Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0003401

Physical Location & Seq

Genomic Coordinates Forward (+)

33410259 .. 33419613

Gene Structure 9355 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0299500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4650 bp

ATGTCATCGAGCAATTTGAAAGGGTTCATATTGGCCGTGGTCTCTAGCGCCTTCATAGGCTCCAGCTTCATCATCAAGAAGAAGGGTCTCCGCCGAGCCGGCAACAATGGAACTCGTGCCAGTTCTGGAGGATATGGGTATCTGCGGGAGCCTCTATGGTGGATAGGAATGGTCACAATGGTTGTTGGAGAGATAGCAAATTTTGTAGCATACATATACGCACCTGCCGGGCTTGTCACGCCGCTTGGTGCTTTGAGTATCATTGTTAGTGCGGTGTTAGCGCATTTCCTGTTAAAGGAGAAACTCCAGAAAATGGGAATGTTGGGCTGCCTTTTATGCATAGTGGGTTCGGTTGTGATTGTACTCCATGCACCCGAAGAGCAGACTCTCACTTCCGTCCAACAAATCTGGGATTTAGCAATACAACCAGCTTTCCTTTTGTATACAGCCTCAGTGATAGCTGTAACTCTAGTCCTTATCTTGTATTGTTCCCCCCGCTATGGCCAAACCAATATACTGATTTATATAGGAATATGCTCAATGATTGGATCATTGACTGTCATGAGCATAAAAGCAATTGGCATTGCAATAAAACTCACAATAGAGGGCACAAACCAGGCAGTATACTTCCAAACATGGATTTTTGCAATGGTTGCAGTTACATGTATCATCACTCAATTAAATTATCTAAATATGGCATTGGACACTTTCAACACAGCAATCGTTTCTCCCATCTATTATGCTATGTTCACATCTTTCACAATACTTGCGAGTGCAATAATGTTTAAGGATTATTCTGGTCAAAGTGCGAGCAGCATAGCTTCAGAACTTTGTGGATTCATCACTGTGTTATCTGGGACTGCTATATTGCATAGTACAAGAGAACCAGATCCACCTTTACTTACAGATTTGTATACACCATTATCTCCTAAAGTATCGTGGTACATCCAAAGCAATGGAGAATGGAAGCAGAAGGATGAAGACGGTTCAGCCCCCAATTTCATCACACCAGGGATATCCACTTGGGACTGTAAAAATCGGTCGTGCTCGCGATACCAAATGGACCATTGCATGAAAAGGACTCTGTCTTTGAAGCAGCCGCCGATTTTGGCTCAGTCCAAACCCTCCTCCTATTCGTCGAAACCGAACCACTTCCGCTTCCTCCTCTACTCCAAGAAGCTCGGCGCCCTACCGTCGCTCAGCCTCCGGTCATGGCCGCAGTCGCAGAGTCATCGTCCGAGTTGCCGAGTCAACTCGGTTCGGAGCGAGTCGTTGTCCGACGGCGGCGTGGCGGAGAAGAAGGAAGAAGCAAAGGCGGCGCCGAAGAGGAAGGTGGGGAAGAGGACGGACTTGAAGAAGATTATGATACTGGGCGCCGGGCCGATTGTGATCGGGCAAGCTTGCGAGTTCGATTACTCGGGGACTCAAGCTTGTAAAGCTTTGAAGGAGGACGGATACGAGGTCGTTTTGATCAATTCCAACCCGGCTACGATCATGACCGACCCGGATTTGGCCGATAGGACCTACATTACGCCCATGACGCCGGAGTTGGTGGAGCAGGTGCTCGAAAAGGAGCGGCCGGACGCTCTTCTGCCGACCATGGGAGGCCAGACGGCGTTGAACCTGGCGGTGGCGTTGGCCGAAAGCGGCGCGCTGGAGAAGTATGGTGTGGAGTTGATTGGAGCCAAGCTGGAGGCCATTAAGAAAGCTGAGGATAGAGAGCTGTTCAAGCAGGCGATGAAGAACATTGGGGTTGAAACGCCGCCGTCTGGAATTGCCAACACCTTGGAGGAATGTGTGGAAATTGCGAAAGAGATTGGTGAGTTTCCCTTGATCATTAGGCCAGCTTTTACGTTGGGAGGGACGGGAGGTGGTATTGCATATAATAAGGAGGAATTTGAGACTATTTGTAAGGCCGGGCTGGCGGCGAGTTTGACCTCTCAGGTTTTGGTGGAGAAGTCACTGTTGGGGTGGAAGGAATATGAGCTTGAGGTTATGAGGGACTTGGCAGATAATGTGGTTATTATTTGCTCCATTGAGAATATTGACCCGATGGGGGTTCATACGGGAGACTCTATCACCGTGGCGCCTGCCCAGACTTTAACTGATAAGGAGTATCAGCGGCTGAGGGACTATTCGATAGCTATAATAAGAGAGATTGGAGTGGAATGTGGTGGTTCTAATGTGCAGTTTGCTGTGAATCCTCAAGACGGGGAGGTTATGGTGATTGAGATGAATCCAAGGGTGTCACGGTCCTCAGCCCTTGCATCCAAAGCAACTGGTTTTCCGATAGCGAAAATGGCTGCTAAGTTGTCAGTTGGATATTCCTTGGATCAAATTCCCAATGACATTACAAAGAAGACACCGGCTAGTTTTGAGCCTTCCATAGACTATGTGGTAACAAAGATTCCCCGGTTTGCTTTTGAAAAGTTTCCTGGTTCAGAGCCAATACTGACGACCCAGATGAAATCTGTCGGTGAGTCAATGGCACTAGGGCGTACATTTCAAGAATCCTTTCAGAAAGCAGTTCGATCTTTGGAATGTGGTTTCTCTGGATGGGGCTGTGGGAAAATTAAGGAACTAGACTGGGATTGGGACCAATTGAAATATAGTCTCCGAGTCCCTAACCCAGATCGTATCCATGCTGTGTATGCTGCAATGAAGAAGGGAATGAAGATAGATGAAATTCATGAGCTGAGTTTCATTGACAAATGGTTCCTCACACAGCTTAAAGAATTGGTGGATGTAGAGCAATTCCTCCTGGCTAGAAACTTGTCTGGTCTAACAAAGGATGACTTCTATGAAGTGAAAAGAAGAGGCTTCAGTGACAAACAGATAGCTTTTGCCACTAAATCCTCTGAAAATGAAGTGCGGTTGAAGAGATTATCATTGGGTGTCACTCCAGCATATAAGCGGGTAGATACGTGTGCCGCAGAGTTTGAGGCCAATACTCCTTATATGTACTCGTCTTATGACTTTGAGTGTGAATCCGCTCCCACCCATAAAAAGAAGGTTTTGATTTTAGGTGGAGGACCAAATCGAATTGGTCAGGGTATTGAATTTGACTACTGCTGTTGCCATACATCCTTTGCCCTTCAGAAAGCAGGATATGAGACAATCATGATGAATTCAAATCCTGAAACAGTATCTACTGATTATGACACAAGTGACCGTTTATATTTTGAGCCCCTGACTGTTGAAGATGTGCTGAACATAATTGATTTGGAAAGACCTGATGGGATCATTGTGCAGTTTGGAGGTCAAACACCTCTGAAGCTAGCACTTCCCCTCCAGCGGTATTTAGATGAAAACAAGCCTAGATGTGCAAGTGGTGCTGGACATGTTCGCATTTGGGGTACCACACCTGACTCCATAGATGCTGCTGAGGACAGGGAGAAGTTCAATGCAATTCTGAACGAGTTAAAGATTGAACAGCCAAAAGGAGGAATTGCCAAGAGTGAAGCAGATGCTCTTGCCATTGCCACAAACATTGGCTACCCTGTCGTTGTCCGACCCTCCTATGTATTGGGCGGCCGTGCCATGGAGATAGTATATAGTGATGAAAAACTTGTGACCTACCTAGAAACTGCTGTTGAGGTGGACCCGGAATGTCCTGTATTGATTGACAGGTATCTATCCGATGCCATTGAGATTGATGTTGATGCACTTGCTGACTCTCATGGGAATGTCGTCATTGGTGGGATAATGGAGCACATTGAGCAGGCTGGGGTCCATTCTGGTGATTCTGCTTGCTCGATTCCGACAAAAACCATCCCAGAGTCTTGCTTGAATACAATCAGGTCATGGACTATAAAATTGGCTAAGAGGCTGAATGTATGTGGGCTGATGAACTGTCAGTATGCAATTACAATGTCTGGAGATGTTTATTTACTCGAGGCAAATCCTCGTGCTTCCCGTACGGTCCCTTTTGTGTCCAAGGCAATTGGACATCCGTTGGCTAAATATGCTTCCCTGGTTATGTCTGGGAAGTCTCTTCATGATCTTGGTTTCACAAAAGAAGTCATTCCTGCACATATGTCAGTGAAAGAAGCTGTTCTTCCATTTGAGAAGTTCCAAGGTTGTGATGTATTGCTTGGGCCTGAGATGCGTAGCACTGGTGAGGTGATGGGTATTGATTTTGAGTTTCCAATTGCATTTGCCAAGGCTCAGATTGCTGCTGGGCAGTCGCTACCACTTTCAGGCACTGTGTTTATGAGCTTAAATGACTTGACCAAACCCCATCTTGAGAGAATTGCAAAGGCATTTTTAGCTCTTGGATTGAAGATTGTTTCAACTTCAGGAACAGCTCATGTTCTTGAACTAGCGAAAATTCCGGTGGAACGAGTGCTGAAGCTACATGAGGGGAGGCCTAATGCTGGTGACATGGTTGCTAATGGGCAAATCCAGTTGATGGTGATTACAAGTTCTGGTGATGCACTCGATCAGATTGATGGACGGCAGCTAAGGAGGACGGCCCTTGCTTACAAGATTCCAGTAATAACAACAGTTGCTGGAGCTTTGGCAACTGCAGAAGCAATAAAGAGCTTAAAGACCAGCTCCATTAAGATGATTGCACTTCAGGACTTCTTTGATGACGAGAATAAAGCTGTCAGTGATAAAAAGTTGCAGTCTTCCTCCGTCTCTCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0080090 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

1549

Amino Acids

169.24

Weight (kDa)

5.98

Isoelectric Point (pI)

38.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mg_trans_NIPA	PF05653	5 - 295	4.2e-131	Magnesium transporter NIPA
