Rroxscaffold_5G00372950

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017003
Physical Location & Seq
Reverse (-)
54229940 .. 54235683
5744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00372950.1

Sequence Viewer

Length: 483 bp
ATGATCCTTGGCAAAAACCGAACCTTCGACGCCGGTTTGGTGGTCCGAGACGGCCGAAATCGGAAGATATCGACGTTGACTCAAACTGCTACGAGAAATTCGATAGCCATTGTGAAATACCATTCTCTCGGCAAGCTGATTTGCATACCCCACACACAAAGCCTTCCTCAAGACAGCATCATTTTCCGGGAGGATGACCAACTTGATGAAATGTTCATTATCACTCATGGCGGGGTAAGGACTTCCACCAAAATTAATGGCTTGGACACTACAAGTCTTGAAAAAGGCACTTTTACGGAGAACATGCAACTTTTTGAATACCGGATTTCGAATTCTCCAATCAAATCGTCATCAAGCCCCGCATTACCCTTGTCGGAGAAGACCGTCGTGGCCCGGACGGATGTTCAAGTGTTCATCATAAGAGCCGAAATTCTTGTGGCTCTTCATCCGAGAGGAGCGTGTGTATGGTCCGGCGAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

160

Amino Acids

17.74

Weight (kDa)

9.03

Isoelectric Point (pI)

