Rroxscaffold_3G00224550

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0017403
Physical Location & Seq
Forward (+)
7747884 .. 7753606
5723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00224550.1

Sequence Viewer

Length: 1314 bp
ATGGATACCTTTATGTCCGAATTCGGGGAGGAGCGGCGGTTGTTAAGGGGTTCGTCGCAATCTCCGGTAATCCAAGTTGATAACACACCGAAGTTTGGGTCACAACCAGATCCTACGATTGCTATGTCTCTTACGGCGTCGGGAAGCCATCATCGTCAACTACCGCTACAGCCGGAGGCCACTTTGTTGAATCGAAACTTGGTTGAATCATTTGCGACAATAGCGGTGTCAATGCTTGATTTCTCTAAGCATGCAAGCAAACACAAGACCATTGATTTTGAGCAGTTCATATTGTATCATTTTGATATCGGTGAATTAATGGCAACTCTTGAGATAGCAGACTTACAAGCTTGTCCACCTAACTGGAAAGTTAGATCCCGAAAACTGTTCTCTCTCGATGAAGATATTGCAAAGACTTTCCGGTGCGTTTGTGAGGTGATCATTGAGGGATCCCATCTCCCATCATCTCATGATTGCAAACACTTCCCTTTTGATGTTTTGCTTCTTCTCATACCGAGGTTGCCTGGTTCTCATATACCAATTTGTTTGTCTTACAAAGTTGTTCGGTGCATTACTGCTGCGATTACCTTTGCTGTGATATTGGTCTATATTGTGTTTCTAACTGCAGCTTATGTCCGCTACAAATTACGTGATTGTTGTTGCATAGAAAAAGACTTAAAGCCTGCAAAGAATGGCCGCAGTGGTTCTCTTTTCCAGAAATATATTGGCAAGATTCATCATGTTGCTTCAATGATGGTGGCAAGAAATGTGGCTTCACGGGAGTTTGACATCACTCATGTTGCACATGTCATCAATTTTGACTTTTCAAAAAGCATAGATGATTATGTTCACGGGATTGGTCAAGCTGGTATGGTAGTGGAGTTTGCTCAACAATCATTGTACTATGGTGGAGTCCGGGCAAAATCTAATTCTCATGTGGTTACTCATTTATGTGCACTAATGCATAATTGTGTCTTCAAAAGTTATTTAAGTCTCTTGAAGCACAGAATGGAGTTCCCTTGCATGCCACATAATGAGATAGCCATCTTTTCTATTTGGATCATTAAGGCATTGCCAGGTTTCAAAGAAGGTGCGATACTACTTTTGCTTGAATTGATTACTATTTGGATAAAATATACAGTTGGTGACAGATTCTTAGGAATAGTGGCAAACATTGGTACGTCATGCTCATGTAATCAAATCTTTGAAGCTGCTCTTAAGCATCTTGTTTTAGCCAACGGGGCTACGGTGGCAAATGGCCGGGAAGTGGAGGTAAACTCGTTGACAAAAAATTGGAGACACATACTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

437

Amino Acids

49.07

Weight (kDa)

