Rroxscaffold_7G00158250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003623
Physical Location & Seq
Reverse (-)
1743108 .. 1746412
3305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00158250.1

Sequence Viewer

Length: 747 bp
ATGCTCCTCTCTCAGATTGCAGAAAGCGAGGATGAGAGAATAGTTAGAGAGTTTGCACTATTGGTGGATGCATATGAAGCCTACACTAATACAAGCGGAAGTACCACCAAAGAAGAAGGTGGGGAGAAATCCACCATTTCCGAAAGATTCATGGGGATGAAAGCTGCTATAGTTACAAACAATTCAAATTTGAAGTCTTTTGGTACCAAACTTGGTTTCAGCGTGTTGCCAAATGATGGTTTTATCAAAAGCAGTGAGGCCCAAGCTGAGCTGCCAGTTGATACTGTGGCATCTGAATTGCTGAAGTTGCTTGGATTTCAAGAAGGAAAGACACTGGAGTCGAGTCGGTATGATTTTGTGTTTGTGCGTGTTGGGGCTGGTGAGGTGAATGTTAAGAATGACAAGGCTGTTGCAGATGATGTGGAGTATCCCAATGCTTTGATTCGTGCTATAATGAATATAGGCAAACCTGGAACTGAAATCAGTCTCCGTTTGCACTTGTCTGTTGTAATGAGCTATGGGGGTACCTCGGAGAAAGACGATCCCGACTTGTCGTTTTCAATCAGTAAAGATAACTCCAATCTTTCAAAGCTCGTTCCTCACCAAAGTTATACCATGAAAGGAGAGGTTCCGCGGAAGGATGTCAGGCACCATTCCCCAATGTTACTGGCTCAGTGGCAATATGCTGTGACCCGCAAGGACATGGCAGAGACATTCTCTTTTAAAGATTTCAAGGAGCTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

248

Amino Acids

27.51

Weight (kDa)

5.27

Isoelectric Point (pI)

