Rh3DG148700

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0011681
Physical Location & Seq
Forward (+)
12749209 .. 12752523
3315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG148700.1

Sequence Viewer

Length: 1482 bp
ATGAGCAGTTGGACGAAGCTGGATGAGGATCTTGGACCGGAGCGCGGGGACGATGATTTTTTAACAGATGATGAAGAGAACCAAGCACACAGAAACAACTATGAGCCTTCCGAAGCCGAAACTGAATCTGAAGTAGGCAGACCTGAGGGCATTGCCATTAGTGCACCTAACAATAATGCATGGCCTCAGAGCTACTGGCAGTCCATCGATATGTACACCAATGTCATGACTCCTCCTTCCCTTCCTTCCCTTACTTTTATGCGATCAAGAGGGCCCAGTTTCAGCTCCTTGTCTTCGTTTGTTAAGGGGCAACAAGCACCTGATGATAATTCTTCACAGCTCAGTAGCAAATTACTTGCTTCCGAAGCAAGTTTAGAAAAAGAAGGGCTTGCAGCATCATCTCCCCCAACTCTCTCCGCAAAAAATTCAGTCTGTGAATTGCCACCGCCAAAGGAACATAGTTGTTCATTTTCACAAGCTGTACTCAATGGAATCAATGTCTTGTATATTGGCCAGGCTGCTTTTGGCTCTACTGGCCGTTTAATTCTATCTATAATTTTGTATCTAGAACTATATGGTGCTTGTGTGGAGTTTTTGACGATGATGGGCGATAACTTAGCGGCAATATTCCCAAACACCCACATAGCATTTGCAGGAATCCATCTTCAAAGTCATGAAATTTTTGCCATCACAGCGACTCTTGTAGTTCTTCCCACTGTATGGCTTCAAAATCTTAGTTTGCTCTCTTACCTCTCAGTTGGAGGAGTAGGTGCATCGATTCTGGTGGCCTTTTTATTGCTGTGGGTTGGGGCTGTGAACCAAGTTGGCTTTCATCACAGTGGATCCGCCTTAAACCTTTCCGGTTTGTCTGTCACAATTGGTATATATGGTTTCGGCTACGCTGGTCATTCAGTGTTCCCAAATATCTACACATCTATGAGAAAACCATCTCAATTTCCAGCAGTTCTACTAACCTGTGGTGCCTTTTGCTTCTTCATGTATACTGGGGTAGCAATATGTGGCTATCTAATGTTTGGAGACTCGGTGAGGTCTCAGTTCACTCTAAACATGCCCGAAGAATTTGTTGCTTCAAAAATTGCAGTCTGGACAACAATTGTGAACCCGATGACAAAGTATGCCCTCACAATAACGCCCGTTGCTCTGAGTTTAGAAGAACTCTTTCCTGTAGCTTGGATGGGATCTCATTTGGCTTCAATCCTTACAAGAACAGTTCTAGTTCTATCAACTTTGGTCGTAGCGTTGAGCTTTCCATTCTTTGGTGCGGTGATGGCATTGATGGGATCCTTACTTGCAATGTTAGTGGCTATTATCTTCCCAGGGGTCTGTTATCTCATCCTACTTAAAGGTAGATTAACTAAGTCGCAGATTACAACTTGCGTTTTCGTTATAATGCTGGGGGTGTTGTGCTCTATTATTGGCACATATTCATCAATTACAAAAATAGCTGAACAAATGGACTAA

Protein Analysis

493

Amino Acids

53.19

Weight (kDa)

