Rmu_ssc0000322.1_g000013
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB NOP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000322.1
OG0015752
Physical Location & Seq
Forward (+)
50110 .. 60526
10417 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1377 bp
atggtatatgcaattgacataaatgaagggcggttgagaatcctcaaagaaatggctaagttgcaccaagtagatggtgtcatcaccactatccatgctgatatgcgtagttatactgacaataatactatgaagtttgataaagttctgctggatgctccatgttctggactgcgtgttctccccaagggcagacttgcgttggaacagtggagattagaggatatggaggagcttaagactttgcaggatgagctccttgattttgcttctcgtcttgatcctgaaaatgatccacttcgtctgaaagatgatgatgaagatgtgctagaggcagaagtaccttacttgagtataataggcgcattattgtacttagctcaatgcacaagaccgggcatctcatttgcagtgaacttgttagctaaggtacaatctatttatcgtgaagcaaatggtgttgcccatagattggcacatcttgctaggttatcttatttagatgattattggttagatgagactcctgtgattatccaggatgttctctatgaggattcttgtactagtactcgagaccaagagcagccttccttcatctcaccatctcactctcactctcctcaatctcactcgattcctcagccttctccgatggcaaggttcaaatcgaagtcgctattggtccggtggtttactctctctacgcgccgaagcttgaggctgccttcaaaactaggtcaagctcgggaattgattcatctacaggtgcgactccgcctctctggctctctccctcacaatcggtcttcctcacctctgcggctcttcctctcagctctcactcttgaccctcggcgtcagcaaatccaatctcctctccagaaccctcaacgtcgacgtagctggccaatcggtcatcaaggcccagatttaggacacttttggccaagaaaggtcagctttctgaatattaatggtttcggtttgaagagcagtaagaaacccaaatcaaagactccggcaaagaagcccaaagacagtgttcttgagcagactgagttcttcgctgagaacaaggacattgctggatttgccgtccccattggtcttttgcgacggcaaattggctttccgcgaccgcggttccttaaacaatattcgttagatattgtggagaagaacgacgacgaatctccggtgccgagtccacctcggccttctaaacctgatgctaatcctaagccagttgctgatgggaagacaaagtcgaataaagcaatacaaagccgcatgcagtcgcctgaaactgccgaaaacccgccggaaagcttgaagcttttaggctctgtttcttcgccgacggtcgtcgactga

Protein Analysis

458

Amino Acids

51.74

Weight (kDa)

9.27

Isoelectric Point (pI)

60.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltr_RsmB-F PF01189 2 - 90 4.6e-12 16S rRNA methyltransferase RsmB/F
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1201
AccB7I CCANNNNNTGG 2 cut(s) 167, 472
AccI GTMKAC 2 cut(s) 898, 1371
AccII CGCG 3 cut(s) 709, 1138, 1144
AciI CCGC 8 cut(s) 31, 778, 823, 1136, 1142, 1144, 1291, 1322
AclWI GGATC 2 cut(s) 275, 287
AcoI YGGCCR 2 cut(s) 908, 947
AcyI GRCGYC 1 cut(s) 859
AfaI GTAC 5 cut(s) 342, 374, 432, 565, 571
AfiI CCNNNNNNNGG 3 cut(s) 167, 472, 935
AflII CTTAAG 1 cut(s) 236
AgsI TTSAA 4 cut(s) 667, 732, 991, 1336
AhlI ACTAGT 1 cut(s) 566
AjnI CCWGG 1 cut(s) 537
AjuI GAANNNNNNNTTGG 2 cut(s) 665, 697
AloI GAACNNNNNNTCC 2 cut(s) 162, 194
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 2 cut(s) 515, 570
AlwI GGATC 2 cut(s) 275, 287
AlwNI CAGNNNCTG 1 cut(s) 1253
Ama87I CYCGRG 2 cut(s) 573, 747
