Rmu_ssc0000468.1_g000007

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000468.1
OG0005131
Physical Location & Seq
Reverse (-)
37176 .. 38231
1056 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1056 bp
ctgagagggccgatcgaaatccacccaccgtcgcggccagatggtctgaccccccaaacaacagaccctcttcttcctctctggatcacggtttgggcccaagttggaatctgcccgagccgtgaaacgatcatggcagctctaatgtaccagatattgtcgtcctcagcgctggtatctctgggactctatcatatggtggccaccacccgcaactacctgaaatctccccagtcctattccgctaagccctatcatccctttcctctctcatcttcttcccctaatcatcgcctcaggtacgtccagctgttcctcatcaccgcctgccttatcgtcgccgtcgtccaccaggcggcggcctccttggacgctgacccactcctcaagggccgcacccctgtccaccgcttcacctccctccagtccgccgctgctctcttcctctttctgctcctcacgctcgccatcctcctctccgagtgcgccccttctctgctccccctctccaccgatctcatcttcggcctcgccgccgcgctcttctacatccactccttcatctctcgggaagctgcctccgtccagacctccgatctgcaggccaagtgcgattccgtctccggccgggtctccgcactggcctccctgctctgccttgtcttggcctgccagcccaagctctttgttgctgatgtgggtttgggcgctgccatgtccctgcagggcctctgggtcttgcagaccgggctctccctttacgcggatgccttcatccctgagggctgccacaagctacttgatgtcgtcagcggggttgagggttcaaccaagtgcgacctggatgagtcccgcttcagggcagttgctattttggatctcttgtttctggtgcacgtcatgtttgtgttgctcattatgctggtcgtccatgcagtggttgccaagtctgttggcatccgcaagttggcttcttacgaggccttgcctaccgctgtgcccgacaacaaccacaatcatattcagatgaaagccttgagcggcacccaggcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

351

Amino Acids

37.82

Weight (kDa)

7.77

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 172 - 310 5.6e-31 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1043
AccB7I CCANNNNNTGG 1 cut(s) 937
AccBSI CCGCTC 1 cut(s) 1041
AccII CGCG 3 cut(s) 34, 539, 764
AclWI GGATC 2 cut(s) 92, 885
AcoI YGGCCR 3 cut(s) 35, 201, 625
AcuI CTGAAG 1 cut(s) 841
AfaI GTAC 2 cut(s) 149, 302
AfeI AGCGCT 1 cut(s) 171
AfiI CCNNNNNNNGG 8 cut(s) 355, 358, 664, 763, 858, 859, 937, 967
AgsI TTSAA 1 cut(s) 828
AjiI CACGTC 1 cut(s) 898
AjnI CCWGG 3 cut(s) 351, 840, 1047
AluBI AGCT 5 cut(s) 140, 310, 575, 682, 796
AluI AGCT 5 cut(s) 140, 310, 575, 682, 796
Alw21I GWGCWC 1 cut(s) 897
Alw26I GTCTC 2 cut(s) 625, 637
Alw44I GTGCAC 1 cut(s) 893
AlwI GGATC 2 cut(s) 92, 885
Ama87I CYCGRG 2 cut(s) 115, 567
Aor51HI AGCGCT 1 cut(s) 171
ApaI GGGCCC 1 cut(s) 100
ApaLI GTGCAC 1 cut(s) 893
ApeKI GCWGC 5 cut(s) 137, 434, 575, 710, 786
AspLEI GCGC 4 cut(s) 172, 488, 541, 710
