Rmu_ssc0000313.1_g000005

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000313.1
OG0008681
Physical Location & Seq
Reverse (-)
24569 .. 25710
1142 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 639 bp
atgttgacagctactgatgatgcagttcaactagtggttgatcggccatataaggcaggggaatctatttctgtttggtgtgggccacagcctaattcaaaattgcttataaattacggttttgttgacgaagataattcttatgaccgtttgatggttgaggcagctttgaatactgaggatccacaatatcaagataaaagaatggttgctcagagaaatggaaaattatcagtacaagcttttcaggtatatgtcgggaaggaaaaggaaactgtctcggatatgcttccctatctgcgactaggctatgtttcagatccttcggagatgcagtctgtcatctcttctcaaggtccaatttgtccagttagtccctgcatggaaagtgcagttttggaccaacttgctcaatattttagaagtcggctggctggctatccaactactctgattgaagatgagtctatgttggcggatcctaatttgaatccaaagaagcgagttgctacacagcttgttaggtcagaaaagaaaatactacatgcgtgcctgcaggtgactcttgattttataaatcagttacccgatctcagtgtaactccatgccctgccccttacgccccttcgttgagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind

Protein Analysis

212

Amino Acids

23.62

Weight (kDa)

4.87

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rubis-subs-bind PF09273 59 - 181 1.4e-32 Rubisco LSMT substrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 110, 575
AarI CACCTGC 1 cut(s) 547
Acc36I ACCTGC 1 cut(s) 547
AciI CCGC 1 cut(s) 476
AclWI GGATC 5 cut(s) 176, 189, 314, 473, 486
AcoI YGGCCR 1 cut(s) 44
AfaI GTAC 1 cut(s) 237
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 5 cut(s) 29, 99, 172, 458, 490
AhlI ACTAGT 1 cut(s) 31
AluBI AGCT 4 cut(s) 11, 167, 242, 517
AluI AGCT 4 cut(s) 11, 167, 242, 517
Alw26I GTCTC 1 cut(s) 283
AlwI GGATC 5 cut(s) 176, 189, 314, 473, 486
AlwNI CAGNNNCTG 1 cut(s) 14
AoxI GGCC 2 cut(s) 44, 83
ApeKI GCWGC 1 cut(s) 164
AspS9I GGNCC 3 cut(s) 83, 356, 400
AsuHPI GGTGA 1 cut(s) 571
AvaII GGWCC 2 cut(s) 356, 400
BamHI GGATCC 2 cut(s) 181, 478
BbvI GCAGC 1 cut(s) 176
BccI CCATC 1 cut(s) 148
BcoDI GTCTC 1 cut(s) 283
BcuI ACTAGT 1 cut(s) 31
BfaI CTAG 2 cut(s) 32, 305
BfmI CTRYAG 1 cut(s) 554
BfuAI ACCTGC 1 cut(s) 547
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
Bme18I GGWCC 2 cut(s) 356, 400
BmgT120I GGNCC 3 cut(s) 83, 356, 400
BmiI GGNNCC 2 cut(s) 183, 480
BmsI GCATC 2 cut(s) 10, 321
BpuEI CTTGAG 1 cut(s) 336
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 368
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMII CTCAG 3 cut(s) 168, 227, 607
BseNI ACTGG 1 cut(s) 368
BseXI GCAGC 1 cut(s) 176
BsgI GTGCAG 1 cut(s) 411
BshFI GGCC 2 cut(s) 46, 85
BslFI GGGAC 1 cut(s) 360
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 1 cut(s) 283
BsmFI GGGAC 1 cut(s) 360
BsnI GGCC 2 cut(s) 46, 85
Bsp143I GATC 5 cut(s) 40, 181, 319, 478, 589
BspACI CCGC 1 cut(s) 476
BspANI GGCC 2 cut(s) 46, 85
BspCNI CTCAG 3 cut(s) 169, 226, 606
BspLI GGNNCC 2 cut(s) 183, 480
BspMAI CTGCAG 1 cut(s) 558
BspMI ACCTGC 1 cut(s) 547
BspPI GGATC 5 cut(s) 176, 189, 314, 473, 486
BsrI ACTGG 1 cut(s) 368
BssMI GATC 5 cut(s) 40, 181, 319, 478, 589
Bst4CI ACNGT 3 cut(s) 119, 149, 277
Bst6I CTCTTC 1 cut(s) 352
BstC8I GCNNGC 4 cut(s) 432, 436, 550, 554
BstDEI CTNAG 3 cut(s) 177, 213, 593
BstKTI GATC 5 cut(s) 43, 184, 322, 481, 592
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 5 cut(s) 40, 181, 319, 478, 589
BstMWI GCNNNNNNNGC 1 cut(s) 620
BstNSI RCATGY 1 cut(s) 548
BstSFI CTRYAG 1 cut(s) 554
BstV1I GCAGC 1 cut(s) 176
BstX2I RGATCY 3 cut(s) 181, 319, 478
BstYI RGATCY 3 cut(s) 181, 319, 478
BsuRI GGCC 2 cut(s) 46, 85
