Rroxscaffold_3G00249030

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0017319
Physical Location & Seq
Forward (+)
42562436 .. 42567253
4818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00249030.1

Sequence Viewer

Length: 1500 bp
ATGAAAGACTCTTCAGATTCAGGAGCTCAAGATCTTCACCCTCCTCCTTCTCAAGGAAATGAGGACTCTGAATTTCTCCCTGCTGCGGAAGATCTTCACCCTCCTCCTTTTCACCCTCCTCGTTCTCAAGGAAATGAGGACTCTGAGGGCTCTGAATATCTCAATGAGTTGGCCGACTTTCTGGAGCTGGAGCTGGGCGTTGTACCTGAGATGAAAGACTCTTCAGATTCAGATTCTGGAGCGCAAGATCTTGACACTCCTCCTTCTCAAGGAAATGAGGACTCTGCATTTGTCAATGATTTGGCCACCTTTCATACCCAAACCCAAGTTACTGCGCCGGTCACCTTTCAAACCCTCAGAGCTGCCGTTTTGAATGAGTTGGCCGCCTTTCAAACCCAACTAACTGCTGCGGTCAGAGCTGCCGTTTTGGACCTCTTGAGAGTAGAGAGGAAGGTTTTTAAGGACCAAGTGAGTGCTATCTTGAGCCAAGAGGTGAACCAGCAGATATTGGCTTGGAGAGAAGAGTACAGGATCAAGGAAGAATCTGAATTGGTCGCCCGTCTCTTCCAATTGATCGGTCAGATCGGTCGGAGGAAGAAACCGGAGGAGGACGAGGGAGATGAAGTGGAAGAGGAAGAGGAGGATAGTCAACCTCGAGAAGAAGAACACGATCTGAAGCTGGATGTCATTGCTGCGGTCAAGGCTGCCGCTGTGGACCTCTTGGCTAAAGAGATGAAAGTTTTGAAGGACCAAGTGGGTGCTGTCTTGAGGCAAGAGGCGAACCAGCATTTCAATGCTTTGAGAGAACAGTTTAAGGCGTCTGTGTGTAAAGTGGAGGAAGAACCGAAGCCTTCCTTAATGGACCATTTCTGTGTCGATCCAAGGCCTTCTGATGGCGACTTTTGTGGGACACCTGTTGTGGATTATCATGTTGTGGCAATCAGTAAGATGCTCCAGTCATGCTCTGTACCACTGGGAGAGAGCATTGGCAACCTGCTGCATAGAGGGATCAAACAGTGGGCTCCCAAGCTTCGCACCAACACGGCTCTGGTAGAATTTTCCTCACCCGAAACAGCTGGTGATATGCGTCTCTTGCACTTTGAAGCAGCAATAATGGGGGAATCTATAGCCCGAATGCTCCAATTTTCGGATGTGGAAGTTCTGCGTAGCACCCATGTCATTGCTTGTGGGTCACAGGGTTGGGAACCCATCTGCTCCTCTGTGTGGAAGCCGATGCAAGATGATACCCTTGAAGACTTGCGCAATATGTATGCGCTCGCAGGAGTGCAATGGCAAGATGAGGACGGGACCTTCTATTCATATAAGGAGGAGGTCCAAAAGAAATTGCAAGAGAAGTTGGATGATTGGCGCTCTATCCTGCCATCTGGAGTTGTCCTAACTGAGCTCATTTCTCTTCGGTATCGGATAGACATTCAGAAAGCTGAATGGCTTATCTATGTTACCCCCAGAACACAAAGATCATTTCAATACGGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000049 GO:0000122 GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004814 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006355 GO:0006357 GO:0006399 GO:0006412 GO:0006418 GO:0006420 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0007568 GO:0008144 GO:0008150 GO:0008152 GO:0008340 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010259 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0017076 GO:0017101 GO:0019219 GO:0019222 GO:0019538 GO:0019752 GO:0030554 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031406 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0034618 GO:0034641 GO:0034645 GO:0034660 GO:0035639 GO:0036094 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048518 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097367 GO:0140098 GO:0140101 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Arg_tRNA_synt_N DALR_1 tRNA-synt_1d

Protein Analysis

499

Amino Acids

56.09

Weight (kDa)

4.6

Isoelectric Point (pI)

