Rorug01G0063400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0010632
Physical Location & Seq
Forward (+)
10413863 .. 10418163
4301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0063400.1

Sequence Viewer

Length: 4071 bp
ATGGCAGTGGGAGAGATCTTTCTTGCGGCATTCCTTCAGTTGCTGCTGGAGAGGTTAACGCCTCAGGAGATCCTGGGCTACTTTGGAAGCTTGGGAGGAGCTGGCAAAAAGCTGCAGAAATGGAGAGAGACACTTTCCGCAGTTGCAGCCGTGCTTTACGATGCGGAGGAGAAGCAGCTGACGAACAAGGCAGTGGATTTGTGGCTGAATGACCTCAAACACTTGGCCTCTGATATGGACGATCTACTCGACACCTTTTCCACTGAGATGTTAGCACGCAAGCAGCTCAAGCTTCACCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCAAAGTTCCTCACAAAGTTAAATTCAACTTCAATATGAACTCTGAAATAGACGACATTGCTAAACGCTTGCAAGACATATTTGACCGGAAAGAGAAACTCGGTTTGAAATATATTGAGCATACCCCTACTTCAACATCATCATCGCACAGGACACCAAGTTCATATGTGTTGGATGGATCTGTGGTTGGGAGAGATGAAGACGCAGGAAAGATTGTGGAATTGTTGTCCAGAAATGCTGATCCTTCTTCTCCCACAAATTATCAAGTTGTTGCCATTGTTGGTATGGGTGGACTCGGCAAAACAACGCTTGCGGGACGAGTCTTCAATGATGTAGTTGCAATGAAACAGTTTGATCCCAAGGTCTGGGTTTCAGTATCCGATAACTTTGATCTTCAAACGGTGACGAGAGCTATTTTTAAGGAAGTTACATCTCGGTCTTGTGATATGGATGAGTTCAGTCGACTTCAAGATAATCTGAGCAAGGCGATCGATGGTAAAAGGTTTTTGATTGTTTTAGATGATGTCTGGAGCACATGTGATTATGATTCATGGACAAAACTGCAAGCTCCCCTCCGTGGCGGAGCCAAGGGAAGTAAGGTAATGGTCACAACACGTGACGAAAAAGTTGCAATATTGATGGGAGCCCCAGCCGGTGATGTTTATCATTTAAAGACTCTATCAGATGAAAGTTGTTTGCAAGTATTTGAGCAGCATGTTAGCAATGACAGGCCACCGAATTTTGAGTTGCTTAAGGAGAAAATTGTTACGAATTGCAATGGATTGCCATTGGCTGCAAAAACTCTTGGTGGTGTTTTACGTTGTGAAGAAACTGGCAAGTGGGAGGAAATACTGAATGACAAATTATGGAGTATGTCAGATAGGAGTAAGATACTCCCAGTACTGAGATTGAGTTATCATTATCTCCCTTCACCTTTGAAGAGGTGCTTTGCCTATTGCTCAATACTTCCCAATGACTATGAATTTGGGAAAACCCAATTAATCCTTCTGTGGATGGCAGAGGGTTTTCTTGAGCAACCAAAAGGGACTAAAGCAATGGAAGATATCGGTGACGAATATTTTGGGGAGCTATTGTCTCGGTCATTATTTCAAAACTCGGGCAAAAACAGTTTGTGCTTTGTAATGCATGACCTTGTTGGTGATTTGGCACGATGGGCTGCTGGAGACACATTTTGCAGATTGGAGGATAAACCGCATGGTAGATGTTCTCCAAACACTCGTCATCTGAGTTACATTTCTGCTAAGTTTGATGGGGAAAAAAGATTTGAGACATTTTCTGAAGCAAAAGGCTTGCGGACTTTCCTACCACTTCCCGTTTCCGGTGGTTATGAAAATTATCTGACTCGTTATGCTACTTCTGATTTATTGCCACAATTGAAATACTTGCGGGTGCTCTCCTTCAATGGCTATAAACTGACCGAGGTGCCAAATTCCGTAGGTAAGTTGAGACATCTACGGTACCTTGATCTTTCTCACACATTAATAACGTGTTTGCCTGAGTCAACATGCATGCTTTACAACTTACAGACGTTAATATTAGAGAATTGTTCAAAATTGAAGACACTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAACAATTCAAACATGCCTTCATTGGAAGAAATGCCTCCCAAAGTAAGTCGGTTGACTCATCTGCGAACTTTGCCTAATTTTGTGGTGGGGAAAGGTAGTGCATCAGGGATTGGAGAGATACAGTCATTGCATCTTCGTGGGACCTTGAGTGTTGGAAGATTGGAAAATGTGATTGATGTTGGGGATGCGAAGACGGCTCAAATAATGAACAAGGAAGGGCTGGAAATCTTGCAATTGGAATGGAGCGGCACAAGCGAGAAGGAGTTGGAGGTGCTGTGCAGTTTAGAACCTCATAAAAAGCTTAAGGAGCTGACCATCAAGGGCTACAATGGTTTCGAATTTTCAAAATGGATTGGACATCCTTCATTCTCTGATATGACGTCTGTGAAATTGGAGAATTGTAAAAACTGTCGATTCTTACCTCCTCTTGGCCAATTACCTTTTCTGAAAAATCTTCAGATACAAGGACTGGTCAATGTTGAAAGTGTGGGTGCTGAGTTTTATGGAGAGTGCAGCTTGCCTTTTCCAGTGTTGGAATGCATGACGTTCATGGATATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAAGAGATGAAGAAATTCGAGTTTTCCCATGCATGGAAAAGCTTTCCATCCTTGGTTGCCCCAAATTGAAAGATTTGTTGCCCAAGAGCATGGATTCATTATCAGAGCTGTATATTGCTGCATGTGAAGAGTTAGTGGTTTCAATTGCCAATTATAAACATCTTCGTGGGCTGTACATCTGCGGTTGTAAAAGGGTGGTGCACAGAAGTGGAGTTAAGTTTGAGTCACTGGAGGATATGAGGCTTTATAATATTTCAGAGTTCAGACTCGAAATAGACGGGTTCATCAGAGGATTGCCAAAGCTTCAAAAATTGTGGATTACTGATTGTGAAGAGTTGACATGTTTATGGGAGAATGAGGATAGATGGCTGCAACGTGGGATTTCTAATATTATTGGAGGCAACATGTCTGACCCCCCCTCTCACTTTATTCAAGAGCTAAGAGCACTCAAGGAACTTGTGCTAACTGGATGCTCAAATCTAATTTCTTTTCCAGAAGCTAGTTGGCCACCTTGTCTTGAAAATGTAAAGATGGAGTCGTGTAATTCTTTGACATATTTCGTAAGGTATCAAGTACCGCCAAGCATAAGAAGAATAGAGATAGAAAAATGTCAAAATTTGAAACTACTAGTGAAGGATATTGCTGGGGAGCTAGAGGGTTGTAATATGGAGTGGTTGGAGATAAAAGAGTGTGCATCTTTGACATCCTTATCAGGCAAAGGAGGCCGACTACCCAGAACCCTCAAATTTCTTAGAATAAGTGACTGTGAACGATTGGAGTCAATAATCAAAACATTCCACGAGGACACTTGTCTTGAAGCTCTTACGATAGAGAGGTGTGCAAATCTCAAATCCTTACCAGAGGGGCTATGCCATCTCTCCGCTCTTCGCATGTTATGGATAGAGGAATGTGGAAGTCTCGTTTCCTTCCCAACAGGAGGCTTGCCATCCAACCTGGCATGCTTGTCTATCTCCATTTGTGTTCAATTGGAAGCCCTCCCCAGAGGCATGGACAACCTCAGCTCTCTTCAGATTTTGATATTGCGATGCTGTGGAGGTTTGGCATCCATTCTAGAAGAGGGTTTCCCGCCCAACCTAATTAATCTGGAAATTGGCCCTCTCTCAGAGTGGGGGCTTCACCACTTGGACAGACTCACCTCTCTTGAAACCTTTTCTATCTACTGTGTAGATCCAGATCTGGTGTCCTTTCCGCTGCTCCCTAAATCTCTCGTTAAACTCACCATTGCAGGCTTCCCAAATCTGCAGCGCCTGTCATCATCTCTTCAATCACTCACCTCTCTTGAATCCCTTGACATTCTTGGATGCCCAAAGCTAGCATCTATTATTCCAGAAAAAGAGGATCATCTGCCTCCTTCACTTACACAGCTTCGCATCCAGGACGGCTGTCCGCTGCTCACAGAGAAATACCAACCAGGTAAAGCACGACACTGGCCCAAACAAATTGCCCACATCCCTTACGTTTATGTTGGAGGCTGCGATGACGAAGCCGAAGCCGACCGCCGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1356

Amino Acids

152.44

Weight (kDa)

6.09

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 99 1.9e-18 Rx N-terminal domain
NB-ARC PF00931 179 - 352 6.1e-38 NB-ARC domain
