Rw4G026660

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017003
Physical Location & Seq
Reverse (-)
52905292 .. 52910172
4881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026660.1

Sequence Viewer

Length: 1836 bp
ATGACTCTGCAAATCTTGGAAGCAAATTATGCTGATTGCAAAGATGATAACCCTGTTTTGAAAGCAGTGTGTTCTTATTATGAAGTTCTGGGTTGCAAGACTAGAATTCTTGGCCCCAAAGAGCACCGTCTTCAGACAATTAACTACATCTTTCTTGTTTTCTGCTTTATTGCTCTCGCTCTGGATCCTTTATTCTGTTACATCCTTATCGTCAACGACGAGAACAAGTGCCTTAGATTGGACAAAACAGTGAGTACCGTAGCTACTGCTTCGCGTTCTGCCATTGATTTCTTTTATATGTTTCATACCATATGTCAGTACTTCTGTGCTGCCCTCCCTGATGGTACTATTGTTAACGTTACTCCTCATTCATATCCAAAAATCATCCGAAAACATAAATCCTTTCGAAAAGATCATAAACTGGGACCAAAGAGTAAATGGGAATCCTTCTTGTCGTACCTTTTCTTCCCGCTAGTCGACATATTTGTAATTCTTCCACTCCCACAGGTACTGGCTTTTGCCGTCATTCCAAAATTAAATGAGTCAAGGGTTCTTGATGCAACGCAGACACTCAAATCTGTGTTTCTCTTTCAATACGGGCTAAGGGTTATTCGAATCTGCTCCTTGTTGAAAACTGTTACAAGGGCTTCTGTCAATATCCTCTCTGAGACTACGTGGGCTAATCTCTCCTTTTTTCTACTTGCTAGTCATGTGCTTGGAGCTTTTTGGTATCTATTTTCCATAGAAAGGAAAACAACTTGCTGGGTAAAAGTATGTAAAAATCAAGGTGTTACTTGTTCTTTGTATTGCGATGACACCATGGTACTTAACCCACTCCAACTGATCAATGACTACTCTTGCTCTACAAAGACCAATAACACAGCCTTTGATTATGGAATATACCTTCATGCTCTCGAATCGGGTGTCGTTTATTCAGATAATTTTCTACAAAAGATTTCTCTCTGCTTATGGTGGGGACTGCAAAACATAAGCTCCTTTGGTCAAAGTCTCAAAACAAGTACCTATGTGTGGGAGATCTACTTTTCAATTGTCGTTTCGATTTCTGGCTTGGTACTGTTTGCATTATTTATTGGAAATATACAGACGTACTTGAATTCGAAAGCTGAAAGAGTGAAGGAAATGAAATCGAACGGGCAAGAGATTGAATTGTGGATGGACTTTCATTCACTTCCTAAGTGGCTTAAAACTCGGTTCAGGAAGTATCAGAAGTATAGATGGCAAGAAACCAGAGGGGTTGATGTGGGGAAATTCCTTCAAAAACTTCCCAGGGACCTTGGGAGGGATACTAAGTTATATCTTTGTCTCGATGCTCTCAAGAAAGTGTTAGAATCTCATTGCAAAGACGAACGAGTATTAAGAGCAATCTGTGATCATCTGAAGCCAGTATTCTACATAAAGGACAGCATCATTTTCCAGAAGGATGACCAACTTGATGAAATGTTCATTATCACTCATGGCAGGGTAAAGACTTCCACCAAAATTAATAGCTTGGACACTACAAGTCTTGAAAAAGGGCACTTTTACGGAGAACAACTTTTTGAATACTGGATTTCGAATTCTCCAATCAAATCGTCATCAAGCCCTGCATTACCCTTGTCGGAGAAGACCGTCGTGGCCCAGACAGATGTTCAAAAATTCTTGTGGCTCTTCATCCAGGAGAAGCGTGTGTACGGTCCGGCGAGGTTTAATTCAGAGAAATGGAAACCTTGGGCAGCAAAGACTGTACAAGCAGCATTTCGCAGGCACATTGCGGAAAAAAAAAAAAGGGCTTTAGCCGTCGATTTGAACCCGGATAGACAGGAGAGAAGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

611

Amino Acids

70.76

Weight (kDa)

