Rroxscaffold_3G00262920

Ig-like domain from next to BRCA1 gene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007181
Physical Location & Seq
Forward (+)
56887248 .. 56895597
8350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262920.1

Sequence Viewer

Length: 2967 bp
ATGGCTTCGTCTACTATGGTGATCAAGGTCAAATATGGGGATACACTGAGGCGTTTCAATGCTCGTGTTGATGAGAATGATCAACTGGATCTTGACATGGGTGGACTGCGGTTCAAAATATATAGTCTCTTCAATTTTCAGCTTGATGCTGACATCACTATGACCTATATTGACGAAGATGGTGATATAGTGACTCTGGTTGATGATGAGGATTTGCGTGATGCTATGAGGCAGAATATGAAGTTCTTGAGGATTGATGTACATGAGAAAACTGACAAATCTGAGAAATCTGATGCCAAATCAAGTGGAAGTTCTACCCCTGTAAGATATCCCTTGCCAGATATCACCCCTGAGCCTATCCGTGAACTAATCTCCAGGGTTCCTCGTGACTTGACTCCAAACGCCCTTGGCCCAGGGTTTGCGGATCTTGTTGACTGCTTTTCGAAGATGGGAATGTCCTATTTACTTCAGGCTGGTGGAGTTTCAGGCGTCAAGAATGATTCCTCTGAGAAACCCTTAGCTCCATCTGCCAATGATATGAAGGATGATGGTAAGTCAGAAACCATCTTAAAGTCTACCACTGAGGAATCTAGCTCTAAAAACAGTCAGGCAAAGGATGCTGTAAATGTGACCAAGGATGTGGGAATGTCGAATCCACCTCAGAATGCTCCAGTTGATCTCAATGTTCTTCCTGCTGATTCTAATCCATCTGGACCCACACCCATCAATGTTACACCAGTTGGAGCAAGTCTACATACTGCTGAGGAGAGGAAGGAGTCAGAGAAGGTTACCAGTGGTCAGGTCAAGGGCAAGTCTCTTGGTTGTGGTGGATCTACAAGTTCTGCTATCCCTGCATTGAATGTTAATGACATGAGCAATAATCATTTCAATCAATGTCCATTTTCAGAGACTCCTATTTTGAATGACTCGGCTCCTGTTGCATATCGTCGTCTTCATCCTTTTAAAAGGAATCATTCTGAAGCAATGGGTGGTATGTTCCACACTGGTGTTCGCTGTGATGGATGTGGTTGTCATCCAATAATTGGACCCCGGTTCAAGTCCGTAGTGAAAGCAGATTATGACCTCTGCCGTATCTGTTTCTCGAGTATTGGTAATGTGGCTGAGTACATTAGAATTGACCACCCTTTGTCTTATCGCCATCCACGTCCTTTCAAGGGGATGCATGAACAACCTCCATGGATGGGTCCTTCAGCATTGCCAAAGATACTGAGAAATTGTAGCGCGAAGCCTGGGCGACCTAAGCTTGACAGCCGCTTCGTTTTGGATGTCAATGTAATGGATGGTACCTTAATGGCCCCATCTACTCCATTTACCAAGATTTGGCGGATGCGCAACAATGGGGGTTTGATCTGGCCTAAAGGAACACAACTCATGTGGATTGGTGGAGACAGGTTTAGCAAATCAGATTCAGTTGAGATAGAGATTCCTGTGCGTGGTGTGTTGGCTGAAAATGAACTTGATATTGCTGTTGATTTTACTGCGCCTGAGTCACCTGGACGGTACATCTCATATTGGAGGATGGCTTCTCCATCTGGTCAGAAGTTTGGGCAACGTGTTTGGGTTCTGATTCAGGTGGATACTTCCCTCAAGGATTCACTCTTTGAGAGCTTTCAAGGTTTGAACCTGAACCTGCCCCCTGAAAACGTGGTTAACTTTCCTGAGCAAATGAACTTTCCTCAGCAACATGTGGGTAGTGATTTTGTCCAGCCTTCTAGCTCTAACTCAGTGAAGGAACCAGCAATTCCCATTCCCGAGAAGCAGCCTGAAAATGAGCAGGATCTCAACTTCCCTATAAATGATAGCTTGCTGGTTGGCCACAGTATTCCTGCCCCTACTACCCCAGTGGCTTCTTCAACTGTGTCATACCCCACTGTCGACCAGAGGACGAGCTCAAAAAATCCTGTGGAGGACACCCTCCTTAAGGAGCTTGAGGACATGGGATTTAAGCAGGTGAATTTGAACAAGGAAATTCTGCGCAGGAATGAGTACAACCTGGAGCAGTCTGTGGATGATCTCTGTGAGGTTGCTGAATGGGATCCTATTCTTGAAGAGCTGCAGGAAATGGGCTTCAGTGATACAGAGATGAACAAGAAGCTGCTTGCGAAGAACAATGGAAGCATCAAGCGCGTGGTCATGGATCTTATCAATGGGGACAAGTTTATTGGTGCCTGCATGGAGCCAGTAATGGTGATAGTTACTGAGCTTTTATTGGGTGGGACATTGCGCAAGTATTGTCTTAACATGCGGCCAAGGTGCTTGGACACACGAGTTGCTGTTGGTTTTGCCCTAGATATTGCTCGTGCAATGGAATGCTTACACTCTCATGGGATCATACACCGTGACTTGAAGCCTGAGAACTTGCTCTTGACTGCAGACCACAAAACTGTTAAACTAGCAGATTTTGGGTTAGCAAGAGAAGAGTCATTAACAGAGATGATGACTGCCGAAACTGGAACATACCGTTGGATGGCTCCAGAGTTGTACAGTACTGTTACGTTAAGGCAGGGAGAGAAGAAGCATTACAACCATAAAGTGGATGCCTATAGCTTTGCAATTGTTTTGTGGGAACTCTTACACAATAAATTGCCTTTTGAAGGCATGTCAAATCTCCAGGCAGCATATGCAGCGGCTTTTAAAAATGTGAGGCCCAGTGCTGACAACCTCCCAGAGGAGTTGAATCTCATCCTAACTTCGTGCTGGCAGGAGGACGCAAATGCTCGACCTAATTTCAGTCAGATAATTCAAATGCTACTAGATTATCTTTACACCATAGCTCCTCCTGTACCTGCGATTCCTTCTCGGATTTTCACCGAGAACACTGTCTTGCCTCCAGAGTCCCCTGGTACAAGCTCTTTGATGGCAGTACGTGATGATGGGGAGACACCCAAAGCACAAACGGAAGATGAGCCAAAAGGATTTTTCTTCTGCTTTAAACAGTGCTATTGA

Protein Analysis

988

Amino Acids

109.85

Weight (kDa)

5.3

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 6 - 89 2.3e-09 PB1 domain
ZZ PF00569 334 - 369 4.7e-09 Zinc finger, ZZ type
N_BRCA1_IG PF16158 431 - 532 4.3e-33 Ig-like domain from next to BRCA1 gene
UBA_NBR1_C PF24932 643 - 682 4e-23 Next to BRCA1 gene 1 protein-like, C-terminal UBA domain
UBA_NBR1_C PF24932 685 - 722 5.5e-11 Next to BRCA1 gene 1 protein-like, C-terminal UBA domain
PK_Tyr_Ser-Thr PF07714 726 - 924 6e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 735 - 923 1.8e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1960
Acc16I TGCGCA 3 cut(s) 1352, 1997, 2246
Acc36I ACCTGC 3 cut(s) 1659, 1960, 2815
Acc65I GGTACC 1 cut(s) 1304
AccB1I GGYRCC 2 cut(s) 1304, 2186
AccB7I CCANNNNNTGG 3 cut(s) 1043, 1341, 2554
AccI GTMKAC 4 cut(s) 11, 575, 751, 1896
AccII CGCG 2 cut(s) 1244, 2148
AciI CCGC 6 cut(s) 109, 422, 1273, 1345, 2266, 2648
AclWI GGATC 8 cut(s) 96, 432, 838, 1806, 2051, 2064, 2166, 2357