EamA	PF00892	55 - 121	1.6e-06	EamA-like transporter family
CPSase_L_D1	PF25596	451 - 568	1.9e-48	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	572 - 779	1.7e-72	Carbamoyl-phosphate synthase L chain, ATP binding domain
CPSase_L_D3	PF02787	871 - 949	2.6e-26	Carbamoyl-phosphate synthetase large chain, oligomerisation domain
CPSase_L_D1	PF25596	1002 - 1126	1.5e-45	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	1130 - 1333	1.8e-37	Carbamoyl-phosphate synthase L chain, ATP binding domain
Dala_Dala_lig_C	PF07478	1157 - 1302	5.7e-07	D-ala D-ala ligase C-terminus
MGS	PF02142	1416 - 1501	3.2e-19	MGS-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003401)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79640 AT1G79640 AT1G79640 AT1G79640 AT1G79640 AT1G79640
fragaria_vesca	FvH4_6g28050 FvH4_6g28050 FvH4_6g28050 FvH4_6g28050 FvH4_6g28050 FvH4_6g28050 FvH4_6g28050
malus_domestica	MD09G1229900.v1.1 MD17G1228700.v1.1
prunus_persica	Prupe.3G106100_v2.0.a1 Prupe.3G106100_v2.0.a1
pyrus_communis	pycom09g14840 pycom17g23120
rosa_chinensis	RchiOBHm_Chr2g0131781 RchiOBHm_Chr7g0213591
rosa_laevigata	RLG00000019211
rosa_multiflora	Rmu_sc0002589.1_g000040 Rmu_sc0009880.1_g000006
rosa_roxburghii	Rroxscaffold_2G00112600
rosa_rugosa	Rorug02G0299500 Rorug02G0299600 Rorug02G0299700 Rorug02G0299800
rosa_samantha	Rh1BG109800 Rh2AG352000 Rh2BG359100 Rh2CG337600 Rh2DG376500 Rh5CG277400
rosa_wichuraiana	Rw2G028610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	232, 1549
Acc36I	ACCTGC	2 cut(s)	232, 1549
Acc65I	GGTACC	1 cut(s)	3356
AccB1I	GGYRCC	5 cut(s)	1184, 1318, 1373, 2086, 3356
AccB7I	CCANNNNNTGG	2 cut(s)	313, 4411
AccBSI	CCGCTC	2 cut(s)	1576, 2993
AccI	GTMKAC	3 cut(s)	443, 624, 914
AccII	CGCG	2 cut(s)	1051, 1652
AclWI	GGATC	4 cut(s)	556, 884, 2340, 3248
AcoI	YGGCCR	7 cut(s)	33, 502, 1214, 1512, 1577, 1638, 3532
AcsI	RAATTY	7 cut(s)	199, 1895, 2337, 2685, 3059, 3127, 4329
AcuI	CTGAAG	7 cut(s)	807, 2806, 3080, 3293, 4281, 4370, 4562
AcyI	GRCGYC	5 cut(s)	1185, 1319, 1374, 1541, 2087
AfaI	GTAC	7 cut(s)	363, 877, 944, 2500, 2963, 3358, 3919
AflIII	ACRYGT	3 cut(s)	662, 2924, 3340
AjnI	CCWGG	6 cut(s)	615, 1009, 1623, 2434, 2760, 3972
AjuI	GAANNNNNNNTTGG	2 cut(s)	1949, 1981
Alw21I	GWGCWC	3 cut(s)	1049, 1566, 3714
Alw26I	GTCTC	8 cut(s)	46, 92, 1895, 2064, 2618, 3107, 3996, 4645
AlwI	GGATC	4 cut(s)	556, 884, 2340, 3248
AlwNI	CAGNNNCTG	3 cut(s)	2125, 4205, 4511
Ama87I	CYCGRG	2 cut(s)	1417, 3893
ApeKI	GCWGC	8 cut(s)	327, 813, 1096, 2303, 2654, 3380, 4175, 4459
ApoI	RAATTY	7 cut(s)	199, 1895, 2337, 2685, 3059, 3127, 4329
ArsI	GACNNNNNNTTYG	2 cut(s)	1072, 1104
