Rh3BG148000

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0011681
Physical Location & Seq
Forward (+)
12557681 .. 12562131
4451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG148000.1

Sequence Viewer

Length: 1272 bp
ATGAGCAGTTGGACGAAGCTGGATGAGGATCTTGGACCGGAGCGCGGGGACGATGATTTTTTAACAGATGATGAAGAGAACCAAGCACACAGAAACAACTATGAGCCTTCCGAAGCCGAAACTGAATCTGAAGTAGGCAGACCTGAGGGCATTGCCATTAGTGCACCTAACAATAATGCATGGCCTCAGAGCTACTGGCAGTCCATCGATATGTACACCAATGTCATGACTCCTCCTTCCCTTCCTTCCCTTACTTTTATGCGATCAAGAGGGCCCAGTTTCAGCTCCTTGTCTTCGTTTGTTAAGGGGCAACAAGCACCTGATGATAATTCTTCACAGCTCAGTAGCAAATTACTTGCTTCCGAAGCAAGTTTAGAAAAAGAAGGGCTTGCAGCATCATCTCCCCCAACTCTCTCCGCAAAAAATTCAGTCTGTGAATTGCCACCGCCAAAGGAACATAGTTGTTCATTTTCACAAGCTGTACTCAATGGAATCAATGTCTTGTGTGGGATGGGACTTATTACAACACCATATGCAATAAAAGAAGGAGGGTGGTTGAGCCTCATTTTACTTGCATTGTTTGGTGTGATATGTTGCTATACTGGAATTTTATTAATGCGATGTTTAGAAAGTTGTCCCGGACTCCGAACCTATCCAGATATTGGCCAGGCTGCTTTTGGCTCTACTGGCCGTTTAATTCTATCTATAATTTTGTATCTAGAACTATATGGTGCTTGTGTGGAGTTTTTGACGATGATGGGCGATAACTTAGCGGCAATATTCCCAAACACCCACATAGCATTTGCAGGAATCCATCTTCAAAGTCATGAAATTTTTGCCATCACAGCGACTCTTGTAGTTCTTCCCACTGTATGGCTTCAAAATCTTAGTTTGCTCTCTTACCTCTCAGTTGGAGGAGTAGGTGCATCGATTCTGGTGGCCTTTTTATTGCTGTGGGTTGGGGCTGTGAACCAAGTTGGCTTTCATCACAGTGGATCCGCCTTAAACCTTTCCGGTTTGTCTGTCACAATTGGTATATATGGTTTCGGCTACGCTGGTCATTCAGTGTTCCCAAATATCTACACATCTATGAGAAAACCATCTCAATTTCCAGCAGTTCTACTAACCTGTGGTGCCTTTTGCTTCTTCATGTATACTGGGGTAGCAATATGTGGCTATCTAATGTTTGGAGACTCGGTGAGGTCTCAGTTCACTCTAAACATGCCCGAAGAATTTGTTGCTTCAAAAATTGCAGTCTGGACAACAGTATGA

Protein Analysis

423

Amino Acids

45.64

Weight (kDa)