WHD_DRP PF23559 432 - 500 4.3e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 573 - 838 9.2e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 689 - 808 9e-34 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2697, 2790
AatII GACGTC 1 cut(s) 2339
Acc65I GGTACC 1 cut(s) 1809
AccB1I GGYRCC 2 cut(s) 1774, 1809
AccB7I CCANNNNNTGG 1 cut(s) 696
AccBSI CCGCTC 2 cut(s) 2202, 3425
AccI GTMKAC 1 cut(s) 793
AclWI GGATC 6 cut(s) 64, 517, 566, 680, 3725, 3909
AcoI YGGCCR 2 cut(s) 2386, 3047
AcsI RAATTY 8 cut(s) 353, 1069, 1313, 1780, 2294, 2556, 3157, 3287
AcuI CTGAAG 4 cut(s) 20, 1650, 2396, 3554
AcvI CACGTG 1 cut(s) 947
AcyI GRCGYC 1 cut(s) 2336
AfaI GTAC 5 cut(s) 318, 1233, 1811, 2717, 3117
AfiI CCNNNNNNNGG 7 cut(s) 696, 908, 1670, 2384, 2575, 3479, 4062
AflII CTTAAG 2 cut(s) 1082, 2258
AflIII ACRYGT 5 cut(s) 866, 944, 1838, 2882, 2946
AhlI ACTAGT 1 cut(s) 3169
AjnI CCWGG 4 cut(s) 72, 3495, 3936, 3973
AjuI GAANNNNNNNTTGG 4 cut(s) 1320, 1352, 2382, 2414
AleI CACNNNNGTG 1 cut(s) 2748
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
Alw21I GWGCWC 4 cut(s) 866, 1746, 2745, 2989
Alw26I GTCTC 6 cut(s) 122, 1431, 1509, 1613, 1792, 3464
Alw44I GTGCAC 1 cut(s) 2741
AlwI GGATC 6 cut(s) 64, 517, 566, 680, 3725, 3909
AlwNI CAGNNNCTG 2 cut(s) 43, 3811
Ama87I CYCGRG 1 cut(s) 1447
AoxI GGCC 7 cut(s) 225, 1061, 2386, 3047, 3265, 3655, 3992
ApaLI GTGCAC 1 cut(s) 2741
ApoI RAATTY 8 cut(s) 353, 1069, 1313, 1780, 2294, 2556, 3157, 3287
ArsI GACNNNNNNTTYG 4 cut(s) 1298, 1330, 1394, 1426
AseI ATTAAT 3 cut(s) 1331, 1832, 3642
Asp700I GAANNNNTTC 1 cut(s) 2556
Asp718I GGTACC 1 cut(s) 1809
AspLEI GCGC 1 cut(s) 3810
AspS9I GGNCC 3 cut(s) 2097, 3656, 3993
AsuC2I CCSGG 1 cut(s) 4064
AsuII TTCGAA 1 cut(s) 2292
AsuNHI GCTAGC 1 cut(s) 3874
AvaI CYCGRG 1 cut(s) 1447
AvaII GGWCC 1 cut(s) 2097
AxyI CCTNAGG 1 cut(s) 63
BaeGI GKGCMC 1 cut(s) 2745
BalI TGGCCA 2 cut(s) 2388, 3049
BanI GGYRCC 2 cut(s) 1774, 1809
BanII GRGCYC 1 cut(s) 979
BauI CACGAG 1 cut(s) 3341
BbrPI CACGTG 1 cut(s) 947
BbsI GAAGAC 4 cut(s) 537, 646, 1916, 2153
Bbv12I GWGCWC 4 cut(s) 866, 1746, 2745, 2989
BbvCI CCTCAGC 1 cut(s) 3560
BceAI ACGGC 3 cut(s) 134, 2166, 3958
BcgI CGANNNNNNTGC 4 cut(s) 802, 836, 941, 975
BciT130I CCWGG 4 cut(s) 74, 3497, 3938, 3975
BciVI GTATCC 1 cut(s) 718
BcnI CCSGG 1 cut(s) 4064
BcoDI GTCTC 6 cut(s) 122, 1431, 1509, 1613, 1792, 3464
BcuI ACTAGT 1 cut(s) 3169