5.06

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 170 - 485 2e-46 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011681)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09330 AT3G09340 AT5G02170 AT5G02170 AT5G02180 AT5G02180
fragaria_vesca FvH4_6g13370 FvH4_6g13370
malus_domestica MD12G1144700.v1.1
prunus_persica Prupe.6G258200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0466251
rosa_laevigata RLG00000024593
rosa_multiflora Rmu_sc0000350.1_g000014 Rmu_sc0022084.1_g000006
rosa_roxburghii Rroxscaffold_6G00414690
rosa_rugosa Rorug03G0080600
rosa_samantha Rh3BG148000 Rh3DG148700
rosa_wichuraiana Rw3G011960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1409
AccB1I GGYRCC 1 cut(s) 980
AccB7I CCANNNNNTGG 2 cut(s) 720, 1277
AccI GTMKAC 1 cut(s) 1001
AccII CGCG 1 cut(s) 45
AciI CCGC 6 cut(s) 45, 417, 446, 620, 846, 1283
AclWI GGATC 6 cut(s) 36, 837, 850, 1207, 1296, 1309
AcoI YGGCCR 2 cut(s) 511, 535
AcsI RAATTY 3 cut(s) 424, 678, 1079
AcuI CTGAAG 1 cut(s) 150
AfaI GTAC 2 cut(s) 215, 483
AfiI CCNNNNNNNGG 3 cut(s) 44, 720, 1277
AgsI TTSAA 4 cut(s) 668, 728, 1092, 1215
AjnI CCWGG 2 cut(s) 513, 1336
AjuI GAANNNNNNNTTGG 2 cut(s) 813, 845
AluBI AGCT 8 cut(s) 19, 192, 285, 340, 479, 1190, 1266, 1466
AluI AGCT 8 cut(s) 19, 192, 285, 340, 479, 1190, 1266, 1466
Alw21I GWGCWC 2 cut(s) 166, 1430
Alw26I GTCTC 2 cut(s) 1032, 1056
Alw44I GTGCAC 1 cut(s) 162
AlwI GGATC 6 cut(s) 36, 837, 850, 1207, 1296, 1309
AoxI GGCC 5 cut(s) 182, 272, 511, 535, 786
ApaI GGGCCC 1 cut(s) 276
ApaLI GTGCAC 1 cut(s) 162
ApeKI GCWGC 2 cut(s) 392, 518
ApoI RAATTY 3 cut(s) 424, 678, 1079
Asp700I GAANNNNTTC 1 cut(s) 1179
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 3 cut(s) 35, 272, 273
AsuHPI GGTGA 2 cut(s) 1057, 1297
AvaII GGWCC 1 cut(s) 35
AxyI CCTNAGG 1 cut(s) 144
BaeGI GKGCMC 2 cut(s) 166, 276
BalI TGGCCA 1 cut(s) 513
BamHI GGATCC 2 cut(s) 842, 1301
BanI GGYRCC 1 cut(s) 980
BanII GRGCYC 1 cut(s) 276
BbsI GAAGAC 1 cut(s) 285
Bbv12I GWGCWC 2 cut(s) 166, 1430
BbvI GCAGC 2 cut(s) 404, 505
BccI CCATC 8 cut(s) 212, 598, 669, 695, 955, 1189, 1282, 1291
BceAI ACGGC 1 cut(s) 522
BciT130I CCWGG 2 cut(s) 515, 1338
BcoDI GTCTC 2 cut(s) 1032, 1056
BfaI CTAG 2 cut(s) 566, 1235
BfmI CTRYAG 1 cut(s) 1185
BisI GCNGC 3 cut(s) 393, 519, 621
BlsI GCNGC 3 cut(s) 394, 520, 622
Bme1390I CCNGG 2 cut(s) 515, 1338
Bme18I GGWCC 1 cut(s) 35
BmgT120I GGNCC 3 cut(s) 35, 272, 273
BmiI GGNNCC 4 cut(s) 274, 844, 982, 1303
BmrFI CCNGG 2 cut(s) 515, 1338
BmrI ACTGGG 2 cut(s) 270, 1014
BmsI GCATC 2 cut(s) 404, 782
BmuI ACTGGG 2 cut(s) 270, 1014
BpiI GAAGAC 1 cut(s) 285
Bsa29I ATCGAT 2 cut(s) 207, 776
BsaBI GATNNNNATC 1 cut(s) 27
BsaI GGTCTC 1 cut(s) 1056
BsaJI CCNNGG 2 cut(s) 1336, 1337
BsaWI WCCGGW 2 cut(s) 37, 860
BsaXI ACNNNNNCTCC 2 cut(s) 1029, 1059