4.82

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 155 - 413 2e-51 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011681)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09330 AT3G09340 AT5G02170 AT5G02170 AT5G02180 AT5G02180
fragaria_vesca FvH4_6g13370 FvH4_6g13370
malus_domestica MD12G1144700.v1.1
prunus_persica Prupe.6G258200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0466251
rosa_laevigata RLG00000024593
rosa_multiflora Rmu_sc0000350.1_g000014 Rmu_sc0022084.1_g000006
rosa_roxburghii Rroxscaffold_6G00414690
rosa_rugosa Rorug03G0080600
rosa_samantha Rh3BG148000 Rh3DG148700
rosa_wichuraiana Rw3G011960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1133
AccB7I CCANNNNNTGG 2 cut(s) 662, 873
AccI GTMKAC 1 cut(s) 1154
AccII CGCG 1 cut(s) 45
AciI CCGC 5 cut(s) 45, 417, 446, 773, 999
AclWI GGATC 3 cut(s) 36, 990, 1003
AcoI YGGCCR 2 cut(s) 664, 688
AcsI RAATTY 4 cut(s) 424, 606, 831, 1232
AcuI CTGAAG 1 cut(s) 150
AfaI GTAC 2 cut(s) 215, 483
AfiI CCNNNNNNNGG 3 cut(s) 44, 662, 873
AgsI TTSAA 3 cut(s) 821, 881, 1245
AjnI CCWGG 1 cut(s) 666
AjuI GAANNNNNNNTTGG 2 cut(s) 966, 998
AluBI AGCT 5 cut(s) 19, 192, 285, 340, 479
AluI AGCT 5 cut(s) 19, 192, 285, 340, 479
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 2 cut(s) 1185, 1209
Alw44I GTGCAC 1 cut(s) 162
AlwI GGATC 3 cut(s) 36, 990, 1003
AoxI GGCC 5 cut(s) 182, 272, 664, 688, 939
ApaI GGGCCC 1 cut(s) 276
ApaLI GTGCAC 1 cut(s) 162
ApeKI GCWGC 2 cut(s) 392, 671
ApoI RAATTY 4 cut(s) 424, 606, 831, 1232
AseI ATTAAT 1 cut(s) 614
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 3 cut(s) 35, 272, 273
AsuC2I CCSGG 1 cut(s) 639
AsuHPI GGTGA 1 cut(s) 1210
AvaII GGWCC 1 cut(s) 35
AxyI CCTNAGG 1 cut(s) 144
BaeGI GKGCMC 2 cut(s) 166, 276
BalI TGGCCA 1 cut(s) 666
BamHI GGATCC 1 cut(s) 995
BanI GGYRCC 1 cut(s) 1133
BanII GRGCYC 1 cut(s) 276
BbsI GAAGAC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 404, 658
BccI CCATC 6 cut(s) 212, 505, 751, 822, 848, 1108
BceAI ACGGC 1 cut(s) 675
BciT130I CCWGG 1 cut(s) 668
BcnI CCSGG 1 cut(s) 639
BcoDI GTCTC 2 cut(s) 1185, 1209
BfaI CTAG 1 cut(s) 719
BisI GCNGC 3 cut(s) 393, 672, 774
BlsI GCNGC 3 cut(s) 394, 673, 775
Bme1390I CCNGG 2 cut(s) 639, 668
Bme18I GGWCC 1 cut(s) 35
BmgT120I GGNCC 3 cut(s) 35, 272, 273
BmiI GGNNCC 3 cut(s) 274, 997, 1135
BmrFI CCNGG 2 cut(s) 639, 668
BmrI ACTGGG 2 cut(s) 270, 1167
BmsI GCATC 2 cut(s) 404, 935
BmuI ACTGGG 2 cut(s) 270, 1167
BpiI GAAGAC 1 cut(s) 285
BpuMI CCSGG 1 cut(s) 639
Bsa29I ATCGAT 2 cut(s) 207, 929
BsaBI GATNNNNATC 1 cut(s) 27
BsaI GGTCTC 1 cut(s) 1209
BsaWI WCCGGW 2 cut(s) 37, 1013
