Rroxscaffold_1G00057360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016990
Physical Location & Seq
Forward (+)
79326872 .. 79330366
3495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00057360.1

Sequence Viewer

Length: 537 bp
ATGGAAGCATATCTTCTCTCTTCCTCTGAAGAGAAAGCCAGGTTGATCAGCACTATACGTAGGGATATGATAAACCTGCCACTTTTGCCTACTGAATTTCAAAGACCAACCAGTTACAAGTTGGTTGATCTTCGAGCTCAAAGAGCTTGCCTTGTTTTGAGCGCATTCAAGCGAACGACTTCCATGCTAGGTTATCCTAAGATGAACACTTCAGTGACTCTTGTTATACCCTTTAAGATTCTTTGCAGCGCTGATTGTACTTTGGGCCGACAAGCTCGTCTCCCCGACTTCTTCCTCCACCACACAACCGCCGACCTCAACCTCAACCTCAACCACCTTACGTCTTCGTCGCCGTCGGGTTCCCCAAGGAAGACCGGCCAAGATTCGGATCGAGAAACGGTCCGAGAGGGCCTAAAATCCGCCGGAAAAGGTTTCGTCACCGTCATCGACTCGCCGGCGACCACTTCGCAGCGAAGTCTTTACCGCCCGGATTGGATTCTTGTGCTCGGACGCTCCAACGAGGAGGTGGAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

19.87

Weight (kDa)

9.3

Isoelectric Point (pI)