Asp700I	GAANNNNTTC	6 cut(s)	23, 1151, 2430, 2514, 4023, 4053
Asp718I	GGTACC	1 cut(s)	3356
AspLEI	GCGC	8 cut(s)	50, 283, 1187, 1321, 1376, 1652, 1654, 2089
AsuC2I	CCSGG	7 cut(s)	229, 1378, 1484, 1505, 1918, 2413, 3605
AsuNHI	GCTAGC	1 cut(s)	3277
AvaI	CYCGRG	2 cut(s)	1417, 3893
AvaII	GGWCC	8 cut(s)	1063, 1521, 2253, 2596, 3032, 3601, 3730, 3922
BaeGI	GKGCMC	1 cut(s)	611
BalI	TGGCCA	1 cut(s)	504
BanI	GGYRCC	5 cut(s)	1184, 1318, 1373, 2086, 3356
BanII	GRGCYC	1 cut(s)	3189
BauI	CACGAG	2 cut(s)	114, 3906
BbsI	GAAGAC	3 cut(s)	987, 2366, 4623
Bbv12I	GWGCWC	3 cut(s)	1049, 1566, 3714
BbvCI	CCTCAGC	4 cut(s)	1710, 2126, 2257, 3384
BbvI	GCAGC	8 cut(s)	314, 825, 1108, 2290, 2641, 3367, 4162, 4471
BccI	CCATC	9 cut(s)	740, 2047, 2550, 3230, 3779, 4120, 4248, 4405, 4445
BceAI	ACGGC	7 cut(s)	20, 1297, 1629, 1750, 3519, 4472, 4488
BcgI	CGANNNNNNTGC	2 cut(s)	1214, 1248
BciT130I	CCWGG	6 cut(s)	617, 1011, 1625, 2436, 2762, 3974
BciVI	GTATCC	2 cut(s)	1448, 2648
BclI	TGATCA	2 cut(s)	1470, 1833
BcnI	CCSGG	7 cut(s)	229, 1378, 1484, 1505, 1918, 2413, 3605
BcoDI	GTCTC	8 cut(s)	46, 92, 1895, 2064, 2618, 3107, 3996, 4645
BfmI	CTRYAG	1 cut(s)	4527
BfoI	RGCGCY	5 cut(s)	51, 1188, 1322, 1377, 2090
BfuAI	ACCTGC	2 cut(s)	232, 1549
BfuI	GTATCC	2 cut(s)	1448, 2648
BglI	GCCNNNNNGGC	2 cut(s)	99, 1922
BlpI	GCTNAGC	1 cut(s)	1199
Bme18I	GGWCC	8 cut(s)	1063, 1521, 2253, 2596, 3032, 3601, 3730, 3922
BmeT110I	CYCGRG	2 cut(s)	1417, 3893
BmrI	ACTGGG	2 cut(s)	1379, 2596
BmsI	GCATC	7 cut(s)	2276, 3367, 3457, 3631, 3652, 4095, 4423
BmtI	GCTAGC	1 cut(s)	3281
BmuI	ACTGGG	2 cut(s)	1379, 2596
BpiI	GAAGAC	3 cut(s)	987, 2366, 4623
BpmI	CTGGAG	9 cut(s)	46, 147, 290, 1676, 1712, 2886, 3275, 3897, 4533
Bpu10I	CCTNAGC	5 cut(s)	1710, 2126, 2257, 3384, 4463
Bpu1102I	GCTNAGC	1 cut(s)	1199
BpuEI	CTTGAG	4 cut(s)	1410, 2009, 2190, 4265
BpuMI	CCSGG	7 cut(s)	229, 1378, 1484, 1505, 1918, 2413, 3605
BsaAI	YACGTR	1 cut(s)	2925
BsaBI	GATNNNNATC	1 cut(s)	1388
BsaHI	GRCGYC	5 cut(s)	1185, 1319, 1374, 1541, 2087
BsaI	GGTCTC	2 cut(s)	46, 92
BsaWI	WCCGGW	2 cut(s)	1206, 4333
BsaXI	ACNNNNNCTCC	2 cut(s)	3273, 3303
Bse118I	RCCGGY	2 cut(s)	98, 2365
Bse1I	ACTGG	7 cut(s)	120, 1374, 2284, 2591, 4120, 4405, 4493
Bse3DI	GCAATG	6 cut(s)	582, 654, 961, 1066, 2664, 3477
Bse8I	GATNNNNATC	1 cut(s)	1388
BseBI	CCWGG	6 cut(s)	617, 1011, 1625, 2436, 2762, 3974
BseGI	GGATG	9 cut(s)	945, 982, 2267, 2561, 2749, 2797, 3083, 3771, 3949
BseJI	GATNNNNATC	1 cut(s)	1388
BseMI	GCAATG	6 cut(s)	582, 654, 961, 1066, 2664, 3477
BseNI	ACTGG	7 cut(s)	120, 1374, 2284, 2591, 4120, 4405, 4493
BsePI	GCGCGC	1 cut(s)	1650
BseRI	GAGGAG	2 cut(s)	1117, 1154
