Rorug02G0116100

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017398
Physical Location & Seq
Forward (+)
9878005 .. 9878798
794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0116100.1

Sequence Viewer

Length: 429 bp
ATGGGAACTTCCGGTAACTGGTTCAGAAGAATCCGAAAAAAGTTTGTGAGATCCTCCGACAGAGATATCATTGTCATCTATTCCAACACCAGCCCAAGCTCCCATGGAGAAGGAGCTGATTATGAAGTCCTCACTCGCGACGATCCTTCATCTACAATGTCTTCGTTCAGAACCAGAGAGAGAATTTTAACCAAGGAAGACATTGCAGCCATCAAGATCCAAGCATTTTTCAGAGGCCAACTTGCAAGACAAGCATTTCGAGCACTCAAGAGCCTGGTGAAGCTGCAAGCACTGGTTAGAGGAGTGTGCGTGAGGAAACAGGCGAGAGTCGCTATGCAATGCATGCGTGCACTTGTCCAGTTGCAGGTCAAGGTTCGCGCACGGCAGCTCCTCAACGGGTATACTGATGCCAGATCCATCACAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

142

Amino Acids

16.14

Weight (kDa)

11.21

Isoelectric Point (pI)

32.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 355
AccI GTMKAC 1 cut(s) 401
AccII CGCG 2 cut(s) 138, 378
AclWI GGATC 4 cut(s) 45, 137, 211, 408
AcsI RAATTY 1 cut(s) 183
AfiI CCNNNNNNNGG 2 cut(s) 18, 364
AjnI CCWGG 1 cut(s) 273
AluBI AGCT 5 cut(s) 99, 116, 283, 388, 425
AluI AGCT 5 cut(s) 99, 116, 283, 388, 425
Alw21I GWGCWC 2 cut(s) 265, 352
Alw44I GTGCAC 1 cut(s) 348
AlwI GGATC 4 cut(s) 45, 137, 211, 408
AoxI GGCC 1 cut(s) 235
ApaLI GTGCAC 1 cut(s) 348
ApeKI GCWGC 3 cut(s) 206, 283, 385
ApoI RAATTY 1 cut(s) 183
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
AspLEI GCGC 1 cut(s) 380
AsuHPI GGTGA 1 cut(s) 289
BaeGI GKGCMC 1 cut(s) 352
BbsI GAAGAC 2 cut(s) 153, 204
Bbv12I GWGCWC 2 cut(s) 265, 352
BbvI GCAGC 3 cut(s) 218, 270, 397
BccI CCATC 2 cut(s) 218, 425
BceAI ACGGC 1 cut(s) 398
BciT130I CCWGG 1 cut(s) 275
BfuAI ACCTGC 1 cut(s) 355
BisI GCNGC 3 cut(s) 207, 284, 386
BlsI GCNGC 3 cut(s) 208, 285, 387
Bme1390I CCNGG 1 cut(s) 275
BmrFI CCNGG 1 cut(s) 275
BmsI GCATC 1 cut(s) 397
BpiI GAAGAC 2 cut(s) 153, 204
BpuEI CTTGAG 1 cut(s) 251
BsaJI CCNNGG 2 cut(s) 103, 192
BsaWI WCCGGW 1 cut(s) 11
BsaXI ACNNNNNCTCC 4 cut(s) 294, 324, 372, 402
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 364
Bse1I ACTGG 3 cut(s) 23, 297, 358
Bse3DI GCAATG 2 cut(s) 201, 344
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 2 cut(s) 103, 192
BseLI CCNNNNNNNGG 2 cut(s) 18, 364
BseMI GCAATG 2 cut(s) 201, 344
BseNI ACTGG 3 cut(s) 23, 297, 358
BseRI GAGGAG 2 cut(s) 315, 380
BseSI GKGCMC 1 cut(s) 352
BseXI GCAGC 3 cut(s) 218, 270, 397
