNNNCTG 2 cut(s) 412, 5274
Ama87I CYCGRG 3 cut(s) 3437, 5123, 5440
Aor13HI TCCGGA 3 cut(s) 2623, 5263, 6718
AoxI GGCC 7 cut(s) 299, 325, 567, 3286, 4473, 5055, 5910
ApaI GGGCCC 2 cut(s) 4477, 5914
ApaLI GTGCAC 2 cut(s) 4979, 6434
AseI ATTAAT 1 cut(s) 6615
Asp700I GAANNNNTTC 4 cut(s) 402, 4488, 5925, 6663
AspLEI GCGC 4 cut(s) 42, 2862, 4625, 6080
AspS9I GGNCC 9 cut(s) 299, 499, 4473, 4474, 4496, 5910, 5911, 5933, 6960
AsuC2I CCSGG 1 cut(s) 1466
AsuHPI GGTGA 9 cut(s) 352, 407, 1016, 1433, 3522, 4778, 6233, 6886, 7001
AsuII TTCGAA 2 cut(s) 1073, 2719
AsuNHI GCTAGC 1 cut(s) 220
AvaI CYCGRG 3 cut(s) 3437, 5123, 5440
AvaII GGWCC 4 cut(s) 499, 4496, 5933, 6960
BaeGI GKGCMC 5 cut(s) 4477, 4983, 5318, 5914, 6438
BaeI ACNNNNGTAYC 6 cut(s) 1399, 1432, 2987, 3020, 4159, 4192
BanI GGYRCC 2 cut(s) 541, 5313
BanII GRGCYC 6 cut(s) 343, 387, 1371, 1642, 4477, 5914
BarI GAAGNNNNNNTAC 2 cut(s) 315, 347
BbrPI CACGTG 1 cut(s) 1987
BbsI GAAGAC 2 cut(s) 2969, 4286
Bbv12I GWGCWC 8 cut(s) 343, 387, 1642, 4123, 4983, 6438, 6773, 6938
BceAI ACGGC 7 cut(s) 27, 1334, 2459, 2686, 3323, 4118, 5222
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciVI GTATCC 3 cut(s) 514, 1349, 1652
BclI TGATCA 1 cut(s) 192
BcnI CCSGG 1 cut(s) 1466
BcuI ACTAGT 1 cut(s) 944
BfmI CTRYAG 8 cut(s) 1914, 3343, 4369, 5032, 5210, 5275, 5472, 5806
BfoI RGCGCY 1 cut(s) 43
BfuAI ACCTGC 5 cut(s) 3441, 3821, 4503, 5720, 5940
BfuI GTATCC 3 cut(s) 514, 1349, 1652
BglII AGATCT 3 cut(s) 315, 4397, 5834
Bme18I GGWCC 4 cut(s) 499, 4496, 5933, 6960
BmeRI GACNNNNNGTC 1 cut(s) 2002
BmeT110I CYCGRG 3 cut(s) 3437, 5123, 5440
BmgBI CACGTC 2 cut(s) 1843, 4898
BmgT120I GGNCC 9 cut(s) 299, 499, 4473, 4474, 4496, 5910, 5911, 5933, 6960
BmiI GGNNCC 8 cut(s) 543, 1370, 4475, 4849, 5315, 5912, 6304, 6961
BmrI ACTGGG 2 cut(s) 1685, 1734
BmtI GCTAGC 1 cut(s) 224
BmuI ACTGGG 2 cut(s) 1685, 1734
BpiI GAAGAC 2 cut(s) 2969, 4286
BplI GAGNNNNNCTC 2 cut(s) 6920, 6952
Bpu10I CCTNAGC 1 cut(s) 1106
Bpu14I TTCGAA 2 cut(s) 1073, 2719
BpuMI CCSGG 1 cut(s) 1466
BsaAI YACGTR 1 cut(s) 1987
