Rmu_ssc0000281.1_g000018

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000281.1
OG0008613
Physical Location & Seq
Reverse (-)
69038 .. 70044
1007 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 729 bp
atgattaagaagcttgggagtcaatgtgaaatggatggtgtgcagtacttgaagcaccaaatgaaattggaggttggttgctctgaggagttgtttataagtgtggtgaagtcttataggcgagctgggttggctgagcaggcattgaagatgttctatgagcctccttggctgtttactgccctccatacattagcattaatgtttttggcgaaaacttttgtgaggggatgtaggcctaacattcacacctttacttccttgatgaagggttattttatggaaggcagagttagtgaagctctggacttgtggaaccgcatggttcgtgagggatttgggcccaatgttgttacatatacaactattatacatggtctctgctttgttaggaatatgcacgaagctgtatctgttttacatgagatggagagaaaaggctgctctccaaatgtgagcacatatagcacgtcactcttattgatgggtttgcaaaagctggcaaccttgttggtgctgttaaaaccatatacatgctcacaacatatgcacacaatcataaaagggatcaaggcttaccttcagcaatcactgacatggattccatcttatgctgcaaccacgaacactgaatatggccttctgttacttaccaacttgcttctcaatggacagaacaatatgcaaaacgtcggtagtaatcagggaccaattcatctgtatatatag

Protein Analysis

242

Amino Acids

27.43

Weight (kDa)

9.1

Isoelectric Point (pI)