30.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 53 - 145 1.6e-06 Cyclic nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 231, 360
AcoI YGGCCR 1 cut(s) 52
AcsI RAATTY 3 cut(s) 97, 331, 429
AcyI GRCGYC 1 cut(s) 30
AgsI TTSAA 3 cut(s) 281, 317, 407
AluBI AGCT 1 cut(s) 136
AluI AGCT 1 cut(s) 136
Alw26I GTCTC 1 cut(s) 42
AoxI GGCC 2 cut(s) 52, 390
ApoI RAATTY 3 cut(s) 97, 331, 429
AseI ATTAAT 1 cut(s) 255
AspS9I GGNCC 3 cut(s) 43, 391, 468
AsuC2I CCSGG 2 cut(s) 188, 394
AsuII TTCGAA 1 cut(s) 329
AvaII GGWCC 2 cut(s) 43, 468
BbsI GAAGAC 1 cut(s) 386
BceAI ACGGC 1 cut(s) 67
BcnI CCSGG 2 cut(s) 188, 394
BcoDI GTCTC 1 cut(s) 42
Bme1390I CCNGG 2 cut(s) 188, 394
Bme18I GGWCC 2 cut(s) 43, 468
BmgT120I GGNCC 3 cut(s) 43, 391, 468
BmrFI CCNGG 2 cut(s) 188, 394
BmsI GCATC 1 cut(s) 186
BpiI GAAGAC 1 cut(s) 386
Bpu14I TTCGAA 1 cut(s) 329
BpuEI CTTGAG 1 cut(s) 153
BpuMI CCSGG 2 cut(s) 188, 394
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 7
BsaWI WCCGGW 1 cut(s) 321
BsaXI ACNNNNNCTCC 2 cut(s) 447, 477
Bse118I RCCGGY 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 7
BseGI GGATG 3 cut(s) 199, 406, 445
BseRI GAGGAG 1 cut(s) 468
BseX3I CGGCCG 1 cut(s) 52
Bsh1285I CGRYCG 1 cut(s) 55
BshFI GGCC 2 cut(s) 54, 392
BsiEI CGRYCG 1 cut(s) 55
BsiSI CCGG 5 cut(s) 33, 187, 322, 394, 471
BsmAI GTCTC 1 cut(s) 42
BsmBI CGTCTC 1 cut(s) 42
BsnI GGCC 2 cut(s) 54, 392
Bsp119I TTCGAA 1 cut(s) 329
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 2 cut(s) 231, 360
BspANI GGCC 2 cut(s) 54, 392
BspQI GCTCTTC 1 cut(s) 447
BspT104I TTCGAA 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 1 cut(s) 7
BssMI GATC 1 cut(s) 3
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 1 cut(s) 7
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 1 cut(s) 447
BstACI GRCGYC 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 329
BstC8I GCNNGC 1 cut(s) 134
BstF5I GGATG 3 cut(s) 199, 406, 445
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 1 cut(s) 42
BstMBI GATC 1 cut(s) 3
BstMCI CGRYCG 1 cut(s) 55
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 2 cut(s) 186, 392
BstV2I GAAGAC 1 cut(s) 386
BstZI CGGCCG 1 cut(s) 52
BsuRI GGCC 2 cut(s) 54, 392
BtsCI GGATG 3 cut(s) 199, 406, 445
Cac8I GCNNGC 1 cut(s) 134
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 3 cut(s) 43, 391, 468
CseI GACGC 1 cut(s) 38
CviAII CATG 2 cut(s) 227, 304
CviJI RGCY 9 cut(s) 54, 107, 136, 162, 261, 357, 392, 425, 440
CviKI_1 RGCY 9 cut(s) 54, 107, 136, 162, 261, 357, 392, 425, 440
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
EaeI YGGCCR 1 cut(s) 52
EagI CGGCCG 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
EclXI CGGCCG 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 7
Eco32I GATATC 1 cut(s) 69
Eco47I GGWCC 2 cut(s) 43, 468
Eco52I CGGCCG 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 331
EcoRV GATATC 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 7
ErhI CCWWGG 1 cut(s) 7
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 2 cut(s) 230, 307
FaiI YATR 5 cut(s) 146, 228, 305, 419, 466
FatI CATG 2 cut(s) 226, 303
FauI CCCGC 2 cut(s) 224, 367
FokI GGATG 3 cut(s) 206, 413, 432
HaeIII GGCC 2 cut(s) 54, 392
HapII CCGG 5 cut(s) 33, 187, 322, 394, 471
HgaI GACGC 1 cut(s) 38
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 2 cut(s) 230, 307
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HinfI GANTC 1 cut(s) 79
HpaII CCGG 5 cut(s) 33, 187, 322, 394, 471
Hpy166II GTNNAC 1 cut(s) 78
Hpy188I TCNGA 4 cut(s) 47, 63, 376, 450
Hpy188III TCNNGA 2 cut(s) 170, 278
Hpy8I GTNNAC 1 cut(s) 78
Hpy99I CGWCG 3 cut(s) 32, 76, 389
HpyAV CCTTC 2 cut(s) 34, 173
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 1 cut(s) 74
HpyCH4V TGCA 2 cut(s) 144, 307
HpySE526I ACGT 1 cut(s) 74
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 2 cut(s) 230, 307
Kzo9I GATC 1 cut(s) 3
LguI GCTCTTC 1 cut(s) 447
LmnI GCTCC 1 cut(s) 455
LpnPI CCDG 4 cut(s) 46, 200, 335, 407
LweI GCATC 1 cut(s) 186
MaeII ACGT 1 cut(s) 74
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 3 cut(s) 76, 391, 434
MluCI AATT 4 cut(s) 97, 252, 331, 429
MlyI GAGTC 1 cut(s) 73
MmeI TCCRAC 1 cut(s) 354
MnlI CCTC 4 cut(s) 177, 184, 446, 469
MseI TTAA 2 cut(s) 255, 481
MspI CCGG 5 cut(s) 33, 187, 322, 394, 471
MspR9I CCNGG 2 cut(s) 188, 394
NciI CCSGG 2 cut(s) 188, 394
NdeII GATC 1 cut(s) 3
NlaIII CATG 2 cut(s) 230, 307
NmeAIII GCCGAG 1 cut(s) 108
NspI RCATGY 1 cut(s) 307
NspV TTCGAA 1 cut(s) 329
PciSI GCTCTTC 1 cut(s) 447
PcsI WCGNNNNNNNCGW 1 cut(s) 98
PfoI TCCNGGA 1 cut(s) 186
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PshBI ATTAAT 1 cut(s) 255
PspPI GGNCC 3 cut(s) 43, 391, 468
SapI GCTCTTC 1 cut(s) 447
SaqAI TTAA 2 cut(s) 255, 481
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 3 cut(s) 43, 391, 468
SchI GAGTC 1 cut(s) 73
ScrFI CCNGG 2 cut(s) 188, 394
SetI ASST 4 cut(s) 26, 77, 138, 480
SfaNI GCATC 1 cut(s) 186
SfuI TTCGAA 1 cut(s) 329
SinI GGWCC 2 cut(s) 43, 468
SmlI CTYRAG 1 cut(s) 168
SmoI CTYRAG 1 cut(s) 168
Sse9I AATT 4 cut(s) 97, 252, 331, 429
SsiI CCGC 2 cut(s) 231, 360
StyD4I CCNGG 2 cut(s) 186, 392
StyI CCWWGG 1 cut(s) 7
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 1 cut(s) 77
TaqI TCGA 4 cut(s) 27, 71, 101, 329
TasI AATT 4 cut(s) 97, 252, 331, 429
Tru1I TTAA 2 cut(s) 255, 481
Tru9I TTAA 2 cut(s) 255, 481
TspDTI ATGAA 4 cut(s) 205, 222, 403, 434
TspGWI ACGGA 2 cut(s) 311, 413
VpaK11BI GGWCC 2 cut(s) 43, 468
VspI ATTAAT 1 cut(s) 255
XapI RAATTY 3 cut(s) 97, 331, 429
XceI RCATGY 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.