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 720, 1277
Bse1I ACTGG 4 cut(s) 200, 276, 538, 1009
Bse21I CCTNAGG 1 cut(s) 144
Bse3DI GCAATG 2 cut(s) 150, 1320
Bse8I GATNNNNATC 1 cut(s) 27
BseBI CCWGG 2 cut(s) 515, 1338
BseCI ATCGAT 2 cut(s) 207, 776
BseDI CCNNGG 2 cut(s) 1336, 1337
BseGI GGATG 3 cut(s) 28, 1200, 1353
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 3 cut(s) 44, 720, 1277
BseMI GCAATG 2 cut(s) 150, 1320
BseMII CTCAG 6 cut(s) 135, 200, 355, 768, 1067, 1154
BseNI ACTGG 4 cut(s) 200, 276, 538, 1009
BseRI GAGGAG 2 cut(s) 222, 777
BseSI GKGCMC 2 cut(s) 166, 276
BseXI GCAGC 2 cut(s) 404, 505
BseYI CCCAGC 1 cut(s) 1414
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 5 cut(s) 184, 274, 513, 537, 788
BshNI GGYRCC 1 cut(s) 980
BshVI ATCGAT 2 cut(s) 207, 776
BsiHKAI GWGCWC 2 cut(s) 166, 1430
BsiSI CCGG 2 cut(s) 38, 861
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 3 cut(s) 44, 720, 1277
BsmAI GTCTC 2 cut(s) 1032, 1056
BsmFI GGGAC 1 cut(s) 62
BsnI GGCC 5 cut(s) 184, 274, 513, 537, 788
Bso31I GGTCTC 1 cut(s) 1056
Bsp120I GGGCCC 1 cut(s) 272
Bsp1286I GDGCHC 3 cut(s) 166, 276, 1430
Bsp1407I TGTACA 1 cut(s) 213
Bsp143I GATC 5 cut(s) 28, 263, 842, 1199, 1301
BspACI CCGC 6 cut(s) 45, 417, 446, 620, 846, 1283
BspANI GGCC 5 cut(s) 184, 274, 513, 537, 788
BspCNI CTCAG 6 cut(s) 136, 199, 354, 767, 1066, 1155
BspDI ATCGAT 2 cut(s) 207, 776
BspFNI CGCG 1 cut(s) 45
BspHI TCATGA 2 cut(s) 225, 673
BspLI GGNNCC 4 cut(s) 274, 844, 982, 1303
BspPI GGATC 6 cut(s) 36, 837, 850, 1207, 1296, 1309
BspT107I GGYRCC 1 cut(s) 980
BspTNI GGTCTC 1 cut(s) 1056
BsrDI GCAATG 2 cut(s) 150, 1320
BsrGI TGTACA 1 cut(s) 213
BsrI ACTGG 4 cut(s) 200, 276, 538, 1009
BssECI CCNNGG 2 cut(s) 1336, 1337
BssMI GATC 5 cut(s) 28, 263, 842, 1199, 1301
BssNAI GTATAC 1 cut(s) 1002
Bst1107I GTATAC 1 cut(s) 1002
Bst2UI CCWGG 2 cut(s) 515, 1338
Bst4CI ACNGT 3 cut(s) 718, 839, 1231
Bst6I CTCTTC 1 cut(s) 69
BstAUI TGTACA 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 9 cut(s) 144, 186, 341, 616, 734, 754, 1053, 1163, 1377
BstF5I GGATG 3 cut(s) 28, 1200, 1353
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 5 cut(s) 31, 266, 845, 1202, 1304
BstMAI GTCTC 2 cut(s) 1032, 1056
BstMBI GATC 5 cut(s) 28, 263, 842, 1199, 1301
BstMWI GCNNNNNNNGC 5 cut(s) 161, 365, 534, 692, 1289
BstNI CCWGG 2 cut(s) 515, 1338
BstNSI RCATGY 1 cut(s) 1072
BstSCI CCNGG 2 cut(s) 513, 1336
BstSFI CTRYAG 1 cut(s) 1185
BstSLI GKGCMC 2 cut(s) 166, 276
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 2 cut(s) 404, 505
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 4 cut(s) 28, 842, 1199, 1301
BstYI RGATCY 4 cut(s) 28, 842, 1199, 1301
BstZ17I GTATAC 1 cut(s) 1002
Bsu15I ATCGAT 2 cut(s) 207, 776
Bsu36I CCTNAGG 1 cut(s) 144