BseSI	GKGCMC	1 cut(s)	611
BseX3I	CGGCCG	2 cut(s)	1577, 3532
BseXI	GCAGC	8 cut(s)	314, 825, 1108, 2290, 2641, 3367, 4162, 4471
BseYI	CCCAGC	2 cut(s)	3725, 4178
BsgI	GTGCAG	3 cut(s)	2207, 3269, 4014
Bsh1236I	CGCG	2 cut(s)	1051, 1652
Bsh1285I	CGRYCG	3 cut(s)	1043, 1580, 3535
BshNI	GGYRCC	5 cut(s)	1184, 1318, 1373, 2086, 3356
BsiEI	CGRYCG	3 cut(s)	1043, 1580, 3535
BsiHKAI	GWGCWC	3 cut(s)	1049, 1566, 3714
BsiHKCI	CYCGRG	2 cut(s)	1417, 3893
BsiWI	CGTACG	1 cut(s)	3917
BslFI	GGGAC	9 cut(s)	871, 1040, 1435, 1876, 2015, 2144, 2606, 2609, 3908
BsmAI	GTCTC	8 cut(s)	46, 92, 1895, 2064, 2618, 3107, 3996, 4645
BsmBI	CGTCTC	1 cut(s)	4645
BsmFI	GGGAC	9 cut(s)	871, 1040, 1435, 1876, 2015, 2144, 2606, 2609, 3908
Bso31I	GGTCTC	2 cut(s)	46, 92
BsoBI	CYCGRG	2 cut(s)	1417, 3893
Bsp1286I	GDGCHC	5 cut(s)	611, 1049, 1566, 3189, 3714
Bsp1720I	GCTNAGC	1 cut(s)	1199
Bsp19I	CCATGG	2 cut(s)	1599, 3540
Bsp68I	TCGCGA	1 cut(s)	1051
BspFNI	CGCG	2 cut(s)	1051, 1652
BspHI	TCATGA	5 cut(s)	561, 1494, 2689, 3120, 3997
BspMAI	CTGCAG	1 cut(s)	4531
BspMI	ACCTGC	2 cut(s)	232, 1549
BspOI	GCTAGC	1 cut(s)	3281
BspPI	GGATC	4 cut(s)	556, 884, 2340, 3248
BspQI	GCTCTTC	2 cut(s)	372, 1593
BspT107I	GGYRCC	5 cut(s)	1184, 1318, 1373, 2086, 3356
BspTNI	GGTCTC	2 cut(s)	46, 92
BsrBI	CCGCTC	2 cut(s)	1576, 2993
BsrDI	GCAATG	6 cut(s)	582, 654, 961, 1066, 2664, 3477
BsrFI	RCCGGY	2 cut(s)	98, 2365
BsrI	ACTGG	7 cut(s)	120, 1374, 2284, 2591, 4120, 4405, 4493
BssAI	RCCGGY	2 cut(s)	98, 2365
BssHII	GCGCGC	1 cut(s)	1650
BssNAI	GTATAC	3 cut(s)	444, 625, 915
BssNI	GRCGYC	5 cut(s)	1185, 1319, 1374, 1541, 2087
BssSI	CACGAG	2 cut(s)	114, 3906
BssT1I	CCWWGG	8 cut(s)	1599, 1785, 2240, 2328, 3540, 3936, 4075, 4161
Bst1107I	GTATAC	3 cut(s)	444, 625, 915
Bst2BI	CACGAG	2 cut(s)	114, 3906
Bst2UI	CCWGG	6 cut(s)	617, 1011, 1625, 2436, 2762, 3974
Bst6I	CTCTTC	7 cut(s)	372, 1319, 1334, 1593, 2809, 2873, 3999
BstACI	GRCGYC	5 cut(s)	1185, 1319, 1374, 1541, 2087
BstAPI	GCANNNNNTGC	2 cut(s)	653, 3332
BstBAI	YACGTR	1 cut(s)	2925
BstDSI	CCRYGG	4 cut(s)	36, 1599, 2082, 3540
BstENI	CCTNNNNNAGG	1 cut(s)	293
BstF5I	GGATG	9 cut(s)	945, 982, 2267, 2561, 2749, 2797, 3083, 3771, 3949
BstFNI	CGCG	2 cut(s)	1051, 1652
BstH2I	RGCGCY	5 cut(s)	51, 1188, 1322, 1377, 2090
BstHHI	GCGC	8 cut(s)	50, 283, 1187, 1321, 1376, 1652, 1654, 2089
BstMAI	GTCTC	8 cut(s)	46, 92, 1895, 2064, 2618, 3107, 3996, 4645
BstMCI	CGRYCG	3 cut(s)	1043, 1580, 3535
BstNI	CCWGG	6 cut(s)	617, 1011, 1625, 2436, 2762, 3974
BstNSI	RCATGY	2 cut(s)	666, 3344
BstSFI	CTRYAG	1 cut(s)	4527
BstSLI	GKGCMC	1 cut(s)	611
BstUI	CGCG	2 cut(s)	1051, 1652
BstV1I	GCAGC	8 cut(s)	314, 825, 1108, 2290, 2641, 3367, 4162, 4471
