Rmu_ssc0000248.1_g000009

Potato inhibitor I family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000248.1
OG0015550
Physical Location & Seq
Forward (+)
43032 .. 46266
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 774 bp
atgtctgatccttaccctccttgtgatggcggagaacttggatgccccagtctaagatacaaatattcatggcccgagttagttggaaaacctgggaaattagctaaacaaataatagagaaggacaaccactttgttacagtcttgatcctaaaaccaggcgattcaataactggagaccgttgttgcaaccgtgtgtatttttgggtggatgaccatggcaatgttacaagctactatccacccagaatagggcttcagaatcagaagcataagcttgagaagaaggtctgtgcaaattattggaaggctcaacgattaaactcagctgtagagagtaagaaggctctacatattaataaagtatataatcaatacgtgttgagagaatgtacagagaagaagggacgcttacagaagaaaatcgacaagcttataaatttcagcttttgcattgagaatcagattactttactggaaggacatgctaatattgggaaaacacctgtttatgatgactcaccaacaaaagaatctgcaacgaaatctaaggacatggaaatgacaattatcctccaaagcaatgactttatccttcatcctcttggtactctgttcaggaatttcaggcatttaatggattattttgatgattgtcaactcaaacatattcacagggaagggaatatggtggtggctgaattgcttgctaaggagagtatcaaaatcactctaatggtctatgcattctttacagacctcctgctttggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
potato_inhibit

Protein Analysis

257

Amino Acids

29.72

Weight (kDa)

