Rroxscaffold_1G00005130
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB NOP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010493
Physical Location & Seq
Reverse (-)
6910255 .. 6915839
5585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00005130.1

Sequence Viewer

Length: 1086 bp
ATGCCACCGTATGCTGTTGTGGATGAGAATGTGAGGCTTGCGAAGATTGCCCTTAGACATGGAGCTGGCAACATGGTCAATGGGATTCTCCGGAAGCTTGTTCTACTTAAGGAAAGTAACTCTCTTCCTCTACCTTGGGTAGAGGGTGATGATCGCACACAAGCACGTGCTCTTGCCACTCTGTACTCTCATCCTGTATGGATGATAAGGCGATGGACAAAGCATCTGGGACAAGAAGAAGCAATTAGATTGATGGCATGGAATAATAGTGATCCTAGTTTCAGCTTAAGGGCAAACAGTGGGAAAGGTATTTCAAGAGCTGACCTTGTGACAAAGCTTAATTTATTGGAGGTCCCACATGAGCTCTCTTTGCATTTGAATGATTTTGTCCGTATGAAAAAAGGGTTGCAGATTGTAATTCAAGCTGGTTTGCTAAAAGAAGGTTTATGTTCTGTACAGGACGAGAGTGCAGGTCTGGTAGTTTCTGTTGTAGATCCGCAACAGGGTGAAAGCATTATTGATTGTTGTGCTGCTCCTGGAGGAAAGACTCTTTACATGGCGTCCCTTTTGAGTGGCCAAGGAATGGTATATGCAATTGACATAAATGAAGGGCGGTTGAGAATCCTTAAAGAAACGGCTAAGTTGCACCAAGTAGATGGTGTCGTCACCACTATCCATGCTGATATGCGTAGTTATGCTGACAATAATACTATGAAGTGTGATAAAGTTCTGTTGGATGCTCCATGTTCTGGACTGGGTGTTCTCTCCAAGAGGGCAGACTTGCGTTGGAACCGGAGATTAGAGGATATGGAGGAGCTTAAGAATTTGCAGGATGAGCTCCTTGATGCAGCTTCTCAATTGGTCAAGCCTGGTGGAGCTTTAGTATACAGTACTTGCTCCATAGATCCGGAAGAGAATGAAGAGAGAATTGCTGCATTTCTTCTCAGGCATCCATATTTTTGCATAGATCCTGCTGACAAATATGTTCCATCTGAATTTGTAACGGAGCATGGTTTCTATTCCTCCAACCCTGTGAAACATTCCCTGGATGGGGCCTTTGCTGCTCGTTTAAGTCGAGCTTTGTAA

Protein Analysis

361

Amino Acids

39.94

Weight (kDa)

