Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0013962

Physical Location & Seq

Genomic Coordinates Reverse (-)

9987419 .. 9994988

Gene Structure 7570 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0117100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5208 bp

ATGGATTTCCGGTTCCCGTACTCGCCTCTGGAGGTTGCGACGCCGAGCTCCGTCCAGTTCGGCATCCTCAGCCCCGGCGAGATCGGGCAAATGTCGGTGCTGGAGGTCGAGGATGGCCGTTCAATGCTGAAAGGGAAGCCGGTGAGAGGCGGCTTGAGTGATCCTCGGCTTGGTCCGATTGACAGGCACAACAGGTGTGAGACTTGTGCCAATAACTTGAGCGAGTGCCCGGGCCATTTCGGCCACATTGAGCTCGCCAAGCCTGTGTTTCACTTCGGGTTTATGAAGACTGTGCTGTCTGTAATGCGCTGCGTTTGCTTCAATTGCTCCAAACTTCTACTCGATGAGGAAGATGTTAAGTTTAAACAAGCTTTGAGAATCAAGAAGCCTAAAGATAGGCTAAAAAAGTTTGTGGATGCATGCAAAAACAAGACCAGGTGTGAAGGTGGTGATGAAATTGATCTGCAAGGCTCAAAATCCGGTCAACATGTAAAGCAAAGTCGGGGTGGCTGTGGTGCTCAGCAGCCCAAGTTCTCTATGGCTGGTTTGGAACTGGTTGCAGAGTACAAATCACAAAGGAAACAATATGATGAGGAACAGCTTCCTGAACCAGTGGAAAGGAAACAAATCCTTACTGCAGAAAAGGTTCTTAGCTTATTAAAGAGGATAAGTGATGAGGACTGCCAATTGTTGGGACTGAACCCTGAATATGCTCGACCAGATTGGATGATTTTACAAGTTCTTCCTATTCCTCCACCAGCTGTGAGACCCCCGGTGATGATGAGCAGCTCTGCCATGAGTGAGGATGATTTAACACATCAGTTGTCCATGATTATTCGCCACAACGAGAATTTGAGGAAACAGGAAAAACATGGTTCAGCTGCCCATATCATTTCACAGTTTACACAAATCTTGCAATACTACATATCCACATATTTTGACAATAGCTTGCCTGGACTTCCAAAGGCTGCTCATGGATCGACAAAGAGGCCCATTAAATCAATATGTTGTAGGCTTAAGGCAAAGCATGGCCGGATTAGAGGAAACTTGATGGGGAAGAGAGTCGATTTCTCAGCACGGACAGTTATTACACCAGATCCGAACCTTAGCCTTGATCAATTAGGAGTACCATGGAGCATTGCTATGAATCTCACATACACAGAGACTGTGACTCCATTTAATAAAGAAGGGTTGAAGGAGCTTGTTGAGTATGGGCCACATCCCCCACCTGGACTAACTGGTGCAAAGTACATCATACGAGATGATGGCCAAAGACTCGATCTTCGTTACGTGGAGCGTCATCTAAAGGATGGAGACATTGTCCTCTTAAATCGTCAGCCTAGTCTCCATAAAATGTCATTCATGGGGCATAGAATCAAGATCATGCCATACTCAACTCTTCGCTTGAACTTATCTGTCACTTCACCTTACAATGCTGATTTTGATGGGGATGAAATGAACGTCCATGTTCCTCAGTCATTTGAAACTAGGGCAGAAGCTCTGGAGCTCATGATGGTGCCCAAATGCATTGTTTCCCCTCAGTCAAACCGGCCTGTAATGGGAATCGTCCAAGATACACTGTTAGGTGCTCGAAAAATCACAAAAAGGGATACCTTCATAGAGAAGGACGTTTTTATGAACATCTTGATGTGGTGGGAGGATTTTGACGGGAAAGTGCCTGCTCCTGCAATATTGAAGCCACGACCTCTTTGGACTGGGAAACAGGTGTTTAATCTTATAATACCGAAACAGATAAATCTCCAAAGAACTTCAGCGTGGCACTCTGAATCAGAAAGGGGTAACATTACCCCTGGTGATACTTTCGTTAGAATAGAAAAAGGGGAGTTACTTTCTGGAACTCTCTGCAAAAGGGCACTTGGGACTTCTACAGGAAGTCTTATACATGTCATATGGGAAGAGGTTGGTCCAGATGCAGCCTCCAAATTCCTTAGTAATACCCAGTGGCTTGTGAATTACTGGCTGTTGCAGGATGGTTTTAGCATTGGAATTAGAGATACAATTGCCGATGCAGCAACAATGGAAAAGATCAATGTAACTATTTACAAGGCAAAAAATGAAGTGAAAGAACTTATCAGTAAAGCCCAATCCAGGCAGCTGGAGGCTGAACCAGGACGAACTATGATGGAATCCTTTGAAAACAAAGTGAACCAGGTGTTGAATAGGGCTCGTGATGATGCTGGAAGTAGTGCCCAGAAGAGTTTATCTGAGAGTAACAATCTCAAAGCCATGGTCACAGCCGGATCGAAGGGAAGTTTTATCAATATATCGCAGATGACTGCTTGTGTGGGACAGCAGAATGTTGAGGGTAAGCGAATTCCGTATGGATTCACAGACCGGACTTTGCCCCATTTTACGAAAGACGATTATGGACCGGAAAGTCGTGGCTTTGTAGAGAATTCCTACCTGCGTGGATTAACTCCACATGAGTTTTATTTTCATGCTATGGGTGGTAGGGAAGGCATTATTGATACTGCAATTAAGACATCTGAGACTGGGTATATCCAGAGGAGACTGGTGAAGGCTATGGAGGATATTATGGTTAAATATGATGGGACTGTCAGAAATTCATTGGGGGATGTAATACAGTTTCTCTATGGGGAAGATGGAATGGACGCTGTTTGGTTAGAATCACAAAAGCTGGATTCTTTAAAGATGAAAAAATCAGAATTTGACAAGACTTTCACTTTTGAGTTTGATGATAGAAATTGGAATCCGGACTATATGTTGCCTGAATATGTTGAGGATCTAAAAACTATTGGAGAGTTTCGCTGGGCATTGGACGCTGAAGTTTCAAAACTTGAAGCTGATAGGATTCAACTTGGCACAGAGATTGCTGTCACAGGTGATAATACTTGGCCGTTGCCTGTTAACCTGAAGAGGCTCATTTGGAATGCACAAAAAACTTTCAAAATTGACTTCCGAAGGACTTCTGATATGCAACCAATGGAAATTATAGAAGCTATTGATAATCTTCAAGAGAGGCTGAAGGTTGTTCCTGGTGATGACTTGTTAAGTGTTAAAGCTCAAAATAATGCTACACTCTTTTTCAACATTTTGCTTCGCAGCACTTTTGCTAGCAAAAAGGTGCTAAAAGAGTACAAGCTTACTCGTGAAGCATTTGAGTGGGTTATTGGGGAGATAGAGGCACGCTTCCTGCAATCACTTGTAGCACCTGGGGAAATGATCGGTATTGTTGCTGCACAATCTATTGGCGAGACTGCCACTCAGATGACTCTCAATACCTTCCACTATGCCGGTGTAAGTGCAAAGAATGTTACTCTAGGTGTTCCAAGGTTGAAGGAAATTATTGATGTCTCTAAGCGTATCAAAACACCATCTCTCTCTATCTACTTGAAGCCTGAAGCTAATAAAACCAAGGAGAAGGCCAAGAATGTTCAGTGTGCTTTGGAATACACTAACCTCGGAAGTGTTACTCAAGCTACAGAATTGTGGTATGATCCGGACCCTATGAGCACAATAATTGAAGAGGATCTTGAATTTGTCCAATCTTACTATGAAATGCCAGATGAAGAGGTTAACCCTGAAAAAATATCACCTTGGTTGCTTCGCATAGAGATGAATCGTGAAATGATGGTGGATAAGAAACTGAGTATGGCTGACATTGCTGAGAAGATTAATTGTGAATATAGTGGTAAAATACAGTGCATCTTTAATGATGACAATTCTGAGAAGTTGATACTACGAATCCGTCTCATGAATGAAGAAGCTCCACATGAGGAACTGAATGATCAATCTGAAGATGATGTTTTCCTGAAGATGATTGAGAGTAACATGCTTACAAAAATGGCTCTTCAAGGGATCCCAGAGATTAAAAAAGTGTTCATAAAGCATGGTAAAGTTAACAAGTTTGATCAGAATGAAGGGTTTAATGCGGAGCAAGAGTGGATGCTGGATACTGAAGGTGTTAATCTCTTAGCTGTCATGTGCCATGAGGATGTTGATGCCAAGAGGACAACAAGCAATGACTTGTATGAAATTATGGAAGTTCTTGGGATTGAGGCAGCTCGACGGGCATTGCTGGATGAGTTGCGTAATGTTATATCATTTGATGGATCCTATGTAAATTACCGGCACCTGGCCATTCTTTGTGATACCATGACCTATCGTGGCTACTTGATGGCCATCACTCGACATGGTATCAATCGAAAGGACACTGGTCCTATGATGCGATGCTCGTTTGAACAAACAGTGGACATGCTGCTGGATGCTTCAGTTTTTGCAGAAACAGATCATCTGAGGGGTGCAACAGAAAACATAATGCTAGGCAAGCTGGCACCAATTGGCACTGGAGACTGTGCCCTGTATCTGAATGAAGAGATGTTACAAAATGCTATGGAAGTGCAGCTACCTGGTTATATGGATGGCCTTGATGTCGGCATGACACCATCCCATTCTCCATACCTTGAGAGCATACTGTCTCCTAGTTATTTGCCGAGTCCCAGTAATCCTCTTTCACCGATGTTGGATGCAGCACAGTTTTCTCCATACGTTAGGGGAATTCCCTTTTCTCCTACTTCATCTCCAGGCAACATCCCATCATCTCTTGGCTACTATCCGCCATCCTTTGTATACAGTCCCACCTCCCCATACTATAGCCCCACGTCACCAGGTTATAGTCCAACCAGTCCCCCCTATTCTCCTTCACGTCCATCTTTCACATCACCAGGTTATACTCCAACCAGTCCTCCCTATTCTCCTTCACGTCCATCTTTCACAGCACCAGGTTATAGTCCAACCAGTCCTTCCCACTTCTCCTTTGTACACCCCAACATCACCCTCCTACAGCCCGACCTCTCCAAGCTACAGCCCGACTTTCCCACATACACCCCCACTTCGCTTTCCTACAGCCCCACATCGCCATCATACAGCCCCACATCCCCATCATACAGCCCCACATCACCCTCCTACAGCCCTAGCTCCCCATACGATAGCCTGACATCTCCAGGTTACAACCCAACCTCTCCCAGTTATAGCCTGACATCTCTGTACAGCCCAACTTCACTAGCATATTCCCCAACATCTCCAACTTACTCTCCATTGAGCCCCACTTACAGTCCCAGCAGCCTATTCACCACCTCTGCAGTGAGCCCAGACTATTCCCCAAGTTCTCCACAGTACAGTCCAAGTGGTGAGTACTCATTGCGACATAGCGAAAAGGATGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000976 GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009751 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042221 GO:0042493 GO:0042545 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045229 GO:0045787 GO:0045893 GO:0045935 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051726 GO:0060255 GO:0065007 GO:0071554 GO:0071555 GO:0071840 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-Dof

Protein Analysis

1735

Amino Acids

194.7

Weight (kDa)

5.77

Isoelectric Point (pI)

53.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	16 - 350	1.7e-100	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	352 - 506	1.2e-60	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	509 - 673	3.3e-53	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	700 - 804	1.1e-38	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	811 - 1400	1.2e-103	RNA polymerase Rpb1, domain 5
RNA_pol_Rpb1_6	PF04992	877 - 1061	1.5e-64	RNA polymerase Rpb1, domain 6
RNA_pol_Rpb1_7	PF04990	1146 - 1279	3.4e-50	RNA polymerase Rpb1, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013962)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G50410 AT5G66940
fragaria_vesca	FvH4_1g14790
malus_domestica	MD02G1161200.v1.1 MD15G1275300.v1.1
prunus_persica	Prupe.7G142500_v2.0.a1
pyrus_communis	pycom02g12720 pycom15g23830
rosa_chinensis	RchiOBHm_Chr2g0103751
rosa_laevigata	RLG00000017255
rosa_multiflora	Rmu_sc0001852.1_g000001
rosa_rugosa	Rorug02G0117100
rosa_samantha	Rh2AG166000 Rh2BG173300 Rh2CG172000 Rh2DG171500
rosa_wichuraiana	Rw2G013000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1739
AasI	GACNNNNNNGTC	2 cut(s)	295, 2397
Acc36I	ACCTGC	1 cut(s)	2433
AccB1I	GGYRCC	3 cut(s)	1516, 4113, 4315
AccB7I	CCANNNNNTGG	1 cut(s)	691
AccI	GTMKAC	1 cut(s)	4611
AccIII	TCCGGA	2 cut(s)	2734, 3481
AciI	CCGC	3 cut(s)	150, 3914, 4598
AcoI	YGGCCR	7 cut(s)	115, 241, 1030, 1267, 2876, 4119, 4161
AcsI	RAATTY	8 cut(s)	850, 1943, 2334, 2416, 2584, 2687, 3518, 4539
