Rroxscaffold_2G00154160

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017854
Physical Location & Seq
Reverse (-)
90846285 .. 90848192
1908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154160.1

Sequence Viewer

Length: 240 bp
ATGGTGGTGTTCCATAGCACGTGGCTCGGGACTACATGGGTCGGCGGCATGGCGGCATGCCCGGGACAACGCAGGTTGGCAACATGGCGGCATGGGTCGGAAAAACTCAACCGGCCGGTGACGTATGACGAGGAGTCCGGAAGAGATGGTGGATTAGGCCCGAATCGGGCTGCCGGGCACGGAGTGCTCCCCTTGCCGGTTGTACGTAGTCCACCATCCCAAGCTCTACGGTGGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

79

Amino Acids

8.65

Weight (kDa)

11.16

Isoelectric Point (pI)

72.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 63
AccIII TCCGGA 1 cut(s) 137
AciI CCGC 3 cut(s) 45, 53, 88
AcoI YGGCCR 1 cut(s) 113
AcvI CACGTG 1 cut(s) 21
AdeI CACNNNGTG 1 cut(s) 184
AfaI GTAC 1 cut(s) 204
AfiI CCNNNNNNNGG 2 cut(s) 166, 196
AhdI GACNNNNNGTC 1 cut(s) 133
AluBI AGCT 1 cut(s) 224
AluI AGCT 1 cut(s) 224
Alw21I GWGCWC 1 cut(s) 189
Ama87I CYCGRG 2 cut(s) 26, 61
Aor13HI TCCGGA 1 cut(s) 137
AoxI GGCC 2 cut(s) 113, 157
ApeKI GCWGC 1 cut(s) 170
AspS9I GGNCC 1 cut(s) 158
AsuC2I CCSGG 3 cut(s) 62, 63, 175
AsuHPI GGTGA 1 cut(s) 130
AvaI CYCGRG 2 cut(s) 26, 61
BaeGI GKGCMC 1 cut(s) 180
BbrPI CACGTG 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 1 cut(s) 157
BccI CCATC 2 cut(s) 140, 223
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BcnI CCSGG 3 cut(s) 62, 63, 175
BfuAI ACCTGC 1 cut(s) 63
BisI GCNGC 4 cut(s) 46, 54, 89, 171
BlsI GCNGC 4 cut(s) 47, 55, 90, 172
Bme1390I CCNGG 3 cut(s) 62, 63, 175
BmeRI GACNNNNNGTC 1 cut(s) 133
BmeT110I CYCGRG 2 cut(s) 26, 61
BmgT120I GGNCC 1 cut(s) 158
BmrFI CCNGG 3 cut(s) 62, 63, 175
BpuMI CCSGG 3 cut(s) 62, 63, 175
BsaAI YACGTR 2 cut(s) 21, 206
BsaJI CCNNGG 1 cut(s) 61
BsaWI WCCGGW 1 cut(s) 137
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 196
Bse118I RCCGGY 3 cut(s) 111, 115, 196
BseAI TCCGGA 1 cut(s) 137
BseDI CCNNGG 1 cut(s) 61
BseGI GGATG 1 cut(s) 215
BseLI CCNNNNNNNGG 2 cut(s) 166, 196
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 180
BseX3I CGGCCG 1 cut(s) 113
BseXI GCAGC 1 cut(s) 157
Bsh1285I CGRYCG 1 cut(s) 116
BshFI GGCC 2 cut(s) 115, 159
BsiEI CGRYCG 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 189
BsiHKCI CYCGRG 2 cut(s) 26, 61
BsiSI CCGG 6 cut(s) 62, 112, 116, 138, 174, 197
BslFI GGGAC 2 cut(s) 43, 78
BslI CCNNNNNNNGG 2 cut(s) 166, 196
BsmFI GGGAC 2 cut(s) 43, 78
BsnI GGCC 2 cut(s) 115, 159
BsoBI CYCGRG 2 cut(s) 26, 61
Bsp1286I GDGCHC 2 cut(s) 180, 189
Bsp13I TCCGGA 1 cut(s) 137
BspACI CCGC 3 cut(s) 45, 53, 88
BspANI GGCC 2 cut(s) 115, 159
BspEI TCCGGA 1 cut(s) 137
BspMI ACCTGC 1 cut(s) 63
BsrFI RCCGGY 3 cut(s) 111, 115, 196
BssAI RCCGGY 3 cut(s) 111, 115, 196
BssECI CCNNGG 1 cut(s) 61
Bst4CI ACNGT 1 cut(s) 231
Bst6I CTCTTC 1 cut(s) 136
BstAPI GCANNNNNTGC 1 cut(s) 184
BstBAI YACGTR 2 cut(s) 21, 206
BstC8I GCNNGC 1 cut(s) 58
BstF5I GGATG 1 cut(s) 215
BstMCI CGRYCG 1 cut(s) 116
BstMWI GCNNNNNNNGC 2 cut(s) 184, 193
BstNSI RCATGY 1 cut(s) 60
BstSCI CCNGG 3 cut(s) 60, 61, 173
BstSLI GKGCMC 1 cut(s) 180
BstSNI TACGTA 1 cut(s) 206
BstV1I GCAGC 1 cut(s) 157
