Rroxscaffold_2G00092890

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016373
Physical Location & Seq
Forward (+)
14346351 .. 14350799
4449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00092890.1

Sequence Viewer

Length: 2256 bp
ATGGGTCAAGAACAGTGTGAGCTCCGCCGCAAGCAATGGTCGCTTTGGTCCGAAATGCGAGCGAGAGAGAGGGGGGTAGGGGCTCCACTAAGAGTCCAAGCTCCCCTAATCCGAGCCTCCCATAGTCTCCATTGCCCCAAACCGTCGCGACCCTCTTCCCCGGCCCACTCCCACCTGCTCGCCCACCGTGTCCCTAACCTCGCCTCCCCTCACACCTTCTCACTCCCCTCCCTCCACCACCAAGGGCTCCCCAAAACCTCTCCTTTTCTCTCCTCCAGAGCTGCGAAACCCTACAAGAAGCAACCCAGCTCCACGCCCTGTCCCTCAAGACCGGAGCTTTCACCCAACCCTTTAATCGCTTCCCGCCTCTTCTCTCTCTACGCCGACCCCAAAATCAACCACCTCACCTACGCCCGCTCCGTCTTCACCCAAATCCAAGCCCCCAACTTGGTCTCCTGGAACGTCCTCATCAAATCCCACGTCACCAACCAACGCTCCCACGACGCCGTCGTTTTGTTCCGCGACTTGGTCCACCACCACGCGCCCGATAACTTCACGCTGCCGTGTGTGATCAAGGGCTGTGCGAGGCTGAATGCGGTGGAGGAAGGGAGGCAGATTCATGGGCTGGGCTTGAAAATTGGGCTGGGCTTGGATAAGTTTGTGCAGAGCAGCTTGGTGAGTATGTACTCCAAATGTGGAGAGATTGAGTTTGCGCGGAAGGTGTTCGATGAAATGCGTGAGAGGGATTTGGTGGCGTGGAATTCTTTGATTGATGGGTACGCTAGATGTGGGAGAGTTGATGTTGCAGTGGAGTTGTTTGATCAGATGAAGGAGAGGGATTCGTTTTCGTGGACGGTTTTGGTTGATGGGTTGTCCAAATGTGGGAATGTAGACAAGGCTAGGGAGGTTTTTGATAGGATGCCGGATAAGAATTTGGTTTCCTGGAATGCCATGATCAATGGGTACATGAAAGCTAGGGACTTTGTGACGGCGAGGGAGTTGTTTAGTCAGATGGTGACTAGAGATGTGATAACATGGAATTCGATGATTGCTGGTTATGAGTTTAATGGGAGGTTTGCGGAAGCGTTGGAGTTGTTTCGAGAAGTTTTGGAAGAAGATGTTAGGCCTACTCCTGCTACATTAGTCAGTGTGCTTTCTGCTCTTTCGGGATTAGCTGCTTTAAGTAAGGGGAGATTGGTTCATTGTGTGATGGTGAAACATGGGTTTGAATTGGATGGTGTGCTTGGTACGTCGTTGATAGAAATGTATTATAAGTGTGGAAGCATTGACAGTGCAATGGCTGTGTTTCAGGGTATACAGAGGAAGAAAGTGGGGCATTGGACGGCTATAATTGTTGGTTTAGGAATGCATGGTATGGCTGACCAGGTTCTTCAGCTGTTTCTGGAAATGCGCAAAAATGGGATGGAGCCTCATGCTATAACTTTTATTGGAATCTTGAATGCATGTAGCCATGCTGGATTGGTCGATCTTGGACGTTACTATTTCAATATGATGATAAAGGATTATGGAATTGAACCCACAATTGAGCACTATGGTTGCTTTGTAGACATTTTATGTCGAGCTGGCTGTTTGGATGAGGCAAAGAATGTCATTGGGAGTATGCCAATGAAACCAAACAAAGTTATTTGGATGAGTTTACTTAGTGGCGCCCGGATCCATGGAAATGTTGAAATTGGTGATTATGCAGCTCAACATTTGATAGAGGTGTCTCCAGATACTATTGGATGCTACGTTGTGCTCTCTAACATGTATGCTGCCGCTGATAAGTGGGAGAAAGTTTCACAGGTAAGAGAAATGATGAAAAAGAGAGGTGTCAAAAAGGAGCCAGGATGCAGTAGCATAGAGCACAGAGGTATGCTTCATGAGTTTATTGTTGGTGACAAATCCCATCCTCAGACAAAAGAGATATATTCCAAGTTGAGTGAGGTGAGAGAGAAACTAAAGTCTGCAGGACATGTTCCTGACACAAGCCAAGTTTTATTGTGTCTTGAGGATGAAAAGGAGAAGGAAGCTGAACTGGAAAACCATAGCGAGAGATTGGCTATTGCATTTGGTCTAATTAATCTGGAACCTAGGAGTCCTATTCGCATCATAAAGAATCTTCGTGTCTGTAATGATTGCCATTCTGTCACTAAACTCCTTTCCAAGATCTATAATCGTGAGATTGTTGTCAGAGATAATAGCCGTTTCCATCACTTCGCCAATGGGTCATGTTCTTGCAAAGATTTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR

Protein Analysis

751

Amino Acids

84.43

Weight (kDa)

8.76

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 148 - 196 3.4e-06 PPR repeat family
PPR_1 PF12854 248 - 277 2.2e-09 PPR repeat
PPR_2 PF13041 249 - 279 1.8e-08 PPR repeat family
PPR PF01535 251 - 279 2.2e-09 PPR repeat
PPR_1 PF12854 279 - 307 7.9e-06 PPR repeat
PPR PF01535 282 - 311 5.6e-07 PPR repeat
PPR_2 PF13041 311 - 342 2.5e-06 PPR repeat family
PPR PF01535 313 - 342 1.1e-06 PPR repeat
PPR_2 PF13041 341 - 388 4.9e-08 PPR repeat family
PPR PF01535 344 - 373 2.3e-06 PPR repeat
PPR_2 PF13041 444 - 490 2e-06 PPR repeat family
E_motif PF20431 560 - 622 1.2e-19 E motif
Eplus_motif PF20430 628 - 651 4.7e-09 E+ motif
DYW_deaminase PF14432 658 - 751 6.1e-38 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016373)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g41070
malus_domestica MD09G1122400.v1.1 MD17G1113300.v1.1
prunus_persica Prupe.3G201100_v2.0.a1
pyrus_communis pycom09g04680
rosa_chinensis RchiOBHm_Chr2g0156631
rosa_laevigata RLG00000020926
rosa_multiflora Rmu_co8328357.1_g000001
rosa_roxburghii Rroxscaffold_2G00092890
rosa_rugosa Rorug02G0454800
rosa_samantha Rh2AG520400 Rh2BG533400 Rh2CG505400 Rh2DG542200
rosa_wichuraiana Rw2G042940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1272
AarI CACCTGC 1 cut(s) 183
Acc16I TGCGCA 1 cut(s) 1412
Acc36I ACCTGC 1 cut(s) 183
AccB1I GGYRCC 1 cut(s) 1667
AccBSI CCGCTC 1 cut(s) 417
AccI GTMKAC 3 cut(s) 891, 1315, 1566
AccII CGCG 4 cut(s) 148, 522, 542, 715
AciI CCGC 9 cut(s) 25, 28, 364, 415, 520, 596, 715, 1079, 1779
AclWI GGATC 2 cut(s) 1669, 1682
AcsI RAATTY 3 cut(s) 760, 931, 1039