BsaXI ACNNNNNCTCC 2 cut(s) 1182, 1212
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 662, 873
Bse1I ACTGG 5 cut(s) 200, 276, 607, 691, 1162
Bse21I CCTNAGG 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 150
Bse8I GATNNNNATC 1 cut(s) 27
BseBI CCWGG 1 cut(s) 668
BseCI ATCGAT 2 cut(s) 207, 929
BseGI GGATG 2 cut(s) 28, 516
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 3 cut(s) 44, 662, 873
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 5 cut(s) 135, 200, 355, 921, 1220
BseNI ACTGG 5 cut(s) 200, 276, 607, 691, 1162
BseRI GAGGAG 2 cut(s) 222, 930
BseSI GKGCMC 2 cut(s) 166, 276
BseXI GCAGC 2 cut(s) 404, 658
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 5 cut(s) 184, 274, 666, 690, 941
BshNI GGYRCC 1 cut(s) 1133
BshVI ATCGAT 2 cut(s) 207, 929
BsiHKAI GWGCWC 1 cut(s) 166
BsiSI CCGG 3 cut(s) 38, 639, 1014
BslFI GGGAC 3 cut(s) 62, 528, 621
BslI CCNNNNNNNGG 3 cut(s) 44, 662, 873
BsmAI GTCTC 2 cut(s) 1185, 1209
BsmFI GGGAC 3 cut(s) 62, 528, 621
BsnI GGCC 5 cut(s) 184, 274, 666, 690, 941
Bso31I GGTCTC 1 cut(s) 1209
Bsp120I GGGCCC 1 cut(s) 272
Bsp1286I GDGCHC 2 cut(s) 166, 276
Bsp1407I TGTACA 1 cut(s) 213
Bsp143I GATC 3 cut(s) 28, 263, 995
BspACI CCGC 5 cut(s) 45, 417, 446, 773, 999
BspANI GGCC 5 cut(s) 184, 274, 666, 690, 941
BspCNI CTCAG 5 cut(s) 136, 199, 354, 920, 1219
BspDI ATCGAT 2 cut(s) 207, 929
BspFNI CGCG 1 cut(s) 45
BspHI TCATGA 2 cut(s) 225, 826
BspLI GGNNCC 3 cut(s) 274, 997, 1135
BspPI GGATC 3 cut(s) 36, 990, 1003
BspT107I GGYRCC 1 cut(s) 1133
BspTNI GGTCTC 1 cut(s) 1209
BsrDI GCAATG 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 213
BsrI ACTGG 5 cut(s) 200, 276, 607, 691, 1162
BssMI GATC 3 cut(s) 28, 263, 995
BssNAI GTATAC 1 cut(s) 1155
Bst1107I GTATAC 1 cut(s) 1155
Bst2UI CCWGG 1 cut(s) 668
Bst4CI ACNGT 3 cut(s) 871, 992, 1267
Bst6I CTCTTC 1 cut(s) 69
BstAUI TGTACA 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 7 cut(s) 144, 186, 341, 769, 887, 907, 1206
BstF5I GGATG 2 cut(s) 28, 516
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 3 cut(s) 31, 266, 998
BstMAI GTCTC 2 cut(s) 1185, 1209
BstMBI GATC 3 cut(s) 28, 263, 995
BstMWI GCNNNNNNNGC 4 cut(s) 161, 365, 687, 845
BstNI CCWGG 1 cut(s) 668
BstNSI RCATGY 1 cut(s) 1225
BstSCI CCNGG 2 cut(s) 637, 666
BstSLI GKGCMC 2 cut(s) 166, 276
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 2 cut(s) 404, 658
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 2 cut(s) 28, 995
BstYI RGATCY 2 cut(s) 28, 995
BstZ17I GTATAC 1 cut(s) 1155
Bsu15I ATCGAT 2 cut(s) 207, 929
Bsu36I CCTNAGG 1 cut(s) 144
BsuRI GGCC 5 cut(s) 184, 274, 666, 690, 941
