Rroxscaffold_7G00205420

50S ribosome-binding GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005540
Physical Location & Seq
Reverse (-)
54991827 .. 54999601
7775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00205420.1

Sequence Viewer

Length: 1341 bp
ATGCAACCTACTTCTTTCAAGTTAAGTTGGCCTTCTTTGGACCATATACCACCCGGGGAAGTTAGAGACCAGACTGGAATCCTTAACGTCCAAACAACCCAAAGATCCTACACGTACAATTCAGAGGGGGACATTGAGGGTAGCTATGAGGAAAGTGATGGGGAAGAAAGTGAATTGGAAATGGGGGATGATGAGTTTGACTGGGATTCAGATGACTTGGAATCTAAGTTGTTGGGGGAGGAAGAAGAGAATGATTTGGATTTGGATGGTTTTGCCCCTGCAGGGGTTGGGTATGGTAACATTAAGGAGGAGACTATAGAGAAAATGAAGAGGAAGAAGATTCCAAAAGCCGAAAAGAAGAGAATGGCTAGAGAGGCTCAGAAAGAGAAAGATGAGGTTACTGTGTGCGCCAGATGCCATTCGTTGAGAAATTATGGGCAGGTGAAAAACCAAGCAGCCGAAAACTTGATGCCCGATTTTGATTTCGATAGGTTGATTGCAACCCGGTTGATTAAACCTGCTACGAATGCCAATCCTGTTGTGGTTATGGTTGTTGATTGTGTTGATTTTGATGGCTCATTCCCCAAACGGGCAGCAAAGTCATTGTTTCAGGCATTGAGAGGAACCGAGAATGATCCCAAGTTTCGGAAAAAAAAAATGCCAAAGCTTGTTCTTGTGGCCACAAAGGTTGATCTCCTCCCATCACAAGTTTCACCTTCTAGATTAGATAGATGGGTACGCCATCGTGCTAAGGCTGGAGGGGGACCTAAACTGAGTGGAGTGTATTTGGTTAGTGCTCATAAGGATGTGGGTGTCAGAAATTTGTTGTCCTTTCTCAAAGAATTGGCAGGTCCTCGAGGGAATGTGTGGGTTGTTGGAGCTCAAAATGCAGGCAAGTCTACTTTAATAAATGCGCTGGCAAAGAAAGAAGGGGCAAAGGTTACAAAGCTAACTGAAGCTCCAGTTCCTGGCACAACATTAGGGATCCTGAGAGTTGGAGGGATTTTGTCAGCCAAGGCAAAGTTGTTTGACACTCCAGGACTTTTACATCCATATTTGGTCTCCATGAGATTGAATAGGGATGAACAGAAAATGGCGTCATGTGACATTTTTTATCGGAAAAATTCACCGGCAAAGCAAAGTGACACTACTACAAATCTAAGTAATAGGCACACCCAGTTATACGTGCCCTTGACCCAGCTTATACGTGCCTTTGATCACAATACCCACCAATCATATGTAGCTATAGGCAATAGCAACGCTGCAACCACGAATTCAAGGTCCGTGTCCTTCATCGCCGTGGCTTTGGCAGGGATAGCCACGCTTCCGTTTTATTCGTGA

Protein Analysis

446

Amino Acids

49.26

Weight (kDa)

8.62

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 289 - 370 1.1e-10 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 430
AbsI CCTCGAGG 1 cut(s) 855
Acc36I ACCTGC 3 cut(s) 430, 526, 839
AccB7I CCANNNNNTGG 1 cut(s) 968
AccI GTMKAC 1 cut(s) 899
AclWI GGATC 4 cut(s) 99, 629, 979, 992
AcoI YGGCCR 1 cut(s) 678
AcsI RAATTY 3 cut(s) 820, 1123, 1273
AcuI CTGAAG 1 cut(s) 975
AcyI GRCGYC 1 cut(s) 1097