9.21

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 49 - 377 5.8e-09 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 477
AccII CGCG 1 cut(s) 274
AciI CCGC 2 cut(s) 470, 1772
AclI AACGTT 1 cut(s) 357
AclWI GGATC 2 cut(s) 179, 192
AcsI RAATTY 5 cut(s) 105, 1114, 1268, 1576, 1655
AcuI CTGAAG 2 cut(s) 116, 1418
AfiI CCNNNNNNNGG 4 cut(s) 238, 747, 1029, 1300
AjnI CCWGG 2 cut(s) 1286, 1674
AluBI AGCT 5 cut(s) 263, 722, 993, 1124, 1509
AluI AGCT 5 cut(s) 263, 722, 993, 1124, 1509
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 3 cut(s) 662, 1013, 1328
AlwI GGATC 2 cut(s) 179, 192
AlwNI CAGNNNCTG 1 cut(s) 511
AoxI GGCC 2 cut(s) 112, 1635
ApeKI GCWGC 3 cut(s) 329, 1733, 1751
ApoI RAATTY 5 cut(s) 105, 1114, 1268, 1576, 1655
ArsI GACNNNNNNTTYG 2 cut(s) 909, 941
AseI ATTAAT 1 cut(s) 1503
AspS9I GGNCC 5 cut(s) 113, 425, 1291, 1636, 1694
AsuC2I CCSGG 1 cut(s) 1811
AsuII TTCGAA 4 cut(s) 406, 613, 1118, 1574
AvaII GGWCC 3 cut(s) 425, 1291, 1694
BaeGI GKGCMC 1 cut(s) 1539
BaeI ACNNNNGTAYC 2 cut(s) 336, 369
BamHI GGATCC 1 cut(s) 184
BarI GAAGNNNNNNTAC 4 cut(s) 449, 481, 1673, 1705
BbsI GAAGAC 2 cut(s) 122, 1631
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 3 cut(s) 316, 1745, 1763
BccI CCATC 3 cut(s) 335, 1168, 1230
BceAI ACGGC 2 cut(s) 506, 1781
BciT130I CCWGG 2 cut(s) 1288, 1676
BciVI GTATCC 1 cut(s) 1297
BclI TGATCA 2 cut(s) 843, 1390
BcnI CCSGG 1 cut(s) 1811
BcoDI GTCTC 3 cut(s) 662, 1013, 1328
BfaI CTAG 3 cut(s) 102, 473, 705
BfuI GTATCC 1 cut(s) 1297
BglII AGATCT 1 cut(s) 1035
BisI GCNGC 3 cut(s) 330, 1734, 1752
BlsI GCNGC 3 cut(s) 331, 1735, 1753
BmcAI AGTACT 1 cut(s) 320
Bme1390I CCNGG 3 cut(s) 1288, 1676, 1811
Bme18I GGWCC 3 cut(s) 425, 1291, 1694
BmgT120I GGNCC 5 cut(s) 113, 425, 1291, 1636, 1694
BmiI GGNNCC 4 cut(s) 115, 186, 426, 1292
BmrFI CCNGG 3 cut(s) 1288, 1676, 1811
BmrI ACTGGG 1 cut(s) 431
BmsI GCATC 3 cut(s) 547, 1318, 1434
BmuI ACTGGG 1 cut(s) 431
BpiI GAAGAC 2 cut(s) 122, 1631
Bpu10I CCTNAGC 1 cut(s) 602
Bpu14I TTCGAA 4 cut(s) 406, 613, 1118, 1574
BpuEI CTTGAG 1 cut(s) 1319
BpuMI CCSGG 1 cut(s) 1811
BsaAI YACGTR 1 cut(s) 675
BsaJI CCNNGG 5 cut(s) 819, 1286, 1287, 1294, 1727
BsaXI ACNNNNNCTCC 3 cut(s) 977, 1007, 1814
Bsc4I CCNNNNNNNGG 4 cut(s) 238, 747, 1029, 1300
Bse1I ACTGG 4 cut(s) 426, 516, 1403, 1571
Bse3DI GCAATG 2 cut(s) 1354, 1767
BseBI CCWGG 2 cut(s) 1288, 1676
BseDI CCNNGG 5 cut(s) 819, 1286, 1287, 1294, 1727
BseGI GGATG 5 cut(s) 201, 384, 1179, 1447, 1671
BseLI CCNNNNNNNGG 4 cut(s) 238, 747, 1029, 1300
BseMI GCAATG 2 cut(s) 1354, 1767
BseMII CTCAG 1 cut(s) 657
