Rroxscaffold_6G00391490

Methionyl-trna

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0017862
Physical Location & Seq
Reverse (-)
9565874 .. 9570082
4209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00391490.1

Sequence Viewer

Length: 927 bp
ATGGGCCATCGCCGGAGCTGGAAGCTCCCCAACTCTTCAATCACCTACAACGTCTTCCACCGCTTCTATGGCCCGGTCAAGTTCATAGCAGCTGTCCAATCCATCAAGGTTTCGTTATGGCCGGTATTGGAGTGCGAATTTGATCCAAGGAATGCGAGGGGTGGCCTTGTCTTTGATGGTGTTTTTTTTGGGGGGGGGGGGGCGTCGACCGGGATCCTGGGGTTCTCGCAACCCGGTTTCGGAGCCGGGTTTTGGATGGGGCCACCGATGGGCGGAAGAATTGGAAGATTTTGGCATTACTCCTTCAGCCATGCTAATTGCAGTGCTTCTGGGATCGAATTGTGTAGTTGTGTGAATGAGGACCCAAGTGGGGTGTTGGGATTGAGGATTGTGAATGTGTTTCCTCGTGATTCCCGTATTCATTGTTCTGTACAATATAAATACAAGGTCCCTGTGACCTCTCTGATACGAAAGCATACATTTATTTTCGCGCTTACACAGCTTCCTTCCATATTTGATGGTTCAGCTAAATTAAAAGCGCAGTCCCAGGATGACTCTAAAGCTACATTAGCTCCAAAGATTTCTCCTGAGGAGTCATGGCTATCATTTGATCAGGAAGCTGTAGTCCTACATAACAAGGTTCGTGCATTTGCAGGATGGCCTGGAACTCGAGCTAAAGTTGTAGTTGTTGACAACAAAAACGGTCACCGCAATATGCTGGAGCTTAAAATTATCACAACCAAAGTTTGCAGCCAAAGCAATATTCAGGTCAACCAAGCAGATGACATCTCTTTTCGAAAGGGTGCATTAGTATTCCCCTGTGGCGGGTCCACATCACTTGAGGTGCTGGAGATTCAGCTTCCTGGTAAGAAGGTTATGAATGCAGCTGCCTTCTGGCCGGAACGGTTTACGTGGTCAACAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko00970 map00670 map00970
Pfam Domains
Protein Families
Formyl_trans_C Formyl_trans_N

Protein Analysis

308

Amino Acids

33.94

Weight (kDa)