AspS9I GGNCC 5 cut(s) 8, 96, 97, 391, 727
AsuC2I CCSGG 2 cut(s) 629, 748
AsuHPI GGTGA 2 cut(s) 313, 406
AvaI CYCGRG 2 cut(s) 115, 567
AxyI CCTNAGG 2 cut(s) 296, 780
BaeGI GKGCMC 3 cut(s) 100, 897, 1002
BalI TGGCCA 1 cut(s) 203
BanI GGYRCC 1 cut(s) 1043
BanII GRGCYC 2 cut(s) 100, 753
Bbv12I GWGCWC 1 cut(s) 897
BbvCI CCTCAGC 1 cut(s) 166
BbvI GCAGC 5 cut(s) 149, 421, 562, 697, 773
BccI CCATC 2 cut(s) 35, 476
BceAI ACGGC 2 cut(s) 105, 326
BciT130I CCWGG 3 cut(s) 353, 842, 1049
BcnI CCSGG 2 cut(s) 629, 748
BcoDI GTCTC 2 cut(s) 625, 637
BfmI CTRYAG 2 cut(s) 599, 722
BfoI RGCGCY 2 cut(s) 173, 711
BlpI GCTNAGC 1 cut(s) 246
Bme1390I CCNGG 5 cut(s) 353, 629, 748, 842, 1049
BmeT110I CYCGRG 2 cut(s) 115, 567
BmgBI CACGTC 1 cut(s) 898
BmgT120I GGNCC 5 cut(s) 8, 96, 97, 391, 727
BmiI GGNNCC 2 cut(s) 98, 1045
BmrFI CCNGG 5 cut(s) 353, 629, 748, 842, 1049
BmrI ACTGGG 1 cut(s) 226
BmsI GCATC 2 cut(s) 757, 966
BmuI ACTGGG 1 cut(s) 226
BpmI CTGGAG 1 cut(s) 407
Bpu10I CCTNAGC 1 cut(s) 166
Bpu1102I GCTNAGC 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 2 cut(s) 629, 748
BsaBI GATNNNNATC 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 637
BsaJI CCNNGG 2 cut(s) 366, 1047
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bsc4I CCNNNNNNNGG 8 cut(s) 355, 358, 664, 763, 858, 859, 937, 967
Bse1I ACTGG 3 cut(s) 232, 424, 645
Bse21I CCTNAGG 2 cut(s) 296, 780
Bse8I GATNNNNATC 1 cut(s) 17
BseBI CCWGG 3 cut(s) 353, 842, 1049
BseDI CCNNGG 2 cut(s) 366, 1047
BseGI GGATG 7 cut(s) 256, 468, 549, 772, 774, 850, 957
BseJI GATNNNNATC 1 cut(s) 17
BseLI CCNNNNNNNGG 8 cut(s) 355, 358, 664, 763, 858, 859, 937, 967
BseMII CTCAG 3 cut(s) 180, 310, 771
BseNI ACTGG 3 cut(s) 232, 424, 645
BseRI GAGGAG 3 cut(s) 374, 446, 464
BseSI GKGCMC 3 cut(s) 100, 897, 1002
BseX3I CGGCCG 1 cut(s) 625
BseXI GCAGC 5 cut(s) 149, 421, 562, 697, 773
Bsh1236I CGCG 3 cut(s) 34, 539, 764
Bsh1285I CGRYCG 2 cut(s) 15, 628
BshNI GGYRCC 1 cut(s) 1043
BsiEI CGRYCG 2 cut(s) 15, 628
BsiHKAI GWGCWC 1 cut(s) 897
BsiHKCI CYCGRG 2 cut(s) 115, 567
BsiSI CCGG 3 cut(s) 624, 628, 747
BslFI GGGAC 3 cut(s) 198, 703, 835
BslI CCNNNNNNNGG 8 cut(s) 355, 358, 664, 763, 858, 859, 937, 967
BsmAI GTCTC 2 cut(s) 625, 637
BsmBI CGTCTC 1 cut(s) 625
BsmFI GGGAC 3 cut(s) 198, 703, 835
