Rorug01G0363300

Protein EARLY RESPONSIVE TO DEHYDRATION 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0012654
Physical Location & Seq
Reverse (-)
47865447 .. 47869050
3604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0363300.1

Sequence Viewer

Length: 1026 bp
ATGGCTACTAATGGAGAACATGCTTCTACCAAACCGCCTCCAACCCCCTCTCCTCTAAGGAATGCCAAATTCTTTCAGGCAAATATGAGAATTTTGGTTACTGGAGGAGCTGGATTCATTGGCTCTCACCTAGTCGACAAGCTGATGGAAAACGAGAAGAATGAGGTAATTGTTGCAGATAACTACTTCACTGGCTCAAAGGACAACCTTAAGAAATGGATTGGTCATCCAAGATTCGAGCTTATTCGTCATGATGTCACCGAGCCATTGCTGGTTGAGGTTGATCAAATATTCCATCTTGCTTGCCCTGCTTCTCCGATCTTCTACAAACACAATCCAGTAAAGACAATAAAGACAAATGTGATCGGTACATTGAACATGCTGGGACTGGCCAAGCGAGTTGGAGCGAGGATTTTGCTCACTTCTACTTCGGAGGTGTATGGAGATCCTCTTATTCACCCTCAGGTTGAGAGCTATTGGGGAAATGTGAATCCAATTGGAGTTAGGAGTTGCTATGATGAGGGAAAGCGAGTGGCTGAAACATTGATGTTTGATTATCACAGGCAGCACGGCATAGAGATACGGATTGCTAGGATTTTCAACACTTATGGACCTCGCATGAATATTGATGATGGCCGTGTCGTCAGCAACTTTATAGCCCAAGCTATCCGGAATGACCCCTTGACTGTTCAAGCGCCTGGAACACAGACAAGGAGTTTCTGCTACGTCTCTGACATGGTTGATGGCCTTATTCGACTTATGGAAGGAGACAACACTGGTCCAATCAACATTGGGAATCCAGGTGAATTTACAATGCTCGAACTCGCAGAGAATGTCAAGGAGCTTATCAATCCTAAGGTGGAGATTAAAATGGTTGAAAACACACCTGATGATCCTCGGCAGAGGAAGCCTGACATCTCAAAAGCAAAGGAGTTGCTGGGATGGGAACCAAAGGTCAAGTTGCGTGATGGTCTGCCCCTCATGGAAGATGATTTCCGTACAAGACTTGGTGTCCCCAGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAM2

Protein Analysis

341

Amino Acids

38.32

Weight (kDa)

7.13

Isoelectric Point (pI)

