Rroxscaffold_3G00239580

Galactinol--sucrose galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0017862
Physical Location & Seq
Reverse (-)
29961111 .. 29961859
749 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00239580.1

Sequence Viewer

Length: 663 bp
ATGATTGCGAGCCAGCAATGGAGACAAAATGTCGCGCCACCTCCTTCCGGTTTGTCAAACATACAGAACCAACTCACCACCATCGACCAAAACTCGGACTCGTTTGAGGTGGGTCGCATCGATAGCAGCTATAGCTTCATGAACATCACAGCCACTCCATCACCAAACGAAACCACCGTGGGCTGCTTCATTGGCTTTGACGCCGACGAGCCCAAAAGCAGCCATGTGGTGCCGCTCGGCAAGCTCGGCGGCATCAAGTTCATGAGCATTTTCCGGTTCAAAGTTTGGTGGACCACCCGGTGGGTCGGAACAACCGGAAACGACGTGCGGCACGAGACTCGGTCCCTTCCGAGCCTCCCTCCAACCCGCACCGACCGCCACATGGTCATGACCGTCGAAAGTGGAGGTGGTGAGAGTCCATTTGAGAAGCTTCAAGCTTCGGAGAAGAAGACACCTCCGCCTATAGTGGACAAGTTTAGTTGGTACACGTGGGATGCGTTTTATCTTAGGGTACACCCCGATAAAGTTTGGGAGGGGGTTAAAGGCCTAGTGGAGGGTGGGTGTCCTCCGGAAATGGTACTCATCGACTACGGTGGCAGTCCATTTGTCACGATGACGACCCCTAACCGACGAAGAAGCCATGAAAAAGACATCTGCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

220

Amino Acids

24.71

Weight (kDa)

6.96

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 45 - 114 5e-21 Raffinose synthase or seed imbibition protein Sip1
Raffinose_syn PF05691 143 - 198 6.1e-19 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 229
AccB7I CCANNNNNTGG 1 cut(s) 300
AccBSI CCGCTC 1 cut(s) 235
AccII CGCG 1 cut(s) 35
AccIII TCCGGA 1 cut(s) 568
AciI CCGC 6 cut(s) 233, 249, 328, 367, 376, 458
AcvI CACGTG 1 cut(s) 489
AcyI GRCGYC 1 cut(s) 201
AdeI CACNNNGTG 1 cut(s) 300
AfaI GTAC 3 cut(s) 485, 513, 579
AfiI CCNNNNNNNGG 5 cut(s) 47, 94, 300, 382, 553
AflIII ACRYGT 1 cut(s) 486
AgsI TTSAA 2 cut(s) 280, 434
AhdI GACNNNNNGTC 1 cut(s) 29
AjiI CACGTC 1 cut(s) 325
AluBI AGCT 5 cut(s) 129, 135, 244, 430, 437
AluI AGCT 5 cut(s) 129, 135, 244, 430, 437
Alw26I GTCTC 2 cut(s) 16, 329
Aor13HI TCCGGA 1 cut(s) 568
AoxI GGCC 1 cut(s) 544
ApeKI GCWGC 3 cut(s) 126, 183, 219
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 2 cut(s) 291, 342
AsuC2I CCSGG 1 cut(s) 298
AsuHPI GGTGA 3 cut(s) 67, 153, 422
AvaII GGWCC 2 cut(s) 291, 342
BanI GGYRCC 1 cut(s) 229
BanII GRGCYC 1 cut(s) 213
BauI CACGAG 1 cut(s) 332
BbrPI CACGTG 1 cut(s) 489
BbsI GAAGAC 1 cut(s) 455
BbvI GCAGC 3 cut(s) 138, 170, 231
BccI CCATC 2 cut(s) 89, 166
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BcnI CCSGG 1 cut(s) 298
BcoDI GTCTC 2 cut(s) 16, 329
BfaI CTAG 1 cut(s) 548
BfmI CTRYAG 2 cut(s) 130, 462
BisI GCNGC 6 cut(s) 127, 184, 220, 233, 250, 329
BlsI GCNGC 6 cut(s) 128, 185, 221, 234, 251, 330
Bme1390I CCNGG 1 cut(s) 298
Bme18I GGWCC 2 cut(s) 291, 342
BmeRI GACNNNNNGTC 1 cut(s) 29
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 2 cut(s) 291, 342
BmiI GGNNCC 2 cut(s) 231, 344
BmrFI CCNGG 1 cut(s) 298
BmsI GCATC 3 cut(s) 126, 261, 484
BpiI GAAGAC 1 cut(s) 455
BplI GAGNNNNNCTC 2 cut(s) 343, 375
BpuMI CCSGG 1 cut(s) 298
Bsa29I ATCGAT 1 cut(s) 120
BsaAI YACGTR 1 cut(s) 489
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 177
BsaWI WCCGGW 4 cut(s) 47, 273, 314, 568
BsaXI ACNNNNNCTCC 4 cut(s) 139, 169, 545, 575
Bsc4I CCNNNNNNNGG 5 cut(s) 47, 94, 300, 382, 553
Bse118I RCCGGY 1 cut(s) 657
Bse3DI GCAATG 1 cut(s) 23
BseAI TCCGGA 1 cut(s) 568
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 1 cut(s) 499
BseLI CCNNNNNNNGG 5 cut(s) 47, 94, 300, 382, 553
BseMI GCAATG 1 cut(s) 23
BseXI GCAGC 3 cut(s) 138, 170, 231
Bsh1236I CGCG 1 cut(s) 35
Bsh1285I CGRYCG 1 cut(s) 376
BshFI GGCC 1 cut(s) 546
BshNI GGYRCC 1 cut(s) 229
BshVI ATCGAT 1 cut(s) 120
BsiEI CGRYCG 1 cut(s) 376
BsiSI CCGG 6 cut(s) 48, 274, 298, 315, 569, 658
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 5 cut(s) 47, 94, 300, 382, 553
BsmAI GTCTC 2 cut(s) 16, 329
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 1 cut(s) 546
Bsp1286I GDGCHC 1 cut(s) 213
Bsp13I TCCGGA 1 cut(s) 568
BspACI CCGC 6 cut(s) 233, 249, 328, 367, 376, 458
BspANI GGCC 1 cut(s) 546