23.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 337
Acc65I GGTACC 2 cut(s) 203, 524
AccB1I GGYRCC 3 cut(s) 203, 524, 648
AccB7I CCANNNNNTGG 1 cut(s) 236
AccII CGCG 1 cut(s) 634
AciI CCGC 4 cut(s) 96, 632, 634, 694
AclWI GGATC 1 cut(s) 536
AcsI RAATTY 1 cut(s) 187
AcuI CTGAAG 1 cut(s) 323
AfaI GTAC 3 cut(s) 103, 205, 526
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 6 cut(s) 186, 193, 320, 561, 588, 733
AjnI CCWGG 1 cut(s) 469
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 6 cut(s) 164, 266, 271, 516, 592, 739
AluI AGCT 6 cut(s) 164, 266, 271, 516, 592, 739
Alw21I GWGCWC 1 cut(s) 741
Alw26I GTCTC 2 cut(s) 491, 704
AlwI GGATC 1 cut(s) 536
AoxI GGCC 1 cut(s) 258
ApeKI GCWGC 2 cut(s) 164, 271
ApoI RAATTY 1 cut(s) 187
ArsI GACNNNNNNTTYG 2 cut(s) 179, 211
Asp718I GGTACC 2 cut(s) 203, 524
AspS9I GGNCC 1 cut(s) 259
AsuHPI GGTGA 3 cut(s) 392, 397, 593
BanI GGYRCC 3 cut(s) 203, 524, 648
BanII GRGCYC 1 cut(s) 741
Bbv12I GWGCWC 1 cut(s) 741
BbvI GCAGC 2 cut(s) 151, 258
BccI CCATC 1 cut(s) 230
BciT130I CCWGG 1 cut(s) 471
BciVI GTATCC 1 cut(s) 438
BcoDI GTCTC 2 cut(s) 491, 704
BfaI CTAG 1 cut(s) 745
BfmI CTRYAG 1 cut(s) 168
BfuI GTATCC 1 cut(s) 438
BisI GCNGC 2 cut(s) 165, 272
BlpI GCTNAGC 1 cut(s) 267
BlsI GCNGC 2 cut(s) 166, 273
Bme1390I CCNGG 1 cut(s) 471
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 4 cut(s) 205, 526, 630, 650
BmrFI CCNGG 1 cut(s) 471
BmsI GCATC 2 cut(s) 58, 299
BplI GAGNNNNNCTC 2 cut(s) 701, 733
BpmI CTGGAG 1 cut(s) 356
Bpu1102I GCTNAGC 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 528, 632
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse1I ACTGG 3 cut(s) 275, 339, 672
BseBI CCWGG 1 cut(s) 471
BseDI CCNNGG 2 cut(s) 528, 632
BseGI GGATG 4 cut(s) 37, 73, 162, 646
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMII CTCAG 3 cut(s) 26, 258, 686
BseNI ACTGG 3 cut(s) 275, 339, 672
BseXI GCAGC 2 cut(s) 151, 258
Bsh1236I CGCG 1 cut(s) 634
BshFI GGCC 1 cut(s) 260
BshNI GGYRCC 3 cut(s) 203, 524, 648
BsiHKAI GWGCWC 1 cut(s) 741
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 2 cut(s) 491, 704
BsnI GGCC 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 741
Bsp143I GATC 1 cut(s) 541
Bsp1720I GCTNAGC 1 cut(s) 267
BspACI CCGC 4 cut(s) 96, 632, 634, 694
BspANI GGCC 1 cut(s) 260
BspCNI CTCAG 3 cut(s) 25, 259, 685
BspFNI CGCG 1 cut(s) 634
BspLI GGNNCC 4 cut(s) 205, 526, 630, 650
BspPI GGATC 1 cut(s) 536
BspT107I GGYRCC 3 cut(s) 203, 524, 648
BsrI ACTGG 3 cut(s) 275, 339, 672
BssECI CCNNGG 2 cut(s) 528, 632
BssMI GATC 1 cut(s) 541
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 1 cut(s) 286
BstDEI CTNAG 3 cut(s) 12, 267, 672
BstDSI CCRYGG 1 cut(s) 632
BstF5I GGATG 4 cut(s) 37, 73, 162, 646
BstFNI CGCG 1 cut(s) 634
BstKTI GATC 1 cut(s) 544
BstMAI GTCTC 2 cut(s) 491, 704
BstMBI GATC 1 cut(s) 541
BstMWI GCNNNNNNNGC 2 cut(s) 77, 307
BstNI CCWGG 1 cut(s) 471
BstSCI CCNGG 1 cut(s) 469
BstSFI CTRYAG 1 cut(s) 168
BstUI CGCG 1 cut(s) 634
BstV1I GCAGC 2 cut(s) 151, 258
BsuI GTATCC 1 cut(s) 438
BsuRI GGCC 1 cut(s) 260
BtgI CCRYGG 1 cut(s) 632
BtsCI GGATG 4 cut(s) 37, 73, 162, 646
BtsI GCAGTG 1 cut(s) 259
BtsIMutI CAGTG 3 cut(s) 259, 332, 680
Cfr13I GGNCC 1 cut(s) 259
Cfr42I CCGCGG 1 cut(s) 635
Csp6I GTAC 3 cut(s) 102, 204, 525
CviAII CATG 3 cut(s) 151, 616, 703
CviQI GTAC 3 cut(s) 102, 204, 525
DdeI CTNAG 3 cut(s) 12, 267, 672
DpnI GATC 1 cut(s) 543
DpnII GATC 1 cut(s) 541
DraI TTTAAA 1 cut(s) 724
DrdI GACNNNNNNGTC 1 cut(s) 337
DseDI GACNNNNNNGTC 1 cut(s) 337