50.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 276
Acc36I ACCTGC 1 cut(s) 84
AciI CCGC 3 cut(s) 309, 420, 484
AclWI GGATC 1 cut(s) 396
AcoI YGGCCR 1 cut(s) 376
AcsI RAATTY 1 cut(s) 95
AcuI CTGAAG 2 cut(s) 48, 195
AfaI GTAC 1 cut(s) 259
AfeI AGCGCT 1 cut(s) 250
AfiI CCNNNNNNNGG 1 cut(s) 385
AgsI TTSAA 2 cut(s) 101, 169
AjnI CCWGG 1 cut(s) 38
AleI CACNNNNGTG 1 cut(s) 212
AluBI AGCT 3 cut(s) 137, 146, 275
AluI AGCT 3 cut(s) 137, 146, 275
Alw21I GWGCWC 2 cut(s) 139, 507
Alw26I GTCTC 1 cut(s) 284
AlwI GGATC 1 cut(s) 396
Aor51HI AGCGCT 1 cut(s) 250
AoxI GGCC 3 cut(s) 265, 376, 409
ApeKI GCWGC 2 cut(s) 246, 469
ApoI RAATTY 1 cut(s) 95
Asp700I GAANNNNTTC 1 cut(s) 178
AspLEI GCGC 2 cut(s) 164, 251
AspS9I GGNCC 3 cut(s) 265, 400, 409
AsuC2I CCSGG 1 cut(s) 488
AsuHPI GGTGA 1 cut(s) 430
AvaII GGWCC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 139
BbsI GAAGAC 2 cut(s) 336, 377
Bbv12I GWGCWC 2 cut(s) 139, 507
BbvI GCAGC 2 cut(s) 258, 481
BceAI ACGGC 1 cut(s) 337
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 1 cut(s) 40
BclI TGATCA 1 cut(s) 45
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 1 cut(s) 284
BfaI CTAG 1 cut(s) 188
BfoI RGCGCY 1 cut(s) 252
BfuAI ACCTGC 1 cut(s) 84
BisI GCNGC 2 cut(s) 247, 470
BlsI GCNGC 2 cut(s) 248, 471
Bme1390I CCNGG 2 cut(s) 40, 488
Bme18I GGWCC 1 cut(s) 400
BmgT120I GGNCC 3 cut(s) 265, 400, 409
BmiI GGNNCC 1 cut(s) 361
BmrFI CCNGG 2 cut(s) 40, 488
BpiI GAAGAC 2 cut(s) 336, 377
BpuMI CCSGG 1 cut(s) 488
BsaAI YACGTR 1 cut(s) 59
BsaBI GATNNNNATC 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 365
Bsc4I CCNNNNNNNGG 1 cut(s) 385
Bse118I RCCGGY 2 cut(s) 374, 454
Bse1I ACTGG 1 cut(s) 111
Bse8I GATNNNNATC 1 cut(s) 387
BseBI CCWGG 1 cut(s) 40
BseDI CCNNGG 1 cut(s) 365
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 1 cut(s) 385
BseNI ACTGG 1 cut(s) 111
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 2 cut(s) 258, 481
BshFI GGCC 3 cut(s) 267, 378, 411
BsiHKAI GWGCWC 2 cut(s) 139, 507
BsiSI CCGG 4 cut(s) 375, 423, 455, 488
BslI CCNNNNNNNGG 1 cut(s) 385
BsmAI GTCTC 1 cut(s) 284
BsmBI CGTCTC 1 cut(s) 284
BsmI GAATGC 1 cut(s) 164
BsnI GGCC 3 cut(s) 267, 378, 411
Bsp1286I GDGCHC 2 cut(s) 139, 507
Bsp143I GATC 3 cut(s) 45, 127, 388
BspACI CCGC 3 cut(s) 309, 420, 484
BspANI GGCC 3 cut(s) 267, 378, 411
BspLI GGNNCC 1 cut(s) 361
BspMI ACCTGC 1 cut(s) 84
BspPI GGATC 1 cut(s) 396
BsrFI RCCGGY 2 cut(s) 374, 454
BsrI ACTGG 1 cut(s) 111
BssAI RCCGGY 2 cut(s) 374, 454
BssECI CCNNGG 1 cut(s) 365
BssMI GATC 3 cut(s) 45, 127, 388
BssT1I CCWWGG 1 cut(s) 365
Bst2UI CCWGG 1 cut(s) 40
Bst4CI ACNGT 2 cut(s) 400, 442
Bst6I CTCTTC 2 cut(s) 24, 25
BstBAI YACGTR 1 cut(s) 59
BstC8I GCNNGC 2 cut(s) 148, 456
BstDEI CTNAG 1 cut(s) 198
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 2 cut(s) 164, 251
BstKTI GATC 3 cut(s) 48, 130, 391
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 3 cut(s) 45, 127, 388
BstMWI GCNNNNNNNGC 2 cut(s) 85, 143
BstNI CCWGG 1 cut(s) 40
BstSCI CCNGG 2 cut(s) 38, 486
BstSNI TACGTA 1 cut(s) 59
BstV1I GCAGC 2 cut(s) 258, 481
BstV2I GAAGAC 2 cut(s) 336, 377
BsuRI GGCC 3 cut(s) 267, 378, 411
BtsIMutI CAGTG 1 cut(s) 219
BveI ACCTGC 1 cut(s) 84
Cac8I GCNNGC 2 cut(s) 148, 456
CfoI GCGC 2 cut(s) 164, 251
Cfr10I RCCGGY 2 cut(s) 374, 454
Cfr13I GGNCC 3 cut(s) 265, 400, 409
CpoI CGGWCCG 1 cut(s) 400
CseI GACGC 1 cut(s) 519
Csp6I GTAC 1 cut(s) 258
CspI CGGWCCG 1 cut(s) 400
CviAII CATG 1 cut(s) 184
CviJI RGCY 7 cut(s) 38, 137, 146, 267, 275, 378, 411
CviKI_1 RGCY 7 cut(s) 38, 137, 146, 267, 275, 378, 411
CviQI GTAC 1 cut(s) 258
DdeI CTNAG 1 cut(s) 198
DpnI GATC 3 cut(s) 47, 129, 390
DpnII GATC 3 cut(s) 45, 127, 388
DrdI GACNNNNNNGTC 1 cut(s) 276
DseDI GACNNNNNNGTC 1 cut(s) 276
EaeI YGGCCR 1 cut(s) 376
Eam1104I CTCTTC 2 cut(s) 24, 25
EarI CTCTTC 2 cut(s) 24, 25
EciI GGCGGA 1 cut(s) 409
Ecl136II GAGCTC 1 cut(s) 137
Eco105I TACGTA 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 365
Eco24I GRGCYC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 400
Eco47III AGCGCT 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 137