AoxI GGCC 4 cut(s) 908, 925, 947, 1217
ApeKI GCWGC 2 cut(s) 586, 724
AseI ATTAAT 1 cut(s) 975
Asp700I GAANNNNTTC 1 cut(s) 756
AspLEI GCGC 2 cut(s) 365, 711
AspS9I GGNCC 2 cut(s) 685, 926
AsuC2I CCSGG 1 cut(s) 396
AsuHPI GGTGA 3 cut(s) 76, 594, 807
AvaI CYCGRG 2 cut(s) 573, 747
AvaII GGWCC 1 cut(s) 685
BalI TGGCCA 2 cut(s) 910, 949
BanI GGYRCC 1 cut(s) 1201
BanII GRGCYC 1 cut(s) 258
BbsI GAAGAC 2 cut(s) 801, 1268
Bbv12I GWGCWC 1 cut(s) 258
BbvCI CCTCAGC 1 cut(s) 642
BbvI GCAGC 2 cut(s) 598, 711
BccI CCATC 4 cut(s) 68, 613, 649, 1250
BceAI ACGGC 2 cut(s) 1082, 1136
BciT130I CCWGG 1 cut(s) 539
BcnI CCSGG 1 cut(s) 396
BcoDI GTCTC 2 cut(s) 515, 570
BcuI ACTAGT 1 cut(s) 566
BfaI CTAG 4 cut(s) 329, 486, 567, 737
BfmI CTRYAG 1 cut(s) 764
BfrI CTTAAG 1 cut(s) 236
BglI GCCNNNNNGGC 1 cut(s) 786
BisI GCNGC 4 cut(s) 587, 725, 824, 1291
BlsI GCNGC 4 cut(s) 588, 726, 825, 1292
BmcAI AGTACT 1 cut(s) 571
Bme1390I CCNGG 2 cut(s) 396, 539
Bme18I GGWCC 1 cut(s) 685
BmeT110I CYCGRG 2 cut(s) 573, 747
BmgT120I GGNCC 2 cut(s) 685, 926
BmiI GGNNCC 2 cut(s) 1148, 1203
BmrFI CCNGG 2 cut(s) 396, 539
BmsI GCATC 3 cut(s) 145, 408, 1222
BoxI GACNNNNGTC 1 cut(s) 1367
BpiI GAAGAC 2 cut(s) 801, 1268
BplI GAGNNNNNCTC 2 cut(s) 1198, 1230
BpmI CTGGAG 1 cut(s) 866
Bpu10I CCTNAGC 3 cut(s) 426, 642, 1242
BpuEI CTTGAG 3 cut(s) 370, 739, 1070
BpuMI CCSGG 1 cut(s) 396
BsaBI GATNNNNATC 1 cut(s) 1236
BsaHI GRCGYC 1 cut(s) 859
BsaI GGTCTC 1 cut(s) 570
BsaJI CCNNGG 4 cut(s) 186, 854, 1142, 1214
BsaWI WCCGGW 2 cut(s) 687, 1198
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 472, 935
Bse1I ACTGG 1 cut(s) 1247
Bse3DI GCAATG 1 cut(s) 1083
Bse8I GATNNNNATC 1 cut(s) 1236
BseBI CCWGG 1 cut(s) 539
BseDI CCNNGG 4 cut(s) 186, 854, 1142, 1214
BseGI GGATG 3 cut(s) 160, 256, 547
BseJI GATNNNNATC 1 cut(s) 1236
BseLI CCNNNNNNNGG 3 cut(s) 167, 472, 935
BseMI GCAATG 1 cut(s) 1083
BseMII CTCAG 4 cut(s) 656, 849, 1050, 1062
BseNI ACTGG 1 cut(s) 1247
BseRI GAGGAG 3 cut(s) 245, 612, 867
BseXI GCAGC 2 cut(s) 598, 711
Bsh1236I CGCG 3 cut(s) 709, 1138, 1144
Bsh1285I CGRYCG 2 cut(s) 1142, 1368
BshFI GGCC 4 cut(s) 910, 927, 949, 1219
BshNI GGYRCC 1 cut(s) 1201
BsiEI CGRYCG 2 cut(s) 1142, 1368
BsiHKAI GWGCWC 1 cut(s) 258
BsiHKCI CYCGRG 2 cut(s) 573, 747
BsiSI CCGG 5 cut(s) 395, 688, 1022, 1199, 1325
BslFI GGGAC 1 cut(s) 1085
BslI CCNNNNNNNGG 3 cut(s) 167, 472, 935
BsmAI GTCTC 2 cut(s) 515, 570
BsmFI GGGAC 1 cut(s) 1085
BsnI GGCC 4 cut(s) 910, 927, 949, 1219
Bso31I GGTCTC 1 cut(s) 570
BsoBI CYCGRG 2 cut(s) 573, 747
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 2 cut(s) 280, 292
BspACI CCGC 8 cut(s) 31, 778, 823, 1136, 1142, 1144, 1291, 1322
