Rroxscaffold_2G00121580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016420
Physical Location & Seq
Reverse (-)
54223068 .. 54230143
7076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00121580.1

Sequence Viewer

Length: 684 bp
ATGAAGGTAAACAGAGAAGGAAACAGAAAGAGAAATGTTGTTCAAGGAAGAAGACCCCGCGAAATGCGAATCTCTTCTCGAAAGATCTTGTCAAGCTTAACTCGTCGGCATTTTCTGTTCCTTAACGTTCGAATTATGGAAAAGTGGATCTTCGCTCTCTCCATCCACCGAGCAACAAAAGACAATCTTCCCTTAAACGGACGAAGTTTCGGAAACGCTACTACGACAACTCGGCATGCGGATCTCGAATCTCTAAGAGATGAACTCCACGTGGTGATCTTCAAATTTCGTCGGATCGTGATGGAAGCGCTTATCGAAGCATTCTATCAAGGCCAATCAGAAAACCCACGGTGCAATCTGAGGCGTCCAAATTCTCACCGCTTCATCGTAGCCGTTGAAATTCAATTAAGGGTGGCTATAAGTAGGGCTGGATCAGATCAAATGTCCCAGAATGATCGAGAGGCCTCTGGGCTCTTCGACCCGGCGACTGAACCCGGCCATAACTTCCTCTTCCGGCGAGGATACGGCGGTGCACCGGTCACTTTCGACGGCTCTCGTCGTCGTGGTTCCTCCTGTGGTTACGGATCATCCATGCATGGAGTGAGGAGAGAGAATCGAAGCCCTAAAGCTTCGGGTTTTCCGGCGAAGTTTCTGCAATTTCCGGCGATCTCCGGGGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.82

Weight (kDa)

11.48

Isoelectric Point (pI)