50.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1d PF00750 332 - 460 4.5e-09 tRNA synthetases class I (R), catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1262
Acc36I ACCTGC 1 cut(s) 1002
AciI CCGC 5 cut(s) 86, 384, 410, 695, 708
AclWI GGATC 3 cut(s) 539, 872, 1016
AcoI YGGCCR 3 cut(s) 171, 303, 381
AcsI RAATTY 2 cut(s) 71, 1055
AcuI CTGAAG 2 cut(s) 207, 695
AcyI GRCGYC 1 cut(s) 818
AfaI GTAC 3 cut(s) 204, 527, 969
AfiI CCNNNNNNNGG 6 cut(s) 53, 85, 269, 893, 1147, 1224
AgsI TTSAA 8 cut(s) 350, 373, 392, 745, 793, 1103, 1253, 1487
Alw21I GWGCWC 2 cut(s) 28, 1407
Alw26I GTCTC 2 cut(s) 566, 1094
AlwI GGATC 3 cut(s) 539, 872, 1016
AlwNI CAGNNNCTG 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 654
AoxI GGCC 4 cut(s) 171, 303, 381, 884
ApeKI GCWGC 8 cut(s) 83, 362, 407, 419, 692, 704, 997, 1106
ApoI RAATTY 2 cut(s) 71, 1055
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
Asp700I GAANNNNTTC 1 cut(s) 93
AspLEI GCGC 5 cut(s) 244, 337, 1263, 1276, 1371
AspS9I GGNCC 7 cut(s) 430, 463, 715, 748, 862, 1308, 1333
AsuHPI GGTGA 7 cut(s) 29, 89, 104, 334, 505, 1056, 1091
AvaI CYCGRG 1 cut(s) 654
AvaII GGWCC 7 cut(s) 430, 463, 715, 748, 862, 1308, 1333
BalI TGGCCA 1 cut(s) 305
BanII GRGCYC 4 cut(s) 28, 152, 1024, 1407
BbsI GAAGAC 1 cut(s) 1260
Bbv12I GWGCWC 2 cut(s) 28, 1407
BbvI GCAGC 8 cut(s) 70, 349, 394, 406, 679, 691, 984, 1118
BccI CCATC 3 cut(s) 887, 1217, 1390
BceAI ACGGC 3 cut(s) 350, 407, 1059
BcoDI GTCTC 2 cut(s) 566, 1094
BfmI CTRYAG 1 cut(s) 1125
BfoI RGCGCY 1 cut(s) 1372
BfuAI ACCTGC 1 cut(s) 1002
BglII AGATCT 3 cut(s) 31, 91, 247
Bme18I GGWCC 7 cut(s) 430, 463, 715, 748, 862, 1308, 1333
BmeT110I CYCGRG 1 cut(s) 654
BmgT120I GGNCC 7 cut(s) 430, 463, 715, 748, 862, 1308, 1333
BmiI GGNNCC 3 cut(s) 1023, 1206, 1309
BmrI ACTGGG 1 cut(s) 983
BmsI GCATC 2 cut(s) 939, 1224
BmuI ACTGGG 1 cut(s) 983
BpiI GAAGAC 1 cut(s) 1260
BpmI CTGGAG 5 cut(s) 203, 209, 258, 938, 1407
BpuEI CTTGAG 7 cut(s) 12, 36, 111, 252, 457, 502, 787
BsaHI GRCGYC 1 cut(s) 818
BsaJI CCNNGG 1 cut(s) 881
BsaWI WCCGGW 1 cut(s) 601
BsaXI ACNNNNNCTCC 2 cut(s) 609, 639
Bsc4I CCNNNNNNNGG 6 cut(s) 53, 85, 269, 893, 1147, 1224
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 2 cut(s) 955, 978
Bse3DI GCAATG 3 cut(s) 687, 1179, 1295
BseDI CCNNGG 1 cut(s) 881
BseGI GGATG 3 cut(s) 688, 1156, 1366
BseLI CCNNNNNNNGG 6 cut(s) 53, 85, 269, 893, 1147, 1224
BseMI GCAATG 3 cut(s) 687, 1179, 1295
BseMII CTCAG 4 cut(s) 135, 198, 370, 1392
BseNI ACTGG 2 cut(s) 955, 978
