Rw1G032830

Protein EARLY RESPONSIVE TO DEHYDRATION 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0012654
Physical Location & Seq
Forward (+)
61069464 .. 61070008
545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032830.1

Sequence Viewer

Length: 453 bp
ATGGACGTCATTTCCCGGCAGCACCCGCAGTCGACTCTGAACCCGAACGCTCCGATGTTCGTTCCGTCGGCTTATCGGACGGTGGAGGACTTCTCCGCCGAGTGGTGGGCCCTGGTCCAGTCCTCCCCTTGGTTCCGAGACTACTGGCTCCAGGAGAACTTCCAGGATCCCCAGAATGACCCCTTCTTTTCCGATATTCCCGACGACTCCGACTACATCGACGACCTCTTCGACGAGGAACACTACCCCACCGCCACCAACAACAACAACGCACAACAGGTGGTGGAGGAGGAAGAAGAGGAGGAGAAAGACTATCACAAGGAGCTGGTTTCGTTGGGACTGTTGAAGTGGCCGAAGGGTCGAGCTGTGGCGAAGGCGCCGAGGTTTATAGAGAAAGCTCCGAAGATTGTGAACGTCAAAGTGAGTCCCAGGGCGATTCAGCAGCCGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAM2

Protein Analysis

150

Amino Acids

17.55

Weight (kDa)

4.57

Isoelectric Point (pI)

