Rroxscaffold_7G00160260

acetyltransferase At3g50280-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016984
Physical Location & Seq
Forward (+)
3257611 .. 3258769
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00160260.1

Sequence Viewer

Length: 582 bp
ATGGGGAGCAGCGACGATGCGGAGGAGACGGGGAGCTTCGAGGACGCGGCGGTGAGGGAGCGCGAGGATGCGGCGGTGAGATGGGAGCAGGAAGCCGTGATGCGCGGCCGTGAATCCGTGATGAGGGAGAGAGATGGGAGCCGTGATGAGGTAGAGAGATGGGAGCAGGAGAGAGACGGGGAACGAGTGACCTTGATTCGTTATAAGCGTAGGGTTAATGGTTCGGTGAAATTGTTTCGACTTGGAAGGGGCGATCAACCACGACGGCCACACCATGTCACCGCCGACGGCTTAAGTCACGGGGTGGTGGACGGCGTTTTCATCGGGCTATCAATGAATCATGCGGTGGGAGACGGAACATCTTACCGGCATTTCTTCAACACTTGGTCCGAAATTTTCCAAGCACAAGAAAAGAATGTCCCGATTTCGAGAATATTTCCAACCCACCAGTGTTGGATCGGTGGTTTTCGGAAGGTCGTGGTCCGATTATCACCGCCTTTCACCGACCCAAGTGAGTTCATTGACGATTCGAAGCACCAAAACTCGGAGACAGAATGTTTCACTTCTCCGCAGAATCCCTAG

Protein Analysis

193

Amino Acids

22.14

Weight (kDa)