8.76

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
potato_inhibit PF00280 21 - 85 1.1e-18 Potato inhibitor I family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 437
AciI CCGC 1 cut(s) 30
AclWI GGATC 2 cut(s) 2, 142
AcsI RAATTY 2 cut(s) 439, 622
AcuI CTGAAG 1 cut(s) 242
AfaI GTAC 2 cut(s) 394, 610
AfiI CCNNNNNNNGG 3 cut(s) 26, 251, 252
AflIII ACRYGT 1 cut(s) 378
AgsI TTSAA 1 cut(s) 168
AjnI CCWGG 2 cut(s) 91, 157
AluBI AGCT 6 cut(s) 104, 234, 277, 329, 433, 447
AluI AGCT 6 cut(s) 104, 234, 277, 329, 433, 447
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 2 cut(s) 2, 142
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 1 cut(s) 71
ApoI RAATTY 2 cut(s) 439, 622
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
AseI ATTAAT 1 cut(s) 357
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 1 cut(s) 513
AvaI CYCGRG 1 cut(s) 74
BccI CCATC 1 cut(s) 20
BciT130I CCWGG 2 cut(s) 93, 159
BcoDI GTCTC 1 cut(s) 171
BfmI CTRYAG 1 cut(s) 330
Bme1390I CCNGG 2 cut(s) 93, 159
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 72
BmrFI CCNGG 2 cut(s) 93, 159
BmrI ACTGGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 32
BmuI ACTGGG 1 cut(s) 42
BpmI CTGGAG 1 cut(s) 195
Bpu10I CCTNAGC 1 cut(s) 711
BpuEI CTTGAG 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 379
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 2 cut(s) 92, 217
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 251, 252
Bse1I ACTGG 3 cut(s) 48, 178, 480
Bse3DI GCAATG 2 cut(s) 229, 589
BseBI CCWGG 2 cut(s) 93, 159
BseDI CCNNGG 2 cut(s) 92, 217
BseGI GGATG 3 cut(s) 47, 217, 598
BseLI CCNNNNNNNGG 3 cut(s) 26, 251, 252
BseMI GCAATG 2 cut(s) 229, 589
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 3 cut(s) 48, 178, 480
BshFI GGCC 1 cut(s) 73
BsiHKCI CYCGRG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 420
BslI CCNNNNNNNGG 3 cut(s) 26, 251, 252
BsmAI GTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 420
BsmI GAATGC 1 cut(s) 746
BsnI GGCC 1 cut(s) 73
Bso31I GGTCTC 1 cut(s) 171
BsoBI CYCGRG 1 cut(s) 74
Bsp1407I TGTACA 1 cut(s) 392
Bsp143I GATC 2 cut(s) 7, 147
Bsp19I CCATGG 1 cut(s) 217
BspACI CCGC 1 cut(s) 30
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 1 cut(s) 338
BspPI GGATC 2 cut(s) 2, 142
BspTNI GGTCTC 1 cut(s) 171
BsrDI GCAATG 2 cut(s) 229, 589
BsrGI TGTACA 1 cut(s) 392
BsrI ACTGG 3 cut(s) 48, 178, 480
BssECI CCNNGG 2 cut(s) 92, 217
BssMI GATC 2 cut(s) 7, 147
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 2 cut(s) 93, 159
Bst4CI ACNGT 3 cut(s) 142, 182, 194
BstAUI TGTACA 1 cut(s) 392
BstBAI YACGTR 1 cut(s) 379
BstC8I GCNNGC 1 cut(s) 708
BstDEI CTNAG 4 cut(s) 53, 325, 549, 711
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 3 cut(s) 47, 217, 598
BstKTI GATC 2 cut(s) 10, 150
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 2 cut(s) 7, 147
BstNI CCWGG 2 cut(s) 93, 159
BstNSI RCATGY 1 cut(s) 488
BstSCI CCNGG 2 cut(s) 91, 157
BstSFI CTRYAG 1 cut(s) 330
BsuRI GGCC 1 cut(s) 73
BtgI CCRYGG 1 cut(s) 217
BtsCI GGATG 3 cut(s) 47, 217, 598
Cac8I GCNNGC 1 cut(s) 708
Cfr13I GGNCC 1 cut(s) 72
CseI GACGC 1 cut(s) 417
Csp6I GTAC 2 cut(s) 393, 609
CviAII CATG 4 cut(s) 69, 218, 485, 556
CviQI GTAC 2 cut(s) 393, 609
DdeI CTNAG 4 cut(s) 53, 325, 549, 711
DpnI GATC 2 cut(s) 9, 149
DpnII GATC 2 cut(s) 7, 147
EciI GGCGGA 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 217
Eco31I GGTCTC 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 242
Eco88I CYCGRG 1 cut(s) 74
EcoRII CCWGG 2 cut(s) 91, 157
EcoT14I CCWWGG 1 cut(s) 217
EcoT22I ATGCAT 1 cut(s) 748
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 4 cut(s) 72, 221, 488, 559
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 1 cut(s) 420
FatI CATG 4 cut(s) 68, 217, 484, 555
FokI GGATG 3 cut(s) 54, 224, 585
GsuI CTGGAG 1 cut(s) 195
HaeIII GGCC 1 cut(s) 73
HgaI GACGC 1 cut(s) 417
Hin1II CATG 4 cut(s) 72, 221, 488, 559
HincII GTYRAC 1 cut(s) 659
HindII GTYRAC 1 cut(s) 659
HindIII AAGCTT 2 cut(s) 275, 431
HinfI GANTC 5 cut(s) 164, 262, 460, 518, 533
HphI GGTGA 1 cut(s) 513
Hpy166II GTNNAC 1 cut(s) 659
Hpy188I TCNGA 4 cut(s) 7, 261, 267, 465
Hpy188III TCNNGA 2 cut(s) 145, 619
Hpy8I GTNNAC 1 cut(s) 659
HpyAV CCTTC 8 cut(s) 115, 280, 301, 337, 397, 473, 605, 674
HpyCH4III ACNGT 3 cut(s) 142, 182, 194
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 5 cut(s) 189, 296, 453, 539, 746
HpyF3I CTNAG 4 cut(s) 53, 325, 549, 711
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 4 cut(s) 72, 221, 488, 559
Kzo9I GATC 2 cut(s) 7, 147
LweI GCATC 1 cut(s) 32
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 2 cut(s) 136, 226
MalI GATC 2 cut(s) 9, 149
MboI GATC 2 cut(s) 7, 147
MboII GAAGA 3 cut(s) 295, 412, 430
MluCI AATT 6 cut(s) 98, 298, 439, 567, 622, 701
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 4 cut(s) 27, 584, 612, 770
Mph1103I ATGCAT 1 cut(s) 748
MseI TTAA 3 cut(s) 320, 357, 635
MslI CAYNNNNRTG 3 cut(s) 222, 560, 734
MspA1I CMGCKG 1 cut(s) 329
MspR9I CCNGG 2 cut(s) 93, 159
Mva1269I GAATGC 1 cut(s) 746
MvaI CCWGG 2 cut(s) 93, 159
NcoI CCATGG 1 cut(s) 217
NdeII GATC 2 cut(s) 7, 147
NlaIII CATG 4 cut(s) 72, 221, 488, 559
NsiI ATGCAT 1 cut(s) 748
NspI RCATGY 1 cut(s) 488
PctI GAATGC 1 cut(s) 746
PfeI GAWTC 4 cut(s) 164, 262, 460, 533
PleI GAGTC 1 cut(s) 512
PpsI GAGTC 1 cut(s) 512
Ppu21I YACGTR 1 cut(s) 379
PshBI ATTAAT 1 cut(s) 357
PsiI TTATAA 1 cut(s) 437
Psp6I CCWGG 2 cut(s) 91, 157
PspGI CCWGG 2 cut(s) 91, 157
PspPI GGNCC 1 cut(s) 72
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 2 cut(s) 394, 610
RsaNI GTAC 2 cut(s) 393, 609
RseI CAYNNNNRTG 3 cut(s) 222, 560, 734
SaqAI TTAA 3 cut(s) 320, 357, 635
Sau3AI GATC 2 cut(s) 7, 147
Sau96I GGNCC 1 cut(s) 72
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 2 cut(s) 93, 159
SfaNI GCATC 1 cut(s) 32
SfcI CTRYAG 1 cut(s) 330
SmiMI CAYNNNNRTG 3 cut(s) 222, 560, 734
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
Sse9I AATT 6 cut(s) 98, 298, 439, 567, 622, 701
SsiI CCGC 1 cut(s) 30
SspI AATATT 2 cut(s) 65, 493
StyD4I CCNGG 2 cut(s) 91, 157
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 3 cut(s) 142, 182, 194
TaiI ACGT 1 cut(s) 381
TaqI TCGA 1 cut(s) 426
TasI AATT 6 cut(s) 98, 298, 439, 567, 622, 701
TatI WGTACW 1 cut(s) 392
TfiI GAWTC 4 cut(s) 164, 262, 460, 533
Tru1I TTAA 3 cut(s) 320, 357, 635
Tru9I TTAA 3 cut(s) 320, 357, 635
TspDTI ATGAA 2 cut(s) 57, 587
VspI ATTAAT 1 cut(s) 357
XapI RAATTY 2 cut(s) 439, 622
XceI RCATGY 1 cut(s) 488
Zsp2I ATGCAT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.