Rh3BG329400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0016984
Physical Location & Seq
Reverse (-)
34420601 .. 34421956
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG329400.1

Sequence Viewer

Length: 378 bp
ATGTCGATGACCACTGTCCGGGACTGGCCTTCTTCCGCTTATTTCATTCAAGGTTTGAGCGATGAAGGAGCCCTTCATGGTGATGGCCCGGCGCTGCTACTCAAGGAGGGAGTCCATGAAGCGGAGGCGGCCGGTGAGCTTGGGGTGGATGATGCCGTTGACGGAGCGGGAGATAAGGATGAAGATAAGGATGTGGTTTCATGGAATGCTATGGTTACTGGGTATTCTCAGATTGTTAGATTTGAGAATGCTATTGGTTTATTTGAAAACATAAGGGAGGAGAAGATTCAGTTAAATGTTGTCACTTGGAGTGTTATGATTGCAGGGTATGCGCAAAGGGAACATGGTTACCAAGCATGGATGTGTTTCGGCAAATGA

Protein Analysis

125

Amino Acids

13.71

Weight (kDa)

4.4

Isoelectric Point (pI)

15.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 63 - 111 4.9e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 333
AccBSI CCGCTC 1 cut(s) 167
AciI CCGC 4 cut(s) 36, 122, 128, 167
AcoI YGGCCR 1 cut(s) 129
AfiI CCNNNNNNNGG 2 cut(s) 18, 121
AgsI TTSAA 2 cut(s) 50, 266
AluBI AGCT 1 cut(s) 139
AluI AGCT 1 cut(s) 139
AoxI GGCC 3 cut(s) 26, 85, 129
ApeKI GCWGC 1 cut(s) 94
AspLEI GCGC 2 cut(s) 94, 334
AspS9I GGNCC 1 cut(s) 86
AsuC2I CCSGG 2 cut(s) 20, 89
AsuHPI GGTGA 2 cut(s) 92, 146
BanII GRGCYC 1 cut(s) 73
BbvI GCAGC 1 cut(s) 81
BccI CCATC 1 cut(s) 77
BceAI ACGGC 1 cut(s) 140
BcnI CCSGG 2 cut(s) 20, 89
BfoI RGCGCY 1 cut(s) 95
BisI GCNGC 2 cut(s) 95, 129
BlsI GCNGC 2 cut(s) 96, 130
Bme1390I CCNGG 2 cut(s) 20, 89
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 1 cut(s) 70
BmrFI CCNGG 2 cut(s) 20, 89
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 142
BmuI ACTGGG 1 cut(s) 228
BoxI GACNNNNGTC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 2 cut(s) 20, 89
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 121
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 2 cut(s) 29, 223
BseGI GGATG 4 cut(s) 154, 184, 196, 366
BseLI CCNNNNNNNGG 2 cut(s) 18, 121
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 2 cut(s) 29, 223
BseRI GAGGAG 1 cut(s) 293
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 1 cut(s) 81
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 28, 87, 131
BsiEI CGRYCG 1 cut(s) 132
BsiSI CCGG 3 cut(s) 19, 89, 132
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 2 cut(s) 18, 121
BsmFI GGGAC 1 cut(s) 35
BsmI GAATGC 2 cut(s) 211, 253
BsnI GGCC 3 cut(s) 28, 87, 131
Bsp1286I GDGCHC 1 cut(s) 73
BspACI CCGC 4 cut(s) 36, 122, 128, 167
BspANI GGCC 3 cut(s) 28, 87, 131
BspCNI CTCAG 1 cut(s) 241
BspLI GGNNCC 1 cut(s) 70
BsrBI CCGCTC 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 2 cut(s) 29, 223
BssAI RCCGGY 1 cut(s) 131
Bst4CI ACNGT 1 cut(s) 16
BstAPI GCANNNNNTGC 1 cut(s) 329
BstDEI CTNAG 1 cut(s) 228
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 4 cut(s) 154, 184, 196, 366
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 2 cut(s) 94, 334
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 128, 329
BstPAI GACNNNNGTC 1 cut(s) 14
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 2 cut(s) 18, 87
BstV1I GCAGC 1 cut(s) 81
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 3 cut(s) 28, 87, 131
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 4 cut(s) 154, 184, 196, 366
BtsIMutI CAGTG 1 cut(s) 12
CfoI GCGC 2 cut(s) 94, 334
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 86