BseRI GAGGAG 8 cut(s) 33, 93, 108, 249, 620, 653, 1207, 1343
BseXI GCAGC 8 cut(s) 70, 349, 394, 406, 679, 691, 984, 1118
BseYI CCCAGC 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 589
BshFI GGCC 4 cut(s) 173, 305, 383, 886
BsiEI CGRYCG 1 cut(s) 589
BsiHKAI GWGCWC 2 cut(s) 28, 1407
BsiHKCI CYCGRG 1 cut(s) 654
BsiSI CCGG 2 cut(s) 338, 602
BslFI GGGAC 2 cut(s) 922, 1321
BslI CCNNNNNNNGG 6 cut(s) 53, 85, 269, 893, 1147, 1224
BsmAI GTCTC 2 cut(s) 566, 1094
BsmBI CGTCTC 2 cut(s) 566, 1094
BsmFI GGGAC 2 cut(s) 922, 1321
BsmI GAATGC 1 cut(s) 1140
BsnI GGCC 4 cut(s) 173, 305, 383, 886
BsoBI CYCGRG 1 cut(s) 654
Bsp1286I GDGCHC 4 cut(s) 28, 152, 1024, 1407
BspACI CCGC 5 cut(s) 86, 384, 410, 695, 708
BspANI GGCC 4 cut(s) 173, 305, 383, 886
BspCNI CTCAG 4 cut(s) 136, 199, 369, 1393
BspLI GGNNCC 3 cut(s) 1023, 1206, 1309
BspMI ACCTGC 1 cut(s) 1002
BspPI GGATC 3 cut(s) 539, 872, 1016
BsrDI GCAATG 3 cut(s) 687, 1179, 1295
BsrFI RCCGGY 1 cut(s) 337
BsrI ACTGG 2 cut(s) 955, 978
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 1 cut(s) 881
BssNI GRCGYC 1 cut(s) 818
BssT1I CCWWGG 1 cut(s) 881
Bst4CI ACNGT 3 cut(s) 810, 1017, 1493
Bst6I CTCTTC 7 cut(s) 16, 226, 516, 569, 624, 630, 1419
BstACI GRCGYC 1 cut(s) 818
BstC8I GCNNGC 1 cut(s) 1278
BstDEI CTNAG 4 cut(s) 144, 207, 356, 1401
BstEII GGTNACC 1 cut(s) 340
BstENI CCTNNNNNAGG 2 cut(s) 51, 267
BstF5I GGATG 3 cut(s) 688, 1156, 1366
BstH2I RGCGCY 1 cut(s) 1372
BstHHI GCGC 5 cut(s) 244, 337, 1263, 1276, 1371
BstMAI GTCTC 2 cut(s) 566, 1094
BstMCI CGRYCG 1 cut(s) 589
BstMWI GCNNNNNNNGC 3 cut(s) 416, 701, 1093
BstPI GGTNACC 1 cut(s) 340
BstSFI CTRYAG 1 cut(s) 1125
BstV1I GCAGC 8 cut(s) 70, 349, 394, 406, 679, 691, 984, 1118
BstV2I GAAGAC 1 cut(s) 1260
BstX2I RGATCY 3 cut(s) 31, 91, 247
BstYI RGATCY 3 cut(s) 31, 91, 247
BsuRI GGCC 4 cut(s) 173, 305, 383, 886
BtsCI GGATG 3 cut(s) 688, 1156, 1366
BtsIMutI CAGTG 2 cut(s) 971, 1022
BveI ACCTGC 1 cut(s) 1002
Cac8I GCNNGC 1 cut(s) 1278
CaiI CAGNNNCTG 1 cut(s) 236
CfoI GCGC 5 cut(s) 244, 337, 1263, 1276, 1371
Cfr10I RCCGGY 1 cut(s) 337
Cfr13I GGNCC 7 cut(s) 430, 463, 715, 748, 862, 1308, 1333
CseI GACGC 2 cut(s) 807, 1076
Csp6I GTAC 3 cut(s) 203, 526, 968
CviAII CATG 3 cut(s) 929, 960, 1175
CviQI GTAC 3 cut(s) 203, 526, 968
DdeI CTNAG 4 cut(s) 144, 207, 356, 1401
EaeI YGGCCR 3 cut(s) 171, 303, 381
Eam1104I CTCTTC 7 cut(s) 16, 226, 516, 569, 624, 630, 1419
EarI CTCTTC 7 cut(s) 16, 226, 516, 569, 624, 630, 1419
Ecl136II GAGCTC 2 cut(s) 26, 1405
Eco130I CCWWGG 1 cut(s) 881
