Rorug02G0101200

CRAL-TRIO domain-containing protein C23B6.04c-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007647
Physical Location & Seq
Forward (+)
8591138 .. 8592196
1059 bp
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UTR
Exon/CDS
Intron
Rorug02G0101200.1

Sequence Viewer

Length: 381 bp
ATGATGCCTTCTCCTTCACATCTATCTTGCTTGAAGGCTCGAGGAAGTCCGAGGCAGTCTCAGTCGATGACAAAAGAGACCTCAAATCAGTCATGGCTTCTCTTCTCAAATCGGCTCGCTCTCCCACAAAAGCAGGATAAGAACTCAAAGGAGGATGCATTGTTGATGAAGCTTGCAAAGGAGAAGCACTGGAGGAGGTGCCCAAGGTGTGAGTTCTTTGTGGAACGTTCATCAGGCTGCAATAAAATGAAGTGCAGGTGCGGACATCGTTTCTGCTACAGTTGTGGAGGAGCCAAATGGCCCAAATCAGCCAAATGTGGTTGTGGACTTTACGGGCGAAATGGAAATCATTGTCTGATTCTGAGAAAGCACCCTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

126

Amino Acids

14.41

Weight (kDa)

9.91

Isoelectric Point (pI)

66.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rcat_RNF216 PF26200 64 - 96 7.7e-06 RNF216 Rcat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 246
Acc36I ACCTGC 1 cut(s) 246
AccB1I GGYRCC 1 cut(s) 198
AciI CCGC 1 cut(s) 261
AclI AACGTT 1 cut(s) 226
AgsI TTSAA 1 cut(s) 34
AluBI AGCT 1 cut(s) 172
AluI AGCT 1 cut(s) 172
Alw26I GTCTC 2 cut(s) 63, 71
Ama87I CYCGRG 1 cut(s) 39
AoxI GGCC 1 cut(s) 299
ApeKI GCWGC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 300
AvaI CYCGRG 1 cut(s) 39
BaeGI GKGCMC 1 cut(s) 203
BanI GGYRCC 1 cut(s) 198
BbvI GCAGC 1 cut(s) 224
BcoDI GTCTC 2 cut(s) 63, 71
BfmI CTRYAG 1 cut(s) 277
BfuAI ACCTGC 1 cut(s) 246
BisI GCNGC 1 cut(s) 238
BlsI GCNGC 1 cut(s) 239
BmeT110I CYCGRG 1 cut(s) 39
BmgT120I GGNCC 1 cut(s) 300
BmiI GGNNCC 2 cut(s) 200, 292
BmsI GCATC 1 cut(s) 145
BplI GAGNNNNNCTC 2 cut(s) 43, 75
BpmI CTGGAG 1 cut(s) 211
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 2 cut(s) 50, 203
Bse1I ACTGG 1 cut(s) 194
BseDI CCNNGG 2 cut(s) 50, 203
BseGI GGATG 1 cut(s) 160
BseMII CTCAG 2 cut(s) 74, 353
BseNI ACTGG 1 cut(s) 194
BseRI GAGGAG 2 cut(s) 208, 303
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 1 cut(s) 224
BsgI GTGCAG 1 cut(s) 274
BshFI GGCC 1 cut(s) 301
BshNI GGYRCC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 39
BsmAI GTCTC 2 cut(s) 63, 71
BsnI GGCC 1 cut(s) 301
Bso31I GGTCTC 1 cut(s) 71
BsoBI CYCGRG 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 203
BspACI CCGC 1 cut(s) 261
BspANI GGCC 1 cut(s) 301
BspCNI CTCAG 2 cut(s) 73, 354
BspLI GGNNCC 2 cut(s) 200, 292
BspMI ACCTGC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 198
BspTNI GGTCTC 1 cut(s) 71
BsrI ACTGG 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 50, 203
BssT1I CCWWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 281