Bsh1236I CGCG 2 cut(s) 138, 378
BshFI GGCC 1 cut(s) 237
BsiHKAI GWGCWC 2 cut(s) 265, 352
BsiSI CCGG 1 cut(s) 12
BslI CCNNNNNNNGG 2 cut(s) 18, 364
BsnI GGCC 1 cut(s) 237
Bsp1286I GDGCHC 2 cut(s) 265, 352
Bsp143I GATC 4 cut(s) 50, 142, 216, 413
Bsp19I CCATGG 1 cut(s) 103
Bsp68I TCGCGA 1 cut(s) 138
BspANI GGCC 1 cut(s) 237
BspFNI CGCG 2 cut(s) 138, 378
BspMI ACCTGC 1 cut(s) 355
BspPI GGATC 4 cut(s) 45, 137, 211, 408
BsrDI GCAATG 2 cut(s) 201, 344
BsrI ACTGG 3 cut(s) 23, 297, 358
BssECI CCNNGG 2 cut(s) 103, 192
BssMI GATC 4 cut(s) 50, 142, 216, 413
BssNAI GTATAC 1 cut(s) 402
BssT1I CCWWGG 2 cut(s) 103, 192
Bst1107I GTATAC 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 275
BstAPI GCANNNNNTGC 1 cut(s) 343
BstC8I GCNNGC 3 cut(s) 288, 344, 348
BstDSI CCRYGG 1 cut(s) 103
BstFNI CGCG 2 cut(s) 138, 378
BstHHI GCGC 1 cut(s) 380
BstKTI GATC 4 cut(s) 53, 145, 219, 416
BstMBI GATC 4 cut(s) 50, 142, 216, 413
BstMWI GCNNNNNNNGC 4 cut(s) 251, 260, 329, 343
BstNI CCWGG 1 cut(s) 275
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 1 cut(s) 273
BstSLI GKGCMC 1 cut(s) 352
BstUI CGCG 2 cut(s) 138, 378
BstV1I GCAGC 3 cut(s) 218, 270, 397
BstV2I GAAGAC 2 cut(s) 153, 204
BstX2I RGATCY 3 cut(s) 50, 216, 413
BstYI RGATCY 3 cut(s) 50, 216, 413
BstZ17I GTATAC 1 cut(s) 402
BsuRI GGCC 1 cut(s) 237
BtgI CCRYGG 1 cut(s) 103
BtsIMutI CAGTG 1 cut(s) 290
BtuMI TCGCGA 1 cut(s) 138
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 3 cut(s) 288, 344, 348
CfoI GCGC 1 cut(s) 380
CviAII CATG 2 cut(s) 104, 343
CviJI RGCY 9 cut(s) 93, 99, 116, 209, 237, 273, 283, 388, 425
CviKI_1 RGCY 9 cut(s) 93, 99, 116, 209, 237, 273, 283, 388, 425
DpnI GATC 4 cut(s) 52, 144, 218, 415
DpnII GATC 4 cut(s) 50, 142, 216, 413
Eco130I CCWWGG 2 cut(s) 103, 192
Eco32I GATATC 1 cut(s) 67
EcoRII CCWGG 1 cut(s) 273
EcoRV GATATC 1 cut(s) 67
EcoT14I CCWWGG 2 cut(s) 103, 192
EcoT22I ATGCAT 1 cut(s) 344
ErhI CCWWGG 2 cut(s) 103, 192
FaeI CATG 2 cut(s) 107, 346
FaiI YATR 5 cut(s) 105, 123, 335, 344, 402
FatI CATG 2 cut(s) 103, 342
FblI GTMKAC 1 cut(s) 401
Fnu4HI GCNGC 3 cut(s) 207, 284, 386
Fsp4HI GCNGC 3 cut(s) 207, 284, 386
GlaI GCGC 1 cut(s) 379
GluI GCNGC 3 cut(s) 207, 284, 386
HaeIII GGCC 1 cut(s) 237
HapII CCGG 1 cut(s) 12
HhaI GCGC 1 cut(s) 380
Hin1II CATG 2 cut(s) 107, 346
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HinfI GANTC 2 cut(s) 30, 327
