Rroxscaffold_1G00043100

TBC1 domain family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007138
Physical Location & Seq
Forward (+)
61409766 .. 61416808
7043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00043100.1

Sequence Viewer

Length: 753 bp
ATGTGGGGGAGCTCCGGAACTCCTGCTGATTCTTTCTATGAGGTCCGCCCCGAGTGTACTGATGTACCCAAAACCCGGTTCAAGATCAAGGCAGGTAAGACTCTAAGTTCAAGGAAATGGCAGGCCGCATTTTCTCCAGAAGGTTATCTGGATATAGGCAAGACTTTGAGTCGAATCCATCGCGGGGGTATCCATCCATCAATTAGAGGAGAAGTTTGGGAATTTCTACTTGGTTGTTATCACCCGAATAGTACATTTGAAGAAAGAGAGCAGCTCCGGCAACGTCGAAGGATAGACTATGCTAGGTGGAAGGAAGATTGCCGCCAAATGTTTCCTGTTGTTGGAAGTGGTAGGTTCATCACTGCCCCTGTAATCACTGATGATGGCCAGCCCATTCAGGATCCAATAGTACTTATGCAAATAAATCCAGATAAGGGACAGGCTTTATCTTCACAGGAAAATGGTAAAGCCAATGGTAATACCATGAATCCTGCTAGTGCTTTGGAACCAGTGAAGGATAAGAAAATAATCCAGTGGCTGCGTACTTTGCATCAAATCGGGCTTGACGTAATTCGCACTGACAGGACGCTAGTGTTTTACGAGAAGCAAGAAAACTTATCAAAACTTTGGGATATTCTAGCTGTTTATGCCTGGATAGATACAGATGTCGGCTACTGTCAAGGAATGAGTGATCTTTGCTCCCCCATGATTATCCTTCTTGATGATGAAAGCAGATGCATTTTGGTGTTTTGA

Protein Analysis

250

Amino Acids

28.54

Weight (kDa)

