Rh5AG504900
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB NOP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010493
Physical Location & Seq
Forward (+)
85476526 .. 85482194
5669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG504900.1

Sequence Viewer

Length: 1419 bp
ATGTCACAGGTCCTCTCTTTCCATGTTCTTCTATCTGCAGAGACCCACAGGCCTTCTTCTTCTTCCTCCAAGTCACTCAAGTTGAAGCAGAGAACAAAGACATCCATTTCTACTCCCAGAAAAGGTAATTTCAATCCCTCACGCAAAACCCAGAAGCTGAATACCGAGGTTTCTACCTACAGAGGTGGTCAGTTCAATCCGGCACGCAAAACCCAGAAGCTGAATTTGGAGATTTCTCCTCACAGAGCTGTTTCTGCGGTGAGATTGATGAGAATAGAGATGGGAGGTGCTTTTGCTGATCTTCTCAATGAGAAAGGGAAGGGCTCTGGTGAAAATGAGATGGGATATGTTGAAAGAACTCTCGGGTTTCGCACACGGGATCTAGAGGATCGTGATCTTAGACTGGTCACTGAAATTGTTGGAGGCACCATACGTTGGAAGCGATATCTTGATCATTTGATCAGTTCGATATGTCGTGATGCAAACACATTTAGCAGGATGGAACCACTTCTTTTACAGATTCTCCGAATTGGTTTCTACGAGATTGTCAAGCTTAATATGCCACCGTATGCTGTTGTGGATGAGAATGTGAGGCTTGCGAAGATTGCCCTTAGACATGGTGCTGGCAACATGGTCAATGGGATTCTCCGGAAGCTTGTTCTGCTTAAGGAAAGTAACTCTCTTCCTCTACCCAAAGTAGAGGGTGATGATCGCACACAAGCACGTGCTCTTGCCACTTTGTACTCTCATCCTGTATGGATGATAAGGCGATGGACAAAGCATCTGGGACAAGAAGAAGCAATTAGATTGATGGCATGGAATAATAGTGATCCTAGTTTCAGCTTAAGGGCAAACAGTGGGAAAGGTATTTCAAGAGCTGAGCTTGTGACAAAGCTTAATTTATTGGAGGTCCCACATGAGCTCTCTTTGCATTTGAATGATTTTGTCCGTATGAAAAAAGGGTTGCAGATTGTAATTCAAGCTGGTTTGCTAAAAGAAGGTTTATGTTCTGTACAGGACGAGAGTGCAGGTCTGGTAGTTTCTGTTGTAGATCCGCAACAGGGTGAAAGCATTATTGATTGTTGTGCTGCTCCTGGAGGAAAGACTCTTTACATGGCGTCCCTTTTGAGTGGCCAAGGTATGGTATATGCAATTGACATAAATGAAGGGCGGTTGAGAATCCTCAAAGAAACGGCTAAGTTGCACCAAGTAGATGGTGTCGTAAACACTATCCATGCTGATATGCGTAGTTATGCTGACAATAATACTATGAAGTGTGATAAAGTTCTGTTGGATGCTCCATGTTCTGGACTGGGTGTTCTCTCCAAGAGGGCAGACTTGCGTTGGAACAGGAGATTAGAGGATATGGAGGAGCTTAAGAATTTGCAGGATGAGCTCCTTGATGCAGCTTCTCAGTAA

Protein Analysis

472

Amino Acids

52.76

Weight (kDa)

9.44

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NusB PF01029 111 - 219 3.3e-23 NusB family
RsmF-B_ferredox PF22458 250 - 322 1.4e-08 Methyltr_RsmF/B-like, ferredoxin-like domain
Methyltr_RsmB-F PF01189 349 - 470 4e-33 16S rRNA methyltransferase RsmB/F
TRM5-TYW2_MTfase PF02475 353 - 436 1e-06 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1019
AccB1I GGYRCC 1 cut(s) 425