Bso31I GGTCTC 1 cut(s) 637
BsoBI CYCGRG 2 cut(s) 115, 567
Bsp120I GGGCCC 1 cut(s) 96
Bsp1286I GDGCHC 4 cut(s) 100, 753, 897, 1002
Bsp143I GATC 6 cut(s) 12, 84, 129, 514, 595, 877
Bsp1720I GCTNAGC 1 cut(s) 246
BspCNI CTCAG 3 cut(s) 179, 309, 772
BspFNI CGCG 3 cut(s) 34, 539, 764
BspLI GGNNCC 2 cut(s) 98, 1045
BspMAI CTGCAG 2 cut(s) 603, 726
BspPI GGATC 2 cut(s) 92, 885
BspQI GCTCTTC 1 cut(s) 548
BspT107I GGYRCC 1 cut(s) 1043
BspTNI GGTCTC 1 cut(s) 637
BsrBI CCGCTC 1 cut(s) 1041
BsrI ACTGG 3 cut(s) 232, 424, 645
BssECI CCNNGG 2 cut(s) 366, 1047
BssMI GATC 6 cut(s) 12, 84, 129, 514, 595, 877
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 3 cut(s) 353, 842, 1049
Bst4CI ACNGT 2 cut(s) 30, 91
Bst6I CTCTTC 3 cut(s) 75, 446, 548
BstAPI GCANNNNNTGC 1 cut(s) 941
BstC8I GCNNGC 5 cut(s) 328, 465, 603, 670, 674
BstDEI CTNAG 6 cut(s) 2, 166, 246, 296, 780, 1053
BstF5I GGATG 7 cut(s) 256, 468, 549, 772, 774, 850, 957
BstFNI CGCG 3 cut(s) 34, 539, 764
BstH2I RGCGCY 2 cut(s) 173, 711
BstHHI GCGC 4 cut(s) 172, 488, 541, 710
BstKTI GATC 6 cut(s) 15, 87, 132, 517, 598, 880
BstMAI GTCTC 2 cut(s) 625, 637
BstMBI GATC 6 cut(s) 12, 84, 129, 514, 595, 877
BstMCI CGRYCG 2 cut(s) 15, 628
BstMWI GCNNNNNNNGC 4 cut(s) 460, 748, 919, 941
BstNI CCWGG 3 cut(s) 353, 842, 1049
BstSCI CCNGG 5 cut(s) 351, 627, 746, 840, 1047
BstSFI CTRYAG 2 cut(s) 599, 722
BstSLI GKGCMC 3 cut(s) 100, 897, 1002
BstUI CGCG 3 cut(s) 34, 539, 764
BstV1I GCAGC 5 cut(s) 149, 421, 562, 697, 773
BstX2I RGATCY 1 cut(s) 877
BstYI RGATCY 1 cut(s) 877
BstZI CGGCCG 1 cut(s) 625
Bsu36I CCTNAGG 2 cut(s) 296, 780
BtgZI GCGATG 1 cut(s) 275
BtrI CACGTC 1 cut(s) 898
BtsCI GGATG 7 cut(s) 256, 468, 549, 772, 774, 850, 957
BtsI GCAGTG 1 cut(s) 942
BtsIMutI CAGTG 2 cut(s) 638, 942
Cac8I GCNNGC 5 cut(s) 328, 465, 603, 670, 674
CfoI GCGC 4 cut(s) 172, 488, 541, 710
Cfr13I GGNCC 5 cut(s) 8, 96, 97, 391, 727
CseI GACGC 1 cut(s) 380
Csp6I GTAC 2 cut(s) 148, 301
CviAII CATG 4 cut(s) 133, 715, 901, 932
CviQI GTAC 2 cut(s) 148, 301
DdeI CTNAG 6 cut(s) 2, 166, 246, 296, 780, 1053
DpnI GATC 6 cut(s) 14, 86, 131, 516, 597, 879
DpnII GATC 6 cut(s) 12, 84, 129, 514, 595, 877
EaeI YGGCCR 3 cut(s) 35, 201, 625
EagI CGGCCG 1 cut(s) 625
Eam1104I CTCTTC 3 cut(s) 75, 446, 548
EarI CTCTTC 3 cut(s) 75, 446, 548
