Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0014381

Physical Location & Seq

Genomic Coordinates Reverse (-)

49457812 .. 49458731

Gene Structure 920 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00429100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 909 bp

ATGGCGGAAACCACCACCACCGCACGGCCCCTCAAAATCATCGCCGGCGCCGACTCCTTCGGCTGTTCACTCAAAGACGCCCTGGTCTCCCACCTCCGCTCCCTCAACATCGACGTCGAGGACCTCGGCACCTCCTCCTACTACTCCATCGCCGCCGACGTCGGCCGCCGCGTCTCCTCCTCCTCCGCCAACGTCGAAACTCGCGGACTCGTCGCCTGTGGCACTGGCGTCGGCGTCGCGATCTTCGCCAACAATCTTCGCCGCCACGTGTCTCTCCCCTCCGACGCCCTTAACGCCCGGTCCATCAACAACTCCAACGTCCTCGCCGTCTCCGGCATGTCGACCTCGCCGGAATCCGCCGTCGAGATCCTCGACACGTGGCTCAACACTCCCTTCAAAGCGCCGTGTCCGGCGTCGGAGTCGAAACCTTGGCCGGAAGAGATATCATCTTTTCTTGATAACTCAATGAATGACATGTCACAAATTGGAGCTGATTCTGATGTGAAGGAGAGCTCTTGCTCTATCTGCTGTTTAGTGAAGAATCGGAATTTGAATCCGATTGATATAATTCCGGGTGGATCCATGAAGATATTGAGGGAGACTCCCACATCGGCCATAGTTAGGTTTAAGGCCGGGAGCGTGGAGCCGGCTCATCACCACACTTTCGGGCACGATTTGGTTGTGTTGGAGGGGAAGAAGAGTGTGTGGAATTTAACTAAGAAGGAGAGATTTGATCTAGTTGTTGGGGATTACTTGTTTACTCCAGCTGGGGATGTGCATAGAGTCAAGTATTATGAAGATACCGAGTTCTTTATCAAGTGGGATGGGAAATGGGATATGTTCTTCGATGAAGATCTCGAGACTGCGAAGAAGGCTGTTGATCAAGCACTGGAGGATGAGGGTAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cupin_2 Cupin_7 LacAB_rpiB

Protein Analysis

302

Amino Acids

32.92

Weight (kDa)

5.15

Isoelectric Point (pI)