BsaBI GATNNNNATC 2 cut(s) 5661, 5691
BsaI GGTCTC 3 cut(s) 1218, 1726, 3404
BsaWI WCCGGW 3 cut(s) 2623, 5263, 6718
BsaXI ACNNNNNCTCC 2 cut(s) 5565, 5595
Bsc4I CCNNNNNNNGG 7 cut(s) 1465, 1613, 1669, 4373, 5533, 5562, 5810
Bse118I RCCGGY 1 cut(s) 544
Bse1I ACTGG 4 cut(s) 139, 501, 1680, 1740
Bse3DI GCAATG 5 cut(s) 1351, 2889, 3666, 5403, 6924
Bse8I GATNNNNATC 2 cut(s) 5661, 5691
BseAI TCCGGA 3 cut(s) 2623, 5263, 6718
BseJI GATNNNNATC 2 cut(s) 5661, 5691
BseLI CCNNNNNNNGG 7 cut(s) 1465, 1613, 1669, 4373, 5533, 5562, 5810
BseMI GCAATG 5 cut(s) 1351, 2889, 3666, 5403, 6924
BseNI ACTGG 4 cut(s) 139, 501, 1680, 1740
BseRI GAGGAG 6 cut(s) 1985, 2447, 3687, 4242, 6944, 6960
BseSI GKGCMC 5 cut(s) 4477, 4983, 5318, 5914, 6438
BseYI CCCAGC 2 cut(s) 203, 696
BsgI GTGCAG 3 cut(s) 5227, 5726, 6682
Bsh1236I CGCG 1 cut(s) 5511
Bsh1285I CGRYCG 1 cut(s) 6454
BshFI GGCC 7 cut(s) 301, 327, 569, 3288, 4475, 5057, 5912
BshNI GGYRCC 2 cut(s) 541, 5313
BsiEI CGRYCG 1 cut(s) 6454
BsiHKAI GWGCWC 8 cut(s) 343, 387, 1642, 4123, 4983, 6438, 6773, 6938
BsiHKCI CYCGRG 3 cut(s) 3437, 5123, 5440
BsiSI CCGG 8 cut(s) 545, 566, 1466, 1830, 2624, 5054, 5264, 6719
BslFI GGGAC 8 cut(s) 2009, 2517, 3160, 3996, 4436, 4441, 5873, 5878
BslI CCNNNNNNNGG 7 cut(s) 1465, 1613, 1669, 4373, 5533, 5562, 5810
BsmBI CGTCTC 1 cut(s) 21
BsmFI GGGAC 8 cut(s) 2009, 2517, 3160, 3996, 4436, 4441, 5873, 5878
BsmI GAATGC 6 cut(s) 518, 824, 2778, 3832, 5731, 6811
BsnI GGCC 7 cut(s) 301, 327, 569, 3288, 4475, 5057, 5912
Bso31I GGTCTC 3 cut(s) 1218, 1726, 3404
BsoBI CYCGRG 3 cut(s) 3437, 5123, 5440
Bsp119I TTCGAA 2 cut(s) 1073, 2719
Bsp120I GGGCCC 2 cut(s) 4473, 5910
Bsp13I TCCGGA 3 cut(s) 2623, 5263, 6718
Bsp1407I TGTACA 2 cut(s) 3892, 6416
Bsp68I TCGCGA 1 cut(s) 5511
BspANI GGCC 7 cut(s) 301, 327, 569, 3288, 4475, 5057, 5912
BspEI TCCGGA 3 cut(s) 2623, 5263, 6718
BspFNI CGCG 1 cut(s) 5511
BspHI TCATGA 3 cut(s) 4726, 5542, 6181
BspLI GGNNCC 8 cut(s) 543, 1370, 4475, 4849, 5315, 5912, 6304, 6961
BspMAI CTGCAG 2 cut(s) 3347, 5036
BspMI ACCTGC 5 cut(s) 3441, 3821, 4503, 5720, 5940
