Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002113

Physical Location & Seq

Genomic Coordinates Forward (+)

33981253 .. 33992507

Gene Structure 11255 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00241740.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3420 bp

ATGCCTCCTCCTCATTTTGTGAGCGGATGGATGTATGTTAATGAACAAGGACAAATGTGCGGCCCCTATATTCAGCAGCAGTTATTTGAAGGTTTATCTACTGGTTTTTTGCCTGATGAGCTTCCTGTATATCCTGTGGTAAATGGGGCATTAATAAACCCAATACCATTGAAGTATTTCAAGCAGTTCCCTGATCATGTTGCTACTGGGTTTGCATATCTGAGTCTGGGCAGCATCTCGAGCACAAGCACGCCTACAAATTCTCTTAAATCTTGCAATGGGGATTTGGCTACATGTACTATACCTACACCGGTTGCTACCGGTTATCCTGATTTACACACCTATCAAGTTACTCAAATCAGTCTACTGTCAAGTAAAGATTCTTGCTGGTTGTATGAGGATGAGGAAGGGAAGAGAAATGGGCCATATTCTCTTTTCGAACTAAATTCTTGGCATCGATATGGATATCTTCGGGATTCGCTGACGATTTATCATGTCAAGAACAAGTGTAAACCCTTCACACTGTCATCGGTTCTCAATTCATGGAAACTTGATGGACCTGAAACCATCACCAAATTTGATGCCAAATGTAATGAGTCTGGCCCATTGGTAAGCATTATATCTGAAGTCACAGAGGGAGTTTCTTCCCAACTGCACTACGGAATTATGAAATCAGCTCGTAGAGTTGTTTTAGATGAGATAATCAGCAATGTGATTGCTGAGTTTGTTACCACCACGAAAGCTCAGAGACTTAACCAGGATATGAAGACCTGGTCCTTGGATGCAAAAAAGTCTGAAATTGACGGTGAGGCTGGAGTTTCCGACTATGTTGCTGAAAGGACATGTATCAATCAGGATTCACCTTCACCAATTACCAAATCTGTTGGAAGCATTCAGAACTTTTGGGATTCGTATGCAGTTGTTTGTAGAATGCTCTTTGATTATTGCATGGAAGTCATGTGGAATGCTGTCTTCTATGATTCAGTGGCAGAATATTCATCTGCTTGGAGGAGAAGGAAACTATGGACTGGCTCTCCATTGTTGTGGAGACCTCCTAATAGATGTGGTGACTGGGTGGAAAAGATCACAAAATTACCTCATGAAATTTTATCTGATAATGATGATGATGATCGTCCCCCTGGTTTTGAACTGTTGGGAGCTGAGTTAGGGCGTCATGCTCAGCCATCATTGAGCTCTTCATTGGTTCTAATGGAGGAAAAACCACCTAAACAGATAGGTCCCTCATACGAAGACATGAAGTACATTGTAGAGCATATAGAAAATGAACTTCAATTGTCTGCAAAGAGTTCCTTGACCGAGTATGTTGGATACTTTGTTGATGAGGAAGTGAGTAAACTAGTTACTTCGTCCACAGAAGAGAATTCAATAAAGGAGAATATTGACTGTTCCCCGAGGAGCATTGGTGGCTCATCTGACCTATGTGACAATTTGAGGACTTCAAATAACACATCTGCGGAAATTATTCTGTCTGAGATATCCCTAACTCCAGAAGTAGAAAAACCCTTCCATAATTCTTTTCCTGAAAATCGTATCTCTAATTTTCTGGCAAGTGCTTTTAAGGAAGTCTGTTCTCATGTTGATGATCTTGATGGTGACCAAGAAGTAAATGAACCATCACCCCCTGGACTCATAGCCAATGTTAAAACTCTTGGACAGTCACCTATTTGCAAATTCCGACCTTCAAGGTCAGAAGAATGTATTCCTAAGATTGGAGCATGTGTTGCTACAGCAATGTTGCGGAAGAAACTCCATGATGATGTTATTGGAGAATGGAAATCATCATTTATTGATCTGGCTCTTAATAAGTTTCTTGCACCATGGCGAACTTCAAGGAAGAACCATATCCGTAATGAGGAAAAAGCATGCAACACCAATAAAGAAGAATTAAAGCATTGTCACAGTTCTATCACTGCAGAAGTATCTCCAGTGATCGATGAATATACATATCAGCGCAAGAGGTTGCGGAAAAAGTCAGGTTCTTCTGGACTTGTAACTGTAATTGATACTGGGTTAAAGAGTGAAACAGTGGAAAAGGCAAAGAAGCTACGTGTTGCTAGAAATGTGCCGAAGAATGCTACTGTGATTCCTAGAAACAGAGGTCTAAGCAAACCCCAAACTGAATCATCCATTGATGCCATATCTGTGAAAGTTAATTCTAAAAGGCTTTCATCAACTGATAAGTCAGCAGCTAAGAATGCTAGTAGTCGGAAGCCATTGAAGGGTTCTCACGAAGAAAGTTGTGAGCCCATTGACGATGTTATTGATGGCATCAGCTCAGTTAGGGGATCCCAAACTGAATTATCTGTTGGTGCCATTGCTTTGCAAGCCCAATATAAGAGGTGTTCATCCACTGATACATTGGTAGCTAATAATGCTATTAGTCGGAAACGATTGAAGGTTTCTCATGCAGTTAAAGGTAGTGAATCCATGGAGTGCACTCCTAAACCTAGTAAAAGGATGGTTTCAGCTCATGTCCGGGATCACAGTGATATTGAGAATGTTGTAAATAGCCATGGTCGTGATGATCGGCTTAAAGGAGAGCCTTTAACTAAAGTGTTGAAGCTGAAAAGGGATCGTCCAGTTGGCAGTTCACAATTGCCACATCCCAATAAGGTCTTGAAAGTAGCCAATGATGTTCTCAAGCAAGCAGCAAATATCCCAGTTGCAGTAAGAAAGGCCCGGTCCAGTAAGTCAAAGACATCCAATCCTTGCCCCAAATCTGATGGATGTGCCCGTGCTTCAATTAATGGCTGGGAATGGCATAGATGGTCACTAAATGCAACTCCTGCTGAAAGAGCTCGTGGTAGGGGAATCAAATATGTTAATGCCCAGTATCGGACTTCTGACACTAATGCATCACAGTGGTCAAATGGCAAGGGTCTTTCTGCAAGAACAAATAGGGTGAAGATGCGTAGTCTTCTTGCTGCTGCTGAGGGTGCTGACCTTTTGAAAGCCACTCAATTAAAGGCAAGGAAAAAGCGTCTACGTTTCCAGCGGAGCAAGATACATGATTGGGGTCTTATTGCCTTGGAGCCAATCGAGGCAGAAGACTTTGTTATTGAATATGTTGGAGAATTGATTCGTCCCCAGATATCTGATATACGCGAGCGTCATTACGAGAAGATGGGAATTGGCAGCAGTTATTTGTTTAGGATTGATGATGGTTATGTGGTTGATGCTACAAAACGTGGAGGAATTGCTAGATTCATAAACCATTCTTGTGAGCCTAACTGCTACACCAAGGTTATAAGTGTTGAGGGTGAGAAAAAGATTTTCATTTATGCAAAACGACATATAGCTGCTGGTGAAGAAATTACTTACAATTATAAGTTCCCATTAGAGGAGAAAAAGATTCCATGCAATTGTGGTTCAAAAAGGTGCCGTGGATCATTGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006325 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009791 GO:0009909 GO:0009987 GO:0010228 GO:0010452 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018193 GO:0018205 GO:0019538 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0034968 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048580 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0051276 GO:0051568 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko00310 map00310