AcuI	CTGAAG	9 cut(s)	1755, 2826, 2915, 3026, 3402, 3798, 3815, 3960, 4236
AcyI	GRCGYC	1 cut(s)	41
AfaI	GTAC	9 cut(s)	20, 566, 1128, 1250, 3119, 4803, 5030, 5159, 5177
AfiI	CCNNNNNNNGG	7 cut(s)	146, 170, 691, 1229, 1559, 2109, 4117
AflII	CTTAAG	1 cut(s)	1016
AflIII	ACRYGT	2 cut(s)	487, 1903
AjiI	CACGTC	3 cut(s)	4644, 4688, 4745
AjuI	GAANNNNNNNTTGG	2 cut(s)	4768, 4800
AloI	GAACNNNNNNTCC	1 cut(s)	28
Alw21I	GWGCWC	6 cut(s)	50, 255, 520, 1509, 1591, 3497
AlwNI	CAGNNNCTG	1 cut(s)	1166
Ama87I	CYCGRG	1 cut(s)	229
Aor13HI	TCCGGA	2 cut(s)	2734, 3481
ApoI	RAATTY	8 cut(s)	850, 1943, 2334, 2416, 2584, 2687, 3518, 4539
ArsI	GACNNNNNNTTYG	4 cut(s)	1266, 1298, 2235, 2267
AseI	ATTAAT	1 cut(s)	3657
Asp700I	GAANNNNTTC	3 cut(s)	600, 645, 2947
AspLEI	GCGC	1 cut(s)	309
AspS9I	GGNCC	8 cut(s)	173, 232, 990, 1214, 1925, 2390, 3484, 4199
AsuC2I	CCSGG	4 cut(s)	75, 230, 231, 773
AsuNHI	GCTAGC	1 cut(s)	3095
AvaI	CYCGRG	1 cut(s)	229
AvaII	GGWCC	5 cut(s)	173, 1925, 2390, 3484, 4199
BaeGI	GKGCMC	5 cut(s)	230, 1521, 1876, 2212, 4342
BaeI	ACNNNNGTAYC	2 cut(s)	1809, 1842
BalI	TGGCCA	3 cut(s)	1269, 4121, 4163
BamHI	GGATCC	2 cut(s)	3840, 4094
BanI	GGYRCC	3 cut(s)	1516, 4113, 4315
BanII	GRGCYC	6 cut(s)	50, 255, 1509, 2188, 5087, 5132
BarI	GAAGNNNNNNTAC	4 cut(s)	4347, 4371, 4379, 4403
BauI	CACGAG	2 cut(s)	2187, 3129
BbsI	GAAGAC	1 cut(s)	293
Bbv12I	GWGCWC	6 cut(s)	50, 255, 520, 1509, 1591, 3497
BbvCI	CCTCAGC	1 cut(s)	68
BceAI	ACGGC	2 cut(s)	102, 2863
BciVI	GTATCC	2 cut(s)	1603, 3928
BclI	TGATCA	3 cut(s)	1114, 3769, 3892
BcnI	CCSGG	4 cut(s)	75, 230, 231, 773
BfaI	CTAG	8 cut(s)	1341, 1488, 3096, 3302, 4304, 4464, 4956, 5045
BfmI	CTRYAG	9 cut(s)	636, 1887, 3462, 4633, 4823, 4844, 4885, 4948, 5121
BfrI	CTTAAG	1 cut(s)	1016
BfuAI	ACCTGC	1 cut(s)	2433
BfuI	GTATCC	2 cut(s)	1603, 3928
BglI	GCCNNNNNGGC	1 cut(s)	240
BlpI	GCTNAGC	1 cut(s)	519
BmcAI	AGTACT	1 cut(s)	5177
Bme18I	GGWCC	5 cut(s)	173, 1925, 2390, 3484, 4199
BmeT110I	CYCGRG	1 cut(s)	229
BmgBI	CACGTC	3 cut(s)	4644, 4688, 4745
BmgT120I	GGNCC	8 cut(s)	173, 232, 990, 1214, 1925, 2390, 3484, 4199
BmiI	GGNNCC	7 cut(s)	14, 1518, 3486, 3842, 4096, 4115, 4317
BmrI	ACTGGG	5 cut(s)	1725, 1954, 2523, 4476, 5001
BmtI	GCTAGC	1 cut(s)	3099
BmuI	ACTGGG	5 cut(s)	1725, 1954, 2523, 4476, 5001
BoxI	GACNNNNGTC	1 cut(s)	4197
BpiI	GAAGAC	1 cut(s)	293
BplI	GAGNNNNNCTC	2 cut(s)	148, 180
BpmI	CTGGAG	7 cut(s)	50, 122, 1523, 2138, 4350, 4548, 4968
Bpu10I	CCTNAGC	2 cut(s)	68, 1106
Bpu1102I	GCTNAGC	1 cut(s)	519
BpuEI	CTTGAG	4 cut(s)	175, 238, 3441, 4466
BpuMI	CCSGG	4 cut(s)	75, 230, 231, 773