9.51

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Formyl_trans_C PF02911 193 - 299 6.1e-16 Formyl transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 206
AccII CGCG 1 cut(s) 491
AciI CCGC 4 cut(s) 61, 273, 709, 825
AclWI GGATC 4 cut(s) 137, 208, 221, 341
AcoI YGGCCR 2 cut(s) 119, 896
AcsI RAATTY 1 cut(s) 137
AcuI CTGAAG 1 cut(s) 289
AcyI GRCGYC 1 cut(s) 203
AfaI GTAC 1 cut(s) 432
AfiI CCNNNNNNNGG 7 cut(s) 239, 252, 269, 272, 370, 824, 825
AgsI TTSAA 1 cut(s) 39
AjnI CCWGG 4 cut(s) 216, 546, 661, 862
AjuI GAANNNNNNNTTGG 2 cut(s) 747, 779
AlwI GGATC 4 cut(s) 137, 208, 221, 341
Ama87I CYCGRG 1 cut(s) 669
AoxI GGCC 7 cut(s) 4, 70, 119, 163, 260, 659, 896
ApeKI GCWGC 4 cut(s) 89, 750, 884, 887
ApoI RAATTY 1 cut(s) 137
AspLEI GCGC 2 cut(s) 493, 541
AspS9I GGNCC 6 cut(s) 4, 71, 260, 361, 448, 828
AsuC2I CCSGG 4 cut(s) 74, 211, 234, 247
AsuHPI GGTGA 2 cut(s) 34, 698
AsuII TTCGAA 1 cut(s) 796
AvaI CYCGRG 1 cut(s) 669
AvaII GGWCC 3 cut(s) 361, 448, 828
AxyI CCTNAGG 1 cut(s) 588
BamHI GGATCC 1 cut(s) 213
BauI CACGAG 1 cut(s) 405
BbsI GAAGAC 1 cut(s) 46
BbvI GCAGC 4 cut(s) 101, 762, 874, 896
BccI CCATC 7 cut(s) 15, 110, 170, 250, 262, 512, 651
BciT130I CCWGG 4 cut(s) 218, 548, 663, 864
BclI TGATCA 1 cut(s) 610
BcnI CCSGG 4 cut(s) 74, 211, 234, 247
BfmI CTRYAG 1 cut(s) 621
BisI GCNGC 4 cut(s) 90, 751, 885, 888
BlsI GCNGC 4 cut(s) 91, 752, 886, 889
Bme1390I CCNGG 8 cut(s) 74, 211, 218, 234, 247, 548, 663, 864
Bme18I GGWCC 3 cut(s) 361, 448, 828
BmeT110I CYCGRG 1 cut(s) 669
BmgT120I GGNCC 6 cut(s) 4, 71, 260, 361, 448, 828
BmiI GGNNCC 6 cut(s) 215, 244, 261, 363, 450, 829
BmrFI CCNGG 8 cut(s) 74, 211, 218, 234, 247, 548, 663, 864
BpiI GAAGAC 1 cut(s) 46
BpmI CTGGAG 2 cut(s) 740, 869
Bpu14I TTCGAA 1 cut(s) 796
BpuEI CTTGAG 1 cut(s) 860
BpuMI CCSGG 4 cut(s) 74, 211, 234, 247
BsaAI YACGTR 1 cut(s) 912
BsaHI GRCGYC 1 cut(s) 203
BsaJI CCNNGG 3 cut(s) 146, 217, 546
BsaXI ACNNNNNCTCC 2 cut(s) 556, 586
Bsc4I CCNNNNNNNGG 7 cut(s) 239, 252, 269, 272, 370, 824, 825
Bse118I RCCGGY 1 cut(s) 121
Bse21I CCTNAGG 1 cut(s) 588
BseBI CCWGG 4 cut(s) 218, 548, 663, 864
BseDI CCNNGG 3 cut(s) 146, 217, 546
BseGI GGATG 3 cut(s) 261, 556, 662
BseLI CCNNNNNNNGG 7 cut(s) 239, 252, 269, 272, 370, 824, 825
BseMII CTCAG 1 cut(s) 579
BseRI GAGGAG 1 cut(s) 605
BseXI GCAGC 4 cut(s) 101, 762, 874, 896
Bsh1236I CGCG 1 cut(s) 491
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 7 cut(s) 6, 72, 121, 165, 262, 661, 898