Ecl136II GAGCTC 1 cut(s) 739
Eco24I GRGCYC 1 cut(s) 741
Eco53kI GAGCTC 1 cut(s) 739
Eco57I CTGAAG 1 cut(s) 323
EcoICRI GAGCTC 1 cut(s) 739
EcoRII CCWGG 1 cut(s) 469
EcoT22I ATGCAT 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 741
FaeI CATG 3 cut(s) 154, 619, 706
FalI AAGNNNNNCTT 1 cut(s) 725
FatI CATG 3 cut(s) 150, 615, 702
FauI CCCGC 1 cut(s) 701
FauNDI CATATG 1 cut(s) 73
Fnu4HI GCNGC 2 cut(s) 165, 272
FokI GGATG 4 cut(s) 44, 80, 169, 653
FriOI GRGCYC 1 cut(s) 741
Fsp4HI GCNGC 2 cut(s) 165, 272
FspBI CTAG 1 cut(s) 745
GluI GCNGC 2 cut(s) 165, 272
GsuI CTGGAG 1 cut(s) 356
HaeIII GGCC 1 cut(s) 260
Hin1II CATG 3 cut(s) 154, 619, 706
HinfI GANTC 4 cut(s) 147, 338, 343, 442
HphI GGTGA 3 cut(s) 392, 397, 593
Hpy188I TCNGA 4 cut(s) 15, 142, 295, 532
Hpy188III TCNNGA 2 cut(s) 320, 545
HpyAV CCTTC 3 cut(s) 110, 317, 631
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 5 cut(s) 20, 56, 71, 413, 496
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 307
HpyF3I CTNAG 3 cut(s) 12, 267, 672
Hsp92II CATG 3 cut(s) 154, 619, 706
KpnI GGTACC 2 cut(s) 207, 528
KspI CCGCGG 1 cut(s) 635
Kzo9I GATC 1 cut(s) 541
LmnI GCTCC 2 cut(s) 9, 736
LpnPI CCDG 7 cut(s) 288, 320, 363, 456, 483, 631, 653
Lsp1109I GCAGC 2 cut(s) 151, 258
LweI GCATC 2 cut(s) 58, 299
MaeI CTAG 1 cut(s) 745
MaeIII GTNAC 3 cut(s) 172, 663, 688
MalI GATC 1 cut(s) 543
MboI GATC 1 cut(s) 541
MboII GAAGA 2 cut(s) 125, 733
MhlI GDGCHC 1 cut(s) 741
MluCI AATT 3 cut(s) 181, 187, 296
MlyI GAGTC 2 cut(s) 347, 352
MnlI CCTC 7 cut(s) 17, 22, 250, 376, 538, 609, 619
Mph1103I ATGCAT 1 cut(s) 73
MseI TTAA 2 cut(s) 393, 723
MslI CAYNNNNRTG 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 634
MspR9I CCNGG 1 cut(s) 471
MvaI CCWGG 1 cut(s) 471
MvnI CGCG 1 cut(s) 634
MwoI GCNNNNNNNGC 2 cut(s) 77, 307
NdeI CATATG 1 cut(s) 73
NdeII GATC 1 cut(s) 541
NlaIII CATG 3 cut(s) 154, 619, 706
NlaIV GGNNCC 4 cut(s) 205, 526, 630, 650
NmuCI GTSAC 1 cut(s) 688
NsiI ATGCAT 1 cut(s) 73
PfeI GAWTC 2 cut(s) 147, 442
PflMI CCANNNNNTGG 1 cut(s) 236
PkrI GCNGC 2 cut(s) 166, 273
PleI GAGTC 2 cut(s) 346, 351
PpsI GAGTC 2 cut(s) 346, 351
Psp124BI GAGCTC 1 cut(s) 741
Psp6I CCWGG 1 cut(s) 469
PspGI CCWGG 1 cut(s) 469
PspN4I GGNNCC 4 cut(s) 205, 526, 630, 650
PspPI GGNCC 1 cut(s) 259
RsaI GTAC 3 cut(s) 103, 205, 526
RsaNI GTAC 3 cut(s) 102, 204, 525
RseI CAYNNNNRTG 1 cut(s) 155
SacI GAGCTC 1 cut(s) 741
SacII CCGCGG 1 cut(s) 635
SaqAI TTAA 2 cut(s) 393, 723
SatI GCNGC 2 cut(s) 165, 272
Sau3AI GATC 1 cut(s) 541
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 2 cut(s) 347, 352
ScrFI CCNGG 1 cut(s) 471
SduI GDGCHC 1 cut(s) 741
SfaNI GCATC 2 cut(s) 58, 299
SfcI CTRYAG 1 cut(s) 168
Sfr303I CCGCGG 1 cut(s) 635
SgrBI CCGCGG 1 cut(s) 635
SmiMI CAYNNNNRTG 1 cut(s) 155
Sse9I AATT 3 cut(s) 181, 187, 296
SsiI CCGC 4 cut(s) 96, 632, 634, 694
SspMI CTAG 1 cut(s) 745
SstI GAGCTC 1 cut(s) 741
StyD4I CCNGG 1 cut(s) 469
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 1 cut(s) 341
TasI AATT 3 cut(s) 181, 187, 296
TfiI GAWTC 2 cut(s) 147, 442
Tru1I TTAA 2 cut(s) 393, 723
Tru9I TTAA 2 cut(s) 393, 723
TscAI CASTG 3 cut(s) 259, 339, 680
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 2 cut(s) 164, 271
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 5 cut(s) 90, 139, 173, 470, 632
TspGWI ACGGA 1 cut(s) 479
TspRI CASTG 3 cut(s) 259, 339, 680
Van91I CCANNNNNTGG 1 cut(s) 236
XapI RAATTY 1 cut(s) 187
XspI CTAG 1 cut(s) 745
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.