BsaAI	YACGTR	1 cut(s)	1291
BsaBI	GATNNNNATC	1 cut(s)	4163
BsaHI	GRCGYC	1 cut(s)	41
BsaI	GGTCTC	1 cut(s)	760
BsaWI	WCCGGW	6 cut(s)	9, 479, 2355, 2392, 2734, 3481
Bsc4I	CCNNNNNNNGG	7 cut(s)	146, 170, 691, 1229, 1559, 2109, 4117
Bse118I	RCCGGY	4 cut(s)	139, 1548, 3275, 4110
Bse3DI	GCAATG	5 cut(s)	1137, 3642, 4009, 4055, 5180
Bse8I	GATNNNNATC	1 cut(s)	4163
BseAI	TCCGGA	2 cut(s)	2734, 3481
BseJI	GATNNNNATC	1 cut(s)	4163
BseLI	CCNNNNNNNGG	7 cut(s)	146, 170, 691, 1229, 1559, 2109, 4117
BseMI	GCAATG	5 cut(s)	1137, 3642, 4009, 4055, 5180
BseRI	GAGGAG	1 cut(s)	2542
BseSI	GKGCMC	5 cut(s)	230, 1521, 1876, 2212, 4342
BseYI	CCCAGC	2 cut(s)	2790, 5099
BsgI	GTGCAG	2 cut(s)	3204, 4403
BshNI	GGYRCC	3 cut(s)	1516, 4113, 4315
BsiHKAI	GWGCWC	6 cut(s)	50, 255, 520, 1509, 1591, 3497
BsiHKCI	CYCGRG	1 cut(s)	229
BslFI	GGGAC	8 cut(s)	708, 1894, 2322, 2587, 4464, 4602, 4653, 5082
BslI	CCNNNNNNNGG	7 cut(s)	146, 170, 691, 1229, 1559, 2109, 4117
BsmBI	CGTCTC	1 cut(s)	3737
BsmFI	GGGAC	8 cut(s)	708, 1894, 2322, 2587, 4464, 4602, 4653, 5082
BsmI	GAATGC	1 cut(s)	2917
Bso31I	GGTCTC	1 cut(s)	760
BsoBI	CYCGRG	1 cut(s)	229
Bsp13I	TCCGGA	2 cut(s)	2734, 3481
Bsp1407I	TGTACA	2 cut(s)	4801, 5028
Bsp1720I	GCTNAGC	1 cut(s)	519
Bsp19I	CCATGG	2 cut(s)	1130, 2247
BspACI	CCGC	3 cut(s)	150, 3914, 4598
BspEI	TCCGGA	2 cut(s)	2734, 3481
BspHI	TCATGA	2 cut(s)	1509, 3735
BspLI	GGNNCC	7 cut(s)	14, 1518, 3486, 3842, 4096, 4115, 4317
BspMAI	CTGCAG	2 cut(s)	640, 5125
BspMI	ACCTGC	1 cut(s)	2433
BspOI	GCTAGC	1 cut(s)	3099
BspQI	GCTCTTC	1 cut(s)	3837
BspT107I	GGYRCC	3 cut(s)	1516, 4113, 4315
BspTI	CTTAAG	1 cut(s)	1016
BspTNI	GGTCTC	1 cut(s)	760
BsrDI	GCAATG	5 cut(s)	1137, 3642, 4009, 4055, 5180
BsrFI	RCCGGY	4 cut(s)	139, 1548, 3275, 4110
BsrGI	TGTACA	2 cut(s)	4801, 5028
BssAI	RCCGGY	4 cut(s)	139, 1548, 3275, 4110
BssNAI	GTATAC	1 cut(s)	4612
BssNI	GRCGYC	1 cut(s)	41
BssSI	CACGAG	2 cut(s)	2187, 3129
BssT1I	CCWWGG	5 cut(s)	1130, 2247, 3311, 3396, 3578
Bst1107I	GTATAC	1 cut(s)	4612
Bst2BI	CACGAG	2 cut(s)	2187, 3129
Bst6I	CTCTTC	9 cut(s)	1052, 1404, 1911, 2210, 2891, 3501, 3546, 3837, 4350
BstACI	GRCGYC	1 cut(s)	41
BstAFI	CTTAAG	1 cut(s)	1016
BstAUI	TGTACA	2 cut(s)	4801, 5028
BstBAI	YACGTR	1 cut(s)	1291
BstC8I	GCNNGC	8 cut(s)	255, 421, 950, 1680, 3097, 3169, 4310, 4314
BstDSI	CCRYGG	2 cut(s)	1130, 2247
BstHHI	GCGC	1 cut(s)	309
BstNSI	RCATGY	5 cut(s)	423, 491, 1907, 3817, 4240
BstPAI	GACNNNNGTC	1 cut(s)	4197
BstSFI	CTRYAG	9 cut(s)	636, 1887, 3462, 4633, 4823, 4844, 4885, 4948, 5121
BstSLI	GKGCMC	5 cut(s)	230, 1521, 1876, 2212, 4342