28.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 29 - 292 2.7e-07 RmlD substrate binding domain
Epimerase PF01370 31 - 265 1.5e-45 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 31 - 138 9.2e-08 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 32 - 326 1.4e-49 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 32 - 184 1.1e-12 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 87 - 201 3.5e-06 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AccIII TCCGGA 1 cut(s) 669
AciI CCGC 1 cut(s) 35
AclWI GGATC 2 cut(s) 440, 887
AcoI YGGCCR 2 cut(s) 390, 634
AcsI RAATTY 3 cut(s) 68, 90, 806
AfaI GTAC 2 cut(s) 370, 1000
AflII CTTAAG 1 cut(s) 209
AgsI TTSAA 4 cut(s) 376, 601, 692, 878
AhdI GACNNNNNGTC 1 cut(s) 1010
AjnI CCWGG 2 cut(s) 697, 799
AluBI AGCT 6 cut(s) 110, 142, 241, 474, 665, 844
AluI AGCT 6 cut(s) 110, 142, 241, 474, 665, 844
Alw26I GTCTC 2 cut(s) 733, 762
AlwI GGATC 2 cut(s) 440, 887
Aor13HI TCCGGA 1 cut(s) 669
AoxI GGCC 3 cut(s) 390, 634, 745
ApeKI GCWGC 1 cut(s) 565
ApoI RAATTY 3 cut(s) 68, 90, 806
AspLEI GCGC 1 cut(s) 697
AspS9I GGNCC 2 cut(s) 611, 779
AsuHPI GGTGA 4 cut(s) 119, 250, 449, 815
AvaII GGWCC 2 cut(s) 611, 779
AxyI CCTNAGG 2 cut(s) 462, 855
BalI TGGCCA 1 cut(s) 392
BbvI GCAGC 1 cut(s) 577
BccI CCATC 6 cut(s) 139, 303, 626, 737, 936, 962
BceAI ACGGC 2 cut(s) 586, 621
BcgI CGANNNNNNTGC 2 cut(s) 397, 431
BciT130I CCWGG 2 cut(s) 699, 801
BclI TGATCA 1 cut(s) 283
BcoDI GTCTC 2 cut(s) 733, 762
BfaI CTAG 2 cut(s) 131, 591
BfoI RGCGCY 1 cut(s) 698
BfrI CTTAAG 1 cut(s) 209
BisI GCNGC 1 cut(s) 566
BlsI GCNGC 1 cut(s) 567
Bme1390I CCNGG 2 cut(s) 699, 801
Bme18I GGWCC 2 cut(s) 611, 779
BmeRI GACNNNNNGTC 1 cut(s) 1010
BmgT120I GGNCC 2 cut(s) 611, 779
BmiI GGNNCC 1 cut(s) 948
BmrFI CCNGG 2 cut(s) 699, 801
BpmI CTGGAG 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 896
BsaWI WCCGGW 1 cut(s) 669
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bse1I ACTGG 5 cut(s) 106, 196, 338, 393, 781
Bse21I CCTNAGG 2 cut(s) 462, 855
Bse3DI GCAATG 1 cut(s) 266
BseAI TCCGGA 1 cut(s) 669
BseBI CCWGG 2 cut(s) 699, 801
BseDI CCNNGG 1 cut(s) 896
BseGI GGATG 2 cut(s) 226, 947
BseMI GCAATG 1 cut(s) 266
BseMII CTCAG 1 cut(s) 476
BseNI ACTGG 5 cut(s) 106, 196, 338, 393, 781
BseRI GAGGAG 2 cut(s) 42, 120
BseXI GCAGC 1 cut(s) 577
BseYI CCCAGC 2 cut(s) 382, 937
BshFI GGCC 3 cut(s) 392, 636, 747
BsiSI CCGG 1 cut(s) 670
BslFI GGGAC 2 cut(s) 399, 998
BsmAI GTCTC 2 cut(s) 733, 762
BsmBI CGTCTC 1 cut(s) 733
BsmFI GGGAC 2 cut(s) 399, 998
BsmI GAATGC 1 cut(s) 67
BsnI GGCC 3 cut(s) 392, 636, 747
Bsp13I TCCGGA 1 cut(s) 669
Bsp143I GATC 5 cut(s) 283, 318, 363, 445, 892
BspACI CCGC 1 cut(s) 35
BspANI GGCC 3 cut(s) 392, 636, 747