BfaI CTAG 5 cut(s) 3042, 3170, 3194, 3614, 3875
BfmI CTRYAG 2 cut(s) 113, 3803
BfoI RGCGCY 1 cut(s) 3811
BfrI CTTAAG 2 cut(s) 1082, 2258
BfuI GTATCC 1 cut(s) 718
BglII AGATCT 2 cut(s) 15, 3736
BmcAI AGTACT 1 cut(s) 1233
Bme1390I CCNGG 5 cut(s) 74, 3497, 3938, 3975, 4064
Bme18I GGWCC 1 cut(s) 2097
BmeT110I CYCGRG 1 cut(s) 1447
BmgT120I GGNCC 3 cut(s) 2097, 3656, 3993
BmiI GGNNCC 5 cut(s) 916, 976, 1776, 1811, 2098
BmrFI CCNGG 5 cut(s) 74, 3497, 3938, 3975, 4064
BmrI ACTGGG 1 cut(s) 1223
BmtI GCTAGC 1 cut(s) 3878
BmuI ACTGGG 1 cut(s) 1223
BoxI GACNNNNGTC 2 cut(s) 3351, 3945
BpiI GAAGAC 4 cut(s) 537, 646, 1916, 2153
BpmI CTGGAG 4 cut(s) 68, 880, 1533, 2792
Bpu10I CCTNAGC 1 cut(s) 3560
Bpu14I TTCGAA 1 cut(s) 2292
BpuEI CTTGAG 4 cut(s) 272, 1382, 2122, 2975
BpuMI CCSGG 1 cut(s) 4064
Bsa29I ATCGAT 1 cut(s) 822
BsaAI YACGTR 1 cut(s) 947
BsaBI GATNNNNATC 2 cut(s) 993, 3735
BsaHI GRCGYC 1 cut(s) 2336
BsaJI CCNNGG 6 cut(s) 73, 690, 907, 918, 1770, 2593
BsaWI WCCGGW 2 cut(s) 417, 1670
BsaXI ACNNNNNCTCC 6 cut(s) 853, 883, 1409, 1439, 2216, 2246
Bsc4I CCNNNNNNNGG 7 cut(s) 696, 908, 1670, 2384, 2575, 3479, 4062
Bse118I RCCGGY 1 cut(s) 983
Bse1I ACTGG 8 cut(s) 307, 1168, 1229, 2430, 2483, 2775, 3013, 3995
Bse21I CCTNAGG 1 cut(s) 63
Bse3DI GCAATG 7 cut(s) 387, 678, 1060, 1114, 1392, 2081, 3783
Bse8I GATNNNNATC 2 cut(s) 993, 3735
BseBI CCWGG 4 cut(s) 74, 3497, 3938, 3975
BseCI ATCGAT 1 cut(s) 822
BseDI CCNNGG 6 cut(s) 73, 690, 907, 918, 1770, 2593
BseJI GATNNNNATC 2 cut(s) 993, 3735
BseLI CCNNNNNNNGG 7 cut(s) 696, 908, 1670, 2384, 2575, 3479, 4062
BseMI GCAATG 7 cut(s) 387, 678, 1060, 1114, 1392, 2081, 3783
BseMII CTCAG 9 cut(s) 77, 255, 800, 1226, 1568, 1839, 2442, 3574, 3678
BseNI ACTGG 8 cut(s) 307, 1168, 1229, 2430, 2483, 2775, 3013, 3995
BseRI GAGGAG 3 cut(s) 111, 182, 2370
BseSI GKGCMC 1 cut(s) 2745
BseYI CCCAGC 2 cut(s) 979, 3185
BsgI GTGCAG 2 cut(s) 2254, 2488
Bsh1285I CGRYCG 2 cut(s) 822, 4060
BshFI GGCC 7 cut(s) 227, 1063, 2388, 3049, 3267, 3657, 3994
BshNI GGYRCC 2 cut(s) 1774, 1809
BshVI ATCGAT 1 cut(s) 822
BsiEI CGRYCG 2 cut(s) 822, 4060
BsiHKAI GWGCWC 4 cut(s) 866, 1746, 2745, 2989
BsiHKCI CYCGRG 1 cut(s) 1447
BsiSI CCGG 4 cut(s) 418, 984, 1671, 4063
BslFI GGGAC 3 cut(s) 660, 1390, 2110
BslI CCNNNNNNNGG 7 cut(s) 696, 908, 1670, 2384, 2575, 3479, 4062
BsmAI GTCTC 6 cut(s) 122, 1431, 1509, 1613, 1792, 3464