BstV2I	GAAGAC	3 cut(s)	987, 2366, 4623
BstX2I	RGATCY	1 cut(s)	889
BstXI	CCANNNNNNTGG	2 cut(s)	956, 1786
BstYI	RGATCY	1 cut(s)	889
BstZ17I	GTATAC	3 cut(s)	444, 625, 915
BstZI	CGGCCG	2 cut(s)	1577, 3532
BsuI	GTATCC	2 cut(s)	1448, 2648
BtgI	CCRYGG	4 cut(s)	36, 1599, 2082, 3540
BtgZI	GCGATG	1 cut(s)	1751
BtsCI	GGATG	9 cut(s)	945, 982, 2267, 2561, 2749, 2797, 3083, 3771, 3949
BtsIMutI	CAGTG	8 cut(s)	459, 843, 1961, 2830, 4047, 4113, 4203, 4618
BtuMI	TCGCGA	1 cut(s)	1051
BveI	ACCTGC	2 cut(s)	232, 1549
CaiI	CAGNNNCTG	3 cut(s)	2125, 4205, 4511
CciI	TCATGA	5 cut(s)	561, 1494, 2689, 3120, 3997
CfoI	GCGC	8 cut(s)	50, 283, 1187, 1321, 1376, 1652, 1654, 2089
Cfr10I	RCCGGY	2 cut(s)	98, 2365
CseI	GACGC	2 cut(s)	1549, 1592
Csp6I	GTAC	7 cut(s)	362, 876, 943, 2499, 2962, 3357, 3918
CspCI	CAANNNNNGTGG	2 cut(s)	3472, 3507
CviQI	GTAC	7 cut(s)	362, 876, 943, 2499, 2962, 3357, 3918
DinI	GGCGCC	4 cut(s)	1186, 1320, 1375, 2088
EaeI	YGGCCR	7 cut(s)	33, 502, 1214, 1512, 1577, 1638, 3532
EagI	CGGCCG	2 cut(s)	1577, 3532
Eam1104I	CTCTTC	7 cut(s)	372, 1319, 1334, 1593, 2809, 2873, 3999
EarI	CTCTTC	7 cut(s)	372, 1319, 1334, 1593, 2809, 2873, 3999
EciI	GGCGGA	2 cut(s)	80, 1307
EclXI	CGGCCG	2 cut(s)	1577, 3532
Eco130I	CCWWGG	8 cut(s)	1599, 1785, 2240, 2328, 3540, 3936, 4075, 4161
Eco147I	AGGCCT	1 cut(s)	4369
Eco24I	GRGCYC	1 cut(s)	3189
Eco31I	GGTCTC	2 cut(s)	46, 92
Eco32I	GATATC	1 cut(s)	1017
Eco47I	GGWCC	8 cut(s)	1063, 1521, 2253, 2596, 3032, 3601, 3730, 3922
Eco52I	CGGCCG	2 cut(s)	1577, 3532
Eco57I	CTGAAG	7 cut(s)	807, 2806, 3080, 3293, 4281, 4370, 4562
Eco88I	CYCGRG	2 cut(s)	1417, 3893
EcoNI	CCTNNNNNAGG	1 cut(s)	293
EcoO109I	RGGNCCY	1 cut(s)	1521
EcoRI	GAATTC	1 cut(s)	3127
EcoRII	CCWGG	6 cut(s)	615, 1009, 1623, 2434, 2760, 3972
EcoRV	GATATC	1 cut(s)	1017
EcoT14I	CCWWGG	8 cut(s)	1599, 1785, 2240, 2328, 3540, 3936, 4075, 4161
EcoT22I	ATGCAT	1 cut(s)	341
EcoT38I	GRGCYC	1 cut(s)	3189
EgeI	GGCGCC	4 cut(s)	1186, 1320, 1375, 2088
EheI	GGCGCC	4 cut(s)	1186, 1320, 1375, 2088
ErhI	CCWWGG	8 cut(s)	1599, 1785, 2240, 2328, 3540, 3936, 4075, 4161
Esp3I	CGTCTC	1 cut(s)	4645
FalI	AAGNNNNNCTT	4 cut(s)	2780, 2812, 4041, 4073
FaqI	GGGAC	9 cut(s)	871, 1040, 1435, 1876, 2015, 2144, 2606, 2609, 3908
FauI	CCCGC	3 cut(s)	138, 503, 2907
FauNDI	CATATG	1 cut(s)	4035
FbaI	TGATCA	2 cut(s)	1470, 1833
FblI	GTMKAC	3 cut(s)	443, 624, 914
FokI	GGATG	9 cut(s)	932, 989, 2254, 2568, 2756, 2804, 3070, 3758, 3936
FriOI	GRGCYC	1 cut(s)	3189
GlaI	GCGC	8 cut(s)	49, 282, 1186, 1320, 1375, 1651, 1653, 2088
GsaI	CCCAGC	2 cut(s)	3729, 4182
GsuI	CTGGAG	9 cut(s)	46, 147, 290, 1676, 1712, 2886, 3275, 3897, 4533
HaeII	RGCGCY	5 cut(s)	51, 1188, 1322, 1377, 2090