BsuRI GGCC 5 cut(s) 184, 274, 513, 537, 788
BsuTUI ATCGAT 2 cut(s) 207, 776
BtsCI GGATG 3 cut(s) 28, 1200, 1353
BtsIMutI CAGTG 3 cut(s) 714, 844, 918
Cac8I GCNNGC 1 cut(s) 390
CciI TCATGA 2 cut(s) 225, 673
CfoI GCGC 1 cut(s) 45
Cfr13I GGNCC 3 cut(s) 35, 272, 273
ClaI ATCGAT 2 cut(s) 207, 776
Csp6I GTAC 2 cut(s) 214, 482
CspCI CAANNNNNGTGG 2 cut(s) 1302, 1337
CviAII CATG 5 cut(s) 180, 226, 674, 997, 1069
CviQI GTAC 2 cut(s) 214, 482
DdeI CTNAG 9 cut(s) 144, 186, 341, 616, 734, 754, 1053, 1163, 1377
DpnI GATC 5 cut(s) 30, 265, 844, 1201, 1303
DpnII GATC 5 cut(s) 28, 263, 842, 1199, 1301
EaeI YGGCCR 2 cut(s) 511, 535
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
EciI GGCGGA 1 cut(s) 835
Eco24I GRGCYC 1 cut(s) 276
Eco31I GGTCTC 1 cut(s) 1056
Eco47I GGWCC 1 cut(s) 35
Eco57I CTGAAG 1 cut(s) 150
Eco81I CCTNAGG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 513, 1336
EcoT22I ATGCAT 1 cut(s) 181
EcoT38I GRGCYC 1 cut(s) 276
FaeI CATG 5 cut(s) 183, 229, 677, 1000, 1072
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FaqI GGGAC 1 cut(s) 62
FatI CATG 5 cut(s) 179, 225, 673, 996, 1068
FauI CCCGC 1 cut(s) 38
FblI GTMKAC 1 cut(s) 1001
Fnu4HI GCNGC 3 cut(s) 393, 519, 621
FokI GGATG 3 cut(s) 35, 1207, 1340
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 3 cut(s) 393, 519, 621
FspBI CTAG 2 cut(s) 566, 1235
GlaI GCGC 1 cut(s) 44
GluI GCNGC 3 cut(s) 393, 519, 621
GsaI CCCAGC 1 cut(s) 1418
HaeIII GGCC 5 cut(s) 184, 274, 513, 537, 788
HapII CCGG 2 cut(s) 38, 861
HhaI GCGC 1 cut(s) 45
Hin1II CATG 5 cut(s) 183, 229, 677, 1000, 1072
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HinfI GANTC 7 cut(s) 125, 229, 492, 657, 697, 778, 1040
HpaII CCGG 2 cut(s) 38, 861
HphI GGTGA 2 cut(s) 1057, 1297
Hpy166II GTNNAC 6 cut(s) 164, 216, 817, 1002, 1059, 1120
Hpy188I TCNGA 5 cut(s) 112, 130, 189, 364, 1164
Hpy188III TCNNGA 5 cut(s) 226, 267, 566, 674, 1105
Hpy8I GTNNAC 6 cut(s) 164, 216, 817, 1002, 1059, 1120
HpyAV CCTTC 5 cut(s) 117, 246, 251, 255, 377
HpyCH4III ACNGT 3 cut(s) 718, 839, 1231
HpyCH4V TGCA 7 cut(s) 164, 179, 392, 653, 773, 1100, 1313
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 365, 534, 692, 1289
HpyF3I CTNAG 9 cut(s) 144, 186, 341, 616, 734, 754, 1053, 1163, 1377
Hsp92II CATG 5 cut(s) 183, 229, 677, 1000, 1072
HspAI GCGC 1 cut(s) 43
Kzo9I GATC 5 cut(s) 28, 263, 842, 1199, 1301
LmnI GCTCC 2 cut(s) 40, 290
Lsp1109I GCAGC 2 cut(s) 404, 505
LweI GCATC 2 cut(s) 404, 782
MaeI CTAG 2 cut(s) 566, 1235
MaeIII GTNAC 1 cut(s) 871
MalI GATC 5 cut(s) 30, 265, 844, 1201, 1303
MboI GATC 5 cut(s) 28, 263, 842, 1199, 1301
MboII GAAGA 9 cut(s) 86, 285, 324, 656, 701, 985, 1088, 1184, 1324
MfeI CAATTG 2 cut(s) 876, 1113
MflI RGATCY 4 cut(s) 28, 842, 1199, 1301
MhlI GDGCHC 3 cut(s) 166, 276, 1430
MlsI TGGCCA 1 cut(s) 513
