Rmu_ssc0000250.1_g000018

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000250.1
OG0009016
Physical Location & Seq
Reverse (-)
92007 .. 95491
3485 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 447 bp
atggcaagtgtcgccggccggcctctcattcatgagtgggtaaatggtggtaattgccgatttccgatggggtttggccctgatggaacttttgatggtggtttcttcaatgctattatgagcttccctaacgattaccctaataaccctccgacagttagttttacctcagagatatggcaccctaatgtttactcggatggaaaggtttgcatttcaattcttcatcccccgggtgacgatcctaatggctacgagctggcgagtgagcgctggaatccagtccatacagttgagagcattgtgttgagtataatatcaatgctttcaagcccaaatgatgagtctcctgcaaatgttgaagcagcgaaacaatggagagaagatagatacggtttcaggaagagagtaggtcgctgcgtgaggaagtcacaagaaatgctataa

Protein Analysis

148

Amino Acids

16.52

Weight (kDa)

5.5

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 26 - 140 1.1e-37 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 180
AclWI GGATC 1 cut(s) 236
AcoI YGGCCR 1 cut(s) 16
AfeI AGCGCT 1 cut(s) 272
AgsI TTSAA 4 cut(s) 109, 219, 330, 362
AluBI AGCT 2 cut(s) 123, 259
AluI AGCT 2 cut(s) 123, 259
Alw26I GTCTC 1 cut(s) 351
AlwI GGATC 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 232
Aor51HI AGCGCT 1 cut(s) 272
AoxI GGCC 3 cut(s) 16, 20, 76
ApeKI GCWGC 2 cut(s) 365, 417
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AspLEI GCGC 1 cut(s) 273
AspS9I GGNCC 1 cut(s) 77
AsuC2I CCSGG 2 cut(s) 233, 234
AsuHPI GGTGA 1 cut(s) 248
AvaI CYCGRG 1 cut(s) 232
BanI GGYRCC 1 cut(s) 180
BbvI GCAGC 2 cut(s) 377, 404
BccI CCATC 4 cut(s) 61, 77, 89, 194
BcnI CCSGG 2 cut(s) 233, 234
BcoDI GTCTC 1 cut(s) 351
BfoI RGCGCY 1 cut(s) 274
BisI GCNGC 2 cut(s) 366, 418
BlsI GCNGC 2 cut(s) 367, 419
Bme1390I CCNGG 2 cut(s) 233, 234
BmeT110I CYCGRG 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 1 cut(s) 182
BmrFI CCNGG 2 cut(s) 233, 234
BpuMI CCSGG 2 cut(s) 233, 234
BsaJI CCNNGG 2 cut(s) 231, 232
Bse118I RCCGGY 2 cut(s) 14, 18
Bse1I ACTGG 1 cut(s) 281
BseDI CCNNGG 2 cut(s) 231, 232
BseGI GGATG 2 cut(s) 205, 226
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 281
BseX3I CGGCCG 1 cut(s) 16
BseXI GCAGC 2 cut(s) 377, 404
Bsh1285I CGRYCG 1 cut(s) 19
BshFI GGCC 3 cut(s) 18, 22, 78
BshNI GGYRCC 1 cut(s) 180
BsiEI CGRYCG 1 cut(s) 19
BsiHKCI CYCGRG 1 cut(s) 232
BsiSI CCGG 3 cut(s) 15, 19, 233
BsmAI GTCTC 1 cut(s) 351
BsnI GGCC 3 cut(s) 18, 22, 78
BsoBI CYCGRG 1 cut(s) 232
Bsp143I GATC 1 cut(s) 241
BspANI GGCC 3 cut(s) 18, 22, 78
BspCNI CTCAG 1 cut(s) 182
BspHI TCATGA 1 cut(s) 31
BspLI GGNNCC 1 cut(s) 182
BspPI GGATC 1 cut(s) 236
BspT107I GGYRCC 1 cut(s) 180
BsrFI RCCGGY 2 cut(s) 14, 18
BsrI ACTGG 1 cut(s) 281
BssAI RCCGGY 2 cut(s) 14, 18
BssECI CCNNGG 2 cut(s) 231, 232
BssMI GATC 1 cut(s) 241
Bst4CI ACNGT 3 cut(s) 157, 292, 395
Bst6I CTCTTC 1 cut(s) 398
BstC8I GCNNGC 3 cut(s) 16, 20, 261
BstDEI CTNAG 1 cut(s) 169
BstF5I GGATG 2 cut(s) 205, 226
BstH2I RGCGCY 1 cut(s) 274
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 1 cut(s) 244
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 1 cut(s) 241
BstMCI CGRYCG 1 cut(s) 19
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstSCI CCNGG 2 cut(s) 231, 232
BstV1I GCAGC 2 cut(s) 377, 404
BstZI CGGCCG 1 cut(s) 16