59.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 9
AccB1I GGYRCC 1 cut(s) 376
AccI GTMKAC 1 cut(s) 32
AciI CCGC 3 cut(s) 26, 96, 252
AclWI GGATC 2 cut(s) 161, 174
AcoI YGGCCR 1 cut(s) 350
AcyI GRCGYC 2 cut(s) 6, 377
AfiI CCNNNNNNNGG 3 cut(s) 102, 105, 129
AgsI TTSAA 1 cut(s) 346
AjnI CCWGG 4 cut(s) 111, 150, 162, 428
AluBI AGCT 3 cut(s) 325, 365, 398
AluI AGCT 3 cut(s) 325, 365, 398
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 2 cut(s) 161, 174
AoxI GGCC 2 cut(s) 108, 350
ApaI GGGCCC 1 cut(s) 112
ApeKI GCWGC 2 cut(s) 19, 442
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
AspLEI GCGC 1 cut(s) 379
AspS9I GGNCC 3 cut(s) 108, 109, 115
AsuC2I CCSGG 1 cut(s) 16
AvaII GGWCC 1 cut(s) 115
BaeGI GKGCMC 1 cut(s) 112
BamHI GGATCC 1 cut(s) 166
BanI GGYRCC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 112
BbvI GCAGC 1 cut(s) 31
BciT130I CCWGG 4 cut(s) 113, 152, 164, 430
BcnI CCSGG 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 132
BfoI RGCGCY 1 cut(s) 380
BisI GCNGC 2 cut(s) 20, 443
BlsI GCNGC 2 cut(s) 21, 444
Bme1390I CCNGG 5 cut(s) 16, 113, 152, 164, 430
Bme18I GGWCC 1 cut(s) 115
BmgT120I GGNCC 3 cut(s) 108, 109, 115
BmiI GGNNCC 5 cut(s) 110, 134, 149, 168, 378
BmrFI CCNGG 5 cut(s) 16, 113, 152, 164, 430
BplI GAGNNNNNCTC 2 cut(s) 77, 109
BpmI CTGGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 16
BsaHI GRCGYC 2 cut(s) 6, 377
BsaJI CCNNGG 6 cut(s) 111, 128, 380, 428, 429, 446
Bsc4I CCNNNNNNNGG 3 cut(s) 102, 105, 129
Bse1I ACTGG 2 cut(s) 118, 149
BseBI CCWGG 4 cut(s) 113, 152, 164, 430
BseDI CCNNGG 6 cut(s) 111, 128, 380, 428, 429, 446
BseLI CCNNNNNNNGG 3 cut(s) 102, 105, 129
BseNI ACTGG 2 cut(s) 118, 149
BseRI GAGGAG 3 cut(s) 302, 314, 317
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 1 cut(s) 31
BshFI GGCC 2 cut(s) 110, 352
BshNI GGYRCC 1 cut(s) 376
BsiSI CCGG 1 cut(s) 16
BslFI GGGAC 2 cut(s) 351, 411
BslI CCNNNNNNNGG 3 cut(s) 102, 105, 129
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 2 cut(s) 351, 411
BsnI GGCC 2 cut(s) 110, 352
Bsp120I GGGCCC 1 cut(s) 108
Bsp1286I GDGCHC 1 cut(s) 112
Bsp143I GATC 1 cut(s) 166
BspACI CCGC 3 cut(s) 26, 96, 252
BspANI GGCC 2 cut(s) 110, 352
BspLI GGNNCC 5 cut(s) 110, 134, 149, 168, 378
BspPI GGATC 2 cut(s) 161, 174
BspT107I GGYRCC 1 cut(s) 376
BsrI ACTGG 2 cut(s) 118, 149
BssECI CCNNGG 6 cut(s) 111, 128, 380, 428, 429, 446
BssMI GATC 1 cut(s) 166
BssNI GRCGYC 2 cut(s) 6, 377
BssT1I CCWWGG 1 cut(s) 128
Bst2UI CCWGG 4 cut(s) 113, 152, 164, 430
Bst4CI ACNGT 2 cut(s) 82, 342
Bst6I CTCTTC 2 cut(s) 233, 291
BstACI GRCGYC 2 cut(s) 6, 377
BstH2I RGCGCY 1 cut(s) 380
BstHHI GCGC 1 cut(s) 379
BstKTI GATC 1 cut(s) 169
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 1 cut(s) 166
BstMWI GCNNNNNNNGC 1 cut(s) 25
BstNI CCWGG 4 cut(s) 113, 152, 164, 430
BstSCI CCNGG 5 cut(s) 14, 111, 150, 162, 428
BstSLI GKGCMC 1 cut(s) 112
BstV1I GCAGC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BsuRI GGCC 2 cut(s) 110, 352
CfoI GCGC 1 cut(s) 379
Cfr13I GGNCC 3 cut(s) 108, 109, 115
CviJI RGCY 8 cut(s) 71, 110, 148, 325, 352, 365, 398, 445
CviKI_1 RGCY 8 cut(s) 71, 110, 148, 325, 352, 365, 398, 445
DinI GGCGCC 1 cut(s) 378
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
EaeI YGGCCR 1 cut(s) 350
Eam1104I CTCTTC 2 cut(s) 233, 291
EarI CTCTTC 2 cut(s) 233, 291
