Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0007543

Physical Location & Seq

Genomic Coordinates Forward (+)

4526556 .. 4529463

Gene Structure 2908 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0058500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGGCAGCCTTACACATCAAGCTTTTTTGCATCCTTCCTCTCCTCTTCGCCCTTGGCATCAATGCACAATGGCGAGGGCTTCCTTTTATCCCCAATCCGTTAGTTTCACACCACGGACACCACGGATTGGGTTTTAATCTTGACCCCAGCAACGGGGGCCAATTTCAGTTCTTTGACCCCGAGCCCGTCACTTTCAAAGCAGCCGGCGATAAACCGAAACCCAACGCCGGGGGTAAACCCAAACCCATCGCCGGGGGTGAACCCCAACCCATCGCCGGGGGTGAACCCCAACCCATCGCCGGGGGTGAACCCCAACCCATCGCCGGGGGTAAACCCCAACCCGACGCAGGGGGTAAACCCAACGTCGGGGGCATACCCAATCCAGCGGTCATTAAAGCCGAAGATGTTAATACTGTTCCCAACGCACAACCACAGCCGAACTTCCCTATTGGCAATAACCCGGGGGCGTGGGCACTTGTTTCCAATGACGCCGGTGTGTCTGCCATGCATCTAAATGTGCTTCCCAACAACAAGGCCATAATGTACGATGCCTCGGATTTCCATATATCGAACATCAAATTGCCCAACAATGAATGCCTTCCCTGGAAAAATAATAAGGGCGATGCAGGAAACGATTGTTTTGCTCATGCAGTTGAATATGATCTTGACAAAAACACAGTTAGACCACTCAAGGTTCAGTTTGATGCATGGTGCTCATCGGGAGGGATTTTGCCCGATGGTAGGCTCATCAGCACCGGTGGTTGGGTGAACGGATATAGAAGCGCTAGATATATAACCCCATGCAACGACGGCAAATGCGACTTCAAAGAGTACCAAAACGCATTTGCAGAAAATAGATGGTATGCAACTCAGATAACGCTAGCAGACGGTAGGGTGATTGTGGTTGGTGGCCGGAAAGCATACAGCATCGAGTACGTGCCACCAGAGGGACAAGAAAACAAGCAATCCATCTTCCTGCCTTTCCTTGACGAGACCACCGACATGGACGAGAACAATCTCTACCCGTTCGTCCACCAATCTACCGATGGCAACGTCTTCATCTTTGCCAACGACCGCTCCGTCCTCCTCGATCCCAAAACTAACAAAGTCATCAAGGAGTTCCCCAAACTCGACGGCGGATCCCGAAACTACCCGGCCTCCGGCATGTCGGCACTGCTCCCCATCGAGCTCAATGAGCAGAACCCCCCGGTCATCCCTGTCGACGTCCTCGTCTGCGGTGGCAATAAACCCGACGCTTTCAAAATGTCCGCACAGAAACCACCAGTCTTCGTCCCTGCCTTGGACGACTGCGGACGCCTCCGGATCACCGACCCTAAACCGGTCTGGGAAAAGGAGGTCATGCCCTCAAGACGTGTCATGGGAGACATGCTAAACCTTCCCACAGGTGAGCTCTTGATGATAAACGGTGCGATGGCTGGTGCCTCAGCTTGGTGGCAAGCTGAAGAACCCAACTTTACCCCTGTGATGTACAACCCCAAATTGCCCAATGGACAACGGTTCGTCAAGATGGCACCGACAACTATTGCAAGAATGTACCACTCGACCTCGGCAGTGCTCCCTAACGGTAAAATCCTCGTGGCAGGCAGTAATACTAACCCGGGCTACGACTTCAAGGCCAAGTACCCAACTGAGGTACGGGTGGAGACCTTCACCCCACCTTACATGGATAAGGCTTTGGACATCCACAGGCCGGAAATCAACGCCCAGGCCTCCGACAACAAGCTCAAGTACGGCGCACCGTTCGGCGTCCAGTTCAAGCTGAACGAGCAAGATAAGCCTACAAAGGCTGATATCAAGGTCACCGTGTACGCTGTTCCTTTCACAACACATGGCTACTCCATGAACCAGAGGCTTGTTGTTTTGGCGAACACTGAGCTCAAGGCAGAGGGAGCTGGCTTATATCGTGTCACGGCGTTGGCCCCACCAACCGGAGCGGTGGCGCCACCAGGGTCCTACCTGCTGAATGTGGTTCACCGCGGAGTGCCGAGCACGGGAGTGTGGGTGACGATTAACCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NTP_transf_2 PAP_assoc

Protein Analysis

679

Amino Acids

73.64

Weight (kDa)

6.68

Isoelectric Point (pI)

39.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyoxal_oxid_N	PF07250	169 - 561	1.4e-162	Glyoxal oxidase N-terminus
GO-like_E_set	PF09118	570 - 677	7.9e-26	Galactose oxidase-like, Early set domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007543)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr1g0316831 RchiOBHm_Chr2g0167831 RchiOBHm_Chr2g0167841 RchiOBHm_Chr5g0049311 RchiOBHm_Chr5g0049321 RchiOBHm_Chr7g0229001
rosa_laevigata	RLG00000030641
rosa_multiflora	Rmu_sc0006695.1_g000030 Rmu_sc0006695.1_g000031 Rmu_ssc0000059.1_g000008
rosa_roxburghii	Rroxscaffold_7G00194280
rosa_rugosa	Rorug02G0058500 Rorug02G0175900 Rorug03G0198300 Rorug04G0115200
rosa_samantha	Rh1AG024200 Rh1AG198900 Rh1BG003300 Rh1CG011000 Rh1DG021200 Rh2BG611900 Rh2CG283900 Rh5BG335100 Rh5DG347400
rosa_wichuraiana	Rw1G001640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1079, 1229
AatII	GACGTC	1 cut(s)	1227
Acc36I	ACCTGC	1 cut(s)	1986
AccB1I	GGYRCC	3 cut(s)	1439, 1531, 1960
AccB7I	CCANNNNNTGG	1 cut(s)	127
AccBSI	CCGCTC	2 cut(s)	1077, 1955
AccI	GTMKAC	1 cut(s)	1221
AccII	CGCG	1 cut(s)	1998
AccIII	TCCGGA	1 cut(s)	1320
AciI	CCGC	9 cut(s)	386, 1075, 1137, 1236, 1269, 1311, 1955, 1996, 1998
AclWI	GGATC	4 cut(s)	1085, 1134, 1147, 1331
AcoI	YGGCCR	1 cut(s)	910
AcuI	CTGAAG	1 cut(s)	1482
AcyI	GRCGYC	5 cut(s)	489, 1224, 1315, 1767, 1961
AfaI	GTAC	9 cut(s)	545, 833, 935, 1490, 1556, 1643, 1656, 1751, 1829
AfeI	AGCGCT	1 cut(s)	784
AflIII	ACRYGT	1 cut(s)	1372
AgeI	ACCGGT	2 cut(s)	755, 1339
AgsI	TTSAA	6 cut(s)	196, 656, 826, 1261, 1633, 1777
AjiI	CACGTC	1 cut(s)	1373
AjnI	CCWGG	3 cut(s)	602, 1725, 1966
AleI	CACNNNNGTG	1 cut(s)	2015