AccB7I CCANNNNNTGG 3 cut(s) 435, 1141, 1307
AccIII TCCGGA 1 cut(s) 648
AciI CCGC 3 cut(s) 257, 1055, 1171
AclWI GGATC 4 cut(s) 387, 396, 824, 1046
AcoI YGGCCR 1 cut(s) 1132
AcsI RAATTY 2 cut(s) 223, 1381
AcvI CACGTG 1 cut(s) 725
AcyI GRCGYC 1 cut(s) 1118
AfaI GTAC 2 cut(s) 743, 1014
AfiI CCNNNNNNNGG 5 cut(s) 122, 435, 1061, 1141, 1307
AflII CTTAAG 3 cut(s) 665, 844, 1376
AgsI TTSAA 7 cut(s) 85, 133, 196, 353, 873, 937, 980
AjnI CCWGG 1 cut(s) 1093
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AloI GAACNNNNNNTCC 2 cut(s) 1302, 1334
Alw21I GWGCWC 3 cut(s) 730, 924, 1398
Alw26I GTCTC 1 cut(s) 35
AlwI GGATC 4 cut(s) 387, 396, 824, 1046
AlwNI CAGNNNCTG 2 cut(s) 157, 220
Ama87I CYCGRG 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 648
AoxI GGCC 2 cut(s) 50, 1132
ApeKI GCWGC 2 cut(s) 1088, 1406
ApoI RAATTY 2 cut(s) 223, 1381
Asp700I GAANNNNTTC 1 cut(s) 507
AspS9I GGNCC 2 cut(s) 10, 910
AsuHPI GGTGA 4 cut(s) 271, 341, 716, 1076
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 2 cut(s) 10, 910
BalI TGGCCA 1 cut(s) 1134
BanI GGYRCC 1 cut(s) 425
BanII GRGCYC 3 cut(s) 326, 924, 1398
BbrPI CACGTG 1 cut(s) 725
Bbv12I GWGCWC 3 cut(s) 730, 924, 1398
BbvI GCAGC 1 cut(s) 1075
BccI CCATC 6 cut(s) 274, 334, 493, 765, 805, 1208
BceAI ACGGC 1 cut(s) 1209
BciT130I CCWGG 1 cut(s) 1095
BclI TGATCA 2 cut(s) 451, 459
BcoDI GTCTC 1 cut(s) 35
BfaI CTAG 2 cut(s) 383, 834
BfmI CTRYAG 2 cut(s) 36, 178
BfrI CTTAAG 3 cut(s) 665, 844, 1376
BfuAI ACCTGC 1 cut(s) 1019
BisI GCNGC 2 cut(s) 1089, 1407
BlpI GCTNAGC 1 cut(s) 879
BlsI GCNGC 2 cut(s) 1090, 1408
Bme1390I CCNGG 1 cut(s) 1095
Bme18I GGWCC 2 cut(s) 10, 910
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 2 cut(s) 10, 910
BmiI GGNNCC 3 cut(s) 427, 504, 912
BmrFI CCNGG 1 cut(s) 1095
BmrI ACTGGG 1 cut(s) 1322
BmsI GCATC 4 cut(s) 469, 790, 1285, 1393
BmuI ACTGGG 1 cut(s) 1322
BpmI CTGGAG 1 cut(s) 1116
Bpu1102I GCTNAGC 1 cut(s) 879
BpuEI CTTGAG 1 cut(s) 62
BsaAI YACGTR 1 cut(s) 725
BsaBI GATNNNNATC 1 cut(s) 393
BsaHI GRCGYC 1 cut(s) 1118
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 165, 1135
BsaWI WCCGGW 1 cut(s) 648
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 5 cut(s) 122, 435, 1061, 1141, 1307