BstV2I	GAAGAC	1 cut(s)	293
BstX2I	RGATCY	5 cut(s)	1096, 2764, 3511, 3840, 4094
BstXI	CCANNNNNNTGG	1 cut(s)	2116
BstYI	RGATCY	5 cut(s)	1096, 2764, 3511, 3840, 4094
BstZ17I	GTATAC	1 cut(s)	4612
BsuI	GTATCC	2 cut(s)	1603, 3928
BtgI	CCRYGG	2 cut(s)	1130, 2247
BtgZI	GCGATG	2 cut(s)	4225, 4881
BtrI	CACGTC	3 cut(s)	4644, 4688, 4745
BtsI	GCAGTG	1 cut(s)	5130
BtsIMutI	CAGTG	9 cut(s)	618, 1577, 1967, 3425, 3689, 4194, 4236, 4326, 5130
BveI	ACCTGC	1 cut(s)	2433
Cac8I	GCNNGC	8 cut(s)	255, 421, 950, 1680, 3097, 3169, 4310, 4314
CaiI	CAGNNNCTG	1 cut(s)	1166
CciI	TCATGA	2 cut(s)	1509, 3735
CfoI	GCGC	1 cut(s)	309
Cfr10I	RCCGGY	4 cut(s)	139, 1548, 3275, 4110
Cfr13I	GGNCC	8 cut(s)	173, 232, 990, 1214, 1925, 2390, 3484, 4199
Cfr9I	CCCGGG	1 cut(s)	229
CseI	GACGC	4 cut(s)	49, 1286, 2642, 2810
CsiI	ACCWGGT	6 cut(s)	434, 2169, 4390, 4648, 4705, 4762
Csp6I	GTAC	9 cut(s)	19, 565, 1127, 1249, 3118, 4802, 5029, 5158, 5176
CspCI	CAANNNNNGTGG	2 cut(s)	919, 954
CviQI	GTAC	9 cut(s)	19, 565, 1127, 1249, 3118, 4802, 5029, 5158, 5176
DraI	TTTAAA	2 cut(s)	364, 2670
DrdI	GACNNNNNNGTC	2 cut(s)	295, 2397
DseDI	GACNNNNNNGTC	2 cut(s)	295, 2397
EaeI	YGGCCR	7 cut(s)	115, 241, 1030, 1267, 2876, 4119, 4161
Eam1104I	CTCTTC	9 cut(s)	1052, 1404, 1911, 2210, 2891, 3501, 3546, 3837, 4350
EarI	CTCTTC	9 cut(s)	1052, 1404, 1911, 2210, 2891, 3501, 3546, 3837, 4350
EciI	GGCGGA	1 cut(s)	4587
Ecl136II	GAGCTC	3 cut(s)	48, 253, 1507
Eco130I	CCWWGG	5 cut(s)	1130, 2247, 3311, 3396, 3578
Eco24I	GRGCYC	6 cut(s)	50, 255, 1509, 2188, 5087, 5132
Eco31I	GGTCTC	1 cut(s)	760
Eco47I	GGWCC	5 cut(s)	173, 1925, 2390, 3484, 4199
Eco53kI	GAGCTC	3 cut(s)	48, 253, 1507
Eco57I	CTGAAG	9 cut(s)	1755, 2826, 2915, 3026, 3402, 3798, 3815, 3960, 4236
Eco88I	CYCGRG	1 cut(s)	229
EcoICRI	GAGCTC	3 cut(s)	48, 253, 1507
EcoRI	GAATTC	3 cut(s)	2334, 2416, 4539
EcoT14I	CCWWGG	5 cut(s)	1130, 2247, 3311, 3396, 3578
EcoT22I	ATGCAT	2 cut(s)	421, 1529
EcoT38I	GRGCYC	6 cut(s)	50, 255, 1509, 2188, 5087, 5132
ErhI	CCWWGG	5 cut(s)	1130, 2247, 3311, 3396, 3578
Esp3I	CGTCTC	1 cut(s)	3737
FalI	AAGNNNNNCTT	2 cut(s)	3498, 3530
FaqI	GGGAC	8 cut(s)	708, 1894, 2322, 2587, 4464, 4602, 4653, 5082
FauNDI	CATATG	1 cut(s)	1910
FbaI	TGATCA	3 cut(s)	1114, 3769, 3892
FblI	GTMKAC	1 cut(s)	4611
FriOI	GRGCYC	6 cut(s)	50, 255, 1509, 2188, 5087, 5132
FspBI	CTAG	8 cut(s)	1341, 1488, 3096, 3302, 4304, 4464, 4956, 5045
GlaI	GCGC	1 cut(s)	308
GsaI	CCCAGC	2 cut(s)	2794, 5103
GsuI	CTGGAG	7 cut(s)	50, 122, 1523, 2138, 4350, 4548, 4968
HgaI	GACGC	4 cut(s)	49, 1286, 2642, 2810
HhaI	GCGC	1 cut(s)	309