BspOI GCTAGC 1 cut(s) 224
BspPI GGATC 8 cut(s) 557, 1253, 1505, 2941, 3477, 3573, 4162, 4903
BspQI GCTCTTC 3 cut(s) 375, 2721, 3060
BspT104I TTCGAA 2 cut(s) 1073, 2719
BspT107I GGYRCC 2 cut(s) 541, 5313
BspTNI GGTCTC 3 cut(s) 1218, 1726, 3404
BsrBI CCGCTC 2 cut(s) 82, 1911
BsrDI GCAATG 5 cut(s) 1351, 2889, 3666, 5403, 6924
BsrFI RCCGGY 1 cut(s) 544
BsrGI TGTACA 2 cut(s) 3892, 6416
BsrI ACTGG 4 cut(s) 139, 501, 1680, 1740
BssAI RCCGGY 1 cut(s) 544
BssT1I CCWWGG 1 cut(s) 1363
Bst4CI ACNGT 9 cut(s) 877, 1940, 3628, 3713, 4496, 4892, 5770, 5933, 6347
BstAPI GCANNNNNTGC 2 cut(s) 1024, 2562
BstAUI TGTACA 2 cut(s) 3892, 6416
BstBAI YACGTR 1 cut(s) 1987
BstBI TTCGAA 2 cut(s) 1073, 2719
BstDSI CCRYGG 1 cut(s) 2441
BstEII GGTNACC 3 cut(s) 3528, 4561, 6016
BstFNI CGCG 1 cut(s) 5511
BstH2I RGCGCY 1 cut(s) 43
BstHHI GCGC 4 cut(s) 42, 2862, 4625, 6080
BstMCI CGRYCG 1 cut(s) 6454
BstNSI RCATGY 6 cut(s) 1545, 2104, 2563, 4305, 4816, 6271
BstPI GGTNACC 3 cut(s) 3528, 4561, 6016
BstSFI CTRYAG 8 cut(s) 1914, 3343, 4369, 5032, 5210, 5275, 5472, 5806
BstSLI GKGCMC 5 cut(s) 4477, 4983, 5318, 5914, 6438
BstUI CGCG 1 cut(s) 5511
BstV2I GAAGAC 2 cut(s) 2969, 4286
BstX2I RGATCY 7 cut(s) 315, 562, 3469, 4154, 4397, 4908, 5834
BstXI CCANNNNNNTGG 3 cut(s) 805, 3553, 4992
BstYI RGATCY 7 cut(s) 315, 562, 3469, 4154, 4397, 4908, 5834
BsuI GTATCC 3 cut(s) 514, 1349, 1652
BsuRI GGCC 7 cut(s) 301, 327, 569, 3288, 4475, 5057, 5912
BtgI CCRYGG 1 cut(s) 2441
BtrI CACGTC 2 cut(s) 1843, 4898
BtsIMutI CAGTG 7 cut(s) 375, 873, 3709, 4888, 5377, 6343, 6832
BtuMI TCGCGA 1 cut(s) 5511
BveI ACCTGC 5 cut(s) 3441, 3821, 4503, 5720, 5940
CaiI CAGNNNCTG 2 cut(s) 412, 5274
CciI TCATGA 3 cut(s) 4726, 5542, 6181
CfoI GCGC 4 cut(s) 42, 2862, 4625, 6080
Cfr10I RCCGGY 1 cut(s) 544
Cfr13I GGNCC 9 cut(s) 299, 499, 4473, 4474, 4496, 5910, 5911, 5933, 6960
CseI GACGC 2 cut(s) 1709, 3598
CsiI ACCWGGT 1 cut(s) 776
Csp6I GTAC 7 cut(s) 1673, 3479, 3893, 4441, 4962, 5878, 6417
CspCI CAANNNNNGTGG 2 cut(s) 1824, 1859
CviQI GTAC 7 cut(s) 1673, 3479, 3893, 4441, 4962, 5878, 6417