Bse1I ACTGG 2 cut(s) 408, 1317
Bse8I GATNNNNATC 1 cut(s) 393
BseAI TCCGGA 1 cut(s) 648
BseBI CCWGG 1 cut(s) 1095
BseDI CCNNGG 2 cut(s) 165, 1135
BseGI GGATG 7 cut(s) 101, 504, 586, 748, 765, 1300, 1396
BseJI GATNNNNATC 1 cut(s) 393
BseLI CCNNNNNNNGG 5 cut(s) 122, 435, 1061, 1141, 1307
BseMII CTCAG 1 cut(s) 870
BseNI ACTGG 2 cut(s) 408, 1317
BseRI GAGGAG 2 cut(s) 228, 1385
BseXI GCAGC 1 cut(s) 1075
BsgI GTGCAG 1 cut(s) 1047
BshFI GGCC 2 cut(s) 52, 1134
BshNI GGYRCC 1 cut(s) 425
BsiHKAI GWGCWC 3 cut(s) 730, 924, 1398
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 2 cut(s) 200, 649
BslFI GGGAC 3 cut(s) 801, 896, 1105
BslI CCNNNNNNNGG 5 cut(s) 122, 435, 1061, 1141, 1307
BsmAI GTCTC 1 cut(s) 35
BsmFI GGGAC 3 cut(s) 801, 896, 1105
BsnI GGCC 2 cut(s) 52, 1134
Bso31I GGTCTC 1 cut(s) 35
BsoBI CYCGRG 1 cut(s) 362
Bsp1286I GDGCHC 4 cut(s) 326, 730, 924, 1398
Bsp13I TCCGGA 1 cut(s) 648
Bsp1407I TGTACA 1 cut(s) 1012
Bsp143I GATC 9 cut(s) 298, 379, 388, 394, 451, 459, 709, 829, 1051
Bsp1720I GCTNAGC 1 cut(s) 879
BspACI CCGC 3 cut(s) 257, 1055, 1171
BspANI GGCC 2 cut(s) 52, 1134
BspCNI CTCAG 1 cut(s) 871
BspEI TCCGGA 1 cut(s) 648
BspLI GGNNCC 3 cut(s) 427, 504, 912
BspMAI CTGCAG 1 cut(s) 40
BspMI ACCTGC 1 cut(s) 1019
BspPI GGATC 4 cut(s) 387, 396, 824, 1046
BspT107I GGYRCC 1 cut(s) 425
BspTI CTTAAG 3 cut(s) 665, 844, 1376
BspTNI GGTCTC 1 cut(s) 35
BsrGI TGTACA 1 cut(s) 1012
BsrI ACTGG 2 cut(s) 408, 1317
BssECI CCNNGG 2 cut(s) 165, 1135
BssMI GATC 9 cut(s) 298, 379, 388, 394, 451, 459, 709, 829, 1051
BssNI GRCGYC 1 cut(s) 1118
BssT1I CCWWGG 1 cut(s) 1135
Bst2UI CCWGG 1 cut(s) 1095
Bst4CI ACNGT 2 cut(s) 567, 857
Bst6I CTCTTC 1 cut(s) 687
BstACI GRCGYC 1 cut(s) 1118
BstAFI CTTAAG 3 cut(s) 665, 844, 1376
BstAUI TGTACA 1 cut(s) 1012
BstBAI YACGTR 1 cut(s) 725
BstC8I GCNNGC 3 cut(s) 205, 597, 625
BstDEI CTNAG 5 cut(s) 398, 611, 879, 1197, 1413
BstF5I GGATG 7 cut(s) 101, 504, 586, 748, 765, 1300, 1396
BstKTI GATC 9 cut(s) 301, 382, 391, 397, 454, 462, 712, 832, 1054
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 9 cut(s) 298, 379, 388, 394, 451, 459, 709, 829, 1051
BstMWI GCNNNNNNNGC 6 cut(s) 254, 559, 605, 661, 928, 1393