AluBI	AGCT	9 cut(s)	22, 1189, 1411, 1448, 1460, 1744, 1780, 1897, 1913
AluI	AGCT	9 cut(s)	22, 1189, 1411, 1448, 1460, 1744, 1780, 1897, 1913
Alw21I	GWGCWC	6 cut(s)	716, 1191, 1413, 1578, 1899, 2012
Alw26I	GTCTC	3 cut(s)	986, 1377, 1658
AlwI	GGATC	4 cut(s)	1085, 1134, 1147, 1331
Ama87I	CYCGRG	3 cut(s)	179, 460, 1618
Aor13HI	TCCGGA	1 cut(s)	1320
Aor51HI	AGCGCT	1 cut(s)	784
AoxI	GGCC	8 cut(s)	157, 534, 910, 1155, 1635, 1709, 1728, 1938
ApeKI	GCWGC	2 cut(s)	5, 200
AsiGI	ACCGGT	2 cut(s)	755, 1339
Asp700I	GAANNNNTTC	1 cut(s)	597
AspLEI	GCGC	3 cut(s)	785, 1757, 1963
AspS9I	GGNCC	3 cut(s)	157, 1939, 1971
AsuNHI	GCTAGC	1 cut(s)	880
AvaI	CYCGRG	3 cut(s)	179, 460, 1618
AvaII	GGWCC	1 cut(s)	1971
BaeGI	GKGCMC	1 cut(s)	475
BamHI	GGATCC	1 cut(s)	1139
BanI	GGYRCC	3 cut(s)	1439, 1531, 1960
BanII	GRGCYC	4 cut(s)	186, 1191, 1413, 1899
BauI	CACGAG	1 cut(s)	1595
BbsI	GAAGAC	2 cut(s)	1048, 1279
Bbv12I	GWGCWC	6 cut(s)	716, 1191, 1413, 1578, 1899, 2012
BbvCI	CCTCAGC	1 cut(s)	1444
BbvI	GCAGC	2 cut(s)	17, 212
BceAI	ACGGC	4 cut(s)	826, 1150, 1768, 1947
BcgI	CGANNNNNNTGC	2 cut(s)	623, 657
BciT130I	CCWGG	3 cut(s)	604, 1727, 1968
BcoDI	GTCTC	3 cut(s)	986, 1377, 1658
BfaI	CTAG	2 cut(s)	786, 881
BfoI	RGCGCY	2 cut(s)	786, 1964
BfuAI	ACCTGC	1 cut(s)	1986
BisI	GCNGC	2 cut(s)	6, 201
BlsI	GCNGC	2 cut(s)	7, 202
Bme18I	GGWCC	1 cut(s)	1971
BmeT110I	CYCGRG	3 cut(s)	179, 460, 1618
BmgBI	CACGTC	1 cut(s)	1373
BmgT120I	GGNCC	3 cut(s)	157, 1939, 1971
BmiI	GGNNCC	7 cut(s)	158, 1141, 1441, 1533, 1941, 1962, 1972
BmsI	GCATC	7 cut(s)	39, 66, 517, 538, 613, 694, 936
BmtI	GCTAGC	1 cut(s)	884
BpiI	GAAGAC	2 cut(s)	1048, 1279
Bpu10I	CCTNAGC	1 cut(s)	1444
BpuEI	CTTGAG	4 cut(s)	674, 1351, 1730, 1883
BsaAI	YACGTR	1 cut(s)	937
BsaHI	GRCGYC	5 cut(s)	489, 1224, 1315, 1767, 1961
BsaI	GGTCTC	2 cut(s)	986, 1658
BsaWI	WCCGGW	4 cut(s)	755, 1320, 1339, 1949
BsaXI	ACNNNNNCTCC	6 cut(s)	1061, 1091, 1142, 1172, 2007, 2037
Bse118I	RCCGGY	4 cut(s)	203, 491, 755, 1339
Bse1I	ACTGG	3 cut(s)	1283, 1771, 2035
BseAI	TCCGGA	1 cut(s)	1320
BseBI	CCWGG	3 cut(s)	604, 1727, 1968
BseGI	GGATG	3 cut(s)	30, 1212, 1701