AcoI YGGCCR 2 cut(s) 1834, 2267
AcsI RAATTY 2 cut(s) 1975, 1989
AcuI CTGAAG 4 cut(s) 452, 999, 1194, 2074
AcyI GRCGYC 1 cut(s) 489
AflII CTTAAG 1 cut(s) 1940
AflIII ACRYGT 2 cut(s) 1573, 1705
AjiI CACGTC 1 cut(s) 1166
AjnI CCWGG 7 cut(s) 374, 412, 1249, 1513, 2013, 2631, 2860
AjuI GAANNNNNNNTTGG 2 cut(s) 2467, 2499
AleI CACNNNNGTG 1 cut(s) 1005
Alw21I GWGCWC 1 cut(s) 1913
Alw26I GTCTC 5 cut(s) 131, 819, 902, 1401, 2894
AlwI GGATC 8 cut(s) 96, 432, 838, 1806, 2051, 2064, 2166, 2357
Ama87I CYCGRG 2 cut(s) 1102, 1772
AoxI GGCC 6 cut(s) 409, 1314, 1373, 1834, 2267, 2666
ApeKI GCWGC 5 cut(s) 1780, 2074, 2116, 2636, 2645
ApoI RAATTY 2 cut(s) 1975, 1989
ArsI GACNNNNNNTTYG 4 cut(s) 901, 933, 1259, 1291
Asp718I GGTACC 1 cut(s) 1304
AspLEI GCGC 6 cut(s) 1244, 1353, 1504, 1998, 2148, 2247
AspS9I GGNCC 6 cut(s) 410, 713, 1046, 1205, 1315, 2667
AsuC2I CCSGG 1 cut(s) 1051
AsuHPI GGTGA 7 cut(s) 31, 194, 337, 1503, 1984, 2221, 2821
AsuII TTCGAA 1 cut(s) 443
AvaI CYCGRG 2 cut(s) 1102, 1772
AvaII GGWCC 3 cut(s) 713, 1046, 1205
BalI TGGCCA 1 cut(s) 1836
BamHI GGATCC 1 cut(s) 2056
BanI GGYRCC 2 cut(s) 1304, 2186
BanII GRGCYC 1 cut(s) 1913
BarI GAAGNNNNNNTAC 2 cut(s) 2525, 2557
BauI CACGAG 4 cut(s) 63, 384, 2286, 2319
BbsI GAAGAC 1 cut(s) 944
Bbv12I GWGCWC 1 cut(s) 1913
BbvCI CCTCAGC 2 cut(s) 762, 1698
BbvI GCAGC 5 cut(s) 1792, 2061, 2103, 2648, 2657
BceAI ACGGC 1 cut(s) 1074
BciT130I CCWGG 7 cut(s) 376, 414, 1251, 1515, 2015, 2633, 2862
BciVI GTATCC 2 cut(s) 34, 1591
BclI TGATCA 2 cut(s) 21, 79
BcnI CCSGG 1 cut(s) 1051
BcoDI GTCTC 5 cut(s) 131, 819, 902, 1401, 2894
BfaI CTAG 5 cut(s) 591, 1734, 2309, 2414, 2774
BfmI CTRYAG 3 cut(s) 2075, 2391, 2563
BfrI CTTAAG 1 cut(s) 1940
BfuAI ACCTGC 3 cut(s) 1659, 1960, 2815
BfuI GTATCC 2 cut(s) 34, 1591
BisI GCNGC 8 cut(s) 1273, 1781, 2075, 2117, 2267, 2637, 2646, 2649
BlsI GCNGC 8 cut(s) 1274, 1782, 2076, 2118, 2268, 2638, 2647, 2650
BmcAI AGTACT 1 cut(s) 2509
Bme1390I CCNGG 8 cut(s) 376, 414, 1051, 1251, 1515, 2015, 2633, 2862
Bme18I GGWCC 3 cut(s) 713, 1046, 1205
BmeT110I CYCGRG 2 cut(s) 1102, 1772
BmgBI CACGTC 1 cut(s) 1166
BmgT120I GGNCC 6 cut(s) 410, 713, 1046, 1205, 1315, 2667