BspDI ATCGAT 1 cut(s) 120
BspEI TCCGGA 1 cut(s) 568
BspFNI CGCG 1 cut(s) 35
BspHI TCATGA 3 cut(s) 138, 261, 387
BspLI GGNNCC 2 cut(s) 231, 344
BspT107I GGYRCC 1 cut(s) 229
BsrBI CCGCTC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 23
BsrFI RCCGGY 1 cut(s) 657
BssAI RCCGGY 1 cut(s) 657
BssECI CCNNGG 1 cut(s) 177
BssNI GRCGYC 1 cut(s) 201
BssSI CACGAG 1 cut(s) 332
Bst2BI CACGAG 1 cut(s) 332
Bst4CI ACNGT 3 cut(s) 178, 394, 593
BstACI GRCGYC 1 cut(s) 201
BstBAI YACGTR 1 cut(s) 489
BstC8I GCNNGC 3 cut(s) 10, 14, 242
BstDEI CTNAG 1 cut(s) 506
BstDSI CCRYGG 1 cut(s) 177
BstENI CCTNNNNNAGG 1 cut(s) 551
BstF5I GGATG 1 cut(s) 499
BstFNI CGCG 1 cut(s) 35
BstHHI GCGC 1 cut(s) 37
BstMAI GTCTC 2 cut(s) 16, 329
BstMCI CGRYCG 1 cut(s) 376
BstMWI GCNNNNNNNGC 6 cut(s) 123, 132, 192, 241, 246, 375
BstSCI CCNGG 1 cut(s) 296
BstSFI CTRYAG 2 cut(s) 130, 462
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 138, 170, 231
BstV2I GAAGAC 1 cut(s) 455
Bsu15I ATCGAT 1 cut(s) 120
BsuRI GGCC 1 cut(s) 546
BsuTUI ATCGAT 1 cut(s) 120
BtgI CCRYGG 1 cut(s) 177
BtrI CACGTC 1 cut(s) 325
BtsCI GGATG 1 cut(s) 499
Cac8I GCNNGC 3 cut(s) 10, 14, 242
CciI TCATGA 3 cut(s) 138, 261, 387
CfoI GCGC 1 cut(s) 37
Cfr10I RCCGGY 1 cut(s) 657
Cfr13I GGNCC 2 cut(s) 291, 342
ClaI ATCGAT 1 cut(s) 120
CseI GACGC 1 cut(s) 209
Csp6I GTAC 3 cut(s) 484, 512, 578
CviAII CATG 6 cut(s) 139, 224, 262, 382, 388, 641
CviQI GTAC 3 cut(s) 484, 512, 578
DdeI CTNAG 1 cut(s) 506
DraIII CACNNNGTG 1 cut(s) 300
DriI GACNNNNNGTC 1 cut(s) 29
Eam1105I GACNNNNNGTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 447
Eco147I AGGCCT 1 cut(s) 546
Eco24I GRGCYC 1 cut(s) 213
Eco47I GGWCC 2 cut(s) 291, 342
Eco72I CACGTG 1 cut(s) 489
EcoNI CCTNNNNNAGG 1 cut(s) 551
EcoT38I GRGCYC 1 cut(s) 213
FaeI CATG 6 cut(s) 142, 227, 265, 385, 391, 644
FaiI YATR 9 cut(s) 62, 132, 140, 225, 263, 383, 389, 464, 642
FaqI GGGAC 1 cut(s) 328
FatI CATG 6 cut(s) 138, 223, 261, 381, 387, 640
FauI CCCGC 1 cut(s) 374
Fnu4HI GCNGC 6 cut(s) 127, 184, 220, 233, 250, 329
FokI GGATG 1 cut(s) 506
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 6 cut(s) 127, 184, 220, 233, 250, 329
FspBI CTAG 1 cut(s) 548
GlaI GCGC 1 cut(s) 36
GluI GCNGC 6 cut(s) 127, 184, 220, 233, 250, 329
HaeIII GGCC 1 cut(s) 546
HapII CCGG 6 cut(s) 48, 274, 298, 315, 569, 658
HgaI GACGC 1 cut(s) 209
HhaI GCGC 1 cut(s) 37
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 6 cut(s) 142, 227, 265, 385, 391, 644
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HindIII AAGCTT 2 cut(s) 428, 435
HinfI GANTC 3 cut(s) 98, 337, 415
HpaII CCGG 6 cut(s) 48, 274, 298, 315, 569, 658
HphI GGTGA 3 cut(s) 67, 153, 422
Hpy166II GTNNAC 4 cut(s) 291, 469, 486, 514
Hpy188I TCNGA 4 cut(s) 97, 308, 351, 442
Hpy188III TCNNGA 5 cut(s) 139, 262, 388, 569, 610
Hpy8I GTNNAC 4 cut(s) 291, 469, 486, 514