BsuRI GGCC 3 cut(s) 18, 22, 78
BtsCI GGATG 2 cut(s) 205, 226
Cac8I GCNNGC 3 cut(s) 16, 20, 261
CciI TCATGA 1 cut(s) 31
CfoI GCGC 1 cut(s) 273
Cfr10I RCCGGY 2 cut(s) 14, 18
Cfr13I GGNCC 1 cut(s) 77
Cfr9I CCCGGG 1 cut(s) 232
CviAII CATG 1 cut(s) 32
CviJI RGCY 7 cut(s) 18, 22, 78, 123, 252, 259, 333
CviKI_1 RGCY 7 cut(s) 18, 22, 78, 123, 252, 259, 333
DdeI CTNAG 1 cut(s) 169
DpnI GATC 1 cut(s) 243
DpnII GATC 1 cut(s) 241
EaeI YGGCCR 1 cut(s) 16
EagI CGGCCG 1 cut(s) 16
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
EclXI CGGCCG 1 cut(s) 16
Eco47III AGCGCT 1 cut(s) 272
Eco52I CGGCCG 1 cut(s) 16
Eco88I CYCGRG 1 cut(s) 232
FaeI CATG 1 cut(s) 35
FaiI YATR 6 cut(s) 33, 119, 178, 288, 314, 445
FatI CATG 1 cut(s) 31
Fnu4HI GCNGC 2 cut(s) 366, 418
FokI GGATG 2 cut(s) 212, 213
FseI GGCCGGCC 1 cut(s) 22
Fsp4HI GCNGC 2 cut(s) 366, 418
GlaI GCGC 1 cut(s) 272
GluI GCNGC 2 cut(s) 366, 418
HaeII RGCGCY 1 cut(s) 274
HaeIII GGCC 3 cut(s) 18, 22, 78
HapII CCGG 3 cut(s) 15, 19, 233
HhaI GCGC 1 cut(s) 273
Hin1II CATG 1 cut(s) 35
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HinfI GANTC 2 cut(s) 277, 344
HpaII CCGG 3 cut(s) 15, 19, 233
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 4 cut(s) 66, 153, 172, 199
Hpy188III TCNNGA 2 cut(s) 32, 400
Hpy8I GTNNAC 1 cut(s) 193
HpyCH4III ACNGT 3 cut(s) 157, 292, 395
HpyCH4V TGCA 2 cut(s) 213, 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 169
Hsp92II CATG 1 cut(s) 35
HspAI GCGC 1 cut(s) 271
KroI GCCGGC 2 cut(s) 14, 18
KroNI GCCGGC 2 cut(s) 16, 20
Kzo9I GATC 1 cut(s) 241
LpnPI CCDG 9 cut(s) 28, 32, 93, 245, 246, 259, 294, 363, 385
Lsp1109I GCAGC 2 cut(s) 377, 404
MaeIII GTNAC 2 cut(s) 236, 429
MalI GATC 1 cut(s) 243
MboI GATC 1 cut(s) 241
MboII GAAGA 4 cut(s) 97, 215, 395, 415
MluCI AATT 2 cut(s) 52, 219
MlyI GAGTC 1 cut(s) 353
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 4 cut(s) 33, 159, 178, 417
MroNI GCCGGC 2 cut(s) 14, 18
MslI CAYNNNNRTG 1 cut(s) 186
MspI CCGG 3 cut(s) 15, 19, 233
MspR9I CCNGG 2 cut(s) 233, 234
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 2 cut(s) 16, 20
NciI CCSGG 2 cut(s) 233, 234
NdeII GATC 1 cut(s) 241
NgoMIV GCCGGC 2 cut(s) 14, 18
NlaIII CATG 1 cut(s) 35
NlaIV GGNNCC 1 cut(s) 182
NmuCI GTSAC 2 cut(s) 236, 429
PagI TCATGA 1 cut(s) 31
PdiI GCCGGC 2 cut(s) 16, 20
PfeI GAWTC 1 cut(s) 277
PkrI GCNGC 2 cut(s) 367, 419
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
PspN4I GGNNCC 1 cut(s) 182
PspPI GGNCC 1 cut(s) 77
RigI GGCCGGCC 1 cut(s) 22
RseI CAYNNNNRTG 1 cut(s) 186
SatI GCNGC 2 cut(s) 366, 418
Sau3AI GATC 1 cut(s) 241
Sau96I GGNCC 1 cut(s) 77
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 2 cut(s) 233, 234
SetI ASST 5 cut(s) 125, 170, 210, 261, 415
SmaI CCCGGG 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 186
Sse9I AATT 2 cut(s) 52, 219
StyD4I CCNGG 2 cut(s) 231, 232
TaaI ACNGT 3 cut(s) 157, 292, 395
TasI AATT 2 cut(s) 52, 219
TfiI GAWTC 1 cut(s) 277
TseFI GTSAC 2 cut(s) 236, 429
TseI GCWGC 2 cut(s) 365, 417
Tsp45I GTSAC 2 cut(s) 236, 429
TspDTI ATGAA 2 cut(s) 20, 215
TspMI CCCGGG 1 cut(s) 232
XmaI CCCGGG 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.