AcuI CTGAAG 1 cut(s) 1376
AcyI GRCGYC 2 cut(s) 504, 1668
AfaI GTAC 4 cut(s) 686, 779, 965, 1249
AfiI CCNNNNNNNGG 3 cut(s) 448, 526, 882
AflIII ACRYGT 2 cut(s) 1767, 1975
AgsI TTSAA 6 cut(s) 634, 1229, 1459, 1507, 1535, 1691
AjiI CACGTC 1 cut(s) 481
AjnI CCWGG 4 cut(s) 455, 941, 1383, 1846
Alw21I GWGCWC 4 cut(s) 24, 1551, 1761, 1869
Alw26I GTCTC 3 cut(s) 131, 457, 1734
AlwI GGATC 2 cut(s) 1669, 1682
AoxI GGCC 2 cut(s) 162, 1124
ApeKI GCWGC 6 cut(s) 281, 559, 669, 1175, 1706, 1775
ApoI RAATTY 3 cut(s) 760, 931, 1039
ArsI GACNNNNNNTTYG 2 cut(s) 465, 497
AseI ATTAAT 1 cut(s) 2082
Asp700I GAANNNNTTC 1 cut(s) 722
AspA2I CCTAGG 1 cut(s) 2093
AspLEI GCGC 4 cut(s) 544, 715, 1413, 1670
AspS9I GGNCC 3 cut(s) 48, 163, 529
AsuC2I CCSGG 2 cut(s) 161, 1672
AvaII GGWCC 2 cut(s) 48, 529
AvrII CCTAGG 1 cut(s) 2093
BamHI GGATCC 1 cut(s) 1674
BanI GGYRCC 1 cut(s) 1667
BanII GRGCYC 3 cut(s) 24, 85, 249
BbsI GAAGAC 1 cut(s) 415
Bbv12I GWGCWC 4 cut(s) 24, 1551, 1761, 1869
BbvI GCAGC 6 cut(s) 268, 546, 681, 1162, 1718, 1762
BccI CCATC 8 cut(s) 767, 860, 1006, 1204, 1229, 1417, 1917, 2220
BceAI ACGGC 5 cut(s) 491, 547, 1005, 1359, 2190
BciT130I CCWGG 4 cut(s) 457, 943, 1385, 1848
BclI TGATCA 3 cut(s) 570, 820, 954
BcnI CCSGG 2 cut(s) 161, 1672
BcoDI GTCTC 3 cut(s) 131, 457, 1734
BfaI CTAG 5 cut(s) 783, 900, 975, 1020, 2094
BfmI CTRYAG 1 cut(s) 1968
BfoI RGCGCY 1 cut(s) 1671
BfuAI ACCTGC 1 cut(s) 183
BglII AGATCT 1 cut(s) 2169
BisI GCNGC 8 cut(s) 28, 282, 560, 670, 1176, 1707, 1776, 1779
BlnI CCTAGG 1 cut(s) 2093
BlsI GCNGC 8 cut(s) 29, 283, 561, 671, 1177, 1708, 1777, 1780
Bme1390I CCNGG 6 cut(s) 161, 457, 943, 1385, 1672, 1848
Bme18I GGWCC 2 cut(s) 48, 529
BmgBI CACGTC 1 cut(s) 481
BmgT120I GGNCC 3 cut(s) 48, 163, 529
BmiI GGNNCC 7 cut(s) 84, 248, 1428, 1669, 1676, 1845, 2091
BmrFI CCNGG 6 cut(s) 161, 457, 943, 1385, 1672, 1848
BmsI GCATC 4 cut(s) 909, 1736, 1841, 2118
BpiI GAAGAC 1 cut(s) 415
BpmI CTGGAG 2 cut(s) 259, 1716
BpuEI CTTGAG 2 cut(s) 310, 2030
BpuMI CCSGG 2 cut(s) 161, 1672
BsaHI GRCGYC 2 cut(s) 504, 1668
BsaI GGTCTC 1 cut(s) 457
BsaJI CCNNGG 4 cut(s) 159, 241, 1678, 2093