BseNI ACTGG 4 cut(s) 426, 516, 1403, 1571
BseRI GAGGAG 1 cut(s) 354
BseSI GKGCMC 1 cut(s) 1539
BseXI GCAGC 3 cut(s) 316, 1745, 1763
BseYI CCCAGC 1 cut(s) 762
Bsh1236I CGCG 1 cut(s) 274
BshFI GGCC 2 cut(s) 114, 1637
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 2 cut(s) 1697, 1811
BslFI GGGAC 3 cut(s) 438, 990, 1304
BslI CCNNNNNNNGG 4 cut(s) 238, 747, 1029, 1300
BsmAI GTCTC 3 cut(s) 662, 1013, 1328
BsmFI GGGAC 3 cut(s) 438, 990, 1304
BsnI GGCC 2 cut(s) 114, 1637
Bsp119I TTCGAA 4 cut(s) 406, 613, 1118, 1574
Bsp1286I GDGCHC 2 cut(s) 126, 1539
Bsp1407I TGTACA 1 cut(s) 1744
Bsp143I GATC 5 cut(s) 184, 412, 843, 1035, 1390
Bsp19I CCATGG 1 cut(s) 819
BspACI CCGC 2 cut(s) 470, 1772
BspANI GGCC 2 cut(s) 114, 1637
BspCNI CTCAG 1 cut(s) 658
BspFNI CGCG 1 cut(s) 274
BspLI GGNNCC 4 cut(s) 115, 186, 426, 1292
BspPI GGATC 2 cut(s) 179, 192
BspQI GCTCTTC 1 cut(s) 1673
BspT104I TTCGAA 4 cut(s) 406, 613, 1118, 1574
BsrDI GCAATG 2 cut(s) 1354, 1767
BsrGI TGTACA 1 cut(s) 1744
BsrI ACTGG 4 cut(s) 426, 516, 1403, 1571
BssECI CCNNGG 5 cut(s) 819, 1286, 1287, 1294, 1727
BssMI GATC 5 cut(s) 184, 412, 843, 1035, 1390
BssT1I CCWWGG 3 cut(s) 819, 1294, 1727
Bst2UI CCWGG 2 cut(s) 1288, 1676
Bst4CI ACNGT 8 cut(s) 128, 250, 259, 637, 1077, 1630, 1694, 1744
Bst6I CTCTTC 1 cut(s) 1673
BstAPI GCANNNNNTGC 1 cut(s) 29
BstAUI TGTACA 1 cut(s) 1744
BstBAI YACGTR 1 cut(s) 675
BstBI TTCGAA 4 cut(s) 406, 613, 1118, 1574
BstC8I GCNNGC 1 cut(s) 1763
BstDEI CTNAG 5 cut(s) 233, 602, 666, 1194, 1308
BstDSI CCRYGG 1 cut(s) 819
BstF5I GGATG 5 cut(s) 201, 384, 1179, 1447, 1671
BstFNI CGCG 1 cut(s) 274
BstKTI GATC 5 cut(s) 187, 415, 846, 1038, 1393
BstMAI GTCTC 3 cut(s) 662, 1013, 1328
BstMBI GATC 5 cut(s) 184, 412, 843, 1035, 1390
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 2 cut(s) 1288, 1676
BstSCI CCNGG 3 cut(s) 1286, 1674, 1809
BstSLI GKGCMC 1 cut(s) 1539
BstUI CGCG 1 cut(s) 274
BstV1I GCAGC 3 cut(s) 316, 1745, 1763
BstV2I GAAGAC 2 cut(s) 122, 1631
BstX2I RGATCY 2 cut(s) 184, 1035
BstYI RGATCY 2 cut(s) 184, 1035
BsuI GTATCC 1 cut(s) 1297
BsuRI GGCC 2 cut(s) 114, 1637
BtgI CCRYGG 1 cut(s) 819
BtgZI GCGATG 1 cut(s) 825
BtsCI GGATG 5 cut(s) 201, 384, 1179, 1447, 1671
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 2 cut(s) 72, 255
Cac8I GCNNGC 1 cut(s) 1763
CaiI CAGNNNCTG 1 cut(s) 511
Cfr13I GGNCC 5 cut(s) 113, 425, 1291, 1636, 1694
CpoI CGGWCCG 1 cut(s) 1694
CspI CGGWCCG 1 cut(s) 1694
CviAII CATG 4 cut(s) 710, 820, 908, 1475
DdeI CTNAG 5 cut(s) 233, 602, 666, 1194, 1308
DpnI GATC 5 cut(s) 186, 414, 845, 1037, 1392
DpnII GATC 5 cut(s) 184, 412, 843, 1035, 1390