Pfam Domains

Protein Families

SET

Protein Analysis

1139

Amino Acids

127.13

Weight (kDa)

8.76

Isoelectric Point (pI)

46.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GYF	PF02213	130 - 168	7e-06	GYF domain
SET	PF00856	1012 - 1117	1.1e-27	SET domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42400 AT5G42400 AT5G42400 AT5G42400 AT5G42400 AT5G42400 AT5G42400 AT5G42400
fragaria_vesca	FvH4_5g26810 FvH4_5g26810 FvH4_5g26810 FvH4_5g26810 FvH4_5g26810 FvH4_5g26810 FvH4_5g26810 FvH4_5g26810 FvH4_5g26810 FvH4_5g26810 FvH4_5g26810 FvH4_5g26810 FvH4_5g26810 FvH4_5g26810 FvH4_5g26810
malus_domestica	MD02G1278400.v1.1 MD07G1046400.v1.1 MD07G1046500.v1.1
prunus_persica	Prupe.2G042400_v2.0.a1 Prupe.2G042400_v2.0.a1 Prupe.2G042400_v2.0.a1 Prupe.2G042400_v2.0.a1 Prupe.2G042400_v2.0.a1
pyrus_communis	pycom02g23860
rosa_chinensis	RchiOBHm_Chr7g0217731
rosa_laevigata	RLG00000002444
rosa_multiflora	Rmu_co8471317.1_g000001 Rmu_sc0000202.1_g000011
rosa_roxburghii	Rroxscaffold_3G00241740
rosa_rugosa	Rorug07G0170800 Rorug07G0170800
rosa_samantha	Rh7AG311300 Rh7BG303200 Rh7CG330300 Rh7DG311100
rosa_wichuraiana	Rw7G026430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	3269, 3348
AccB1I	GGYRCC	2 cut(s)	2330, 3399
AccBSI	CCGCTC	1 cut(s)	24
AccI	GTMKAC	2 cut(s)	364, 3004
AccII	CGCG	1 cut(s)	3126
AciI	CCGC	6 cut(s)	24, 60, 1475, 1759, 1984, 3016
AclWI	GGATC	5 cut(s)	2301, 2314, 2508, 2601, 3415
AcsI	RAATTY	6 cut(s)	259, 445, 575, 1104, 1381, 1691
AcuI	CTGAAG	1 cut(s)	645
AcyI	GRCGYC	1 cut(s)	1171
AfaI	GTAC	2 cut(s)	298, 1262
AfiI	CCNNNNNNNGG	5 cut(s)	1730, 1873, 2240, 2856, 3361
AflIII	ACRYGT	3 cut(s)	293, 842, 2068
AgeI	ACCGGT	2 cut(s)	310, 320
AhlI	ACTAGT	1 cut(s)	1357
AjnI	CCWGG	4 cut(s)	756, 770, 1138, 1642
AjuI	GAANNNNNNNTTGG	2 cut(s)	2310, 2342
Alw21I	GWGCWC	4 cut(s)	245, 1196, 2459, 2821
Alw26I	GTCTC	2 cut(s)	742, 1042
Alw44I	GTGCAC	1 cut(s)	2455
AlwI	GGATC	5 cut(s)	2301, 2314, 2508, 2601, 3415
Ama87I	CYCGRG	2 cut(s)	238, 1411
AoxI	GGCC	4 cut(s)	61, 422, 601, 2697
ApaLI	GTGCAC	1 cut(s)	2455
ApeKI	GCWGC	8 cut(s)	76, 231, 2207, 2669, 2945, 2948, 3156, 3320
ApoI	RAATTY	6 cut(s)	259, 445, 575, 1104, 1381, 1691
AseI	ATTAAT	2 cut(s)	152, 2766
AsiGI	ACCGGT	2 cut(s)	310, 320
Asp700I	GAANNNNTTC	4 cut(s)	176, 1482, 1719, 3099
AspLEI	GCGC	1 cut(s)	1974
AspS9I	GGNCC	8 cut(s)	62, 422, 557, 602, 774, 1238, 2698, 2703
AsuC2I	CCSGG	2 cut(s)	2498, 2701
AsuII	TTCGAA	1 cut(s)	438