Eco57I CTGAAG 2 cut(s) 48, 195
EcoICRI GAGCTC 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 365
EcoT38I GRGCYC 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 365
Esp3I CGTCTC 1 cut(s) 284
FaeI CATG 1 cut(s) 187
FaiI YATR 5 cut(s) 10, 56, 68, 185, 227
FalI AAGNNNNNCTT 1 cut(s) 29
FatI CATG 1 cut(s) 183
FbaI TGATCA 1 cut(s) 45
Fnu4HI GCNGC 2 cut(s) 247, 470
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 2 cut(s) 247, 470
FspBI CTAG 1 cut(s) 188
GlaI GCGC 2 cut(s) 163, 250
GluI GCNGC 2 cut(s) 247, 470
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 3 cut(s) 267, 378, 411
HapII CCGG 4 cut(s) 375, 423, 455, 488
HgaI GACGC 1 cut(s) 519
HhaI GCGC 2 cut(s) 164, 251
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 2 cut(s) 162, 249
HinP1I GCGC 2 cut(s) 162, 249
HinfI GANTC 5 cut(s) 217, 238, 383, 449, 496
HpaII CCGG 4 cut(s) 375, 423, 455, 488
HphI GGTGA 1 cut(s) 430
Hpy188I TCNGA 4 cut(s) 28, 388, 404, 509
Hpy188III TCNNGA 1 cut(s) 392
Hpy99I CGWCG 2 cut(s) 352, 358
HpyCH4III ACNGT 2 cut(s) 400, 442
HpyCH4IV ACGT 2 cut(s) 58, 341
HpyCH4V TGCA 1 cut(s) 246
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 143
HpyF3I CTNAG 1 cut(s) 198
HpySE526I ACGT 2 cut(s) 58, 341
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 2 cut(s) 162, 249
KroI GCCGGC 1 cut(s) 454
KroNI GCCGGC 1 cut(s) 456
Ksp22I TGATCA 1 cut(s) 45
Kzo9I GATC 3 cut(s) 45, 127, 388
LmnI GCTCC 1 cut(s) 518
LpnPI CCDG 8 cut(s) 25, 52, 89, 124, 388, 436, 468, 501
Lsp1109I GCAGC 2 cut(s) 258, 481
MaeI CTAG 1 cut(s) 188
MaeII ACGT 2 cut(s) 58, 341
MaeIII GTNAC 3 cut(s) 113, 214, 436
MalI GATC 3 cut(s) 47, 129, 390
MboI GATC 3 cut(s) 45, 127, 388
MboII GAAGA 7 cut(s) 5, 12, 41, 122, 283, 336, 382
MhlI GDGCHC 2 cut(s) 139, 507
MluCI AATT 1 cut(s) 95
MlyI GAGTC 2 cut(s) 211, 443
MnlI CCTC 9 cut(s) 34, 305, 326, 332, 338, 400, 514, 517, 523
MreI CGCCGGCG 1 cut(s) 454
MroNI GCCGGC 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 178
MseI TTAA 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 212
MspI CCGG 4 cut(s) 375, 423, 455, 488
MspR9I CCNGG 2 cut(s) 40, 488
Mva1269I GAATGC 1 cut(s) 164
MvaI CCWGG 1 cut(s) 40
MwoI GCNNNNNNNGC 2 cut(s) 85, 143
NaeI GCCGGC 1 cut(s) 456
NciI CCSGG 1 cut(s) 488
NdeII GATC 3 cut(s) 45, 127, 388
NgoMIV GCCGGC 1 cut(s) 454
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 1 cut(s) 361
NmuCI GTSAC 2 cut(s) 214, 436
OliI CACNNNNGTG 1 cut(s) 212
PctI GAATGC 1 cut(s) 164
PdiI GCCGGC 1 cut(s) 456
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 3 cut(s) 238, 383, 496
PkrI GCNGC 2 cut(s) 248, 471
PleI GAGTC 2 cut(s) 211, 443
PpsI GAGTC 2 cut(s) 211, 443
Ppu21I YACGTR 1 cut(s) 59
Psp124BI GAGCTC 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 38
PspGI CCWGG 1 cut(s) 38
PspN4I GGNNCC 1 cut(s) 361
PspPI GGNCC 3 cut(s) 265, 400, 409
RsaI GTAC 1 cut(s) 259
RsaNI GTAC 1 cut(s) 258
RseI CAYNNNNRTG 1 cut(s) 212
Rsr2I CGGWCCG 1 cut(s) 400
RsrII CGGWCCG 1 cut(s) 400
SacI GAGCTC 1 cut(s) 139
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 2 cut(s) 247, 470
Sau3AI GATC 3 cut(s) 45, 127, 388
Sau96I GGNCC 3 cut(s) 265, 400, 409
SchI GAGTC 2 cut(s) 211, 443
ScrFI CCNGG 2 cut(s) 40, 488
SduI GDGCHC 2 cut(s) 139, 507
SgrAI CRCCGGYG 1 cut(s) 454
SinI GGWCC 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 212
SnaBI TACGTA 1 cut(s) 59
Sse9I AATT 1 cut(s) 95
SsiI CCGC 3 cut(s) 309, 420, 484
SspMI CTAG 1 cut(s) 188
SstI GAGCTC 1 cut(s) 139
StyD4I CCNGG 2 cut(s) 38, 486
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 2 cut(s) 400, 442
TaiI ACGT 2 cut(s) 61, 344
TaqI TCGA 3 cut(s) 133, 391, 447
TasI AATT 1 cut(s) 95
TatI WGTACW 1 cut(s) 257
TfiI GAWTC 3 cut(s) 238, 383, 496
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TscAI CASTG 1 cut(s) 219
TseFI GTSAC 2 cut(s) 214, 436
TseI GCWGC 2 cut(s) 246, 469
Tsp45I GTSAC 2 cut(s) 214, 436
TspDTI ATGAA 1 cut(s) 218
TspRI CASTG 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 400
XapI RAATTY 1 cut(s) 95
XcmI CCANNNNNNNNNTGG 2 cut(s) 118, 523
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.