DraI TTTAAA 2 cut(s) 1552, 4249
DraIII CACNNNGTG 1 cut(s) 4106
DriI GACNNNNNGTC 1 cut(s) 2002
EaeI YGGCCR 2 cut(s) 567, 5055
Eam1105I GACNNNNNGTC 1 cut(s) 2002
Ecl136II GAGCTC 3 cut(s) 341, 385, 1640
Eco130I CCWWGG 1 cut(s) 1363
Eco147I AGGCCT 1 cut(s) 3288
Eco24I GRGCYC 6 cut(s) 343, 387, 1371, 1642, 4477, 5914
Eco31I GGTCTC 3 cut(s) 1218, 1726, 3404
Eco47I GGWCC 4 cut(s) 499, 4496, 5933, 6960
Eco53kI GAGCTC 3 cut(s) 341, 385, 1640
Eco57I CTGAAG 6 cut(s) 399, 1302, 2694, 3590, 4030, 6551
Eco72I CACGTG 1 cut(s) 1987
Eco88I CYCGRG 3 cut(s) 3437, 5123, 5440
Eco91I GGTNACC 3 cut(s) 3528, 4561, 6016
EcoICRI GAGCTC 3 cut(s) 341, 385, 1640
EcoO109I RGGNCCY 3 cut(s) 4473, 5910, 6960
EcoO65I GGTNACC 3 cut(s) 3528, 4561, 6016
EcoRI GAATTC 5 cut(s) 1896, 2195, 3458, 5071, 5669
EcoT14I CCWWGG 1 cut(s) 1363
EcoT22I ATGCAT 4 cut(s) 518, 1686, 2085, 6924
EcoT38I GRGCYC 6 cut(s) 343, 387, 1371, 1642, 4477, 5914
ErhI CCWWGG 1 cut(s) 1363
Esp3I CGTCTC 1 cut(s) 21
FaqI GGGAC 8 cut(s) 2009, 2517, 3160, 3996, 4436, 4441, 5873, 5878
FauI CCCGC 4 cut(s) 75, 4484, 5401, 5921
FauNDI CATATG 5 cut(s) 712, 884, 2784, 5067, 6522
FbaI TGATCA 1 cut(s) 192
FblI GTMKAC 1 cut(s) 2091
FriOI GRGCYC 6 cut(s) 343, 387, 1371, 1642, 4477, 5914
GlaI GCGC 4 cut(s) 41, 2861, 4624, 6079
GsaI CCCAGC 2 cut(s) 207, 700
HaeII RGCGCY 1 cut(s) 43
HaeIII GGCC 7 cut(s) 301, 327, 569, 3288, 4475, 5057, 5912
HapII CCGG 8 cut(s) 545, 566, 1466, 1830, 2624, 5054, 5264, 6719
HgaI GACGC 2 cut(s) 1709, 3598
HhaI GCGC 4 cut(s) 42, 2862, 4625, 6080
Hin6I GCGC 4 cut(s) 40, 2860, 4623, 6078
HinP1I GCGC 4 cut(s) 40, 2860, 4623, 6078
HincII GTYRAC 6 cut(s) 90, 1749, 1779, 2092, 2269, 2706
HindII GTYRAC 6 cut(s) 90, 1749, 1779, 2092, 2269, 2706
HindIII AAGCTT 8 cut(s) 437, 1492, 1590, 1860, 4252, 4853, 6308, 6389
HpaI GTTAAC 1 cut(s) 1779
HpaII CCGG 8 cut(s) 545, 566, 1466, 1830, 2624, 5054, 5264, 6719
HphI GGTGA 9 cut(s) 352, 407, 1016, 1433, 3522, 4778, 6233, 6886, 7001
Hpy99I CGWCG 1 cut(s) 1725
HpyCH4III ACNGT 9 cut(s) 877, 1940, 3628, 3713, 4496, 4892, 5770, 5933, 6347