8.46

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 34
AciI CCGC 6 cut(s) 34, 37, 164, 224, 637, 697
AclWI GGATC 5 cut(s) 104, 369, 444, 457, 1067
AcoI YGGCCR 2 cut(s) 694, 1258
AcsI RAATTY 1 cut(s) 20
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 2 cut(s) 902, 1180
AfiI CCNNNNNNNGG 3 cut(s) 24, 95, 1267
AflII CTTAAG 1 cut(s) 1217
AflIII ACRYGT 1 cut(s) 805
AgsI TTSAA 9 cut(s) 190, 206, 750, 828, 979, 1000, 1084, 1112, 1208
AjnI CCWGG 2 cut(s) 523, 1075
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
AluBI AGCT 4 cut(s) 350, 629, 866, 1211
AluI AGCT 4 cut(s) 350, 629, 866, 1211
Alw21I GWGCWC 1 cut(s) 958
Alw26I GTCTC 3 cut(s) 132, 998, 1291
Alw44I GTGCAC 1 cut(s) 954
AlwI GGATC 5 cut(s) 104, 369, 444, 457, 1067
AoxI GGCC 3 cut(s) 177, 694, 1258
ApaLI GTGCAC 1 cut(s) 954
ApeKI GCWGC 3 cut(s) 578, 626, 1211
ApoI RAATTY 1 cut(s) 20
ArsI GACNNNNNNTTYG 2 cut(s) 83, 115
AseI ATTAAT 1 cut(s) 317
AsuC2I CCSGG 2 cut(s) 917, 1262
AsuHPI GGTGA 3 cut(s) 323, 448, 1157
BaeGI GKGCMC 1 cut(s) 958
BamHI GGATCC 1 cut(s) 449
BarI GAAGNNNNNNTAC 2 cut(s) 1080, 1112
BbsI GAAGAC 1 cut(s) 967
Bbv12I GWGCWC 1 cut(s) 958
BbvI GCAGC 3 cut(s) 565, 638, 1198
BccI CCATC 5 cut(s) 156, 462, 469, 748, 1052
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 2 cut(s) 525, 1077
BclI TGATCA 1 cut(s) 438
BcnI CCSGG 2 cut(s) 917, 1262
BcoDI GTCTC 3 cut(s) 132, 998, 1291
BfmI CTRYAG 2 cut(s) 167, 624
BfrI CTTAAG 1 cut(s) 1217
BglI GCCNNNNNGGC 1 cut(s) 1241
BisI GCNGC 5 cut(s) 35, 579, 627, 697, 1212
BlsI GCNGC 5 cut(s) 36, 580, 628, 698, 1213
Bme1390I CCNGG 4 cut(s) 525, 917, 1077, 1262
BmiI GGNNCC 1 cut(s) 451
BmrFI CCNGG 4 cut(s) 525, 917, 1077, 1262
BmsI GCATC 1 cut(s) 1231
BpiI GAAGAC 1 cut(s) 967
BplI GAGNNNNNCTC 2 cut(s) 1262, 1294
BpuEI CTTGAG 1 cut(s) 350
BpuMI CCSGG 2 cut(s) 917, 1262
BsaAI YACGTR 1 cut(s) 650
BsaBI GATNNNNATC 1 cut(s) 1043
BsaHI GRCGYC 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 515
BsaWI WCCGGW 2 cut(s) 64, 420
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 95, 1267
Bse1I ACTGG 1 cut(s) 368
Bse3DI GCAATG 1 cut(s) 1070
Bse8I GATNNNNATC 1 cut(s) 1043
BseBI CCWGG 2 cut(s) 525, 1077
BseDI CCNNGG 1 cut(s) 515
BseJI GATNNNNATC 1 cut(s) 1043
BseLI CCNNNNNNNGG 3 cut(s) 24, 95, 1267
BseMI GCAATG 1 cut(s) 1070
BseNI ACTGG 1 cut(s) 368
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 1 cut(s) 958
BseXI GCAGC 3 cut(s) 565, 638, 1198
BshFI GGCC 3 cut(s) 179, 696, 1260
BsiHKAI GWGCWC 1 cut(s) 958
BsiSI CCGG 5 cut(s) 65, 173, 421, 916, 1261
BslI CCNNNNNNNGG 3 cut(s) 24, 95, 1267
BsmAI GTCTC 3 cut(s) 132, 998, 1291
BsnI GGCC 3 cut(s) 179, 696, 1260
Bsp1286I GDGCHC 1 cut(s) 958
Bsp143I GATC 5 cut(s) 109, 374, 438, 449, 1059
BspACI CCGC 6 cut(s) 34, 37, 164, 224, 637, 697
BspANI GGCC 3 cut(s) 179, 696, 1260
BspHI TCATGA 1 cut(s) 469
BspLI GGNNCC 1 cut(s) 451
BspMAI CTGCAG 1 cut(s) 628
BspPI GGATC 5 cut(s) 104, 369, 444, 457, 1067
BspTI CTTAAG 1 cut(s) 1217
BsrBI CCGCTC 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 1070