6.22

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RsmF-B_ferredox PF22458 64 - 136 9.5e-09 Methyltr_RsmF/B-like, ferredoxin-like domain
Methyltr_RsmB-F PF01189 163 - 357 1.8e-52 16S rRNA methyltransferase RsmB/F
TRM5-TYW2_MTfase PF02475 167 - 251 6.5e-07 TRM5/TYW2 methyltransferase domain
FtsJ PF01728 168 - 303 3e-08 FtsJ-like methyltransferase
Methyltransf_31 PF13847 168 - 301 8.7e-06 Methyltransferase domain
Methyltransf_15 PF09445 170 - 254 6.9e-06 RNA cap guanine-N2 methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 461
AccB7I CCANNNNNTGG 2 cut(s) 583, 749
AccI GTMKAC 1 cut(s) 885
AccIII TCCGGA 2 cut(s) 90, 907
AciI CCGC 2 cut(s) 497, 613
AclWI GGATC 4 cut(s) 266, 488, 899, 962
AcoI YGGCCR 1 cut(s) 574
AcsI RAATTY 2 cut(s) 823, 995
AcvI CACGTG 1 cut(s) 167
AcyI GRCGYC 1 cut(s) 560
AfaI GTAC 3 cut(s) 185, 456, 892
AfiI CCNNNNNNNGG 5 cut(s) 503, 583, 749, 1050, 1051
AflII CTTAAG 3 cut(s) 107, 286, 818
AgsI TTSAA 3 cut(s) 315, 379, 422
AjnI CCWGG 3 cut(s) 535, 868, 1044
AloI GAACNNNNNNTCC 2 cut(s) 744, 776
Alw21I GWGCWC 3 cut(s) 172, 366, 840
AlwI GGATC 4 cut(s) 266, 488, 899, 962
Aor13HI TCCGGA 2 cut(s) 90, 907
AoxI GGCC 2 cut(s) 574, 1053
ApeKI GCWGC 4 cut(s) 530, 848, 932, 1061
ApoI RAATTY 2 cut(s) 823, 995
AspS9I GGNCC 2 cut(s) 352, 1053
AsuHPI GGTGA 3 cut(s) 158, 518, 658
AvaII GGWCC 1 cut(s) 352
BalI TGGCCA 1 cut(s) 576
BanII GRGCYC 2 cut(s) 366, 840
BbrPI CACGTG 1 cut(s) 167
Bbv12I GWGCWC 3 cut(s) 172, 366, 840
BbvI GCAGC 4 cut(s) 517, 860, 919, 1048
BccI CCATC 5 cut(s) 207, 247, 650, 997, 1043
BceAI ACGGC 1 cut(s) 651
BciT130I CCWGG 3 cut(s) 537, 870, 1046
BfaI CTAG 1 cut(s) 276
BfrI CTTAAG 3 cut(s) 107, 286, 818
BfuAI ACCTGC 1 cut(s) 461
BisI GCNGC 4 cut(s) 531, 849, 933, 1062
BlsI GCNGC 4 cut(s) 532, 850, 934, 1063
BmcAI AGTACT 1 cut(s) 892
Bme1390I CCNGG 3 cut(s) 537, 870, 1046
Bme18I GGWCC 1 cut(s) 352
BmgT120I GGNCC 2 cut(s) 352, 1053
BmiI GGNNCC 3 cut(s) 354, 791, 1054
BmrFI CCNGG 3 cut(s) 537, 870, 1046
BmrI ACTGGG 1 cut(s) 764
BmsI GCATC 4 cut(s) 232, 727, 835, 958
BmuI ACTGGG 1 cut(s) 764
BpmI CTGGAG 1 cut(s) 558
BsaAI YACGTR 1 cut(s) 167
BsaHI GRCGYC 1 cut(s) 560
BsaJI CCNNGG 3 cut(s) 134, 577, 1044
BsaWI WCCGGW 3 cut(s) 90, 792, 907
BsaXI ACNNNNNCTCC 2 cut(s) 867, 897
Bsc4I CCNNNNNNNGG 5 cut(s) 503, 583, 749, 1050, 1051
Bse1I ACTGG 1 cut(s) 759
BseAI TCCGGA 2 cut(s) 90, 907
BseBI CCWGG 3 cut(s) 537, 870, 1046
BseDI CCNNGG 3 cut(s) 134, 577, 1044
BseGI GGATG 7 cut(s) 28, 190, 207, 742, 838, 949, 1054
BseLI CCNNNNNNNGG 5 cut(s) 503, 583, 749, 1050, 1051
BseMII CTCAG 1 cut(s) 958