29.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 76 - 109 1.7e-08 PPR repeat
PPR_2 PF13041 80 - 128 2.6e-17 PPR repeat family
PPR PF01535 84 - 113 1.1e-07 PPR repeat
PPR_3 PF13812 106 - 157 5.9e-14 Pentatricopeptide repeat domain
PPR_1 PF12854 112 - 144 4.3e-11 PPR repeat
PPR_2 PF13041 115 - 157 3e-16 PPR repeat family
PPR PF01535 118 - 148 4.9e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 98
AciI CCGC 1 cut(s) 319
AclWI GGATC 1 cut(s) 575
AcuI CTGAAG 1 cut(s) 566
AfaI GTAC 1 cut(s) 47
AgsI TTSAA 2 cut(s) 52, 148
AjiI CACGTC 1 cut(s) 471
AluBI AGCT 5 cut(s) 13, 125, 302, 407, 499
AluI AGCT 5 cut(s) 13, 125, 302, 407, 499
Alw21I GWGCWC 1 cut(s) 461
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 1 cut(s) 575
AoxI GGCC 3 cut(s) 236, 341, 637
ApaI GGGCCC 1 cut(s) 345
ApeKI GCWGC 2 cut(s) 441, 614
AseI ATTAAT 1 cut(s) 200
Asp700I GAANNNNTTC 1 cut(s) 152
AspS9I GGNCC 3 cut(s) 341, 342, 707
AsuHPI GGTGA 1 cut(s) 118
AvaII GGWCC 1 cut(s) 707
BaeGI GKGCMC 1 cut(s) 345
BanII GRGCYC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 461
BbvI GCAGC 2 cut(s) 428, 601
BccI CCATC 4 cut(s) 29, 421, 478, 613
BcoDI GTCTC 1 cut(s) 383
BglI GCCNNNNNGGC 1 cut(s) 169
BisI GCNGC 2 cut(s) 442, 615
BlpI GCTNAGC 1 cut(s) 135
BlsI GCNGC 2 cut(s) 443, 616
BmcAI AGTACT 1 cut(s) 47
Bme18I GGWCC 1 cut(s) 707
BmgBI CACGTC 1 cut(s) 471
BmgT120I GGNCC 3 cut(s) 341, 342, 707
BmiI GGNNCC 3 cut(s) 317, 343, 708
Bpu1102I GCTNAGC 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 167
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
BseDI CCNNGG 1 cut(s) 167
BseGI GGATG 2 cut(s) 40, 236
BseMII CTCAG 2 cut(s) 75, 126
BseRI GAGGAG 1 cut(s) 101
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 2 cut(s) 428, 601
BseYI CCCAGC 1 cut(s) 125
BsgI GTGCAG 1 cut(s) 62
BshFI GGCC 3 cut(s) 238, 343, 639
BsiHKAI GWGCWC 1 cut(s) 461
BslFI GGGAC 1 cut(s) 720
BsmAI GTCTC 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 720
BsnI GGCC 3 cut(s) 238, 343, 639
Bso31I GGTCTC 1 cut(s) 383
Bsp120I GGGCCC 1 cut(s) 341
Bsp1286I GDGCHC 2 cut(s) 345, 461
Bsp143I GATC 1 cut(s) 567
Bsp1720I GCTNAGC 1 cut(s) 135
BspACI CCGC 1 cut(s) 319
BspANI GGCC 3 cut(s) 238, 343, 639
BspCNI CTCAG 2 cut(s) 76, 127
BspLI GGNNCC 3 cut(s) 317, 343, 708
BspPI GGATC 1 cut(s) 575
BspTNI GGTCTC 1 cut(s) 383
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 1 cut(s) 567
BssT1I CCWWGG 1 cut(s) 167
BstC8I GCNNGC 3 cut(s) 123, 141, 501
BstDEI CTNAG 2 cut(s) 84, 135
BstF5I GGATG 2 cut(s) 40, 236
BstKTI GATC 1 cut(s) 570
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 1 cut(s) 567
BstMWI GCNNNNNNNGC 4 cut(s) 131, 140, 169, 465
BstNSI RCATGY 1 cut(s) 537
BstSLI GKGCMC 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 428, 601
BsuRI GGCC 3 cut(s) 238, 343, 639
BtrI CACGTC 1 cut(s) 471
BtsCI GGATG 2 cut(s) 40, 236
BtsIMutI CAGTG 2 cut(s) 590, 627
Cac8I GCNNGC 3 cut(s) 123, 141, 501
Cfr13I GGNCC 3 cut(s) 341, 342, 707
Csp6I GTAC 1 cut(s) 46
CviAII CATG 5 cut(s) 322, 374, 422, 534, 597
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 2 cut(s) 84, 135
DpnI GATC 1 cut(s) 569
DpnII GATC 1 cut(s) 567
Eco130I CCWWGG 1 cut(s) 167
Eco147I AGGCCT 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 707
Eco57I CTGAAG 1 cut(s) 566
EcoT14I CCWWGG 1 cut(s) 167
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 167
FaeI CATG 5 cut(s) 325, 377, 425, 537, 600
FaqI GGGAC 1 cut(s) 720
FatI CATG 5 cut(s) 321, 373, 421, 533, 596
FauNDI CATATG 1 cut(s) 546
Fnu4HI GCNGC 2 cut(s) 442, 615
FokI GGATG 2 cut(s) 47, 243
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 2 cut(s) 442, 615
GluI GCNGC 2 cut(s) 442, 615
GsaI CCCAGC 1 cut(s) 129
HaeIII GGCC 3 cut(s) 238, 343, 639
Hin1II CATG 5 cut(s) 325, 377, 425, 537, 600
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 2 cut(s) 19, 601
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 1 cut(s) 85
Hpy188III TCNNGA 2 cut(s) 305, 329
Hpy8I GTNNAC 1 cut(s) 177
Hpy99I CGWCG 1 cut(s) 695
HpyAV CCTTC 4 cut(s) 262, 278, 590, 650
HpyCH4IV ACGT 2 cut(s) 470, 690
HpyCH4V TGCA 6 cut(s) 43, 400, 493, 550, 617, 685
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 140, 169, 465
HpyF3I CTNAG 2 cut(s) 84, 135
HpySE526I ACGT 2 cut(s) 470, 690
Hsp92II CATG 5 cut(s) 325, 377, 425, 537, 600
Kzo9I GATC 1 cut(s) 567
LpnPI CCDG 5 cut(s) 111, 125, 290, 485, 689
Lsp1109I GCAGC 2 cut(s) 428, 601
MaeII ACGT 2 cut(s) 470, 690
MaeIII GTNAC 3 cut(s) 352, 471, 645
MalI GATC 1 cut(s) 569
MboI GATC 1 cut(s) 567
MboII GAAGA 1 cut(s) 160
MhlI GDGCHC 2 cut(s) 345, 461
MluCI AATT 2 cut(s) 65, 711
MlyI GAGTC 1 cut(s) 28
MnlI CCTC 6 cut(s) 64, 79, 174, 194, 219, 325
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 3 cut(s) 6, 200, 521
MslI CAYNNNNRTG 2 cut(s) 532, 595
MwoI GCNNNNNNNGC 4 cut(s) 131, 140, 169, 465
NdeI CATATG 1 cut(s) 546
NdeII GATC 1 cut(s) 567
NlaIII CATG 5 cut(s) 325, 377, 425, 537, 600
NlaIV GGNNCC 3 cut(s) 317, 343, 708
NmuCI GTSAC 1 cut(s) 471
NspI RCATGY 1 cut(s) 537
PceI AGGCCT 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 1 cut(s) 601
PkrI GCNGC 2 cut(s) 443, 616
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
PshBI ATTAAT 1 cut(s) 200
PsiI TTATAA 1 cut(s) 98
PspFI CCCAGC 1 cut(s) 125
PspN4I GGNNCC 3 cut(s) 317, 343, 708
PspOMI GGGCCC 1 cut(s) 341
PspPI GGNCC 3 cut(s) 341, 342, 707
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 2 cut(s) 532, 595
SaqAI TTAA 3 cut(s) 6, 200, 521
SatI GCNGC 2 cut(s) 442, 615
Sau3AI GATC 1 cut(s) 567
Sau96I GGNCC 3 cut(s) 341, 342, 707
ScaI AGTACT 1 cut(s) 47
SchI GAGTC 1 cut(s) 28
SduI GDGCHC 2 cut(s) 345, 461
SinI GGWCC 1 cut(s) 707
SmiMI CAYNNNNRTG 2 cut(s) 532, 595
Sse9I AATT 2 cut(s) 65, 711
SseBI AGGCCT 1 cut(s) 238
SsiI CCGC 1 cut(s) 319
StuI AGGCCT 1 cut(s) 238
StyI CCWWGG 1 cut(s) 167
TaiI ACGT 2 cut(s) 473, 693
TasI AATT 2 cut(s) 65, 711
TatI WGTACW 1 cut(s) 45
TfiI GAWTC 1 cut(s) 601
Tru1I TTAA 3 cut(s) 6, 200, 521
Tru9I TTAA 3 cut(s) 6, 200, 521
TscAI CASTG 2 cut(s) 597, 634
TseFI GTSAC 1 cut(s) 471
TseI GCWGC 2 cut(s) 441, 614
Tsp45I GTSAC 1 cut(s) 471
TspDTI ATGAA 3 cut(s) 77, 281, 704
TspRI CASTG 2 cut(s) 597, 634
VpaK11BI GGWCC 1 cut(s) 707
VspI ATTAAT 1 cut(s) 200
XceI RCATGY 1 cut(s) 537
XmnI GAANNNNTTC 1 cut(s) 152
ZrmI AGTACT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.