AfaI GTAC 2 cut(s) 116, 738
AfiI CCNNNNNNNGG 5 cut(s) 282, 283, 589, 645, 968
AflIII ACRYGT 1 cut(s) 111
AgsI TTSAA 3 cut(s) 19, 1075, 1278
AjnI CCWGG 2 cut(s) 967, 1036
AluBI AGCT 7 cut(s) 144, 667, 881, 949, 959, 1201, 1244
AluI AGCT 7 cut(s) 144, 667, 881, 949, 959, 1201, 1244
Alw21I GWGCWC 2 cut(s) 799, 883
Alw26I GTCTC 3 cut(s) 60, 305, 1066
AlwI GGATC 4 cut(s) 99, 629, 979, 992
AlwNI CAGNNNCTG 1 cut(s) 968
Ama87I CYCGRG 2 cut(s) 53, 855
AoxI GGCC 2 cut(s) 29, 678
ApeKI GCWGC 3 cut(s) 455, 593, 1262
ApoI RAATTY 3 cut(s) 820, 1123, 1273
ArsI GACNNNNNNTTYG 2 cut(s) 1265, 1297
AspLEI GCGC 2 cut(s) 410, 916
AspS9I GGNCC 4 cut(s) 40, 764, 851, 1281
AsuC2I CCSGG 3 cut(s) 54, 55, 505
AsuHPI GGTGA 3 cut(s) 454, 705, 1119
AvaI CYCGRG 2 cut(s) 53, 855
AvaII GGWCC 4 cut(s) 40, 764, 851, 1281
BaeGI GKGCMC 1 cut(s) 1191
BalI TGGCCA 1 cut(s) 680
BamHI GGATCC 1 cut(s) 984
BanII GRGCYC 1 cut(s) 883
Bbv12I GWGCWC 2 cut(s) 799, 883
BbvI GCAGC 3 cut(s) 467, 605, 1249
BccI CCATC 6 cut(s) 152, 260, 566, 709, 726, 750
BceAI ACGGC 1 cut(s) 1283
BciT130I CCWGG 2 cut(s) 969, 1038
BclI TGATCA 1 cut(s) 1216
BcnI CCSGG 3 cut(s) 54, 55, 505
BcoDI GTCTC 3 cut(s) 60, 305, 1066
BfaI CTAG 2 cut(s) 369, 720
BfmI CTRYAG 3 cut(s) 279, 315, 1245
BfuAI ACCTGC 3 cut(s) 430, 526, 839
BisI GCNGC 3 cut(s) 456, 594, 1263
BlsI GCNGC 3 cut(s) 457, 595, 1264
Bme1390I CCNGG 5 cut(s) 54, 55, 505, 969, 1038
Bme18I GGWCC 4 cut(s) 40, 764, 851, 1281
BmeT110I CYCGRG 2 cut(s) 53, 855
BmgT120I GGNCC 4 cut(s) 40, 764, 851, 1281
BmiI GGNNCC 3 cut(s) 625, 765, 986
BmrFI CCNGG 5 cut(s) 54, 55, 505, 969, 1038
BmrI ACTGGG 2 cut(s) 211, 1171
BmsI GCATC 2 cut(s) 404, 459
BmuI ACTGGG 2 cut(s) 211, 1171
BpmI CTGGAG 3 cut(s) 777, 945, 1020
Bpu10I CCTNAGC 1 cut(s) 750
BpuMI CCSGG 3 cut(s) 54, 55, 505
BsaAI YACGTR 3 cut(s) 114, 1186, 1208
BsaHI GRCGYC 1 cut(s) 1097
BsaI GGTCTC 2 cut(s) 60, 1066
BsaJI CCNNGG 4 cut(s) 53, 54, 1014, 1299
BsaXI ACNNNNNCTCC 2 cut(s) 943, 973
Bsc4I CCNNNNNNNGG 5 cut(s) 282, 283, 589, 645, 968
Bse118I RCCGGY 1 cut(s) 1129
Bse1I ACTGG 4 cut(s) 79, 206, 962, 1177