BseNI ACTGG 1 cut(s) 759
BseRI GAGGAG 1 cut(s) 827
BseXI GCAGC 4 cut(s) 517, 860, 919, 1048
BsgI GTGCAG 1 cut(s) 489
BshFI GGCC 2 cut(s) 576, 1055
BsiHKAI GWGCWC 3 cut(s) 172, 366, 840
BsiSI CCGG 3 cut(s) 91, 793, 908
BslFI GGGAC 3 cut(s) 243, 338, 547
BslI CCNNNNNNNGG 5 cut(s) 503, 583, 749, 1050, 1051
BsmFI GGGAC 3 cut(s) 243, 338, 547
BsnI GGCC 2 cut(s) 576, 1055
Bsp1286I GDGCHC 3 cut(s) 172, 366, 840
Bsp13I TCCGGA 2 cut(s) 90, 907
Bsp1407I TGTACA 1 cut(s) 454
Bsp143I GATC 5 cut(s) 151, 271, 493, 904, 967
BspACI CCGC 2 cut(s) 497, 613
BspANI GGCC 2 cut(s) 576, 1055
BspCNI CTCAG 1 cut(s) 957
BspEI TCCGGA 2 cut(s) 90, 907
BspLI GGNNCC 3 cut(s) 354, 791, 1054
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 4 cut(s) 266, 488, 899, 962
BspTI CTTAAG 3 cut(s) 107, 286, 818
BsrGI TGTACA 1 cut(s) 454
BsrI ACTGG 1 cut(s) 759
BssECI CCNNGG 3 cut(s) 134, 577, 1044
BssMI GATC 5 cut(s) 151, 271, 493, 904, 967
BssNAI GTATAC 1 cut(s) 886
BssNI GRCGYC 1 cut(s) 560
BssT1I CCWWGG 2 cut(s) 134, 577
Bst1107I GTATAC 1 cut(s) 886
Bst2UI CCWGG 3 cut(s) 537, 870, 1046
Bst4CI ACNGT 3 cut(s) 9, 299, 890
Bst6I CTCTTC 3 cut(s) 129, 906, 915
BstACI GRCGYC 1 cut(s) 560
BstAFI CTTAAG 3 cut(s) 107, 286, 818
BstAUI TGTACA 1 cut(s) 454
BstBAI YACGTR 1 cut(s) 167
BstC8I GCNNGC 2 cut(s) 39, 67
BstDEI CTNAG 3 cut(s) 53, 639, 944
BstF5I GGATG 7 cut(s) 28, 190, 207, 742, 838, 949, 1054
BstKTI GATC 5 cut(s) 154, 274, 496, 907, 970
BstMBI GATC 5 cut(s) 151, 271, 493, 904, 967
BstMWI GCNNNNNNNGC 4 cut(s) 47, 370, 835, 1061
BstNI CCWGG 3 cut(s) 537, 870, 1046
BstSCI CCNGG 3 cut(s) 535, 868, 1044
BstV1I GCAGC 4 cut(s) 517, 860, 919, 1048
BstX2I RGATCY 3 cut(s) 493, 904, 967
BstXI CCANNNNNNTGG 1 cut(s) 656
BstYI RGATCY 3 cut(s) 493, 904, 967
BstZ17I GTATAC 1 cut(s) 886
BsuRI GGCC 2 cut(s) 576, 1055
BtgZI GCGATG 1 cut(s) 226
BtsCI GGATG 7 cut(s) 28, 190, 207, 742, 838, 949, 1054
BtsIMutI CAGTG 1 cut(s) 304
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 2 cut(s) 39, 67
Cfr13I GGNCC 2 cut(s) 352, 1053
CseI GACGC 1 cut(s) 549
Csp6I GTAC 3 cut(s) 184, 455, 891
CspCI CAANNNNNGTGG 2 cut(s) 853, 888
CviAII CATG 8 cut(s) 59, 73, 258, 359, 556, 677, 744, 1010
CviQI GTAC 3 cut(s) 184, 455, 891
DdeI CTNAG 3 cut(s) 53, 639, 944
DpnI GATC 5 cut(s) 153, 273, 495, 906, 969
DpnII GATC 5 cut(s) 151, 271, 493, 904, 967
EaeI YGGCCR 1 cut(s) 574
Eam1104I CTCTTC 3 cut(s) 129, 906, 915
EarI CTCTTC 3 cut(s) 129, 906, 915
Ecl136II GAGCTC 2 cut(s) 364, 838
Eco130I CCWWGG 2 cut(s) 134, 577
Eco24I GRGCYC 2 cut(s) 366, 840
Eco47I GGWCC 1 cut(s) 352
