Rmu_ssc0000354.1_g000001

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000354.1
OG0014835
Physical Location & Seq
Reverse (-)
933 .. 1577
645 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
atgtctggagttacatgctgcctaaggttccctggacagcttaactctgatttgcgcaagcttgctgttaacctcatccccttccctcgtcttcactttttcatggtcggttttgctcctctgacctcaagagggtctcagcagtacagagctttgactgtcccagaactcacccagcaaatgtgggattccaagaacatgatgtgtgctgctgatccccgacatggaaggtacctcacagcatcagccatgttccgtggtaagatgagcaccaaggaagtggatgaccagatgatgaatgtgcagaacaagaactcttcctactttgttgagtggatccccaacaacgtcaagtcaacagtctgtgacatcccaccaactggtctcaagatggcttcaactttcatcggaaactctacatccattcaagagatgttcaggagggtgagtgagcagttcactgctatgttcaggaggaaggctttcttgcattggtacactggtgaaggcatggacgagatggaattcactgaagctgagagcaacatgaatgatctggtgtcagaatatcagcagtaccaggatgccaccgctgatgaggacgagtatgaagatgaacagggagagtatgaggaggaaatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

214

Amino Acids

24.73

Weight (kDa)

4.77

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin_C PF03953 29 - 150 3.1e-41 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 56
Acc65I GGTACC 1 cut(s) 231
AccB1I GGYRCC 1 cut(s) 231
AccB7I CCANNNNNTGG 1 cut(s) 380
AciI CCGC 1 cut(s) 591
AclWI GGATC 3 cut(s) 209, 331, 344
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 1 cut(s) 552
AfaI GTAC 4 cut(s) 146, 233, 497, 578
AfiI CCNNNNNNNGG 3 cut(s) 132, 224, 380
AgsI TTSAA 2 cut(s) 399, 428
AjnI CCWGG 2 cut(s) 31, 579
AluBI AGCT 4 cut(s) 40, 61, 152, 536
AluI AGCT 4 cut(s) 40, 61, 152, 536
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 2 cut(s) 141, 389
AlwI GGATC 3 cut(s) 209, 331, 344
ApeKI GCWGC 2 cut(s) 18, 209
ApoI RAATTY 1 cut(s) 524
Asp700I GAANNNNTTC 1 cut(s) 482
Asp718I GGTACC 1 cut(s) 231
AspLEI GCGC 1 cut(s) 57
AsuHPI GGTGA 3 cut(s) 163, 457, 515
AxyI CCTNAGG 1 cut(s) 23
BaeI ACNNNNGTAYC 2 cut(s) 487, 520
BamHI GGATCC 1 cut(s) 336
BanI GGYRCC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 272
BbvI GCAGC 2 cut(s) 5, 196
BccI CCATC 2 cut(s) 385, 514
BciT130I CCWGG 2 cut(s) 33, 581
BcoDI GTCTC 2 cut(s) 141, 389
BisI GCNGC 2 cut(s) 19, 210
BlsI GCNGC 2 cut(s) 20, 211
Bme1390I CCNGG 2 cut(s) 33, 581
BmiI GGNNCC 3 cut(s) 29, 233, 338
BmrFI CCNGG 2 cut(s) 33, 581
BmsI GCATC 2 cut(s) 251, 574
BpiI GAAGAC 1 cut(s) 83
BpmI CTGGAG 1 cut(s) 27
BpuEI CTTGAG 2 cut(s) 112, 371
BsaI GGTCTC 2 cut(s) 141, 389
BsaJI CCNNGG 3 cut(s) 31, 256, 273
BsaXI ACNNNNNCTCC 3 cut(s) 433, 463, 626
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 224, 380
Bse1I ACTGG 2 cut(s) 385, 505
Bse21I CCTNAGG 1 cut(s) 23
BseBI CCWGG 2 cut(s) 33, 581
BseDI CCNNGG 3 cut(s) 31, 256, 273
BseGI GGATG 5 cut(s) 75, 289, 369, 419, 589
BseLI CCNNNNNNNGG 3 cut(s) 132, 224, 380
BseMII CTCAG 2 cut(s) 152, 528
BseNI ACTGG 2 cut(s) 385, 505
BseRI GAGGAG 1 cut(s) 108