BsaWI WCCGGW 1 cut(s) 331
BsaXI ACNNNNNCTCC 8 cut(s) 188, 218, 401, 431, 437, 467, 479, 509
Bsc4I CCNNNNNNNGG 3 cut(s) 448, 526, 882
Bse1I ACTGG 1 cut(s) 2043
Bse3DI GCAATG 3 cut(s) 41, 130, 1302
BseBI CCWGG 4 cut(s) 457, 943, 1385, 1848
BseDI CCNNGG 4 cut(s) 159, 241, 1678, 2093
BseGI GGATG 9 cut(s) 924, 1240, 1428, 1600, 1656, 1751, 1856, 1909, 2020
BseLI CCNNNNNNNGG 3 cut(s) 448, 526, 882
BseMI GCAATG 3 cut(s) 41, 130, 1302
BseMII CTCAG 1 cut(s) 1928
BseNI ACTGG 1 cut(s) 2043
BseRI GAGGAG 1 cut(s) 262
BseXI GCAGC 6 cut(s) 268, 546, 681, 1162, 1718, 1762
BseYI CCCAGC 3 cut(s) 305, 625, 643
BsgI GTGCAG 1 cut(s) 683
Bsh1236I CGCG 4 cut(s) 148, 522, 542, 715
BshFI GGCC 2 cut(s) 164, 1126
BshNI GGYRCC 1 cut(s) 1667
BsiHKAI GWGCWC 4 cut(s) 24, 1551, 1761, 1869
BsiSI CCGG 4 cut(s) 161, 332, 923, 1672
BslFI GGGAC 3 cut(s) 176, 306, 992
BslI CCNNNNNNNGG 3 cut(s) 448, 526, 882
BsmAI GTCTC 3 cut(s) 131, 457, 1734
BsmFI GGGAC 3 cut(s) 176, 306, 992
BsmI GAATGC 4 cut(s) 598, 952, 1371, 1465
BsnI GGCC 2 cut(s) 164, 1126
Bso31I GGTCTC 1 cut(s) 457
Bsp1286I GDGCHC 6 cut(s) 24, 85, 249, 1551, 1761, 1869
Bsp143I GATC 6 cut(s) 570, 820, 954, 1486, 1674, 2169
Bsp19I CCATGG 1 cut(s) 1678
Bsp68I TCGCGA 1 cut(s) 148
BspACI CCGC 9 cut(s) 25, 28, 364, 415, 520, 596, 715, 1079, 1779
BspANI GGCC 2 cut(s) 164, 1126
BspCNI CTCAG 1 cut(s) 1927
BspFNI CGCG 4 cut(s) 148, 522, 542, 715
BspHI TCATGA 1 cut(s) 1882
BspLI GGNNCC 7 cut(s) 84, 248, 1428, 1669, 1676, 1845, 2091
BspMAI CTGCAG 1 cut(s) 1972
BspMI ACCTGC 1 cut(s) 183
BspPI GGATC 2 cut(s) 1669, 1682
BspT107I GGYRCC 1 cut(s) 1667
BspTNI GGTCTC 1 cut(s) 457
BsrBI CCGCTC 1 cut(s) 417
BsrDI GCAATG 3 cut(s) 41, 130, 1302
BsrI ACTGG 1 cut(s) 2043
BssECI CCNNGG 4 cut(s) 159, 241, 1678, 2093
BssMI GATC 6 cut(s) 570, 820, 954, 1486, 1674, 2169
BssNAI GTATAC 1 cut(s) 1316
BssNI GRCGYC 2 cut(s) 504, 1668
BssT1I CCWWGG 3 cut(s) 241, 1678, 2093
Bst1107I GTATAC 1 cut(s) 1316
Bst2UI CCWGG 4 cut(s) 457, 943, 1385, 1848
Bst4CI ACNGT 5 cut(s) 15, 144, 188, 856, 1292
Bst6I CTCTTC 2 cut(s) 160, 374
BstACI GRCGYC 2 cut(s) 504, 1668