EciI GGCGGA 1 cut(s) 418
EclXI CGGCCG 1 cut(s) 625
Eco130I CCWWGG 1 cut(s) 366
Eco147I AGGCCT 1 cut(s) 983
Eco24I GRGCYC 2 cut(s) 100, 753
Eco31I GGTCTC 1 cut(s) 637
Eco47III AGCGCT 1 cut(s) 171
Eco52I CGGCCG 1 cut(s) 625
Eco57I CTGAAG 1 cut(s) 841
Eco81I CCTNAGG 2 cut(s) 296, 780
Eco88I CYCGRG 2 cut(s) 115, 567
EcoO109I RGGNCCY 1 cut(s) 727
EcoRII CCWGG 3 cut(s) 351, 840, 1047
EcoT14I CCWWGG 1 cut(s) 366
EcoT38I GRGCYC 2 cut(s) 100, 753
ErhI CCWWGG 1 cut(s) 366
Esp3I CGTCTC 1 cut(s) 625
FaeI CATG 4 cut(s) 136, 718, 904, 935
FaiI YATR 8 cut(s) 134, 195, 197, 716, 902, 920, 933, 1020
FaqI GGGAC 3 cut(s) 198, 703, 835
FatI CATG 4 cut(s) 132, 714, 900, 931
FauI CCCGC 3 cut(s) 218, 806, 860
FauNDI CATATG 1 cut(s) 195
FokI GGATG 7 cut(s) 243, 455, 536, 761, 779, 857, 944
FriOI GRGCYC 2 cut(s) 100, 753
GlaI GCGC 4 cut(s) 171, 487, 540, 709
GsuI CTGGAG 1 cut(s) 407
HaeII RGCGCY 2 cut(s) 173, 711
HapII CCGG 3 cut(s) 624, 628, 747
HgaI GACGC 1 cut(s) 380
HhaI GCGC 4 cut(s) 172, 488, 541, 710
Hin1II CATG 4 cut(s) 136, 718, 904, 935
Hin6I GCGC 4 cut(s) 170, 486, 539, 708
HinP1I GCGC 4 cut(s) 170, 486, 539, 708
HinfI GANTC 4 cut(s) 108, 186, 614, 848
HpaII CCGG 3 cut(s) 624, 628, 747
HphI GGTGA 2 cut(s) 313, 406
Hpy166II GTNNAC 3 cut(s) 349, 406, 895
Hpy188I TCNGA 4 cut(s) 48, 481, 595, 1026
Hpy188III TCNNGA 3 cut(s) 82, 569, 586
Hpy8I GTNNAC 3 cut(s) 349, 406, 895
Hpy99I CGWCG 3 cut(s) 34, 341, 347
HpyAV CCTTC 3 cut(s) 501, 568, 781
HpyCH4III ACNGT 2 cut(s) 30, 91
HpyCH4IV ACGT 2 cut(s) 303, 897
HpyCH4V TGCA 5 cut(s) 601, 724, 742, 895, 935
HpyF10VI GCNNNNNNNGC 4 cut(s) 460, 748, 919, 941
HpyF3I CTNAG 6 cut(s) 2, 166, 246, 296, 780, 1053
HpySE526I ACGT 2 cut(s) 303, 897
Hsp92II CATG 4 cut(s) 136, 718, 904, 935
HspAI GCGC 4 cut(s) 170, 486, 539, 708
Kzo9I GATC 6 cut(s) 12, 84, 129, 514, 595, 877
LguI GCTCTTC 1 cut(s) 548
LmnI GCTCC 2 cut(s) 459, 504
Lsp1109I GCAGC 5 cut(s) 149, 421, 562, 697, 773
LweI GCATC 2 cut(s) 757, 966
MaeII ACGT 2 cut(s) 303, 897
MalI GATC 6 cut(s) 14, 86, 131, 516, 597, 879
MbiI CCGCTC 1 cut(s) 1041
MboI GATC 6 cut(s) 12, 84, 129, 514, 595, 877
MboII GAAGA 7 cut(s) 62, 65, 267, 270, 433, 514, 535
MflI RGATCY 1 cut(s) 877
MhlI GDGCHC 4 cut(s) 100, 753, 897, 1002
MlsI TGGCCA 1 cut(s) 203
MluNI TGGCCA 1 cut(s) 203
