Rroxscaffold_6G00395400

Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0017433
Physical Location & Seq
Forward (+)
16428916 .. 16431875
2960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00395400.1

Sequence Viewer

Length: 753 bp
ATGGAGGACAACGCGTCGAGCCCAACCCGACGTTATTTAATCTCGTCCTCAAGGCTATGCACGGTGGCAAAGAGTCCGAAGCCTCTAATGTTGAGTACCATCTTGAAGTTGAAATGGCCTCTCGCTGTTTCGGCTTCAAGCACCGGGTGCTTAGTTCGTTCACGCCTCCCACTCGGCGAATGTCGCATCATCGTTGTAGCACGGTTTAATGAGATTGTCACAAAGCCACTGCTAGAGGGAGCCTTAACTACTTTAAACAATTATTCTGTTCAAGAAGAGGACATTGATATAAGAGGTGATACAACCCACTACGATGTCGTTGCTAATTCAACCGCATCCGGAGTGCTTTCAGCTGGTTTAAAATCAGGTGTTCCATGCATATTCGGTGTCTTAACTTGTGATAATATGGAGCAGGCTTTAAATCGAGGTGGTGGTAAATCTGGAAATAAGGGTGCAGAGACTGCCTTGACAGCTAATTATATGATGAAGTACTTCAGAGAGGATCTGCATAGGCAGCTTCTTAGTACAGATTTTAAGAAGCAAGTCGATGGGCTTGAGATGCTGCAGATGGCACTTCCTAGAAAGAAAATTATTGAAGTTCTGGATATATTGCCTAGGTGGTTTGTTTTACAATTTTGTAAATCGAACACAACATCTCTGTTAAAGGTGCTGGAATTTCTTTATGACCTTTTTGACATGTTTAGGGATGAGGGATACATGTATAGATGCTGGAATTTTATTATCTTGTTGTGA

Protein Analysis

250

Amino Acids

28.1

Weight (kDa)