BstC8I GCNNGC 5 cut(s) 32, 60, 180, 415, 1585
BstDEI CTNAG 3 cut(s) 89, 1661, 1914
BstDSI CCRYGG 1 cut(s) 1678
BstF5I GGATG 9 cut(s) 924, 1240, 1428, 1600, 1656, 1751, 1856, 1909, 2020
BstFNI CGCG 4 cut(s) 148, 522, 542, 715
BstH2I RGCGCY 1 cut(s) 1671
BstHHI GCGC 4 cut(s) 544, 715, 1413, 1670
BstKTI GATC 6 cut(s) 573, 823, 957, 1489, 1677, 2172
BstMAI GTCTC 3 cut(s) 131, 457, 1734
BstMBI GATC 6 cut(s) 570, 820, 954, 1486, 1674, 2169
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstNI CCWGG 4 cut(s) 457, 943, 1385, 1848
BstNSI RCATGY 3 cut(s) 1467, 1771, 1979
BstSCI CCNGG 6 cut(s) 159, 455, 941, 1383, 1670, 1846
BstSFI CTRYAG 1 cut(s) 1968
BstUI CGCG 4 cut(s) 148, 522, 542, 715
BstV1I GCAGC 6 cut(s) 268, 546, 681, 1162, 1718, 1762
BstV2I GAAGAC 1 cut(s) 415
BstX2I RGATCY 2 cut(s) 1674, 2169
BstYI RGATCY 2 cut(s) 1674, 2169
BstZ17I GTATAC 1 cut(s) 1316
BsuRI GGCC 2 cut(s) 164, 1126
BtgI CCRYGG 1 cut(s) 1678
BtrI CACGTC 1 cut(s) 481
BtsCI GGATG 9 cut(s) 924, 1240, 1428, 1600, 1656, 1751, 1856, 1909, 2020
BtsI GCAGTG 1 cut(s) 813
BtsIMutI CAGTG 4 cut(s) 20, 813, 1153, 1297
BtuMI TCGCGA 1 cut(s) 148
BveI ACCTGC 1 cut(s) 183
Cac8I GCNNGC 5 cut(s) 32, 60, 180, 415, 1585
CciI TCATGA 1 cut(s) 1882
CfoI GCGC 4 cut(s) 544, 715, 1413, 1670
Cfr13I GGNCC 3 cut(s) 48, 163, 529
CseI GACGC 1 cut(s) 512
CsiI ACCWGGT 1 cut(s) 1383
Csp6I GTAC 4 cut(s) 685, 778, 964, 1248
CviQI GTAC 4 cut(s) 685, 778, 964, 1248
DdeI CTNAG 3 cut(s) 89, 1661, 1914
DinI GGCGCC 1 cut(s) 1669
DpnI GATC 6 cut(s) 572, 822, 956, 1488, 1676, 2171
DpnII GATC 6 cut(s) 570, 820, 954, 1486, 1674, 2169
Eam1104I CTCTTC 2 cut(s) 160, 374
EarI CTCTTC 2 cut(s) 160, 374
EciI GGCGGA 1 cut(s) 14
Ecl136II GAGCTC 1 cut(s) 22
Eco130I CCWWGG 3 cut(s) 241, 1678, 2093
Eco147I AGGCCT 1 cut(s) 1126
Eco24I GRGCYC 3 cut(s) 24, 85, 249
Eco31I GGTCTC 1 cut(s) 457
Eco47I GGWCC 2 cut(s) 48, 529
Eco53kI GAGCTC 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 1376
EcoICRI GAGCTC 1 cut(s) 22
EcoRI GAATTC 2 cut(s) 760, 1039
EcoRII CCWGG 4 cut(s) 455, 941, 1383, 1846
EcoT14I CCWWGG 3 cut(s) 241, 1678, 2093