MluNI TGGCCA 1 cut(s) 513
MlyI GAGTC 3 cut(s) 223, 691, 1034
MmeI TCCRAC 1 cut(s) 739
MnlI CCTC 9 cut(s) 19, 139, 195, 243, 263, 755, 761, 1041, 1151
Mox20I TGGCCA 1 cut(s) 513
Mph1103I ATGCAT 1 cut(s) 181
MroXI GAANNNNTTC 1 cut(s) 1179
MscI TGGCCA 1 cut(s) 513
MseI TTAA 6 cut(s) 62, 303, 542, 851, 1362, 1373
MslI CAYNNNNRTG 3 cut(s) 209, 837, 935
Msp20I TGGCCA 1 cut(s) 513
MspI CCGG 2 cut(s) 38, 861
MspR9I CCNGG 2 cut(s) 515, 1338
MunI CAATTG 2 cut(s) 876, 1113
MvaI CCWGG 2 cut(s) 515, 1338
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 5 cut(s) 161, 365, 534, 692, 1289
NdeII GATC 5 cut(s) 28, 263, 842, 1199, 1301
NlaIII CATG 5 cut(s) 183, 229, 677, 1000, 1072
NlaIV GGNNCC 4 cut(s) 274, 844, 982, 1303
NmuCI GTSAC 1 cut(s) 871
NsiI ATGCAT 1 cut(s) 181
NspI RCATGY 1 cut(s) 1072
PagI TCATGA 2 cut(s) 225, 673
PasI CCCWGGG 1 cut(s) 1337
PdmI GAANNNNTTC 1 cut(s) 1179
PfeI GAWTC 4 cut(s) 125, 492, 657, 778
PflMI CCANNNNNTGG 2 cut(s) 720, 1277
PkrI GCNGC 3 cut(s) 394, 520, 622
PleI GAGTC 3 cut(s) 223, 691, 1034
PpsI GAGTC 3 cut(s) 223, 691, 1034
PsiI TTATAA 1 cut(s) 1409
Psp6I CCWGG 2 cut(s) 513, 1336
PspFI CCCAGC 1 cut(s) 1414
PspGI CCWGG 2 cut(s) 513, 1336
PspN4I GGNNCC 4 cut(s) 274, 844, 982, 1303
PspOMI GGGCCC 1 cut(s) 272
PspPI GGNCC 3 cut(s) 35, 272, 273
PsuI RGATCY 4 cut(s) 28, 842, 1199, 1301
RsaI GTAC 2 cut(s) 215, 483
RsaNI GTAC 2 cut(s) 214, 482
RseI CAYNNNNRTG 3 cut(s) 209, 837, 935
SaqAI TTAA 6 cut(s) 62, 303, 542, 851, 1362, 1373
SatI GCNGC 3 cut(s) 393, 519, 621
Sau3AI GATC 5 cut(s) 28, 263, 842, 1199, 1301
Sau96I GGNCC 3 cut(s) 35, 272, 273
SchI GAGTC 3 cut(s) 223, 691, 1034
ScrFI CCNGG 2 cut(s) 515, 1338
SduI GDGCHC 3 cut(s) 166, 276, 1430
SfaNI GCATC 2 cut(s) 404, 782
SfcI CTRYAG 1 cut(s) 1185
SinI GGWCC 1 cut(s) 35
SmiMI CAYNNNNRTG 3 cut(s) 209, 837, 935
SsiI CCGC 6 cut(s) 45, 417, 446, 620, 846, 1283
SspI AATATT 1 cut(s) 627
SspMI CTAG 2 cut(s) 566, 1235
StyD4I CCNGG 2 cut(s) 513, 1336
TaaI ACNGT 3 cut(s) 718, 839, 1231
TaqI TCGA 2 cut(s) 207, 776
TatI WGTACW 2 cut(s) 213, 481
TauI GCSGC 1 cut(s) 623
TfiI GAWTC 4 cut(s) 125, 492, 657, 778
Tru1I TTAA 6 cut(s) 62, 303, 542, 851, 1362, 1373
Tru9I TTAA 6 cut(s) 62, 303, 542, 851, 1362, 1373
TscAI CASTG 3 cut(s) 721, 844, 918
TseFI GTSAC 1 cut(s) 871
TseI GCWGC 2 cut(s) 392, 518
Tsp45I GTSAC 1 cut(s) 871
TspDTI ATGAA 6 cut(s) 87, 456, 690, 821, 985, 1437
TspRI CASTG 3 cut(s) 721, 844, 918
Van91I CCANNNNNTGG 2 cut(s) 720, 1277
VneI GTGCAC 1 cut(s) 162
VpaK11BI GGWCC 1 cut(s) 35
XapI RAATTY 3 cut(s) 424, 678, 1079
XbaI TCTAGA 1 cut(s) 565
XceI RCATGY 1 cut(s) 1072
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XmiI GTMKAC 1 cut(s) 1001
XmnI GAANNNNTTC 1 cut(s) 1179
XspI CTAG 2 cut(s) 566, 1235
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.