Hin1I	GRCGYC	1 cut(s)	41
Hin6I	GCGC	1 cut(s)	307
HinP1I	GCGC	1 cut(s)	307
HincII	GTYRAC	4 cut(s)	485, 2890, 3559, 3883
HindII	GTYRAC	4 cut(s)	485, 2890, 3559, 3883
HindIII	AAGCTT	2 cut(s)	369, 3122
HpaI	GTTAAC	3 cut(s)	2890, 3559, 3883
Hpy166II	GTNNAC	9 cut(s)	485, 903, 2167, 2890, 3559, 3883, 4234, 4612, 4804
Hpy8I	GTNNAC	9 cut(s)	485, 903, 2167, 2890, 3559, 3883, 4234, 4612, 4804
Hpy99I	CGWCG	2 cut(s)	43, 4053
HpyCH4IV	ACGT	7 cut(s)	1290, 1461, 1629, 4530, 4643, 4687, 4744
HpySE526I	ACGT	7 cut(s)	1290, 1461, 1629, 4530, 4643, 4687, 4744
Hsp92I	GRCGYC	1 cut(s)	41
HspAI	GCGC	1 cut(s)	307
Kpn2I	TCCGGA	2 cut(s)	2734, 3481
Ksp22I	TGATCA	3 cut(s)	1114, 3769, 3892
KspAI	GTTAAC	3 cut(s)	2890, 3559, 3883
LguI	GCTCTTC	1 cut(s)	3837
MabI	ACCWGGT	6 cut(s)	434, 2169, 4390, 4648, 4705, 4762
MaeI	CTAG	8 cut(s)	1341, 1488, 3096, 3302, 4304, 4464, 4956, 5045
MaeII	ACGT	7 cut(s)	1290, 1461, 1629, 4530, 4643, 4687, 4744
MfeI	CAATTG	4 cut(s)	322, 686, 2019, 4320
MflI	RGATCY	5 cut(s)	1096, 2764, 3511, 3840, 4094
MlsI	TGGCCA	3 cut(s)	1269, 4121, 4163
MluNI	TGGCCA	3 cut(s)	1269, 4121, 4163
MlyI	GAGTC	5 cut(s)	1071, 1165, 1269, 3247, 4486
MmeI	TCCRAC	5 cut(s)	4485, 4685, 4742, 4799, 5090
Mox20I	TGGCCA	3 cut(s)	1269, 4121, 4163
Mph1103I	ATGCAT	2 cut(s)	421, 1529
MroI	TCCGGA	2 cut(s)	2734, 3481
MroXI	GAANNNNTTC	3 cut(s)	600, 645, 2947
MscI	TGGCCA	3 cut(s)	1269, 4121, 4163
MslI	CAYNNNNRTG	7 cut(s)	1142, 1514, 1646, 3142, 3248, 3596, 3975
Msp20I	TGGCCA	3 cut(s)	1269, 4121, 4163
MspA1I	CMGCKG	3 cut(s)	761, 881, 2116
MspCI	CTTAAG	1 cut(s)	1016
MssI	GTTTAAAC	1 cut(s)	364
MunI	CAATTG	4 cut(s)	322, 686, 2019, 4320
Mva1269I	GAATGC	1 cut(s)	2917
NciI	CCSGG	4 cut(s)	75, 230, 231, 773
NcoI	CCATGG	2 cut(s)	1130, 2247
NdeI	CATATG	1 cut(s)	1910
NheI	GCTAGC	1 cut(s)	3095
NlaIV	GGNNCC	7 cut(s)	14, 1518, 3486, 3842, 4096, 4115, 4317
NmeAIII	GCCGAG	3 cut(s)	69, 145, 4500
NmuCI	GTSAC	5 cut(s)	1168, 1417, 2251, 2857, 4644
NsiI	ATGCAT	2 cut(s)	421, 1529
NspI	RCATGY	5 cut(s)	423, 491, 1907, 3817, 4240
PaeI	GCATGC	1 cut(s)	423
PagI	TCATGA	2 cut(s)	1509, 3735
PciI	ACATGT	2 cut(s)	487, 1903
PciSI	GCTCTTC	1 cut(s)	3837
PctI	GAATGC	1 cut(s)	2917
PdmI	GAANNNNTTC	3 cut(s)	600, 645, 2947
PflFI	GACNNNGTC	1 cut(s)	1319
PflMI	CCANNNNNTGG	1 cut(s)	691
PleI	GAGTC	5 cut(s)	1070, 1165, 1269, 3247, 4485
PmeI	GTTTAAAC	1 cut(s)	364
PpsI	GAGTC	5 cut(s)	1070, 1165, 1269, 3247, 4485
Ppu21I	YACGTR	1 cut(s)	1291
PscI	ACATGT	2 cut(s)	487, 1903
PshAI	GACNNNNGTC	1 cut(s)	4197
PshBI	ATTAAT	1 cut(s)	3657
PsiI	TTATAA	1 cut(s)	1739
