Rroxscaffold_3G00250830

Protein GLUTAMINE DUMPER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008129
Physical Location & Seq
Forward (+)
44676104 .. 44683785
7682 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00250830.1

Sequence Viewer

Length: 957 bp
ATGGGTGACGAACGTACTACCCCAGTTACAAGAGTGGATTTGGATGCCCTTACTGCTCAAATGACCGCTGCCGTGAATGCTCAAATGGCAACTATGAACGCTCAAATGGCAGGAACAACACCACCAACTGTAACGGAGGCGGGGAAGGACAACATGTTAGGGCTCCACATGGTGGTGGAGCGGAACTCAAGGGTGAGTTCTTTCATAGTGGAGGAGAATGATGAGTGGCGTGGAATCAAGAAGAGTGATCGGAAAATAGTGCTTTTTGCAAGTGGGGAATGGAAAGGTTTGGAAAGTGAGATGAAGGAATACAAAGAAGGAAAAGTCTCTCATATAATGACCCCTGCTCTGGACTTCGTGTCATGGCAAATTTGTTCGGTGGGTTTGTTGCGGTTGATTTTTGAAGCCCCATTTTCAAATCCTGGTTCGTCTGTTGGCACAGCTCGGGTCCAACTCATAGTCAGGCTCACATTCCTCACCTGCTTCCCCTCTAAACAAAACCTAGATCCCCACTATCTTCAGAAAATGAGAGCCACCGTAATGGCTACTATTTCACCAACAAAACTACCTTCAACAGAAGCACCACCAGACATGGCTCCACCGGTTTCACCTTGGCACTTTCCGATCCCATACCTCTTAGGTGGTCTAGTGACGATGGCTATTCTTGTTGTTTGTGCTCTCTTGATCTTGGCTTGCTCATTTTGGAAGCGACACAGAGGAGGCAGCAGTGCCAACAGAGATTTGGAACTGGGTGGGAATTCTGTGAAGGTGTTTAAAGACAAAGTTTTGGTTATAATGGCCGGAAATGAGAACCCTACTTACTTGGCCACCCCAATTCCGATTTGCCATACTAAAAGTACTAGTTTTGAAGAGGAATTAGAGAAGATAGAAGGAGATGATAAGGAAGAGAACTGTAAGAAATCGAAAGAGGCAGCATCATCAACATCAGCGAGCTAG

Protein Analysis

318

Amino Acids

34.85

Weight (kDa)