BmrFI CCNGG 8 cut(s) 376, 414, 1051, 1251, 1515, 2015, 2633, 2862
BmrI ACTGGG 2 cut(s) 1856, 2664
BmsI GCATC 8 cut(s) 136, 211, 283, 607, 1170, 1338, 2148, 2548
BmuI ACTGGG 2 cut(s) 1856, 2664
BpiI GAAGAC 1 cut(s) 944
BpmI CTGGAG 6 cut(s) 358, 654, 2036, 2478, 2615, 2835
Bpu10I CCTNAGC 6 cut(s) 351, 517, 762, 1260, 1680, 1698
Bpu14I TTCGAA 1 cut(s) 443
BpuEI CTTGAG 3 cut(s) 268, 1592, 1970
BpuMI CCSGG 1 cut(s) 1051
BsaAI YACGTR 1 cut(s) 2888
BsaBI GATNNNNATC 1 cut(s) 702
BsaHI GRCGYC 1 cut(s) 489
BsaXI ACNNNNNCTCC 2 cut(s) 2779, 2809
Bse1I ACTGG 9 cut(s) 90, 671, 737, 792, 1009, 1862, 2201, 2476, 2670
Bse3DI GCAATG 4 cut(s) 990, 1214, 2240, 2331
Bse8I GATNNNNATC 1 cut(s) 702
BseBI CCWGG 7 cut(s) 376, 414, 1251, 1515, 2015, 2633, 2862
BseJI GATNNNNATC 1 cut(s) 702
BseMI GCAATG 4 cut(s) 990, 1214, 2240, 2331
BseNI ACTGG 9 cut(s) 90, 671, 737, 792, 1009, 1862, 2201, 2476, 2670
BseRI GAGGAG 3 cut(s) 779, 2705, 2787
BseXI GCAGC 5 cut(s) 1792, 2061, 2103, 2648, 2657
Bsh1236I CGCG 2 cut(s) 1244, 2148
BshFI GGCC 6 cut(s) 411, 1316, 1375, 1836, 2269, 2668
BshNI GGYRCC 2 cut(s) 1304, 2186
BsiHKAI GWGCWC 1 cut(s) 1913
BsiHKCI CYCGRG 2 cut(s) 1102, 1772
BsiSI CCGG 1 cut(s) 1051
BslFI GGGAC 3 cut(s) 2186, 2251, 2842
BsmAI GTCTC 5 cut(s) 131, 819, 902, 1401, 2894
BsmFI GGGAC 3 cut(s) 2186, 2251, 2842
BsmI GAATGC 2 cut(s) 670, 2336
BsnI GGCC 6 cut(s) 411, 1316, 1375, 1836, 2269, 2668
BsoBI CYCGRG 2 cut(s) 1102, 1772
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 1 cut(s) 1913
Bsp1407I TGTACA 2 cut(s) 259, 2502
Bsp19I CCATGG 1 cut(s) 1196
BspACI CCGC 6 cut(s) 109, 422, 1273, 1345, 2266, 2648
BspANI GGCC 6 cut(s) 411, 1316, 1375, 1836, 2269, 2668
BspFNI CGCG 2 cut(s) 1244, 2148
BspMAI CTGCAG 2 cut(s) 2079, 2395
BspMI ACCTGC 3 cut(s) 1659, 1960, 2815
BspPI GGATC 8 cut(s) 96, 432, 838, 1806, 2051, 2064, 2166, 2357
BspQI GCTCTTC 1 cut(s) 2064
BspT104I TTCGAA 1 cut(s) 443
BspT107I GGYRCC 2 cut(s) 1304, 2186
BspTI CTTAAG 1 cut(s) 1940
BsrDI GCAATG 4 cut(s) 990, 1214, 2240, 2331
BsrGI TGTACA 2 cut(s) 259, 2502
BsrI ACTGG 9 cut(s) 90, 671, 737, 792, 1009, 1862, 2201, 2476, 2670