BsmFI GGGAC 3 cut(s) 660, 1390, 2110
BsmI GAATGC 2 cut(s) 29, 2498
BsnI GGCC 7 cut(s) 227, 1063, 2388, 3049, 3267, 3657, 3994
BsoBI CYCGRG 1 cut(s) 1447
Bsp119I TTCGAA 1 cut(s) 2292
Bsp1286I GDGCHC 5 cut(s) 866, 979, 1746, 2745, 2989
Bsp1407I TGTACA 1 cut(s) 2715
BspANI GGCC 7 cut(s) 227, 1063, 2388, 3049, 3267, 3657, 3994
BspCNI CTCAG 9 cut(s) 76, 256, 801, 1227, 1569, 1840, 2443, 3573, 3677
BspDI ATCGAT 1 cut(s) 822
BspLI GGNNCC 5 cut(s) 916, 976, 1776, 1811, 2098
BspMAI CTGCAG 2 cut(s) 117, 3807
BspOI GCTAGC 1 cut(s) 3878
BspPI GGATC 6 cut(s) 64, 517, 566, 680, 3725, 3909
BspQI GCTCTTC 1 cut(s) 3432
BspT104I TTCGAA 1 cut(s) 2292
BspT107I GGYRCC 2 cut(s) 1774, 1809
BspTI CTTAAG 2 cut(s) 1082, 2258
BsrBI CCGCTC 2 cut(s) 2202, 3425
BsrDI GCAATG 7 cut(s) 387, 678, 1060, 1114, 1392, 2081, 3783
BsrFI RCCGGY 1 cut(s) 983
BsrGI TGTACA 1 cut(s) 2715
BsrI ACTGG 8 cut(s) 307, 1168, 1229, 2430, 2483, 2775, 3013, 3995
BssAI RCCGGY 1 cut(s) 983
BssECI CCNNGG 6 cut(s) 73, 690, 907, 918, 1770, 2593
BssNI GRCGYC 1 cut(s) 2336
BssSI CACGAG 1 cut(s) 3341
BssT1I CCWWGG 3 cut(s) 690, 918, 2593
Bst2BI CACGAG 1 cut(s) 3341
Bst2UI CCWGG 4 cut(s) 74, 3497, 3938, 3975
Bst4CI ACNGT 8 cut(s) 681, 733, 1460, 1809, 2079, 2366, 3308, 3725
Bst6I CTCTTC 7 cut(s) 1265, 2664, 2868, 3432, 3573, 3612, 3828
BstACI GRCGYC 1 cut(s) 2336
BstAFI CTTAAG 2 cut(s) 1082, 2258
BstAUI TGTACA 1 cut(s) 2715
BstBAI YACGTR 1 cut(s) 947
BstBI TTCGAA 1 cut(s) 2292
BstDSI CCRYGG 1 cut(s) 907
BstH2I RGCGCY 1 cut(s) 3811
BstHHI GCGC 1 cut(s) 3810
BstMAI GTCTC 6 cut(s) 122, 1431, 1509, 1613, 1792, 3464
BstMCI CGRYCG 2 cut(s) 822, 4060
BstMWI GCNNNNNNNGC 8 cut(s) 146, 289, 1505, 2026, 2150, 2208, 2263, 3264
BstNI CCWGG 4 cut(s) 74, 3497, 3938, 3975
BstPAI GACNNNNGTC 2 cut(s) 3351, 3945
BstSCI CCNGG 5 cut(s) 72, 3495, 3936, 3973, 4062
BstSFI CTRYAG 2 cut(s) 113, 3803
BstSLI GKGCMC 1 cut(s) 2745
BstV2I GAAGAC 4 cut(s) 537, 646, 1916, 2153
BstX2I RGATCY 5 cut(s) 15, 69, 509, 3730, 3736
BstXI CCANNNNNNTGG 2 cut(s) 2632, 3550
BstYI RGATCY 5 cut(s) 15, 69, 509, 3730, 3736
Bsu15I ATCGAT 1 cut(s) 822
Bsu36I CCTNAGG 1 cut(s) 63
BsuI GTATCC 1 cut(s) 718
BsuRI GGCC 7 cut(s) 227, 1063, 2388, 3049, 3267, 3657, 3994
BsuTUI ATCGAT 1 cut(s) 822
BtgI CCRYGG 1 cut(s) 907
BtgZI GCGATG 3 cut(s) 459, 3601, 4053
BtsI GCAGTG 2 cut(s) 12, 198