BstNI CCWGG 1 cut(s) 1095
BstSCI CCNGG 1 cut(s) 1093
BstSFI CTRYAG 2 cut(s) 36, 178
BstV1I GCAGC 1 cut(s) 1075
BstX2I RGATCY 2 cut(s) 379, 1051
BstXI CCANNNNNNTGG 1 cut(s) 1214
BstYI RGATCY 2 cut(s) 379, 1051
BsuRI GGCC 2 cut(s) 52, 1134
BtgZI GCGATG 1 cut(s) 784
BtsCI GGATG 7 cut(s) 101, 504, 586, 748, 765, 1300, 1396
BtsIMutI CAGTG 2 cut(s) 408, 862
BveI ACCTGC 1 cut(s) 1019
Cac8I GCNNGC 3 cut(s) 205, 597, 625
CaiI CAGNNNCTG 2 cut(s) 157, 220
Cfr13I GGNCC 2 cut(s) 10, 910
CseI GACGC 1 cut(s) 1107
Csp6I GTAC 2 cut(s) 742, 1013
CviAII CATG 8 cut(s) 23, 617, 631, 816, 917, 1114, 1235, 1302
CviQI GTAC 2 cut(s) 742, 1013
DdeI CTNAG 5 cut(s) 398, 611, 879, 1197, 1413
DpnI GATC 9 cut(s) 300, 381, 390, 396, 453, 461, 711, 831, 1053
DpnII GATC 9 cut(s) 298, 379, 388, 394, 451, 459, 709, 829, 1051
EaeI YGGCCR 1 cut(s) 1132
Eam1104I CTCTTC 1 cut(s) 687
EarI CTCTTC 1 cut(s) 687
Ecl136II GAGCTC 2 cut(s) 922, 1396
Eco130I CCWWGG 1 cut(s) 1135
Eco147I AGGCCT 1 cut(s) 52
Eco24I GRGCYC 3 cut(s) 326, 924, 1398
Eco31I GGTCTC 1 cut(s) 35
Eco32I GATATC 1 cut(s) 446
Eco47I GGWCC 2 cut(s) 10, 910
Eco53kI GAGCTC 2 cut(s) 922, 1396
Eco72I CACGTG 1 cut(s) 725
Eco88I CYCGRG 1 cut(s) 362
EcoICRI GAGCTC 2 cut(s) 922, 1396
EcoO109I RGGNCCY 2 cut(s) 10, 910
EcoRII CCWGG 1 cut(s) 1093
EcoRV GATATC 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 1135
EcoT38I GRGCYC 3 cut(s) 326, 924, 1398
ErhI CCWWGG 1 cut(s) 1135
FaeI CATG 8 cut(s) 26, 620, 634, 819, 920, 1117, 1238, 1305
FaqI GGGAC 3 cut(s) 801, 896, 1105
FatI CATG 8 cut(s) 22, 616, 630, 815, 916, 1113, 1234, 1301
FbaI TGATCA 2 cut(s) 451, 459
Fnu4HI GCNGC 2 cut(s) 1089, 1407
FokI GGATG 7 cut(s) 88, 511, 593, 735, 772, 1307, 1403
FriOI GRGCYC 3 cut(s) 326, 924, 1398
Fsp4HI GCNGC 2 cut(s) 1089, 1407
FspBI CTAG 2 cut(s) 383, 834
GluI GCNGC 2 cut(s) 1089, 1407
GsuI CTGGAG 1 cut(s) 1116
HaeIII GGCC 2 cut(s) 52, 1134
HapII CCGG 2 cut(s) 200, 649
HgaI GACGC 1 cut(s) 1107
Hin1I GRCGYC 1 cut(s) 1118
Hin1II CATG 8 cut(s) 26, 620, 634, 819, 920, 1117, 1238, 1305
HindIII AAGCTT 3 cut(s) 551, 653, 893
HinfI GANTC 4 cut(s) 520, 643, 1105, 1179
HpaII CCGG 2 cut(s) 200, 649
HphI GGTGA 4 cut(s) 271, 341, 716, 1076
Hpy166II GTNNAC 1 cut(s) 1225
