Rw2G000510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008386
Physical Location & Seq
Forward (+)
479044 .. 481350
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G000510.1

Sequence Viewer

Length: 609 bp
ATGGGCAAGAATCAAGCTTACAAGGCTATGCAGAGAGCCAGGCTGGGATCCAGCTCCGCTGGCCCCGATGAGGTCGAAGACGGCATGGTGGATGGTTCATTTCATTCACCAGAGTGGCATGCTGCTCGTTTGGCTAGCCTTAAAACTTCTCATACAATTACCTGGGAGGAGTATAAAAAGAAGCAAAAGGAAGATGAACTGAGAAAAGGGGAACTAGAAAAAGATACTGATAGAATGATGAGAGAATACAGGGCTCAACTGGATGCCGAAAGGGCTCGCAAGCTTGCCAATGGAAGAAACCATTCTAGTAGCAAGTCTGATCGCAAAAAAGATAAGAAGGATAAAGATTTAAAGAAACGCAGCAGCAGCAGCAGCAGAAAGAGAAAGCATTCAAGAAGGAGATCCTCAGACTCTAGCTCCTCGACTTCATCTTCAGATTCTTCCAGTAGTGAAGAAGATGACAGAGAATCAAGAAGATCCAAATCCAGGTCTAAGAGAACAAAAAAGGAAAAGAAGCACAGGTCAAGAACCAAGCACTCTAGCAGTGATGATGACGAGGCTGATGGCCCTGTTCCACTTTCCCGATTCTTTGGAAATGTCAAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

23.13

Weight (kDa)

10.07

Isoelectric Point (pI)