BsuTUI ATCGAT 2 cut(s) 207, 929
BtgZI GCGATG 1 cut(s) 634
BtsCI GGATG 2 cut(s) 28, 516
BtsIMutI CAGTG 3 cut(s) 867, 997, 1071
Cac8I GCNNGC 1 cut(s) 390
CciI TCATGA 2 cut(s) 225, 826
CfoI GCGC 1 cut(s) 45
Cfr13I GGNCC 3 cut(s) 35, 272, 273
ClaI ATCGAT 2 cut(s) 207, 929
Csp6I GTAC 2 cut(s) 214, 482
CviAII CATG 5 cut(s) 180, 226, 827, 1150, 1222
CviQI GTAC 2 cut(s) 214, 482
DdeI CTNAG 7 cut(s) 144, 186, 341, 769, 887, 907, 1206
DpnI GATC 3 cut(s) 30, 265, 997
DpnII GATC 3 cut(s) 28, 263, 995
EaeI YGGCCR 2 cut(s) 664, 688
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
EciI GGCGGA 1 cut(s) 988
Eco24I GRGCYC 1 cut(s) 276
Eco31I GGTCTC 1 cut(s) 1209
Eco47I GGWCC 1 cut(s) 35
Eco57I CTGAAG 1 cut(s) 150
Eco81I CCTNAGG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 666
EcoT22I ATGCAT 1 cut(s) 181
EcoT38I GRGCYC 1 cut(s) 276
FaeI CATG 5 cut(s) 183, 229, 830, 1153, 1225
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FaqI GGGAC 3 cut(s) 62, 528, 621
FatI CATG 5 cut(s) 179, 225, 826, 1149, 1221
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 1 cut(s) 532
FblI GTMKAC 1 cut(s) 1154
Fnu4HI GCNGC 3 cut(s) 393, 672, 774
FokI GGATG 2 cut(s) 35, 523
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 3 cut(s) 393, 672, 774
FspBI CTAG 1 cut(s) 719
GlaI GCGC 1 cut(s) 44
GluI GCNGC 3 cut(s) 393, 672, 774
HaeIII GGCC 5 cut(s) 184, 274, 666, 690, 941
HapII CCGG 3 cut(s) 38, 639, 1014
HhaI GCGC 1 cut(s) 45
Hin1II CATG 5 cut(s) 183, 229, 830, 1153, 1225
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HinfI GANTC 8 cut(s) 125, 229, 492, 642, 810, 850, 931, 1193
HpaII CCGG 3 cut(s) 38, 639, 1014
HphI GGTGA 1 cut(s) 1210
Hpy166II GTNNAC 5 cut(s) 164, 216, 970, 1155, 1212
Hpy188I TCNGA 5 cut(s) 112, 130, 189, 364, 647
Hpy188III TCNNGA 6 cut(s) 226, 267, 656, 719, 827, 1258
Hpy8I GTNNAC 5 cut(s) 164, 216, 970, 1155, 1212
HpyAV CCTTC 6 cut(s) 117, 246, 251, 255, 377, 539
HpyCH4III ACNGT 3 cut(s) 871, 992, 1267
HpyCH4V TGCA 8 cut(s) 164, 179, 392, 536, 575, 806, 926, 1253
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 365, 687, 845
HpyF3I CTNAG 7 cut(s) 144, 186, 341, 769, 887, 907, 1206
Hsp92II CATG 5 cut(s) 183, 229, 830, 1153, 1225
HspAI GCGC 1 cut(s) 43
Kzo9I GATC 3 cut(s) 28, 263, 995
LmnI GCTCC 2 cut(s) 40, 290
Lsp1109I GCAGC 2 cut(s) 404, 658
LweI GCATC 2 cut(s) 404, 935
MaeI CTAG 1 cut(s) 719
MaeIII GTNAC 1 cut(s) 1024
MalI GATC 3 cut(s) 30, 265, 997
MboI GATC 3 cut(s) 28, 263, 995
MboII GAAGA 7 cut(s) 86, 285, 324, 809, 854, 1138, 1241
MfeI CAATTG 1 cut(s) 1029
MflI RGATCY 2 cut(s) 28, 995