Eco147I AGGCCT 1 cut(s) 886
Eco24I GRGCYC 4 cut(s) 28, 152, 1024, 1407
Eco47I GGWCC 7 cut(s) 430, 463, 715, 748, 862, 1308, 1333
Eco53kI GAGCTC 2 cut(s) 26, 1405
Eco57I CTGAAG 2 cut(s) 207, 695
Eco88I CYCGRG 1 cut(s) 654
Eco91I GGTNACC 1 cut(s) 340
EcoICRI GAGCTC 2 cut(s) 26, 1405
EcoNI CCTNNNNNAGG 2 cut(s) 51, 267
EcoO109I RGGNCCY 1 cut(s) 1308
EcoO65I GGTNACC 1 cut(s) 340
EcoT14I CCWWGG 1 cut(s) 881
EcoT38I GRGCYC 4 cut(s) 28, 152, 1024, 1407
ErhI CCWWGG 1 cut(s) 881
Esp3I CGTCTC 2 cut(s) 566, 1094
FaeI CATG 3 cut(s) 932, 963, 1178
FalI AAGNNNNNCTT 2 cut(s) 839, 871
FaqI GGGAC 2 cut(s) 922, 1321
FatI CATG 3 cut(s) 928, 959, 1174
FokI GGATG 3 cut(s) 695, 1163, 1373
FriOI GRGCYC 4 cut(s) 28, 152, 1024, 1407
FspI TGCGCA 1 cut(s) 1262
GlaI GCGC 5 cut(s) 243, 336, 1262, 1275, 1370
GsaI CCCAGC 1 cut(s) 197
GsuI CTGGAG 5 cut(s) 203, 209, 258, 938, 1407
HaeII RGCGCY 1 cut(s) 1372
HaeIII GGCC 4 cut(s) 173, 305, 383, 886
HapII CCGG 2 cut(s) 338, 602
HgaI GACGC 2 cut(s) 807, 1076
HhaI GCGC 5 cut(s) 244, 337, 1263, 1276, 1371
Hin1I GRCGYC 1 cut(s) 818
Hin1II CATG 3 cut(s) 932, 963, 1178
Hin6I GCGC 5 cut(s) 242, 335, 1261, 1274, 1369
HinP1I GCGC 5 cut(s) 242, 335, 1261, 1274, 1369
HincII GTYRAC 1 cut(s) 650
HindII GTYRAC 1 cut(s) 650
HindIII AAGCTT 1 cut(s) 1028
HpaII CCGG 2 cut(s) 338, 602
HphI GGTGA 7 cut(s) 29, 89, 104, 334, 505, 1056, 1091
Hpy166II GTNNAC 3 cut(s) 496, 650, 715
Hpy8I GTNNAC 3 cut(s) 496, 650, 715
HpyAV CCTTC 7 cut(s) 57, 273, 445, 739, 861, 897, 1321
HpyCH4III ACNGT 3 cut(s) 810, 1017, 1493
HpyCH4V TGCA 6 cut(s) 287, 1000, 1096, 1237, 1288, 1348
HpyF10VI GCNNNNNNNGC 3 cut(s) 416, 701, 1093
HpyF3I CTNAG 4 cut(s) 144, 207, 356, 1401
Hsp92I GRCGYC 1 cut(s) 818
Hsp92II CATG 3 cut(s) 932, 963, 1178
HspAI GCGC 5 cut(s) 242, 335, 1261, 1274, 1369
LmnI GCTCC 8 cut(s) 23, 184, 190, 239, 957, 1027, 1143, 1220
Lsp1109I GCAGC 8 cut(s) 70, 349, 394, 406, 679, 691, 984, 1118
LweI GCATC 2 cut(s) 939, 1224
MaeIII GTNAC 4 cut(s) 328, 340, 1191, 1459
MfeI CAATTG 1 cut(s) 569
MflI RGATCY 3 cut(s) 31, 91, 247
MhlI GDGCHC 4 cut(s) 28, 152, 1024, 1407
MlsI TGGCCA 1 cut(s) 305
MluCI AATT 6 cut(s) 71, 548, 569, 1055, 1142, 1343
MluNI TGGCCA 1 cut(s) 305
MlyI GAGTC 5 cut(s) 2, 59, 134, 212, 275
MmeI TCCRAC 2 cut(s) 569, 1338
Mox20I TGGCCA 1 cut(s) 305
MroXI GAANNNNTTC 1 cut(s) 93
MscI TGGCCA 1 cut(s) 305
MseI TTAA 4 cut(s) 459, 813, 857, 1498
MslI CAYNNNNRTG 2 cut(s) 792, 870
Msp20I TGGCCA 1 cut(s) 305
MspA1I CMGCKG 2 cut(s) 710, 1076
MspI CCGG 2 cut(s) 338, 602