AvaI	CYCGRG	2 cut(s)	238, 1411
AvaII	GGWCC	4 cut(s)	557, 774, 1238, 2703
BaeGI	GKGCMC	2 cut(s)	2459, 2755
BaeI	ACNNNNGTAYC	2 cut(s)	2367, 2400
BamHI	GGATCC	1 cut(s)	2306
BanI	GGYRCC	2 cut(s)	2330, 3399
BanII	GRGCYC	3 cut(s)	1196, 2268, 2821
BauI	CACGAG	1 cut(s)	2820
BbsI	GAAGAC	5 cut(s)	773, 964, 1257, 2930, 3075
Bbv12I	GWGCWC	4 cut(s)	245, 1196, 2459, 2821
BbvCI	CCTCAGC	1 cut(s)	2952
BbvI	GCAGC	8 cut(s)	88, 243, 2219, 2681, 2932, 2935, 3168, 3307
BceAI	ACGGC	1 cut(s)	3387
BcgI	CGANNNNNNTGC	2 cut(s)	812, 846
BciT130I	CCWGG	4 cut(s)	758, 772, 1140, 1644
BciVI	GTATCC	1 cut(s)	1322
BclI	TGATCA	1 cut(s)	193
BcnI	CCSGG	2 cut(s)	2498, 2701
BcoDI	GTCTC	2 cut(s)	742, 1042
BcuI	ACTAGT	1 cut(s)	1357
BfaI	CTAG	6 cut(s)	1358, 2076, 2109, 2220, 2469, 3222
BfmI	CTRYAG	2 cut(s)	1746, 1932
BfuI	GTATCC	1 cut(s)	1322
BisI	GCNGC	9 cut(s)	61, 77, 232, 2208, 2670, 2946, 2949, 3157, 3321
BlpI	GCTNAGC	1 cut(s)	1179
BlsI	GCNGC	9 cut(s)	62, 78, 233, 2209, 2671, 2947, 2950, 3158, 3322
Bme1390I	CCNGG	6 cut(s)	758, 772, 1140, 1644, 2498, 2701
Bme18I	GGWCC	4 cut(s)	557, 774, 1238, 2703
BmeT110I	CYCGRG	2 cut(s)	238, 1411
BmgT120I	GGNCC	8 cut(s)	62, 422, 557, 602, 774, 1238, 2698, 2703
BmiI	GGNNCC	6 cut(s)	64, 1240, 2308, 2332, 3054, 3401
BmrFI	CCNGG	6 cut(s)	758, 772, 1140, 1644, 2498, 2701
BmrI	ACTGGG	5 cut(s)	216, 1081, 2037, 2675, 2845
BmsI	GCATC	9 cut(s)	243, 463, 571, 772, 2143, 2298, 2885, 2919, 3187
BmuI	ACTGGG	5 cut(s)	216, 1081, 2037, 2675, 2845
BpiI	GAAGAC	5 cut(s)	773, 964, 1257, 2930, 3075
BpmI	CTGGAG	3 cut(s)	834, 1491, 1929
Bpu10I	CCTNAGC	1 cut(s)	2952
Bpu1102I	GCTNAGC	1 cut(s)	1179
Bpu14I	TTCGAA	1 cut(s)	438
BpuEI	CTTGAG	1 cut(s)	2645
BpuMI	CCSGG	2 cut(s)	2498, 2701
Bsa29I	ATCGAT	2 cut(s)	457, 1953
BsaAI	YACGTR	1 cut(s)	2069
BsaBI	GATNNNNATC	1 cut(s)	1128
BsaHI	GRCGYC	1 cut(s)	1171
BsaI	GGTCTC	1 cut(s)	1042
BsaWI	WCCGGW	2 cut(s)	310, 320
BsaXI	ACNNNNNCTCC	6 cut(s)	1018, 1048, 1408, 1438, 1725, 1755
Bsc4I	CCNNNNNNNGG	5 cut(s)	1730, 1873, 2240, 2856, 3361
Bse118I	RCCGGY	2 cut(s)	310, 320
Bse3DI	GCAATG	4 cut(s)	283, 715, 1758, 2334
Bse8I	GATNNNNATC	1 cut(s)	1128
BseBI	CCWGG	4 cut(s)	758, 772, 1140, 1644
BseCI	ATCGAT	2 cut(s)	457, 1953
BseJI	GATNNNNATC	1 cut(s)	1128
BseLI	CCNNNNNNNGG	5 cut(s)	1730, 1873, 2240, 2856, 3361
BseMI	GCAATG	4 cut(s)	283, 715, 1758, 2334
BseMII	CTCAG	8 cut(s)	212, 711, 758, 1152, 1193, 1482, 2310, 2943
BseRI	GAGGAG	3 cut(s)	1024, 1429, 3377
BseSI	GKGCMC	2 cut(s)	2459, 2755