68.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 60
AciI CCGC 4 cut(s) 58, 239, 379, 528
AclI AACGTT 1 cut(s) 126
AclWI GGATC 5 cut(s) 155, 249, 302, 439, 592
AcoI YGGCCR 1 cut(s) 496
AcsI RAATTY 3 cut(s) 284, 370, 399
AcvI CACGTG 1 cut(s) 271
AcyI GRCGYC 1 cut(s) 364
AdeI CACNNNGTG 1 cut(s) 274
AfeI AGCGCT 1 cut(s) 309
AfiI CCNNNNNNNGG 1 cut(s) 197
AgeI ACCGGT 1 cut(s) 535
AgsI TTSAA 4 cut(s) 44, 283, 398, 404
AluBI AGCT 2 cut(s) 96, 629
AluI AGCT 2 cut(s) 96, 629
Alw21I GWGCWC 1 cut(s) 535
Alw44I GTGCAC 1 cut(s) 531
AlwI GGATC 5 cut(s) 155, 249, 302, 439, 592
Aor51HI AGCGCT 1 cut(s) 309
AoxI GGCC 3 cut(s) 331, 462, 496
ApaLI GTGCAC 1 cut(s) 531
ApeKI GCWGC 1 cut(s) 677
ApoI RAATTY 3 cut(s) 284, 370, 399
AsiGI ACCGGT 1 cut(s) 535
Asp700I GAANNNNTTC 1 cut(s) 73
AspLEI GCGC 1 cut(s) 310
AsuC2I CCSGG 3 cut(s) 482, 495, 673
AsuHPI GGTGA 2 cut(s) 286, 368
AsuII TTCGAA 1 cut(s) 130
BaeGI GKGCMC 1 cut(s) 535
BaeI ACNNNNGTAYC 2 cut(s) 514, 547
BanII GRGCYC 1 cut(s) 474
BbrPI CACGTG 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 58
Bbv12I GWGCWC 1 cut(s) 535
BbvI GCAGC 1 cut(s) 664
BccI CCATC 2 cut(s) 170, 295
BceAI ACGGC 3 cut(s) 377, 541, 565
BcgI CGANNNNNNTGC 2 cut(s) 634, 668
BciVI GTATCC 1 cut(s) 515
BcnI CCSGG 3 cut(s) 482, 495, 673
BfoI RGCGCY 1 cut(s) 311
BfuI GTATCC 1 cut(s) 515
BglII AGATCT 1 cut(s) 84
BisI GCNGC 1 cut(s) 678
BlsI GCNGC 1 cut(s) 679
Bme1390I CCNGG 3 cut(s) 482, 495, 673
BmiI GGNNCC 1 cut(s) 568
BmrFI CCNGG 3 cut(s) 482, 495, 673
BpiI GAAGAC 1 cut(s) 58
BplI GAGNNNNNCTC 2 cut(s) 249, 281
Bpu14I TTCGAA 1 cut(s) 130
BpuMI CCSGG 3 cut(s) 482, 495, 673
BsaAI YACGTR 1 cut(s) 271
BsaHI GRCGYC 1 cut(s) 364
BsaJI CCNNGG 2 cut(s) 347, 672
BsaWI WCCGGW 1 cut(s) 535
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse118I RCCGGY 1 cut(s) 535
BseDI CCNNGG 2 cut(s) 347, 672
BseGI GGATG 2 cut(s) 162, 587
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMII CTCAG 1 cut(s) 350
BseRI GAGGAG 1 cut(s) 619
BseSI GKGCMC 1 cut(s) 535
BseXI GCAGC 1 cut(s) 664
Bsh1236I CGCG 1 cut(s) 60
BshFI GGCC 3 cut(s) 333, 464, 498
BshTI ACCGGT 1 cut(s) 535
BsiHKAI GWGCWC 1 cut(s) 535
BsiSI CCGG 7 cut(s) 482, 495, 514, 536, 641, 662, 672
BslFI GGGAC 1 cut(s) 430
BslI CCNNNNNNNGG 1 cut(s) 197
BsmFI GGGAC 1 cut(s) 430
BsmI GAATGC 1 cut(s) 320
BsnI GGCC 3 cut(s) 333, 464, 498
Bsp119I TTCGAA 1 cut(s) 130
Bsp1286I GDGCHC 2 cut(s) 474, 535
BspACI CCGC 4 cut(s) 58, 239, 379, 528
BspANI GGCC 3 cut(s) 333, 464, 498
BspCNI CTCAG 1 cut(s) 351
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 1 cut(s) 568
BspPI GGATC 5 cut(s) 155, 249, 302, 439, 592
BspQI GCTCTTC 1 cut(s) 479
BspT104I TTCGAA 1 cut(s) 130
BsrFI RCCGGY 1 cut(s) 535
BssAI RCCGGY 1 cut(s) 535
BssECI CCNNGG 2 cut(s) 347, 672
BssNI GRCGYC 1 cut(s) 364
Bst4CI ACNGT 1 cut(s) 351
Bst6I CTCTTC 3 cut(s) 79, 479, 515
BstACI GRCGYC 1 cut(s) 364
BstBAI YACGTR 1 cut(s) 271
BstBI TTCGAA 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 2 cut(s) 254, 359
BstDSI CCRYGG 1 cut(s) 347
BstF5I GGATG 2 cut(s) 162, 587
BstFNI CGCG 1 cut(s) 60
BstH2I RGCGCY 1 cut(s) 311
BstHHI GCGC 1 cut(s) 310
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 3 cut(s) 480, 493, 671
BstSLI GKGCMC 1 cut(s) 535
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 664
BstV2I GAAGAC 1 cut(s) 58
BstX2I RGATCY 3 cut(s) 84, 147, 241
BstYI RGATCY 3 cut(s) 84, 147, 241
BsuI GTATCC 1 cut(s) 515
BsuRI GGCC 3 cut(s) 333, 464, 498
BtgI CCRYGG 1 cut(s) 347
BtsCI GGATG 2 cut(s) 162, 587
Cac8I GCNNGC 1 cut(s) 237
CfoI GCGC 1 cut(s) 310
Cfr10I RCCGGY 1 cut(s) 535
CseI GACGC 1 cut(s) 353
CspAI ACCGGT 1 cut(s) 535
CviAII CATG 4 cut(s) 236, 592, 596, 681
DdeI CTNAG 2 cut(s) 254, 359
DraIII CACNNNGTG 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 496
Eam1104I CTCTTC 3 cut(s) 79, 479, 515