BseBI CCWGG 2 cut(s) 969, 1038
BseDI CCNNGG 4 cut(s) 53, 54, 1014, 1299
BseGI GGATG 5 cut(s) 193, 271, 811, 1048, 1087
BseLI CCNNNNNNNGG 5 cut(s) 282, 283, 589, 645, 968
BseMII CTCAG 3 cut(s) 392, 764, 980
BseNI ACTGG 4 cut(s) 79, 206, 962, 1177
BseRI GAGGAG 2 cut(s) 323, 686
BseSI GKGCMC 1 cut(s) 1191
BseXI GCAGC 3 cut(s) 467, 605, 1249
BseYI CCCAGC 1 cut(s) 1197
BshFI GGCC 2 cut(s) 31, 680
BsiHKAI GWGCWC 2 cut(s) 799, 883
BsiHKCI CYCGRG 2 cut(s) 53, 855
BsiSI CCGG 3 cut(s) 54, 505, 1130
BslFI GGGAC 2 cut(s) 143, 777
BslI CCNNNNNNNGG 5 cut(s) 282, 283, 589, 645, 968
BsmAI GTCTC 3 cut(s) 60, 305, 1066
BsmFI GGGAC 2 cut(s) 143, 777
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 2 cut(s) 31, 680
Bso31I GGTCTC 2 cut(s) 60, 1066
BsoBI CYCGRG 2 cut(s) 53, 855
Bsp1286I GDGCHC 3 cut(s) 799, 883, 1191
Bsp143I GATC 5 cut(s) 104, 634, 691, 984, 1216
BspANI GGCC 2 cut(s) 31, 680
BspCNI CTCAG 3 cut(s) 391, 765, 981
BspLI GGNNCC 3 cut(s) 625, 765, 986
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 3 cut(s) 430, 526, 839
BspPI GGATC 4 cut(s) 99, 629, 979, 992
BspTNI GGTCTC 2 cut(s) 60, 1066
BsrFI RCCGGY 1 cut(s) 1129
BsrI ACTGG 4 cut(s) 79, 206, 962, 1177
BssAI RCCGGY 1 cut(s) 1129
BssECI CCNNGG 4 cut(s) 53, 54, 1014, 1299
BssMI GATC 5 cut(s) 104, 634, 691, 984, 1216
BssNI GRCGYC 1 cut(s) 1097
BssT1I CCWWGG 1 cut(s) 1014
Bst2UI CCWGG 2 cut(s) 969, 1038
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 3 cut(s) 240, 323, 353
BstACI GRCGYC 1 cut(s) 1097
BstBAI YACGTR 3 cut(s) 114, 1186, 1208
BstC8I GCNNGC 2 cut(s) 892, 918
BstDEI CTNAG 6 cut(s) 225, 378, 750, 773, 989, 1160
BstDSI CCRYGG 1 cut(s) 1299
BstF5I GGATG 5 cut(s) 193, 271, 811, 1048, 1087
BstHHI GCGC 2 cut(s) 410, 916
BstKTI GATC 5 cut(s) 107, 637, 694, 987, 1219
BstMAI GTCTC 3 cut(s) 60, 305, 1066
BstMBI GATC 5 cut(s) 104, 634, 691, 984, 1216
BstMWI GCNNNNNNNGC 5 cut(s) 374, 414, 527, 887, 1316
BstNI CCWGG 2 cut(s) 969, 1038
BstSCI CCNGG 5 cut(s) 52, 53, 503, 967, 1036
BstSFI CTRYAG 3 cut(s) 279, 315, 1245
BstSLI GKGCMC 1 cut(s) 1191
BstV1I GCAGC 3 cut(s) 467, 605, 1249
