Rorug02G0241700

Auxilin-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004006
Physical Location & Seq
Reverse (-)
24824077 .. 24826069
1993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0241700.1

Sequence Viewer

Length: 876 bp
ATGGCCACACCAGTGCGAAAGCCTCGCCGCGACACCGCCGACATGCTGACGTGGCAAGAGGTCCCCCGCTCCGACTCTCCCGCCACCGCCTCCTCCGCTCACCGCTCTCACCAGCCGTCGGATGGGATCAGCAAGGTGCTCCACGGCGGTCAGATCACCGAGGAAGAAGCTCAGAGCCTGAACAACAAGAAGCCTTGCTCCGGGTACAAAATGAAGGAGATTTCCGGAAGCGGGATATTCGCGGCTGGAGAAGGCAATGCGGAGCCGGAGCCGGGTCGGGTTTACCAGCAGGCGGTGAAAGGGATCAGTCAAATCTCGTTCAGCGTTGACGAGCACCTGAGCCCGAAGAAGCCGACGAGTATTCCGGAGGTGGCGAAGCAGAAGGAGCTGAGTGGGACTTTGCAGTCGGAATTGGATTCCAAGACTCAGAAGGGGATTTCAAATGCCAAGACCAAGGAGCTCAGTGGAAATGACATCTTTGGCCCTCCCCCGGAAATTGTTCCCCGATCGGTTAATGCTGTTCACACCCACGAGTATAAGCAGAGTAAAGATTCCGGGGAGCCGGCGCCCAGAAATCTCCGCACCTCTGTCAAAGTTTCTAATCCGGCTGGAGGTCAGAGTAAAATCTTGTTCACTGAGGACCCTGTTGTCAAGACTTCCAAGAAAATTCATAACCAGAAGTTTGCAGAGCTGACAGGCAATGACATATTTAAGGGAGATGTTTCTCCGGGATCTGCTGAAAAGCCACTAAGCACGGCTAAGCTGAGAGAAATGAGTGGCAGCAACATCTTTGCTGATGGGAAGGCGGCATCAAGAGACTATCTAGGTGGTGTTCGCAAGCCCCCTGGTGGCGAGAGCAGCATATCATTGGTTTAA

Protein Analysis

291

Amino Acids

31.09

Weight (kDa)

9.2

Isoelectric Point (pI)

