Rorug02G0101100

CRAL-TRIO domain-containing protein C23B6.04c-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007647
Physical Location & Seq
Forward (+)
8586846 .. 8587499
654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0101100.1

Sequence Viewer

Length: 456 bp
ATGTGTGAGGCTATGATTCTTGGGTCTCAGAAATTTTACTGCCCCTTTAAGGACTGCTCAGCTATGTTGATTGACGATGGGGCAGAGGTTGTGAGGGATTCGGAGTGCCCTAATTGCAATAGAATGTTCTGTGCTCAGTGTAAGGTTCCATGGCACGCCGGGATTGAGTGTGCGGAGTTTAAGAAGCTGAAGAATGATGAGAAGGGAAATGAGGACATCATGCTAAGGAACCTTGCGCAGAAGAACCAATGGAGGAGGTGTCCAAAGTGTAGGTTCTATGTGGAAAAATTGATAGGTTGCGATACCATGCGGTGCAGGTGCGGTACTTATTTCTGCTACATTTGTGGAAAACTTAATGTCACCTGCAGTTGTCAGCGCAGAAATTTAGATCGTGGAATGTTCTCTGGTGGATTTTACGGAAGGGGAAGAGTGGGGGTCGGATATTACAGGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

151

Amino Acids

17.48

Weight (kDa)