5.68

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 105 - 139 3.5e-06 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 204
AccII CGCG 3 cut(s) 47, 63, 105
AclWI GGATC 1 cut(s) 464
AcoI YGGCCR 2 cut(s) 106, 266
AcsI RAATTY 1 cut(s) 393
AdeI CACNNNGTG 1 cut(s) 304
AfiI CCNNNNNNNGG 3 cut(s) 123, 148, 544
AflII CTTAAG 1 cut(s) 292
AgsI TTSAA 1 cut(s) 379
AluBI AGCT 1 cut(s) 36
AluI AGCT 1 cut(s) 36
Alw26I GTCTC 4 cut(s) 20, 168, 345, 542
AlwI GGATC 1 cut(s) 464
AoxI GGCC 2 cut(s) 106, 266
ApeKI GCWGC 1 cut(s) 9
ApoI RAATTY 1 cut(s) 393
AspLEI GCGC 2 cut(s) 63, 105
AspS9I GGNCC 2 cut(s) 387, 481
AsuHPI GGTGA 6 cut(s) 64, 88, 238, 271, 483, 493
AsuII TTCGAA 1 cut(s) 530
AvaII GGWCC 2 cut(s) 387, 481
BbvI GCAGC 1 cut(s) 21
BccI CCATC 3 cut(s) 75, 128, 153
BceAI ACGGC 6 cut(s) 80, 93, 126, 281, 304, 328
BcoDI GTCTC 4 cut(s) 20, 168, 345, 542
BfaI CTAG 1 cut(s) 580
BfrI CTTAAG 1 cut(s) 292
BisI GCNGC 4 cut(s) 10, 48, 72, 106
BlsI GCNGC 4 cut(s) 11, 49, 73, 107
Bme18I GGWCC 2 cut(s) 387, 481
BmgT120I GGNCC 2 cut(s) 387, 481
BmiI GGNNCC 1 cut(s) 140
BmsI GCATC 3 cut(s) 7, 58, 90
Bpu14I TTCGAA 1 cut(s) 530
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 148, 544
Bse118I RCCGGY 1 cut(s) 366
Bse1I ACTGG 1 cut(s) 448
BseGI GGATG 1 cut(s) 73
BseLI CCNNNNNNNGG 3 cut(s) 123, 148, 544
BseNI ACTGG 1 cut(s) 448
BseRI GAGGAG 1 cut(s) 38
BseX3I CGGCCG 1 cut(s) 106
BseXI GCAGC 1 cut(s) 21
Bsh1236I CGCG 3 cut(s) 47, 63, 105
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 2 cut(s) 108, 268
BsiEI CGRYCG 1 cut(s) 109
BsiSI CCGG 1 cut(s) 367
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 3 cut(s) 123, 148, 544
BsmAI GTCTC 4 cut(s) 20, 168, 345, 542
BsmBI CGTCTC 3 cut(s) 20, 168, 345
BsmFI GGGAC 1 cut(s) 404
BsnI GGCC 2 cut(s) 108, 268
Bsp119I TTCGAA 1 cut(s) 530
Bsp143I GATC 2 cut(s) 253, 456
BspANI GGCC 2 cut(s) 108, 268
BspFNI CGCG 3 cut(s) 47, 63, 105
BspLI GGNNCC 1 cut(s) 140
BspPI GGATC 1 cut(s) 464
BspT104I TTCGAA 1 cut(s) 530
BspTI CTTAAG 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 366
BsrI ACTGG 1 cut(s) 448
BssAI RCCGGY 1 cut(s) 366
BssMI GATC 2 cut(s) 253, 456
BstAFI CTTAAG 1 cut(s) 292
BstBI TTCGAA 1 cut(s) 530
BstF5I GGATG 1 cut(s) 73
BstFNI CGCG 3 cut(s) 47, 63, 105
BstHHI GCGC 2 cut(s) 63, 105
BstKTI GATC 2 cut(s) 256, 459
BstMAI GTCTC 4 cut(s) 20, 168, 345, 542
BstMBI GATC 2 cut(s) 253, 456
BstMCI CGRYCG 1 cut(s) 109
BstUI CGCG 3 cut(s) 47, 63, 105
BstV1I GCAGC 1 cut(s) 21
BstZI CGGCCG 1 cut(s) 106
BsuRI GGCC 2 cut(s) 108, 268
BtsCI GGATG 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 455
CfoI GCGC 2 cut(s) 63, 105
Cfr10I RCCGGY 1 cut(s) 366
Cfr13I GGNCC 2 cut(s) 387, 481
CseI GACGC 1 cut(s) 53
CspCI CAANNNNNGTGG 2 cut(s) 434, 469
CviAII CATG 2 cut(s) 275, 341
CviJI RGCY 7 cut(s) 36, 95, 108, 141, 268, 291, 328
CviKI_1 RGCY 7 cut(s) 36, 95, 108, 141, 268, 291, 328
DpnI GATC 2 cut(s) 255, 458