EcoT22I ATGCAT 2 cut(s) 1371, 1465
EcoT38I GRGCYC 3 cut(s) 24, 85, 249
EgeI GGCGCC 1 cut(s) 1669
EheI GGCGCC 1 cut(s) 1669
ErhI CCWWGG 3 cut(s) 241, 1678, 2093
FaqI GGGAC 3 cut(s) 176, 306, 992
FauI CCCGC 2 cut(s) 371, 422
FbaI TGATCA 3 cut(s) 570, 820, 954
FblI GTMKAC 3 cut(s) 891, 1315, 1566
Fnu4HI GCNGC 8 cut(s) 28, 282, 560, 670, 1176, 1707, 1776, 1779
FokI GGATG 9 cut(s) 931, 1247, 1435, 1607, 1663, 1758, 1863, 1896, 2027
FriOI GRGCYC 3 cut(s) 24, 85, 249
Fsp4HI GCNGC 8 cut(s) 28, 282, 560, 670, 1176, 1707, 1776, 1779
FspBI CTAG 5 cut(s) 783, 900, 975, 1020, 2094
FspI TGCGCA 1 cut(s) 1412
GlaI GCGC 4 cut(s) 543, 714, 1412, 1669
GluI GCNGC 8 cut(s) 28, 282, 560, 670, 1176, 1707, 1776, 1779
GsaI CCCAGC 3 cut(s) 309, 629, 647
GsuI CTGGAG 2 cut(s) 259, 1716
HaeII RGCGCY 1 cut(s) 1671
HaeIII GGCC 2 cut(s) 164, 1126
HapII CCGG 4 cut(s) 161, 332, 923, 1672
HgaI GACGC 1 cut(s) 512
HhaI GCGC 4 cut(s) 544, 715, 1413, 1670
Hin1I GRCGYC 2 cut(s) 504, 1668
Hin6I GCGC 4 cut(s) 542, 713, 1411, 1668
HinP1I GCGC 4 cut(s) 542, 713, 1411, 1668
HinfI GANTC 6 cut(s) 93, 616, 839, 1452, 2098, 2119
HpaII CCGG 4 cut(s) 161, 332, 923, 1672
Hpy166II GTNNAC 6 cut(s) 532, 852, 892, 1316, 1567, 1658
Hpy188I TCNGA 6 cut(s) 52, 113, 825, 1011, 1917, 2195
Hpy8I GTNNAC 6 cut(s) 532, 852, 892, 1316, 1567, 1658
Hpy99I CGWCG 4 cut(s) 148, 506, 512, 1255
HpyAV CCTTC 5 cut(s) 226, 599, 712, 823, 2020
HpyCH4III ACNGT 5 cut(s) 15, 144, 188, 856, 1292
HpyCH4IV ACGT 5 cut(s) 462, 480, 1250, 1495, 1752
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
HpyF3I CTNAG 3 cut(s) 89, 1661, 1914
HpySE526I ACGT 5 cut(s) 462, 480, 1250, 1495, 1752
Hsp92I GRCGYC 2 cut(s) 504, 1668
HspAI GCGC 4 cut(s) 542, 713, 1411, 1668
KasI GGCGCC 1 cut(s) 1667
Ksp22I TGATCA 3 cut(s) 570, 820, 954
Kzo9I GATC 6 cut(s) 570, 820, 954, 1486, 1674, 2169
Lsp1109I GCAGC 6 cut(s) 268, 546, 681, 1162, 1718, 1762
LweI GCATC 4 cut(s) 909, 1736, 1841, 2118
MabI ACCWGGT 1 cut(s) 1383
MaeI CTAG 5 cut(s) 783, 900, 975, 1020, 2094
MaeII ACGT 5 cut(s) 462, 480, 1250, 1495, 1752
MaeIII GTNAC 6 cut(s) 481, 985, 1015, 1496, 1898, 2149