Hpy188I TCNGA 1 cut(s) 527
Hpy188III TCNNGA 7 cut(s) 383, 392, 449, 476, 649, 873, 1308
Hpy8I GTNNAC 1 cut(s) 1225
HpyAV CCTTC 4 cut(s) 63, 313, 992, 1160
HpyCH4III ACNGT 2 cut(s) 567, 857
HpyCH4IV ACGT 2 cut(s) 433, 724
HpyCH4V TGCA 9 cut(s) 38, 482, 931, 967, 1028, 1151, 1204, 1387, 1406
HpyF10VI GCNNNNNNNGC 6 cut(s) 254, 559, 605, 661, 928, 1393
HpyF3I CTNAG 5 cut(s) 398, 611, 879, 1197, 1413
HpySE526I ACGT 2 cut(s) 433, 724
Hsp92I GRCGYC 1 cut(s) 1118
Hsp92II CATG 8 cut(s) 26, 620, 634, 819, 920, 1117, 1238, 1305
Kpn2I TCCGGA 1 cut(s) 648
Ksp22I TGATCA 2 cut(s) 451, 459
Kzo9I GATC 9 cut(s) 298, 379, 388, 394, 451, 459, 709, 829, 1051
LmnI GCTCC 4 cut(s) 1096, 1303, 1372, 1401
Lsp1109I GCAGC 1 cut(s) 1075
LweI GCATC 4 cut(s) 469, 790, 1285, 1393
MaeI CTAG 2 cut(s) 383, 834
MaeII ACGT 2 cut(s) 433, 724
MaeIII GTNAC 5 cut(s) 3, 72, 406, 674, 886
MalI GATC 9 cut(s) 300, 381, 390, 396, 453, 461, 711, 831, 1053
MboI GATC 9 cut(s) 298, 379, 388, 394, 451, 459, 709, 829, 1051
MboII GAAGA 8 cut(s) 20, 48, 51, 54, 293, 613, 674, 806
MfeI CAATTG 1 cut(s) 1152
MflI RGATCY 2 cut(s) 379, 1051
MhlI GDGCHC 4 cut(s) 326, 730, 924, 1398
MlsI TGGCCA 1 cut(s) 1134
MluCI AATT 9 cut(s) 127, 223, 414, 528, 801, 898, 975, 1152, 1381
MluNI TGGCCA 1 cut(s) 1134
MlyI GAGTC 1 cut(s) 1099
MmeI TCCRAC 4 cut(s) 400, 416, 1272, 1325
Mox20I TGGCCA 1 cut(s) 1134
MroI TCCGGA 1 cut(s) 648
MroXI GAANNNNTTC 1 cut(s) 507
MscI TGGCCA 1 cut(s) 1134
MseI TTAA 5 cut(s) 555, 666, 845, 897, 1377
MslI CAYNNNNRTG 1 cut(s) 936
Msp20I TGGCCA 1 cut(s) 1134
MspCI CTTAAG 3 cut(s) 665, 844, 1376
MspI CCGG 2 cut(s) 200, 649
MspR9I CCNGG 1 cut(s) 1095
MunI CAATTG 1 cut(s) 1152
MvaI CCWGG 1 cut(s) 1095
MwoI GCNNNNNNNGC 6 cut(s) 254, 559, 605, 661, 928, 1393
NdeII GATC 9 cut(s) 298, 379, 388, 394, 451, 459, 709, 829, 1051
NlaIII CATG 8 cut(s) 26, 620, 634, 819, 920, 1117, 1238, 1305
NlaIV GGNNCC 3 cut(s) 427, 504, 912
NmuCI GTSAC 4 cut(s) 3, 72, 406, 886
PceI AGGCCT 1 cut(s) 52
PcsI WCGNNNNNNNCGW 1 cut(s) 439
PdmI GAANNNNTTC 1 cut(s) 507
PfeI GAWTC 3 cut(s) 520, 643, 1179
PflMI CCANNNNNTGG 3 cut(s) 435, 1141, 1307
PfoI TCCNGGA 1 cut(s) 1093
PkrI GCNGC 2 cut(s) 1090, 1408