BsrI ACTGG 1 cut(s) 368
BssECI CCNNGG 1 cut(s) 515
BssMI GATC 5 cut(s) 109, 374, 438, 449, 1059
BssNI GRCGYC 1 cut(s) 137
Bst2UI CCWGG 2 cut(s) 525, 1077
Bst4CI ACNGT 3 cut(s) 387, 1141, 1249
BstACI GRCGYC 1 cut(s) 137
BstAFI CTTAAG 1 cut(s) 1217
BstBAI YACGTR 1 cut(s) 650
BstC8I GCNNGC 4 cut(s) 252, 256, 684, 1025
BstDEI CTNAG 2 cut(s) 246, 1156
BstKTI GATC 5 cut(s) 112, 377, 441, 452, 1062
BstMAI GTCTC 3 cut(s) 132, 998, 1291
BstMBI GATC 5 cut(s) 109, 374, 438, 449, 1059
BstMWI GCNNNNNNNGC 3 cut(s) 221, 1241, 1250
BstNI CCWGG 2 cut(s) 525, 1077
BstNSI RCATGY 3 cut(s) 254, 809, 1027
BstSCI CCNGG 4 cut(s) 523, 915, 1075, 1260
BstSFI CTRYAG 2 cut(s) 167, 624
BstSLI GKGCMC 1 cut(s) 958
BstV1I GCAGC 3 cut(s) 565, 638, 1198
BstV2I GAAGAC 1 cut(s) 967
BstX2I RGATCY 3 cut(s) 109, 374, 449
BstXI CCANNNNNNTGG 1 cut(s) 363
BstYI RGATCY 3 cut(s) 109, 374, 449
BsuRI GGCC 3 cut(s) 179, 696, 1260
BtsI GCAGTG 1 cut(s) 706
BtsIMutI CAGTG 1 cut(s) 706
Cac8I GCNNGC 4 cut(s) 252, 256, 684, 1025
CciI TCATGA 1 cut(s) 469
CseI GACGC 1 cut(s) 126
Csp6I GTAC 2 cut(s) 901, 1179
CspCI CAANNNNNGTGG 4 cut(s) 738, 750, 773, 785
CviAII CATG 9 cut(s) 251, 470, 740, 797, 806, 935, 1024, 1185, 1191
CviQI GTAC 2 cut(s) 901, 1179
DdeI CTNAG 2 cut(s) 246, 1156
DpnI GATC 5 cut(s) 111, 376, 440, 451, 1061
DpnII GATC 5 cut(s) 109, 374, 438, 449, 1059
EaeI YGGCCR 2 cut(s) 694, 1258
Eco32I GATATC 1 cut(s) 307
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 2 cut(s) 523, 1075
EcoRV GATATC 1 cut(s) 307
EcoT22I ATGCAT 1 cut(s) 966
FaeI CATG 9 cut(s) 254, 473, 743, 800, 809, 938, 1027, 1188, 1194
FalI AAGNNNNNCTT 2 cut(s) 1200, 1232
FatI CATG 9 cut(s) 250, 469, 739, 796, 805, 934, 1023, 1184, 1190
FbaI TGATCA 1 cut(s) 438
Fnu4HI GCNGC 5 cut(s) 35, 579, 627, 697, 1212
Fsp4HI GCNGC 5 cut(s) 35, 579, 627, 697, 1212
GluI GCNGC 5 cut(s) 35, 579, 627, 697, 1212
HaeIII GGCC 3 cut(s) 179, 696, 1260
HapII CCGG 5 cut(s) 65, 173, 421, 916, 1261
HgaI GACGC 1 cut(s) 126
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 9 cut(s) 254, 473, 743, 800, 809, 938, 1027, 1188, 1194
HincII GTYRAC 2 cut(s) 158, 1284
HindII GTYRAC 2 cut(s) 158, 1284
HindIII AAGCTT 1 cut(s) 348
HinfI GANTC 5 cut(s) 190, 206, 733, 912, 1152
HpaII CCGG 5 cut(s) 65, 173, 421, 916, 1261
HphI GGTGA 3 cut(s) 323, 448, 1157
Hpy166II GTNNAC 6 cut(s) 158, 356, 850, 956, 1276, 1284
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 7 cut(s) 141, 329, 378, 395, 470, 715, 997
Hpy8I GTNNAC 6 cut(s) 158, 356, 850, 956, 1276, 1284
Hpy99I CGWCG 2 cut(s) 58, 142
HpyAV CCTTC 1 cut(s) 1082
HpyCH4III ACNGT 3 cut(s) 387, 1141, 1249
HpyCH4IV ACGT 2 cut(s) 649, 1181
HpyF10VI GCNNNNNNNGC 3 cut(s) 221, 1241, 1250
HpyF3I CTNAG 2 cut(s) 246, 1156
HpySE526I ACGT 2 cut(s) 649, 1181
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 9 cut(s) 254, 473, 743, 800, 809, 938, 1027, 1188, 1194
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 5 cut(s) 109, 374, 438, 449, 1059