BspANI GGCC 4 cut(s) 910, 927, 949, 1219
BspCNI CTCAG 4 cut(s) 655, 848, 1051, 1063
BspFNI CGCG 3 cut(s) 709, 1138, 1144
BspLI GGNNCC 2 cut(s) 1148, 1203
BspPI GGATC 2 cut(s) 275, 287
BspQI GCTCTTC 2 cut(s) 833, 986
BspT107I GGYRCC 1 cut(s) 1201
BspTI CTTAAG 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 570
BsrDI GCAATG 1 cut(s) 1083
BsrI ACTGG 1 cut(s) 1247
BssECI CCNNGG 4 cut(s) 186, 854, 1142, 1214
BssMI GATC 2 cut(s) 280, 292
BssNI GRCGYC 1 cut(s) 859
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 539
Bst4CI ACNGT 3 cut(s) 210, 1043, 1366
Bst6I CTCTTC 2 cut(s) 833, 986
BstACI GRCGYC 1 cut(s) 859
BstAFI CTTAAG 1 cut(s) 236
BstAPI GCANNNNNTGC 1 cut(s) 482
BstC8I GCNNGC 2 cut(s) 908, 1295
BstDEI CTNAG 8 cut(s) 57, 376, 426, 642, 835, 1059, 1071, 1242
BstDSI CCRYGG 1 cut(s) 1142
BstF5I GGATG 3 cut(s) 160, 256, 547
BstFNI CGCG 3 cut(s) 709, 1138, 1144
BstHHI GCGC 2 cut(s) 365, 711
BstKTI GATC 2 cut(s) 283, 295
BstMAI GTCTC 2 cut(s) 515, 570
BstMBI GATC 2 cut(s) 280, 292
BstMCI CGRYCG 2 cut(s) 1142, 1368
BstMWI GCNNNNNNNGC 4 cut(s) 253, 482, 786, 1094
BstNI CCWGG 1 cut(s) 539
BstNSI RCATGY 1 cut(s) 1297
BstPAI GACNNNNGTC 1 cut(s) 1367
BstSCI CCNGG 2 cut(s) 394, 537
BstSFI CTRYAG 1 cut(s) 764
BstUI CGCG 3 cut(s) 709, 1138, 1144
BstV1I GCAGC 2 cut(s) 598, 711
BstV2I GAAGAC 2 cut(s) 801, 1268
BstXI CCANNNNNNTGG 1 cut(s) 74
BsuRI GGCC 4 cut(s) 910, 927, 949, 1219
BtgI CCRYGG 1 cut(s) 1142
BtsCI GGATG 3 cut(s) 160, 256, 547
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 3 cut(s) 215, 417, 1048
Cac8I GCNNGC 2 cut(s) 908, 1295
CaiI CAGNNNCTG 1 cut(s) 1253
CfoI GCGC 2 cut(s) 365, 711
Cfr13I GGNCC 2 cut(s) 685, 926
Cfr42I CCGCGG 1 cut(s) 1145
CseI GACGC 1 cut(s) 848
Csp6I GTAC 5 cut(s) 341, 373, 431, 564, 570
CviAII CATG 3 cut(s) 95, 162, 1294
CviQI GTAC 5 cut(s) 341, 373, 431, 564, 570
DdeI CTNAG 8 cut(s) 57, 376, 426, 642, 835, 1059, 1071, 1242
DpnI GATC 2 cut(s) 282, 294
DpnII GATC 2 cut(s) 280, 292
EaeI YGGCCR 2 cut(s) 908, 947
Eam1104I CTCTTC 2 cut(s) 833, 986
EarI CTCTTC 2 cut(s) 833, 986
EciI GGCGGA 1 cut(s) 767
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 258
Eco31I GGTCTC 1 cut(s) 570
Eco47I GGWCC 1 cut(s) 685
Eco53kI GAGCTC 1 cut(s) 256
Eco88I CYCGRG 2 cut(s) 573, 747
EcoICRI GAGCTC 1 cut(s) 256
EcoRII CCWGG 1 cut(s) 537
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 3 cut(s) 98, 165, 1297
FalI AAGNNNNNCTT 2 cut(s) 947, 979
FaqI GGGAC 1 cut(s) 1085
FatI CATG 3 cut(s) 94, 161, 1293
FauI CCCGC 1 cut(s) 1329
FblI GTMKAC 2 cut(s) 898, 1371
Fnu4HI GCNGC 4 cut(s) 587, 725, 824, 1291
FokI GGATG 3 cut(s) 167, 263, 554
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 4 cut(s) 587, 725, 824, 1291