BssNI GRCGYC 1 cut(s) 489
BssSI CACGAG 4 cut(s) 63, 384, 2286, 2319
BssT1I CCWWGG 4 cut(s) 406, 633, 1196, 2270
Bst2BI CACGAG 4 cut(s) 63, 384, 2286, 2319
Bst2UI CCWGG 7 cut(s) 376, 414, 1251, 1515, 2015, 2633, 2862
Bst6I CTCTTC 3 cut(s) 134, 2064, 2433
BstACI GRCGYC 1 cut(s) 489
BstAFI CTTAAG 1 cut(s) 1940
BstAPI GCANNNNNTGC 2 cut(s) 617, 2642
BstAUI TGTACA 2 cut(s) 259, 2502
BstBAI YACGTR 1 cut(s) 2888
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 4 cut(s) 1826, 2121, 2191, 2720
BstDSI CCRYGG 1 cut(s) 1196
BstEII GGTNACC 1 cut(s) 787
BstENI CCTNNNNNAGG 3 cut(s) 1940, 2613, 2687
BstFNI CGCG 2 cut(s) 1244, 2148
BstHHI GCGC 6 cut(s) 1244, 1353, 1504, 1998, 2148, 2247
BstMAI GTCTC 5 cut(s) 131, 819, 902, 1401, 2894
BstMWI GCNNNNNNNGC 8 cut(s) 527, 617, 851, 938, 1261, 2145, 2642, 2645
BstNI CCWGG 7 cut(s) 376, 414, 1251, 1515, 2015, 2633, 2862
BstNSI RCATGY 3 cut(s) 1709, 2266, 2623
BstPI GGTNACC 1 cut(s) 787
BstSCI CCNGG 8 cut(s) 374, 412, 1049, 1249, 1513, 2013, 2631, 2860
BstSFI CTRYAG 3 cut(s) 2075, 2391, 2563
BstUI CGCG 2 cut(s) 1244, 2148
BstV1I GCAGC 5 cut(s) 1792, 2061, 2103, 2648, 2657
BstV2I GAAGAC 1 cut(s) 944
BstX2I RGATCY 6 cut(s) 88, 424, 830, 1798, 2056, 2158
BstXI CCANNNNNNTGG 1 cut(s) 640
BstYI RGATCY 6 cut(s) 88, 424, 830, 1798, 2056, 2158
BsuI GTATCC 2 cut(s) 34, 1591
BsuRI GGCC 6 cut(s) 411, 1316, 1375, 1836, 2269, 2668
BtgI CCRYGG 1 cut(s) 1196
BtrI CACGTC 1 cut(s) 1166
BveI ACCTGC 3 cut(s) 1659, 1960, 2815
Cac8I GCNNGC 4 cut(s) 1826, 2121, 2191, 2720
CfoI GCGC 6 cut(s) 1244, 1353, 1504, 1998, 2148, 2247
Cfr13I GGNCC 6 cut(s) 410, 713, 1046, 1205, 1315, 2667
CseI GACGC 2 cut(s) 478, 2738
CspCI CAANNNNNGTGG 4 cut(s) 286, 321, 1376, 1411
DraI TTTAAA 3 cut(s) 964, 2656, 2953
EaeI YGGCCR 2 cut(s) 1834, 2267
Eam1104I CTCTTC 3 cut(s) 134, 2064, 2433
EarI CTCTTC 3 cut(s) 134, 2064, 2433
EciI GGCGGA 1 cut(s) 1360
Ecl136II GAGCTC 1 cut(s) 1911
Eco130I CCWWGG 4 cut(s) 406, 633, 1196, 2270
Eco24I GRGCYC 1 cut(s) 1913
Eco32I GATATC 2 cut(s) 329, 343
Eco47I GGWCC 3 cut(s) 713, 1046, 1205
Eco53kI GAGCTC 1 cut(s) 1911
Eco57I CTGAAG 4 cut(s) 452, 999, 1194, 2074