BspCNI CTCAG 1 cut(s) 475
BspEI TCCGGA 1 cut(s) 669
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 1 cut(s) 948
BspPI GGATC 2 cut(s) 440, 887
BspTI CTTAAG 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 266
BsrI ACTGG 5 cut(s) 106, 196, 338, 393, 781
BssECI CCNNGG 1 cut(s) 896
BssMI GATC 5 cut(s) 283, 318, 363, 445, 892
Bst2UI CCWGG 2 cut(s) 699, 801
Bst4CI ACNGT 1 cut(s) 688
BstAFI CTTAAG 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 3 cut(s) 56, 462, 855
BstF5I GGATG 2 cut(s) 226, 947
BstH2I RGCGCY 1 cut(s) 698
BstHHI GCGC 1 cut(s) 697
BstKTI GATC 5 cut(s) 286, 321, 366, 448, 895
BstMAI GTCTC 2 cut(s) 733, 762
BstMBI GATC 5 cut(s) 283, 318, 363, 445, 892
BstMWI GCNNNNNNNGC 2 cut(s) 308, 907
BstNI CCWGG 2 cut(s) 699, 801
BstNSI RCATGY 2 cut(s) 23, 382
BstSCI CCNGG 2 cut(s) 697, 799
BstV1I GCAGC 1 cut(s) 577
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
Bsu36I CCTNAGG 2 cut(s) 462, 855
BsuRI GGCC 3 cut(s) 392, 636, 747
BtsCI GGATG 2 cut(s) 226, 947
BtsIMutI CAGTG 2 cut(s) 189, 774
Cac8I GCNNGC 1 cut(s) 304
CciI TCATGA 1 cut(s) 250
CfoI GCGC 1 cut(s) 697
Cfr13I GGNCC 2 cut(s) 611, 779
Csp6I GTAC 2 cut(s) 369, 999
CviAII CATG 6 cut(s) 20, 251, 379, 619, 736, 982
CviQI GTAC 2 cut(s) 369, 999
DdeI CTNAG 3 cut(s) 56, 462, 855
DpnI GATC 5 cut(s) 285, 320, 365, 447, 894
DpnII GATC 5 cut(s) 283, 318, 363, 445, 892
DriI GACNNNNNGTC 1 cut(s) 1010
EaeI YGGCCR 2 cut(s) 390, 634
Eam1105I GACNNNNNGTC 1 cut(s) 1010
Eco47I GGWCC 2 cut(s) 611, 779
Eco81I CCTNAGG 2 cut(s) 462, 855
EcoRII CCWGG 2 cut(s) 697, 799
Esp3I CGTCTC 1 cut(s) 733
FaeI CATG 6 cut(s) 23, 254, 382, 622, 739, 985
FaqI GGGAC 2 cut(s) 399, 998
FatI CATG 6 cut(s) 19, 250, 378, 618, 735, 981
FbaI TGATCA 1 cut(s) 283
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 1 cut(s) 566
FokI GGATG 2 cut(s) 213, 954
Fsp4HI GCNGC 1 cut(s) 566
FspBI CTAG 2 cut(s) 131, 591
GlaI GCGC 1 cut(s) 696
GluI GCNGC 1 cut(s) 566
GsaI CCCAGC 2 cut(s) 386, 941
GsuI CTGGAG 1 cut(s) 123
HaeII RGCGCY 1 cut(s) 698
HaeIII GGCC 3 cut(s) 392, 636, 747
HapII CCGG 1 cut(s) 670
HhaI GCGC 1 cut(s) 697
Hin1II CATG 6 cut(s) 23, 254, 382, 622, 739, 985
Hin6I GCGC 1 cut(s) 695
HinP1I GCGC 1 cut(s) 695
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 4 cut(s) 114, 234, 490, 796
HpaII CCGG 1 cut(s) 670
HphI GGTGA 4 cut(s) 119, 250, 449, 815
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 3 cut(s) 318, 433, 733
Hpy188III TCNNGA 2 cut(s) 251, 670
Hpy8I GTNNAC 1 cut(s) 136
HpyAV CCTTC 1 cut(s) 758
HpyCH4III ACNGT 1 cut(s) 688
HpyCH4IV ACGT 1 cut(s) 726
HpyCH4V TGCA 1 cut(s) 176
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 907
HpyF3I CTNAG 3 cut(s) 56, 462, 855
HpySE526I ACGT 1 cut(s) 726