6.34

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 86 - 153 1.1e-12 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 794
AarI CACCTGC 1 cut(s) 488
Acc36I ACCTGC 1 cut(s) 488
AccB7I CCANNNNNTGG 1 cut(s) 172
AccBSI CCGCTC 1 cut(s) 181
AciI CCGC 4 cut(s) 66, 140, 181, 391
AclWI GGATC 2 cut(s) 500, 619
AcoI YGGCCR 2 cut(s) 798, 825
AcsI RAATTY 2 cut(s) 369, 757
AcuI CTGAAG 1 cut(s) 503
AdeI CACNNNGTG 1 cut(s) 172
AfaI GTAC 2 cut(s) 16, 859
AfiI CCNNNNNNNGG 2 cut(s) 172, 349
AflIII ACRYGT 1 cut(s) 153
AgeI ACCGGT 1 cut(s) 601
AgsI TTSAA 4 cut(s) 404, 417, 573, 869
AhdI GACNNNNNGTC 1 cut(s) 358
AhlI ACTAGT 1 cut(s) 860
AjnI CCWGG 1 cut(s) 421
AluBI AGCT 2 cut(s) 443, 954
AluI AGCT 2 cut(s) 443, 954
Alw21I GWGCWC 1 cut(s) 679
Alw26I GTCTC 1 cut(s) 331
AlwI GGATC 2 cut(s) 500, 619
Ama87I CYCGRG 1 cut(s) 444
AoxI GGCC 2 cut(s) 798, 825
ApeKI GCWGC 3 cut(s) 68, 723, 932
ApoI RAATTY 2 cut(s) 369, 757
AsiGI ACCGGT 1 cut(s) 601
AspS9I GGNCC 1 cut(s) 448
AsuHPI GGTGA 5 cut(s) 17, 205, 469, 546, 600
AvaI CYCGRG 1 cut(s) 444
AvaII GGWCC 1 cut(s) 448
BalI TGGCCA 1 cut(s) 827
BanII GRGCYC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 679
BbvI GCAGC 3 cut(s) 55, 735, 944
BccI CCATC 1 cut(s) 649
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 1 cut(s) 423
BcoDI GTCTC 1 cut(s) 331
BcuI ACTAGT 1 cut(s) 860
BfaI CTAG 4 cut(s) 503, 647, 861, 955
BfuAI ACCTGC 1 cut(s) 488
BisI GCNGC 3 cut(s) 69, 724, 933
BlsI GCNGC 3 cut(s) 70, 725, 934
BmcAI AGTACT 1 cut(s) 859
Bme1390I CCNGG 1 cut(s) 423
Bme18I GGWCC 1 cut(s) 448
BmeRI GACNNNNNGTC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 444
BmgT120I GGNCC 1 cut(s) 448
BmiI GGNNCC 3 cut(s) 164, 449, 597
BmrFI CCNGG 1 cut(s) 423
BmrI ACTGGG 2 cut(s) 17, 758
BmsI GCATC 2 cut(s) 34, 944
BmuI ACTGGG 2 cut(s) 17, 758
BplI GAGNNNNNCTC 2 cut(s) 170, 202
BpuEI CTTGAG 1 cut(s) 172
BsaJI CCNNGG 1 cut(s) 611
BsaWI WCCGGW 1 cut(s) 601
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 349
Bse118I RCCGGY 1 cut(s) 601
Bse1I ACTGG 2 cut(s) 23, 753
BseBI CCWGG 1 cut(s) 423
BseDI CCNNGG 1 cut(s) 611
BseGI GGATG 1 cut(s) 49
BseLI CCNNNNNNNGG 2 cut(s) 172, 349
BseNI ACTGG 2 cut(s) 23, 753
BseRI GAGGAG 2 cut(s) 227, 732
BseXI GCAGC 3 cut(s) 55, 735, 944
BshFI GGCC 2 cut(s) 800, 827
BshTI ACCGGT 1 cut(s) 601
BsiHKAI GWGCWC 1 cut(s) 679
BsiHKCI CYCGRG 1 cut(s) 444
BsiSI CCGG 2 cut(s) 602, 801
BslI CCNNNNNNNGG 2 cut(s) 172, 349
BsmAI GTCTC 1 cut(s) 331
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 2 cut(s) 800, 827
BsoBI CYCGRG 1 cut(s) 444
Bsp1286I GDGCHC 2 cut(s) 165, 679
Bsp143I GATC 4 cut(s) 247, 505, 624, 684
BspACI CCGC 4 cut(s) 66, 140, 181, 391
BspANI GGCC 2 cut(s) 800, 827
BspLI GGNNCC 3 cut(s) 164, 449, 597
BspMI ACCTGC 1 cut(s) 488
BspPI GGATC 2 cut(s) 500, 619
BsrBI CCGCTC 1 cut(s) 181