HpyCH4IV ACGT 7 cut(s) 1842, 1986, 2362, 3275, 4126, 4897, 5532
HpySE526I ACGT 7 cut(s) 1842, 1986, 2362, 3275, 4126, 4897, 5532
HspAI GCGC 4 cut(s) 40, 2860, 4623, 6078
Kpn2I TCCGGA 3 cut(s) 2623, 5263, 6718
Ksp22I TGATCA 1 cut(s) 192
KspAI GTTAAC 1 cut(s) 1779
LguI GCTCTTC 3 cut(s) 375, 2721, 3060
MabI ACCWGGT 1 cut(s) 776
MaeII ACGT 7 cut(s) 1842, 1986, 2362, 3275, 4126, 4897, 5532
MbiI CCGCTC 2 cut(s) 82, 1911
MfeI CAATTG 6 cut(s) 2895, 3300, 3547, 4072, 5199, 6654
MflI RGATCY 7 cut(s) 315, 562, 3469, 4154, 4397, 4908, 5834
Mph1103I ATGCAT 4 cut(s) 518, 1686, 2085, 6924
MroI TCCGGA 3 cut(s) 2623, 5263, 6718
MroXI GAANNNNTTC 4 cut(s) 402, 4488, 5925, 6663
MspA1I CMGCKG 2 cut(s) 683, 6972
MspI CCGG 8 cut(s) 545, 566, 1466, 1830, 2624, 5054, 5264, 6719
MunI CAATTG 6 cut(s) 2895, 3300, 3547, 4072, 5199, 6654
Mva1269I GAATGC 6 cut(s) 518, 824, 2778, 3832, 5731, 6811
MvnI CGCG 1 cut(s) 5511
NciI CCSGG 1 cut(s) 1466
NdeI CATATG 5 cut(s) 712, 884, 2784, 5067, 6522
NheI GCTAGC 1 cut(s) 220
NlaIV GGNNCC 8 cut(s) 543, 1370, 4475, 4849, 5315, 5912, 6304, 6961
NmeAIII GCCGAG 3 cut(s) 751, 3177, 6917
NmuCI GTSAC 6 cut(s) 1406, 2509, 3528, 4886, 6341, 6892
NruI TCGCGA 1 cut(s) 5511
NsiI ATGCAT 4 cut(s) 518, 1686, 2085, 6924
NspI RCATGY 6 cut(s) 1545, 2104, 2563, 4305, 4816, 6271
NspV TTCGAA 2 cut(s) 1073, 2719
OliI CACNNNNGTG 3 cut(s) 1989, 4839, 6294
PagI TCATGA 3 cut(s) 4726, 5542, 6181
PceI AGGCCT 1 cut(s) 3288
PciSI GCTCTTC 3 cut(s) 375, 2721, 3060
PcsI WCGNNNNNNNCGW 1 cut(s) 4928
PctI GAATGC 6 cut(s) 518, 824, 2778, 3832, 5731, 6811
PdmI GAANNNNTTC 4 cut(s) 402, 4488, 5925, 6663
PflFI GACNNNGTC 2 cut(s) 778, 1844
PflMI CCANNNNNTGG 2 cut(s) 5533, 5562
Ple19I CGATCG 1 cut(s) 6454
PmaCI CACGTG 1 cut(s) 1987
PmlI CACGTG 1 cut(s) 1987
Ppu21I YACGTR 1 cut(s) 1987
PpuMI RGGWCCY 1 cut(s) 6960
PshBI ATTAAT 1 cut(s) 6615
PsiI TTATAA 3 cut(s) 1214, 1905, 3965
Psp124BI GAGCTC 3 cut(s) 343, 387, 1642
Psp5II RGGWCCY 1 cut(s) 6960
PspCI CACGTG 1 cut(s) 1987
PspEI GGTNACC 3 cut(s) 3528, 4561, 6016
PspFI CCCAGC 2 cut(s) 203, 696