Bst6I CTCTTC 1 cut(s) 107
BstC8I GCNNGC 2 cut(s) 117, 174
BstDEI CTNAG 2 cut(s) 60, 362
BstF5I GGATG 1 cut(s) 160
BstMAI GTCTC 2 cut(s) 63, 71
BstSFI CTRYAG 1 cut(s) 277
BstSLI GKGCMC 1 cut(s) 203
BstV1I GCAGC 1 cut(s) 224
BsuRI GGCC 1 cut(s) 301
BtsCI GGATG 1 cut(s) 160
BtsIMutI CAGTG 1 cut(s) 187
BveI ACCTGC 1 cut(s) 246
Cac8I GCNNGC 2 cut(s) 117, 174
Cfr13I GGNCC 1 cut(s) 300
CviAII CATG 1 cut(s) 93
CviJI RGCY 8 cut(s) 38, 97, 115, 172, 237, 293, 301, 311
CviKI_1 RGCY 8 cut(s) 38, 97, 115, 172, 237, 293, 301, 311
DdeI CTNAG 2 cut(s) 60, 362
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
Eco130I CCWWGG 1 cut(s) 203
Eco31I GGTCTC 1 cut(s) 71
Eco88I CYCGRG 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 203
EcoT22I ATGCAT 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 1 cut(s) 96
FaiI YATR 1 cut(s) 94
FatI CATG 1 cut(s) 92
Fnu4HI GCNGC 1 cut(s) 238
FokI GGATG 1 cut(s) 167
Fsp4HI GCNGC 1 cut(s) 238
GluI GCNGC 1 cut(s) 238
GsuI CTGGAG 1 cut(s) 211
HaeIII GGCC 1 cut(s) 301
Hin1II CATG 1 cut(s) 96
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 1 cut(s) 358
Hpy166II GTNNAC 1 cut(s) 326
Hpy188I TCNGA 4 cut(s) 51, 357, 363, 380
Hpy8I GTNNAC 1 cut(s) 326
HpyAV CCTTC 3 cut(s) 18, 24, 28
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 4 cut(s) 158, 176, 240, 255
HpyF3I CTNAG 2 cut(s) 60, 362
HpySE526I ACGT 1 cut(s) 226
Hsp92II CATG 1 cut(s) 96
LmnI GCTCC 1 cut(s) 290
LpnPI CCDG 4 cut(s) 119, 175, 219, 241
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 1 cut(s) 145
MaeII ACGT 1 cut(s) 226
MboII GAAGA 1 cut(s) 94
MhlI GDGCHC 1 cut(s) 203
MnlI CCTC 7 cut(s) 35, 45, 91, 145, 186, 189, 281
Mph1103I ATGCAT 1 cut(s) 160
NlaIII CATG 1 cut(s) 96
NlaIV GGNNCC 2 cut(s) 200, 292
NsiI ATGCAT 1 cut(s) 160
PaeR7I CTCGAG 1 cut(s) 39
PaqCI CACCTGC 1 cut(s) 246
PfeI GAWTC 1 cut(s) 358
PkrI GCNGC 1 cut(s) 239
Psp1406I AACGTT 1 cut(s) 226
PspN4I GGNNCC 2 cut(s) 200, 292
PspPI GGNCC 1 cut(s) 300
PspXI VCTCGAGB 1 cut(s) 39
SatI GCNGC 1 cut(s) 238
Sau96I GGNCC 1 cut(s) 300
SduI GDGCHC 1 cut(s) 203
SetI ASST 6 cut(s) 83, 174, 200, 209, 229, 260
SfaNI GCATC 1 cut(s) 145
SfcI CTRYAG 1 cut(s) 277
Sfr274I CTCGAG 1 cut(s) 39
SlaI CTCGAG 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 39
SmoI CTYRAG 1 cut(s) 39
SsiI CCGC 1 cut(s) 261
StyI CCWWGG 1 cut(s) 203
TaaI ACNGT 1 cut(s) 281
TaiI ACGT 1 cut(s) 229
TaqI TCGA 2 cut(s) 40, 65
TfiI GAWTC 1 cut(s) 358
TscAI CASTG 1 cut(s) 194
TseI GCWGC 1 cut(s) 237
TspDTI ATGAA 3 cut(s) 182, 219, 263
TspRI CASTG 1 cut(s) 194
XhoI CTCGAG 1 cut(s) 39
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.