BtsIMutI CAGTG 6 cut(s) 12, 198, 261, 2490, 2768, 3988
CaiI CAGNNNCTG 2 cut(s) 43, 3811
CfoI GCGC 1 cut(s) 3810
Cfr10I RCCGGY 1 cut(s) 983
Cfr13I GGNCC 3 cut(s) 2097, 3656, 3993
ClaI ATCGAT 1 cut(s) 822
CseI GACGC 1 cut(s) 542
CsiI ACCWGGT 1 cut(s) 3973
Csp6I GTAC 5 cut(s) 317, 1232, 1810, 2716, 3116
CspCI CAANNNNNGTGG 2 cut(s) 2837, 2872
CviQI GTAC 5 cut(s) 317, 1232, 1810, 2716, 3116
DraI TTTAAA 1 cut(s) 1002
EaeI YGGCCR 2 cut(s) 2386, 3047
Eam1104I CTCTTC 7 cut(s) 1265, 2664, 2868, 3432, 3573, 3612, 3828
EarI CTCTTC 7 cut(s) 1265, 2664, 2868, 3432, 3573, 3612, 3828
EciI GGCGGA 1 cut(s) 927
Eco130I CCWWGG 3 cut(s) 690, 918, 2593
Eco24I GRGCYC 1 cut(s) 979
Eco32I GATATC 1 cut(s) 1396
Eco47I GGWCC 1 cut(s) 2097
Eco57I CTGAAG 4 cut(s) 20, 1650, 2396, 3554
Eco72I CACGTG 1 cut(s) 947
Eco81I CCTNAGG 1 cut(s) 63
Eco88I CYCGRG 1 cut(s) 1447
EcoO109I RGGNCCY 1 cut(s) 2097
EcoRII CCWGG 4 cut(s) 72, 3495, 3936, 3973
EcoRV GATATC 1 cut(s) 1396
EcoT14I CCWWGG 3 cut(s) 690, 918, 2593
EcoT22I ATGCAT 4 cut(s) 1479, 1862, 2498, 2576
EcoT38I GRGCYC 1 cut(s) 979
ErhI CCWWGG 3 cut(s) 690, 918, 2593
FalI AAGNNNNNCTT 2 cut(s) 1262, 1294
FaqI GGGAC 3 cut(s) 660, 1390, 2110
FauI CCCGC 3 cut(s) 637, 1731, 3636
FauNDI CATATG 1 cut(s) 496
FblI GTMKAC 1 cut(s) 793
FriOI GRGCYC 1 cut(s) 979
FspBI CTAG 5 cut(s) 3042, 3170, 3194, 3614, 3875
GlaI GCGC 1 cut(s) 3809
GsaI CCCAGC 2 cut(s) 983, 3189
GsuI CTGGAG 4 cut(s) 68, 880, 1533, 2792
HaeII RGCGCY 1 cut(s) 3811
HaeIII GGCC 7 cut(s) 227, 1063, 2388, 3049, 3267, 3657, 3994
HapII CCGG 4 cut(s) 418, 984, 1671, 4063
HgaI GACGC 1 cut(s) 542
HhaI GCGC 1 cut(s) 3810
Hin1I GRCGYC 1 cut(s) 2336
Hin6I GCGC 1 cut(s) 3808
HinP1I GCGC 1 cut(s) 3808
HincII GTYRAC 5 cut(s) 57, 794, 1854, 2010, 2880
HindII GTYRAC 5 cut(s) 57, 794, 1854, 2010, 2880
HindIII AAGCTT 5 cut(s) 88, 290, 2255, 2582, 2843
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 4 cut(s) 418, 984, 1671, 4063
Hpy166II GTNNAC 8 cut(s) 57, 623, 794, 1854, 2010, 2743, 2880, 3311
Hpy8I GTNNAC 8 cut(s) 57, 623, 794, 1854, 2010, 2743, 2880, 3311
HpyCH4III ACNGT 8 cut(s) 681, 733, 1460, 1809, 2079, 2366, 3308, 3725
HpyCH4IV ACGT 9 cut(s) 946, 1150, 1838, 1880, 1948, 2336, 2501, 2917, 4020
HpyF10VI GCNNNNNNNGC 8 cut(s) 146, 289, 1505, 2026, 2150, 2208, 2263, 3264
HpySE526I ACGT 9 cut(s) 946, 1150, 1838, 1880, 1948, 2336, 2501, 2917, 4020
