Rorug03G0212400

50S ribosome-binding GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005540
Physical Location & Seq
Reverse (-)
19109445 .. 19110128
684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0212400.1

Sequence Viewer

Length: 684 bp
ATGTCGGAGAAACAAGGCTTGAATGGAGCCTACTACGGCCCATCCCTCCCTCCCAAGAGGGAGTCCTACCAAAGCGTCGGCCACGGCGGAGGTCCCCTCGGCTGCTGCTGCAGCTGTATCTTCGGCCTTGTCTTCAAGCTCATCTTCACCGCCATCGTCTTCATGGGCCTCGCCATCTTTGTCTTCTGGATCATAGTCAAACCTCACCGTATCAAGTTCCACGTCACTGACGCCAACCTCACCCAGTTCAACTTCTCCTCAGACAGCAACCTCCACTACAACCTCGCCCTCAACCTCACCATCCGAAACCCTAACAAGAAGTTCGGCATCTACTACGACCGCATCGAAGCCAGAGCTAACTATGAGGGCCAGAGGTTCAGCACGATCACTCTCACCCCGTTTTACCAAGGACACAAAACCACCAACGTTTTGAACCCCGTGTTTAATGGACAGCAGTTGCTTGTTGGGTCCAATTTGCAGTCGGAGTATCAAAAGCAGACAAGTGCTGGGGTTTACGAAATTGAGATGAAGCTTTACATGGGGATTCGGTTCAAGTTCGGTCGAATCAAGACCGGAAAGTTCAAGCCCAGAGTTGAATGTGACTTGAAGGTTCCTTTCAGTACGAATGAGAATTTTGCAACTGCCTTTGAGACCAAGAGTTGCGAAGTTGATTATTTGAATTAG

Protein Analysis

227

Amino Acids

25.77

Weight (kDa)