BseXI GCAGC 2 cut(s) 5, 196
BseYI CCCAGC 1 cut(s) 174
BsgI GTGCAG 1 cut(s) 323
BshNI GGYRCC 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 272
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 3 cut(s) 132, 224, 380
BsmAI GTCTC 2 cut(s) 141, 389
BsmFI GGGAC 1 cut(s) 146
Bso31I GGTCTC 2 cut(s) 141, 389
Bsp1286I GDGCHC 1 cut(s) 272
Bsp143I GATC 3 cut(s) 214, 336, 553
BspACI CCGC 1 cut(s) 591
BspCNI CTCAG 2 cut(s) 151, 529
BspLI GGNNCC 3 cut(s) 29, 233, 338
BspPI GGATC 3 cut(s) 209, 331, 344
BspT107I GGYRCC 1 cut(s) 231
BspTNI GGTCTC 2 cut(s) 141, 389
BsrI ACTGG 2 cut(s) 385, 505
BssECI CCNNGG 3 cut(s) 31, 256, 273
BssMI GATC 3 cut(s) 214, 336, 553
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 2 cut(s) 33, 581
Bst4CI ACNGT 2 cut(s) 160, 361
Bst6I CTCTTC 1 cut(s) 322
BstC8I GCNNGC 2 cut(s) 59, 63
BstDEI CTNAG 3 cut(s) 23, 138, 537
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 5 cut(s) 75, 289, 369, 419, 589
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 3 cut(s) 217, 339, 556
BstMAI GTCTC 2 cut(s) 141, 389
BstMBI GATC 3 cut(s) 214, 336, 553
BstNI CCWGG 2 cut(s) 33, 581
BstNSI RCATGY 1 cut(s) 18
BstSCI CCNGG 2 cut(s) 31, 579
BstV1I GCAGC 2 cut(s) 5, 196
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 1 cut(s) 336
BstXI CCANNNNNNTGG 1 cut(s) 280
BstYI RGATCY 1 cut(s) 336
Bsu36I CCTNAGG 1 cut(s) 23
BtgI CCRYGG 1 cut(s) 256
BtsCI GGATG 5 cut(s) 75, 289, 369, 419, 589
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 3 cut(s) 459, 498, 528
Cac8I GCNNGC 2 cut(s) 59, 63
CfoI GCGC 1 cut(s) 57
Csp6I GTAC 4 cut(s) 145, 232, 496, 577
CviAII CATG 7 cut(s) 15, 103, 199, 224, 250, 511, 547
CviJI RGCY 7 cut(s) 40, 61, 152, 248, 395, 482, 536
CviKI_1 RGCY 7 cut(s) 40, 61, 152, 248, 395, 482, 536
CviQI GTAC 4 cut(s) 145, 232, 496, 577
DdeI CTNAG 3 cut(s) 23, 138, 537
DpnI GATC 3 cut(s) 216, 338, 555
DpnII GATC 3 cut(s) 214, 336, 553
Eam1104I CTCTTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 273
Eco31I GGTCTC 2 cut(s) 141, 389
Eco57I CTGAAG 1 cut(s) 552
Eco81I CCTNAGG 1 cut(s) 23
EcoRI GAATTC 1 cut(s) 524
EcoRII CCWGG 2 cut(s) 31, 579
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 7 cut(s) 18, 106, 202, 227, 253, 514, 550
FalI AAGNNNNNCTT 2 cut(s) 470, 502
FaqI GGGAC 1 cut(s) 146
FatI CATG 7 cut(s) 14, 102, 198, 223, 249, 510, 546
Fnu4HI GCNGC 2 cut(s) 19, 210
FokI GGATG 5 cut(s) 62, 296, 356, 406, 596
Fsp4HI GCNGC 2 cut(s) 19, 210
FspI TGCGCA 1 cut(s) 56
GlaI GCGC 1 cut(s) 56
GluI GCNGC 2 cut(s) 19, 210
GsaI CCCAGC 1 cut(s) 178
GsuI CTGGAG 1 cut(s) 27
HhaI GCGC 1 cut(s) 57
Hin1II CATG 7 cut(s) 18, 106, 202, 227, 253, 514, 550
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HincII GTYRAC 2 cut(s) 70, 357
HindII GTYRAC 2 cut(s) 70, 357
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 1 cut(s) 188