BstZI CGGCCG 1 cut(s) 113
BsuRI GGCC 2 cut(s) 115, 159
BtsCI GGATG 1 cut(s) 215
BveI ACCTGC 1 cut(s) 63
Cac8I GCNNGC 1 cut(s) 58
Cfr10I RCCGGY 3 cut(s) 111, 115, 196
Cfr13I GGNCC 1 cut(s) 158
Cfr9I CCCGGG 1 cut(s) 61
Csp6I GTAC 1 cut(s) 203
CviAII CATG 5 cut(s) 36, 49, 57, 84, 92
CviJI RGCY 5 cut(s) 25, 115, 159, 170, 224
CviKI_1 RGCY 5 cut(s) 25, 115, 159, 170, 224
CviQI GTAC 1 cut(s) 203
DraIII CACNNNGTG 1 cut(s) 184
DriI GACNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 1 cut(s) 113
EagI CGGCCG 1 cut(s) 113
Eam1104I CTCTTC 1 cut(s) 136
Eam1105I GACNNNNNGTC 1 cut(s) 133
EarI CTCTTC 1 cut(s) 136
EclXI CGGCCG 1 cut(s) 113
Eco105I TACGTA 1 cut(s) 206
Eco52I CGGCCG 1 cut(s) 113
Eco72I CACGTG 1 cut(s) 21
Eco88I CYCGRG 2 cut(s) 26, 61
FaeI CATG 5 cut(s) 39, 52, 60, 87, 95
FaiI YATR 7 cut(s) 15, 37, 50, 58, 85, 93, 126
FaqI GGGAC 2 cut(s) 43, 78
FatI CATG 5 cut(s) 35, 48, 56, 83, 91
Fnu4HI GCNGC 4 cut(s) 46, 54, 89, 171
FokI GGATG 1 cut(s) 202
Fsp4HI GCNGC 4 cut(s) 46, 54, 89, 171
GluI GCNGC 4 cut(s) 46, 54, 89, 171
HaeIII GGCC 2 cut(s) 115, 159
HapII CCGG 6 cut(s) 62, 112, 116, 138, 174, 197
Hin1II CATG 5 cut(s) 39, 52, 60, 87, 95
HinfI GANTC 2 cut(s) 134, 163
HpaII CCGG 6 cut(s) 62, 112, 116, 138, 174, 197
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 212
Hpy188I TCNGA 1 cut(s) 100
Hpy188III TCNNGA 2 cut(s) 28, 138
Hpy8I GTNNAC 1 cut(s) 212
HpyCH4III ACNGT 1 cut(s) 231
HpyCH4IV ACGT 3 cut(s) 20, 122, 205
HpyF10VI GCNNNNNNNGC 2 cut(s) 184, 193
HpySE526I ACGT 3 cut(s) 20, 122, 205
Hsp92II CATG 5 cut(s) 39, 52, 60, 87, 95
Kpn2I TCCGGA 1 cut(s) 137
LmnI GCTCC 1 cut(s) 192
LpnPI CCDG 7 cut(s) 58, 75, 125, 129, 151, 187, 210
Lsp1109I GCAGC 1 cut(s) 157
MaeII ACGT 3 cut(s) 20, 122, 205
MaeIII GTNAC 1 cut(s) 118
MboII GAAGA 1 cut(s) 153
MhlI GDGCHC 2 cut(s) 180, 189
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 1 cut(s) 124
MroI TCCGGA 1 cut(s) 137
MspI CCGG 6 cut(s) 62, 112, 116, 138, 174, 197
MspR9I CCNGG 3 cut(s) 62, 63, 175
MwoI GCNNNNNNNGC 2 cut(s) 184, 193
NciI CCSGG 3 cut(s) 62, 63, 175
NlaIII CATG 5 cut(s) 39, 52, 60, 87, 95
NmuCI GTSAC 1 cut(s) 118
NspI RCATGY 1 cut(s) 60
PaeI GCATGC 1 cut(s) 60
PfeI GAWTC 1 cut(s) 163
PkrI GCNGC 4 cut(s) 47, 55, 90, 172
PleI GAGTC 1 cut(s) 142
PmaCI CACGTG 1 cut(s) 21
PmlI CACGTG 1 cut(s) 21
PpsI GAGTC 1 cut(s) 142
Ppu21I YACGTR 2 cut(s) 21, 206
PspCI CACGTG 1 cut(s) 21
PspPI GGNCC 1 cut(s) 158
RsaI GTAC 1 cut(s) 204
RsaNI GTAC 1 cut(s) 203
SatI GCNGC 4 cut(s) 46, 54, 89, 171
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 3 cut(s) 62, 63, 175
SduI GDGCHC 2 cut(s) 180, 189
SetI ASST 5 cut(s) 23, 77, 125, 208, 226
SmaI CCCGGG 1 cut(s) 63
SnaBI TACGTA 1 cut(s) 206
SphI GCATGC 1 cut(s) 60
SsiI CCGC 3 cut(s) 45, 53, 88
StyD4I CCNGG 3 cut(s) 60, 61, 173
TaaI ACNGT 1 cut(s) 231
TaiI ACGT 3 cut(s) 23, 125, 208
TauI GCSGC 3 cut(s) 48, 56, 91
TfiI GAWTC 1 cut(s) 163
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 1 cut(s) 170
Tsp45I GTSAC 1 cut(s) 118
TspGWI ACGGA 1 cut(s) 195
TspMI CCCGGG 1 cut(s) 61
XceI RCATGY 1 cut(s) 60
XmaI CCCGGG 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.