8.91

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBR PF01485 10 - 57 1.1e-09 IBR domain, a half RING-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 306, 371
Acc16I TGCGCA 1 cut(s) 237
Acc36I ACCTGC 2 cut(s) 306, 371
AciI CCGC 3 cut(s) 173, 310, 321
AcsI RAATTY 2 cut(s) 32, 382
AcuI CTGAAG 1 cut(s) 209
AfaI GTAC 1 cut(s) 325
AfiI CCNNNNNNNGG 1 cut(s) 49
AluBI AGCT 2 cut(s) 62, 187
AluI AGCT 2 cut(s) 62, 187
Alw21I GWGCWC 1 cut(s) 136
Alw26I GTCTC 1 cut(s) 30
ApoI RAATTY 2 cut(s) 32, 382
AspLEI GCGC 2 cut(s) 238, 378
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 352
BaeGI GKGCMC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 136
BccI CCATC 1 cut(s) 71
BcnI CCSGG 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 30
BfmI CTRYAG 1 cut(s) 364
BfuAI ACCTGC 2 cut(s) 306, 371
BlpI GCTNAGC 1 cut(s) 58
Bme1390I CCNGG 1 cut(s) 160
BmiI GGNNCC 2 cut(s) 147, 230
BmrFI CCNGG 1 cut(s) 160
Bpu10I CCTNAGC 1 cut(s) 224
Bpu1102I GCTNAGC 1 cut(s) 58
BpuMI CCSGG 1 cut(s) 160
BsaI GGTCTC 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 49
BseDI CCNNGG 1 cut(s) 149
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMII CTCAG 3 cut(s) 41, 72, 149
BseRI GAGGAG 1 cut(s) 268
BseSI GKGCMC 1 cut(s) 110
BsgI GTGCAG 1 cut(s) 334
BsiHKAI GWGCWC 1 cut(s) 136
BsiSI CCGG 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 1 cut(s) 30
Bso31I GGTCTC 1 cut(s) 30
Bsp1286I GDGCHC 2 cut(s) 110, 136
Bsp143I GATC 1 cut(s) 388
Bsp1720I GCTNAGC 1 cut(s) 58
Bsp19I CCATGG 1 cut(s) 149
BspACI CCGC 3 cut(s) 173, 310, 321
BspCNI CTCAG 3 cut(s) 40, 71, 148
BspLI GGNNCC 2 cut(s) 147, 230
BspMAI CTGCAG 1 cut(s) 368
BspMI ACCTGC 2 cut(s) 306, 371
BspTNI GGTCTC 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 1 cut(s) 388
BssT1I CCWWGG 1 cut(s) 149
Bst6I CTCTTC 1 cut(s) 421
BstC8I GCNNGC 1 cut(s) 156
BstDEI CTNAG 4 cut(s) 27, 58, 135, 224
BstDSI CCRYGG 1 cut(s) 149
BstHHI GCGC 2 cut(s) 238, 378
BstKTI GATC 1 cut(s) 391
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 1 cut(s) 388
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 158
BstSFI CTRYAG 1 cut(s) 364
BstSLI GKGCMC 1 cut(s) 110
BtgI CCRYGG 1 cut(s) 149
BtsIMutI CAGTG 1 cut(s) 143
BveI ACCTGC 2 cut(s) 306, 371
Cac8I GCNNGC 1 cut(s) 156
CfoI GCGC 2 cut(s) 238, 378
Csp6I GTAC 1 cut(s) 324
CviAII CATG 3 cut(s) 150, 220, 307
CviJI RGCY 3 cut(s) 11, 62, 187
CviKI_1 RGCY 3 cut(s) 11, 62, 187
CviQI GTAC 1 cut(s) 324
DdeI CTNAG 4 cut(s) 27, 58, 135, 224
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
Eam1104I CTCTTC 1 cut(s) 421
EarI CTCTTC 1 cut(s) 421
Eco130I CCWWGG 1 cut(s) 149
Eco31I GGTCTC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 209
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
FaeI CATG 3 cut(s) 153, 223, 310
FaiI YATR 6 cut(s) 14, 65, 151, 221, 279, 308
FatI CATG 3 cut(s) 149, 219, 306
FspI TGCGCA 1 cut(s) 237
GlaI GCGC 2 cut(s) 237, 377
HapII CCGG 1 cut(s) 159
HhaI GCGC 2 cut(s) 238, 378
Hin1II CATG 3 cut(s) 153, 223, 310
Hin6I GCGC 2 cut(s) 236, 376
HinP1I GCGC 2 cut(s) 236, 376
HinfI GANTC 2 cut(s) 16, 98
HpaII CCGG 1 cut(s) 159
HphI GGTGA 1 cut(s) 352
Hpy188I TCNGA 3 cut(s) 30, 103, 440
HpyAV CCTTC 2 cut(s) 196, 414
HpyCH4V TGCA 3 cut(s) 117, 315, 366
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 4 cut(s) 27, 58, 135, 224
Hsp92II CATG 3 cut(s) 153, 223, 310
HspAI GCGC 2 cut(s) 236, 376
Kzo9I GATC 1 cut(s) 388
LpnPI CCDG 5 cut(s) 172, 301, 376, 390, 433
MaeIII GTNAC 1 cut(s) 358
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MboII GAAGA 3 cut(s) 202, 253, 438
MhlI GDGCHC 2 cut(s) 110, 136
MluCI AATT 4 cut(s) 32, 112, 287, 382
MmeI TCCRAC 1 cut(s) 418
MnlI CCTC 5 cut(s) 79, 87, 205, 246, 249
MseI TTAA 3 cut(s) 48, 180, 354
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 160
MwoI GCNNNNNNNGC 1 cut(s) 114
NciI CCSGG 1 cut(s) 160
NcoI CCATGG 1 cut(s) 149
NdeII GATC 1 cut(s) 388
NlaIII CATG 3 cut(s) 153, 223, 310
NlaIV GGNNCC 2 cut(s) 147, 230
NmuCI GTSAC 1 cut(s) 358
NsbI TGCGCA 1 cut(s) 237
PaqCI CACCTGC 2 cut(s) 306, 371
PfeI GAWTC 2 cut(s) 16, 98
PspN4I GGNNCC 2 cut(s) 147, 230
PstI CTGCAG 1 cut(s) 368
RsaI GTAC 1 cut(s) 325
RsaNI GTAC 1 cut(s) 324
SaqAI TTAA 3 cut(s) 48, 180, 354
Sau3AI GATC 1 cut(s) 388
ScrFI CCNGG 1 cut(s) 160
SduI GDGCHC 2 cut(s) 110, 136
SfcI CTRYAG 1 cut(s) 364
Sse9I AATT 4 cut(s) 32, 112, 287, 382
SsiI CCGC 3 cut(s) 173, 310, 321
StyD4I CCNGG 1 cut(s) 158
StyI CCWWGG 1 cut(s) 149
TasI AATT 4 cut(s) 32, 112, 287, 382
TfiI GAWTC 2 cut(s) 16, 98
Tru1I TTAA 3 cut(s) 48, 180, 354
Tru9I TTAA 3 cut(s) 48, 180, 354
TscAI CASTG 1 cut(s) 143
TseFI GTSAC 1 cut(s) 358
Tsp45I GTSAC 1 cut(s) 358
TspGWI ACGGA 1 cut(s) 432
TspRI CASTG 1 cut(s) 143
XapI RAATTY 2 cut(s) 32, 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.