51.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4057 PF13266 2 - 289 3.1e-142 Protein of unknown function (DUF4057)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 403, 647
AccB1I GGYRCC 1 cut(s) 565
AccBSI CCGCTC 3 cut(s) 69, 98, 105
AccII CGCG 2 cut(s) 30, 242
AccIII TCCGGA 2 cut(s) 224, 364
AclWI GGATC 3 cut(s) 134, 311, 739
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 666
AcyI GRCGYC 1 cut(s) 566
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 9 cut(s) 118, 122, 200, 231, 272, 292, 490, 611, 848
AgsI TTSAA 1 cut(s) 441
AjiI CACGTC 1 cut(s) 51
AjnI CCWGG 1 cut(s) 844
AleI CACNNNNGTG 1 cut(s) 11
AluBI AGCT 5 cut(s) 170, 388, 460, 691, 763
AluI AGCT 5 cut(s) 170, 388, 460, 691, 763
Alw21I GWGCWC 3 cut(s) 141, 336, 462
Alw26I GTCTC 1 cut(s) 810
AlwI GGATC 3 cut(s) 134, 311, 739
AlwNI CAGNNNCTG 1 cut(s) 178
Aor13HI TCCGGA 2 cut(s) 224, 364
AoxI GGCC 2 cut(s) 3, 481
ApeKI GCWGC 2 cut(s) 780, 858
ApoI RAATTY 1 cut(s) 666
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 1 cut(s) 568
AspS9I GGNCC 3 cut(s) 61, 482, 640
AsuC2I CCSGG 5 cut(s) 202, 273, 491, 556, 729
AsuHPI GGTGA 4 cut(s) 92, 101, 148, 307
AvaII GGWCC 2 cut(s) 61, 640
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 565
BanII GRGCYC 2 cut(s) 344, 462
BauI CACGAG 1 cut(s) 530
Bbv12I GWGCWC 3 cut(s) 141, 336, 462
BbvI GCAGC 2 cut(s) 792, 870
BccI CCATC 2 cut(s) 116, 791
BceAI ACGGC 3 cut(s) 100, 160, 771
BciT130I CCWGG 1 cut(s) 846
BcnI CCSGG 5 cut(s) 202, 273, 491, 556, 729
BcoDI GTCTC 1 cut(s) 810
BfaI CTAG 1 cut(s) 824
BfoI RGCGCY 1 cut(s) 569
BisI GCNGC 5 cut(s) 28, 243, 781, 807, 859
BlpI GCTNAGC 1 cut(s) 759
BlsI GCNGC 5 cut(s) 29, 244, 782, 808, 860
Bme1390I CCNGG 6 cut(s) 202, 273, 491, 556, 729, 846
Bme18I GGWCC 2 cut(s) 61, 640
BmgBI CACGTC 1 cut(s) 51
BmgT120I GGNCC 3 cut(s) 61, 482, 640
BmiI GGNNCC 6 cut(s) 63, 264, 270, 561, 567, 642
BmrFI CCNGG 6 cut(s) 202, 273, 491, 556, 729, 846
BmsI GCATC 1 cut(s) 818
BpmI CTGGAG 2 cut(s) 267, 630
Bpu10I CCTNAGC 1 cut(s) 338
Bpu1102I GCTNAGC 1 cut(s) 759
BpuMI CCSGG 5 cut(s) 202, 273, 491, 556, 729
BsaHI GRCGYC 1 cut(s) 566
BsaJI CCNNGG 6 cut(s) 142, 159, 453, 489, 555, 844
BsaWI WCCGGW 2 cut(s) 224, 364
Bsc4I CCNNNNNNNGG 9 cut(s) 118, 122, 200, 231, 272, 292, 490, 611, 848
Bse118I RCCGGY 1 cut(s) 562
Bse1I ACTGG 1 cut(s) 11
Bse3DI GCAATG 2 cut(s) 262, 706
BseAI TCCGGA 2 cut(s) 224, 364
BseBI CCWGG 1 cut(s) 846
BseDI CCNNGG 6 cut(s) 142, 159, 453, 489, 555, 844
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 9 cut(s) 118, 122, 200, 231, 272, 292, 490, 611, 848
BseMI GCAATG 2 cut(s) 262, 706
BseMII CTCAG 7 cut(s) 185, 329, 380, 440, 475, 627, 755
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 1 cut(s) 82
BseXI GCAGC 2 cut(s) 792, 870
Bsh1236I CGCG 2 cut(s) 30, 242
Bsh1285I CGRYCG 1 cut(s) 509
BshFI GGCC 2 cut(s) 5, 483
BshNI GGYRCC 1 cut(s) 565
BsiEI CGRYCG 1 cut(s) 509
BsiHKAI GWGCWC 3 cut(s) 141, 336, 462
BslFI GGGAC 2 cut(s) 47, 409
BslI CCNNNNNNNGG 9 cut(s) 118, 122, 200, 231, 272, 292, 490, 611, 848
BsmAI GTCTC 1 cut(s) 810
BsmFI GGGAC 2 cut(s) 47, 409
BsnI GGCC 2 cut(s) 5, 483
Bsp1286I GDGCHC 4 cut(s) 141, 336, 344, 462
Bsp13I TCCGGA 2 cut(s) 224, 364
Bsp143I GATC 5 cut(s) 126, 153, 303, 506, 731
Bsp1720I GCTNAGC 1 cut(s) 759
BspANI GGCC 2 cut(s) 5, 483
BspCNI CTCAG 7 cut(s) 184, 330, 381, 439, 474, 628, 756