Eam1104I CTCTTC 1 cut(s) 1673
EarI CTCTTC 1 cut(s) 1673
Eco130I CCWWGG 3 cut(s) 819, 1294, 1727
Eco47I GGWCC 3 cut(s) 425, 1291, 1694
Eco57I CTGAAG 2 cut(s) 116, 1418
EcoO109I RGGNCCY 1 cut(s) 1291
EcoRI GAATTC 3 cut(s) 105, 1114, 1576
EcoRII CCWGG 2 cut(s) 1286, 1674
EcoT14I CCWWGG 3 cut(s) 819, 1294, 1727
ErhI CCWWGG 3 cut(s) 819, 1294, 1727
FaeI CATG 4 cut(s) 713, 823, 911, 1478
FaqI GGGAC 3 cut(s) 438, 990, 1304
FatI CATG 4 cut(s) 709, 819, 907, 1474
FauI CCCGC 1 cut(s) 477
FauNDI CATATG 1 cut(s) 311
FbaI TGATCA 2 cut(s) 843, 1390
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 3 cut(s) 330, 1734, 1752
FokI GGATG 5 cut(s) 188, 371, 1186, 1454, 1658
Fsp4HI GCNGC 3 cut(s) 330, 1734, 1752
FspBI CTAG 3 cut(s) 102, 473, 705
GluI GCNGC 3 cut(s) 330, 1734, 1752
GsaI CCCAGC 1 cut(s) 766
HaeIII GGCC 2 cut(s) 114, 1637
HapII CCGG 2 cut(s) 1697, 1811
Hin1II CATG 4 cut(s) 713, 823, 911, 1478
HincII GTYRAC 3 cut(s) 214, 355, 478
HindII GTYRAC 3 cut(s) 214, 355, 478
HinfI GANTC 6 cut(s) 4, 443, 542, 615, 917, 1349
HpaI GTTAAC 1 cut(s) 355
HpaII CCGG 2 cut(s) 1697, 1811
Hpy166II GTNNAC 4 cut(s) 214, 355, 478, 1690
Hpy188I TCNGA 8 cut(s) 135, 389, 667, 937, 1227, 1398, 1621, 1714
Hpy188III TCNNGA 8 cut(s) 182, 554, 914, 1216, 1325, 1336, 1435, 1526
Hpy8I GTNNAC 4 cut(s) 214, 355, 478, 1690
Hpy99I CGWCG 3 cut(s) 221, 1634, 1802
HpyAV CCTTC 6 cut(s) 457, 914, 1129, 1283, 1432, 1821
HpyCH4III ACNGT 8 cut(s) 128, 250, 259, 637, 1077, 1630, 1694, 1744
HpyCH4IV ACGT 3 cut(s) 357, 674, 1106
HpyCH4V TGCA 8 cut(s) 10, 39, 96, 560, 982, 1082, 1359, 1607
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 5 cut(s) 233, 602, 666, 1194, 1308
HpySE526I ACGT 3 cut(s) 357, 674, 1106
Hsp92II CATG 4 cut(s) 713, 823, 911, 1478
Ksp22I TGATCA 2 cut(s) 843, 1390
KspAI GTTAAC 1 cut(s) 355
Kzo9I GATC 5 cut(s) 184, 412, 843, 1035, 1390
LguI GCTCTTC 1 cut(s) 1673
LmnI GCTCC 3 cut(s) 626, 719, 998
Lsp1109I GCAGC 3 cut(s) 316, 1745, 1763
LweI GCATC 3 cut(s) 547, 1318, 1434
MaeI CTAG 3 cut(s) 102, 473, 705
MaeII ACGT 3 cut(s) 357, 674, 1106
MaeIII GTNAC 4 cut(s) 197, 358, 637, 790
MalI GATC 5 cut(s) 186, 414, 845, 1037, 1392
MboI GATC 5 cut(s) 184, 412, 843, 1035, 1390
MboII GAAGA 5 cut(s) 122, 457, 485, 1636, 1660
MfeI CAATTG 1 cut(s) 1047
MflI RGATCY 2 cut(s) 184, 1035
MhlI GDGCHC 2 cut(s) 126, 1539
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 2 cut(s) 862, 1599
MnlI CCTC 6 cut(s) 344, 375, 671, 1244, 1293, 1695
MseI TTAA 8 cut(s) 141, 354, 536, 828, 1203, 1376, 1503, 1707
MspI CCGG 2 cut(s) 1697, 1811
MspR9I CCNGG 3 cut(s) 1288, 1676, 1811