BsrFI RCCGGY 1 cut(s) 601
BsrI ACTGG 2 cut(s) 23, 753
BssAI RCCGGY 1 cut(s) 601
BssECI CCNNGG 1 cut(s) 611
BssMI GATC 4 cut(s) 247, 505, 624, 684
BssT1I CCWWGG 1 cut(s) 611
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 3 cut(s) 130, 538, 914
Bst6I CTCTTC 3 cut(s) 236, 864, 900
BstC8I GCNNGC 2 cut(s) 694, 952
BstDEI CTNAG 1 cut(s) 637
BstF5I GGATG 1 cut(s) 49
BstKTI GATC 4 cut(s) 250, 508, 627, 687
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 4 cut(s) 247, 505, 624, 684
BstMWI GCNNNNNNNGC 4 cut(s) 53, 77, 86, 107
BstNI CCWGG 1 cut(s) 423
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 1 cut(s) 421
BstV1I GCAGC 3 cut(s) 55, 735, 944
BstX2I RGATCY 1 cut(s) 505
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 1 cut(s) 505
BsuRI GGCC 2 cut(s) 800, 827
BtsCI GGATG 1 cut(s) 49
BtsI GCAGTG 1 cut(s) 733
BtsIMutI CAGTG 1 cut(s) 733
BveI ACCTGC 1 cut(s) 488
Cac8I GCNNGC 2 cut(s) 694, 952
Cfr10I RCCGGY 1 cut(s) 601
Cfr13I GGNCC 1 cut(s) 448
Csp6I GTAC 2 cut(s) 15, 858
CspAI ACCGGT 1 cut(s) 601
CviAII CATG 4 cut(s) 154, 169, 363, 592
CviQI GTAC 2 cut(s) 15, 858
DdeI CTNAG 1 cut(s) 637
DpnI GATC 4 cut(s) 249, 507, 626, 686
DpnII GATC 4 cut(s) 247, 505, 624, 684
DraI TTTAAA 1 cut(s) 775
DraIII CACNNNGTG 1 cut(s) 172
DriI GACNNNNNGTC 1 cut(s) 358
EaeI YGGCCR 2 cut(s) 798, 825
Eam1104I CTCTTC 3 cut(s) 236, 864, 900
Eam1105I GACNNNNNGTC 1 cut(s) 358
EarI CTCTTC 3 cut(s) 236, 864, 900
Eco130I CCWWGG 1 cut(s) 611
Eco24I GRGCYC 1 cut(s) 165
Eco47I GGWCC 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 444
EcoRI GAATTC 1 cut(s) 757
EcoRII CCWGG 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 611
EcoT38I GRGCYC 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 611
FaeI CATG 4 cut(s) 157, 172, 366, 595
FatI CATG 4 cut(s) 153, 168, 362, 591
FauI CCCGC 1 cut(s) 133
Fnu4HI GCNGC 3 cut(s) 69, 724, 933
FokI GGATG 1 cut(s) 56
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 3 cut(s) 69, 724, 933
FspBI CTAG 4 cut(s) 503, 647, 861, 955
GluI GCNGC 3 cut(s) 69, 724, 933
HaeIII GGCC 2 cut(s) 800, 827
HapII CCGG 2 cut(s) 602, 801
Hin1II CATG 4 cut(s) 157, 172, 366, 595
HinfI GANTC 1 cut(s) 234
HpaII CCGG 2 cut(s) 602, 801
HphI GGTGA 5 cut(s) 17, 205, 469, 546, 600
Hpy188I TCNGA 4 cut(s) 252, 522, 624, 840
Hpy188III TCNNGA 3 cut(s) 238, 350, 682
HpyAV CCTTC 6 cut(s) 139, 298, 311, 579, 760, 884
HpyCH4III ACNGT 3 cut(s) 130, 538, 914
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 1 cut(s) 269
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 77, 86, 107
HpyF3I CTNAG 1 cut(s) 637
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 4 cut(s) 157, 172, 366, 595
Kzo9I GATC 4 cut(s) 247, 505, 624, 684
LmnI GCTCC 3 cut(s) 168, 178, 601
Lsp1109I GCAGC 3 cut(s) 55, 735, 944
LweI GCATC 2 cut(s) 34, 944