BsiEI CGRYCG 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 669
BsiSI CCGG 7 cut(s) 13, 74, 122, 210, 234, 246, 899
BslFI GGGAC 2 cut(s) 434, 529
BslI CCNNNNNNNGG 7 cut(s) 239, 252, 269, 272, 370, 824, 825
BsmFI GGGAC 2 cut(s) 434, 529
BsmI GAATGC 2 cut(s) 157, 886
BsnI GGCC 7 cut(s) 6, 72, 121, 165, 262, 661, 898
BsoBI CYCGRG 1 cut(s) 669
Bsp119I TTCGAA 1 cut(s) 796
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 4 cut(s) 142, 213, 333, 610
BspACI CCGC 4 cut(s) 61, 273, 709, 825
BspANI GGCC 7 cut(s) 6, 72, 121, 165, 262, 661, 898
BspCNI CTCAG 1 cut(s) 580
BspFNI CGCG 1 cut(s) 491
BspLI GGNNCC 6 cut(s) 215, 244, 261, 363, 450, 829
BspPI GGATC 4 cut(s) 137, 208, 221, 341
BspT104I TTCGAA 1 cut(s) 796
BsrFI RCCGGY 1 cut(s) 121
BsrGI TGTACA 1 cut(s) 430
BssAI RCCGGY 1 cut(s) 121
BssECI CCNNGG 3 cut(s) 146, 217, 546
BssMI GATC 4 cut(s) 142, 213, 333, 610
BssNI GRCGYC 1 cut(s) 203
BssSI CACGAG 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 405
Bst2UI CCWGG 4 cut(s) 218, 548, 663, 864
Bst4CI ACNGT 2 cut(s) 704, 906
Bst6I CTCTTC 1 cut(s) 40
BstACI GRCGYC 1 cut(s) 203
BstAUI TGTACA 1 cut(s) 430
BstBAI YACGTR 1 cut(s) 912
BstBI TTCGAA 1 cut(s) 796
BstDEI CTNAG 1 cut(s) 588
BstEII GGTNACC 1 cut(s) 704
BstF5I GGATG 3 cut(s) 261, 556, 662
BstFNI CGCG 1 cut(s) 491
BstHHI GCGC 2 cut(s) 493, 541
BstKTI GATC 4 cut(s) 145, 216, 336, 613
BstMBI GATC 4 cut(s) 142, 213, 333, 610
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 4 cut(s) 69, 499, 569, 756
BstNI CCWGG 4 cut(s) 218, 548, 663, 864
BstPI GGTNACC 1 cut(s) 704
BstSCI CCNGG 8 cut(s) 72, 209, 216, 232, 245, 546, 661, 862
BstSFI CTRYAG 1 cut(s) 621
BstUI CGCG 1 cut(s) 491
BstV1I GCAGC 4 cut(s) 101, 762, 874, 896
BstV2I GAAGAC 1 cut(s) 46
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
Bsu36I CCTNAGG 1 cut(s) 588
BsuRI GGCC 7 cut(s) 6, 72, 121, 165, 262, 661, 898
BtsCI GGATG 3 cut(s) 261, 556, 662
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 1 cut(s) 328
CfoI GCGC 2 cut(s) 493, 541
Cfr10I RCCGGY 1 cut(s) 121
Cfr13I GGNCC 6 cut(s) 4, 71, 260, 361, 448, 828
CseI GACGC 1 cut(s) 192
Csp6I GTAC 1 cut(s) 431
CspCI CAANNNNNGTGG 2 cut(s) 820, 855
CviAII CATG 2 cut(s) 311, 597
CviQI GTAC 1 cut(s) 431
DdeI CTNAG 1 cut(s) 588
DpnI GATC 4 cut(s) 144, 215, 335, 612
DpnII GATC 4 cut(s) 142, 213, 333, 610
EaeI YGGCCR 2 cut(s) 119, 896
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