DpnII GATC 2 cut(s) 253, 456
DraIII CACNNNGTG 1 cut(s) 304
EaeI YGGCCR 2 cut(s) 106, 266
EagI CGGCCG 1 cut(s) 106
EclXI CGGCCG 1 cut(s) 106
Eco47I GGWCC 2 cut(s) 387, 481
Eco52I CGGCCG 1 cut(s) 106
Esp3I CGTCTC 3 cut(s) 20, 168, 345
FaeI CATG 2 cut(s) 278, 344
FaiI YATR 3 cut(s) 204, 276, 342
FaqI GGGAC 1 cut(s) 404
FatI CATG 2 cut(s) 274, 340
Fnu4HI GCNGC 4 cut(s) 10, 48, 72, 106
FokI GGATG 1 cut(s) 80
Fsp4HI GCNGC 4 cut(s) 10, 48, 72, 106
FspBI CTAG 1 cut(s) 580
GlaI GCGC 2 cut(s) 62, 104
GluI GCNGC 4 cut(s) 10, 48, 72, 106
HaeIII GGCC 2 cut(s) 108, 268
HapII CCGG 1 cut(s) 367
HgaI GACGC 1 cut(s) 53
HhaI GCGC 2 cut(s) 63, 105
Hin1II CATG 2 cut(s) 278, 344
Hin6I GCGC 2 cut(s) 61, 103
HinP1I GCGC 2 cut(s) 61, 103
HinfI GANTC 5 cut(s) 113, 196, 337, 527, 574
HpaII CCGG 1 cut(s) 367
HphI GGTGA 6 cut(s) 64, 88, 238, 271, 483, 493
Hpy166II GTNNAC 1 cut(s) 310
Hpy188I TCNGA 4 cut(s) 391, 471, 485, 547
Hpy188III TCNNGA 2 cut(s) 421, 429
Hpy8I GTNNAC 1 cut(s) 310
Hpy99I CGWCG 3 cut(s) 17, 267, 290
HpyAV CCTTC 2 cut(s) 240, 466
Hsp92II CATG 2 cut(s) 278, 344
HspAI GCGC 2 cut(s) 61, 103
Kzo9I GATC 2 cut(s) 253, 456
LmnI GCTCC 6 cut(s) 6, 33, 58, 85, 138, 163
LpnPI CCDG 4 cut(s) 74, 152, 380, 461
Lsp1109I GCAGC 1 cut(s) 21
LweI GCATC 3 cut(s) 7, 58, 90
MaeI CTAG 1 cut(s) 580
MaeIII GTNAC 3 cut(s) 187, 277, 296
MalI GATC 2 cut(s) 255, 458
MboI GATC 2 cut(s) 253, 456
MboII GAAGA 1 cut(s) 367
MluCI AATT 2 cut(s) 230, 393
MmeI TCCRAC 2 cut(s) 434, 464
MnlI CCTC 6 cut(s) 16, 34, 48, 58, 117, 142
MseI TTAA 2 cut(s) 216, 293
MspCI CTTAAG 1 cut(s) 292
MspI CCGG 1 cut(s) 367
MvnI CGCG 3 cut(s) 47, 63, 105
NdeII GATC 2 cut(s) 253, 456
NlaIII CATG 2 cut(s) 278, 344
NlaIV GGNNCC 1 cut(s) 140
NmuCI GTSAC 3 cut(s) 187, 277, 296
NspV TTCGAA 1 cut(s) 530
PcsI WCGNNNNNNNCGW 1 cut(s) 205
PfeI GAWTC 5 cut(s) 113, 196, 337, 527, 574
PkrI GCNGC 4 cut(s) 11, 49, 73, 107
PsiI TTATAA 1 cut(s) 204
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 2 cut(s) 387, 481
SaqAI TTAA 2 cut(s) 216, 293
SatI GCNGC 4 cut(s) 10, 48, 72, 106
Sau3AI GATC 2 cut(s) 253, 456
Sau96I GGNCC 2 cut(s) 387, 481
SetI ASST 4 cut(s) 38, 153, 194, 477
SfaNI GCATC 3 cut(s) 7, 58, 90
SfuI TTCGAA 1 cut(s) 530
SinI GGWCC 2 cut(s) 387, 481
SmlI CTYRAG 1 cut(s) 292
SmoI CTYRAG 1 cut(s) 292
Sse9I AATT 2 cut(s) 230, 393
SspI AATATT 1 cut(s) 435
SspMI CTAG 1 cut(s) 580
TaqI TCGA 4 cut(s) 39, 238, 428, 530
TasI AATT 2 cut(s) 230, 393
TauI GCSGC 3 cut(s) 50, 74, 108
TfiI GAWTC 5 cut(s) 113, 196, 337, 527, 574
Tru1I TTAA 2 cut(s) 216, 293
Tru9I TTAA 2 cut(s) 216, 293
TscAI CASTG 1 cut(s) 455
TseFI GTSAC 3 cut(s) 187, 277, 296
TseI GCWGC 1 cut(s) 9
Tsp45I GTSAC 3 cut(s) 187, 277, 296
TspDTI ATGAA 3 cut(s) 310, 350, 508
TspGWI ACGGA 2 cut(s) 106, 369
TspRI CASTG 1 cut(s) 455
Vha464I CTTAAG 1 cut(s) 292
VpaK11BI GGWCC 2 cut(s) 387, 481
XapI RAATTY 1 cut(s) 393
XspI CTAG 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.