CviAII CATG 5 cut(s) 77, 116, 201, 344, 357
CviJI RGCY 5 cut(s) 28, 71, 87, 131, 139
CviKI_1 RGCY 5 cut(s) 28, 71, 87, 131, 139
DdeI CTNAG 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 129
EagI CGGCCG 1 cut(s) 129
EclXI CGGCCG 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 73
Eco52I CGGCCG 1 cut(s) 129
Eco91I GGTNACC 1 cut(s) 347
EcoO65I GGTNACC 1 cut(s) 347
EcoT38I GRGCYC 1 cut(s) 73
FaeI CATG 5 cut(s) 80, 119, 204, 347, 360
FaiI YATR 9 cut(s) 78, 117, 202, 212, 272, 317, 330, 345, 358
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FaqI GGGAC 1 cut(s) 35
FatI CATG 5 cut(s) 76, 115, 200, 343, 356
FauI CCCGC 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 95, 129
FokI GGATG 4 cut(s) 161, 191, 203, 373
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 2 cut(s) 95, 129
FspI TGCGCA 1 cut(s) 333
GlaI GCGC 2 cut(s) 93, 333
GluI GCNGC 2 cut(s) 95, 129
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 3 cut(s) 28, 87, 131
HapII CCGG 3 cut(s) 19, 89, 132
HhaI GCGC 2 cut(s) 94, 334
Hin1II CATG 5 cut(s) 80, 119, 204, 347, 360
Hin6I GCGC 2 cut(s) 92, 332
HinP1I GCGC 2 cut(s) 92, 332
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 2 cut(s) 111, 286
HpaII CCGG 3 cut(s) 19, 89, 132
HphI GGTGA 2 cut(s) 92, 146
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 1 cut(s) 231
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 3 cut(s) 39, 59, 83
HpyCH4III ACNGT 1 cut(s) 16
HpyCH4V TGCA 1 cut(s) 323
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 329
HpyF3I CTNAG 1 cut(s) 228
Hsp92II CATG 5 cut(s) 80, 119, 204, 347, 360
HspAI GCGC 2 cut(s) 92, 332
LmnI GCTCC 2 cut(s) 68, 164
LpnPI CCDG 6 cut(s) 10, 32, 102, 145, 204, 309
Lsp1109I GCAGC 1 cut(s) 81
LweI GCATC 1 cut(s) 142
MaeIII GTNAC 3 cut(s) 214, 301, 347
MbiI CCGCTC 1 cut(s) 167
MboII GAAGA 3 cut(s) 24, 194, 295
MhlI GDGCHC 1 cut(s) 73
MlyI GAGTC 1 cut(s) 120
MnlI CCTC 3 cut(s) 100, 118, 271
MseI TTAA 1 cut(s) 293
MslI CAYNNNNRTG 2 cut(s) 81, 361
MspI CCGG 3 cut(s) 19, 89, 132
MspR9I CCNGG 2 cut(s) 20, 89
Mva1269I GAATGC 2 cut(s) 211, 253
MwoI GCNNNNNNNGC 2 cut(s) 128, 329
NciI CCSGG 2 cut(s) 20, 89
NlaIII CATG 5 cut(s) 80, 119, 204, 347, 360
NlaIV GGNNCC 1 cut(s) 70
NmuCI GTSAC 1 cut(s) 301
NsbI TGCGCA 1 cut(s) 333
PctI GAATGC 2 cut(s) 211, 253
PfeI GAWTC 1 cut(s) 286
PfoI TCCNGGA 1 cut(s) 18
PkrI GCNGC 2 cut(s) 96, 130
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
PshAI GACNNNNGTC 1 cut(s) 14
PspEI GGTNACC 1 cut(s) 347
PspN4I GGNNCC 1 cut(s) 70
PspPI GGNCC 1 cut(s) 86
RseI CAYNNNNRTG 2 cut(s) 81, 361
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 2 cut(s) 95, 129
Sau96I GGNCC 1 cut(s) 86
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 2 cut(s) 20, 89
SduI GDGCHC 1 cut(s) 73
SetI ASST 2 cut(s) 55, 141
SfaNI GCATC 1 cut(s) 142
SmiMI CAYNNNNRTG 2 cut(s) 81, 361
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
SsiI CCGC 4 cut(s) 36, 122, 128, 167
StyD4I CCNGG 2 cut(s) 18, 87
TaaI ACNGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 5
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 1 cut(s) 286
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 1 cut(s) 94
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 6 cut(s) 34, 65, 78, 132, 189, 195
TspGWI ACGGA 1 cut(s) 177
TspRI CASTG 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.