PleI GAGTC 1 cut(s) 1099
PmaCI CACGTG 1 cut(s) 725
PmlI CACGTG 1 cut(s) 725
PpsI GAGTC 1 cut(s) 1099
Ppu21I YACGTR 1 cut(s) 725
PpuMI RGGWCCY 2 cut(s) 10, 910
Psp124BI GAGCTC 2 cut(s) 924, 1398
Psp5II RGGWCCY 2 cut(s) 10, 910
Psp6I CCWGG 1 cut(s) 1093
PspCI CACGTG 1 cut(s) 725
PspGI CCWGG 1 cut(s) 1093
PspN4I GGNNCC 3 cut(s) 427, 504, 912
PspPI GGNCC 2 cut(s) 10, 910
PspPPI RGGWCCY 2 cut(s) 10, 910
PstI CTGCAG 1 cut(s) 40
PstNI CAGNNNCTG 2 cut(s) 157, 220
PsuI RGATCY 2 cut(s) 379, 1051
RsaI GTAC 2 cut(s) 743, 1014
RsaNI GTAC 2 cut(s) 742, 1013
RseI CAYNNNNRTG 1 cut(s) 936
SacI GAGCTC 2 cut(s) 924, 1398
SaqAI TTAA 5 cut(s) 555, 666, 845, 897, 1377
SatI GCNGC 2 cut(s) 1089, 1407
Sau3AI GATC 9 cut(s) 298, 379, 388, 394, 451, 459, 709, 829, 1051
Sau96I GGNCC 2 cut(s) 10, 910
SchI GAGTC 1 cut(s) 1099
ScrFI CCNGG 1 cut(s) 1095
SduI GDGCHC 4 cut(s) 326, 730, 924, 1398
SfaNI GCATC 4 cut(s) 469, 790, 1285, 1393
SfcI CTRYAG 2 cut(s) 36, 178
SinI GGWCC 2 cut(s) 10, 910
SmiMI CAYNNNNRTG 1 cut(s) 936
SmlI CTYRAG 4 cut(s) 77, 665, 844, 1376
SmoI CTYRAG 4 cut(s) 77, 665, 844, 1376
Sse9I AATT 9 cut(s) 127, 223, 414, 528, 801, 898, 975, 1152, 1381
SseBI AGGCCT 1 cut(s) 52
SsiI CCGC 3 cut(s) 257, 1055, 1171
SspMI CTAG 2 cut(s) 383, 834
SstI GAGCTC 2 cut(s) 924, 1398
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 1 cut(s) 1093
StyI CCWWGG 1 cut(s) 1135
TaaI ACNGT 2 cut(s) 567, 857
TaiI ACGT 2 cut(s) 436, 727
TaqI TCGA 1 cut(s) 467
TasI AATT 9 cut(s) 127, 223, 414, 528, 801, 898, 975, 1152, 1381
TatI WGTACW 2 cut(s) 741, 1012
TfiI GAWTC 3 cut(s) 520, 643, 1179
Tru1I TTAA 5 cut(s) 555, 666, 845, 897, 1377
Tru9I TTAA 5 cut(s) 555, 666, 845, 897, 1377
TscAI CASTG 2 cut(s) 415, 862
TseFI GTSAC 4 cut(s) 3, 72, 406, 886
TseI GCWGC 2 cut(s) 1088, 1406
Tsp45I GTSAC 4 cut(s) 3, 72, 406, 886
TspDTI ATGAA 3 cut(s) 968, 1179, 1286
TspGWI ACGGA 1 cut(s) 938
TspRI CASTG 2 cut(s) 415, 862
Van91I CCANNNNNTGG 3 cut(s) 435, 1141, 1307
Vha464I CTTAAG 3 cut(s) 665, 844, 1376
VpaK11BI GGWCC 2 cut(s) 10, 910
XapI RAATTY 2 cut(s) 223, 1381
XbaI TCTAGA 1 cut(s) 382
XmnI GAANNNNTTC 1 cut(s) 507
XspI CTAG 2 cut(s) 383, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.