Eco88I CYCGRG 2 cut(s) 1102, 1772
Eco91I GGTNACC 1 cut(s) 787
EcoICRI GAGCTC 1 cut(s) 1911
EcoNI CCTNNNNNAGG 3 cut(s) 1940, 2613, 2687
EcoO109I RGGNCCY 1 cut(s) 1205
EcoO65I GGTNACC 1 cut(s) 787
EcoRII CCWGG 7 cut(s) 374, 412, 1249, 1513, 2013, 2631, 2860
EcoRV GATATC 2 cut(s) 329, 343
EcoT14I CCWWGG 4 cut(s) 406, 633, 1196, 2270
EcoT22I ATGCAT 1 cut(s) 1185
EcoT38I GRGCYC 1 cut(s) 1913
ErhI CCWWGG 4 cut(s) 406, 633, 1196, 2270
FaqI GGGAC 3 cut(s) 2186, 2251, 2842
FauNDI CATATG 1 cut(s) 2641
FbaI TGATCA 2 cut(s) 21, 79
FblI GTMKAC 4 cut(s) 11, 575, 751, 1896
Fnu4HI GCNGC 8 cut(s) 1273, 1781, 2075, 2117, 2267, 2637, 2646, 2649
FriOI GRGCYC 1 cut(s) 1913
Fsp4HI GCNGC 8 cut(s) 1273, 1781, 2075, 2117, 2267, 2637, 2646, 2649
FspBI CTAG 5 cut(s) 591, 1734, 2309, 2414, 2774
FspI TGCGCA 3 cut(s) 1352, 1997, 2246
GlaI GCGC 6 cut(s) 1243, 1352, 1503, 1997, 2147, 2246
GluI GCNGC 8 cut(s) 1273, 1781, 2075, 2117, 2267, 2637, 2646, 2649
GsuI CTGGAG 6 cut(s) 358, 654, 2036, 2478, 2615, 2835
HaeIII GGCC 6 cut(s) 411, 1316, 1375, 1836, 2269, 2668
HapII CCGG 1 cut(s) 1051
HgaI GACGC 2 cut(s) 478, 2738
HhaI GCGC 6 cut(s) 1244, 1353, 1504, 1998, 2148, 2247
Hin1I GRCGYC 1 cut(s) 489
Hin6I GCGC 6 cut(s) 1242, 1351, 1502, 1996, 2146, 2245
HinP1I GCGC 6 cut(s) 1242, 1351, 1502, 1996, 2146, 2245
HincII GTYRAC 3 cut(s) 433, 1672, 1897
HindII GTYRAC 3 cut(s) 433, 1672, 1897
HindIII AAGCTT 1 cut(s) 1262
HpaI GTTAAC 1 cut(s) 1672
HpaII CCGG 1 cut(s) 1051
HphI GGTGA 7 cut(s) 31, 194, 337, 1503, 1984, 2221, 2821
Hpy166II GTNNAC 8 cut(s) 12, 104, 365, 433, 576, 752, 1672, 1897
Hpy8I GTNNAC 8 cut(s) 12, 104, 365, 433, 576, 752, 1672, 1897
Hpy99I CGWCG 1 cut(s) 951
HpyAV CCTTC 8 cut(s) 535, 766, 778, 1218, 1740, 1744, 2609, 2826
HpyCH4IV ACGT 5 cut(s) 1165, 1573, 1665, 2516, 2887
HpyCH4V TGCA 9 cut(s) 854, 941, 1183, 2077, 2193, 2324, 2393, 2573, 2645
HpyF10VI GCNNNNNNNGC 8 cut(s) 527, 617, 851, 938, 1261, 2145, 2642, 2645
HpySE526I ACGT 5 cut(s) 1165, 1573, 1665, 2516, 2887
Hsp92I GRCGYC 1 cut(s) 489
HspAI GCGC 6 cut(s) 1242, 1351, 1502, 1996, 2146, 2245
KpnI GGTACC 1 cut(s) 1308
Ksp22I TGATCA 2 cut(s) 21, 79