Eco53kI GAGCTC 2 cut(s) 364, 838
Eco72I CACGTG 1 cut(s) 167
EcoICRI GAGCTC 2 cut(s) 364, 838
EcoO109I RGGNCCY 2 cut(s) 352, 1053
EcoRII CCWGG 3 cut(s) 535, 868, 1044
EcoT14I CCWWGG 2 cut(s) 134, 577
EcoT38I GRGCYC 2 cut(s) 366, 840
ErhI CCWWGG 2 cut(s) 134, 577
FaeI CATG 8 cut(s) 62, 76, 261, 362, 559, 680, 747, 1013
FalI AAGNNNNNCTT 1 cut(s) 1063
FaqI GGGAC 3 cut(s) 243, 338, 547
FatI CATG 8 cut(s) 58, 72, 257, 358, 555, 676, 743, 1009
FblI GTMKAC 1 cut(s) 885
Fnu4HI GCNGC 4 cut(s) 531, 849, 933, 1062
FokI GGATG 7 cut(s) 35, 177, 214, 749, 845, 936, 1061
FriOI GRGCYC 2 cut(s) 366, 840
Fsp4HI GCNGC 4 cut(s) 531, 849, 933, 1062
FspBI CTAG 1 cut(s) 276
GluI GCNGC 4 cut(s) 531, 849, 933, 1062
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 2 cut(s) 576, 1055
HapII CCGG 3 cut(s) 91, 793, 908
HgaI GACGC 1 cut(s) 549
Hin1I GRCGYC 1 cut(s) 560
Hin1II CATG 8 cut(s) 62, 76, 261, 362, 559, 680, 747, 1013
HindIII AAGCTT 2 cut(s) 95, 335
HinfI GANTC 3 cut(s) 85, 547, 621
HpaII CCGG 3 cut(s) 91, 793, 908
HphI GGTGA 3 cut(s) 158, 518, 658
Hpy166II GTNNAC 1 cut(s) 886
Hpy188I TCNGA 1 cut(s) 994
Hpy188III TCNNGA 4 cut(s) 91, 315, 750, 908
Hpy8I GTNNAC 1 cut(s) 886
HpyAV CCTTC 2 cut(s) 434, 602
HpyCH4III ACNGT 3 cut(s) 9, 299, 890
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 9 cut(s) 373, 409, 470, 593, 646, 829, 848, 935, 963
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 370, 835, 1061
HpyF3I CTNAG 3 cut(s) 53, 639, 944
HpySE526I ACGT 1 cut(s) 166
Hsp92I GRCGYC 1 cut(s) 560
Hsp92II CATG 8 cut(s) 62, 76, 261, 362, 559, 680, 747, 1013
Kpn2I TCCGGA 2 cut(s) 90, 907
Kzo9I GATC 5 cut(s) 151, 271, 493, 904, 967
LmnI GCTCC 8 cut(s) 62, 538, 745, 814, 843, 875, 902, 1006
Lsp1109I GCAGC 4 cut(s) 517, 860, 919, 1048
LweI GCATC 4 cut(s) 232, 727, 835, 958
MaeI CTAG 1 cut(s) 276
MaeII ACGT 1 cut(s) 166
MaeIII GTNAC 4 cut(s) 116, 328, 664, 1000
MalI GATC 5 cut(s) 153, 273, 495, 906, 969
MboI GATC 5 cut(s) 151, 271, 493, 904, 967
MboII GAAGA 6 cut(s) 55, 116, 248, 923, 932, 932
MfeI CAATTG 2 cut(s) 594, 857
MflI RGATCY 3 cut(s) 493, 904, 967
MhlI GDGCHC 3 cut(s) 172, 366, 840
MlsI TGGCCA 1 cut(s) 576
MluCI AATT 8 cut(s) 243, 340, 417, 594, 823, 857, 927, 995
MluNI TGGCCA 1 cut(s) 576
MlyI GAGTC 1 cut(s) 541
MmeI TCCRAC 3 cut(s) 714, 767, 1050
MnlI CCTC 9 cut(s) 27, 136, 138, 343, 533, 765, 796, 805, 1033
Mox20I TGGCCA 1 cut(s) 576
MroI TCCGGA 2 cut(s) 90, 907
MscI TGGCCA 1 cut(s) 576
MseI TTAA 6 cut(s) 108, 287, 339, 627, 819, 1070
MslI CAYNNNNRTG 1 cut(s) 378
Msp20I TGGCCA 1 cut(s) 576
MspCI CTTAAG 3 cut(s) 107, 286, 818