BseXI	GCAGC	8 cut(s)	88, 243, 2219, 2681, 2932, 2935, 3168, 3307
BseYI	CCCAGC	1 cut(s)	2772
BsgI	GTGCAG	1 cut(s)	638
Bsh1236I	CGCG	1 cut(s)	3126
BshFI	GGCC	4 cut(s)	63, 424, 603, 2699
BshNI	GGYRCC	2 cut(s)	2330, 3399
BshTI	ACCGGT	2 cut(s)	310, 320
BshVI	ATCGAT	2 cut(s)	457, 1953
BsiHKAI	GWGCWC	4 cut(s)	245, 1196, 2459, 2821
BsiHKCI	CYCGRG	2 cut(s)	238, 1411
BsiSI	CCGG	4 cut(s)	311, 321, 2497, 2701
BslFI	GGGAC	3 cut(s)	1119, 1224, 3090
BslI	CCNNNNNNNGG	5 cut(s)	1730, 1873, 2240, 2856, 3361
BsmAI	GTCTC	2 cut(s)	742, 1042
BsmFI	GGGAC	3 cut(s)	1119, 1224, 3090
BsmI	GAATGC	5 cut(s)	891, 936, 970, 2098, 2221
BsnI	GGCC	4 cut(s)	63, 424, 603, 2699
Bso31I	GGTCTC	1 cut(s)	1042
BsoBI	CYCGRG	2 cut(s)	238, 1411
Bsp119I	TTCGAA	1 cut(s)	438
Bsp1286I	GDGCHC	6 cut(s)	245, 1196, 2268, 2459, 2755, 2821
Bsp1720I	GCTNAGC	1 cut(s)	1179
Bsp19I	CCATGG	3 cut(s)	1838, 2448, 2533
BspACI	CCGC	6 cut(s)	24, 60, 1475, 1759, 1984, 3016
BspANI	GGCC	4 cut(s)	63, 424, 603, 2699
BspCNI	CTCAG	8 cut(s)	213, 712, 757, 1153, 1192, 1483, 2309, 2944
BspDI	ATCGAT	2 cut(s)	457, 1953
BspFNI	CGCG	1 cut(s)	3126
BspHI	TCATGA	1 cut(s)	1099
BspLI	GGNNCC	6 cut(s)	64, 1240, 2308, 2332, 3054, 3401
BspMAI	CTGCAG	1 cut(s)	1936
BspPI	GGATC	5 cut(s)	2301, 2314, 2508, 2601, 3415
BspQI	GCTCTTC	1 cut(s)	1201
BspT104I	TTCGAA	1 cut(s)	438
BspT107I	GGYRCC	2 cut(s)	2330, 3399
BspTNI	GGTCTC	1 cut(s)	1042
BsrBI	CCGCTC	1 cut(s)	24
BsrDI	GCAATG	4 cut(s)	283, 715, 1758, 2334
BsrFI	RCCGGY	2 cut(s)	310, 320
BssAI	RCCGGY	2 cut(s)	310, 320
BssNI	GRCGYC	1 cut(s)	1171
BssSI	CACGAG	1 cut(s)	2820
BssT1I	CCWWGG	6 cut(s)	777, 1838, 2448, 2533, 3048, 3261
Bst2BI	CACGAG	1 cut(s)	2820
Bst2UI	CCWGG	4 cut(s)	758, 772, 1140, 1644
Bst6I	CTCTTC	3 cut(s)	407, 1201, 1371
BstACI	GRCGYC	1 cut(s)	1171
BstAPI	GCANNNNNTGC	2 cut(s)	1742, 2807
BstBAI	YACGTR	1 cut(s)	2069
BstBI	TTCGAA	1 cut(s)	438
BstC8I	GCNNGC	5 cut(s)	251, 1885, 2346, 2667, 3128
BstDSI	CCRYGG	4 cut(s)	1838, 2448, 2533, 3403
BstEII	GGTNACC	1 cut(s)	1613
BstFNI	CGCG	1 cut(s)	3126
BstHHI	GCGC	1 cut(s)	1974
BstMAI	GTCTC	2 cut(s)	742, 1042
BstNI	CCWGG	4 cut(s)	758, 772, 1140, 1644
BstNSI	RCATGY	4 cut(s)	297, 846, 1740, 1887
BstPI	GGTNACC	1 cut(s)	1613
BstSCI	CCNGG	6 cut(s)	756, 770, 1138, 1642, 2496, 2699
BstSFI	CTRYAG	2 cut(s)	1746, 1932
BstSLI	GKGCMC	2 cut(s)	2459, 2755
BstUI	CGCG	1 cut(s)	3126
BstV1I	GCAGC	8 cut(s)	88, 243, 2219, 2681, 2932, 2935, 3168, 3307
BstV2I	GAAGAC	5 cut(s)	773, 964, 1257, 2930, 3075