PspN4I GGNNCC 8 cut(s) 543, 1370, 4475, 4849, 5315, 5912, 6304, 6961
PspOMI GGGCCC 2 cut(s) 4473, 5910
PspPI GGNCC 9 cut(s) 299, 499, 4473, 4474, 4496, 5910, 5911, 5933, 6960
PspPPI RGGWCCY 1 cut(s) 6960
PstI CTGCAG 2 cut(s) 3347, 5036
PstNI CAGNNNCTG 2 cut(s) 412, 5274
PsuI RGATCY 7 cut(s) 315, 562, 3469, 4154, 4397, 4908, 5834
PsyI GACNNNGTC 2 cut(s) 778, 1844
PvuI CGATCG 1 cut(s) 6454
PvuII CAGCTG 1 cut(s) 6972
RruI TCGCGA 1 cut(s) 5511
RsaI GTAC 7 cut(s) 1674, 3480, 3894, 4442, 4963, 5879, 6418
RsaNI GTAC 7 cut(s) 1673, 3479, 3893, 4441, 4962, 5878, 6417
SacI GAGCTC 3 cut(s) 343, 387, 1642
SalI GTCGAC 1 cut(s) 2090
SapI GCTCTTC 3 cut(s) 375, 2721, 3060
Sau96I GGNCC 9 cut(s) 299, 499, 4473, 4474, 4496, 5910, 5911, 5933, 6960
SexAI ACCWGGT 1 cut(s) 776
SfcI CTRYAG 8 cut(s) 1914, 3343, 4369, 5032, 5210, 5275, 5472, 5806
SfuI TTCGAA 2 cut(s) 1073, 2719
SinI GGWCC 4 cut(s) 499, 4496, 5933, 6960
SpeI ACTAGT 1 cut(s) 944
SseBI AGGCCT 1 cut(s) 3288
SspI AATATT 5 cut(s) 1247, 1527, 1855, 3231, 3636
SstI GAGCTC 3 cut(s) 343, 387, 1642
StuI AGGCCT 1 cut(s) 3288
StyI CCWWGG 1 cut(s) 1363
TaaI ACNGT 9 cut(s) 877, 1940, 3628, 3713, 4496, 4892, 5770, 5933, 6347
TaiI ACGT 7 cut(s) 1845, 1989, 2365, 3278, 4129, 4900, 5535
TatI WGTACW 4 cut(s) 3478, 3892, 4961, 6416
TauI GCSGC 2 cut(s) 264, 683
TscAI CASTG 7 cut(s) 382, 880, 3716, 4895, 5377, 6350, 6832
TseFI GTSAC 6 cut(s) 1406, 2509, 3528, 4886, 6341, 6892
Tsp45I GTSAC 6 cut(s) 1406, 2509, 3528, 4886, 6341, 6892
TspGWI ACGGA 3 cut(s) 364, 566, 4937
TspRI CASTG 7 cut(s) 382, 880, 3716, 4895, 5377, 6350, 6832
Tth111I GACNNNGTC 2 cut(s) 778, 1844
Van91I CCANNNNNTGG 2 cut(s) 5533, 5562
VneI GTGCAC 2 cut(s) 4979, 6434
VpaK11BI GGWCC 4 cut(s) 499, 4496, 5933, 6960
VspI ATTAAT 1 cut(s) 6615
XbaI TCTAGA 1 cut(s) 5023
XceI RCATGY 6 cut(s) 1545, 2104, 2563, 4305, 4816, 6271
XcmI CCANNNNNNNNNTGG 1 cut(s) 6904
XmiI GTMKAC 1 cut(s) 2091
XmnI GAANNNNTTC 4 cut(s) 402, 4488, 5925, 6663
Zsp2I ATGCAT 4 cut(s) 518, 1686, 2085, 6924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.