EciI GGCGGA 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 146
Eco47I GGWCC 3 cut(s) 361, 448, 828
Eco57I CTGAAG 1 cut(s) 289
Eco81I CCTNAGG 1 cut(s) 588
Eco88I CYCGRG 1 cut(s) 669
Eco91I GGTNACC 1 cut(s) 704
EcoO109I RGGNCCY 2 cut(s) 361, 448
EcoO65I GGTNACC 1 cut(s) 704
EcoRII CCWGG 4 cut(s) 216, 546, 661, 862
EcoT14I CCWWGG 1 cut(s) 146
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 2 cut(s) 314, 600
FaqI GGGAC 2 cut(s) 434, 529
FatI CATG 2 cut(s) 310, 596
FauI CCCGC 1 cut(s) 818
FbaI TGATCA 1 cut(s) 610
FblI GTMKAC 1 cut(s) 206
Fnu4HI GCNGC 4 cut(s) 90, 751, 885, 888
FokI GGATG 3 cut(s) 268, 563, 669
Fsp4HI GCNGC 4 cut(s) 90, 751, 885, 888
GlaI GCGC 2 cut(s) 492, 540
GluI GCNGC 4 cut(s) 90, 751, 885, 888
GsuI CTGGAG 2 cut(s) 740, 869
HaeIII GGCC 7 cut(s) 6, 72, 121, 165, 262, 661, 898
HapII CCGG 7 cut(s) 13, 74, 122, 210, 234, 246, 899
HgaI GACGC 1 cut(s) 192
HhaI GCGC 2 cut(s) 493, 541
Hin1I GRCGYC 1 cut(s) 203
Hin1II CATG 2 cut(s) 314, 600
Hin6I GCGC 2 cut(s) 491, 539
HinP1I GCGC 2 cut(s) 491, 539
HincII GTYRAC 4 cut(s) 207, 691, 772, 918
HindII GTYRAC 4 cut(s) 207, 691, 772, 918
HinfI GANTC 4 cut(s) 410, 554, 593, 853
HpaII CCGG 7 cut(s) 13, 74, 122, 210, 234, 246, 899
HphI GGTGA 2 cut(s) 34, 698
Hpy166II GTNNAC 6 cut(s) 207, 691, 772, 831, 909, 918
Hpy188I TCNGA 2 cut(s) 242, 465
Hpy188III TCNNGA 3 cut(s) 407, 587, 614
Hpy8I GTNNAC 6 cut(s) 207, 691, 772, 831, 909, 918
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 4 cut(s) 313, 516, 865, 901
HpyCH4III ACNGT 2 cut(s) 704, 906
HpyCH4IV ACGT 2 cut(s) 51, 911
HpyCH4V TGCA 6 cut(s) 321, 647, 653, 750, 806, 884
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 499, 569, 756
HpyF3I CTNAG 1 cut(s) 588
HpySE526I ACGT 2 cut(s) 51, 911
Hsp92I GRCGYC 1 cut(s) 203
Hsp92II CATG 2 cut(s) 314, 600
HspAI GCGC 2 cut(s) 491, 539
Ksp22I TGATCA 1 cut(s) 610
Kzo9I GATC 4 cut(s) 142, 213, 333, 610
LmnI GCTCC 5 cut(s) 15, 30, 242, 577, 721
Lsp1109I GCAGC 4 cut(s) 101, 762, 874, 896
MaeII ACGT 2 cut(s) 51, 911
MaeIII GTNAC 2 cut(s) 454, 704
MalI GATC 4 cut(s) 144, 215, 335, 612
MboI GATC 4 cut(s) 142, 213, 333, 610
MboII GAAGA 4 cut(s) 27, 46, 288, 297
MflI RGATCY 1 cut(s) 213
MluCI AATT 6 cut(s) 137, 279, 316, 338, 530, 729
MlyI GAGTC 2 cut(s) 548, 602
MnlI CCTC 7 cut(s) 150, 352, 378, 414, 469, 583, 835
MseI TTAA 3 cut(s) 533, 726, 925
MspA1I CMGCKG 2 cut(s) 92, 887
MspI CCGG 7 cut(s) 13, 74, 122, 210, 234, 246, 899