MunI CAATTG 1 cut(s) 1047
MvaI CCWGG 2 cut(s) 1288, 1676
MvnI CGCG 1 cut(s) 274
MwoI GCNNNNNNNGC 1 cut(s) 29
NciI CCSGG 1 cut(s) 1811
NcoI CCATGG 1 cut(s) 819
NdeI CATATG 1 cut(s) 311
NdeII GATC 5 cut(s) 184, 412, 843, 1035, 1390
NlaIII CATG 4 cut(s) 713, 823, 911, 1478
NlaIV GGNNCC 4 cut(s) 115, 186, 426, 1292
NspV TTCGAA 4 cut(s) 406, 613, 1118, 1574
PasI CCCWGGG 1 cut(s) 1287
PciSI GCTCTTC 1 cut(s) 1673
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 4 cut(s) 443, 615, 917, 1349
PfoI TCCNGGA 1 cut(s) 1674
PkrI GCNGC 3 cut(s) 331, 1735, 1753
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
Ppu21I YACGTR 1 cut(s) 675
PpuMI RGGWCCY 1 cut(s) 1291
PshBI ATTAAT 1 cut(s) 1503
Psp1406I AACGTT 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 1291
Psp6I CCWGG 2 cut(s) 1286, 1674
PspFI CCCAGC 1 cut(s) 762
PspGI CCWGG 2 cut(s) 1286, 1674
PspN4I GGNNCC 4 cut(s) 115, 186, 426, 1292
PspPI GGNCC 5 cut(s) 113, 425, 1291, 1636, 1694
PspPPI RGGWCCY 1 cut(s) 1291
PstNI CAGNNNCTG 1 cut(s) 511
PsuI RGATCY 2 cut(s) 184, 1035
Rsr2I CGGWCCG 1 cut(s) 1694
RsrII CGGWCCG 1 cut(s) 1694
SalI GTCGAC 1 cut(s) 476
SapI GCTCTTC 1 cut(s) 1673
SaqAI TTAA 8 cut(s) 141, 354, 536, 828, 1203, 1376, 1503, 1707
SatI GCNGC 3 cut(s) 330, 1734, 1752
Sau3AI GATC 5 cut(s) 184, 412, 843, 1035, 1390
Sau96I GGNCC 5 cut(s) 113, 425, 1291, 1636, 1694
ScaI AGTACT 1 cut(s) 320
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 3 cut(s) 1288, 1676, 1811
SduI GDGCHC 2 cut(s) 126, 1539
SfaNI GCATC 3 cut(s) 547, 1318, 1434
SfuI TTCGAA 4 cut(s) 406, 613, 1118, 1574
SinI GGWCC 3 cut(s) 425, 1291, 1694
SmlI CTYRAG 1 cut(s) 1334
SmoI CTYRAG 1 cut(s) 1334
SsiI CCGC 2 cut(s) 470, 1772
SspMI CTAG 3 cut(s) 102, 473, 705
StyD4I CCNGG 3 cut(s) 1286, 1674, 1809
StyI CCWWGG 3 cut(s) 819, 1294, 1727
TaaI ACNGT 8 cut(s) 128, 250, 259, 637, 1077, 1630, 1694, 1744
TaiI ACGT 3 cut(s) 360, 677, 1109
TatI WGTACW 2 cut(s) 318, 1744
TfiI GAWTC 4 cut(s) 443, 615, 917, 1349
Tru1I TTAA 8 cut(s) 141, 354, 536, 828, 1203, 1376, 1503, 1707
Tru9I TTAA 8 cut(s) 141, 354, 536, 828, 1203, 1376, 1503, 1707
TscAI CASTG 2 cut(s) 72, 255
TseI GCWGC 3 cut(s) 329, 1733, 1751
TspDTI ATGAA 9 cut(s) 96, 293, 360, 896, 1157, 1172, 1453, 1470, 1660
TspGWI ACGGA 1 cut(s) 1560
TspRI CASTG 2 cut(s) 72, 255
VpaK11BI GGWCC 3 cut(s) 425, 1291, 1694
VspI ATTAAT 1 cut(s) 1503
XapI RAATTY 5 cut(s) 105, 1114, 1268, 1576, 1655
XcmI CCANNNNNNNNNTGG 1 cut(s) 435
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 3 cut(s) 102, 473, 705
ZrmI AGTACT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.