FspBI CTAG 4 cut(s) 329, 486, 567, 737
GlaI GCGC 2 cut(s) 364, 710
GluI GCNGC 4 cut(s) 587, 725, 824, 1291
GsuI CTGGAG 1 cut(s) 866
HaeIII GGCC 4 cut(s) 910, 927, 949, 1219
HapII CCGG 5 cut(s) 395, 688, 1022, 1199, 1325
HgaI GACGC 1 cut(s) 848
HhaI GCGC 2 cut(s) 365, 711
Hin1I GRCGYC 1 cut(s) 859
Hin1II CATG 3 cut(s) 98, 165, 1297
Hin6I GCGC 2 cut(s) 363, 709
HinP1I GCGC 2 cut(s) 363, 709
HincII GTYRAC 2 cut(s) 899, 1372
HindII GTYRAC 2 cut(s) 899, 1372
HindIII AAGCTT 3 cut(s) 715, 1330, 1337
HinfI GANTC 9 cut(s) 39, 523, 557, 637, 757, 774, 1018, 1193, 1207
HpaII CCGG 5 cut(s) 395, 688, 1022, 1199, 1325
HphI GGTGA 3 cut(s) 76, 594, 807
Hpy166II GTNNAC 5 cut(s) 415, 696, 899, 1211, 1372
Hpy188I TCNGA 3 cut(s) 306, 654, 969
Hpy188III TCNNGA 9 cut(s) 168, 278, 284, 446, 575, 749, 848, 883, 1049
Hpy8I GTNNAC 5 cut(s) 415, 696, 899, 1211, 1372
Hpy99I CGWCG 7 cut(s) 900, 903, 1122, 1190, 1193, 1366, 1373
HpyAV CCTTC 6 cut(s) 20, 600, 604, 657, 738, 1230
HpyCH4III ACNGT 3 cut(s) 210, 1043, 1366
HpyCH4IV ACGT 2 cut(s) 895, 901
HpyCH4V TGCA 6 cut(s) 11, 64, 247, 387, 410, 1297
HpyF10VI GCNNNNNNNGC 4 cut(s) 253, 482, 786, 1094
HpyF3I CTNAG 8 cut(s) 57, 376, 426, 642, 835, 1059, 1071, 1242
HpySE526I ACGT 2 cut(s) 895, 901
Hsp92I GRCGYC 1 cut(s) 859
Hsp92II CATG 3 cut(s) 98, 165, 1297
HspAI GCGC 2 cut(s) 363, 709
KspI CCGCGG 1 cut(s) 1145
Kzo9I GATC 2 cut(s) 280, 292
LguI GCTCTTC 2 cut(s) 833, 986
LmnI GCTCC 3 cut(s) 163, 232, 261
Lsp1109I GCAGC 2 cut(s) 598, 711
LweI GCATC 3 cut(s) 145, 408, 1222
MaeI CTAG 4 cut(s) 329, 486, 567, 737
MaeII ACGT 2 cut(s) 895, 901
MalI GATC 2 cut(s) 282, 294
MboI GATC 2 cut(s) 280, 292
MboII GAAGA 8 cut(s) 332, 801, 820, 1003, 1057, 1192, 1273, 1347
MfeI CAATTG 1 cut(s) 12
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 2 cut(s) 910, 949
MluCI AATT 3 cut(s) 12, 752, 1125
MluNI TGGCCA 2 cut(s) 910, 949
MlyI GAGTC 4 cut(s) 517, 768, 1012, 1216
MmeI TCCRAC 1 cut(s) 183
Mox20I TGGCCA 2 cut(s) 910, 949
MroXI GAANNNNTTC 1 cut(s) 756
MscI TGGCCA 2 cut(s) 910, 949
MseI TTAA 3 cut(s) 237, 975, 1152
Msp20I TGGCCA 2 cut(s) 910, 949
MspA1I CMGCKG 1 cut(s) 1144
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 5 cut(s) 395, 688, 1022, 1199, 1325
MspR9I CCNGG 2 cut(s) 396, 539
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 1 cut(s) 539
MvnI CGCG 3 cut(s) 709, 1138, 1144
MwoI GCNNNNNNNGC 4 cut(s) 253, 482, 786, 1094
NciI CCSGG 1 cut(s) 396
NdeII GATC 2 cut(s) 280, 292
NlaIII CATG 3 cut(s) 98, 165, 1297
NlaIV GGNNCC 2 cut(s) 1148, 1203
NmeAIII GCCGAG 3 cut(s) 835, 1195, 1230
NspI RCATGY 1 cut(s) 1297
PaeI GCATGC 1 cut(s) 1297