6.9

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RabGAP-TBC PF00566 171 - 246 6.9e-15 Rab-GTPase-TBC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 83
AccII CGCG 1 cut(s) 183
AccIII TCCGGA 1 cut(s) 14
AciI CCGC 4 cut(s) 46, 126, 183, 322
AclWI GGATC 2 cut(s) 395, 408
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 1 cut(s) 221
AfaI GTAC 5 cut(s) 58, 66, 253, 411, 544
AfiI CCNNNNNNNGG 4 cut(s) 75, 184, 341, 434
AgsI TTSAA 3 cut(s) 82, 111, 260
AhdI GACNNNNNGTC 1 cut(s) 168
AjnI CCWGG 1 cut(s) 650
AjuI GAANNNNNNNTTGG 4 cut(s) 62, 94, 213, 245
AluBI AGCT 3 cut(s) 12, 274, 641
AluI AGCT 3 cut(s) 12, 274, 641
Alw21I GWGCWC 1 cut(s) 14
AlwI GGATC 2 cut(s) 395, 408
AlwNI CAGNNNCTG 1 cut(s) 538
Ama87I CYCGRG 1 cut(s) 50
Aor13HI TCCGGA 1 cut(s) 14
AoxI GGCC 2 cut(s) 123, 385
ApeKI GCWGC 2 cut(s) 271, 538
ApoI RAATTY 1 cut(s) 221
AspS9I GGNCC 1 cut(s) 43
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 1 cut(s) 233
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 1 cut(s) 43
BaeI ACNNNNGTAYC 4 cut(s) 48, 81, 651, 684
BalI TGGCCA 1 cut(s) 387
BamHI GGATCC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 14
BbvI GCAGC 2 cut(s) 283, 525
BccI CCATC 4 cut(s) 186, 201, 205, 377
BciT130I CCWGG 1 cut(s) 652
BciVI GTATCC 1 cut(s) 200
BcnI CCSGG 1 cut(s) 76
BfaI CTAG 4 cut(s) 303, 495, 590, 638
BfuAI ACCTGC 1 cut(s) 83
BfuI GTATCC 1 cut(s) 200
BisI GCNGC 4 cut(s) 126, 272, 322, 539
BlsI GCNGC 4 cut(s) 127, 273, 323, 540
BmcAI AGTACT 1 cut(s) 411
Bme1390I CCNGG 2 cut(s) 76, 652
Bme18I GGWCC 1 cut(s) 43
BmeRI GACNNNNNGTC 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 43
BmiI GGNNCC 2 cut(s) 402, 507
BmrFI CCNGG 2 cut(s) 76, 652
BmsI GCATC 2 cut(s) 559, 725
BplI GAGNNNNNCTC 2 cut(s) 258, 290
BpmI CTGGAG 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 76
BsaWI WCCGGW 1 cut(s) 14
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 184, 341, 434
Bse1I ACTGG 2 cut(s) 509, 532
BseAI TCCGGA 1 cut(s) 14
BseBI CCWGG 1 cut(s) 652
BseGI GGATG 1 cut(s) 193
BseLI CCNNNNNNNGG 4 cut(s) 75, 184, 341, 434
BseNI ACTGG 2 cut(s) 509, 532
BseRI GAGGAG 1 cut(s) 222
BseXI GCAGC 2 cut(s) 283, 525
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 2 cut(s) 125, 387
BsiHKAI GWGCWC 1 cut(s) 14
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 3 cut(s) 15, 76, 277
BslFI GGGAC 1 cut(s) 450
BslI CCNNNNNNNGG 4 cut(s) 75, 184, 341, 434
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 2 cut(s) 125, 387
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 14
Bsp13I TCCGGA 1 cut(s) 14
Bsp143I GATC 3 cut(s) 84, 400, 691
BspACI CCGC 4 cut(s) 46, 126, 183, 322
BspANI GGCC 2 cut(s) 125, 387
BspEI TCCGGA 1 cut(s) 14
BspFNI CGCG 1 cut(s) 183
BspLI GGNNCC 2 cut(s) 402, 507
BspMI ACCTGC 1 cut(s) 83
BspPI GGATC 2 cut(s) 395, 408
BsrI ACTGG 2 cut(s) 509, 532
BssMI GATC 3 cut(s) 84, 400, 691
Bst2UI CCWGG 1 cut(s) 652
Bst4CI ACNGT 1 cut(s) 677
BstC8I GCNNGC 2 cut(s) 123, 389
BstDEI CTNAG 1 cut(s) 104
BstF5I GGATG 1 cut(s) 193
BstFNI CGCG 1 cut(s) 183
BstKTI GATC 3 cut(s) 87, 403, 694
BstMBI GATC 3 cut(s) 84, 400, 691
BstMWI GCNNNNNNNGC 3 cut(s) 277, 547, 647
BstNI CCWGG 1 cut(s) 652
BstSCI CCNGG 2 cut(s) 74, 650
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 283, 525
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
BsuI GTATCC 1 cut(s) 200
BsuRI GGCC 2 cut(s) 125, 387
BtgZI GCGATG 1 cut(s) 164
BtsCI GGATG 1 cut(s) 193
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 5 cut(s) 360, 375, 516, 539, 576
BveI ACCTGC 1 cut(s) 83
Cac8I GCNNGC 2 cut(s) 123, 389
CaiI CAGNNNCTG 1 cut(s) 538
Cfr13I GGNCC 1 cut(s) 43
CseI GACGC 1 cut(s) 595
Csp6I GTAC 5 cut(s) 57, 65, 252, 410, 543
CviAII CATG 2 cut(s) 484, 706
CviQI GTAC 5 cut(s) 57, 65, 252, 410, 543
DdeI CTNAG 1 cut(s) 104
DpnI GATC 3 cut(s) 86, 402, 693
DpnII GATC 3 cut(s) 84, 400, 691
DriI GACNNNNNGTC 1 cut(s) 168
EaeI YGGCCR 1 cut(s) 385