BstX2I RGATCY 2 cut(s) 104, 984
BstYI RGATCY 2 cut(s) 104, 984
BsuRI GGCC 2 cut(s) 31, 680
BtgI CCRYGG 1 cut(s) 1299
BtgZI GCGATG 1 cut(s) 1279
BtsCI GGATG 5 cut(s) 193, 271, 811, 1048, 1087
BveI ACCTGC 3 cut(s) 430, 526, 839
Cac8I GCNNGC 2 cut(s) 892, 918
CaiI CAGNNNCTG 1 cut(s) 968
CfoI GCGC 2 cut(s) 410, 916
Cfr10I RCCGGY 1 cut(s) 1129
Cfr13I GGNCC 4 cut(s) 40, 764, 851, 1281
Cfr9I CCCGGG 1 cut(s) 53
CseI GACGC 1 cut(s) 1086
Csp6I GTAC 2 cut(s) 115, 737
CspCI CAANNNNNGTGG 2 cut(s) 670, 705
CviAII CATG 2 cut(s) 1066, 1101
CviQI GTAC 2 cut(s) 115, 737
DdeI CTNAG 6 cut(s) 225, 378, 750, 773, 989, 1160
DpnI GATC 5 cut(s) 106, 636, 693, 986, 1218
DpnII GATC 5 cut(s) 104, 634, 691, 984, 1216
EaeI YGGCCR 1 cut(s) 678
Eam1104I CTCTTC 3 cut(s) 240, 323, 353
EarI CTCTTC 3 cut(s) 240, 323, 353
Ecl136II GAGCTC 1 cut(s) 881
Eco130I CCWWGG 1 cut(s) 1014
Eco24I GRGCYC 1 cut(s) 883
Eco31I GGTCTC 2 cut(s) 60, 1066
Eco47I GGWCC 4 cut(s) 40, 764, 851, 1281
Eco53kI GAGCTC 1 cut(s) 881
Eco57I CTGAAG 1 cut(s) 975
Eco88I CYCGRG 2 cut(s) 53, 855
EcoICRI GAGCTC 1 cut(s) 881
EcoO109I RGGNCCY 2 cut(s) 764, 851
EcoRI GAATTC 1 cut(s) 1273
EcoRII CCWGG 2 cut(s) 967, 1036
EcoT14I CCWWGG 1 cut(s) 1014
EcoT38I GRGCYC 1 cut(s) 883
ErhI CCWWGG 1 cut(s) 1014
FaeI CATG 2 cut(s) 1069, 1104
FalI AAGNNNNNCTT 2 cut(s) 16, 48
FaqI GGGAC 2 cut(s) 143, 777
FatI CATG 2 cut(s) 1065, 1100
FauNDI CATATG 1 cut(s) 1237
FbaI TGATCA 1 cut(s) 1216
FblI GTMKAC 1 cut(s) 899
Fnu4HI GCNGC 3 cut(s) 456, 594, 1263
FokI GGATG 5 cut(s) 200, 278, 818, 1035, 1094
FriOI GRGCYC 1 cut(s) 883
Fsp4HI GCNGC 3 cut(s) 456, 594, 1263
FspBI CTAG 2 cut(s) 369, 720
GlaI GCGC 2 cut(s) 409, 915
GluI GCNGC 3 cut(s) 456, 594, 1263
GsaI CCCAGC 1 cut(s) 1201
GsuI CTGGAG 3 cut(s) 777, 945, 1020
HaeIII GGCC 2 cut(s) 31, 680
HapII CCGG 3 cut(s) 54, 505, 1130
HgaI GACGC 1 cut(s) 1086
HhaI GCGC 2 cut(s) 410, 916
Hin1I GRCGYC 1 cut(s) 1097
Hin1II CATG 2 cut(s) 1069, 1104
Hin6I GCGC 2 cut(s) 408, 914
HinP1I GCGC 2 cut(s) 408, 914
HindIII AAGCTT 1 cut(s) 665
HinfI GANTC 4 cut(s) 78, 206, 221, 340
HpaII CCGG 3 cut(s) 54, 505, 1130