PaeR7I CTCGAG 1 cut(s) 573
PciSI GCTCTTC 2 cut(s) 833, 986
PdmI GAANNNNTTC 1 cut(s) 756
PfeI GAWTC 5 cut(s) 39, 557, 637, 757, 1193
PflFI GACNNNGTC 1 cut(s) 1267
PflMI CCANNNNNTGG 2 cut(s) 167, 472
PfoI TCCNGGA 1 cut(s) 537
PkrI GCNGC 4 cut(s) 588, 726, 825, 1292
PleI GAGTC 4 cut(s) 517, 768, 1012, 1215
PpsI GAGTC 4 cut(s) 517, 768, 1012, 1215
PshAI GACNNNNGTC 1 cut(s) 1367
PshBI ATTAAT 1 cut(s) 975
Psp124BI GAGCTC 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 537
PspGI CCWGG 1 cut(s) 537
PspN4I GGNNCC 2 cut(s) 1148, 1203
PspPI GGNCC 2 cut(s) 685, 926
PstNI CAGNNNCTG 1 cut(s) 1253
PsyI GACNNNGTC 1 cut(s) 1267
RsaI GTAC 5 cut(s) 342, 374, 432, 565, 571
RsaNI GTAC 5 cut(s) 341, 373, 431, 564, 570
SacI GAGCTC 1 cut(s) 258
SacII CCGCGG 1 cut(s) 1145
SalI GTCGAC 2 cut(s) 897, 1370
SapI GCTCTTC 2 cut(s) 833, 986
SaqAI TTAA 3 cut(s) 237, 975, 1152
SatI GCNGC 4 cut(s) 587, 725, 824, 1291
Sau3AI GATC 2 cut(s) 280, 292
Sau96I GGNCC 2 cut(s) 685, 926
ScaI AGTACT 1 cut(s) 571
SchI GAGTC 4 cut(s) 517, 768, 1012, 1216
ScrFI CCNGG 2 cut(s) 396, 539
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 3 cut(s) 145, 408, 1222
SfcI CTRYAG 1 cut(s) 764
Sfr274I CTCGAG 1 cut(s) 573
Sfr303I CCGCGG 1 cut(s) 1145
SgrBI CCGCGG 1 cut(s) 1145
SgrDI CGTCGACG 1 cut(s) 897
SinI GGWCC 1 cut(s) 685
SlaI CTCGAG 1 cut(s) 573
SmlI CTYRAG 5 cut(s) 236, 349, 573, 718, 1049
SmoI CTYRAG 5 cut(s) 236, 349, 573, 718, 1049
SpeI ACTAGT 1 cut(s) 566
SphI GCATGC 1 cut(s) 1297
Sse9I AATT 3 cut(s) 12, 752, 1125
SsiI CCGC 8 cut(s) 31, 778, 823, 1136, 1142, 1144, 1291, 1322
SspI AATATT 2 cut(s) 973, 1160
SspMI CTAG 4 cut(s) 329, 486, 567, 737
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 2 cut(s) 394, 537
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 3 cut(s) 210, 1043, 1366
TaiI ACGT 2 cut(s) 898, 904
TaqI TCGA 6 cut(s) 574, 635, 671, 898, 1271, 1371
TaqII GACCGA 2 cut(s) 795, 905
TasI AATT 3 cut(s) 12, 752, 1125
TatI WGTACW 3 cut(s) 372, 563, 569
TauI GCSGC 2 cut(s) 826, 1293
TfiI GAWTC 5 cut(s) 39, 557, 637, 757, 1193
Tru1I TTAA 3 cut(s) 237, 975, 1152
Tru9I TTAA 3 cut(s) 237, 975, 1152
TscAI CASTG 3 cut(s) 215, 417, 1048
TseI GCWGC 2 cut(s) 586, 724
TspDTI ATGAA 5 cut(s) 39, 146, 333, 586, 749
TspRI CASTG 3 cut(s) 215, 417, 1048
Tth111I GACNNNGTC 1 cut(s) 1267
Van91I CCANNNNNTGG 2 cut(s) 167, 472
Vha464I CTTAAG 1 cut(s) 236
VpaK11BI GGWCC 1 cut(s) 685
VspI ATTAAT 1 cut(s) 975
XceI RCATGY 1 cut(s) 1297
XhoI CTCGAG 1 cut(s) 573
XmiI GTMKAC 2 cut(s) 898, 1371
XmnI GAANNNNTTC 1 cut(s) 756
XspI CTAG 4 cut(s) 329, 486, 567, 737
ZrmI AGTACT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.