43.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LacAB_rpiB	PF02502	13 - 133	2.6e-20	Ribose/Galactose Isomerase
Cupin_7	PF12973	188 - 271	5.2e-07	ChrR Cupin-like domain
Cupin_2	PF07883	206 - 266	1.3e-06	Cupin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014381)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04880
fragaria_vesca	FvH4_6g03430
malus_domestica	MD12G1237200.v1.1
prunus_persica	Prupe.6G339900_v2.0.a1
pyrus_communis	pycom12g21810
rosa_chinensis	RchiOBHm_Chr3g0451931
rosa_laevigata	RLG00000025637
rosa_multiflora	Rmu_sc0002901.1_g000002 Rmu_ssc0000467.1_g000022
rosa_roxburghii	Rroxscaffold_164G00436200 Rroxscaffold_6G00429100
rosa_rugosa	Rorug02G0637500
rosa_samantha	Rh3AG039200 Rh3BG040500 Rh3CG039000 Rh3DG039700
rosa_wichuraiana	Rw3G002930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	83
AatII	GACGTC	2 cut(s)	117, 162
AccB1I	GGYRCC	2 cut(s)	47, 128
AccBSI	CCGCTC	1 cut(s)	99
AccI	GTMKAC	1 cut(s)	341
AccII	CGCG	3 cut(s)	171, 204, 239
AclWI	GGATC	3 cut(s)	361, 573, 586
AcoI	YGGCCR	3 cut(s)	163, 431, 612
AcsI	RAATTY	2 cut(s)	547, 709
AcvI	CACGTG	2 cut(s)	268, 378
AcyI	GRCGYC	8 cut(s)	48, 78, 114, 159, 228, 234, 285, 413
AfiI	CCNNNNNNNGG	2 cut(s)	24, 621
AflIII	ACRYGT	3 cut(s)	267, 375, 474
AgsI	TTSAA	2 cut(s)	397, 553
AjnI	CCWGG	1 cut(s)	81
AluBI	AGCT	3 cut(s)	491, 513, 767
AluI	AGCT	3 cut(s)	491, 513, 767
Alw21I	GWGCWC	1 cut(s)	515
Alw26I	GTCTC	6 cut(s)	91, 178, 276, 334, 593, 854
AlwI	GGATC	3 cut(s)	361, 573, 586
Ama87I	CYCGRG	1 cut(s)	857
AoxI	GGCC	5 cut(s)	26, 163, 431, 612, 630
ApoI	RAATTY	2 cut(s)	547, 709
AspLEI	GCGC	2 cut(s)	50, 403
AspS9I	GGNCC	3 cut(s)	27, 121, 300
AsuC2I	CCSGG	3 cut(s)	298, 573, 634
AsuHPI	GGTGA	1 cut(s)	647
AvaI	CYCGRG	1 cut(s)	857
AvaII	GGWCC	2 cut(s)	121, 300
BaeGI	GKGCMC	1 cut(s)	672
BamHI	GGATCC	1 cut(s)	578
BanI	GGYRCC	2 cut(s)	47, 128
BanII	GRGCYC	1 cut(s)	515
BbrPI	CACGTG	2 cut(s)	268, 378
Bbv12I	GWGCWC	1 cut(s)	515
BccI	CCATC	3 cut(s)	155, 311, 818
BceAI	ACGGC	4 cut(s)	41, 311, 344, 388
BcgI	CGANNNNNNTGC	2 cut(s)	211, 245
BciT130I	CCWGG	1 cut(s)	83
BclI	TGATCA	1 cut(s)	880
BcnI	CCSGG	3 cut(s)	298, 573, 634
BcoDI	GTCTC	6 cut(s)	91, 178, 276, 334, 593, 854
BfaI	CTAG	1 cut(s)	737
BfoI	RGCGCY	2 cut(s)	51, 404
BglII	AGATCT	1 cut(s)	853
BisI	GCNGC	4 cut(s)	153, 166, 169, 262
BlsI	GCNGC	4 cut(s)	154, 167, 170, 263