BseMII	CTCAG	4 cut(s)	884, 1458, 1641, 1884
BseNI	ACTGG	3 cut(s)	1283, 1771, 2035
BseRI	GAGGAG	2 cut(s)	32, 1076
BseSI	GKGCMC	1 cut(s)	475
BseXI	GCAGC	2 cut(s)	17, 212
BseYI	CCCAGC	1 cut(s)	146
Bsh1236I	CGCG	1 cut(s)	1998
Bsh1285I	CGRYCG	1 cut(s)	1075
BshFI	GGCC	8 cut(s)	159, 536, 912, 1157, 1637, 1711, 1730, 1940
BshNI	GGYRCC	3 cut(s)	1439, 1531, 1960
BshTI	ACCGGT	2 cut(s)	755, 1339
BsiEI	CGRYCG	1 cut(s)	1075
BsiHKAI	GWGCWC	6 cut(s)	716, 1191, 1413, 1578, 1899, 2012
BsiHKCI	CYCGRG	3 cut(s)	179, 460, 1618
BslFI	GGGAC	2 cut(s)	963, 1277
BsmAI	GTCTC	3 cut(s)	986, 1377, 1658
BsmFI	GGGAC	2 cut(s)	963, 1277
BsmI	GAATGC	1 cut(s)	599
BsnI	GGCC	8 cut(s)	159, 536, 912, 1157, 1637, 1711, 1730, 1940
Bso31I	GGTCTC	2 cut(s)	986, 1658
BsoBI	CYCGRG	3 cut(s)	179, 460, 1618
Bsp1286I	GDGCHC	8 cut(s)	186, 475, 716, 1191, 1413, 1578, 1899, 2012
Bsp13I	TCCGGA	1 cut(s)	1320
Bsp1407I	TGTACA	1 cut(s)	1488
Bsp143I	GATC	4 cut(s)	661, 1090, 1139, 1323
BspACI	CCGC	9 cut(s)	386, 1075, 1137, 1236, 1269, 1311, 1955, 1996, 1998
BspANI	GGCC	8 cut(s)	159, 536, 912, 1157, 1637, 1711, 1730, 1940
BspCNI	CTCAG	4 cut(s)	883, 1457, 1642, 1885
BspEI	TCCGGA	1 cut(s)	1320
BspFNI	CGCG	1 cut(s)	1998
BspLI	GGNNCC	7 cut(s)	158, 1141, 1441, 1533, 1941, 1962, 1972
BspMI	ACCTGC	1 cut(s)	1986
BspOI	GCTAGC	1 cut(s)	884
BspPI	GGATC	4 cut(s)	1085, 1134, 1147, 1331
BspT107I	GGYRCC	3 cut(s)	1439, 1531, 1960
BspTNI	GGTCTC	2 cut(s)	986, 1658
BsrBI	CCGCTC	2 cut(s)	1077, 1955
BsrFI	RCCGGY	4 cut(s)	203, 491, 755, 1339
BsrGI	TGTACA	1 cut(s)	1488
BsrI	ACTGG	3 cut(s)	1283, 1771, 2035
BssAI	RCCGGY	4 cut(s)	203, 491, 755, 1339
BssMI	GATC	4 cut(s)	661, 1090, 1139, 1323
BssNI	GRCGYC	5 cut(s)	489, 1224, 1315, 1767, 1961
BssSI	CACGAG	1 cut(s)	1595
BssT1I	CCWWGG	2 cut(s)	52, 1299
Bst2BI	CACGAG	1 cut(s)	1595
Bst2UI	CCWGG	3 cut(s)	604, 1727, 1968
Bst4CI	ACNGT	8 cut(s)	415, 679, 890, 1427, 1518, 1586, 1761, 1825
Bst6I	CTCTTC	1 cut(s)	50
BstACI	GRCGYC	5 cut(s)	489, 1224, 1315, 1767, 1961
BstAUI	TGTACA	1 cut(s)	1488
BstBAI	YACGTR	1 cut(s)	937
BstC8I	GCNNGC	5 cut(s)	205, 882, 1458, 1603, 1915