MspI CCGG 3 cut(s) 91, 793, 908
MspR9I CCNGG 3 cut(s) 537, 870, 1046
MunI CAATTG 2 cut(s) 594, 857
MvaI CCWGG 3 cut(s) 537, 870, 1046
MwoI GCNNNNNNNGC 4 cut(s) 47, 370, 835, 1061
NdeII GATC 5 cut(s) 151, 271, 493, 904, 967
NlaIII CATG 8 cut(s) 62, 76, 261, 362, 559, 680, 747, 1013
NlaIV GGNNCC 3 cut(s) 354, 791, 1054
NmuCI GTSAC 2 cut(s) 328, 664
PcsI WCGNNNNNNNCGW 1 cut(s) 1072
PfeI GAWTC 2 cut(s) 85, 621
PflMI CCANNNNNTGG 2 cut(s) 583, 749
PfoI TCCNGGA 1 cut(s) 535
PkrI GCNGC 4 cut(s) 532, 850, 934, 1063
PleI GAGTC 1 cut(s) 541
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
PpsI GAGTC 1 cut(s) 541
Ppu21I YACGTR 1 cut(s) 167
PpuMI RGGWCCY 1 cut(s) 352
Psp124BI GAGCTC 2 cut(s) 366, 840
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 3 cut(s) 535, 868, 1044
PspCI CACGTG 1 cut(s) 167
PspGI CCWGG 3 cut(s) 535, 868, 1044
PspN4I GGNNCC 3 cut(s) 354, 791, 1054
PspPI GGNCC 2 cut(s) 352, 1053
PspPPI RGGWCCY 1 cut(s) 352
PsuI RGATCY 3 cut(s) 493, 904, 967
RsaI GTAC 3 cut(s) 185, 456, 892
RsaNI GTAC 3 cut(s) 184, 455, 891
RseI CAYNNNNRTG 1 cut(s) 378
SacI GAGCTC 2 cut(s) 366, 840
SaqAI TTAA 6 cut(s) 108, 287, 339, 627, 819, 1070
SatI GCNGC 4 cut(s) 531, 849, 933, 1062
Sau3AI GATC 5 cut(s) 151, 271, 493, 904, 967
Sau96I GGNCC 2 cut(s) 352, 1053
ScaI AGTACT 1 cut(s) 892
SchI GAGTC 1 cut(s) 541
ScrFI CCNGG 3 cut(s) 537, 870, 1046
SduI GDGCHC 3 cut(s) 172, 366, 840
SfaNI GCATC 4 cut(s) 232, 727, 835, 958
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 3 cut(s) 107, 286, 818
SmoI CTYRAG 3 cut(s) 107, 286, 818
Sse9I AATT 8 cut(s) 243, 340, 417, 594, 823, 857, 927, 995
SsiI CCGC 2 cut(s) 497, 613
SspMI CTAG 1 cut(s) 276
SstI GAGCTC 2 cut(s) 366, 840
StyD4I CCNGG 3 cut(s) 535, 868, 1044
StyI CCWWGG 2 cut(s) 134, 577
TaaI ACNGT 3 cut(s) 9, 299, 890
TaiI ACGT 1 cut(s) 169
TaqI TCGA 1 cut(s) 1075
TasI AATT 8 cut(s) 243, 340, 417, 594, 823, 857, 927, 995
TatI WGTACW 3 cut(s) 183, 454, 890
TfiI GAWTC 2 cut(s) 85, 621
Tru1I TTAA 6 cut(s) 108, 287, 339, 627, 819, 1070
Tru9I TTAA 6 cut(s) 108, 287, 339, 627, 819, 1070
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 2 cut(s) 328, 664
TseI GCWGC 4 cut(s) 530, 848, 932, 1061
Tsp45I GTSAC 2 cut(s) 328, 664
TspDTI ATGAA 4 cut(s) 410, 621, 728, 933
TspGWI ACGGA 2 cut(s) 380, 1019
TspRI CASTG 1 cut(s) 304
Van91I CCANNNNNTGG 2 cut(s) 583, 749
Vha464I CTTAAG 3 cut(s) 107, 286, 818
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 2 cut(s) 823, 995
XmiI GTMKAC 1 cut(s) 885
XspI CTAG 1 cut(s) 276
ZrmI AGTACT 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.