Bme1390I	CCNGG	4 cut(s)	83, 298, 573, 634
Bme18I	GGWCC	2 cut(s)	121, 300
BmeT110I	CYCGRG	1 cut(s)	857
BmgT120I	GGNCC	3 cut(s)	27, 121, 300
BmiI	GGNNCC	5 cut(s)	29, 49, 130, 580, 645
BmrFI	CCNGG	4 cut(s)	83, 298, 573, 634
BplI	GAGNNNNNCTC	2 cut(s)	586, 618
BpmI	CTGGAG	1 cut(s)	747
BpuMI	CCSGG	3 cut(s)	298, 573, 634
BsaAI	YACGTR	2 cut(s)	268, 378
BsaBI	GATNNNNATC	1 cut(s)	852
BsaHI	GRCGYC	8 cut(s)	48, 78, 114, 159, 228, 234, 285, 413
BsaI	GGTCTC	1 cut(s)	91
BsaJI	CCNNGG	3 cut(s)	81, 124, 428
BsaXI	ACNNNNNCTCC	2 cut(s)	83, 113
Bsc4I	CCNNNNNNNGG	2 cut(s)	24, 621
Bse118I	RCCGGY	2 cut(s)	44, 646
Bse1I	ACTGG	2 cut(s)	229, 894
Bse8I	GATNNNNATC	1 cut(s)	852
BseBI	CCWGG	1 cut(s)	83
BseDI	CCNNGG	3 cut(s)	81, 124, 428
BseGI	GGATG	3 cut(s)	778, 829, 901
BseJI	GATNNNNATC	1 cut(s)	852
BseLI	CCNNNNNNNGG	2 cut(s)	24, 621
BseNI	ACTGG	2 cut(s)	229, 894
BseRI	GAGGAG	4 cut(s)	124, 166, 169, 172
BseSI	GKGCMC	1 cut(s)	672
BseX3I	CGGCCG	1 cut(s)	163
BseYI	CCCAGC	1 cut(s)	767
Bsh1236I	CGCG	3 cut(s)	171, 204, 239
Bsh1285I	CGRYCG	1 cut(s)	166
BshFI	GGCC	5 cut(s)	28, 165, 433, 614, 632
BshNI	GGYRCC	2 cut(s)	47, 128
BsiEI	CGRYCG	1 cut(s)	166
BsiHKAI	GWGCWC	1 cut(s)	515
BsiHKCI	CYCGRG	1 cut(s)	857
BsiSI	CCGG	9 cut(s)	45, 298, 333, 350, 410, 434, 572, 633, 647
BslI	CCNNNNNNNGG	2 cut(s)	24, 621
BsmAI	GTCTC	6 cut(s)	91, 178, 276, 334, 593, 854
BsmBI	CGTCTC	2 cut(s)	178, 334
BsnI	GGCC	5 cut(s)	28, 165, 433, 614, 632
Bso31I	GGTCTC	1 cut(s)	91
BsoBI	CYCGRG	1 cut(s)	857
Bsp1286I	GDGCHC	2 cut(s)	515, 672
Bsp143I	GATC	6 cut(s)	240, 366, 578, 733, 853, 880
Bsp68I	TCGCGA	1 cut(s)	239
BspANI	GGCC	5 cut(s)	28, 165, 433, 614, 632
BspFNI	CGCG	3 cut(s)	171, 204, 239
BspLI	GGNNCC	5 cut(s)	29, 49, 130, 580, 645
BspPI	GGATC	3 cut(s)	361, 573, 586
BspT107I	GGYRCC	2 cut(s)	47, 128
BspTNI	GGTCTC	1 cut(s)	91
BsrBI	CCGCTC	1 cut(s)	99
BsrFI	RCCGGY	2 cut(s)	44, 646
BsrI	ACTGG	2 cut(s)	229, 894
BssAI	RCCGGY	2 cut(s)	44, 646
BssECI	CCNNGG	3 cut(s)	81, 124, 428
BssMI	GATC	6 cut(s)	240, 366, 578, 733, 853, 880
BssNI	GRCGYC	8 cut(s)	48, 78, 114, 159, 228, 234, 285, 413
BssT1I	CCWWGG	1 cut(s)	428
Bst2UI	CCWGG	1 cut(s)	83
Bst6I	CTCTTC	2 cut(s)	432, 692
BstACI	GRCGYC	8 cut(s)	48, 78, 114, 159, 228, 234, 285, 413
BstBAI	YACGTR	2 cut(s)	268, 378
BstC8I	GCNNGC	2 cut(s)	46, 648
BstDEI	CTNAG	1 cut(s)	717
BstF5I	GGATG	3 cut(s)	778, 829, 901
BstFNI	CGCG	3 cut(s)	171, 204, 239
BstH2I	RGCGCY	2 cut(s)	51, 404
BstHHI	GCGC	2 cut(s)	50, 403
BstKTI	GATC	6 cut(s)	243, 369, 581, 736, 856, 883
BstMAI	GTCTC	6 cut(s)	91, 178, 276, 334, 593, 854
BstMBI	GATC	6 cut(s)	240, 366, 578, 733, 853, 880
BstMCI	CGRYCG	1 cut(s)	166
BstMWI	GCNNNNNNNGC	4 cut(s)	245, 293, 525, 872
BstNI	CCWGG	1 cut(s)	83
BstNSI	RCATGY	2 cut(s)	340, 478
BstSCI	CCNGG	4 cut(s)	81, 296, 571, 632
BstSLI	GKGCMC	1 cut(s)	672
BstUI	CGCG	3 cut(s)	171, 204, 239
BstX2I	RGATCY	3 cut(s)	366, 578, 853
BstYI	RGATCY	3 cut(s)	366, 578, 853
BstZI	CGGCCG	1 cut(s)	163
BsuRI	GGCC	5 cut(s)	28, 165, 433, 614, 632
BtgZI	GCGATG	2 cut(s)	25, 133
BtsCI	GGATG	3 cut(s)	778, 829, 901
BtsIMutI	CAGTG	2 cut(s)	222, 887
BtuMI	TCGCGA	1 cut(s)	239
Cac8I	GCNNGC	2 cut(s)	46, 648
CfoI	GCGC	2 cut(s)	50, 403
Cfr10I	RCCGGY	2 cut(s)	44, 646
Cfr13I	GGNCC	3 cut(s)	27, 121, 300
CseI	GACGC	6 cut(s)	86, 160, 217, 223, 293, 402
CviAII	CATG	3 cut(s)	337, 475, 583
DdeI	CTNAG	1 cut(s)	717
DinI	GGCGCC	1 cut(s)	49
DpnI	GATC	6 cut(s)	242, 368, 580, 735, 855, 882
DpnII	GATC	6 cut(s)	240, 366, 578, 733, 853, 880
DrdI	GACNNNNNNGTC	1 cut(s)	83
DseDI	GACNNNNNNGTC	1 cut(s)	83
EaeI	YGGCCR	3 cut(s)	163, 431, 612
EagI	CGGCCG	1 cut(s)	163
Eam1104I	CTCTTC	2 cut(s)	432, 692
EarI	CTCTTC	2 cut(s)	432, 692
EciI	GGCGGA	3 cut(s)	20, 175, 346
Ecl136II	GAGCTC	1 cut(s)	513
EclXI	CGGCCG	1 cut(s)	163
Eco130I	CCWWGG	1 cut(s)	428
Eco24I	GRGCYC	1 cut(s)	515
Eco31I	GGTCTC	1 cut(s)	91
Eco32I	GATATC	1 cut(s)	444
Eco47I	GGWCC	2 cut(s)	121, 300
Eco52I	CGGCCG	1 cut(s)	163
Eco53kI	GAGCTC	1 cut(s)	513
Eco72I	CACGTG	2 cut(s)	268, 378
Eco88I	CYCGRG	1 cut(s)	857
EcoICRI	GAGCTC	1 cut(s)	513
EcoO109I	RGGNCCY	1 cut(s)	121
EcoRII	CCWGG	1 cut(s)	81
EcoRV	GATATC	1 cut(s)	444
EcoT14I	CCWWGG	1 cut(s)	428
EcoT38I	GRGCYC	1 cut(s)	515
EgeI	GGCGCC	1 cut(s)	49
EheI	GGCGCC	1 cut(s)	49
ErhI	CCWWGG	1 cut(s)	428
Esp3I	CGTCTC	2 cut(s)	178, 334
FaeI	CATG	3 cut(s)	340, 478, 586
FaiI	YATR	8 cut(s)	338, 476, 566, 584, 617, 780, 795, 839
FatI	CATG	3 cut(s)	336, 474, 582
FbaI	TGATCA	1 cut(s)	880
FblI	GTMKAC	1 cut(s)	341
Fnu4HI	GCNGC	4 cut(s)	153, 166, 169, 262
FokI	GGATG	2 cut(s)	785, 836
FriOI	GRGCYC	1 cut(s)	515
Fsp4HI	GCNGC	4 cut(s)	153, 166, 169, 262
FspBI	CTAG	1 cut(s)	737
GlaI	GCGC	2 cut(s)	49, 402
GluI	GCNGC	4 cut(s)	153, 166, 169, 262
GsaI	CCCAGC	1 cut(s)	771