9.24

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 99 - 193 1.9e-09 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 87, 150, 340
AclI AACGTT 1 cut(s) 426
AclWI GGATC 1 cut(s) 197
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 1 cut(s) 631
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 1 cut(s) 622
AgsI TTSAA 9 cut(s) 22, 136, 250, 433, 553, 583, 596, 607, 679
AjiI CACGTC 1 cut(s) 223
AluBI AGCT 4 cut(s) 114, 139, 356, 532
AluI AGCT 4 cut(s) 114, 139, 356, 532
Alw26I GTCTC 1 cut(s) 644
AlwI GGATC 1 cut(s) 197
AoxI GGCC 5 cut(s) 37, 79, 124, 166, 367
ApeKI GCWGC 4 cut(s) 102, 105, 108, 111
ApoI RAATTY 1 cut(s) 631
AspS9I GGNCC 5 cut(s) 38, 92, 166, 367, 468
AsuHPI GGTGA 5 cut(s) 139, 197, 232, 289, 385
AvaII GGWCC 2 cut(s) 92, 468
BbsI GAAGAC 3 cut(s) 124, 151, 175
BbvI GCAGC 4 cut(s) 89, 92, 95, 123
BccI CCATC 4 cut(s) 49, 161, 182, 308
BceAI ACGGC 2 cut(s) 52, 100
BcoDI GTCTC 1 cut(s) 644
BfmI CTRYAG 1 cut(s) 109
BisI GCNGC 4 cut(s) 103, 106, 109, 112
BlsI GCNGC 4 cut(s) 104, 107, 110, 113
Bme18I GGWCC 2 cut(s) 92, 468
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 5 cut(s) 38, 92, 166, 367, 468
BmiI GGNNCC 4 cut(s) 28, 94, 469, 612
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 2 cut(s) 336, 351
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 3 cut(s) 124, 151, 175
BplI GAGNNNNNCTC 2 cut(s) 81, 113
BsaHI GRCGYC 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 644
BsaJI CCNNGG 3 cut(s) 82, 97, 406
BsaWI WCCGGW 1 cut(s) 572
Bse1I ACTGG 1 cut(s) 244
BseDI CCNNGG 3 cut(s) 82, 97, 406
BseGI GGATG 2 cut(s) 41, 300
BseMII CTCAG 1 cut(s) 273
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 1 cut(s) 247
BseXI GCAGC 4 cut(s) 89, 92, 95, 123
BseYI CCCAGC 1 cut(s) 506
Bsh1285I CGRYCG 2 cut(s) 340, 562
BshFI GGCC 5 cut(s) 39, 81, 126, 168, 369
BsiEI CGRYCG 2 cut(s) 340, 562
BsiSI CCGG 1 cut(s) 573
BslFI GGGAC 1 cut(s) 78
BsmAI GTCTC 1 cut(s) 644
BsmFI GGGAC 1 cut(s) 78
BsnI GGCC 5 cut(s) 39, 81, 126, 168, 369
Bso31I GGTCTC 1 cut(s) 644
Bsp143I GATC 2 cut(s) 189, 384
BspACI CCGC 3 cut(s) 87, 150, 340
BspANI GGCC 5 cut(s) 39, 81, 126, 168, 369
BspCNI CTCAG 1 cut(s) 272
BspLI GGNNCC 4 cut(s) 28, 94, 469, 612
BspMAI CTGCAG 1 cut(s) 113
BspPI GGATC 1 cut(s) 197
BspTNI GGTCTC 1 cut(s) 644
BsrI ACTGG 1 cut(s) 244
BssECI CCNNGG 3 cut(s) 82, 97, 406
BssMI GATC 2 cut(s) 189, 384
BssNI GRCGYC 1 cut(s) 231
BssT1I CCWWGG 1 cut(s) 406
Bst4CI ACNGT 1 cut(s) 209
BstACI GRCGYC 1 cut(s) 231
BstDEI CTNAG 1 cut(s) 259
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 2 cut(s) 41, 300
BstKTI GATC 2 cut(s) 192, 387
BstMAI GTCTC 1 cut(s) 644
BstMBI GATC 2 cut(s) 189, 384
BstMCI CGRYCG 2 cut(s) 340, 562
BstMWI GCNNNNNNNGC 2 cut(s) 108, 111
BstSFI CTRYAG 1 cut(s) 109
BstV1I GCAGC 4 cut(s) 89, 92, 95, 123
BstV2I GAAGAC 3 cut(s) 124, 151, 175
BsuRI GGCC 5 cut(s) 39, 81, 126, 168, 369
BtgI CCRYGG 1 cut(s) 82
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 2 cut(s) 41, 300
BtsIMutI CAGTG 1 cut(s) 225
Cfr13I GGNCC 5 cut(s) 38, 92, 166, 367, 468
CseI GACGC 2 cut(s) 64, 239
Csp6I GTAC 1 cut(s) 621
CviAII CATG 2 cut(s) 163, 538
CviQI GTAC 1 cut(s) 621
DdeI CTNAG 1 cut(s) 259
DpnI GATC 2 cut(s) 191, 386
DpnII GATC 2 cut(s) 189, 384
EaeI YGGCCR 1 cut(s) 79
EciI GGCGGA 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 406
Eco31I GGTCTC 1 cut(s) 644