BstDEI	CTNAG	4 cut(s)	870, 1444, 1650, 1893
BstDSI	CCRYGG	3 cut(s)	112, 121, 1996
BstEII	GGTNACC	1 cut(s)	1819
BstF5I	GGATG	3 cut(s)	30, 1212, 1701
BstFNI	CGCG	1 cut(s)	1998
BstH2I	RGCGCY	2 cut(s)	786, 1964
BstHHI	GCGC	3 cut(s)	785, 1757, 1963
BstKTI	GATC	4 cut(s)	664, 1093, 1142, 1326
BstMAI	GTCTC	3 cut(s)	986, 1377, 1658
BstMBI	GATC	4 cut(s)	661, 1090, 1139, 1323
BstMCI	CGRYCG	1 cut(s)	1075
BstMWI	GCNNNNNNNGC	6 cut(s)	156, 810, 1195, 1786, 1795, 1910
BstNI	CCWGG	3 cut(s)	604, 1727, 1968
BstNSI	RCATGY	2 cut(s)	1168, 1390
BstPI	GGTNACC	1 cut(s)	1819
BstSLI	GKGCMC	1 cut(s)	475
BstUI	CGCG	1 cut(s)	1998
BstV1I	GCAGC	2 cut(s)	17, 212
BstV2I	GAAGAC	2 cut(s)	1048, 1279
BstX2I	RGATCY	1 cut(s)	1139
BstXI	CCANNNNNNTGG	1 cut(s)	1003
BstYI	RGATCY	1 cut(s)	1139
BsuRI	GGCC	8 cut(s)	159, 536, 912, 1157, 1637, 1711, 1730, 1940
BtgI	CCRYGG	3 cut(s)	112, 121, 1996
BtgZI	GCGATG	6 cut(s)	232, 256, 280, 304, 636, 1445
BtrI	CACGTC	1 cut(s)	1373
BtsCI	GGATG	3 cut(s)	30, 1212, 1701
BtsI	GCAGTG	2 cut(s)	1172, 1578
BtsIMutI	CAGTG	3 cut(s)	1172, 1578, 1890
BveI	ACCTGC	1 cut(s)	1986
Cac8I	GCNNGC	5 cut(s)	205, 882, 1458, 1603, 1915
CfoI	GCGC	3 cut(s)	785, 1757, 1963
Cfr10I	RCCGGY	4 cut(s)	203, 491, 755, 1339
Cfr13I	GGNCC	3 cut(s)	157, 1939, 1971
Cfr42I	CCGCGG	1 cut(s)	1999
Cfr9I	CCCGGG	2 cut(s)	460, 1618
CseI	GACGC	5 cut(s)	353, 497, 1262, 1323, 1756
Csp6I	GTAC	9 cut(s)	544, 832, 934, 1489, 1555, 1642, 1655, 1750, 1828
CspAI	ACCGGT	2 cut(s)	755, 1339
CviQI	GTAC	9 cut(s)	544, 832, 934, 1489, 1555, 1642, 1655, 1750, 1828
DdeI	CTNAG	4 cut(s)	870, 1444, 1650, 1893
DinI	GGCGCC	1 cut(s)	1962
DpnI	GATC	4 cut(s)	663, 1092, 1141, 1325
DpnII	GATC	4 cut(s)	661, 1090, 1139, 1323
DrdI	GACNNNNNNGTC	2 cut(s)	1079, 1229
DseDI	GACNNNNNNGTC	2 cut(s)	1079, 1229
EaeI	YGGCCR	1 cut(s)	910
Eam1104I	CTCTTC	1 cut(s)	50
EarI	CTCTTC	1 cut(s)	50
EciI	GGCGGA	1 cut(s)	1152
Ecl136II	GAGCTC	3 cut(s)	1189, 1411, 1897
Eco130I	CCWWGG	2 cut(s)	52, 1299
Eco147I	AGGCCT	1 cut(s)	1730
Eco24I	GRGCYC	4 cut(s)	186, 1191, 1413, 1899
Eco31I	GGTCTC	2 cut(s)	986, 1658
Eco32I	GATATC	1 cut(s)	1813