Hsp92I GRCGYC 1 cut(s) 2336
HspAI GCGC 1 cut(s) 3808
KpnI GGTACC 1 cut(s) 1813
KspAI GTTAAC 1 cut(s) 57
LguI GCTCTTC 1 cut(s) 3432
MabI ACCWGGT 1 cut(s) 3973
MaeI CTAG 5 cut(s) 3042, 3170, 3194, 3614, 3875
MaeII ACGT 9 cut(s) 946, 1150, 1838, 1880, 1948, 2336, 2501, 2917, 4020
MaeIII GTNAC 9 cut(s) 733, 757, 937, 947, 1096, 1400, 1580, 2766, 3302
MbiI CCGCTC 2 cut(s) 2202, 3425
MfeI CAATTG 4 cut(s) 1724, 2189, 2685, 3527
MflI RGATCY 5 cut(s) 15, 69, 509, 3730, 3736
MhlI GDGCHC 5 cut(s) 866, 979, 1746, 2745, 2989
MlsI TGGCCA 2 cut(s) 2388, 3049
MluNI TGGCCA 2 cut(s) 2388, 3049
MmeI TCCRAC 7 cut(s) 483, 2089, 2202, 2469, 3198, 3516, 4009
Mox20I TGGCCA 2 cut(s) 2388, 3049
Mph1103I ATGCAT 4 cut(s) 1479, 1862, 2498, 2576
MroXI GAANNNNTTC 1 cut(s) 2556
MscI TGGCCA 2 cut(s) 2388, 3049
MslI CAYNNNNRTG 6 cut(s) 266, 2091, 2510, 2706, 2748, 2887
Msp20I TGGCCA 2 cut(s) 2388, 3049
MspA1I CMGCKG 3 cut(s) 178, 3754, 3952
MspCI CTTAAG 2 cut(s) 1082, 2258
MspI CCGG 4 cut(s) 418, 984, 1671, 4063
MspR9I CCNGG 5 cut(s) 74, 3497, 3938, 3975, 4064
MunI CAATTG 4 cut(s) 1724, 2189, 2685, 3527
Mva1269I GAATGC 2 cut(s) 29, 2498
MvaI CCWGG 4 cut(s) 74, 3497, 3938, 3975
MwoI GCNNNNNNNGC 8 cut(s) 146, 289, 1505, 2026, 2150, 2208, 2263, 3264
NciI CCSGG 1 cut(s) 4064
NdeI CATATG 1 cut(s) 496
NheI GCTAGC 1 cut(s) 3874
NlaIV GGNNCC 5 cut(s) 916, 976, 1776, 1811, 2098
NmeAIII GCCGAG 1 cut(s) 606
NmuCI GTSAC 6 cut(s) 733, 937, 947, 1400, 2766, 3302
NsiI ATGCAT 4 cut(s) 1479, 1862, 2498, 2576
NspV TTCGAA 1 cut(s) 2292
OliI CACNNNNGTG 1 cut(s) 2748
PaeI GCATGC 2 cut(s) 1864, 3504
PciI ACATGT 3 cut(s) 866, 2882, 2946
PciSI GCTCTTC 1 cut(s) 3432
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PctI GAATGC 2 cut(s) 29, 2498
PdmI GAANNNNTTC 1 cut(s) 2556
PfeI GAWTC 4 cut(s) 878, 2370, 2636, 3845
PflMI CCANNNNNTGG 1 cut(s) 696
PfoI TCCNGGA 1 cut(s) 3936
Ple19I CGATCG 1 cut(s) 822
PmaCI CACGTG 1 cut(s) 947
PmlI CACGTG 1 cut(s) 947
Ppu21I YACGTR 1 cut(s) 947
PpuMI RGGWCCY 1 cut(s) 2097
PscI ACATGT 3 cut(s) 866, 2882, 2946
PshAI GACNNNNGTC 2 cut(s) 3351, 3945
PshBI ATTAAT 3 cut(s) 1331, 1832, 3642
PsiI TTATAA 2 cut(s) 2697, 2790
Psp5II RGGWCCY 1 cut(s) 2097
Psp6I CCWGG 4 cut(s) 72, 3495, 3936, 3973
PspCI CACGTG 1 cut(s) 947
PspFI CCCAGC 2 cut(s) 979, 3185
PspGI CCWGG 4 cut(s) 72, 3495, 3936, 3973
