Rroxscaffold_5G00359320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007294
Physical Location & Seq
Reverse (-)
39445227 .. 39447722
2496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00359320.1

Sequence Viewer

Length: 618 bp
ATGCGTCATTTTCGGCCAAACCGCCGTGAGTTACCGTCGTGGGGAGGTTGGGTAGGAGTAGAGAGGAGTCGGCCGGTGCCGGTTGTTCGCTCCCCACGTGGCCGGAAGATGGAGATGGCCGGAGTTGCCGAGAGCGCCGAGGAGAGGGAGAAAACGCGAGAGGAAGGAGAGAGAAGAAGAAAGAAAAAACTTACCCGCCACGAGAATCAAGCCGAGGAGAAAATGAAACAGTTCAAATTGCATCCCACACGATTAGTGTGGACCTTACATGAAGGTGTGGGAAGTGAAAAATTTGTTTTTGGTGCGTCACGCAACCTCCAGGAACAAAAGATAAATCTTCTAGCAGACTTGAGCTGCGAACATCCATGGGCATCGCTAGCAGACTTTGCAAGAAATGAGCAAGAAATGCATGAGGTAGGAAACGAGAAACTCCAACGGGTGCGAACGCTGGGCGCATTCTGGGAGCGCCCGCCTTTTTTGGATCCGATGCTCATACGAGATTATATGCTTTATATCCATAAAGCAATCTGCTCTCTCGAAAAGAGGAAGGCAGCATTGCTCAAAGAACAACAAGAGCTCCTTGTGCAGGCTATCATATATGGGCGACCGTCAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

24.12

Weight (kDa)

9.82

Isoelectric Point (pI)