KspAI GTTAAC 1 cut(s) 1672
LguI GCTCTTC 1 cut(s) 2064
LmnI GCTCC 9 cut(s) 526, 673, 743, 937, 1945, 2017, 2197, 2497, 2800
Lsp1109I GCAGC 5 cut(s) 1792, 2061, 2103, 2648, 2657
LweI GCATC 8 cut(s) 136, 211, 283, 607, 1170, 1338, 2148, 2548
MaeI CTAG 5 cut(s) 591, 1734, 2309, 2414, 2774
MaeII ACGT 5 cut(s) 1165, 1573, 1665, 2516, 2887
MaeIII GTNAC 9 cut(s) 190, 386, 628, 730, 787, 1509, 2215, 2360, 2512
MfeI CAATTG 1 cut(s) 2574
MflI RGATCY 6 cut(s) 88, 424, 830, 1798, 2056, 2158
MhlI GDGCHC 1 cut(s) 1913
MlsI TGGCCA 1 cut(s) 1836
MluNI TGGCCA 1 cut(s) 1836
MlyI GAGTC 8 cut(s) 187, 388, 785, 904, 920, 1517, 2450, 2864
MmeI TCCRAC 2 cut(s) 721, 2465
Mox20I TGGCCA 1 cut(s) 1836
Mph1103I ATGCAT 1 cut(s) 1185
MscI TGGCCA 1 cut(s) 1836
MslI CAYNNNNRTG 3 cut(s) 158, 1005, 2343
Msp20I TGGCCA 1 cut(s) 1836
MspA1I CMGCKG 1 cut(s) 2648
MspCI CTTAAG 1 cut(s) 1940
MspI CCGG 1 cut(s) 1051
MspR9I CCNGG 8 cut(s) 376, 414, 1051, 1251, 1515, 2015, 2633, 2862
MunI CAATTG 1 cut(s) 2574
Mva1269I GAATGC 2 cut(s) 670, 2336
MvaI CCWGG 7 cut(s) 376, 414, 1251, 1515, 2015, 2633, 2862
MvnI CGCG 2 cut(s) 1244, 2148
MwoI GCNNNNNNNGC 8 cut(s) 527, 617, 851, 938, 1261, 2145, 2642, 2645
NciI CCSGG 1 cut(s) 1051
NcoI CCATGG 1 cut(s) 1196
NdeI CATATG 1 cut(s) 2641
NmeAIII GCCGAG 1 cut(s) 908
NmuCI GTSAC 5 cut(s) 190, 386, 628, 1509, 2360
NsbI TGCGCA 3 cut(s) 1352, 1997, 2246
NsiI ATGCAT 1 cut(s) 1185
NspI RCATGY 3 cut(s) 1709, 2266, 2623
NspV TTCGAA 1 cut(s) 443
OliI CACNNNNGTG 1 cut(s) 1005
PaeR7I CTCGAG 1 cut(s) 1102
PaqCI CACCTGC 1 cut(s) 1960
PasI CCCWGGG 1 cut(s) 413
PciI ACATGT 1 cut(s) 1705
PciSI GCTCTTC 1 cut(s) 2064
PcsI WCGNNNNNNNCGW 1 cut(s) 1162
PctI GAATGC 2 cut(s) 670, 2336
PflMI CCANNNNNTGG 3 cut(s) 1043, 1341, 2554
PkrI GCNGC 8 cut(s) 1274, 1782, 2076, 2118, 2268, 2638, 2647, 2650
PleI GAGTC 8 cut(s) 187, 388, 784, 904, 920, 1516, 2449, 2863
PpsI GAGTC 8 cut(s) 187, 388, 784, 904, 920, 1516, 2449, 2863
Ppu21I YACGTR 1 cut(s) 2888
PpuMI RGGWCCY 1 cut(s) 1205
PscI ACATGT 1 cut(s) 1705
Psp124BI GAGCTC 1 cut(s) 1913
Psp5II RGGWCCY 1 cut(s) 1205
Psp6I CCWGG 7 cut(s) 374, 412, 1249, 1513, 2013, 2631, 2860
PspEI GGTNACC 1 cut(s) 787
