Rroxscaffold_6G00414690

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011681
Physical Location & Seq
Reverse (-)
36968537 .. 36972294
3758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414690.1

Sequence Viewer

Length: 1629 bp
ATGAGCAGTTGGACGAAGCTGGATGAGGATCTTGGACCGGATCGCGGGGACGATGATTTTTTAACAGATGATGAAGAGAACCAAGCACACAGAAACAACTATGAGCCTTCCGAAGCCGAAACTGAATCTGAAGTAGGCAGACCTGAGGGCATTGCCATTAGTGCACCTAACAATAATGCATGGCCTCAAAGCTACTGGCAGTCCATCGATATGTACACCAATGTCATGACTCCTCCTTCCCTTCCTTCCCTTACTTTTATGCGAGGGCCCAGTTTCAGCTCCTTGTCTTCGTTTGTTAAGTGGCAACAAGCACCTGATGATAATTCTTCACAGCTCAGTATCAAATTACTTGCTTCCGAAGCAAGTTTAGAAAAAGAAGGGCTTGCAGCATCATCTCCCCCAACTCTCTCCGCAAAAAATTCTGTCTGTGAATTGCCACCGCCAAAGGAACATAGTTGTTCATTTTCACAAGCTGTACTCAATGGAATCAATGTCTTGTGTGGGATGGGACTTATTACAACACCATATGCAATAAAAGAAGGAGGGTGGTTGAGCCTCATTTTACTTGCATTGTTTGGTGTGATATGTTGCTATACTGGAATTTTATTAATGCGATGTTTAGAAAGTTGTCCTGGACTCCGAACCTATCCAGATATTGGCCAGGCTGCTTTTGGCTCGACTGGCCGTTTAATTCTATCTATAATTTTGTATCTAGAACTATATGGTGCTTGTGTGGAGTTTTTGACGATGATGGGCGATAACTTAGCGGCAATATTCCCAAACACCCACATAGCATTTGCAGGAATCCATCTTCAAAGTCATGAAATTTTTGCCATCACAGCAACTCTCGTAGTTCTTCCCACTGTATGGCTCCAAAATCTTAGTTTGCTCTCTTACCTCTCAGTTGGAGGAGTAGGTGCGTCGATTCTGGTGGCCTTTTTATTGCTATGGGTTGGGGCTGTGAACCAAGTTGGCTTTCATCACAGTGGATCCGCCTTAAACCTTTCCGGGTTGTCTGTCACAATTGGTATATATGGTTTCGGCTACGCTGGTCATTCAGTGTTCCCAAATATCTACACATCAATGAGAAAACCATCTCAATTTCCAGCAGTTCTACTAACCTGTGGTGCCTTTTGCTTCTTCATGTATACTGGGGTAGCAATATGTGGCTATCTAATGTTTGGAGACTCGGTCAGGTCTCAGTTCACTCTAAACATGCCCGAAGAATTTGTTGCTTCAAAAATTGCAGTCTGGACAACAATTGTGAACCCGATGACAAAGTATGCCCTCACAATAACGCCCGTTGCTCTGAGTTTAGAGGAACTCTTTCCTGTAGCTTGGATGGGATCTCATTTGGCTTCAATCCTTACAAGAACAGTTCTAGTTCTATCAACTTTGGTCGTAGCGTTGAGCTTTCCATTCTTTGGTGCGGTGATGGCATTGATGGGATCCTTACTTGCAATGTTAGTGGCTATTATCTTCCCAGGGGTGTGTTATCTCATCCTACTTAAAGGTAGATTAACTAAGTCGCAGATTACAACTTGCGTTTTCGTTATAATGCTGGGGGTGTTGTGCTCTATTGTTGGCACATATTCATCAATTACAAAAATAGCTGAACAAATGGACTAA

Protein Analysis

542

Amino Acids

58.53

Weight (kDa)

5.01

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 153 - 534 6.9e-67 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011681)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09330 AT3G09340 AT5G02170 AT5G02170 AT5G02180 AT5G02180
fragaria_vesca FvH4_6g13370 FvH4_6g13370
malus_domestica MD12G1144700.v1.1