Eco47I GGWCC 2 cut(s) 92, 468
EcoO109I RGGNCCY 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 2 cut(s) 166, 541
FaiI YATR 4 cut(s) 164, 194, 363, 539
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FaqI GGGAC 1 cut(s) 78
FatI CATG 2 cut(s) 162, 537
Fnu4HI GCNGC 4 cut(s) 103, 106, 109, 112
FokI GGATG 2 cut(s) 28, 287
Fsp4HI GCNGC 4 cut(s) 103, 106, 109, 112
GluI GCNGC 4 cut(s) 103, 106, 109, 112
GsaI CCCAGC 1 cut(s) 510
HaeIII GGCC 5 cut(s) 39, 81, 126, 168, 369
HapII CCGG 1 cut(s) 573
HgaI GACGC 2 cut(s) 64, 239
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 2 cut(s) 166, 541
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 3 cut(s) 62, 544, 564
HpaII CCGG 1 cut(s) 573
HphI GGTGA 5 cut(s) 139, 197, 232, 289, 385
Hpy166II GTNNAC 1 cut(s) 514
Hpy188I TCNGA 4 cut(s) 7, 262, 305, 484
Hpy188III TCNNGA 2 cut(s) 187, 568
Hpy8I GTNNAC 1 cut(s) 514
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 1 cut(s) 601
HpyCH4III ACNGT 1 cut(s) 209
HpyCH4IV ACGT 2 cut(s) 222, 426
HpyCH4V TGCA 3 cut(s) 111, 478, 638
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 111
HpyF3I CTNAG 1 cut(s) 259
HpySE526I ACGT 2 cut(s) 222, 426
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 2 cut(s) 166, 541
Kzo9I GATC 2 cut(s) 189, 384
LmnI GCTCC 1 cut(s) 26
LpnPI CCDG 7 cut(s) 172, 257, 364, 383, 492, 586, 601
Lsp1109I GCAGC 4 cut(s) 89, 92, 95, 123
LweI GCATC 2 cut(s) 336, 351
MaeII ACGT 2 cut(s) 222, 426
MaeIII GTNAC 2 cut(s) 223, 599
MalI GATC 2 cut(s) 191, 386
MboI GATC 2 cut(s) 189, 384
MboII GAAGA 5 cut(s) 112, 124, 136, 151, 175
MluCI AATT 4 cut(s) 472, 519, 631, 679
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 462
MseI TTAA 1 cut(s) 444
MspA1I CMGCKG 1 cut(s) 114
MspI CCGG 1 cut(s) 573
MwoI GCNNNNNNNGC 2 cut(s) 108, 111
NdeII GATC 2 cut(s) 189, 384
NlaIII CATG 2 cut(s) 166, 541
NlaIV GGNNCC 4 cut(s) 28, 94, 469, 612
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 2 cut(s) 223, 599
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PfeI GAWTC 2 cut(s) 544, 564
PkrI GCNGC 4 cut(s) 104, 107, 110, 113
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PpuMI RGGWCCY 1 cut(s) 92
Psp1406I AACGTT 1 cut(s) 426
Psp5II RGGWCCY 1 cut(s) 92
PspFI CCCAGC 1 cut(s) 506
PspN4I GGNNCC 4 cut(s) 28, 94, 469, 612
PspPI GGNCC 5 cut(s) 38, 92, 166, 367, 468
PspPPI RGGWCCY 1 cut(s) 92
PstI CTGCAG 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 114
RsaI GTAC 1 cut(s) 622
RsaNI GTAC 1 cut(s) 621
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 4 cut(s) 103, 106, 109, 112
Sau3AI GATC 2 cut(s) 189, 384
Sau96I GGNCC 5 cut(s) 38, 92, 166, 367, 468
SchI GAGTC 1 cut(s) 71
SfaNI GCATC 2 cut(s) 336, 351
SfcI CTRYAG 1 cut(s) 109
SinI GGWCC 2 cut(s) 92, 468
Sse9I AATT 4 cut(s) 472, 519, 631, 679
SsiI CCGC 3 cut(s) 87, 150, 340
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 1 cut(s) 209
TaiI ACGT 2 cut(s) 225, 429
TaqI TCGA 2 cut(s) 345, 562
TaqII GACCGA 1 cut(s) 548
TasI AATT 4 cut(s) 472, 519, 631, 679
TfiI GAWTC 2 cut(s) 544, 564
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 1 cut(s) 232
TseFI GTSAC 2 cut(s) 223, 599
TseI GCWGC 4 cut(s) 102, 105, 108, 111
Tsp45I GTSAC 2 cut(s) 223, 599
TspDTI ATGAA 2 cut(s) 151, 542
TspRI CASTG 1 cut(s) 232
VpaK11BI GGWCC 2 cut(s) 92, 468
XapI RAATTY 1 cut(s) 631
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.