Rroxscaffold_7G00193780

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017436
Physical Location & Seq
Forward (+)
35318396 .. 35323560
5165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00193780.1

Sequence Viewer

Length: 594 bp
ATGGTAACTACCCTATCTTGCCGGATCGAGGGACGGTCCTCCGGTCTAGGTACATTGCCCTGGTCGTGGGCTTTGAGTATCTATAGCATTTGGGTGGGTGGATTGTTGGATCTGTCTAACCTGTGGACATTGCACGGCTGCGGTAGATTGGGTCTGAGGAGCGGCTACAGTGCACGGCTTCTCATCTGGCCAACGCTGCATGAGGATCTGCTGCAAGGTTCGGCGTTCGAGGGGTGTAGCAGGCCGAGTCTTCGGCGGTCTTTGCTCGATATGGTGTTGCACGGCTGGAAAAGCCAGCGGTTAGAGCTATTCGAACAGTGCGCCGCCGGAGGACAGGACGGCTGTGGTGGCAAAGAAGCAGCGACAGTGATGCTCCTCCTCTGTTGGCTTAGGGTTAGGCCTGGACTCAACCTTGGGCTGATGAGGCAGGGGACTCTTCCTGCTGGACAAGACTTGATTTGGGCTTGGAGCTACGGGTTTGGGCTTAGAATCAGTATTGAGCCAGATATGTTGAAAGCATGGGCTGATTCTGAAGGCATAAAGGTACTTGAGCATACTTGGGTTGGATCAACACAGCAAGAGTTGCTGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.79

Weight (kDa)