MunI CAATTG 1 cut(s) 569
Mva1269I GAATGC 1 cut(s) 1140
MwoI GCNNNNNNNGC 3 cut(s) 416, 701, 1093
NlaIII CATG 3 cut(s) 932, 963, 1178
NlaIV GGNNCC 3 cut(s) 1023, 1206, 1309
NmuCI GTSAC 2 cut(s) 340, 1191
NsbI TGCGCA 1 cut(s) 1262
PaeR7I CTCGAG 1 cut(s) 654
PceI AGGCCT 1 cut(s) 886
PctI GAATGC 1 cut(s) 1140
PdmI GAANNNNTTC 1 cut(s) 93
PfeI GAWTC 5 cut(s) 17, 227, 233, 542, 1121
PleI GAGTC 5 cut(s) 2, 59, 134, 212, 275
PpsI GAGTC 5 cut(s) 2, 59, 134, 212, 275
PpuMI RGGWCCY 1 cut(s) 1308
Psp124BI GAGCTC 2 cut(s) 28, 1407
Psp5II RGGWCCY 1 cut(s) 1308
PspEI GGTNACC 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 193
PspN4I GGNNCC 3 cut(s) 1023, 1206, 1309
PspPI GGNCC 7 cut(s) 430, 463, 715, 748, 862, 1308, 1333
PspPPI RGGWCCY 1 cut(s) 1308
PstNI CAGNNNCTG 1 cut(s) 236
PsuI RGATCY 3 cut(s) 31, 91, 247
PvuII CAGCTG 1 cut(s) 1076
RsaI GTAC 3 cut(s) 204, 527, 969
RsaNI GTAC 3 cut(s) 203, 526, 968
RseI CAYNNNNRTG 2 cut(s) 792, 870
SacI GAGCTC 2 cut(s) 28, 1407
SaqAI TTAA 4 cut(s) 459, 813, 857, 1498
Sau96I GGNCC 7 cut(s) 430, 463, 715, 748, 862, 1308, 1333
SchI GAGTC 5 cut(s) 2, 59, 134, 212, 275
SduI GDGCHC 4 cut(s) 28, 152, 1024, 1407
SfaNI GCATC 2 cut(s) 939, 1224
SfcI CTRYAG 1 cut(s) 1125
Sfr274I CTCGAG 1 cut(s) 654
SinI GGWCC 7 cut(s) 430, 463, 715, 748, 862, 1308, 1333
SlaI CTCGAG 1 cut(s) 654
SmiMI CAYNNNNRTG 2 cut(s) 792, 870
SmlI CTYRAG 8 cut(s) 27, 51, 126, 267, 436, 481, 654, 766
SmoI CTYRAG 8 cut(s) 27, 51, 126, 267, 436, 481, 654, 766
Sse9I AATT 6 cut(s) 71, 548, 569, 1055, 1142, 1343
SseBI AGGCCT 1 cut(s) 886
SsiI CCGC 5 cut(s) 86, 384, 410, 695, 708
SstI GAGCTC 2 cut(s) 28, 1407
StuI AGGCCT 1 cut(s) 886
StyI CCWWGG 1 cut(s) 881
TaaI ACNGT 3 cut(s) 810, 1017, 1493
TaqI TCGA 2 cut(s) 655, 876
TaqII GACCGA 2 cut(s) 566, 575
TasI AATT 6 cut(s) 71, 548, 569, 1055, 1142, 1343
TatI WGTACW 1 cut(s) 525
TauI GCSGC 2 cut(s) 386, 710
TfiI GAWTC 5 cut(s) 17, 227, 233, 542, 1121
Tru1I TTAA 4 cut(s) 459, 813, 857, 1498
Tru9I TTAA 4 cut(s) 459, 813, 857, 1498
TscAI CASTG 2 cut(s) 978, 1022
TseFI GTSAC 2 cut(s) 340, 1191
TseI GCWGC 8 cut(s) 83, 362, 407, 419, 692, 704, 997, 1106
Tsp45I GTSAC 2 cut(s) 340, 1191
TspDTI ATGAA 6 cut(s) 17, 227, 302, 636, 749, 1308
TspRI CASTG 2 cut(s) 978, 1022
VpaK11BI GGWCC 7 cut(s) 430, 463, 715, 748, 862, 1308, 1333
XagI CCTNNNNNAGG 2 cut(s) 51, 267
XapI RAATTY 2 cut(s) 71, 1055
XcmI CCANNNNNNNNNTGG 1 cut(s) 1045
XhoI CTCGAG 1 cut(s) 654
XmnI GAANNNNTTC 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.