Eco47I	GGWCC	1 cut(s)	1971
Eco47III	AGCGCT	1 cut(s)	784
Eco53kI	GAGCTC	3 cut(s)	1189, 1411, 1897
Eco57I	CTGAAG	1 cut(s)	1482
Eco88I	CYCGRG	3 cut(s)	179, 460, 1618
Eco91I	GGTNACC	1 cut(s)	1819
EcoICRI	GAGCTC	3 cut(s)	1189, 1411, 1897
EcoO109I	RGGNCCY	1 cut(s)	1971
EcoO65I	GGTNACC	1 cut(s)	1819
EcoRII	CCWGG	3 cut(s)	602, 1725, 1966
EcoRV	GATATC	1 cut(s)	1813
EcoT14I	CCWWGG	2 cut(s)	52, 1299
EcoT22I	ATGCAT	2 cut(s)	510, 709
EcoT38I	GRGCYC	4 cut(s)	186, 1191, 1413, 1899
EgeI	GGCGCC	1 cut(s)	1962
EheI	GGCGCC	1 cut(s)	1962
ErhI	CCWWGG	2 cut(s)	52, 1299
FaqI	GGGAC	2 cut(s)	963, 1277
FblI	GTMKAC	1 cut(s)	1221
Fnu4HI	GCNGC	2 cut(s)	6, 201
FokI	GGATG	3 cut(s)	17, 1199, 1688
FriOI	GRGCYC	4 cut(s)	186, 1191, 1413, 1899
Fsp4HI	GCNGC	2 cut(s)	6, 201
FspBI	CTAG	2 cut(s)	786, 881
GlaI	GCGC	3 cut(s)	784, 1756, 1962
GluI	GCNGC	2 cut(s)	6, 201
GsaI	CCCAGC	1 cut(s)	150
HaeII	RGCGCY	2 cut(s)	786, 1964
HaeIII	GGCC	8 cut(s)	159, 536, 912, 1157, 1637, 1711, 1730, 1940
HgaI	GACGC	5 cut(s)	353, 497, 1262, 1323, 1756
HhaI	GCGC	3 cut(s)	785, 1757, 1963
Hin1I	GRCGYC	5 cut(s)	489, 1224, 1315, 1767, 1961
Hin6I	GCGC	3 cut(s)	783, 1755, 1961
HinP1I	GCGC	3 cut(s)	783, 1755, 1961
HincII	GTYRAC	1 cut(s)	1222
HindII	GTYRAC	1 cut(s)	1222
HindIII	AAGCTT	1 cut(s)	20
Hpy188I	TCNGA	3 cut(s)	556, 873, 1735
Hpy188III	TCNNGA	8 cut(s)	140, 665, 720, 1143, 1321, 1368, 1414, 1525
Hpy99I	CGWCG	6 cut(s)	347, 368, 812, 1136, 1226, 1256
HpyAV	CCTTC	4 cut(s)	44, 608, 1406, 1678
HpyCH4III	ACNGT	8 cut(s)	415, 679, 890, 1427, 1518, 1586, 1761, 1825
HpyCH4IV	ACGT	5 cut(s)	363, 936, 1053, 1224, 1372
HpyF10VI	GCNNNNNNNGC	6 cut(s)	156, 810, 1195, 1786, 1795, 1910
HpyF3I	CTNAG	4 cut(s)	870, 1444, 1650, 1893
HpySE526I	ACGT	5 cut(s)	363, 936, 1053, 1224, 1372
Hsp92I	GRCGYC	5 cut(s)	489, 1224, 1315, 1767, 1961
HspAI	GCGC	3 cut(s)	783, 1755, 1961
KasI	GGCGCC	1 cut(s)	1960
Kpn2I	TCCGGA	1 cut(s)	1320
KroI	GCCGGC	1 cut(s)	203
KroNI	GCCGGC	1 cut(s)	205
KspI	CCGCGG	1 cut(s)	1999
Kzo9I	GATC	4 cut(s)	661, 1090, 1139, 1323
LmnI	GCTCC	5 cut(s)	1082, 1182, 1581, 1910, 1952
Lsp1109I	GCAGC	2 cut(s)	17, 212