BstX2I	RGATCY	1 cut(s)	2306
BstXI	CCANNNNNNTGG	1 cut(s)	1044
BstYI	RGATCY	1 cut(s)	2306
Bsu15I	ATCGAT	2 cut(s)	457, 1953
BsuI	GTATCC	1 cut(s)	1322
BsuRI	GGCC	4 cut(s)	63, 424, 603, 2699
BsuTUI	ATCGAT	2 cut(s)	457, 1953
BtgI	CCRYGG	4 cut(s)	1838, 2448, 2533, 3403
BtsI	GCAGTG	1 cut(s)	1929
BtsIMutI	CAGTG	8 cut(s)	521, 990, 1929, 1953, 2052, 2370, 2512, 2888
Cac8I	GCNNGC	5 cut(s)	251, 1885, 2346, 2667, 3128
CciI	TCATGA	1 cut(s)	1099
CfoI	GCGC	1 cut(s)	1974
Cfr10I	RCCGGY	2 cut(s)	310, 320
Cfr13I	GGNCC	8 cut(s)	62, 422, 557, 602, 774, 1238, 2698, 2703
ClaI	ATCGAT	2 cut(s)	457, 1953
CseI	GACGC	3 cut(s)	1160, 2990, 3119
CsiI	ACCWGGT	1 cut(s)	770
Csp6I	GTAC	2 cut(s)	297, 1261
CspAI	ACCGGT	2 cut(s)	310, 320
CviQI	GTAC	2 cut(s)	297, 1261
Eam1104I	CTCTTC	3 cut(s)	407, 1201, 1371
EarI	CTCTTC	3 cut(s)	407, 1201, 1371
Ecl136II	GAGCTC	2 cut(s)	1194, 2819
Eco130I	CCWWGG	6 cut(s)	777, 1838, 2448, 2533, 3048, 3261
Eco24I	GRGCYC	3 cut(s)	1196, 2268, 2821
Eco31I	GGTCTC	1 cut(s)	1042
Eco32I	GATATC	3 cut(s)	467, 1497, 3114
Eco47I	GGWCC	4 cut(s)	557, 774, 1238, 2703
Eco53kI	GAGCTC	2 cut(s)	1194, 2819
Eco57I	CTGAAG	1 cut(s)	645
Eco88I	CYCGRG	2 cut(s)	238, 1411
Eco91I	GGTNACC	1 cut(s)	1613
EcoICRI	GAGCTC	2 cut(s)	1194, 2819
EcoO109I	RGGNCCY	1 cut(s)	1238
EcoO65I	GGTNACC	1 cut(s)	1613
EcoRI	GAATTC	1 cut(s)	1381
EcoRII	CCWGG	4 cut(s)	756, 770, 1138, 1642
EcoRV	GATATC	3 cut(s)	467, 1497, 3114
EcoT14I	CCWWGG	6 cut(s)	777, 1838, 2448, 2533, 3048, 3261
EcoT22I	ATGCAT	1 cut(s)	2878
EcoT38I	GRGCYC	3 cut(s)	1196, 2268, 2821
ErhI	CCWWGG	6 cut(s)	777, 1838, 2448, 2533, 3048, 3261
FalI	AAGNNNNNCTT	2 cut(s)	1295, 1327
FaqI	GGGAC	3 cut(s)	1119, 1224, 3090
FbaI	TGATCA	1 cut(s)	193
FblI	GTMKAC	2 cut(s)	364, 3004
Fnu4HI	GCNGC	9 cut(s)	61, 77, 232, 2208, 2670, 2946, 2949, 3157, 3321
FriOI	GRGCYC	3 cut(s)	1196, 2268, 2821
Fsp4HI	GCNGC	9 cut(s)	61, 77, 232, 2208, 2670, 2946, 2949, 3157, 3321
FspBI	CTAG	6 cut(s)	1358, 2076, 2109, 2220, 2469, 3222
GlaI	GCGC	1 cut(s)	1973
GluI	GCNGC	9 cut(s)	61, 77, 232, 2208, 2670, 2946, 2949, 3157, 3321
GsaI	CCCAGC	1 cut(s)	2776
GsuI	CTGGAG	3 cut(s)	834, 1491, 1929
HaeIII	GGCC	4 cut(s)	63, 424, 603, 2699
HapII	CCGG	4 cut(s)	311, 321, 2497, 2701
HgaI	GACGC	3 cut(s)	1160, 2990, 3119
HhaI	GCGC	1 cut(s)	1974
Hin1I	GRCGYC	1 cut(s)	1171
Hin6I	GCGC	1 cut(s)	1972
HinP1I	GCGC	1 cut(s)	1972
HpaII	CCGG	4 cut(s)	311, 321, 2497, 2701
Hpy166II	GTNNAC	7 cut(s)	365, 512, 1355, 1371, 2457, 2612, 3005