6.95

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 162
AciI CCGC 5 cut(s) 141, 162, 256, 298, 324
AclWI GGATC 4 cut(s) 32, 117, 213, 574
AcoI YGGCCR 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 552
AfaI GTAC 2 cut(s) 52, 546
AfiI CCNNNNNNNGG 2 cut(s) 28, 66
AgsI TTSAA 1 cut(s) 514
AjnI CCWGG 2 cut(s) 59, 400
AluBI AGCT 2 cut(s) 307, 471
AluI AGCT 2 cut(s) 307, 471
Alw21I GWGCWC 1 cut(s) 175
Alw44I GTGCAC 1 cut(s) 171
AlwI GGATC 4 cut(s) 32, 117, 213, 574
AoxI GGCC 3 cut(s) 188, 242, 398
ApaLI GTGCAC 1 cut(s) 171
ApeKI GCWGC 5 cut(s) 138, 196, 211, 359, 586
AspLEI GCGC 1 cut(s) 323
AspS9I GGNCC 1 cut(s) 36
AsuII TTCGAA 1 cut(s) 312
AvaII GGWCC 1 cut(s) 36
BaeGI GKGCMC 1 cut(s) 175
BalI TGGCCA 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 5 cut(s) 125, 183, 198, 371, 573
BceAI ACGGC 4 cut(s) 151, 191, 298, 355
BcgI CGANNNNNNTGC 2 cut(s) 352, 386
BciT130I CCWGG 2 cut(s) 61, 402
BfaI CTAG 1 cut(s) 47
BfmI CTRYAG 2 cut(s) 82, 166
BisI GCNGC 7 cut(s) 139, 163, 197, 212, 324, 360, 587
BlsI GCNGC 7 cut(s) 140, 164, 198, 213, 325, 361, 588
Bme1390I CCNGG 2 cut(s) 61, 402
Bme18I GGWCC 1 cut(s) 36
BmgT120I GGNCC 1 cut(s) 36
BmrFI CCNGG 2 cut(s) 61, 402
BmsI GCATC 1 cut(s) 360
BpiI GAAGAC 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 389
Bpu14I TTCGAA 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 569
BsaJI CCNNGG 2 cut(s) 59, 412
BsaWI WCCGGW 1 cut(s) 41
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 66
Bse3DI GCAATG 2 cut(s) 53, 128
BseBI CCWGG 2 cut(s) 61, 402
BseDI CCNNGG 2 cut(s) 59, 412
BseLI CCNNNNNNNGG 2 cut(s) 28, 66
BseMI GCAATG 2 cut(s) 53, 128
BseMII CTCAG 1 cut(s) 146
BseRI GAGGAG 3 cut(s) 172, 365, 368
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 5 cut(s) 125, 183, 198, 371, 573
BshFI GGCC 3 cut(s) 190, 244, 400
BsiHKAI GWGCWC 1 cut(s) 175
BsiSI CCGG 3 cut(s) 22, 42, 327
BslFI GGGAC 2 cut(s) 45, 445
BslI CCNNNNNNNGG 2 cut(s) 28, 66
BsmFI GGGAC 2 cut(s) 45, 445
BsnI GGCC 3 cut(s) 190, 244, 400
Bsp119I TTCGAA 1 cut(s) 312
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 4 cut(s) 24, 109, 205, 566
BspACI CCGC 5 cut(s) 141, 162, 256, 298, 324
BspANI GGCC 3 cut(s) 190, 244, 400
BspCNI CTCAG 1 cut(s) 147
BspPI GGATC 4 cut(s) 32, 117, 213, 574
BspT104I TTCGAA 1 cut(s) 312
BsrBI CCGCTC 1 cut(s) 162
BsrDI GCAATG 2 cut(s) 53, 128
BssECI CCNNGG 2 cut(s) 59, 412
BssMI GATC 4 cut(s) 24, 109, 205, 566
BssT1I CCWWGG 1 cut(s) 412
Bst2UI CCWGG 2 cut(s) 61, 402
Bst4CI ACNGT 4 cut(s) 36, 170, 318, 367
Bst6I CTCTTC 1 cut(s) 441
BstAPI GCANNNNNTGC 1 cut(s) 583
BstBI TTCGAA 1 cut(s) 312
BstC8I GCNNGC 2 cut(s) 242, 296
BstDEI CTNAG 3 cut(s) 155, 389, 485
BstHHI GCGC 1 cut(s) 323
BstKTI GATC 4 cut(s) 27, 112, 208, 569
BstMBI GATC 4 cut(s) 24, 109, 205, 566
BstMWI GCNNNNNNNGC 7 cut(s) 196, 262, 291, 304, 348, 424, 583
BstNI CCWGG 2 cut(s) 61, 402
BstSCI CCNGG 2 cut(s) 59, 400
BstSFI CTRYAG 2 cut(s) 82, 166
BstSLI GKGCMC 1 cut(s) 175
BstV1I GCAGC 5 cut(s) 125, 183, 198, 371, 573
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 2 cut(s) 109, 205
BstYI RGATCY 2 cut(s) 109, 205
BsuRI GGCC 3 cut(s) 190, 244, 400
BtsIMutI CAGTG 3 cut(s) 175, 323, 372
Cac8I GCNNGC 2 cut(s) 242, 296
CfoI GCGC 1 cut(s) 323
Cfr13I GGNCC 1 cut(s) 36
Csp6I GTAC 2 cut(s) 51, 545
CviAII CATG 2 cut(s) 200, 519
CviQI GTAC 2 cut(s) 51, 545
DdeI CTNAG 3 cut(s) 155, 389, 485
DpnI GATC 4 cut(s) 26, 111, 207, 568
DpnII GATC 4 cut(s) 24, 109, 205, 566