MalI GATC 6 cut(s) 572, 822, 956, 1488, 1676, 2171
MbiI CCGCTC 1 cut(s) 417
MboI GATC 6 cut(s) 570, 820, 954, 1486, 1674, 2169
MboII GAAGA 8 cut(s) 147, 361, 415, 1124, 1127, 1336, 1382, 2114
MfeI CAATTG 1 cut(s) 1542
MflI RGATCY 2 cut(s) 1674, 2169
MhlI GDGCHC 6 cut(s) 24, 85, 249, 1551, 1761, 1869
Mly113I GGCGCC 1 cut(s) 1668
MlyI GAGTC 2 cut(s) 102, 2107
MmeI TCCRAC 1 cut(s) 1068
Mph1103I ATGCAT 2 cut(s) 1371, 1465
MroXI GAANNNNTTC 1 cut(s) 722
MseI TTAA 4 cut(s) 353, 1065, 1181, 2082
MslI CAYNNNNRTG 1 cut(s) 1683
MspA1I CMGCKG 2 cut(s) 1396, 1781
MspI CCGG 4 cut(s) 161, 332, 923, 1672
MspR9I CCNGG 6 cut(s) 161, 457, 943, 1385, 1672, 1848
MunI CAATTG 1 cut(s) 1542
Mva1269I GAATGC 4 cut(s) 598, 952, 1371, 1465
MvaI CCWGG 4 cut(s) 457, 943, 1385, 1848
MvnI CGCG 4 cut(s) 148, 522, 542, 715
MwoI GCNNNNNNNGC 1 cut(s) 40
NarI GGCGCC 1 cut(s) 1668
NciI CCSGG 2 cut(s) 161, 1672
NcoI CCATGG 1 cut(s) 1678
NdeII GATC 6 cut(s) 570, 820, 954, 1486, 1674, 2169
NlaIV GGNNCC 7 cut(s) 84, 248, 1428, 1669, 1676, 1845, 2091
NmuCI GTSAC 5 cut(s) 481, 985, 1015, 1898, 2149
NruI TCGCGA 1 cut(s) 148
NsbI TGCGCA 1 cut(s) 1412
NsiI ATGCAT 2 cut(s) 1371, 1465
NspI RCATGY 3 cut(s) 1467, 1771, 1979
PagI TCATGA 1 cut(s) 1882
PaqCI CACCTGC 1 cut(s) 183
PceI AGGCCT 1 cut(s) 1126
PciI ACATGT 2 cut(s) 1767, 1975
PcsI WCGNNNNNNNCGW 2 cut(s) 417, 507
PctI GAATGC 4 cut(s) 598, 952, 1371, 1465
PdmI GAANNNNTTC 1 cut(s) 722
PfeI GAWTC 4 cut(s) 616, 839, 1452, 2119
PflFI GACNNNGTC 2 cut(s) 506, 527
PfoI TCCNGGA 2 cut(s) 455, 941
PkrI GCNGC 8 cut(s) 29, 283, 561, 671, 1177, 1708, 1777, 1780
PleI GAGTC 2 cut(s) 101, 2106
PluTI GGCGCC 1 cut(s) 1671
PpsI GAGTC 2 cut(s) 101, 2106
PscI ACATGT 2 cut(s) 1767, 1975
PshBI ATTAAT 1 cut(s) 2082
PsiI TTATAA 1 cut(s) 1272
Psp124BI GAGCTC 1 cut(s) 24
Psp6I CCWGG 4 cut(s) 455, 941, 1383, 1846
PspFI CCCAGC 3 cut(s) 305, 625, 643
PspGI CCWGG 4 cut(s) 455, 941, 1383, 1846
PspN4I GGNNCC 7 cut(s) 84, 248, 1428, 1669, 1676, 1845, 2091
PspPI GGNCC 3 cut(s) 48, 163, 529
PstI CTGCAG 1 cut(s) 1972
PsuI RGATCY 2 cut(s) 1674, 2169
PsyI GACNNNGTC 2 cut(s) 506, 527