Eam1105I GACNNNNNGTC 1 cut(s) 168
EciI GGCGGA 1 cut(s) 35
Ecl136II GAGCTC 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 43
Eco53kI GAGCTC 1 cut(s) 12
Eco88I CYCGRG 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 12
EcoRII CCWGG 1 cut(s) 650
EcoT22I ATGCAT 1 cut(s) 740
EcoT38I GRGCYC 1 cut(s) 14
FaeI CATG 2 cut(s) 487, 709
FaiI YATR 7 cut(s) 39, 155, 300, 416, 485, 648, 707
FaqI GGGAC 1 cut(s) 450
FatI CATG 2 cut(s) 483, 705
FauI CCCGC 1 cut(s) 176
Fnu4HI GCNGC 4 cut(s) 126, 272, 322, 539
FokI GGATG 1 cut(s) 180
FriOI GRGCYC 1 cut(s) 14
Fsp4HI GCNGC 4 cut(s) 126, 272, 322, 539
FspBI CTAG 4 cut(s) 303, 495, 590, 638
GluI GCNGC 4 cut(s) 126, 272, 322, 539
GsuI CTGGAG 1 cut(s) 120
HaeIII GGCC 2 cut(s) 125, 387
HapII CCGG 3 cut(s) 15, 76, 277
HgaI GACGC 1 cut(s) 595
Hin1II CATG 2 cut(s) 487, 709
HinfI GANTC 5 cut(s) 29, 100, 169, 174, 487
HpaII CCGG 3 cut(s) 15, 76, 277
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 57
Hpy188III TCNNGA 7 cut(s) 15, 82, 137, 149, 398, 428, 719
Hpy8I GTNNAC 1 cut(s) 57
Hpy99I CGWCG 1 cut(s) 288
HpyAV CCTTC 5 cut(s) 134, 282, 304, 508, 725
HpyCH4III ACNGT 1 cut(s) 677
HpyCH4IV ACGT 2 cut(s) 283, 567
HpyCH4V TGCA 3 cut(s) 418, 550, 738
HpyF10VI GCNNNNNNNGC 3 cut(s) 277, 547, 647
HpyF3I CTNAG 1 cut(s) 104
HpySE526I ACGT 2 cut(s) 283, 567
Hsp92II CATG 2 cut(s) 487, 709
Kpn2I TCCGGA 1 cut(s) 14
Kzo9I GATC 3 cut(s) 84, 400, 691
LmnI GCTCC 4 cut(s) 9, 17, 279, 704
Lsp1109I GCAGC 2 cut(s) 283, 525
LweI GCATC 2 cut(s) 559, 725
MaeI CTAG 4 cut(s) 303, 495, 590, 638
MaeII ACGT 2 cut(s) 283, 567
MalI GATC 3 cut(s) 86, 402, 693
MboI GATC 3 cut(s) 84, 400, 691
MboII GAAGA 3 cut(s) 272, 326, 441
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 1 cut(s) 14
MlsI TGGCCA 1 cut(s) 387
MluCI AATT 3 cut(s) 201, 221, 570
MluNI TGGCCA 1 cut(s) 387
MlyI GAGTC 2 cut(s) 94, 178
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 2 cut(s) 34, 200
Mox20I TGGCCA 1 cut(s) 387
Mph1103I ATGCAT 1 cut(s) 740
MroI TCCGGA 1 cut(s) 14
MscI TGGCCA 1 cut(s) 387
MslI CAYNNNNRTG 1 cut(s) 743
Msp20I TGGCCA 1 cut(s) 387
MspI CCGG 3 cut(s) 15, 76, 277
MspR9I CCNGG 2 cut(s) 76, 652
MvaI CCWGG 1 cut(s) 652
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 3 cut(s) 277, 547, 647
NciI CCSGG 1 cut(s) 76
NdeII GATC 3 cut(s) 84, 400, 691
NlaIII CATG 2 cut(s) 487, 709
NlaIV GGNNCC 2 cut(s) 402, 507
NsiI ATGCAT 1 cut(s) 740
PcsI WCGNNNNNNNCGW 1 cut(s) 564
PfeI GAWTC 3 cut(s) 29, 174, 487
PkrI GCNGC 4 cut(s) 127, 273, 323, 540
PleI GAGTC 2 cut(s) 94, 177
PpsI GAGTC 2 cut(s) 94, 177
Psp124BI GAGCTC 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 650
PspGI CCWGG 1 cut(s) 650
PspN4I GGNNCC 2 cut(s) 402, 507
PspPI GGNCC 1 cut(s) 43
PstNI CAGNNNCTG 1 cut(s) 538
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 5 cut(s) 58, 66, 253, 411, 544
RsaNI GTAC 5 cut(s) 57, 65, 252, 410, 543
RseI CAYNNNNRTG 1 cut(s) 743
SacI GAGCTC 1 cut(s) 14
SatI GCNGC 4 cut(s) 126, 272, 322, 539
Sau3AI GATC 3 cut(s) 84, 400, 691
Sau96I GGNCC 1 cut(s) 43
ScaI AGTACT 1 cut(s) 411
SchI GAGTC 2 cut(s) 94, 178
ScrFI CCNGG 2 cut(s) 76, 652
SduI GDGCHC 1 cut(s) 14
SfaNI GCATC 2 cut(s) 559, 725
SinI GGWCC 1 cut(s) 43
SmiMI CAYNNNNRTG 1 cut(s) 743
Sse9I AATT 3 cut(s) 201, 221, 570
SsiI CCGC 4 cut(s) 46, 126, 183, 322
SspMI CTAG 4 cut(s) 303, 495, 590, 638
SstI GAGCTC 1 cut(s) 14
StyD4I CCNGG 2 cut(s) 74, 650
TaaI ACNGT 1 cut(s) 677
TaiI ACGT 2 cut(s) 286, 570
TaqI TCGA 2 cut(s) 172, 286
TasI AATT 3 cut(s) 201, 221, 570
TatI WGTACW 3 cut(s) 56, 251, 409
TauI GCSGC 2 cut(s) 128, 324
TfiI GAWTC 3 cut(s) 29, 174, 487
TscAI CASTG 5 cut(s) 367, 382, 516, 539, 583
TseI GCWGC 2 cut(s) 271, 538
TspDTI ATGAA 3 cut(s) 346, 500, 741
TspRI CASTG 5 cut(s) 367, 382, 516, 539, 583
VpaK11BI GGWCC 1 cut(s) 43
XapI RAATTY 1 cut(s) 221
XspI CTAG 4 cut(s) 303, 495, 590, 638
ZrmI AGTACT 1 cut(s) 411
Zsp2I ATGCAT 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.