BspEI TCCGGA 2 cut(s) 224, 364
BspFNI CGCG 2 cut(s) 30, 242
BspLI GGNNCC 6 cut(s) 63, 264, 270, 561, 567, 642
BspPI GGATC 3 cut(s) 134, 311, 739
BspT107I GGYRCC 1 cut(s) 565
BsrBI CCGCTC 3 cut(s) 69, 98, 105
BsrDI GCAATG 2 cut(s) 262, 706
BsrFI RCCGGY 1 cut(s) 562
BsrI ACTGG 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 562
BssECI CCNNGG 6 cut(s) 142, 159, 453, 489, 555, 844
BssMI GATC 5 cut(s) 126, 153, 303, 506, 731
BssNI GRCGYC 1 cut(s) 566
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 530
Bst2UI CCWGG 1 cut(s) 846
BstACI GRCGYC 1 cut(s) 566
BstC8I GCNNGC 3 cut(s) 291, 564, 839
BstDEI CTNAG 9 cut(s) 171, 338, 389, 426, 461, 636, 749, 759, 764
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 1 cut(s) 127
BstFNI CGCG 2 cut(s) 30, 242
BstH2I RGCGCY 1 cut(s) 569
BstHHI GCGC 1 cut(s) 568
BstKTI GATC 5 cut(s) 129, 156, 306, 509, 734
BstMAI GTCTC 1 cut(s) 810
BstMBI GATC 5 cut(s) 126, 153, 303, 506, 731
BstMCI CGRYCG 1 cut(s) 509
BstMWI GCNNNNNNNGC 4 cut(s) 52, 95, 385, 858
BstNI CCWGG 1 cut(s) 846
BstNSI RCATGY 1 cut(s) 46
BstSCI CCNGG 6 cut(s) 200, 271, 489, 554, 727, 844
BstUI CGCG 2 cut(s) 30, 242
BstV1I GCAGC 2 cut(s) 792, 870
BstX2I RGATCY 1 cut(s) 731
BstYI RGATCY 1 cut(s) 731
BsuRI GGCC 2 cut(s) 5, 483
BtgI CCRYGG 1 cut(s) 142
BtrI CACGTC 1 cut(s) 51
BtsCI GGATG 1 cut(s) 127
BtsIMutI CAGTG 3 cut(s) 18, 469, 633
Cac8I GCNNGC 3 cut(s) 291, 564, 839
CaiI CAGNNNCTG 1 cut(s) 178
CfoI GCGC 1 cut(s) 568
Cfr10I RCCGGY 1 cut(s) 562
Cfr13I GGNCC 3 cut(s) 61, 482, 640
Csp6I GTAC 1 cut(s) 205
CviAII CATG 1 cut(s) 43
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 9 cut(s) 171, 338, 389, 426, 461, 636, 749, 759, 764
DinI GGCGCC 1 cut(s) 567
DpnI GATC 5 cut(s) 128, 155, 305, 508, 733
DpnII GATC 5 cut(s) 126, 153, 303, 506, 731
DrdI GACNNNNNNGTC 2 cut(s) 403, 647
DseDI GACNNNNNNGTC 2 cut(s) 403, 647
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 2 cut(s) 344, 462
Eco47I GGWCC 2 cut(s) 61, 640
Eco53kI GAGCTC 1 cut(s) 460
EcoICRI GAGCTC 1 cut(s) 460
EcoO109I RGGNCCY 2 cut(s) 61, 640
EcoRII CCWGG 1 cut(s) 844
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 2 cut(s) 344, 462
EgeI GGCGCC 1 cut(s) 567
EheI GGCGCC 1 cut(s) 567
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 1 cut(s) 46
FaiI YATR 5 cut(s) 44, 537, 672, 707, 863
FaqI GGGAC 2 cut(s) 47, 409
FatI CATG 1 cut(s) 42
FauI CCCGC 3 cut(s) 74, 88, 224
Fnu4HI GCNGC 5 cut(s) 28, 243, 781, 807, 859
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 2 cut(s) 344, 462
Fsp4HI GCNGC 5 cut(s) 28, 243, 781, 807, 859
FspBI CTAG 1 cut(s) 824
GlaI GCGC 1 cut(s) 567
GluI GCNGC 5 cut(s) 28, 243, 781, 807, 859
GsuI CTGGAG 2 cut(s) 267, 630
HaeII RGCGCY 1 cut(s) 569
HaeIII GGCC 2 cut(s) 5, 483
HhaI GCGC 1 cut(s) 568
Hin1I GRCGYC 1 cut(s) 566
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 1 cut(s) 566
HinP1I GCGC 1 cut(s) 566
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 4 cut(s) 74, 416, 424, 551
HphI GGTGA 4 cut(s) 92, 101, 148, 307
Hpy166II GTNNAC 4 cut(s) 283, 328, 523, 633
Hpy188I TCNGA 7 cut(s) 73, 121, 153, 174, 409, 429, 618
Hpy188III TCNNGA 4 cut(s) 225, 365, 652, 813
Hpy8I GTNNAC 4 cut(s) 283, 328, 523, 633
Hpy99I CGWCG 2 cut(s) 121, 358
HpyAV CCTTC 5 cut(s) 208, 245, 376, 424, 796
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 2 cut(s) 403, 686
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 95, 385, 858