HgaI	GACGC	2 cut(s)	1549, 1592
HhaI	GCGC	8 cut(s)	50, 283, 1187, 1321, 1376, 1652, 1654, 2089
Hin1I	GRCGYC	5 cut(s)	1185, 1319, 1374, 1541, 2087
Hin6I	GCGC	8 cut(s)	48, 281, 1185, 1319, 1374, 1650, 1652, 2087
HinP1I	GCGC	8 cut(s)	48, 281, 1185, 1319, 1374, 1650, 1652, 2087
HincII	GTYRAC	1 cut(s)	1252
HindII	GTYRAC	1 cut(s)	1252
HindIII	AAGCTT	3 cut(s)	1398, 1428, 1437
Hpy166II	GTNNAC	6 cut(s)	444, 625, 750, 915, 1252, 3601
Hpy8I	GTNNAC	6 cut(s)	444, 625, 750, 915, 1252, 3601
Hpy99I	CGWCG	3 cut(s)	1141, 1198, 1283
HpyCH4IV	ACGT	2 cut(s)	1853, 2924
HpySE526I	ACGT	2 cut(s)	1853, 2924
Hsp92I	GRCGYC	5 cut(s)	1185, 1319, 1374, 1541, 2087
HspAI	GCGC	8 cut(s)	48, 281, 1185, 1319, 1374, 1650, 1652, 2087
KasI	GGCGCC	4 cut(s)	1184, 1318, 1373, 2086
KpnI	GGTACC	1 cut(s)	3360
KroI	GCCGGC	1 cut(s)	98
KroNI	GCCGGC	1 cut(s)	100
Ksp22I	TGATCA	2 cut(s)	1470, 1833
LguI	GCTCTTC	2 cut(s)	372, 1593
Lsp1109I	GCAGC	8 cut(s)	314, 825, 1108, 2290, 2641, 3367, 4162, 4471
LweI	GCATC	7 cut(s)	2276, 3367, 3457, 3631, 3652, 4095, 4423
MaeII	ACGT	2 cut(s)	1853, 2924
MbiI	CCGCTC	2 cut(s)	1576, 2993
MfeI	CAATTG	4 cut(s)	576, 2600, 3942, 4149
MflI	RGATCY	1 cut(s)	889
MhlI	GDGCHC	5 cut(s)	611, 1049, 1566, 3189, 3714
MlsI	TGGCCA	1 cut(s)	504
MluNI	TGGCCA	1 cut(s)	504
Mly113I	GGCGCC	4 cut(s)	1185, 1319, 1374, 2087
MmeI	TCCRAC	7 cut(s)	166, 424, 1302, 1503, 2299, 3535, 3785
Mox20I	TGGCCA	1 cut(s)	504
Mph1103I	ATGCAT	1 cut(s)	341
MroNI	GCCGGC	1 cut(s)	98
MroXI	GAANNNNTTC	6 cut(s)	23, 1151, 2430, 2514, 4023, 4053
MscI	TGGCCA	1 cut(s)	504
MslI	CAYNNNNRTG	4 cut(s)	845, 3684, 3738, 4565
Msp20I	TGGCCA	1 cut(s)	504
MspA1I	CMGCKG	2 cut(s)	2122, 3295
MunI	CAATTG	4 cut(s)	576, 2600, 3942, 4149
MvaI	CCWGG	6 cut(s)	617, 1011, 1625, 2436, 2762, 3974
MvnI	CGCG	2 cut(s)	1051, 1652
NaeI	GCCGGC	1 cut(s)	100
NarI	GGCGCC	4 cut(s)	1185, 1319, 1374, 2087
NciI	CCSGG	7 cut(s)	229, 1378, 1484, 1505, 1918, 2413, 3605
NcoI	CCATGG	2 cut(s)	1599, 3540
NdeI	CATATG	1 cut(s)	4035
NgoMIV	GCCGGC	1 cut(s)	98
NheI	GCTAGC	1 cut(s)	3277
NmeAIII	GCCGAG	3 cut(s)	119, 1161, 1271
NmuCI	GTSAC	9 cut(s)	172, 235, 1959, 2247, 2825, 2896, 3167, 3571, 4379
NruI	TCGCGA	1 cut(s)	1051
NsiI	ATGCAT	1 cut(s)	341
NspI	RCATGY	2 cut(s)	666, 3344
PaeR7I	CTCGAG	1 cut(s)	3893
PagI	TCATGA	5 cut(s)	561, 1494, 2689, 3120, 3997
PaqCI	CACCTGC	2 cut(s)	232, 1549
PauI	GCGCGC	1 cut(s)	1650
PceI	AGGCCT	1 cut(s)	4369
PciI	ACATGT	2 cut(s)	662, 3340
PciSI	GCTCTTC	2 cut(s)	372, 1593
PcsI	WCGNNNNNNNCGW	2 cut(s)	1142, 3913
PdiI	GCCGGC	1 cut(s)	100
PdmI	GAANNNNTTC	6 cut(s)	23, 1151, 2430, 2514, 4023, 4053
PfeI	GAWTC	9 cut(s)	837, 2200, 2236, 2407, 2510, 2987, 3743, 3757, 4489