prunus_persica Prupe.6G258200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0466251
rosa_laevigata RLG00000024593
rosa_multiflora Rmu_sc0000350.1_g000014 Rmu_sc0022084.1_g000006
rosa_roxburghii Rroxscaffold_6G00414690
rosa_rugosa Rorug03G0080600
rosa_samantha Rh3BG148000 Rh3DG148700
rosa_wichuraiana Rw3G011960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1556
AccB1I GGYRCC 1 cut(s) 1127
AccB7I CCANNNNNTGG 3 cut(s) 656, 867, 1424
AccI GTMKAC 1 cut(s) 1148
AccII CGCG 1 cut(s) 45
AciI CCGC 6 cut(s) 45, 411, 440, 767, 993, 1430
AclWI GGATC 7 cut(s) 36, 48, 984, 997, 1354, 1443, 1456
AcoI YGGCCR 2 cut(s) 658, 682
AcsI RAATTY 4 cut(s) 418, 600, 825, 1226
AcuI CTGAAG 1 cut(s) 150
AfaI GTAC 2 cut(s) 215, 477
AfiI CCNNNNNNNGG 4 cut(s) 44, 656, 867, 1424
AgsI TTSAA 3 cut(s) 815, 1239, 1362
AjnI CCWGG 3 cut(s) 631, 660, 1483
AjuI GAANNNNNNNTTGG 2 cut(s) 960, 992
AluBI AGCT 8 cut(s) 19, 192, 279, 334, 473, 1337, 1413, 1613
AluI AGCT 8 cut(s) 19, 192, 279, 334, 473, 1337, 1413, 1613
Alw21I GWGCWC 2 cut(s) 166, 1577
Alw26I GTCTC 2 cut(s) 1179, 1203
Alw44I GTGCAC 1 cut(s) 162
AlwI GGATC 7 cut(s) 36, 48, 984, 997, 1354, 1443, 1456
AoxI GGCC 5 cut(s) 182, 266, 658, 682, 933
ApaI GGGCCC 1 cut(s) 270
ApaLI GTGCAC 1 cut(s) 162
ApeKI GCWGC 2 cut(s) 386, 665
ApoI RAATTY 4 cut(s) 418, 600, 825, 1226
AseI ATTAAT 1 cut(s) 608
Asp700I GAANNNNTTC 1 cut(s) 1326
AspS9I GGNCC 3 cut(s) 35, 266, 267
AsuC2I CCSGG 1 cut(s) 1009
AsuHPI GGTGA 1 cut(s) 1444
AvaII GGWCC 1 cut(s) 35
AxyI CCTNAGG 1 cut(s) 144
BaeGI GKGCMC 2 cut(s) 166, 270
BalI TGGCCA 1 cut(s) 660
BamHI GGATCC 2 cut(s) 989, 1448
BanI GGYRCC 1 cut(s) 1127
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 2 cut(s) 166, 1577
BbvI GCAGC 2 cut(s) 398, 652
BccI CCATC 9 cut(s) 212, 499, 745, 816, 842, 1102, 1336, 1429, 1438
BceAI ACGGC 1 cut(s) 669
BciT130I CCWGG 3 cut(s) 633, 662, 1485
BcnI CCSGG 1 cut(s) 1009
BcoDI GTCTC 2 cut(s) 1179, 1203
BfaI CTAG 2 cut(s) 713, 1382
BfmI CTRYAG 1 cut(s) 1332
BisI GCNGC 3 cut(s) 387, 666, 768
BlsI GCNGC 3 cut(s) 388, 667, 769
Bme1390I CCNGG 4 cut(s) 633, 662, 1009, 1485
Bme18I GGWCC 1 cut(s) 35
BmgT120I GGNCC 3 cut(s) 35, 266, 267
BmiI GGNNCC 5 cut(s) 268, 872, 991, 1129, 1450
BmrFI CCNGG 4 cut(s) 633, 662, 1009, 1485
