Rroxscaffold_2G00155660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008386
Physical Location & Seq
Reverse (-)
92064665 .. 92067524
2860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155660.1

Sequence Viewer

Length: 552 bp
ATGGGCAAGAATCAAGCTTACAAAGCTATGCAGAGAGCCCGGCTGGGATCCAGCTCTGCTGGCCCCGATGAGGTCGAAGACGGCATGGTGGATGGTTCATTTCATTCACCAGAGTGGCATGCTGCTCGTTTGGCTAGCCTTAAAACTTCTCATACAATTACATGGGAGGAGTATAAAAAGAAGCAAAAGGAAGATGAACTGAGAAAAGGGGAAATGGAAAAAGATACCGATAGAATGATGAGAGAATATAGGGCTCAACTGGATGCTGAAAGGGCTCGCAAGCTTGCCAATGGAAGAAACCACTCTAGTAGCAAGTCTGATCGCAAAAAGCATTCAAGAAGGAGATCCTCAGAGTCTAGCTCCTCGACTTCATCGTCAGATTCTTCCAGTAGTGAAGAAGATGACAGAGAATCAAGAAGATCCAAGTCCAGGTCTAAGAGAACAAAAAAGGAAAAGAAGCACAGGTCAAGAACCAAGCACTCTAGCAGTGATGATGACGAGGCTGATGGCCCTGTTCCACTTTCCCGATTCTTTGGCAGTGTCAAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.87

Weight (kDa)

9.84

Isoelectric Point (pI)