64.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0688 PF15772 47 - 200 6.3e-06 UPF0688 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 57
AclWI GGATC 4 cut(s) 42, 55, 396, 471
AcuI CTGAAG 1 cut(s) 417
AfiI CCNNNNNNNGG 2 cut(s) 70, 486
AgsI TTSAA 1 cut(s) 393
AjnI CCWGG 3 cut(s) 38, 161, 485
AleI CACNNNNGTG 1 cut(s) 112
AluBI AGCT 5 cut(s) 17, 54, 283, 417, 606
AluI AGCT 5 cut(s) 17, 54, 283, 417, 606
AlwI GGATC 4 cut(s) 42, 55, 396, 471
AoxI GGCC 2 cut(s) 61, 565
ApeKI GCWGC 6 cut(s) 122, 360, 363, 366, 369, 372
Asp700I GAANNNNTTC 2 cut(s) 301, 388
AspS9I GGNCC 2 cut(s) 62, 566
AsuHPI GGTGA 1 cut(s) 99
AsuNHI GCTAGC 1 cut(s) 134
BamHI GGATCC 1 cut(s) 47
BanII GRGCYC 2 cut(s) 256, 277
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 6 cut(s) 109, 372, 375, 378, 381, 384
BccI CCATC 2 cut(s) 86, 557
BceAI ACGGC 1 cut(s) 97
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BciT130I CCWGG 3 cut(s) 40, 163, 487
BfaI CTAG 6 cut(s) 135, 215, 306, 414, 540, 607
BglI GCCNNNNNGGC 1 cut(s) 272
BisI GCNGC 6 cut(s) 123, 361, 364, 367, 370, 373
BlsI GCNGC 6 cut(s) 124, 362, 365, 368, 371, 374
Bme1390I CCNGG 3 cut(s) 40, 163, 487
BmgT120I GGNCC 2 cut(s) 62, 566
BmiI GGNNCC 2 cut(s) 49, 64
BmrFI CCNGG 3 cut(s) 40, 163, 487
BmsI GCATC 1 cut(s) 253
BmtI GCTAGC 1 cut(s) 138
BpiI GAAGAC 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 481
BsaJI CCNNGG 1 cut(s) 162
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 486
Bse1I ACTGG 2 cut(s) 264, 444
Bse8I GATNNNNATC 1 cut(s) 481
BseBI CCWGG 3 cut(s) 40, 163, 487
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 2 cut(s) 97, 268
BseJI GATNNNNATC 1 cut(s) 481
BseLI CCNNNNNNNGG 2 cut(s) 70, 486
BseMII CTCAG 2 cut(s) 191, 420
BseNI ACTGG 2 cut(s) 264, 444
BseRI GAGGAG 2 cut(s) 182, 409
BseXI GCAGC 6 cut(s) 109, 372, 375, 378, 381, 384
BseYI CCCAGC 1 cut(s) 43
BshFI GGCC 2 cut(s) 63, 567
BslI CCNNNNNNNGG 2 cut(s) 70, 486
BsmI GAATGC 1 cut(s) 388
BsnI GGCC 2 cut(s) 63, 567
Bsp1286I GDGCHC 2 cut(s) 256, 277
Bsp143I GATC 4 cut(s) 47, 319, 401, 476
BspACI CCGC 1 cut(s) 57
BspANI GGCC 2 cut(s) 63, 567
BspCNI CTCAG 2 cut(s) 192, 419
BspLI GGNNCC 2 cut(s) 49, 64
BspOI GCTAGC 1 cut(s) 138
BspPI GGATC 4 cut(s) 42, 55, 396, 471
BsrI ACTGG 2 cut(s) 264, 444
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 4 cut(s) 47, 319, 401, 476
Bst2UI CCWGG 3 cut(s) 40, 163, 487
BstC8I GCNNGC 6 cut(s) 61, 120, 136, 277, 281, 285
BstDEI CTNAG 3 cut(s) 200, 406, 492
BstF5I GGATG 2 cut(s) 97, 268
BstKTI GATC 4 cut(s) 50, 322, 404, 479
BstMBI GATC 4 cut(s) 47, 319, 401, 476
BstMWI GCNNNNNNNGC 7 cut(s) 23, 60, 131, 272, 366, 369, 372
BstNI CCWGG 3 cut(s) 40, 163, 487
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 3 cut(s) 38, 161, 485
BstV1I GCAGC 6 cut(s) 109, 372, 375, 378, 381, 384
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 3 cut(s) 47, 401, 476
BstYI RGATCY 3 cut(s) 47, 401, 476
BsuRI GGCC 2 cut(s) 63, 567
BtsCI GGATG 2 cut(s) 97, 268
BtsI GCAGTG 1 cut(s) 550
BtsIMutI CAGTG 1 cut(s) 550
Cac8I GCNNGC 6 cut(s) 61, 120, 136, 277, 281, 285
Cfr13I GGNCC 2 cut(s) 62, 566
CviAII CATG 2 cut(s) 85, 119
DdeI CTNAG 3 cut(s) 200, 406, 492
DpnI GATC 4 cut(s) 49, 321, 403, 478
DpnII GATC 4 cut(s) 47, 319, 401, 476
DraI TTTAAA 1 cut(s) 351
Eco24I GRGCYC 2 cut(s) 256, 277