HpaII CCGG 1 cut(s) 12
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 2 cut(s) 350, 402
Hpy188I TCNGA 5 cut(s) 26, 35, 58, 170, 233
Hpy188III TCNNGA 3 cut(s) 137, 214, 268
Hpy8I GTNNAC 2 cut(s) 350, 402
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 2 cut(s) 104, 156
HpyCH4V TGCA 7 cut(s) 206, 245, 286, 337, 342, 350, 364
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 260, 329, 343
Hsp92II CATG 2 cut(s) 107, 346
HspAI GCGC 1 cut(s) 378
Kzo9I GATC 4 cut(s) 50, 142, 216, 413
LmnI GCTCC 3 cut(s) 104, 113, 393
Lsp1109I GCAGC 3 cut(s) 218, 270, 397
LweI GCATC 1 cut(s) 397
MaeIII GTNAC 1 cut(s) 14
MalI GATC 4 cut(s) 52, 144, 218, 415
MboI GATC 4 cut(s) 50, 142, 216, 413
MboII GAAGA 3 cut(s) 39, 153, 209
MflI RGATCY 3 cut(s) 50, 216, 413
MhlI GDGCHC 2 cut(s) 265, 352
MluCI AATT 1 cut(s) 183
MlyI GAGTC 1 cut(s) 336
MmeI TCCRAC 2 cut(s) 81, 108
MnlI CCTC 6 cut(s) 64, 140, 227, 293, 306, 401
Mph1103I ATGCAT 1 cut(s) 344
MseI TTAA 2 cut(s) 188, 427
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 1 cut(s) 275
MvaI CCWGG 1 cut(s) 275
MvnI CGCG 2 cut(s) 138, 378
MwoI GCNNNNNNNGC 4 cut(s) 251, 260, 329, 343
NcoI CCATGG 1 cut(s) 103
NdeII GATC 4 cut(s) 50, 142, 216, 413
NlaIII CATG 2 cut(s) 107, 346
NruI TCGCGA 1 cut(s) 138
NsiI ATGCAT 1 cut(s) 344
NspI RCATGY 1 cut(s) 346
PaeI GCATGC 1 cut(s) 346
PfeI GAWTC 1 cut(s) 30
PkrI GCNGC 3 cut(s) 208, 285, 387
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 273
PspGI CCWGG 1 cut(s) 273
PsuI RGATCY 3 cut(s) 50, 216, 413
RruI TCGCGA 1 cut(s) 138
SaqAI TTAA 2 cut(s) 188, 427
SatI GCNGC 3 cut(s) 207, 284, 386
Sau3AI GATC 4 cut(s) 50, 142, 216, 413
SchI GAGTC 1 cut(s) 336
ScrFI CCNGG 1 cut(s) 275
SduI GDGCHC 2 cut(s) 265, 352
SetI ASST 7 cut(s) 101, 118, 285, 369, 375, 390, 427
SfaNI GCATC 1 cut(s) 397
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
SphI GCATGC 1 cut(s) 346
Sse9I AATT 1 cut(s) 183
StyD4I CCNGG 1 cut(s) 273
StyI CCWWGG 2 cut(s) 103, 192
TaqI TCGA 1 cut(s) 259
TasI AATT 1 cut(s) 183
TfiI GAWTC 1 cut(s) 30
Tru1I TTAA 2 cut(s) 188, 427
Tru9I TTAA 2 cut(s) 188, 427
TscAI CASTG 1 cut(s) 297
TseI GCWGC 3 cut(s) 206, 283, 385
TspDTI ATGAA 2 cut(s) 138, 138
TspRI CASTG 1 cut(s) 297
VneI GTGCAC 1 cut(s) 348
XapI RAATTY 1 cut(s) 183
XceI RCATGY 1 cut(s) 346
XmiI GTMKAC 1 cut(s) 401
Zsp2I ATGCAT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.