HpaI GTTAAC 1 cut(s) 70
HphI GGTGA 3 cut(s) 163, 457, 515
Hpy166II GTNNAC 4 cut(s) 70, 357, 459, 498
Hpy188I TCNGA 4 cut(s) 49, 123, 410, 565
Hpy188III TCNNGA 6 cut(s) 6, 129, 388, 428, 439, 472
Hpy8I GTNNAC 4 cut(s) 70, 357, 459, 498
HpyAV CCTTC 4 cut(s) 91, 222, 472, 500
HpyCH4III ACNGT 2 cut(s) 160, 361
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 2 cut(s) 304, 490
HpyF3I CTNAG 3 cut(s) 23, 138, 537
HpySE526I ACGT 1 cut(s) 348
Hsp92II CATG 7 cut(s) 18, 106, 202, 227, 253, 514, 550
HspAI GCGC 1 cut(s) 55
KpnI GGTACC 1 cut(s) 235
KspAI GTTAAC 1 cut(s) 70
Kzo9I GATC 3 cut(s) 214, 336, 553
LmnI GCTCC 1 cut(s) 121
Lsp1109I GCAGC 2 cut(s) 5, 196
LweI GCATC 2 cut(s) 251, 574
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 10, 365
MalI GATC 3 cut(s) 216, 338, 555
MboI GATC 3 cut(s) 214, 336, 553
MboII GAAGA 3 cut(s) 83, 309, 623
MflI RGATCY 1 cut(s) 336
MhlI GDGCHC 1 cut(s) 272
MluCI AATT 1 cut(s) 524
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 2 cut(s) 42, 69
MslI CAYNNNNRTG 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 593
MspR9I CCNGG 2 cut(s) 33, 581
MvaI CCWGG 2 cut(s) 33, 581
NdeII GATC 3 cut(s) 214, 336, 553
NlaIII CATG 7 cut(s) 18, 106, 202, 227, 253, 514, 550
NlaIV GGNNCC 3 cut(s) 29, 233, 338
NmuCI GTSAC 1 cut(s) 365
NsbI TGCGCA 1 cut(s) 56
NspI RCATGY 1 cut(s) 18
PdmI GAANNNNTTC 1 cut(s) 482
PfeI GAWTC 1 cut(s) 188
PflMI CCANNNNNTGG 1 cut(s) 380
PkrI GCNGC 2 cut(s) 20, 211
Psp6I CCWGG 2 cut(s) 31, 579
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 2 cut(s) 31, 579
PspN4I GGNNCC 3 cut(s) 29, 233, 338
PsrI GAACNNNNNNTAC 2 cut(s) 305, 337
PsuI RGATCY 1 cut(s) 336
RsaI GTAC 4 cut(s) 146, 233, 497, 578
RsaNI GTAC 4 cut(s) 145, 232, 496, 577
RseI CAYNNNNRTG 1 cut(s) 464
SaqAI TTAA 2 cut(s) 42, 69
SatI GCNGC 2 cut(s) 19, 210
Sau3AI GATC 3 cut(s) 214, 336, 553
ScrFI CCNGG 2 cut(s) 33, 581
SduI GDGCHC 1 cut(s) 272
SfaNI GCATC 2 cut(s) 251, 574
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 2 cut(s) 127, 386
SmoI CTYRAG 2 cut(s) 127, 386
Sse9I AATT 1 cut(s) 524
SsiI CCGC 1 cut(s) 591
StyD4I CCNGG 2 cut(s) 31, 579
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 2 cut(s) 160, 361
TaiI ACGT 1 cut(s) 351
TasI AATT 1 cut(s) 524
TatI WGTACW 1 cut(s) 144
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 2 cut(s) 42, 69
Tru9I TTAA 2 cut(s) 42, 69
TscAI CASTG 3 cut(s) 466, 505, 535
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 2 cut(s) 18, 209
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 6 cut(s) 91, 311, 394, 563, 624, 630
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 3 cut(s) 466, 505, 535
Van91I CCANNNNNTGG 1 cut(s) 380
XapI RAATTY 1 cut(s) 524
XceI RCATGY 1 cut(s) 18
XmnI GAANNNNTTC 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.