Psp124BI	GAGCTC	3 cut(s)	50, 255, 1509
PspFI	CCCAGC	2 cut(s)	2790, 5099
PspN4I	GGNNCC	7 cut(s)	14, 1518, 3486, 3842, 4096, 4115, 4317
PspPI	GGNCC	8 cut(s)	173, 232, 990, 1214, 1925, 2390, 3484, 4199
PstI	CTGCAG	2 cut(s)	640, 5125
PstNI	CAGNNNCTG	1 cut(s)	1166
PsuI	RGATCY	5 cut(s)	1096, 2764, 3511, 3840, 4094
PsyI	GACNNNGTC	1 cut(s)	1319
PvuII	CAGCTG	3 cut(s)	761, 881, 2116
RsaI	GTAC	9 cut(s)	20, 566, 1128, 1250, 3119, 4803, 5030, 5159, 5177
RsaNI	GTAC	9 cut(s)	19, 565, 1127, 1249, 3118, 4802, 5029, 5158, 5176
RseI	CAYNNNNRTG	7 cut(s)	1142, 1514, 1646, 3142, 3248, 3596, 3975
SacI	GAGCTC	3 cut(s)	50, 255, 1509
SapI	GCTCTTC	1 cut(s)	3837
Sau96I	GGNCC	8 cut(s)	173, 232, 990, 1214, 1925, 2390, 3484, 4199
ScaI	AGTACT	1 cut(s)	5177
SchI	GAGTC	5 cut(s)	1071, 1165, 1269, 3247, 4486
SexAI	ACCWGGT	6 cut(s)	434, 2169, 4390, 4648, 4705, 4762
SfcI	CTRYAG	9 cut(s)	636, 1887, 3462, 4633, 4823, 4844, 4885, 4948, 5121
SfiI	GGCCNNNNNGGCC	1 cut(s)	240
SinI	GGWCC	5 cut(s)	173, 1925, 2390, 3484, 4199
SmaI	CCCGGG	1 cut(s)	231
SmiMI	CAYNNNNRTG	7 cut(s)	1142, 1514, 1646, 3142, 3248, 3596, 3975
SmlI	CTYRAG	5 cut(s)	154, 217, 1016, 3456, 4445
SmoI	CTYRAG	5 cut(s)	154, 217, 1016, 3456, 4445
SphI	GCATGC	1 cut(s)	423
SrfI	GCCCGGGC	1 cut(s)	231
SsiI	CCGC	3 cut(s)	150, 3914, 4598
SspI	AATATT	1 cut(s)	1692
SspMI	CTAG	8 cut(s)	1341, 1488, 3096, 3302, 4304, 4464, 4956, 5045
SstI	GAGCTC	3 cut(s)	50, 255, 1509
StyI	CCWWGG	5 cut(s)	1130, 2247, 3311, 3396, 3578
TaiI	ACGT	7 cut(s)	1293, 1464, 1632, 4533, 4646, 4690, 4747
TatI	WGTACW	7 cut(s)	564, 1248, 3117, 4801, 5028, 5157, 5175
TauI	GCSGC	1 cut(s)	153
TscAI	CASTG	9 cut(s)	618, 1584, 1967, 3425, 3689, 4201, 4236, 4333, 5130
TseFI	GTSAC	5 cut(s)	1168, 1417, 2251, 2857, 4644
Tsp45I	GTSAC	5 cut(s)	1168, 1417, 2251, 2857, 4644
TspGWI	ACGGA	4 cut(s)	40, 1093, 2328, 3719
TspMI	CCCGGG	1 cut(s)	229
TspRI	CASTG	9 cut(s)	618, 1584, 1967, 3425, 3689, 4201, 4236, 4333, 5130
Tth111I	GACNNNGTC	1 cut(s)	1319
Van91I	CCANNNNNTGG	1 cut(s)	691
Vha464I	CTTAAG	1 cut(s)	1016
VpaK11BI	GGWCC	5 cut(s)	173, 1925, 2390, 3484, 4199
VspI	ATTAAT	1 cut(s)	3657
XapI	RAATTY	8 cut(s)	850, 1943, 2334, 2416, 2584, 2687, 3518, 4539
XceI	RCATGY	5 cut(s)	423, 491, 1907, 3817, 4240
XcmI	CCANNNNNNNNNTGG	2 cut(s)	535, 1707
XmaI	CCCGGG	1 cut(s)	229
XmiI	GTMKAC	1 cut(s)	4611
XmnI	GAANNNNTTC	3 cut(s)	600, 645, 2947
XspI	CTAG	8 cut(s)	1341, 1488, 3096, 3302, 4304, 4464, 4956, 5045
ZrmI	AGTACT	1 cut(s)	5177
Zsp2I	ATGCAT	2 cut(s)	421, 1529

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.