60.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 76
AccII CGCG 1 cut(s) 157
AciI CCGC 3 cut(s) 22, 196, 470
AclWI GGATC 2 cut(s) 476, 489
AcoI YGGCCR 4 cut(s) 14, 71, 100, 117
AcsI RAATTY 1 cut(s) 290
AcvI CACGTG 1 cut(s) 98
AfiI CCNNNNNNNGG 4 cut(s) 41, 109, 144, 586
AgsI TTSAA 1 cut(s) 235
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 2 cut(s) 354, 577
AluI AGCT 2 cut(s) 354, 577
Alw21I GWGCWC 1 cut(s) 579
AlwI GGATC 2 cut(s) 476, 489
AoxI GGCC 4 cut(s) 14, 71, 100, 117
ApeKI GCWGC 2 cut(s) 354, 551
ApoI RAATTY 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 230
AspLEI GCGC 3 cut(s) 137, 455, 468
AspS9I GGNCC 1 cut(s) 261
AsuNHI GCTAGC 1 cut(s) 376
AvaII GGWCC 1 cut(s) 261
BamHI GGATCC 1 cut(s) 481
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 1 cut(s) 579
BauI CACGAG 1 cut(s) 200
BbrPI CACGTG 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 579
BbvI GCAGC 2 cut(s) 341, 563
BccI CCATC 2 cut(s) 103, 109
BceAI ACGGC 1 cut(s) 9
BciT130I CCWGG 1 cut(s) 320
BfaI CTAG 3 cut(s) 341, 377, 616
BfoI RGCGCY 2 cut(s) 138, 469
BisI GCNGC 2 cut(s) 355, 552
BlsI GCNGC 2 cut(s) 356, 553
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 261
BmiI GGNNCC 2 cut(s) 78, 483
BmrFI CCNGG 1 cut(s) 320
BmsI GCATC 3 cut(s) 250, 380, 477
BmtI GCTAGC 1 cut(s) 380
BpmI CTGGAG 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 370
BsaAI YACGTR 1 cut(s) 98
BsaJI CCNNGG 3 cut(s) 138, 213, 365
BsaXI ACNNNNNCTCC 6 cut(s) 36, 66, 300, 330, 561, 591
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 109, 144, 586
Bse118I RCCGGY 2 cut(s) 73, 79
Bse3DI GCAATG 1 cut(s) 554
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 3 cut(s) 138, 213, 365
BseGI GGATG 2 cut(s) 241, 361
BseLI CCNNNNNNNGG 4 cut(s) 41, 109, 144, 586
BseMI GCAATG 1 cut(s) 554
BseRI GAGGAG 3 cut(s) 79, 155, 230
BseX3I CGGCCG 1 cut(s) 71
BseXI GCAGC 2 cut(s) 341, 563
BseYI CCCAGC 1 cut(s) 448
BsgI GTGCAG 1 cut(s) 605
Bsh1236I CGCG 1 cut(s) 157
Bsh1285I CGRYCG 2 cut(s) 74, 608
BshFI GGCC 4 cut(s) 16, 73, 102, 119
BshNI GGYRCC 1 cut(s) 76
BsiEI CGRYCG 2 cut(s) 74, 608
BsiHKAI GWGCWC 1 cut(s) 579
BsiSI CCGG 4 cut(s) 74, 80, 103, 120
BslI CCNNNNNNNGG 4 cut(s) 41, 109, 144, 586
BsmI GAATGC 1 cut(s) 455
BsnI GGCC 4 cut(s) 16, 73, 102, 119
Bsp1286I GDGCHC 1 cut(s) 579
Bsp143I GATC 1 cut(s) 481
Bsp19I CCATGG 1 cut(s) 365
BspACI CCGC 3 cut(s) 22, 196, 470
BspANI GGCC 4 cut(s) 16, 73, 102, 119
BspFNI CGCG 1 cut(s) 157
BspLI GGNNCC 2 cut(s) 78, 483
BspOI GCTAGC 1 cut(s) 380
BspPI GGATC 2 cut(s) 476, 489
BspT107I GGYRCC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 554
BsrFI RCCGGY 2 cut(s) 73, 79
BssAI RCCGGY 2 cut(s) 73, 79
BssECI CCNNGG 3 cut(s) 138, 213, 365
BssMI GATC 1 cut(s) 481
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 3 cut(s) 36, 231, 609
BstAPI GCANNNNNTGC 2 cut(s) 386, 406
BstBAI YACGTR 1 cut(s) 98
BstC8I GCNNGC 3 cut(s) 378, 470, 588
BstDSI CCRYGG 1 cut(s) 365
BstENI CCTNNNNNAGG 1 cut(s) 584
BstF5I GGATG 2 cut(s) 241, 361
BstFNI CGCG 1 cut(s) 157
BstH2I RGCGCY 2 cut(s) 138, 469
BstHHI GCGC 3 cut(s) 137, 455, 468
BstKTI GATC 1 cut(s) 484
BstMBI GATC 1 cut(s) 481
BstMCI CGRYCG 2 cut(s) 74, 608
BstMWI GCNNNNNNNGC 6 cut(s) 125, 134, 377, 386, 406, 583
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 318
BstUI CGCG 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 341, 563
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BstZI CGGCCG 1 cut(s) 71
BsuRI GGCC 4 cut(s) 16, 73, 102, 119
BtgI CCRYGG 1 cut(s) 365
BtgZI GCGATG 1 cut(s) 357
BtsCI GGATG 2 cut(s) 241, 361
Cac8I GCNNGC 3 cut(s) 378, 470, 588
CfoI GCGC 3 cut(s) 137, 455, 468
Cfr10I RCCGGY 2 cut(s) 73, 79
Cfr13I GGNCC 1 cut(s) 261
CseI GACGC 1 cut(s) 294
CviAII CATG 3 cut(s) 269, 366, 410
CviJI RGCY 8 cut(s) 16, 73, 102, 119, 212, 354, 577, 590
CviKI_1 RGCY 8 cut(s) 16, 73, 102, 119, 212, 354, 577, 590
DpnI GATC 1 cut(s) 483
DpnII GATC 1 cut(s) 481
EaeI YGGCCR 4 cut(s) 14, 71, 100, 117
EagI CGGCCG 1 cut(s) 71
Ecl136II GAGCTC 1 cut(s) 577
EclXI CGGCCG 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 365
Eco24I GRGCYC 1 cut(s) 579
Eco47I GGWCC 1 cut(s) 261
Eco52I CGGCCG 1 cut(s) 71
Eco53kI GAGCTC 1 cut(s) 577
Eco72I CACGTG 1 cut(s) 98