LweI	GCATC	7 cut(s)	39, 66, 517, 538, 613, 694, 936
MaeI	CTAG	2 cut(s)	786, 881
MaeII	ACGT	5 cut(s)	363, 936, 1053, 1224, 1372
MaeIII	GTNAC	4 cut(s)	187, 1819, 1927, 2023
MalI	GATC	4 cut(s)	663, 1092, 1141, 1325
MbiI	CCGCTC	2 cut(s)	1077, 1955
MboI	GATC	4 cut(s)	661, 1090, 1139, 1323
MboII	GAAGA	6 cut(s)	37, 413, 964, 1048, 1279, 1475
MflI	RGATCY	1 cut(s)	1139
MhlI	GDGCHC	8 cut(s)	186, 475, 716, 1191, 1413, 1578, 1899, 2012
MluCI	AATT	3 cut(s)	161, 578, 1499
Mly113I	GGCGCC	1 cut(s)	1961
MmeI	TCCRAC	1 cut(s)	1758
Mph1103I	ATGCAT	2 cut(s)	510, 709
MroI	TCCGGA	1 cut(s)	1320
MroNI	GCCGGC	1 cut(s)	203
MroXI	GAANNNNTTC	1 cut(s)	597
MseI	TTAA	4 cut(s)	135, 393, 408, 2031
MslI	CAYNNNNRTG	3 cut(s)	513, 1001, 2015
MspA1I	CMGCKG	2 cut(s)	386, 1998
Mva1269I	GAATGC	1 cut(s)	599
MvaI	CCWGG	3 cut(s)	604, 1727, 1968
MvnI	CGCG	1 cut(s)	1998
MwoI	GCNNNNNNNGC	6 cut(s)	156, 810, 1195, 1786, 1795, 1910
NaeI	GCCGGC	1 cut(s)	205
NarI	GGCGCC	1 cut(s)	1961
NdeII	GATC	4 cut(s)	661, 1090, 1139, 1323
NgoMIV	GCCGGC	1 cut(s)	203
NheI	GCTAGC	1 cut(s)	880
NlaIV	GGNNCC	7 cut(s)	158, 1141, 1441, 1533, 1941, 1962, 1972
NmeAIII	GCCGAG	2 cut(s)	1547, 2031
NmuCI	GTSAC	4 cut(s)	187, 1819, 1927, 2023
NsiI	ATGCAT	2 cut(s)	510, 709
NspI	RCATGY	2 cut(s)	1168, 1390
OliI	CACNNNNGTG	1 cut(s)	2015
PceI	AGGCCT	1 cut(s)	1730
PcsI	WCGNNNNNNNCGW	4 cut(s)	816, 996, 1077, 1227
PctI	GAATGC	1 cut(s)	599
PdiI	GCCGGC	1 cut(s)	205
PdmI	GAANNNNTTC	1 cut(s)	597
PflMI	CCANNNNNTGG	1 cut(s)	127
PinAI	ACCGGT	2 cut(s)	755, 1339
PkrI	GCNGC	2 cut(s)	7, 202
PluTI	GGCGCC	1 cut(s)	1964
Ppu21I	YACGTR	1 cut(s)	937
PpuMI	RGGWCCY	1 cut(s)	1971
Psp124BI	GAGCTC	3 cut(s)	1191, 1413, 1899
Psp5II	RGGWCCY	1 cut(s)	1971
Psp6I	CCWGG	3 cut(s)	602, 1725, 1966
PspEI	GGTNACC	1 cut(s)	1819
PspFI	CCCAGC	1 cut(s)	146
PspGI	CCWGG	3 cut(s)	602, 1725, 1966
PspN4I	GGNNCC	7 cut(s)	158, 1141, 1441, 1533, 1941, 1962, 1972
PspPI	GGNCC	3 cut(s)	157, 1939, 1971
PspPPI	RGGWCCY	1 cut(s)	1971
PsrI	GAACNNNNNNTAC	2 cut(s)	1004, 1036
PsuI	RGATCY	1 cut(s)	1139
RsaI	GTAC	9 cut(s)	545, 833, 935, 1490, 1556, 1643, 1656, 1751, 1829