PspN4I GGNNCC 5 cut(s) 916, 976, 1776, 1811, 2098
PspPI GGNCC 3 cut(s) 2097, 3656, 3993
PspPPI RGGWCCY 1 cut(s) 2097
PstI CTGCAG 2 cut(s) 117, 3807
PstNI CAGNNNCTG 2 cut(s) 43, 3811
PsuI RGATCY 5 cut(s) 15, 69, 509, 3730, 3736
PvuI CGATCG 1 cut(s) 822
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 5 cut(s) 318, 1233, 1811, 2717, 3117
RsaNI GTAC 5 cut(s) 317, 1232, 1810, 2716, 3116
RseI CAYNNNNRTG 6 cut(s) 266, 2091, 2510, 2706, 2748, 2887
SalI GTCGAC 1 cut(s) 792
SapI GCTCTTC 1 cut(s) 3432
Sau96I GGNCC 3 cut(s) 2097, 3656, 3993
ScaI AGTACT 1 cut(s) 1233
ScrFI CCNGG 5 cut(s) 74, 3497, 3938, 3975, 4064
SduI GDGCHC 5 cut(s) 866, 979, 1746, 2745, 2989
SexAI ACCWGGT 1 cut(s) 3973
SfcI CTRYAG 2 cut(s) 113, 3803
SfuI TTCGAA 1 cut(s) 2292
SinI GGWCC 1 cut(s) 2097
SmiMI CAYNNNNRTG 6 cut(s) 266, 2091, 2510, 2706, 2748, 2887
SmlI CTYRAG 6 cut(s) 287, 1082, 1361, 2101, 2258, 2990
SmoI CTYRAG 6 cut(s) 287, 1082, 1361, 2101, 2258, 2990
SpeI ACTAGT 1 cut(s) 3169
SphI GCATGC 2 cut(s) 1864, 3504
SspI AATATT 5 cut(s) 966, 1409, 1887, 2794, 2932
SspMI CTAG 5 cut(s) 3042, 3170, 3194, 3614, 3875
StyD4I CCNGG 5 cut(s) 72, 3495, 3936, 3973, 4062
StyI CCWWGG 3 cut(s) 690, 918, 2593
TaaI ACNGT 8 cut(s) 681, 733, 1460, 1809, 2079, 2366, 3308, 3725
TaiI ACGT 9 cut(s) 949, 1153, 1841, 1883, 1951, 2339, 2504, 2920, 4023
TaqI TCGA 7 cut(s) 249, 793, 822, 2292, 2368, 2560, 2811
TaqII GACCGA 3 cut(s) 756, 1419, 1784
TatI WGTACW 2 cut(s) 1231, 2715
TauI GCSGC 2 cut(s) 29, 2205
TfiI GAWTC 4 cut(s) 878, 2370, 2636, 3845
TscAI CASTG 6 cut(s) 12, 198, 268, 2490, 2775, 3995
TseFI GTSAC 6 cut(s) 733, 937, 947, 1400, 2766, 3302
Tsp45I GTSAC 6 cut(s) 733, 937, 947, 1400, 2766, 3302
TspGWI ACGGA 2 cut(s) 896, 1774
TspRI CASTG 6 cut(s) 12, 198, 268, 2490, 2775, 3995
Van91I CCANNNNNTGG 1 cut(s) 696
Vha464I CTTAAG 2 cut(s) 1082, 2258
VneI GTGCAC 1 cut(s) 2741
VpaK11BI GGWCC 1 cut(s) 2097
VspI ATTAAT 3 cut(s) 1331, 1832, 3642
XapI RAATTY 8 cut(s) 353, 1069, 1313, 1780, 2294, 2556, 3157, 3287
XbaI TCTAGA 1 cut(s) 3613
XcmI CCANNNNNNNNNTGG 2 cut(s) 613, 3042
XmiI GTMKAC 1 cut(s) 793
XmnI GAANNNNTTC 1 cut(s) 2556
XspI CTAG 5 cut(s) 3042, 3170, 3194, 3614, 3875
ZraI GACGTC 1 cut(s) 2337
ZrmI AGTACT 1 cut(s) 1233
Zsp2I ATGCAT 4 cut(s) 1479, 1862, 2498, 2576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.