BmrI ACTGGG 2 cut(s) 264, 1161
BmsI GCATC 1 cut(s) 398
BmuI ACTGGG 2 cut(s) 264, 1161
BpiI GAAGAC 1 cut(s) 279
BpuMI CCSGG 1 cut(s) 1009
Bsa29I ATCGAT 1 cut(s) 207
BsaBI GATNNNNATC 1 cut(s) 27
BsaI GGTCTC 1 cut(s) 1203
BsaJI CCNNGG 2 cut(s) 1483, 1484
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 1176, 1206
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 656, 867, 1424
Bse1I ACTGG 5 cut(s) 200, 270, 601, 685, 1156
Bse21I CCTNAGG 1 cut(s) 144
Bse3DI GCAATG 2 cut(s) 150, 1467
Bse8I GATNNNNATC 1 cut(s) 27
BseBI CCWGG 3 cut(s) 633, 662, 1485
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 1483, 1484
BseGI GGATG 4 cut(s) 28, 510, 1347, 1500
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 4 cut(s) 44, 656, 867, 1424
BseMI GCAATG 2 cut(s) 150, 1467
BseMII CTCAG 5 cut(s) 135, 349, 915, 1214, 1301
BseNI ACTGG 5 cut(s) 200, 270, 601, 685, 1156
BseRI GAGGAG 2 cut(s) 222, 924
BseSI GKGCMC 2 cut(s) 166, 270
BseXI GCAGC 2 cut(s) 398, 652
BseYI CCCAGC 1 cut(s) 1561
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 5 cut(s) 184, 268, 660, 684, 935
BshNI GGYRCC 1 cut(s) 1127
BshVI ATCGAT 1 cut(s) 207
BsiHKAI GWGCWC 2 cut(s) 166, 1577
BsiSI CCGG 2 cut(s) 38, 1008
BslFI GGGAC 2 cut(s) 62, 522
BslI CCNNNNNNNGG 4 cut(s) 44, 656, 867, 1424
BsmAI GTCTC 2 cut(s) 1179, 1203
BsmFI GGGAC 2 cut(s) 62, 522
BsnI GGCC 5 cut(s) 184, 268, 660, 684, 935
Bso31I GGTCTC 1 cut(s) 1203
Bsp120I GGGCCC 1 cut(s) 266
Bsp1286I GDGCHC 3 cut(s) 166, 270, 1577
Bsp1407I TGTACA 1 cut(s) 213
Bsp143I GATC 5 cut(s) 28, 40, 989, 1346, 1448
BspACI CCGC 6 cut(s) 45, 411, 440, 767, 993, 1430
BspANI GGCC 5 cut(s) 184, 268, 660, 684, 935
BspCNI CTCAG 5 cut(s) 136, 348, 914, 1213, 1302
BspDI ATCGAT 1 cut(s) 207
BspFNI CGCG 1 cut(s) 45
BspHI TCATGA 2 cut(s) 225, 820
BspLI GGNNCC 5 cut(s) 268, 872, 991, 1129, 1450
BspPI GGATC 7 cut(s) 36, 48, 984, 997, 1354, 1443, 1456
BspT107I GGYRCC 1 cut(s) 1127
BspTNI GGTCTC 1 cut(s) 1203
BsrDI GCAATG 2 cut(s) 150, 1467
BsrGI TGTACA 1 cut(s) 213
BsrI ACTGG 5 cut(s) 200, 270, 601, 685, 1156
BssECI CCNNGG 2 cut(s) 1483, 1484
BssMI GATC 5 cut(s) 28, 40, 989, 1346, 1448
BssNAI GTATAC 1 cut(s) 1149
Bst1107I GTATAC 1 cut(s) 1149
Bst2UI CCWGG 3 cut(s) 633, 662, 1485
Bst4CI ACNGT 3 cut(s) 865, 986, 1378