HphI GGTGA 3 cut(s) 454, 705, 1119
Hpy166II GTNNAC 1 cut(s) 900
Hpy188I TCNGA 6 cut(s) 124, 211, 381, 648, 818, 1119
Hpy188III TCNNGA 3 cut(s) 720, 988, 1338
Hpy8I GTNNAC 1 cut(s) 900
HpyAV CCTTC 4 cut(s) 42, 726, 923, 1300
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4IV ACGT 4 cut(s) 87, 113, 1185, 1207
HpyCH4V TGCA 5 cut(s) 4, 281, 500, 890, 1265
HpyF10VI GCNNNNNNNGC 5 cut(s) 374, 414, 527, 887, 1316
HpyF3I CTNAG 6 cut(s) 225, 378, 750, 773, 989, 1160
HpySE526I ACGT 4 cut(s) 87, 113, 1185, 1207
Hsp92I GRCGYC 1 cut(s) 1097
Hsp92II CATG 2 cut(s) 1069, 1104
HspAI GCGC 2 cut(s) 408, 914
Ksp22I TGATCA 1 cut(s) 1216
Kzo9I GATC 5 cut(s) 104, 634, 691, 984, 1216
LmnI GCTCC 2 cut(s) 878, 964
Lsp1109I GCAGC 3 cut(s) 467, 605, 1249
LweI GCATC 2 cut(s) 404, 459
MaeI CTAG 2 cut(s) 369, 720
MaeII ACGT 4 cut(s) 87, 113, 1185, 1207
MaeIII GTNAC 5 cut(s) 296, 397, 940, 1103, 1142
MalI GATC 5 cut(s) 106, 636, 693, 986, 1218
MboI GATC 5 cut(s) 104, 634, 691, 984, 1216
MboII GAAGA 7 cut(s) 176, 254, 257, 340, 346, 349, 370
MflI RGATCY 2 cut(s) 104, 984
MhlI GDGCHC 3 cut(s) 799, 883, 1191
MlsI TGGCCA 1 cut(s) 680
MluCI AATT 7 cut(s) 118, 173, 430, 820, 842, 1123, 1273
MluNI TGGCCA 1 cut(s) 680
MmeI TCCRAC 2 cut(s) 856, 976
Mox20I TGGCCA 1 cut(s) 680
MscI TGGCCA 1 cut(s) 680
MseI TTAA 5 cut(s) 23, 84, 303, 513, 905
MslI CAYNNNNRTG 2 cut(s) 804, 1298
Msp20I TGGCCA 1 cut(s) 680
MspI CCGG 3 cut(s) 54, 505, 1130
MspR9I CCNGG 5 cut(s) 54, 55, 505, 969, 1038
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 2 cut(s) 969, 1038
MwoI GCNNNNNNNGC 5 cut(s) 374, 414, 527, 887, 1316
NciI CCSGG 3 cut(s) 54, 55, 505
NdeI CATATG 1 cut(s) 1237
NdeII GATC 5 cut(s) 104, 634, 691, 984, 1216
NlaIII CATG 2 cut(s) 1069, 1104
NlaIV GGNNCC 3 cut(s) 625, 765, 986
NmuCI GTSAC 2 cut(s) 1103, 1142
PaeR7I CTCGAG 1 cut(s) 855
PaqCI CACCTGC 1 cut(s) 430
PctI GAATGC 1 cut(s) 532
PfeI GAWTC 4 cut(s) 78, 206, 221, 340
PflMI CCANNNNNTGG 1 cut(s) 968
PfoI TCCNGGA 1 cut(s) 1036
PkrI GCNGC 3 cut(s) 457, 595, 1264
Ppu21I YACGTR 3 cut(s) 114, 1186, 1208
PpuMI RGGWCCY 2 cut(s) 764, 851