GsuI	CTGGAG	1 cut(s)	747
HaeII	RGCGCY	2 cut(s)	51, 404
HaeIII	GGCC	5 cut(s)	28, 165, 433, 614, 632
HapII	CCGG	9 cut(s)	45, 298, 333, 350, 410, 434, 572, 633, 647
HgaI	GACGC	6 cut(s)	86, 160, 217, 223, 293, 402
HhaI	GCGC	2 cut(s)	50, 403
Hin1I	GRCGYC	8 cut(s)	48, 78, 114, 159, 228, 234, 285, 413
Hin1II	CATG	3 cut(s)	340, 478, 586
Hin6I	GCGC	2 cut(s)	48, 401
HinP1I	GCGC	2 cut(s)	48, 401
HincII	GTYRAC	1 cut(s)	342
HindII	GTYRAC	1 cut(s)	342
HinfI	GANTC	9 cut(s)	53, 207, 353, 419, 494, 541, 553, 601, 783
HpaII	CCGG	9 cut(s)	45, 298, 333, 350, 410, 434, 572, 633, 647
HphI	GGTGA	1 cut(s)	647
Hpy166II	GTNNAC	3 cut(s)	68, 342, 759
Hpy188I	TCNGA	5 cut(s)	283, 418, 499, 546, 558
Hpy188III	TCNNGA	5 cut(s)	238, 364, 455, 857, 859
Hpy8I	GTNNAC	3 cut(s)	68, 342, 759
HpyAV	CCTTC	5 cut(s)	67, 403, 499, 715, 865
HpyCH4IV	ACGT	6 cut(s)	114, 159, 192, 267, 318, 377
HpyCH4V	TGCA	1 cut(s)	778
HpyF10VI	GCNNNNNNNGC	4 cut(s)	245, 293, 525, 872
HpyF3I	CTNAG	1 cut(s)	717
HpySE526I	ACGT	6 cut(s)	114, 159, 192, 267, 318, 377
Hsp92I	GRCGYC	8 cut(s)	48, 78, 114, 159, 228, 234, 285, 413
Hsp92II	CATG	3 cut(s)	340, 478, 586
HspAI	GCGC	2 cut(s)	48, 401
KasI	GGCGCC	1 cut(s)	47
KroI	GCCGGC	2 cut(s)	44, 646
KroNI	GCCGGC	2 cut(s)	46, 648
Ksp22I	TGATCA	1 cut(s)	880
Kzo9I	GATC	6 cut(s)	240, 366, 578, 733, 853, 880
LmnI	GCTCC	4 cut(s)	104, 488, 636, 643
MaeI	CTAG	1 cut(s)	737
MaeII	ACGT	6 cut(s)	114, 159, 192, 267, 318, 377
MaeIII	GTNAC	1 cut(s)	477
MalI	GATC	6 cut(s)	242, 368, 580, 735, 855, 882
MbiI	CCGCTC	1 cut(s)	99
MboI	GATC	6 cut(s)	240, 366, 578, 733, 853, 880
MflI	RGATCY	3 cut(s)	366, 578, 853
MhlI	GDGCHC	2 cut(s)	515, 672
MluCI	AATT	4 cut(s)	483, 547, 567, 709
Mly113I	GGCGCC	1 cut(s)	48
MlyI	GAGTC	5 cut(s)	47, 201, 428, 595, 792
MmeI	TCCRAC	4 cut(s)	306, 339, 396, 666
MreI	CGCCGGCG	1 cut(s)	44
MroNI	GCCGGC	2 cut(s)	44, 646
MseI	TTAA	3 cut(s)	291, 627, 713
MspA1I	CMGCKG	1 cut(s)	767
MspI	CCGG	9 cut(s)	45, 298, 333, 350, 410, 434, 572, 633, 647
MspR9I	CCNGG	4 cut(s)	83, 298, 573, 634
MvaI	CCWGG	1 cut(s)	83
MvnI	CGCG	3 cut(s)	171, 204, 239
MwoI	GCNNNNNNNGC	4 cut(s)	245, 293, 525, 872
NaeI	GCCGGC	2 cut(s)	46, 648
NarI	GGCGCC	1 cut(s)	48
NciI	CCSGG	3 cut(s)	298, 573, 634
NdeII	GATC	6 cut(s)	240, 366, 578, 733, 853, 880
NgoMIV	GCCGGC	2 cut(s)	44, 646
NlaIII	CATG	3 cut(s)	340, 478, 586
NlaIV	GGNNCC	5 cut(s)	29, 49, 130, 580, 645