Bst6I CTCTTC 1 cut(s) 69
BstAUI TGTACA 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 8 cut(s) 144, 335, 763, 881, 901, 1200, 1310, 1524
BstF5I GGATG 4 cut(s) 28, 510, 1347, 1500
BstFNI CGCG 1 cut(s) 45
BstKTI GATC 5 cut(s) 31, 43, 992, 1349, 1451
BstMAI GTCTC 2 cut(s) 1179, 1203
BstMBI GATC 5 cut(s) 28, 40, 989, 1346, 1448
BstMWI GCNNNNNNNGC 5 cut(s) 161, 359, 681, 839, 1436
BstNI CCWGG 3 cut(s) 633, 662, 1485
BstNSI RCATGY 1 cut(s) 1219
BstSCI CCNGG 4 cut(s) 631, 660, 1007, 1483
BstSFI CTRYAG 1 cut(s) 1332
BstSLI GKGCMC 2 cut(s) 166, 270
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 2 cut(s) 398, 652
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 4 cut(s) 28, 989, 1346, 1448
BstYI RGATCY 4 cut(s) 28, 989, 1346, 1448
BstZ17I GTATAC 1 cut(s) 1149
Bsu15I ATCGAT 1 cut(s) 207
Bsu36I CCTNAGG 1 cut(s) 144
BsuRI GGCC 5 cut(s) 184, 268, 660, 684, 935
BsuTUI ATCGAT 1 cut(s) 207
BtgZI GCGATG 1 cut(s) 628
BtsCI GGATG 4 cut(s) 28, 510, 1347, 1500
BtsIMutI CAGTG 3 cut(s) 861, 991, 1065
Cac8I GCNNGC 1 cut(s) 384
CciI TCATGA 2 cut(s) 225, 820
Cfr13I GGNCC 3 cut(s) 35, 266, 267
ClaI ATCGAT 1 cut(s) 207
CseI GACGC 1 cut(s) 909
Csp6I GTAC 2 cut(s) 214, 476
CspCI CAANNNNNGTGG 2 cut(s) 1449, 1484
CviAII CATG 5 cut(s) 180, 226, 821, 1144, 1216
CviQI GTAC 2 cut(s) 214, 476
DdeI CTNAG 8 cut(s) 144, 335, 763, 881, 901, 1200, 1310, 1524
DpnI GATC 5 cut(s) 30, 42, 991, 1348, 1450
DpnII GATC 5 cut(s) 28, 40, 989, 1346, 1448
EaeI YGGCCR 2 cut(s) 658, 682
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
EciI GGCGGA 1 cut(s) 982
Eco24I GRGCYC 1 cut(s) 270
Eco31I GGTCTC 1 cut(s) 1203
Eco47I GGWCC 1 cut(s) 35
Eco57I CTGAAG 1 cut(s) 150
Eco81I CCTNAGG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 266
EcoRII CCWGG 3 cut(s) 631, 660, 1483
EcoT22I ATGCAT 1 cut(s) 181
EcoT38I GRGCYC 1 cut(s) 270
FaeI CATG 5 cut(s) 183, 229, 824, 1147, 1219
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FaqI GGGAC 2 cut(s) 62, 522
FatI CATG 5 cut(s) 179, 225, 820, 1143, 1215
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 1 cut(s) 526
FblI GTMKAC 1 cut(s) 1148
Fnu4HI GCNGC 3 cut(s) 387, 666, 768
FokI GGATG 4 cut(s) 35, 517, 1354, 1487
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 3 cut(s) 387, 666, 768