Hpy99I CGWCG 4 cut(s) 209, 326, 398, 633
HpyAV CCTTC 2 cut(s) 54, 356
HpyCH4III ACNGT 3 cut(s) 178, 394, 593
HpyCH4IV ACGT 2 cut(s) 324, 488
HpyF10VI GCNNNNNNNGC 6 cut(s) 123, 132, 192, 241, 246, 375
HpyF3I CTNAG 1 cut(s) 506
HpySE526I ACGT 2 cut(s) 324, 488
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 6 cut(s) 142, 227, 265, 385, 391, 644
HspAI GCGC 1 cut(s) 35
Kpn2I TCCGGA 1 cut(s) 568
LpnPI CCDG 6 cut(s) 26, 61, 287, 311, 328, 582
Lsp1109I GCAGC 3 cut(s) 138, 170, 231
LweI GCATC 3 cut(s) 126, 261, 484
MaeI CTAG 1 cut(s) 548
MaeII ACGT 2 cut(s) 324, 488
MaeIII GTNAC 1 cut(s) 607
MbiI CCGCTC 1 cut(s) 235
MboII GAAGA 3 cut(s) 457, 460, 645
MhlI GDGCHC 1 cut(s) 213
MlyI GAGTC 3 cut(s) 92, 331, 424
MmeI TCCRAC 2 cut(s) 286, 386
MnlI CCTC 9 cut(s) 51, 100, 365, 369, 398, 465, 526, 547, 576
MroI TCCGGA 1 cut(s) 568
MseI TTAA 1 cut(s) 540
MslI CAYNNNNRTG 1 cut(s) 386
MspI CCGG 6 cut(s) 48, 274, 298, 315, 569, 658
MspR9I CCNGG 1 cut(s) 298
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 6 cut(s) 123, 132, 192, 241, 246, 375
NciI CCSGG 1 cut(s) 298
NlaIII CATG 6 cut(s) 142, 227, 265, 385, 391, 644
NlaIV GGNNCC 2 cut(s) 231, 344
NmeAIII GCCGAG 2 cut(s) 216, 225
NmuCI GTSAC 1 cut(s) 607
PagI TCATGA 3 cut(s) 138, 261, 387
PceI AGGCCT 1 cut(s) 546
PcsI WCGNNNNNNNCGW 3 cut(s) 174, 330, 494
PflFI GACNNNGTC 1 cut(s) 340
PflMI CCANNNNNTGG 1 cut(s) 300
PkrI GCNGC 6 cut(s) 128, 185, 221, 234, 251, 330
PleI GAGTC 3 cut(s) 92, 331, 423
PmaCI CACGTG 1 cut(s) 489
PmlI CACGTG 1 cut(s) 489
PpsI GAGTC 3 cut(s) 92, 331, 423
Ppu21I YACGTR 1 cut(s) 489
PspCI CACGTG 1 cut(s) 489
PspN4I GGNNCC 2 cut(s) 231, 344
PspPI GGNCC 2 cut(s) 291, 342
PsyI GACNNNGTC 1 cut(s) 340
RsaI GTAC 3 cut(s) 485, 513, 579
RsaNI GTAC 3 cut(s) 484, 512, 578
RseI CAYNNNNRTG 1 cut(s) 386
SaqAI TTAA 1 cut(s) 540
SatI GCNGC 6 cut(s) 127, 184, 220, 233, 250, 329
Sau96I GGNCC 2 cut(s) 291, 342
SchI GAGTC 3 cut(s) 92, 331, 424
ScrFI CCNGG 1 cut(s) 298
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 3 cut(s) 126, 261, 484
SfcI CTRYAG 2 cut(s) 130, 462
SinI GGWCC 2 cut(s) 291, 342
SmiMI CAYNNNNRTG 1 cut(s) 386
SseBI AGGCCT 1 cut(s) 546
SsiI CCGC 6 cut(s) 233, 249, 328, 367, 376, 458
SspMI CTAG 1 cut(s) 548
StuI AGGCCT 1 cut(s) 546
StyD4I CCNGG 1 cut(s) 296
TaaI ACNGT 3 cut(s) 178, 394, 593
TaiI ACGT 2 cut(s) 327, 491
TaqI TCGA 4 cut(s) 84, 120, 396, 585
TaqII GACCGA 1 cut(s) 330
TauI GCSGC 3 cut(s) 235, 252, 331
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TseFI GTSAC 1 cut(s) 607
TseI GCWGC 3 cut(s) 126, 183, 219
Tsp45I GTSAC 1 cut(s) 607
TspDTI ATGAA 5 cut(s) 127, 155, 178, 250, 657
Tth111I GACNNNGTC 1 cut(s) 340
Van91I CCANNNNNTGG 1 cut(s) 300
VpaK11BI GGWCC 2 cut(s) 291, 342
XagI CCTNNNNNAGG 1 cut(s) 551
XspI CTAG 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.