8.63

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DMRL_synthase PF00885 97 - 164 9e-20 6,7-dimethyl-8-ribityllumazine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 14
AccIII TCCGGA 1 cut(s) 338
AciI CCGC 1 cut(s) 333
AclWI GGATC 1 cut(s) 510
AcsI RAATTY 2 cut(s) 674, 733
AcuI CTGAAG 1 cut(s) 478
AdeI CACNNNGTG 1 cut(s) 147
AfaI GTAC 3 cut(s) 97, 491, 526
AflIII ACRYGT 3 cut(s) 12, 696, 717
AgsI TTSAA 6 cut(s) 106, 112, 138, 272, 330, 596
AhdI GACNNNNNGTC 1 cut(s) 13
AluBI AGCT 3 cut(s) 353, 473, 517
AluI AGCT 3 cut(s) 353, 473, 517
Alw26I GTCTC 1 cut(s) 452
AlwI GGATC 1 cut(s) 510
AlwNI CAGNNNCTG 1 cut(s) 461
Aor13HI TCCGGA 1 cut(s) 338
AoxI GGCC 1 cut(s) 116
ApeKI GCWGC 2 cut(s) 514, 562
ApoI RAATTY 2 cut(s) 674, 733
Asp700I GAANNNNTTC 1 cut(s) 491
AspA2I CCTAGG 1 cut(s) 614
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 1 cut(s) 308
AvrII CCTAGG 1 cut(s) 614
BanII GRGCYC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 526, 549
BccI CCATC 3 cut(s) 107, 542, 562
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciVI GTATCC 1 cut(s) 707
BcnI CCSGG 1 cut(s) 145
BcoDI GTCTC 1 cut(s) 452
BfaI CTAG 3 cut(s) 233, 579, 615
BfmI CTRYAG 1 cut(s) 563
BfuI GTATCC 1 cut(s) 707
BisI GCNGC 2 cut(s) 515, 563
BlnI CCTAGG 1 cut(s) 614
BlsI GCNGC 2 cut(s) 516, 564
BmcAI AGTACT 1 cut(s) 491
Bme1390I CCNGG 1 cut(s) 145
BmeRI GACNNNNNGTC 1 cut(s) 13
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 1 cut(s) 145
BmsI GCATC 4 cut(s) 195, 344, 549, 716
BpuEI CTTGAG 2 cut(s) 34, 575
BpuMI CCSGG 1 cut(s) 145
BsaJI CCNNGG 1 cut(s) 614
BsaWI WCCGGW 1 cut(s) 338
BseAI TCCGGA 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 614
BseGI GGATG 2 cut(s) 335, 712
BseXI GCAGC 2 cut(s) 526, 549
BsgI GTGCAG 1 cut(s) 474
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 1 cut(s) 118
BsiSI CCGG 2 cut(s) 144, 339
BsmAI GTCTC 1 cut(s) 452
BsnI GGCC 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 23
Bsp13I TCCGGA 1 cut(s) 338
Bsp143I GATC 1 cut(s) 502
BspACI CCGC 1 cut(s) 333
BspANI GGCC 1 cut(s) 118
BspEI TCCGGA 1 cut(s) 338
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 1 cut(s) 241
BspMAI CTGCAG 1 cut(s) 567
BspPI GGATC 1 cut(s) 510
BssECI CCNNGG 1 cut(s) 614
BssMI GATC 1 cut(s) 502
BssT1I CCWWGG 1 cut(s) 614
Bst4CI ACNGT 2 cut(s) 64, 204
Bst6I CTCTTC 1 cut(s) 270
BstAPI GCANNNNNTGC 2 cut(s) 147, 461
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 2 cut(s) 151, 521
BstF5I GGATG 2 cut(s) 335, 712
BstFNI CGCG 1 cut(s) 14
BstKTI GATC 1 cut(s) 505
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 1 cut(s) 502
BstMWI GCNNNNNNNGC 7 cut(s) 131, 147, 183, 461, 470, 514, 559
BstNSI RCATGY 2 cut(s) 700, 721
BstSCI CCNGG 1 cut(s) 143
BstSFI CTRYAG 1 cut(s) 563
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 526, 549
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
BsuI GTATCC 1 cut(s) 707
BsuRI GGCC 1 cut(s) 118
BtsCI GGATG 2 cut(s) 335, 712
BtsI GCAGTG 1 cut(s) 227
BtsIMutI CAGTG 1 cut(s) 227
Cac8I GCNNGC 1 cut(s) 414
CaiI CAGNNNCTG 1 cut(s) 461
CseI GACGC 1 cut(s) 3
Csp6I GTAC 3 cut(s) 96, 490, 525
CviAII CATG 3 cut(s) 375, 697, 718
CviQI GTAC 3 cut(s) 96, 490, 525
DdeI CTNAG 2 cut(s) 151, 521
DpnI GATC 1 cut(s) 504
DpnII GATC 1 cut(s) 502
DraI TTTAAA 3 cut(s) 255, 360, 420
DraIII CACNNNGTG 1 cut(s) 147
DriI GACNNNNNGTC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 270
Eam1105I GACNNNNNGTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 614
Eco24I GRGCYC 1 cut(s) 23
Eco57I CTGAAG 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 614
EcoT22I ATGCAT 1 cut(s) 380
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 614
FaeI CATG 3 cut(s) 378, 700, 721