FspBI CTAG 2 cut(s) 713, 1382
GluI GCNGC 3 cut(s) 387, 666, 768
GsaI CCCAGC 1 cut(s) 1565
HaeIII GGCC 5 cut(s) 184, 268, 660, 684, 935
HapII CCGG 2 cut(s) 38, 1008
HgaI GACGC 1 cut(s) 909
Hin1II CATG 5 cut(s) 183, 229, 824, 1147, 1219
HinfI GANTC 7 cut(s) 125, 229, 486, 636, 804, 925, 1187
HpaII CCGG 2 cut(s) 38, 1008
HphI GGTGA 1 cut(s) 1444
Hpy166II GTNNAC 6 cut(s) 164, 216, 964, 1149, 1206, 1267
Hpy188I TCNGA 5 cut(s) 112, 130, 358, 641, 1311
Hpy188III TCNNGA 5 cut(s) 226, 650, 713, 821, 1252
Hpy8I GTNNAC 6 cut(s) 164, 216, 964, 1149, 1206, 1267
Hpy99I CGWCG 1 cut(s) 925
HpyAV CCTTC 6 cut(s) 117, 246, 251, 255, 371, 533
HpyCH4III ACNGT 3 cut(s) 865, 986, 1378
HpyCH4V TGCA 8 cut(s) 164, 179, 386, 530, 569, 800, 1247, 1460
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 359, 681, 839, 1436
HpyF3I CTNAG 8 cut(s) 144, 335, 763, 881, 901, 1200, 1310, 1524
Hsp92II CATG 5 cut(s) 183, 229, 824, 1147, 1219
Kzo9I GATC 5 cut(s) 28, 40, 989, 1346, 1448
LmnI GCTCC 2 cut(s) 284, 876
Lsp1109I GCAGC 2 cut(s) 398, 652
LweI GCATC 1 cut(s) 398
MaeI CTAG 2 cut(s) 713, 1382
MaeIII GTNAC 1 cut(s) 1018
MalI GATC 5 cut(s) 30, 42, 991, 1348, 1450
MboI GATC 5 cut(s) 28, 40, 989, 1346, 1448
MboII GAAGA 8 cut(s) 86, 279, 318, 803, 848, 1132, 1235, 1471
MfeI CAATTG 2 cut(s) 1023, 1260
MflI RGATCY 4 cut(s) 28, 989, 1346, 1448
MhlI GDGCHC 3 cut(s) 166, 270, 1577
MlsI TGGCCA 1 cut(s) 660
MluNI TGGCCA 1 cut(s) 660
MlyI GAGTC 3 cut(s) 223, 630, 1181
MmeI TCCRAC 1 cut(s) 886
Mox20I TGGCCA 1 cut(s) 660
Mph1103I ATGCAT 1 cut(s) 181
MroXI GAANNNNTTC 1 cut(s) 1326
MscI TGGCCA 1 cut(s) 660
MseI TTAA 7 cut(s) 62, 297, 608, 689, 998, 1509, 1520
MslI CAYNNNNRTG 3 cut(s) 209, 984, 1082
Msp20I TGGCCA 1 cut(s) 660
MspI CCGG 2 cut(s) 38, 1008
MspR9I CCNGG 4 cut(s) 633, 662, 1009, 1485
MunI CAATTG 2 cut(s) 1023, 1260
MvaI CCWGG 3 cut(s) 633, 662, 1485
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 5 cut(s) 161, 359, 681, 839, 1436
NciI CCSGG 1 cut(s) 1009
NdeI CATATG 1 cut(s) 526
NdeII GATC 5 cut(s) 28, 40, 989, 1346, 1448
NlaIII CATG 5 cut(s) 183, 229, 824, 1147, 1219
NlaIV GGNNCC 5 cut(s) 268, 872, 991, 1129, 1450
NmuCI GTSAC 1 cut(s) 1018
NsiI ATGCAT 1 cut(s) 181
NspI RCATGY 1 cut(s) 1219
PagI TCATGA 2 cut(s) 225, 820