EaeI YGGCCR 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 412
Eco147I AGGCCT 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 552
EcoRII CCWGG 2 cut(s) 59, 400
EcoT14I CCWWGG 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 412
FaeI CATG 2 cut(s) 203, 522
FaiI YATR 7 cut(s) 84, 201, 272, 509, 520, 539, 555
FaqI GGGAC 2 cut(s) 45, 445
FatI CATG 2 cut(s) 199, 518
Fnu4HI GCNGC 7 cut(s) 139, 163, 197, 212, 324, 360, 587
Fsp4HI GCNGC 7 cut(s) 139, 163, 197, 212, 324, 360, 587
FspBI CTAG 1 cut(s) 47
GlaI GCGC 1 cut(s) 322
GluI GCNGC 7 cut(s) 139, 163, 197, 212, 324, 360, 587
HaeIII GGCC 3 cut(s) 190, 244, 400
HapII CCGG 3 cut(s) 22, 42, 327
HhaI GCGC 1 cut(s) 323
Hin1II CATG 2 cut(s) 203, 522
Hin6I GCGC 1 cut(s) 321
HinP1I GCGC 1 cut(s) 321
HinfI GANTC 5 cut(s) 247, 405, 433, 489, 527
HpaII CCGG 3 cut(s) 22, 42, 327
Hpy166II GTNNAC 2 cut(s) 126, 173
Hpy188I TCNGA 2 cut(s) 156, 532
Hpy8I GTNNAC 2 cut(s) 126, 173
HpyAV CCTTC 1 cut(s) 527
HpyCH4III ACNGT 4 cut(s) 36, 170, 318, 367
HpyCH4V TGCA 6 cut(s) 133, 173, 199, 214, 280, 589
HpyF10VI GCNNNNNNNGC 7 cut(s) 196, 262, 291, 304, 348, 424, 583
HpyF3I CTNAG 3 cut(s) 155, 389, 485
Hsp92II CATG 2 cut(s) 203, 522
HspAI GCGC 1 cut(s) 321
Kzo9I GATC 4 cut(s) 24, 109, 205, 566
LmnI GCTCC 3 cut(s) 159, 378, 468
Lsp1109I GCAGC 5 cut(s) 125, 183, 198, 371, 573
LweI GCATC 1 cut(s) 360
MaeI CTAG 1 cut(s) 47
MaeIII GTNAC 1 cut(s) 4
MalI GATC 4 cut(s) 26, 111, 207, 568
MbiI CCGCTC 1 cut(s) 162
MboI GATC 4 cut(s) 24, 109, 205, 566
MboII GAAGA 2 cut(s) 242, 428
MflI RGATCY 2 cut(s) 109, 205
MhlI GDGCHC 1 cut(s) 175
MlsI TGGCCA 1 cut(s) 190
MluNI TGGCCA 1 cut(s) 190
MlyI GAGTC 3 cut(s) 256, 399, 427
MmeI TCCRAC 2 cut(s) 87, 544
MnlI CCTC 9 cut(s) 22, 49, 150, 196, 223, 323, 386, 389, 417
Mox20I TGGCCA 1 cut(s) 190
MscI TGGCCA 1 cut(s) 190
MslI CAYNNNNRTG 1 cut(s) 92
Msp20I TGGCCA 1 cut(s) 190
MspA1I CMGCKG 1 cut(s) 298
MspI CCGG 3 cut(s) 22, 42, 327
MspR9I CCNGG 2 cut(s) 61, 402
MvaI CCWGG 2 cut(s) 61, 402
MwoI GCNNNNNNNGC 7 cut(s) 196, 262, 291, 304, 348, 424, 583
NdeII GATC 4 cut(s) 24, 109, 205, 566
NlaIII CATG 2 cut(s) 203, 522
NmeAIII GCCGAG 1 cut(s) 270
NspV TTCGAA 1 cut(s) 312
PceI AGGCCT 1 cut(s) 400
PfeI GAWTC 2 cut(s) 489, 527
PkrI GCNGC 7 cut(s) 140, 164, 198, 213, 325, 361, 588
PleI GAGTC 3 cut(s) 255, 399, 427
PpsI GAGTC 3 cut(s) 255, 399, 427
Psp6I CCWGG 2 cut(s) 59, 400
PspGI CCWGG 2 cut(s) 59, 400
PspPI GGNCC 1 cut(s) 36
PsuI RGATCY 2 cut(s) 109, 205
RsaI GTAC 2 cut(s) 52, 546
RsaNI GTAC 2 cut(s) 51, 545
RseI CAYNNNNRTG 1 cut(s) 92
SatI GCNGC 7 cut(s) 139, 163, 197, 212, 324, 360, 587
Sau3AI GATC 4 cut(s) 24, 109, 205, 566
Sau96I GGNCC 1 cut(s) 36
SchI GAGTC 3 cut(s) 256, 399, 427
ScrFI CCNGG 2 cut(s) 61, 402
SduI GDGCHC 1 cut(s) 175
SetI ASST 7 cut(s) 52, 123, 220, 309, 414, 473, 546
SfaNI GCATC 1 cut(s) 360
SfcI CTRYAG 2 cut(s) 82, 166
SfuI TTCGAA 1 cut(s) 312
SinI GGWCC 1 cut(s) 36
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
SseBI AGGCCT 1 cut(s) 400
SsiI CCGC 5 cut(s) 141, 162, 256, 298, 324
SspMI CTAG 1 cut(s) 47
StuI AGGCCT 1 cut(s) 400
StyD4I CCNGG 2 cut(s) 59, 400
StyI CCWWGG 1 cut(s) 412
TaaI ACNGT 4 cut(s) 36, 170, 318, 367
TaqI TCGA 4 cut(s) 27, 228, 267, 312
TauI GCSGC 2 cut(s) 165, 326
TfiI GAWTC 2 cut(s) 489, 527
TscAI CASTG 3 cut(s) 175, 323, 372
TseI GCWGC 5 cut(s) 138, 196, 211, 359, 586
TspRI CASTG 3 cut(s) 175, 323, 372
VneI GTGCAC 1 cut(s) 171
VpaK11BI GGWCC 1 cut(s) 36
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.