PspGI CCWGG 7 cut(s) 374, 412, 1249, 1513, 2013, 2631, 2860
PspPI GGNCC 6 cut(s) 410, 713, 1046, 1205, 1315, 2667
PspPPI RGGWCCY 1 cut(s) 1205
PstI CTGCAG 2 cut(s) 2079, 2395
PsuI RGATCY 6 cut(s) 88, 424, 830, 1798, 2056, 2158
RseI CAYNNNNRTG 3 cut(s) 158, 1005, 2343
SacI GAGCTC 1 cut(s) 1913
SalI GTCGAC 1 cut(s) 1895
SapI GCTCTTC 1 cut(s) 2064
SatI GCNGC 8 cut(s) 1273, 1781, 2075, 2117, 2267, 2637, 2646, 2649
Sau96I GGNCC 6 cut(s) 410, 713, 1046, 1205, 1315, 2667
ScaI AGTACT 1 cut(s) 2509
SchI GAGTC 8 cut(s) 187, 388, 785, 904, 920, 1517, 2450, 2864
ScrFI CCNGG 8 cut(s) 376, 414, 1051, 1251, 1515, 2015, 2633, 2862
SduI GDGCHC 1 cut(s) 1913
SfaNI GCATC 8 cut(s) 136, 211, 283, 607, 1170, 1338, 2148, 2548
SfcI CTRYAG 3 cut(s) 2075, 2391, 2563
Sfr274I CTCGAG 1 cut(s) 1102
SfuI TTCGAA 1 cut(s) 443
SinI GGWCC 3 cut(s) 713, 1046, 1205
SlaI CTCGAG 1 cut(s) 1102
SmiMI CAYNNNNRTG 3 cut(s) 158, 1005, 2343
SmlI CTYRAG 5 cut(s) 247, 1102, 1607, 1940, 1949
SmoI CTYRAG 5 cut(s) 247, 1102, 1607, 1940, 1949
SsiI CCGC 6 cut(s) 109, 422, 1273, 1345, 2266, 2648
SspMI CTAG 5 cut(s) 591, 1734, 2309, 2414, 2774
SstI GAGCTC 1 cut(s) 1913
StyD4I CCNGG 8 cut(s) 374, 412, 1049, 1249, 1513, 2013, 2631, 2860
StyI CCWWGG 4 cut(s) 406, 633, 1196, 2270
TaiI ACGT 5 cut(s) 1168, 1576, 1668, 2519, 2890
TaqI TCGA 5 cut(s) 443, 650, 1103, 1896, 2740
TatI WGTACW 5 cut(s) 259, 1125, 2007, 2502, 2507
TauI GCSGC 3 cut(s) 1275, 2269, 2651
TseFI GTSAC 5 cut(s) 190, 386, 628, 1509, 2360
TseI GCWGC 5 cut(s) 1780, 2074, 2116, 2636, 2645
Tsp45I GTSAC 5 cut(s) 190, 386, 628, 1509, 2360
TspDTI ATGAA 7 cut(s) 254, 554, 944, 1200, 1488, 1703, 2120
TspGWI ACGGA 3 cut(s) 350, 1051, 2933
Van91I CCANNNNNTGG 3 cut(s) 1043, 1341, 2554
Vha464I CTTAAG 1 cut(s) 1940
VpaK11BI GGWCC 3 cut(s) 713, 1046, 1205
XagI CCTNNNNNAGG 3 cut(s) 1940, 2613, 2687
XapI RAATTY 2 cut(s) 1975, 1989
XceI RCATGY 3 cut(s) 1709, 2266, 2623
XhoI CTCGAG 1 cut(s) 1102
XmiI GTMKAC 4 cut(s) 11, 575, 751, 1896
XspI CTAG 5 cut(s) 591, 1734, 2309, 2414, 2774
ZrmI AGTACT 1 cut(s) 2509
Zsp2I ATGCAT 1 cut(s) 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.