Hpy8I	GTNNAC	7 cut(s)	365, 512, 1355, 1371, 2457, 2612, 3005
HpyAV	CCTTC	9 cut(s)	83, 401, 526, 873, 1008, 1534, 1710, 2233, 2410
HpyCH4IV	ACGT	3 cut(s)	2068, 3007, 3208
HpySE526I	ACGT	3 cut(s)	2068, 3007, 3208
Hsp92I	GRCGYC	1 cut(s)	1171
HspAI	GCGC	1 cut(s)	1972
Ksp22I	TGATCA	1 cut(s)	193
LguI	GCTCTTC	1 cut(s)	1201
LmnI	GCTCC	5 cut(s)	1157, 1416, 1733, 3018, 3052
Lsp1109I	GCAGC	8 cut(s)	88, 243, 2219, 2681, 2932, 2935, 3168, 3307
LweI	GCATC	9 cut(s)	243, 463, 571, 772, 2143, 2298, 2885, 2919, 3187
MabI	ACCWGGT	1 cut(s)	770
MaeI	CTAG	6 cut(s)	1358, 2076, 2109, 2220, 2469, 3222
MaeII	ACGT	3 cut(s)	2068, 3007, 3208
MbiI	CCGCTC	1 cut(s)	24
MfeI	CAATTG	3 cut(s)	1292, 2615, 3382
MflI	RGATCY	1 cut(s)	2306
MhlI	GDGCHC	6 cut(s)	245, 1196, 2268, 2459, 2755, 2821
MlyI	GAGTC	3 cut(s)	232, 605, 1641
MmeI	TCCRAC	7 cut(s)	846, 865, 1306, 1720, 2207, 2384, 3070
Mph1103I	ATGCAT	1 cut(s)	2878
MroXI	GAANNNNTTC	4 cut(s)	176, 1482, 1719, 3099
MslI	CAYNNNNRTG	8 cut(s)	459, 1042, 1599, 1776, 2162, 2538, 2881, 3240
MspA1I	CMGCKG	1 cut(s)	3016
MspI	CCGG	4 cut(s)	311, 321, 2497, 2701
MspR9I	CCNGG	6 cut(s)	758, 772, 1140, 1644, 2498, 2701
MunI	CAATTG	3 cut(s)	1292, 2615, 3382
Mva1269I	GAATGC	5 cut(s)	891, 936, 970, 2098, 2221
MvaI	CCWGG	4 cut(s)	758, 772, 1140, 1644
MvnI	CGCG	1 cut(s)	3126
NciI	CCSGG	2 cut(s)	2498, 2701
NcoI	CCATGG	3 cut(s)	1838, 2448, 2533
NlaIV	GGNNCC	6 cut(s)	64, 1240, 2308, 2332, 3054, 3401
NmuCI	GTSAC	7 cut(s)	628, 1067, 1442, 1613, 1677, 1916, 2790
NsiI	ATGCAT	1 cut(s)	2878
NspI	RCATGY	4 cut(s)	297, 846, 1740, 1887
NspV	TTCGAA	1 cut(s)	438
PaeI	GCATGC	1 cut(s)	1887
PaeR7I	CTCGAG	1 cut(s)	238
PagI	TCATGA	1 cut(s)	1099
PciI	ACATGT	2 cut(s)	293, 842
PciSI	GCTCTTC	1 cut(s)	1201
PctI	GAATGC	5 cut(s)	891, 936, 970, 2098, 2221
PdmI	GAANNNNTTC	4 cut(s)	176, 1482, 1719, 3099
PflFI	GACNNNGTC	1 cut(s)	772
PfoI	TCCNGGA	1 cut(s)	2496
PinAI	ACCGGT	2 cut(s)	310, 320
PkrI	GCNGC	9 cut(s)	62, 78, 233, 2209, 2671, 2947, 2950, 3158, 3322
PleI	GAGTC	3 cut(s)	231, 604, 1641
PpsI	GAGTC	3 cut(s)	231, 604, 1641
Ppu21I	YACGTR	1 cut(s)	2069
PpuMI	RGGWCCY	1 cut(s)	1238
PscI	ACATGT	2 cut(s)	293, 842
PshBI	ATTAAT	2 cut(s)	152, 2766
PsiI	TTATAA	2 cut(s)	3269, 3348
Psp124BI	GAGCTC	2 cut(s)	1196, 2821
Psp5II	RGGWCCY	1 cut(s)	1238
Psp6I	CCWGG	4 cut(s)	756, 770, 1138, 1642
PspEI	GGTNACC	1 cut(s)	1613
PspFI	CCCAGC	1 cut(s)	2772
PspGI	CCWGG	4 cut(s)	756, 770, 1138, 1642