MaeI CTAG 4 cut(s) 503, 647, 861, 955
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 4 cut(s) 5, 25, 130, 649
MalI GATC 4 cut(s) 249, 507, 626, 686
MbiI CCGCTC 1 cut(s) 181
MboI GATC 4 cut(s) 247, 505, 624, 684
MboII GAAGA 5 cut(s) 253, 509, 881, 895, 917
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 2 cut(s) 165, 679
MlsI TGGCCA 1 cut(s) 827
MluCI AATT 4 cut(s) 369, 757, 834, 875
MluNI TGGCCA 1 cut(s) 827
MmeI TCCRAC 1 cut(s) 475
MnlI CCTC 9 cut(s) 130, 205, 485, 499, 644, 710, 713, 865, 922
Mox20I TGGCCA 1 cut(s) 827
MscI TGGCCA 1 cut(s) 827
MseI TTAA 1 cut(s) 774
MslI CAYNNNNRTG 2 cut(s) 173, 539
Msp20I TGGCCA 1 cut(s) 827
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 2 cut(s) 602, 801
MspR9I CCNGG 1 cut(s) 423
Mva1269I GAATGC 1 cut(s) 82
MvaI CCWGG 1 cut(s) 423
MwoI GCNNNNNNNGC 4 cut(s) 53, 77, 86, 107
NdeII GATC 4 cut(s) 247, 505, 624, 684
NlaIII CATG 4 cut(s) 157, 172, 366, 595
NlaIV GGNNCC 3 cut(s) 164, 449, 597
NmuCI GTSAC 2 cut(s) 5, 649
NspI RCATGY 1 cut(s) 157
PaqCI CACCTGC 1 cut(s) 488
PciI ACATGT 1 cut(s) 153
PctI GAATGC 1 cut(s) 82
PfeI GAWTC 1 cut(s) 234
PflMI CCANNNNNTGG 1 cut(s) 172
PinAI ACCGGT 1 cut(s) 601
PkrI GCNGC 3 cut(s) 70, 725, 934
PscI ACATGT 1 cut(s) 153
PsiI TTATAA 1 cut(s) 794
Psp6I CCWGG 1 cut(s) 421
PspGI CCWGG 1 cut(s) 421
PspN4I GGNNCC 3 cut(s) 164, 449, 597
PspPI GGNCC 1 cut(s) 448
PsrI GAACNNNNNNTAC 2 cut(s) 803, 835
PsuI RGATCY 1 cut(s) 505
RsaI GTAC 2 cut(s) 16, 859
RsaNI GTAC 2 cut(s) 15, 858
RseI CAYNNNNRTG 2 cut(s) 173, 539
SaqAI TTAA 1 cut(s) 774
SatI GCNGC 3 cut(s) 69, 724, 933
Sau3AI GATC 4 cut(s) 247, 505, 624, 684
Sau96I GGNCC 1 cut(s) 448
ScaI AGTACT 1 cut(s) 859
ScrFI CCNGG 1 cut(s) 423
SduI GDGCHC 2 cut(s) 165, 679
SfaNI GCATC 2 cut(s) 34, 944
SinI GGWCC 1 cut(s) 448
SmiMI CAYNNNNRTG 2 cut(s) 173, 539
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
SpeI ACTAGT 1 cut(s) 860
Sse9I AATT 4 cut(s) 369, 757, 834, 875
SsiI CCGC 4 cut(s) 66, 140, 181, 391
SspMI CTAG 4 cut(s) 503, 647, 861, 955
StyD4I CCNGG 1 cut(s) 421
StyI CCWWGG 1 cut(s) 611
TaaI ACNGT 3 cut(s) 130, 538, 914
TaiI ACGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 923
TasI AATT 4 cut(s) 369, 757, 834, 875
TatI WGTACW 1 cut(s) 857
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 1 cut(s) 774
Tru9I TTAA 1 cut(s) 774
TscAI CASTG 1 cut(s) 733
TseFI GTSAC 2 cut(s) 5, 649
TseI GCWGC 3 cut(s) 68, 723, 932
Tsp45I GTSAC 2 cut(s) 5, 649
TspDTI ATGAA 3 cut(s) 110, 193, 317
TspGWI ACGGA 1 cut(s) 149
TspRI CASTG 1 cut(s) 733
Van91I CCANNNNNTGG 1 cut(s) 172
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 2 cut(s) 369, 757
XceI RCATGY 1 cut(s) 157
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XspI CTAG 4 cut(s) 503, 647, 861, 955
ZrmI AGTACT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.