EcoICRI GAGCTC 1 cut(s) 577
EcoNI CCTNNNNNAGG 1 cut(s) 584
EcoRII CCWGG 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 365
EcoT22I ATGCAT 1 cut(s) 411
EcoT38I GRGCYC 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 365
FaeI CATG 3 cut(s) 272, 369, 413
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FatI CATG 3 cut(s) 268, 365, 409
FauI CCCGC 2 cut(s) 203, 477
Fnu4HI GCNGC 2 cut(s) 355, 552
FokI GGATG 2 cut(s) 228, 348
FriOI GRGCYC 1 cut(s) 579
Fsp4HI GCNGC 2 cut(s) 355, 552
FspBI CTAG 3 cut(s) 341, 377, 616
GlaI GCGC 3 cut(s) 136, 454, 467
GluI GCNGC 2 cut(s) 355, 552
GsaI CCCAGC 1 cut(s) 452
GsuI CTGGAG 1 cut(s) 302
HaeII RGCGCY 2 cut(s) 138, 469
HaeIII GGCC 4 cut(s) 16, 73, 102, 119
HapII CCGG 4 cut(s) 74, 80, 103, 120
HgaI GACGC 1 cut(s) 294
HhaI GCGC 3 cut(s) 137, 455, 468
Hin1II CATG 3 cut(s) 272, 369, 413
Hin6I GCGC 3 cut(s) 135, 453, 466
HinP1I GCGC 3 cut(s) 135, 453, 466
HinfI GANTC 2 cut(s) 67, 205
HpaII CCGG 4 cut(s) 74, 80, 103, 120
Hpy166II GTNNAC 1 cut(s) 261
Hpy188I TCNGA 1 cut(s) 486
Hpy188III TCNNGA 1 cut(s) 536
Hpy8I GTNNAC 1 cut(s) 261
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 3 cut(s) 158, 266, 541
HpyCH4III ACNGT 3 cut(s) 36, 231, 609
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 4 cut(s) 241, 389, 409, 586
HpyF10VI GCNNNNNNNGC 6 cut(s) 125, 134, 377, 386, 406, 583
HpySE526I ACGT 1 cut(s) 97
Hsp92II CATG 3 cut(s) 272, 369, 413
HspAI GCGC 3 cut(s) 135, 453, 466
Kzo9I GATC 1 cut(s) 481
LmnI GCTCC 3 cut(s) 95, 463, 582
LpnPI CCDG 9 cut(s) 87, 93, 116, 133, 305, 332, 434, 445, 572
Lsp1109I GCAGC 2 cut(s) 341, 563
LweI GCATC 3 cut(s) 250, 380, 477
MaeI CTAG 3 cut(s) 341, 377, 616
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 2 cut(s) 30, 306
MalI GATC 1 cut(s) 483
MboI GATC 1 cut(s) 481
MboII GAAGA 4 cut(s) 118, 186, 189, 329
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 1 cut(s) 579
MluCI AATT 2 cut(s) 236, 290
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 1 cut(s) 457
MnlI CCTC 9 cut(s) 38, 57, 133, 138, 154, 208, 326, 406, 537
Mph1103I ATGCAT 1 cut(s) 411
MroXI GAANNNNTTC 1 cut(s) 230
MslI CAYNNNNRTG 1 cut(s) 273
MspI CCGG 4 cut(s) 74, 80, 103, 120
MspR9I CCNGG 1 cut(s) 320
Mva1269I GAATGC 1 cut(s) 455
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 1 cut(s) 157
MwoI GCNNNNNNNGC 6 cut(s) 125, 134, 377, 386, 406, 583
NcoI CCATGG 1 cut(s) 365
NdeII GATC 1 cut(s) 481
NheI GCTAGC 1 cut(s) 376
NlaIII CATG 3 cut(s) 272, 369, 413
NlaIV GGNNCC 2 cut(s) 78, 483
NmeAIII GCCGAG 3 cut(s) 154, 163, 238
NmuCI GTSAC 1 cut(s) 306
NsiI ATGCAT 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 94
PctI GAATGC 1 cut(s) 455
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 1 cut(s) 205
PfoI TCCNGGA 1 cut(s) 318
PkrI GCNGC 2 cut(s) 356, 553
PleI GAGTC 1 cut(s) 75
PmaCI CACGTG 1 cut(s) 98
PmlI CACGTG 1 cut(s) 98
PpsI GAGTC 1 cut(s) 75
Ppu21I YACGTR 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 579
Psp6I CCWGG 1 cut(s) 318
PspCI CACGTG 1 cut(s) 98
PspFI CCCAGC 1 cut(s) 448
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 78, 483
PspPI GGNCC 1 cut(s) 261
PsuI RGATCY 1 cut(s) 481
RseI CAYNNNNRTG 1 cut(s) 273
SacI GAGCTC 1 cut(s) 579
SatI GCNGC 2 cut(s) 355, 552
Sau3AI GATC 1 cut(s) 481
Sau96I GGNCC 1 cut(s) 261
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 1 cut(s) 579
SetI ASST 8 cut(s) 49, 100, 266, 277, 318, 356, 417, 579
SfaNI GCATC 3 cut(s) 250, 380, 477
SinI GGWCC 1 cut(s) 261
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
Sse9I AATT 2 cut(s) 236, 290
SsiI CCGC 3 cut(s) 22, 196, 470
SspMI CTAG 3 cut(s) 341, 377, 616
SstI GAGCTC 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 318
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 3 cut(s) 36, 231, 609
TaiI ACGT 1 cut(s) 100
TaqI TCGA 1 cut(s) 537
TasI AATT 2 cut(s) 236, 290
TfiI GAWTC 1 cut(s) 205
TseFI GTSAC 1 cut(s) 306
TseI GCWGC 2 cut(s) 354, 551
Tsp45I GTSAC 1 cut(s) 306
TspDTI ATGAA 2 cut(s) 239, 285
VpaK11BI GGWCC 1 cut(s) 261
XagI CCTNNNNNAGG 1 cut(s) 584
XapI RAATTY 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 230
XspI CTAG 3 cut(s) 341, 377, 616
Zsp2I ATGCAT 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.