NmeAIII	GCCGAG	1 cut(s)	105
NmuCI	GTSAC	1 cut(s)	477
NruI	TCGCGA	1 cut(s)	239
NspI	RCATGY	2 cut(s)	340, 478
PaeR7I	CTCGAG	1 cut(s)	857
PciI	ACATGT	1 cut(s)	474
PcsI	WCGNNNNNNNCGW	5 cut(s)	48, 156, 168, 324, 369
PdiI	GCCGGC	2 cut(s)	46, 648
PfeI	GAWTC	4 cut(s)	353, 494, 541, 553
PkrI	GCNGC	4 cut(s)	154, 167, 170, 263
PleI	GAGTC	5 cut(s)	47, 201, 427, 595, 791
PluTI	GGCGCC	1 cut(s)	51
PmaCI	CACGTG	2 cut(s)	268, 378
PmlI	CACGTG	2 cut(s)	268, 378
PpsI	GAGTC	5 cut(s)	47, 201, 427, 595, 791
Ppu21I	YACGTR	2 cut(s)	268, 378
PpuMI	RGGWCCY	1 cut(s)	121
PscI	ACATGT	1 cut(s)	474
Psp124BI	GAGCTC	1 cut(s)	515
Psp5II	RGGWCCY	1 cut(s)	121
Psp6I	CCWGG	1 cut(s)	81
PspCI	CACGTG	2 cut(s)	268, 378
PspFI	CCCAGC	1 cut(s)	767
PspGI	CCWGG	1 cut(s)	81
PspN4I	GGNNCC	5 cut(s)	29, 49, 130, 580, 645
PspPI	GGNCC	3 cut(s)	27, 121, 300
PspPPI	RGGWCCY	1 cut(s)	121
PsuI	RGATCY	3 cut(s)	366, 578, 853
PvuII	CAGCTG	1 cut(s)	767
RruI	TCGCGA	1 cut(s)	239
SacI	GAGCTC	1 cut(s)	515
SalI	GTCGAC	1 cut(s)	340
SaqAI	TTAA	3 cut(s)	291, 627, 713
SatI	GCNGC	4 cut(s)	153, 166, 169, 262
Sau3AI	GATC	6 cut(s)	240, 366, 578, 733, 853, 880
Sau96I	GGNCC	3 cut(s)	27, 121, 300
SchI	GAGTC	5 cut(s)	47, 201, 428, 595, 792
ScrFI	CCNGG	4 cut(s)	83, 298, 573, 634
SduI	GDGCHC	2 cut(s)	515, 672
SfoI	GGCGCC	1 cut(s)	49
Sfr274I	CTCGAG	1 cut(s)	857
SgrAI	CRCCGGYG	1 cut(s)	44
SinI	GGWCC	2 cut(s)	121, 300
SlaI	CTCGAG	1 cut(s)	857
SmlI	CTYRAG	1 cut(s)	857
SmoI	CTYRAG	1 cut(s)	857
Sse9I	AATT	4 cut(s)	483, 547, 567, 709
SspDI	GGCGCC	1 cut(s)	47
SspMI	CTAG	1 cut(s)	737
SstI	GAGCTC	1 cut(s)	515
StyD4I	CCNGG	4 cut(s)	81, 296, 571, 632
StyI	CCWWGG	1 cut(s)	428
TaiI	ACGT	6 cut(s)	117, 162, 195, 270, 321, 380
TaqI	TCGA	9 cut(s)	111, 117, 195, 341, 363, 372, 422, 846, 858
TasI	AATT	4 cut(s)	483, 547, 567, 709
TauI	GCSGC	4 cut(s)	155, 168, 171, 264
TfiI	GAWTC	4 cut(s)	353, 494, 541, 553
Tru1I	TTAA	3 cut(s)	291, 627, 713
Tru9I	TTAA	3 cut(s)	291, 627, 713
TscAI	CASTG	2 cut(s)	229, 894
TseFI	GTSAC	1 cut(s)	477
Tsp45I	GTSAC	1 cut(s)	477
TspDTI	ATGAA	4 cut(s)	482, 599, 810, 864
TspRI	CASTG	2 cut(s)	229, 894
VpaK11BI	GGWCC	2 cut(s)	121, 300
XapI	RAATTY	2 cut(s)	547, 709
XceI	RCATGY	2 cut(s)	340, 478
XhoI	CTCGAG	1 cut(s)	857
XmiI	GTMKAC	1 cut(s)	341
XspI	CTAG	1 cut(s)	737
ZraI	GACGTC	2 cut(s)	115, 160

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.