RsaNI	GTAC	9 cut(s)	544, 832, 934, 1489, 1555, 1642, 1655, 1750, 1828
RseI	CAYNNNNRTG	3 cut(s)	513, 1001, 2015
SacI	GAGCTC	3 cut(s)	1191, 1413, 1899
SacII	CCGCGG	1 cut(s)	1999
SalI	GTCGAC	1 cut(s)	1220
SaqAI	TTAA	4 cut(s)	135, 393, 408, 2031
SatI	GCNGC	2 cut(s)	6, 201
Sau3AI	GATC	4 cut(s)	661, 1090, 1139, 1323
Sau96I	GGNCC	3 cut(s)	157, 1939, 1971
SduI	GDGCHC	8 cut(s)	186, 475, 716, 1191, 1413, 1578, 1899, 2012
SfaNI	GCATC	7 cut(s)	39, 66, 517, 538, 613, 694, 936
SfoI	GGCGCC	1 cut(s)	1962
Sfr303I	CCGCGG	1 cut(s)	1999
SgrAI	CRCCGGYG	2 cut(s)	491, 755
SgrBI	CCGCGG	1 cut(s)	1999
SinI	GGWCC	1 cut(s)	1971
SmaI	CCCGGG	2 cut(s)	462, 1620
SmiMI	CAYNNNNRTG	3 cut(s)	513, 1001, 2015
SmlI	CTYRAG	4 cut(s)	689, 1366, 1745, 1898
SmoI	CTYRAG	4 cut(s)	689, 1366, 1745, 1898
Sse9I	AATT	3 cut(s)	161, 578, 1499
SseBI	AGGCCT	1 cut(s)	1730
SsiI	CCGC	9 cut(s)	386, 1075, 1137, 1236, 1269, 1311, 1955, 1996, 1998
SspDI	GGCGCC	1 cut(s)	1960
SspMI	CTAG	2 cut(s)	786, 881
SstI	GAGCTC	3 cut(s)	1191, 1413, 1899
StuI	AGGCCT	1 cut(s)	1730
StyI	CCWWGG	2 cut(s)	52, 1299
TaaI	ACNGT	8 cut(s)	415, 679, 890, 1427, 1518, 1586, 1761, 1825
TaiI	ACGT	5 cut(s)	366, 939, 1056, 1227, 1375
TaqI	TCGA	7 cut(s)	569, 930, 1089, 1131, 1185, 1221, 1562
TasI	AATT	3 cut(s)	161, 578, 1499
TatI	WGTACW	1 cut(s)	1488
Tru1I	TTAA	4 cut(s)	135, 393, 408, 2031
Tru9I	TTAA	4 cut(s)	135, 393, 408, 2031
TscAI	CASTG	3 cut(s)	1179, 1578, 1897
TseFI	GTSAC	4 cut(s)	187, 1819, 1927, 2023
TseI	GCWGC	2 cut(s)	5, 200
Tsp45I	GTSAC	4 cut(s)	187, 1819, 1927, 2023
TspDTI	ATGAA	3 cut(s)	606, 1048, 1877
TspGWI	ACGGA	5 cut(s)	87, 129, 138, 786, 1069
TspMI	CCCGGG	2 cut(s)	460, 1618
TspRI	CASTG	3 cut(s)	1179, 1578, 1897
Van91I	CCANNNNNTGG	1 cut(s)	127
VpaK11BI	GGWCC	1 cut(s)	1971
XceI	RCATGY	2 cut(s)	1168, 1390
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1043
XmaI	CCCGGG	2 cut(s)	460, 1618
XmiI	GTMKAC	1 cut(s)	1221
XmnI	GAANNNNTTC	1 cut(s)	597
XspI	CTAG	2 cut(s)	786, 881
ZraI	GACGTC	1 cut(s)	1225
Zsp2I	ATGCAT	2 cut(s)	510, 709

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.