FatI CATG 3 cut(s) 374, 696, 717
Fnu4HI GCNGC 2 cut(s) 515, 563
FokI GGATG 2 cut(s) 322, 719
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 515, 563
FspBI CTAG 3 cut(s) 233, 579, 615
GluI GCNGC 2 cut(s) 515, 563
HaeIII GGCC 1 cut(s) 118
HapII CCGG 2 cut(s) 144, 339
HgaI GACGC 1 cut(s) 3
Hin1II CATG 3 cut(s) 378, 700, 721
HinfI GANTC 1 cut(s) 73
HpaII CCGG 2 cut(s) 144, 339
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 1 cut(s) 161
Hpy188I TCNGA 2 cut(s) 78, 497
Hpy188III TCNNGA 5 cut(s) 103, 272, 339, 441, 602
Hpy8I GTNNAC 1 cut(s) 161
Hpy99I CGWCG 2 cut(s) 19, 33
HpyCH4III ACNGT 2 cut(s) 64, 204
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 5 cut(s) 60, 378, 455, 508, 565
HpyF10VI GCNNNNNNNGC 7 cut(s) 131, 147, 183, 461, 470, 514, 559
HpyF3I CTNAG 2 cut(s) 151, 521
HpySE526I ACGT 1 cut(s) 31
Hsp92II CATG 3 cut(s) 378, 700, 721
Kpn2I TCCGGA 1 cut(s) 338
Kzo9I GATC 1 cut(s) 502
LmnI GCTCC 2 cut(s) 239, 409
LpnPI CCDG 9 cut(s) 157, 339, 351, 352, 398, 426, 587, 656, 715
Lsp1109I GCAGC 2 cut(s) 526, 549
LweI GCATC 4 cut(s) 195, 344, 549, 716
MaeI CTAG 3 cut(s) 233, 579, 615
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 1 cut(s) 217
MalI GATC 1 cut(s) 504
MboI GATC 1 cut(s) 502
MboII GAAGA 1 cut(s) 287
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 7 cut(s) 259, 325, 475, 588, 632, 674, 733
MluI ACGCGT 1 cut(s) 12
MlyI GAGTC 1 cut(s) 82
Mph1103I ATGCAT 1 cut(s) 380
MroI TCCGGA 1 cut(s) 338
MroXI GAANNNNTTC 1 cut(s) 491
MseI TTAA 9 cut(s) 38, 207, 245, 254, 359, 392, 419, 534, 662
MslI CAYNNNNRTG 1 cut(s) 312
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 2 cut(s) 144, 339
MspR9I CCNGG 1 cut(s) 145
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 7 cut(s) 131, 147, 183, 461, 470, 514, 559
NciI CCSGG 1 cut(s) 145
NdeII GATC 1 cut(s) 502
NlaIII CATG 3 cut(s) 378, 700, 721
NlaIV GGNNCC 1 cut(s) 241
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 217
NsiI ATGCAT 1 cut(s) 380
NspI RCATGY 2 cut(s) 700, 721
PciI ACATGT 2 cut(s) 696, 717
PdmI GAANNNNTTC 1 cut(s) 491
PkrI GCNGC 2 cut(s) 516, 564
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
PscI ACATGT 2 cut(s) 696, 717
PspN4I GGNNCC 1 cut(s) 241
PstI CTGCAG 1 cut(s) 567
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 1 cut(s) 502
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 3 cut(s) 97, 491, 526
RsaNI GTAC 3 cut(s) 96, 490, 525
RseI CAYNNNNRTG 1 cut(s) 312
SaqAI TTAA 9 cut(s) 38, 207, 245, 254, 359, 392, 419, 534, 662
SatI GCNGC 2 cut(s) 515, 563
Sau3AI GATC 1 cut(s) 502
ScaI AGTACT 1 cut(s) 491
SchI GAGTC 1 cut(s) 82
ScrFI CCNGG 1 cut(s) 145
SduI GDGCHC 1 cut(s) 23
SfaNI GCATC 4 cut(s) 195, 344, 549, 716
SfcI CTRYAG 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 2 cut(s) 49, 554
SmoI CTYRAG 2 cut(s) 49, 554
Sse9I AATT 7 cut(s) 259, 325, 475, 588, 632, 674, 733
SsiI CCGC 1 cut(s) 333
SspMI CTAG 3 cut(s) 233, 579, 615
StyD4I CCNGG 1 cut(s) 143
StyI CCWWGG 1 cut(s) 614
TaaI ACNGT 2 cut(s) 64, 204
TaiI ACGT 1 cut(s) 34
TaqI TCGA 4 cut(s) 17, 424, 546, 644
TasI AATT 7 cut(s) 259, 325, 475, 588, 632, 674, 733
TatI WGTACW 2 cut(s) 489, 524
Tru1I TTAA 9 cut(s) 38, 207, 245, 254, 359, 392, 419, 534, 662
Tru9I TTAA 9 cut(s) 38, 207, 245, 254, 359, 392, 419, 534, 662
TscAI CASTG 1 cut(s) 234
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 2 cut(s) 514, 562
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 500
TspRI CASTG 1 cut(s) 234
XapI RAATTY 2 cut(s) 674, 733
XceI RCATGY 2 cut(s) 700, 721
XmaJI CCTAGG 1 cut(s) 614
XmnI GAANNNNTTC 1 cut(s) 491
XspI CTAG 3 cut(s) 233, 579, 615
ZrmI AGTACT 1 cut(s) 491
Zsp2I ATGCAT 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.