Pfl23II	CGTACG	1 cut(s)	3917
PflFI	GACNNNGTC	1 cut(s)	1084
PflMI	CCANNNNNTGG	2 cut(s)	313, 4411
PluTI	GGCGCC	4 cut(s)	1188, 1322, 1377, 2090
Ppu21I	YACGTR	1 cut(s)	2925
PpuMI	RGGWCCY	1 cut(s)	1521
PscI	ACATGT	2 cut(s)	662, 3340
Psp5II	RGGWCCY	1 cut(s)	1521
Psp6I	CCWGG	6 cut(s)	615, 1009, 1623, 2434, 2760, 3972
PspFI	CCCAGC	2 cut(s)	3725, 4178
PspGI	CCWGG	6 cut(s)	615, 1009, 1623, 2434, 2760, 3972
PspLI	CGTACG	1 cut(s)	3917
PspPPI	RGGWCCY	1 cut(s)	1521
PspXI	VCTCGAGB	1 cut(s)	3893
PstI	CTGCAG	1 cut(s)	4531
PstNI	CAGNNNCTG	3 cut(s)	2125, 4205, 4511
PsuI	RGATCY	1 cut(s)	889
PsyI	GACNNNGTC	1 cut(s)	1084
PteI	GCGCGC	1 cut(s)	1650
RruI	TCGCGA	1 cut(s)	1051
RsaI	GTAC	7 cut(s)	363, 877, 944, 2500, 2963, 3358, 3919
RsaNI	GTAC	7 cut(s)	362, 876, 943, 2499, 2962, 3357, 3918
RseI	CAYNNNNRTG	4 cut(s)	845, 3684, 3738, 4565
SapI	GCTCTTC	2 cut(s)	372, 1593
SduI	GDGCHC	5 cut(s)	611, 1049, 1566, 3189, 3714
SfaNI	GCATC	7 cut(s)	2276, 3367, 3457, 3631, 3652, 4095, 4423
SfcI	CTRYAG	1 cut(s)	4527
SfoI	GGCGCC	4 cut(s)	1186, 1320, 1375, 2088
Sfr274I	CTCGAG	1 cut(s)	3893
SinI	GGWCC	8 cut(s)	1063, 1521, 2253, 2596, 3032, 3601, 3730, 3922
SlaI	CTCGAG	1 cut(s)	3893
SmiMI	CAYNNNNRTG	4 cut(s)	845, 3684, 3738, 4565
SmlI	CTYRAG	5 cut(s)	1425, 1988, 2205, 3893, 4244
SmoI	CTYRAG	5 cut(s)	1425, 1988, 2205, 3893, 4244
SseBI	AGGCCT	1 cut(s)	4369
SspDI	GGCGCC	4 cut(s)	1184, 1318, 1373, 2086
SspI	AATATT	1 cut(s)	2044
StuI	AGGCCT	1 cut(s)	4369
StyI	CCWWGG	8 cut(s)	1599, 1785, 2240, 2328, 3540, 3936, 4075, 4161
TaiI	ACGT	2 cut(s)	1856, 2927
TaqII	GACCGA	2 cut(s)	1029, 1514
TatI	WGTACW	3 cut(s)	361, 875, 2961
TfiI	GAWTC	9 cut(s)	837, 2200, 2236, 2407, 2510, 2987, 3743, 3757, 4489
TscAI	CASTG	8 cut(s)	459, 850, 1968, 2830, 4047, 4120, 4210, 4618
TseFI	GTSAC	9 cut(s)	172, 235, 1959, 2247, 2825, 2896, 3167, 3571, 4379
TseI	GCWGC	8 cut(s)	327, 813, 1096, 2303, 2654, 3380, 4175, 4459
Tsp45I	GTSAC	9 cut(s)	172, 235, 1959, 2247, 2825, 2896, 3167, 3571, 4379
TspGWI	ACGGA	5 cut(s)	385, 1361, 1467, 3942, 4627
TspRI	CASTG	8 cut(s)	459, 850, 1968, 2830, 4047, 4120, 4210, 4618
Tth111I	GACNNNGTC	1 cut(s)	1084
Van91I	CCANNNNNTGG	2 cut(s)	313, 4411
VpaK11BI	GGWCC	8 cut(s)	1063, 1521, 2253, 2596, 3032, 3601, 3730, 3922
XagI	CCTNNNNNAGG	1 cut(s)	293
XapI	RAATTY	7 cut(s)	199, 1895, 2337, 2685, 3059, 3127, 4329
XceI	RCATGY	2 cut(s)	666, 3344
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2392
XhoI	CTCGAG	1 cut(s)	3893
XmiI	GTMKAC	3 cut(s)	443, 624, 914
XmnI	GAANNNNTTC	6 cut(s)	23, 1151, 2430, 2514, 4023, 4053
Zsp2I	ATGCAT	1 cut(s)	341

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.