Hsp92II CATG 6 cut(s) 23, 254, 382, 622, 739, 985
HspAI GCGC 1 cut(s) 695
Kpn2I TCCGGA 1 cut(s) 669
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 5 cut(s) 283, 318, 363, 445, 892
LmnI GCTCC 3 cut(s) 107, 404, 841
Lsp1109I GCAGC 1 cut(s) 577
MaeI CTAG 2 cut(s) 131, 591
MaeII ACGT 1 cut(s) 726
MaeIII GTNAC 2 cut(s) 97, 256
MalI GATC 5 cut(s) 285, 320, 365, 447, 894
MboI GATC 5 cut(s) 283, 318, 363, 445, 892
MboII GAAGA 3 cut(s) 169, 313, 998
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 1 cut(s) 445
MlsI TGGCCA 1 cut(s) 392
MluCI AATT 6 cut(s) 68, 90, 168, 495, 806, 1021
MluNI TGGCCA 1 cut(s) 392
MmeI TCCRAC 2 cut(s) 65, 382
Mox20I TGGCCA 1 cut(s) 392
MroI TCCGGA 1 cut(s) 669
MscI TGGCCA 1 cut(s) 392
MseI TTAA 3 cut(s) 210, 867, 1024
Msp20I TGGCCA 1 cut(s) 392
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 1 cut(s) 670
MspR9I CCNGG 2 cut(s) 699, 801
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 67
MvaI CCWGG 2 cut(s) 699, 801
MwoI GCNNNNNNNGC 2 cut(s) 308, 907
NdeII GATC 5 cut(s) 283, 318, 363, 445, 892
NlaIII CATG 6 cut(s) 23, 254, 382, 622, 739, 985
NlaIV GGNNCC 1 cut(s) 948
NmeAIII GCCGAG 1 cut(s) 877
NmuCI GTSAC 1 cut(s) 256
NspI RCATGY 2 cut(s) 23, 382
PagI TCATGA 1 cut(s) 250
PctI GAATGC 1 cut(s) 67
PfeI GAWTC 4 cut(s) 114, 234, 490, 796
PkrI GCNGC 1 cut(s) 567
Psp6I CCWGG 2 cut(s) 697, 799
PspFI CCCAGC 2 cut(s) 382, 937
PspGI CCWGG 2 cut(s) 697, 799
PspN4I GGNNCC 1 cut(s) 948
PspPI GGNCC 2 cut(s) 611, 779
PsuI RGATCY 1 cut(s) 445
RsaI GTAC 2 cut(s) 370, 1000
RsaNI GTAC 2 cut(s) 369, 999
SalI GTCGAC 1 cut(s) 134
SaqAI TTAA 3 cut(s) 210, 867, 1024
SatI GCNGC 1 cut(s) 566
Sau3AI GATC 5 cut(s) 283, 318, 363, 445, 892
Sau96I GGNCC 2 cut(s) 611, 779
ScrFI CCNGG 2 cut(s) 699, 801
SinI GGWCC 2 cut(s) 611, 779
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 6 cut(s) 68, 90, 168, 495, 806, 1021
SsiI CCGC 1 cut(s) 35
SspI AATATT 2 cut(s) 291, 625
SspMI CTAG 2 cut(s) 131, 591
StyD4I CCNGG 2 cut(s) 697, 799
TaaI ACNGT 1 cut(s) 688
TaiI ACGT 1 cut(s) 729
TaqI TCGA 4 cut(s) 135, 237, 754, 819
TasI AATT 6 cut(s) 68, 90, 168, 495, 806, 1021
TfiI GAWTC 4 cut(s) 114, 234, 490, 796
Tru1I TTAA 3 cut(s) 210, 867, 1024
Tru9I TTAA 3 cut(s) 210, 867, 1024
TscAI CASTG 2 cut(s) 196, 781
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 1 cut(s) 565
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 2 cut(s) 106, 635
TspGWI ACGGA 2 cut(s) 598, 986
TspRI CASTG 2 cut(s) 196, 781
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 2 cut(s) 611, 779
XapI RAATTY 3 cut(s) 68, 90, 806
XceI RCATGY 2 cut(s) 23, 382
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 2 cut(s) 131, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.