62.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 373
AclWI GGATC 4 cut(s) 42, 55, 339, 414
AfiI CCNNNNNNNGG 2 cut(s) 70, 429
AgsI TTSAA 1 cut(s) 336
AjnI CCWGG 1 cut(s) 428
AleI CACNNNNGTG 1 cut(s) 112
AluBI AGCT 6 cut(s) 17, 26, 54, 283, 360, 549
AluI AGCT 6 cut(s) 17, 26, 54, 283, 360, 549
AlwI GGATC 4 cut(s) 42, 55, 339, 414
AoxI GGCC 2 cut(s) 61, 508
ApeKI GCWGC 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 62, 509
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 1 cut(s) 99
AsuNHI GCTAGC 1 cut(s) 134
BamHI GGATCC 1 cut(s) 47
BanII GRGCYC 3 cut(s) 40, 256, 277
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 109
BccI CCATC 2 cut(s) 86, 500
BceAI ACGGC 1 cut(s) 97
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BciT130I CCWGG 1 cut(s) 430
BcnI CCSGG 1 cut(s) 40
BfaI CTAG 5 cut(s) 135, 306, 357, 483, 550
BisI GCNGC 1 cut(s) 123
BlsI GCNGC 1 cut(s) 124
Bme1390I CCNGG 2 cut(s) 40, 430
BmgT120I GGNCC 2 cut(s) 62, 509
BmiI GGNNCC 2 cut(s) 49, 64
BmrFI CCNGG 2 cut(s) 40, 430
BmsI GCATC 1 cut(s) 253
BmtI GCTAGC 1 cut(s) 138
BpiI GAAGAC 1 cut(s) 84
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpuMI CCSGG 1 cut(s) 40
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 429
Bse1I ACTGG 2 cut(s) 264, 387
BseBI CCWGG 1 cut(s) 430
BseGI GGATG 2 cut(s) 97, 268
BseLI CCNNNNNNNGG 2 cut(s) 70, 429
BseMII CTCAG 2 cut(s) 191, 363
BseNI ACTGG 2 cut(s) 264, 387
BseRI GAGGAG 2 cut(s) 182, 352
BseXI GCAGC 1 cut(s) 109
BseYI CCCAGC 1 cut(s) 43
BshFI GGCC 2 cut(s) 63, 510
BsiSI CCGG 1 cut(s) 40
BslI CCNNNNNNNGG 2 cut(s) 70, 429
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 2 cut(s) 63, 510
Bsp1286I GDGCHC 3 cut(s) 40, 256, 277
Bsp143I GATC 4 cut(s) 47, 319, 344, 419
BspANI GGCC 2 cut(s) 63, 510
BspCNI CTCAG 2 cut(s) 192, 362
BspLI GGNNCC 2 cut(s) 49, 64
BspOI GCTAGC 1 cut(s) 138
BspPI GGATC 4 cut(s) 42, 55, 339, 414
BsrI ACTGG 2 cut(s) 264, 387
BssMI GATC 4 cut(s) 47, 319, 344, 419
Bst2UI CCWGG 1 cut(s) 430
BstC8I GCNNGC 6 cut(s) 61, 120, 136, 277, 281, 285
BstDEI CTNAG 3 cut(s) 200, 349, 435
BstF5I GGATG 2 cut(s) 97, 268
BstKTI GATC 4 cut(s) 50, 322, 347, 422
BstMBI GATC 4 cut(s) 47, 319, 344, 419
BstMWI GCNNNNNNNGC 4 cut(s) 23, 60, 131, 272
BstNI CCWGG 1 cut(s) 430
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 2 cut(s) 38, 428
BstV1I GCAGC 1 cut(s) 109
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 3 cut(s) 47, 344, 419
BstYI RGATCY 3 cut(s) 47, 344, 419
BsuRI GGCC 2 cut(s) 63, 510
BtsCI GGATG 2 cut(s) 97, 268
BtsI GCAGTG 2 cut(s) 493, 544
BtsIMutI CAGTG 2 cut(s) 493, 544
Cac8I GCNNGC 6 cut(s) 61, 120, 136, 277, 281, 285
Cfr13I GGNCC 2 cut(s) 62, 509
CviAII CATG 3 cut(s) 85, 119, 162
DdeI CTNAG 3 cut(s) 200, 349, 435
DpnI GATC 4 cut(s) 49, 321, 346, 421
DpnII GATC 4 cut(s) 47, 319, 344, 419
DrdI GACNNNNNNGTC 1 cut(s) 373
DseDI GACNNNNNNGTC 1 cut(s) 373
Eco24I GRGCYC 3 cut(s) 40, 256, 277
EcoRII CCWGG 1 cut(s) 428
EcoT38I GRGCYC 3 cut(s) 40, 256, 277
FaeI CATG 3 cut(s) 88, 122, 165
FaiI YATR 7 cut(s) 29, 86, 120, 153, 163, 174, 249
FatI CATG 3 cut(s) 84, 118, 161
Fnu4HI GCNGC 1 cut(s) 123
FokI GGATG 2 cut(s) 104, 275
FriOI GRGCYC 3 cut(s) 40, 256, 277
Fsp4HI GCNGC 1 cut(s) 123
FspBI CTAG 5 cut(s) 135, 306, 357, 483, 550
GluI GCNGC 1 cut(s) 123
GsaI CCCAGC 1 cut(s) 47
HaeIII GGCC 2 cut(s) 63, 510
HapII CCGG 1 cut(s) 40
Hin1II CATG 3 cut(s) 88, 122, 165
HindIII AAGCTT 2 cut(s) 15, 281
HinfI GANTC 5 cut(s) 10, 353, 380, 410, 528
HpaII CCGG 1 cut(s) 40
HphI GGTGA 1 cut(s) 99
Hpy188I TCNGA 3 cut(s) 319, 352, 379
Hpy188III TCNNGA 5 cut(s) 336, 414, 468, 525, 544
HpyAV CCTTC 1 cut(s) 333
HpyCH4V TGCA 1 cut(s) 31
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 60, 131, 272
HpyF3I CTNAG 3 cut(s) 200, 349, 435
Hsp92II CATG 3 cut(s) 88, 122, 165
Kzo9I GATC 4 cut(s) 47, 319, 344, 419
LmnI GCTCC 1 cut(s) 365
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 1 cut(s) 253
MaeI CTAG 5 cut(s) 135, 306, 357, 483, 550
MalI GATC 4 cut(s) 49, 321, 346, 421
MboI GATC 4 cut(s) 47, 319, 344, 419
MboII GAAGA 7 cut(s) 89, 203, 306, 375, 407, 410, 429
MflI RGATCY 3 cut(s) 47, 344, 419
MhlI GDGCHC 3 cut(s) 40, 256, 277
MluCI AATT 1 cut(s) 156
MlyI GAGTC 1 cut(s) 362
MnlI CCTC 5 cut(s) 64, 160, 358, 373, 493
MseI TTAA 1 cut(s) 141
MslI CAYNNNNRTG 1 cut(s) 112
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 2 cut(s) 40, 430
Mva1269I GAATGC 1 cut(s) 331
MvaI CCWGG 1 cut(s) 430
MwoI GCNNNNNNNGC 4 cut(s) 23, 60, 131, 272
NciI CCSGG 1 cut(s) 40
NdeII GATC 4 cut(s) 47, 319, 344, 419
NheI GCTAGC 1 cut(s) 134
NlaIII CATG 3 cut(s) 88, 122, 165
NlaIV GGNNCC 2 cut(s) 49, 64
NspI RCATGY 1 cut(s) 122
OliI CACNNNNGTG 1 cut(s) 112
PaeI GCATGC 1 cut(s) 122
PcsI WCGNNNNNNNCGW 1 cut(s) 371
PctI GAATGC 1 cut(s) 331
PfeI GAWTC 4 cut(s) 10, 380, 410, 528
PkrI GCNGC 1 cut(s) 124
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
Psp6I CCWGG 1 cut(s) 428
PspFI CCCAGC 1 cut(s) 43
PspGI CCWGG 1 cut(s) 428
PspN4I GGNNCC 2 cut(s) 49, 64
PspPI GGNCC 2 cut(s) 62, 509
PsuI RGATCY 3 cut(s) 47, 344, 419
RseI CAYNNNNRTG 1 cut(s) 112
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 1 cut(s) 123
Sau3AI GATC 4 cut(s) 47, 319, 344, 419
Sau96I GGNCC 2 cut(s) 62, 509
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 2 cut(s) 40, 430
SduI GDGCHC 3 cut(s) 40, 256, 277
SetI ASST 9 cut(s) 19, 28, 56, 75, 285, 362, 434, 467, 551
SfaNI GCATC 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 112
SphI GCATGC 1 cut(s) 122
Sse9I AATT 1 cut(s) 156
SspMI CTAG 5 cut(s) 135, 306, 357, 483, 550
StyD4I CCNGG 2 cut(s) 38, 428
TaqI TCGA 2 cut(s) 75, 365
TasI AATT 1 cut(s) 156
TfiI GAWTC 4 cut(s) 10, 380, 410, 528
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 2 cut(s) 493, 544
TseI GCWGC 1 cut(s) 122
TspDTI ATGAA 4 cut(s) 87, 92, 210, 360
TspRI CASTG 2 cut(s) 493, 544
XceI RCATGY 1 cut(s) 122
XspI CTAG 5 cut(s) 135, 306, 357, 483, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.