HpyF3I CTNAG 9 cut(s) 171, 338, 389, 426, 461, 636, 749, 759, 764
HpySE526I ACGT 1 cut(s) 50
Hsp92I GRCGYC 1 cut(s) 566
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 1 cut(s) 566
KasI GGCGCC 1 cut(s) 565
Kpn2I TCCGGA 2 cut(s) 224, 364
KroI GCCGGC 1 cut(s) 562
KroNI GCCGGC 1 cut(s) 564
Kzo9I GATC 5 cut(s) 126, 153, 303, 506, 731
LmnI GCTCC 8 cut(s) 74, 144, 203, 262, 268, 385, 457, 559
Lsp1109I GCAGC 2 cut(s) 792, 870
LweI GCATC 1 cut(s) 818
MaeI CTAG 1 cut(s) 824
MaeII ACGT 1 cut(s) 50
MalI GATC 5 cut(s) 128, 155, 305, 508, 733
MbiI CCGCTC 3 cut(s) 69, 98, 105
MboI GATC 5 cut(s) 126, 153, 303, 506, 731
MboII GAAGA 2 cut(s) 176, 358
MflI RGATCY 1 cut(s) 731
MhlI GDGCHC 4 cut(s) 141, 336, 344, 462
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 410, 495, 666
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 566
MlyI GAGTC 2 cut(s) 68, 418
MmeI TCCRAC 3 cut(s) 96, 99, 387
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 2 cut(s) 224, 364
MroNI GCCGGC 1 cut(s) 562
MroXI GAANNNNTTC 1 cut(s) 498
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 513, 711, 874
MslI CAYNNNNRTG 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 6 cut(s) 202, 273, 491, 556, 729, 846
MvaI CCWGG 1 cut(s) 846
MvnI CGCG 2 cut(s) 30, 242
MwoI GCNNNNNNNGC 4 cut(s) 52, 95, 385, 858
NaeI GCCGGC 1 cut(s) 564
NarI GGCGCC 1 cut(s) 566
NciI CCSGG 5 cut(s) 202, 273, 491, 556, 729
NdeII GATC 5 cut(s) 126, 153, 303, 506, 731
NgoMIV GCCGGC 1 cut(s) 562
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 6 cut(s) 63, 264, 270, 561, 567, 642
NspI RCATGY 1 cut(s) 46
OliI CACNNNNGTG 1 cut(s) 11
PdiI GCCGGC 1 cut(s) 564
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 2 cut(s) 416, 551
PfoI TCCNGGA 1 cut(s) 727
PkrI GCNGC 5 cut(s) 29, 244, 782, 808, 860
Ple19I CGATCG 1 cut(s) 509
PleI GAGTC 2 cut(s) 68, 418
PluTI GGCGCC 1 cut(s) 569
PpsI GAGTC 2 cut(s) 68, 418
PpuMI RGGWCCY 2 cut(s) 61, 640
Psp124BI GAGCTC 1 cut(s) 462
Psp5II RGGWCCY 2 cut(s) 61, 640
Psp6I CCWGG 1 cut(s) 844
PspGI CCWGG 1 cut(s) 844
PspN4I GGNNCC 6 cut(s) 63, 264, 270, 561, 567, 642
PspPI GGNCC 3 cut(s) 61, 482, 640
PspPPI RGGWCCY 2 cut(s) 61, 640
PstNI CAGNNNCTG 1 cut(s) 178
PsuI RGATCY 1 cut(s) 731
PvuI CGATCG 1 cut(s) 509
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 11
SacI GAGCTC 1 cut(s) 462
SaqAI TTAA 3 cut(s) 513, 711, 874
SatI GCNGC 5 cut(s) 28, 243, 781, 807, 859
Sau3AI GATC 5 cut(s) 126, 153, 303, 506, 731
Sau96I GGNCC 3 cut(s) 61, 482, 640
SchI GAGTC 2 cut(s) 68, 418
ScrFI CCNGG 6 cut(s) 202, 273, 491, 556, 729, 846
SduI GDGCHC 4 cut(s) 141, 336, 344, 462
SfaNI GCATC 1 cut(s) 818
SfoI GGCGCC 1 cut(s) 567
SinI GGWCC 2 cut(s) 61, 640
SmiMI CAYNNNNRTG 1 cut(s) 11
Sse9I AATT 3 cut(s) 410, 495, 666
SspDI GGCGCC 1 cut(s) 565
SspMI CTAG 1 cut(s) 824
SstI GAGCTC 1 cut(s) 462
StyD4I CCNGG 6 cut(s) 200, 271, 489, 554, 727, 844
StyI CCWWGG 1 cut(s) 453
TaiI ACGT 1 cut(s) 53
TasI AATT 3 cut(s) 410, 495, 666
TauI GCSGC 3 cut(s) 30, 245, 809
TfiI GAWTC 2 cut(s) 416, 551
Tru1I TTAA 3 cut(s) 513, 711, 874
Tru9I TTAA 3 cut(s) 513, 711, 874
TscAI CASTG 3 cut(s) 18, 469, 640
TseI GCWGC 2 cut(s) 780, 858
TspDTI ATGAA 2 cut(s) 227, 659
TspRI CASTG 3 cut(s) 18, 469, 640
VpaK11BI GGWCC 2 cut(s) 61, 640
XapI RAATTY 1 cut(s) 666
XceI RCATGY 1 cut(s) 46
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.