MlyI GAGTC 2 cut(s) 180, 857
MmeI TCCRAC 1 cut(s) 85
Mox20I TGGCCA 1 cut(s) 203
MscI TGGCCA 1 cut(s) 203
MslI CAYNNNNRTG 1 cut(s) 905
Msp20I TGGCCA 1 cut(s) 203
MspA1I CMGCKG 4 cut(s) 310, 434, 813, 995
MspI CCGG 3 cut(s) 624, 628, 747
MspR9I CCNGG 5 cut(s) 353, 629, 748, 842, 1049
MvaI CCWGG 3 cut(s) 353, 842, 1049
MvnI CGCG 3 cut(s) 34, 539, 764
MwoI GCNNNNNNNGC 4 cut(s) 460, 748, 919, 941
NciI CCSGG 2 cut(s) 629, 748
NdeI CATATG 1 cut(s) 195
NdeII GATC 6 cut(s) 12, 84, 129, 514, 595, 877
NlaIII CATG 4 cut(s) 136, 718, 904, 935
NlaIV GGNNCC 2 cut(s) 98, 1045
PceI AGGCCT 1 cut(s) 983
PciSI GCTCTTC 1 cut(s) 548
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PfeI GAWTC 2 cut(s) 108, 614
PflMI CCANNNNNTGG 1 cut(s) 937
Ple19I CGATCG 1 cut(s) 15
PleI GAGTC 2 cut(s) 180, 856
PpsI GAGTC 2 cut(s) 180, 856
Psp6I CCWGG 3 cut(s) 351, 840, 1047
PspGI CCWGG 3 cut(s) 351, 840, 1047
PspN4I GGNNCC 2 cut(s) 98, 1045
PspOMI GGGCCC 1 cut(s) 96
PspPI GGNCC 5 cut(s) 8, 96, 97, 391, 727
PstI CTGCAG 2 cut(s) 603, 726
PsuI RGATCY 1 cut(s) 877
PvuI CGATCG 1 cut(s) 15
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 2 cut(s) 149, 302
RsaNI GTAC 2 cut(s) 148, 301
RseI CAYNNNNRTG 1 cut(s) 905
SapI GCTCTTC 1 cut(s) 548
Sau3AI GATC 6 cut(s) 12, 84, 129, 514, 595, 877
Sau96I GGNCC 5 cut(s) 8, 96, 97, 391, 727
SbfI CCTGCAGG 1 cut(s) 726
SchI GAGTC 2 cut(s) 180, 857
ScrFI CCNGG 5 cut(s) 353, 629, 748, 842, 1049
SdaI CCTGCAGG 1 cut(s) 726
SduI GDGCHC 4 cut(s) 100, 753, 897, 1002
SfaNI GCATC 2 cut(s) 757, 966
SfcI CTRYAG 2 cut(s) 599, 722
SmiMI CAYNNNNRTG 1 cut(s) 905
SmlI CTYRAG 2 cut(s) 386, 1036
SmoI CTYRAG 2 cut(s) 386, 1036
Sse8387I CCTGCAGG 1 cut(s) 726
SseBI AGGCCT 1 cut(s) 983
StuI AGGCCT 1 cut(s) 983
StyD4I CCNGG 5 cut(s) 351, 627, 746, 840, 1047
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 2 cut(s) 30, 91
TaiI ACGT 2 cut(s) 306, 900
TaqI TCGA 1 cut(s) 15
TauI GCSGC 8 cut(s) 37, 359, 362, 396, 434, 536, 539, 1044
TfiI GAWTC 2 cut(s) 108, 614
TscAI CASTG 2 cut(s) 645, 942
TseI GCWGC 5 cut(s) 137, 434, 575, 710, 786
TspDTI ATGAA 3 cut(s) 550, 763, 1043
TspGWI ACGGA 2 cut(s) 571, 607
TspRI CASTG 2 cut(s) 645, 942
Van91I CCANNNNNTGG 1 cut(s) 937
VneI GTGCAC 1 cut(s) 893
XcmI CCANNNNNNNNNTGG 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.