EarI CTCTTC 3 cut(s) 79, 479, 515
Eco147I AGGCCT 1 cut(s) 464
Eco24I GRGCYC 1 cut(s) 474
Eco47III AGCGCT 1 cut(s) 309
Eco72I CACGTG 1 cut(s) 271
EcoT22I ATGCAT 1 cut(s) 597
EcoT38I GRGCYC 1 cut(s) 474
FaeI CATG 4 cut(s) 239, 595, 599, 684
FaiI YATR 7 cut(s) 137, 237, 419, 501, 593, 597, 682
FalI AAGNNNNNCTT 4 cut(s) 134, 166, 171, 203
FaqI GGGAC 1 cut(s) 430
FatI CATG 4 cut(s) 235, 591, 595, 680
FauI CCCGC 1 cut(s) 65
Fnu4HI GCNGC 1 cut(s) 678
FokI GGATG 2 cut(s) 149, 574
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 1 cut(s) 678
GlaI GCGC 1 cut(s) 309
GluI GCNGC 1 cut(s) 678
HaeII RGCGCY 1 cut(s) 311
HaeIII GGCC 3 cut(s) 333, 464, 498
HapII CCGG 7 cut(s) 482, 495, 514, 536, 641, 662, 672
HgaI GACGC 1 cut(s) 353
HhaI GCGC 1 cut(s) 310
Hin1I GRCGYC 1 cut(s) 364
Hin1II CATG 4 cut(s) 239, 595, 599, 684
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HindIII AAGCTT 2 cut(s) 94, 627
HinfI GANTC 3 cut(s) 69, 248, 613
HpaII CCGG 7 cut(s) 482, 495, 514, 536, 641, 662, 672
HphI GGTGA 2 cut(s) 286, 368
Hpy166II GTNNAC 2 cut(s) 10, 533
Hpy188I TCNGA 5 cut(s) 212, 294, 340, 360, 436
Hpy188III TCNNGA 4 cut(s) 78, 245, 298, 458
Hpy8I GTNNAC 2 cut(s) 10, 533
Hpy99I CGWCG 5 cut(s) 108, 294, 551, 561, 564
HpyAV CCTTC 1 cut(s) 11
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4IV ACGT 2 cut(s) 126, 270
HpyCH4V TGCA 5 cut(s) 354, 533, 595, 655, 680
HpyF3I CTNAG 2 cut(s) 254, 359
HpySE526I ACGT 2 cut(s) 126, 270
Hsp92I GRCGYC 1 cut(s) 364
Hsp92II CATG 4 cut(s) 239, 595, 599, 684
HspAI GCGC 1 cut(s) 308
LguI GCTCTTC 1 cut(s) 479
Lsp1109I GCAGC 1 cut(s) 664
MaeII ACGT 2 cut(s) 126, 270
MaeIII GTNAC 2 cut(s) 538, 578
MboII GAAGA 8 cut(s) 60, 63, 66, 142, 179, 271, 466, 502
MflI RGATCY 3 cut(s) 84, 147, 241
MhlI GDGCHC 2 cut(s) 474, 535
MluCI AATT 6 cut(s) 132, 284, 370, 399, 404, 656
MmeI TCCRAC 1 cut(s) 272
MnlI CCTC 7 cut(s) 354, 454, 475, 512, 518, 580, 597
Mph1103I ATGCAT 1 cut(s) 597
MroXI GAANNNNTTC 1 cut(s) 73
MseI TTAA 4 cut(s) 98, 123, 194, 407
MspI CCGG 7 cut(s) 482, 495, 514, 536, 641, 662, 672
MspR9I CCNGG 3 cut(s) 482, 495, 673
Mva1269I GAATGC 1 cut(s) 320
MvnI CGCG 1 cut(s) 60
NciI CCSGG 3 cut(s) 482, 495, 673
NlaIII CATG 4 cut(s) 239, 595, 599, 684
NlaIV GGNNCC 1 cut(s) 568
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 538
NsiI ATGCAT 1 cut(s) 597
NspI RCATGY 1 cut(s) 239
NspV TTCGAA 1 cut(s) 130
PaeI GCATGC 1 cut(s) 239
PceI AGGCCT 1 cut(s) 464
PciSI GCTCTTC 1 cut(s) 479
PctI GAATGC 1 cut(s) 320
PdmI GAANNNNTTC 1 cut(s) 73
PfeI GAWTC 3 cut(s) 69, 248, 613
PinAI ACCGGT 1 cut(s) 535
PkrI GCNGC 1 cut(s) 679
PmaCI CACGTG 1 cut(s) 271
PmlI CACGTG 1 cut(s) 271
Ppu21I YACGTR 1 cut(s) 271
Psp1406I AACGTT 1 cut(s) 126
PspCI CACGTG 1 cut(s) 271
PspN4I GGNNCC 1 cut(s) 568
PsuI RGATCY 3 cut(s) 84, 147, 241
SapI GCTCTTC 1 cut(s) 479
SaqAI TTAA 4 cut(s) 98, 123, 194, 407
SatI GCNGC 1 cut(s) 678
ScrFI CCNGG 3 cut(s) 482, 495, 673
SduI GDGCHC 2 cut(s) 474, 535
SetI ASST 5 cut(s) 9, 98, 129, 273, 631
SfuI TTCGAA 1 cut(s) 130
SphI GCATGC 1 cut(s) 239
Sse9I AATT 6 cut(s) 132, 284, 370, 399, 404, 656
SseBI AGGCCT 1 cut(s) 464
SsiI CCGC 4 cut(s) 58, 239, 379, 528
StuI AGGCCT 1 cut(s) 464
StyD4I CCNGG 3 cut(s) 480, 493, 671
TaaI ACNGT 1 cut(s) 351
TaiI ACGT 2 cut(s) 129, 273
TaqI TCGA 8 cut(s) 79, 130, 246, 315, 457, 477, 546, 616
TasI AATT 6 cut(s) 132, 284, 370, 399, 404, 656
TfiI GAWTC 3 cut(s) 69, 248, 613
Tru1I TTAA 4 cut(s) 98, 123, 194, 407
Tru9I TTAA 4 cut(s) 98, 123, 194, 407
TseFI GTSAC 1 cut(s) 538
TseI GCWGC 1 cut(s) 677
Tsp45I GTSAC 1 cut(s) 538
TspDTI ATGAA 3 cut(s) 17, 276, 373
TspGWI ACGGA 2 cut(s) 213, 597
VneI GTGCAC 1 cut(s) 531
XapI RAATTY 3 cut(s) 284, 370, 399
XceI RCATGY 1 cut(s) 239
XmnI GAANNNNTTC 1 cut(s) 73
Zsp2I ATGCAT 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.