MhlI GDGCHC 2 cut(s) 166, 276
MlsI TGGCCA 1 cut(s) 666
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 4 cut(s) 223, 636, 844, 1187
MmeI TCCRAC 1 cut(s) 892
Mox20I TGGCCA 1 cut(s) 666
Mph1103I ATGCAT 1 cut(s) 181
MscI TGGCCA 1 cut(s) 666
MseI TTAA 5 cut(s) 62, 303, 614, 695, 1004
MslI CAYNNNNRTG 3 cut(s) 209, 990, 1088
Msp20I TGGCCA 1 cut(s) 666
MspI CCGG 3 cut(s) 38, 639, 1014
MspR9I CCNGG 2 cut(s) 639, 668
MunI CAATTG 1 cut(s) 1029
MvaI CCWGG 1 cut(s) 668
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 4 cut(s) 161, 365, 687, 845
NciI CCSGG 1 cut(s) 639
NdeI CATATG 1 cut(s) 532
NdeII GATC 3 cut(s) 28, 263, 995
NlaIII CATG 5 cut(s) 183, 229, 830, 1153, 1225
NlaIV GGNNCC 3 cut(s) 274, 997, 1135
NmuCI GTSAC 1 cut(s) 1024
NsiI ATGCAT 1 cut(s) 181
NspI RCATGY 1 cut(s) 1225
PagI TCATGA 2 cut(s) 225, 826
PfeI GAWTC 4 cut(s) 125, 492, 810, 931
PflMI CCANNNNNTGG 2 cut(s) 662, 873
PfoI TCCNGGA 1 cut(s) 637
PkrI GCNGC 3 cut(s) 394, 673, 775
PleI GAGTC 4 cut(s) 223, 636, 844, 1187
PpsI GAGTC 4 cut(s) 223, 636, 844, 1187
PshBI ATTAAT 1 cut(s) 614
Psp6I CCWGG 1 cut(s) 666
PspGI CCWGG 1 cut(s) 666
PspN4I GGNNCC 3 cut(s) 274, 997, 1135
PspOMI GGGCCC 1 cut(s) 272
PspPI GGNCC 3 cut(s) 35, 272, 273
PsuI RGATCY 2 cut(s) 28, 995
RsaI GTAC 2 cut(s) 215, 483
RsaNI GTAC 2 cut(s) 214, 482
RseI CAYNNNNRTG 3 cut(s) 209, 990, 1088
SaqAI TTAA 5 cut(s) 62, 303, 614, 695, 1004
SatI GCNGC 3 cut(s) 393, 672, 774
Sau3AI GATC 3 cut(s) 28, 263, 995
Sau96I GGNCC 3 cut(s) 35, 272, 273
SchI GAGTC 4 cut(s) 223, 636, 844, 1187
ScrFI CCNGG 2 cut(s) 639, 668
SduI GDGCHC 2 cut(s) 166, 276
SfaNI GCATC 2 cut(s) 404, 935
SinI GGWCC 1 cut(s) 35
SmiMI CAYNNNNRTG 3 cut(s) 209, 990, 1088
SsiI CCGC 5 cut(s) 45, 417, 446, 773, 999
SspI AATATT 1 cut(s) 780
SspMI CTAG 1 cut(s) 719
StyD4I CCNGG 2 cut(s) 637, 666
TaaI ACNGT 3 cut(s) 871, 992, 1267
TaqI TCGA 2 cut(s) 207, 929
TatI WGTACW 2 cut(s) 213, 481
TauI GCSGC 1 cut(s) 776
TfiI GAWTC 4 cut(s) 125, 492, 810, 931
Tru1I TTAA 5 cut(s) 62, 303, 614, 695, 1004
Tru9I TTAA 5 cut(s) 62, 303, 614, 695, 1004
TscAI CASTG 3 cut(s) 874, 997, 1071
TseFI GTSAC 1 cut(s) 1024
TseI GCWGC 2 cut(s) 392, 671
Tsp45I GTSAC 1 cut(s) 1024
TspDTI ATGAA 5 cut(s) 87, 456, 843, 974, 1138
TspRI CASTG 3 cut(s) 874, 997, 1071
Van91I CCANNNNNTGG 2 cut(s) 662, 873
VneI GTGCAC 1 cut(s) 162
VpaK11BI GGWCC 1 cut(s) 35
VspI ATTAAT 1 cut(s) 614
XapI RAATTY 4 cut(s) 424, 606, 831, 1232
XbaI TCTAGA 1 cut(s) 718
XceI RCATGY 1 cut(s) 1225
XcmI CCANNNNNNNNNTGG 1 cut(s) 674
XmiI GTMKAC 1 cut(s) 1154
XspI CTAG 1 cut(s) 719
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.