BtsIMutI CAGTG 1 cut(s) 601
BveI ACCTGC 1 cut(s) 547
Cac8I GCNNGC 4 cut(s) 432, 436, 550, 554
CaiI CAGNNNCTG 1 cut(s) 14
Cfr13I GGNCC 3 cut(s) 83, 356, 400
Csp6I GTAC 1 cut(s) 236
CviAII CATG 3 cut(s) 382, 545, 606
CviQI GTAC 1 cut(s) 236
DdeI CTNAG 3 cut(s) 177, 213, 593
DpnI GATC 5 cut(s) 42, 183, 321, 480, 591
DpnII GATC 5 cut(s) 40, 181, 319, 478, 589
EaeI YGGCCR 1 cut(s) 44
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
EciI GGCGGA 1 cut(s) 491
Eco47I GGWCC 2 cut(s) 356, 400
FaeI CATG 3 cut(s) 385, 548, 609
FaqI GGGAC 1 cut(s) 360
FatI CATG 3 cut(s) 381, 544, 605
Fnu4HI GCNGC 1 cut(s) 165
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 2 cut(s) 32, 305
GluI GCNGC 1 cut(s) 165
HaeIII GGCC 2 cut(s) 46, 85
Hin1II CATG 3 cut(s) 385, 548, 609
HincII GTYRAC 2 cut(s) 6, 127
HindII GTYRAC 2 cut(s) 6, 127
HindIII AAGCTT 1 cut(s) 240
HinfI GANTC 4 cut(s) 62, 464, 490, 562
HphI GGTGA 1 cut(s) 571
Hpy166II GTNNAC 2 cut(s) 6, 127
Hpy188I TCNGA 6 cut(s) 216, 283, 319, 328, 453, 529
Hpy188III TCNNGA 3 cut(s) 194, 259, 566
Hpy8I GTNNAC 2 cut(s) 6, 127
HpyAV CCTTC 3 cut(s) 256, 333, 636
HpyCH4III ACNGT 3 cut(s) 119, 149, 277
HpyCH4V TGCA 5 cut(s) 23, 334, 381, 392, 556
HpyF10VI GCNNNNNNNGC 1 cut(s) 620
HpyF3I CTNAG 3 cut(s) 177, 213, 593
Hsp92II CATG 3 cut(s) 385, 548, 609
Kzo9I GATC 5 cut(s) 40, 181, 319, 478, 589
LpnPI CCDG 9 cut(s) 42, 233, 381, 391, 416, 420, 542, 566, 624
Lsp1109I GCAGC 1 cut(s) 176
LweI GCATC 2 cut(s) 10, 321
MaeI CTAG 2 cut(s) 32, 305
MaeIII GTNAC 3 cut(s) 559, 582, 598
MalI GATC 5 cut(s) 42, 183, 321, 480, 591
MboI GATC 5 cut(s) 40, 181, 319, 478, 589
MboII GAAGA 3 cut(s) 143, 339, 470
MflI RGATCY 3 cut(s) 181, 319, 478
MluCI AATT 7 cut(s) 94, 101, 112, 136, 227, 360, 484
MlyI GAGTC 2 cut(s) 473, 556
MmeI TCCRAC 1 cut(s) 467
MnlI CCTC 2 cut(s) 154, 172
MwoI GCNNNNNNNGC 1 cut(s) 620
NdeII GATC 5 cut(s) 40, 181, 319, 478, 589
NlaIII CATG 3 cut(s) 385, 548, 609
NlaIV GGNNCC 2 cut(s) 183, 480
NmuCI GTSAC 1 cut(s) 559
NspI RCATGY 1 cut(s) 548
PaqCI CACCTGC 1 cut(s) 547
PfeI GAWTC 2 cut(s) 62, 490
PkrI GCNGC 1 cut(s) 166
PleI GAGTC 2 cut(s) 472, 556
PpsI GAGTC 2 cut(s) 472, 556
PsiI TTATAA 2 cut(s) 110, 575
PspN4I GGNNCC 2 cut(s) 183, 480
PspPI GGNCC 3 cut(s) 83, 356, 400
PstI CTGCAG 1 cut(s) 558
PstNI CAGNNNCTG 1 cut(s) 14
PsuI RGATCY 3 cut(s) 181, 319, 478
RsaI GTAC 1 cut(s) 237
RsaNI GTAC 1 cut(s) 236
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 5 cut(s) 40, 181, 319, 478, 589
Sau96I GGNCC 3 cut(s) 83, 356, 400
SbfI CCTGCAGG 1 cut(s) 558
SchI GAGTC 2 cut(s) 473, 556
SdaI CCTGCAGG 1 cut(s) 558
SetI ASST 8 cut(s) 13, 169, 244, 252, 358, 519, 527, 561
SfaNI GCATC 2 cut(s) 10, 321
SfcI CTRYAG 1 cut(s) 554
SinI GGWCC 2 cut(s) 356, 400
SmlI CTYRAG 1 cut(s) 351
SmoI CTYRAG 1 cut(s) 351
SpeI ACTAGT 1 cut(s) 31
Sse8387I CCTGCAGG 1 cut(s) 558
Sse9I AATT 7 cut(s) 94, 101, 112, 136, 227, 360, 484
SsiI CCGC 1 cut(s) 476
SspI AATATT 1 cut(s) 416
SspMI CTAG 2 cut(s) 32, 305
TaaI ACNGT 3 cut(s) 119, 149, 277
TasI AATT 7 cut(s) 94, 101, 112, 136, 227, 360, 484
TatI WGTACW 1 cut(s) 235
TfiI GAWTC 2 cut(s) 62, 490
TscAI CASTG 1 cut(s) 601
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 1 cut(s) 164
Tsp45I GTSAC 1 cut(s) 559
TspRI CASTG 1 cut(s) 601
VpaK11BI GGWCC 2 cut(s) 356, 400
XceI RCATGY 1 cut(s) 548
XspI CTAG 2 cut(s) 32, 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.