MspR9I CCNGG 8 cut(s) 74, 211, 218, 234, 247, 548, 663, 864
Mva1269I GAATGC 2 cut(s) 157, 886
MvaI CCWGG 4 cut(s) 218, 548, 663, 864
MvnI CGCG 1 cut(s) 491
MwoI GCNNNNNNNGC 4 cut(s) 69, 499, 569, 756
NciI CCSGG 4 cut(s) 74, 211, 234, 247
NdeII GATC 4 cut(s) 142, 213, 333, 610
NlaIII CATG 2 cut(s) 314, 600
NlaIV GGNNCC 6 cut(s) 215, 244, 261, 363, 450, 829
NmuCI GTSAC 2 cut(s) 454, 704
NspV TTCGAA 1 cut(s) 796
PaeR7I CTCGAG 1 cut(s) 669
PcsI WCGNNNNNNNCGW 1 cut(s) 412
PctI GAATGC 2 cut(s) 157, 886
PfeI GAWTC 2 cut(s) 410, 853
PkrI GCNGC 4 cut(s) 91, 752, 886, 889
PleI GAGTC 2 cut(s) 548, 601
PpsI GAGTC 2 cut(s) 548, 601
Ppu21I YACGTR 1 cut(s) 912
PpuMI RGGWCCY 2 cut(s) 361, 448
Psp5II RGGWCCY 2 cut(s) 361, 448
Psp6I CCWGG 4 cut(s) 216, 546, 661, 862
PspEI GGTNACC 1 cut(s) 704
PspGI CCWGG 4 cut(s) 216, 546, 661, 862
PspN4I GGNNCC 6 cut(s) 215, 244, 261, 363, 450, 829
PspPI GGNCC 6 cut(s) 4, 71, 260, 361, 448, 828
PspPPI RGGWCCY 2 cut(s) 361, 448
PspXI VCTCGAGB 1 cut(s) 669
PsrI GAACNNNNNNTAC 2 cut(s) 409, 441
PsuI RGATCY 1 cut(s) 213
PvuII CAGCTG 2 cut(s) 92, 887
RsaI GTAC 1 cut(s) 432
RsaNI GTAC 1 cut(s) 431
SalI GTCGAC 1 cut(s) 205
SaqAI TTAA 3 cut(s) 533, 726, 925
SatI GCNGC 4 cut(s) 90, 751, 885, 888
Sau3AI GATC 4 cut(s) 142, 213, 333, 610
Sau96I GGNCC 6 cut(s) 4, 71, 260, 361, 448, 828
SchI GAGTC 2 cut(s) 548, 602
ScrFI CCNGG 8 cut(s) 74, 211, 218, 234, 247, 548, 663, 864
SfcI CTRYAG 1 cut(s) 621
Sfr274I CTCGAG 1 cut(s) 669
SfuI TTCGAA 1 cut(s) 796
SinI GGWCC 3 cut(s) 361, 448, 828
SlaI CTCGAG 1 cut(s) 669
SmlI CTYRAG 2 cut(s) 669, 839
SmoI CTYRAG 2 cut(s) 669, 839
Sse9I AATT 6 cut(s) 137, 279, 316, 338, 530, 729
SsiI CCGC 4 cut(s) 61, 273, 709, 825
SspI AATATT 1 cut(s) 763
StyD4I CCNGG 8 cut(s) 72, 209, 216, 232, 245, 546, 661, 862
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 2 cut(s) 704, 906
TaiI ACGT 2 cut(s) 54, 914
TaqI TCGA 4 cut(s) 206, 336, 670, 796
TasI AATT 6 cut(s) 137, 279, 316, 338, 530, 729
TatI WGTACW 1 cut(s) 430
TfiI GAWTC 2 cut(s) 410, 853
Tru1I TTAA 3 cut(s) 533, 726, 925
Tru9I TTAA 3 cut(s) 533, 726, 925
TscAI CASTG 1 cut(s) 328
TseFI GTSAC 2 cut(s) 454, 704
TseI GCWGC 4 cut(s) 89, 750, 884, 887
Tsp45I GTSAC 2 cut(s) 454, 704
TspDTI ATGAA 3 cut(s) 73, 410, 893
TspRI CASTG 1 cut(s) 328
VpaK11BI GGWCC 3 cut(s) 361, 448, 828
XapI RAATTY 1 cut(s) 137
XcmI CCANNNNNNNNNTGG 1 cut(s) 65
XhoI CTCGAG 1 cut(s) 669
XmiI GTMKAC 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.