PvuII CAGCTG 1 cut(s) 1396
RruI TCGCGA 1 cut(s) 148
RsaI GTAC 4 cut(s) 686, 779, 965, 1249
RsaNI GTAC 4 cut(s) 685, 778, 964, 1248
RseI CAYNNNNRTG 1 cut(s) 1683
SacI GAGCTC 1 cut(s) 24
SaqAI TTAA 4 cut(s) 353, 1065, 1181, 2082
SatI GCNGC 8 cut(s) 28, 282, 560, 670, 1176, 1707, 1776, 1779
Sau3AI GATC 6 cut(s) 570, 820, 954, 1486, 1674, 2169
Sau96I GGNCC 3 cut(s) 48, 163, 529
SchI GAGTC 2 cut(s) 102, 2107
ScrFI CCNGG 6 cut(s) 161, 457, 943, 1385, 1672, 1848
SduI GDGCHC 6 cut(s) 24, 85, 249, 1551, 1761, 1869
SexAI ACCWGGT 1 cut(s) 1383
SfaNI GCATC 4 cut(s) 909, 1736, 1841, 2118
SfcI CTRYAG 1 cut(s) 1968
SfoI GGCGCC 1 cut(s) 1669
SinI GGWCC 2 cut(s) 48, 529
SmiMI CAYNNNNRTG 1 cut(s) 1683
SmlI CTYRAG 2 cut(s) 325, 2009
SmoI CTYRAG 2 cut(s) 325, 2009
SseBI AGGCCT 1 cut(s) 1126
SsiI CCGC 9 cut(s) 25, 28, 364, 415, 520, 596, 715, 1079, 1779
SspDI GGCGCC 1 cut(s) 1667
SspMI CTAG 5 cut(s) 783, 900, 975, 1020, 2094
SstI GAGCTC 1 cut(s) 24
StuI AGGCCT 1 cut(s) 1126
StyD4I CCNGG 6 cut(s) 159, 455, 941, 1383, 1670, 1846
StyI CCWWGG 3 cut(s) 241, 1678, 2093
TaaI ACNGT 5 cut(s) 15, 144, 188, 856, 1292
TaiI ACGT 5 cut(s) 465, 483, 1253, 1498, 1755
TaqI TCGA 5 cut(s) 726, 1043, 1099, 1485, 1579
TatI WGTACW 1 cut(s) 684
TauI GCSGC 2 cut(s) 30, 1781
TfiI GAWTC 4 cut(s) 616, 839, 1452, 2119
Tru1I TTAA 4 cut(s) 353, 1065, 1181, 2082
Tru9I TTAA 4 cut(s) 353, 1065, 1181, 2082
TscAI CASTG 4 cut(s) 20, 813, 1153, 1297
TseFI GTSAC 5 cut(s) 481, 985, 1015, 1898, 2149
TseI GCWGC 6 cut(s) 281, 559, 669, 1175, 1706, 1775
Tsp45I GTSAC 5 cut(s) 481, 985, 1015, 1898, 2149
TspDTI ATGAA 9 cut(s) 608, 744, 842, 983, 1190, 1643, 1835, 1871, 2031
TspGWI ACGGA 1 cut(s) 409
TspRI CASTG 4 cut(s) 20, 813, 1153, 1297
Tth111I GACNNNGTC 2 cut(s) 506, 527
VpaK11BI GGWCC 2 cut(s) 48, 529
VspI ATTAAT 1 cut(s) 2082
XapI RAATTY 3 cut(s) 760, 931, 1039
XceI RCATGY 3 cut(s) 1467, 1771, 1979
XmaJI CCTAGG 1 cut(s) 2093
XmiI GTMKAC 3 cut(s) 891, 1315, 1566
XmnI GAANNNNTTC 1 cut(s) 722
XspI CTAG 5 cut(s) 783, 900, 975, 1020, 2094
Zsp2I ATGCAT 2 cut(s) 1371, 1465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.