EciI GGCGGA 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 128
Eco24I GRGCYC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 115
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 4 cut(s) 111, 150, 162, 428
EcoT14I CCWWGG 1 cut(s) 128
EcoT38I GRGCYC 1 cut(s) 112
EgeI GGCGCC 1 cut(s) 378
EheI GGCGCC 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 128
FaiI YATR 1 cut(s) 389
FaqI GGGAC 2 cut(s) 351, 411
FauI CCCGC 1 cut(s) 33
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 2 cut(s) 20, 443
FriOI GRGCYC 1 cut(s) 112
Fsp4HI GCNGC 2 cut(s) 20, 443
GlaI GCGC 1 cut(s) 378
GluI GCNGC 2 cut(s) 20, 443
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 380
HaeIII GGCC 2 cut(s) 110, 352
HapII CCGG 1 cut(s) 16
HhaI GCGC 1 cut(s) 379
Hin1I GRCGYC 2 cut(s) 6, 377
Hin6I GCGC 1 cut(s) 377
HinP1I GCGC 1 cut(s) 377
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HinfI GANTC 4 cut(s) 34, 206, 424, 436
HpaII CCGG 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 33, 412
Hpy188I TCNGA 7 cut(s) 39, 54, 78, 137, 193, 211, 402
Hpy188III TCNNGA 1 cut(s) 200
Hpy8I GTNNAC 2 cut(s) 33, 412
Hpy99I CGWCG 4 cut(s) 70, 206, 224, 236
HpyAV CCTTC 3 cut(s) 193, 349, 367
HpyCH4III ACNGT 2 cut(s) 82, 342
HpyCH4IV ACGT 2 cut(s) 6, 414
HpyF10VI GCNNNNNNNGC 1 cut(s) 25
HpySE526I ACGT 2 cut(s) 6, 414
Hsp92I GRCGYC 2 cut(s) 6, 377
HspAI GCGC 1 cut(s) 377
KasI GGCGCC 1 cut(s) 376
Kzo9I GATC 1 cut(s) 166
LmnI GCTCC 4 cut(s) 55, 153, 322, 403
Lsp1109I GCAGC 1 cut(s) 31
MaeII ACGT 2 cut(s) 6, 414
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 4 cut(s) 220, 305, 308, 415
MflI RGATCY 1 cut(s) 166
MhlI GDGCHC 1 cut(s) 112
Mly113I GGCGCC 1 cut(s) 377
MlyI GAGTC 3 cut(s) 28, 200, 433
MmeI TCCRAC 1 cut(s) 234
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 5 cut(s) 16, 113, 152, 164, 430
MvaI CCWGG 4 cut(s) 113, 152, 164, 430
MwoI GCNNNNNNNGC 1 cut(s) 25
NarI GGCGCC 1 cut(s) 377
NciI CCSGG 1 cut(s) 16
NdeII GATC 1 cut(s) 166
NlaIV GGNNCC 5 cut(s) 110, 134, 149, 168, 378
NmeAIII GCCGAG 2 cut(s) 124, 405
PasI CCCWGGG 1 cut(s) 429
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PfeI GAWTC 1 cut(s) 436
PfoI TCCNGGA 2 cut(s) 150, 162
PkrI GCNGC 2 cut(s) 21, 444
PleI GAGTC 3 cut(s) 28, 200, 432
PluTI GGCGCC 1 cut(s) 380
PpsI GAGTC 3 cut(s) 28, 200, 432
Psp6I CCWGG 4 cut(s) 111, 150, 162, 428
PspGI CCWGG 4 cut(s) 111, 150, 162, 428
PspN4I GGNNCC 5 cut(s) 110, 134, 149, 168, 378
PspOMI GGGCCC 1 cut(s) 108
PspPI GGNCC 3 cut(s) 108, 109, 115
PsuI RGATCY 1 cut(s) 166
SalI GTCGAC 1 cut(s) 31
SatI GCNGC 2 cut(s) 20, 443
Sau3AI GATC 1 cut(s) 166
Sau96I GGNCC 3 cut(s) 108, 109, 115
SchI GAGTC 3 cut(s) 28, 200, 433
ScrFI CCNGG 5 cut(s) 16, 113, 152, 164, 430
SduI GDGCHC 1 cut(s) 112
SetI ASST 9 cut(s) 9, 228, 282, 327, 367, 386, 400, 417, 452
SfoI GGCGCC 1 cut(s) 378
SinI GGWCC 1 cut(s) 115
SsiI CCGC 3 cut(s) 26, 96, 252
SspDI GGCGCC 1 cut(s) 376
StyD4I CCNGG 5 cut(s) 14, 111, 150, 162, 428
StyI CCWWGG 1 cut(s) 128
TaaI ACNGT 2 cut(s) 82, 342
TaiI ACGT 2 cut(s) 9, 417
TaqI TCGA 4 cut(s) 32, 219, 231, 361
TfiI GAWTC 1 cut(s) 436
TseI GCWGC 2 cut(s) 19, 442
TspGWI ACGGA 1 cut(s) 54
VpaK11BI GGWCC 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 32
ZraI GACGTC 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.