PspN4I	GGNNCC	6 cut(s)	64, 1240, 2308, 2332, 3054, 3401
PspPI	GGNCC	8 cut(s)	62, 422, 557, 602, 774, 1238, 2698, 2703
PspPPI	RGGWCCY	1 cut(s)	1238
PstI	CTGCAG	1 cut(s)	1936
PsuI	RGATCY	1 cut(s)	2306
PsyI	GACNNNGTC	1 cut(s)	772
RsaI	GTAC	2 cut(s)	298, 1262
RsaNI	GTAC	2 cut(s)	297, 1261
RseI	CAYNNNNRTG	8 cut(s)	459, 1042, 1599, 1776, 2162, 2538, 2881, 3240
SacI	GAGCTC	2 cut(s)	1196, 2821
SapI	GCTCTTC	1 cut(s)	1201
SatI	GCNGC	9 cut(s)	61, 77, 232, 2208, 2670, 2946, 2949, 3157, 3321
Sau96I	GGNCC	8 cut(s)	62, 422, 557, 602, 774, 1238, 2698, 2703
SchI	GAGTC	3 cut(s)	232, 605, 1641
ScrFI	CCNGG	6 cut(s)	758, 772, 1140, 1644, 2498, 2701
SduI	GDGCHC	6 cut(s)	245, 1196, 2268, 2459, 2755, 2821
SexAI	ACCWGGT	1 cut(s)	770
SfaNI	GCATC	9 cut(s)	243, 463, 571, 772, 2143, 2298, 2885, 2919, 3187
SfcI	CTRYAG	2 cut(s)	1746, 1932
Sfr274I	CTCGAG	1 cut(s)	238
SfuI	TTCGAA	1 cut(s)	438
SinI	GGWCC	4 cut(s)	557, 774, 1238, 2703
SlaI	CTCGAG	1 cut(s)	238
SmiMI	CAYNNNNRTG	8 cut(s)	459, 1042, 1599, 1776, 2162, 2538, 2881, 3240
SmlI	CTYRAG	2 cut(s)	238, 2660
SmoI	CTYRAG	2 cut(s)	238, 2660
SpeI	ACTAGT	1 cut(s)	1357
SphI	GCATGC	1 cut(s)	1887
SsiI	CCGC	6 cut(s)	24, 60, 1475, 1759, 1984, 3016
SspI	AATATT	2 cut(s)	995, 1399
SspMI	CTAG	6 cut(s)	1358, 2076, 2109, 2220, 2469, 3222
SstI	GAGCTC	2 cut(s)	1196, 2821
StyD4I	CCNGG	6 cut(s)	756, 770, 1138, 1642, 2496, 2699
StyI	CCWWGG	6 cut(s)	777, 1838, 2448, 2533, 3048, 3261
TaiI	ACGT	3 cut(s)	2071, 3010, 3211
TaqI	TCGA	5 cut(s)	239, 438, 457, 1953, 3060
TaqII	GACCGA	1 cut(s)	1331
TatI	WGTACW	2 cut(s)	296, 1260
TauI	GCSGC	1 cut(s)	63
TscAI	CASTG	8 cut(s)	528, 990, 1936, 1953, 2052, 2377, 2512, 2888
TseFI	GTSAC	7 cut(s)	628, 1067, 1442, 1613, 1677, 1916, 2790
TseI	GCWGC	8 cut(s)	76, 231, 2207, 2669, 2945, 2948, 3156, 3320
Tsp45I	GTSAC	7 cut(s)	628, 1067, 1442, 1613, 1677, 1916, 2790
TspGWI	ACGGA	2 cut(s)	675, 1856
TspRI	CASTG	8 cut(s)	528, 990, 1936, 1953, 2052, 2377, 2512, 2888
Tth111I	GACNNNGTC	1 cut(s)	772
VneI	GTGCAC	1 cut(s)	2455
VpaK11BI	GGWCC	4 cut(s)	557, 774, 1238, 2703
VspI	ATTAAT	2 cut(s)	152, 2766
XapI	RAATTY	6 cut(s)	259, 445, 575, 1104, 1381, 1691
XceI	RCATGY	4 cut(s)	297, 846, 1740, 1887
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2377
XhoI	CTCGAG	1 cut(s)	238
XmiI	GTMKAC	2 cut(s)	364, 3004
XmnI	GAANNNNTTC	4 cut(s)	176, 1482, 1719, 3099
XspI	CTAG	6 cut(s)	1358, 2076, 2109, 2220, 2469, 3222
Zsp2I	ATGCAT	1 cut(s)	2878

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.