PasI CCCWGGG 1 cut(s) 1484
PdmI GAANNNNTTC 1 cut(s) 1326
PfeI GAWTC 4 cut(s) 125, 486, 804, 925
PflFI GACNNNGTC 1 cut(s) 1190
PflMI CCANNNNNTGG 3 cut(s) 656, 867, 1424
PfoI TCCNGGA 1 cut(s) 631
PkrI GCNGC 3 cut(s) 388, 667, 769
PleI GAGTC 3 cut(s) 223, 630, 1181
PpsI GAGTC 3 cut(s) 223, 630, 1181
PshBI ATTAAT 1 cut(s) 608
PsiI TTATAA 1 cut(s) 1556
Psp6I CCWGG 3 cut(s) 631, 660, 1483
PspFI CCCAGC 1 cut(s) 1561
PspGI CCWGG 3 cut(s) 631, 660, 1483
PspN4I GGNNCC 5 cut(s) 268, 872, 991, 1129, 1450
PspOMI GGGCCC 1 cut(s) 266
PspPI GGNCC 3 cut(s) 35, 266, 267
PsuI RGATCY 4 cut(s) 28, 989, 1346, 1448
PsyI GACNNNGTC 1 cut(s) 1190
RsaI GTAC 2 cut(s) 215, 477
RsaNI GTAC 2 cut(s) 214, 476
RseI CAYNNNNRTG 3 cut(s) 209, 984, 1082
SaqAI TTAA 7 cut(s) 62, 297, 608, 689, 998, 1509, 1520
SatI GCNGC 3 cut(s) 387, 666, 768
Sau3AI GATC 5 cut(s) 28, 40, 989, 1346, 1448
Sau96I GGNCC 3 cut(s) 35, 266, 267
SchI GAGTC 3 cut(s) 223, 630, 1181
ScrFI CCNGG 4 cut(s) 633, 662, 1009, 1485
SduI GDGCHC 3 cut(s) 166, 270, 1577
SfaNI GCATC 1 cut(s) 398
SfcI CTRYAG 1 cut(s) 1332
SinI GGWCC 1 cut(s) 35
SmiMI CAYNNNNRTG 3 cut(s) 209, 984, 1082
SsiI CCGC 6 cut(s) 45, 411, 440, 767, 993, 1430
SspI AATATT 1 cut(s) 774
SspMI CTAG 2 cut(s) 713, 1382
StyD4I CCNGG 4 cut(s) 631, 660, 1007, 1483
TaaI ACNGT 3 cut(s) 865, 986, 1378
TaqI TCGA 3 cut(s) 207, 677, 923
TaqII GACCGA 1 cut(s) 1180
TatI WGTACW 2 cut(s) 213, 475
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 4 cut(s) 125, 486, 804, 925
Tru1I TTAA 7 cut(s) 62, 297, 608, 689, 998, 1509, 1520
Tru9I TTAA 7 cut(s) 62, 297, 608, 689, 998, 1509, 1520
TscAI CASTG 3 cut(s) 868, 991, 1065
TseFI GTSAC 1 cut(s) 1018
TseI GCWGC 2 cut(s) 386, 665
Tsp45I GTSAC 1 cut(s) 1018
TspDTI ATGAA 6 cut(s) 87, 450, 837, 968, 1132, 1584
TspRI CASTG 3 cut(s) 868, 991, 1065
Tth111I GACNNNGTC 1 cut(s) 1190
Van91I CCANNNNNTGG 3 cut(s) 656, 867, 1424
VneI GTGCAC 1 cut(s) 162
VpaK11BI GGWCC 1 cut(s) 35
VspI ATTAAT 1 cut(s) 608
XapI RAATTY 4 cut(s) 418, 600, 825, 1226
XbaI TCTAGA 1 cut(s) 712
XceI RCATGY 1 cut(s) 1219
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XmiI GTMKAC 1 cut(s) 1148
XmnI GAANNNNTTC 1 cut(s) 1326
XspI CTAG 2 cut(s) 713, 1382
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.