Eco57I CTGAAG 1 cut(s) 417
EcoRII CCWGG 3 cut(s) 38, 161, 485
EcoT38I GRGCYC 2 cut(s) 256, 277
FaeI CATG 2 cut(s) 88, 122
FaiI YATR 5 cut(s) 29, 86, 120, 153, 174
FatI CATG 2 cut(s) 84, 118
Fnu4HI GCNGC 6 cut(s) 123, 361, 364, 367, 370, 373
FokI GGATG 2 cut(s) 104, 275
FriOI GRGCYC 2 cut(s) 256, 277
Fsp4HI GCNGC 6 cut(s) 123, 361, 364, 367, 370, 373
FspBI CTAG 6 cut(s) 135, 215, 306, 414, 540, 607
GluI GCNGC 6 cut(s) 123, 361, 364, 367, 370, 373
GsaI CCCAGC 1 cut(s) 47
HaeIII GGCC 2 cut(s) 63, 567
Hin1II CATG 2 cut(s) 88, 122
HindIII AAGCTT 2 cut(s) 15, 281
HinfI GANTC 5 cut(s) 10, 410, 437, 467, 585
HphI GGTGA 1 cut(s) 99
Hpy188I TCNGA 3 cut(s) 319, 409, 436
Hpy188III TCNNGA 5 cut(s) 393, 471, 525, 582, 601
HpyAV CCTTC 2 cut(s) 331, 390
HpyCH4V TGCA 1 cut(s) 31
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 60, 131, 272, 366, 369, 372
HpyF3I CTNAG 3 cut(s) 200, 406, 492
Hsp92II CATG 2 cut(s) 88, 122
Kzo9I GATC 4 cut(s) 47, 319, 401, 476
LmnI GCTCC 2 cut(s) 59, 422
Lsp1109I GCAGC 6 cut(s) 109, 372, 375, 378, 381, 384
LweI GCATC 1 cut(s) 253
MaeI CTAG 6 cut(s) 135, 215, 306, 414, 540, 607
MalI GATC 4 cut(s) 49, 321, 403, 478
MboI GATC 4 cut(s) 47, 319, 401, 476
MboII GAAGA 8 cut(s) 89, 203, 306, 423, 432, 464, 467, 486
MflI RGATCY 3 cut(s) 47, 401, 476
MhlI GDGCHC 2 cut(s) 256, 277
MluCI AATT 1 cut(s) 156
MlyI GAGTC 1 cut(s) 404
MnlI CCTC 5 cut(s) 64, 160, 415, 430, 550
MroXI GAANNNNTTC 2 cut(s) 301, 388
MseI TTAA 2 cut(s) 141, 350
MslI CAYNNNNRTG 1 cut(s) 112
MspA1I CMGCKG 1 cut(s) 59
MspR9I CCNGG 3 cut(s) 40, 163, 487
Mva1269I GAATGC 1 cut(s) 388
MvaI CCWGG 3 cut(s) 40, 163, 487
MwoI GCNNNNNNNGC 7 cut(s) 23, 60, 131, 272, 366, 369, 372
NdeII GATC 4 cut(s) 47, 319, 401, 476
NheI GCTAGC 1 cut(s) 134
NlaIII CATG 2 cut(s) 88, 122
NlaIV GGNNCC 2 cut(s) 49, 64
NspI RCATGY 1 cut(s) 122
OliI CACNNNNGTG 1 cut(s) 112
PaeI GCATGC 1 cut(s) 122
PctI GAATGC 1 cut(s) 388
PdmI GAANNNNTTC 2 cut(s) 301, 388
PfeI GAWTC 4 cut(s) 10, 437, 467, 585
PkrI GCNGC 6 cut(s) 124, 362, 365, 368, 371, 374
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
Psp6I CCWGG 3 cut(s) 38, 161, 485
PspFI CCCAGC 1 cut(s) 43
PspGI CCWGG 3 cut(s) 38, 161, 485
PspN4I GGNNCC 2 cut(s) 49, 64
PspPI GGNCC 2 cut(s) 62, 566
PsuI RGATCY 3 cut(s) 47, 401, 476
RseI CAYNNNNRTG 1 cut(s) 112
SaqAI TTAA 2 cut(s) 141, 350
SatI GCNGC 6 cut(s) 123, 361, 364, 367, 370, 373
Sau3AI GATC 4 cut(s) 47, 319, 401, 476
Sau96I GGNCC 2 cut(s) 62, 566
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 3 cut(s) 40, 163, 487
SduI GDGCHC 2 cut(s) 256, 277
SetI ASST 9 cut(s) 19, 56, 75, 164, 285, 419, 491, 524, 608
SfaNI GCATC 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 112
SphI GCATGC 1 cut(s) 122
Sse9I AATT 1 cut(s) 156
SsiI CCGC 1 cut(s) 57
SspMI CTAG 6 cut(s) 135, 215, 306, 414, 540, 607
StyD4I CCNGG 3 cut(s) 38, 161, 485
TaqI TCGA 2 cut(s) 75, 422
TasI AATT 1 cut(s) 156
TfiI GAWTC 4 cut(s) 10, 437, 467, 585
Tru1I TTAA 2 cut(s) 141, 350
Tru9I TTAA 2 cut(s) 141, 350
TscAI CASTG 1 cut(s) 550
TseI GCWGC 6 cut(s) 122, 360, 363, 366, 369, 372
TspDTI ATGAA 4 cut(s) 87, 92, 210, 417
TspRI CASTG 1 cut(s) 550
XceI RCATGY 1 cut(s) 122
XmnI GAANNNNTTC 2 cut(s) 301, 388
XspI CTAG 6 cut(s) 135, 215, 306, 414, 540, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.