Psp124BI GAGCTC 1 cut(s) 883
Psp5II RGGWCCY 2 cut(s) 764, 851
Psp6I CCWGG 2 cut(s) 967, 1036
PspFI CCCAGC 1 cut(s) 1197
PspGI CCWGG 2 cut(s) 967, 1036
PspN4I GGNNCC 3 cut(s) 625, 765, 986
PspPI GGNCC 4 cut(s) 40, 764, 851, 1281
PspPPI RGGWCCY 2 cut(s) 764, 851
PspXI VCTCGAGB 1 cut(s) 855
PstI CTGCAG 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 968
PsuI RGATCY 2 cut(s) 104, 984
RsaI GTAC 2 cut(s) 116, 738
RsaNI GTAC 2 cut(s) 115, 737
RseI CAYNNNNRTG 2 cut(s) 804, 1298
SacI GAGCTC 1 cut(s) 883
SaqAI TTAA 5 cut(s) 23, 84, 303, 513, 905
SatI GCNGC 3 cut(s) 456, 594, 1263
Sau3AI GATC 5 cut(s) 104, 634, 691, 984, 1216
Sau96I GGNCC 4 cut(s) 40, 764, 851, 1281
SbfI CCTGCAGG 1 cut(s) 283
ScrFI CCNGG 5 cut(s) 54, 55, 505, 969, 1038
SdaI CCTGCAGG 1 cut(s) 283
SduI GDGCHC 3 cut(s) 799, 883, 1191
SfaNI GCATC 2 cut(s) 404, 459
SfcI CTRYAG 3 cut(s) 279, 315, 1245
Sfr274I CTCGAG 1 cut(s) 855
SinI GGWCC 4 cut(s) 40, 764, 851, 1281
SlaI CTCGAG 1 cut(s) 855
SmaI CCCGGG 1 cut(s) 55
SmiMI CAYNNNNRTG 2 cut(s) 804, 1298
SmlI CTYRAG 1 cut(s) 855
SmoI CTYRAG 1 cut(s) 855
Sse8387I CCTGCAGG 1 cut(s) 283
Sse9I AATT 7 cut(s) 118, 173, 430, 820, 842, 1123, 1273
SspMI CTAG 2 cut(s) 369, 720
SstI GAGCTC 1 cut(s) 883
StyD4I CCNGG 5 cut(s) 52, 53, 503, 967, 1036
StyI CCWWGG 1 cut(s) 1014
TaaI ACNGT 1 cut(s) 403
TaiI ACGT 4 cut(s) 90, 116, 1188, 1210
TaqI TCGA 2 cut(s) 486, 856
TasI AATT 7 cut(s) 118, 173, 430, 820, 842, 1123, 1273
TfiI GAWTC 4 cut(s) 78, 206, 221, 340
Tru1I TTAA 5 cut(s) 23, 84, 303, 513, 905
Tru9I TTAA 5 cut(s) 23, 84, 303, 513, 905
TseFI GTSAC 2 cut(s) 1103, 1142
TseI GCWGC 3 cut(s) 455, 593, 1262
Tsp45I GTSAC 2 cut(s) 1103, 1142
TspDTI ATGAA 3 cut(s) 341, 1098, 1282
TspGWI ACGGA 2 cut(s) 1273, 1317
TspMI CCCGGG 1 cut(s) 53
Van91I CCANNNNNTGG 1 cut(s) 968
VpaK11BI GGWCC 4 cut(s) 40, 764, 851, 1281
XapI RAATTY 3 cut(s) 820, 1123, 1273
XbaI TCTAGA 1 cut(s) 719
XcmI CCANNNNNNNNNTGG 1 cut(s) 538
XhoI CTCGAG 1 cut(s) 855
XmaI CCCGGG 1 cut(s) 53
XmiI GTMKAC 1 cut(s) 899
XspI CTAG 2 cut(s) 369, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.