LmnI GCTCC 1 cut(s) 31
Lsp1109I GCAGC 3 cut(s) 565, 638, 1198
LweI GCATC 1 cut(s) 1231
MaeII ACGT 2 cut(s) 649, 1181
MaeIII GTNAC 3 cut(s) 99, 940, 1145
MalI GATC 5 cut(s) 111, 376, 440, 451, 1061
MbiI CCGCTC 1 cut(s) 34
MboI GATC 5 cut(s) 109, 374, 438, 449, 1059
MboII GAAGA 3 cut(s) 413, 497, 967
MflI RGATCY 3 cut(s) 109, 374, 449
MhlI GDGCHC 1 cut(s) 958
MluCI AATT 9 cut(s) 20, 314, 540, 644, 814, 928, 967, 1112, 1291
MlyI GAGTC 1 cut(s) 921
MnlI CCTC 6 cut(s) 22, 169, 427, 439, 510, 1264
Mph1103I ATGCAT 1 cut(s) 966
MseI TTAA 6 cut(s) 44, 317, 677, 989, 1065, 1218
MslI CAYNNNNRTG 4 cut(s) 951, 969, 1189, 1307
MspCI CTTAAG 1 cut(s) 1217
MspI CCGG 5 cut(s) 65, 173, 421, 916, 1261
MspR9I CCNGG 4 cut(s) 525, 917, 1077, 1262
MvaI CCWGG 2 cut(s) 525, 1077
MwoI GCNNNNNNNGC 3 cut(s) 221, 1241, 1250
NciI CCSGG 2 cut(s) 917, 1262
NdeII GATC 5 cut(s) 109, 374, 438, 449, 1059
NlaIII CATG 9 cut(s) 254, 473, 743, 800, 809, 938, 1027, 1188, 1194
NlaIV GGNNCC 1 cut(s) 451
NmuCI GTSAC 2 cut(s) 99, 1145
NsiI ATGCAT 1 cut(s) 966
NspI RCATGY 3 cut(s) 254, 809, 1027
PaeI GCATGC 2 cut(s) 254, 1027
PagI TCATGA 1 cut(s) 469
PciI ACATGT 1 cut(s) 805
PfeI GAWTC 4 cut(s) 190, 206, 733, 1152
PkrI GCNGC 5 cut(s) 36, 580, 628, 698, 1213
PleI GAGTC 1 cut(s) 920
PpsI GAGTC 1 cut(s) 920
Ppu21I YACGTR 1 cut(s) 650
PscI ACATGT 1 cut(s) 805
PshBI ATTAAT 1 cut(s) 317
Psp6I CCWGG 2 cut(s) 523, 1075
PspGI CCWGG 2 cut(s) 523, 1075
PspN4I GGNNCC 1 cut(s) 451
PstI CTGCAG 1 cut(s) 628
PsuI RGATCY 3 cut(s) 109, 374, 449
RsaI GTAC 2 cut(s) 902, 1180
RsaNI GTAC 2 cut(s) 901, 1179
RseI CAYNNNNRTG 4 cut(s) 951, 969, 1189, 1307
SaqAI TTAA 6 cut(s) 44, 317, 677, 989, 1065, 1218
SatI GCNGC 5 cut(s) 35, 579, 627, 697, 1212
Sau3AI GATC 5 cut(s) 109, 374, 438, 449, 1059
SchI GAGTC 1 cut(s) 921
ScrFI CCNGG 4 cut(s) 525, 917, 1077, 1262
SduI GDGCHC 1 cut(s) 958
SfaNI GCATC 1 cut(s) 1231
SfcI CTRYAG 2 cut(s) 167, 624
SmiMI CAYNNNNRTG 4 cut(s) 951, 969, 1189, 1307
SmlI CTYRAG 2 cut(s) 329, 1217
SmoI CTYRAG 2 cut(s) 329, 1217
SphI GCATGC 2 cut(s) 254, 1027
Sse9I AATT 9 cut(s) 20, 314, 540, 644, 814, 928, 967, 1112, 1291
SsiI CCGC 6 cut(s) 34, 37, 164, 224, 637, 697
StyD4I CCNGG 4 cut(s) 523, 915, 1075, 1260
TaaI ACNGT 3 cut(s) 387, 1141, 1249
TaiI ACGT 2 cut(s) 652, 1184
TaqI TCGA 2 cut(s) 193, 396
TasI AATT 9 cut(s) 20, 314, 540, 644, 814, 928, 967, 1112, 1291
TatI WGTACW 1 cut(s) 900
TauI GCSGC 2 cut(s) 37, 699
TfiI GAWTC 4 cut(s) 190, 206, 733, 1152
Tru1I TTAA 6 cut(s) 44, 317, 677, 989, 1065, 1218
Tru9I TTAA 6 cut(s) 44, 317, 677, 989, 1065, 1218
TscAI CASTG 1 cut(s) 706
TseFI GTSAC 2 cut(s) 99, 1145
TseI GCWGC 3 cut(s) 578, 626, 1211
Tsp45I GTSAC 2 cut(s) 99, 1145
TspDTI ATGAA 3 cut(s) 277, 414, 725
TspRI CASTG 1 cut(s) 706
Vha464I CTTAAG 1 cut(s) 1217
VneI GTGCAC 1 cut(s) 954
VspI ATTAAT 1 cut(s) 317
XapI RAATTY 1 cut(s) 20
XceI RCATGY 3 cut(s) 254, 809, 1027
XcmI CCANNNNNNNNNTGG 1 cut(s) 722
Zsp2I ATGCAT 1 cut(s) 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.